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https://www.readbyqxmd.com/read/27806708/ryanodine-induced-vasoconstriction-of-the-gerbil-spiral-modiolar-artery-depends-on-the-ca-2-sensitivity-but-not-on-ca-2-sparks-or-bk-channels
#1
Gayathri Krishnamoorthy, Katrin Reimann, Philine Wangemann
BACKGROUND: In many vascular smooth muscle cells (SMCs), ryanodine receptor-mediated Ca(2+) sparks activate large-conductance Ca(2+)-activated K(+) (BK) channels leading to lowered SMC [Ca(2+)]i and vasodilation. Here we investigated whether Ca(2+) sparks regulate SMC global [Ca(2+)]i and diameter in the spiral modiolar artery (SMA) by activating BK channels. METHODS: SMAs were isolated from adult female gerbils, loaded with the Ca(2+)-sensitive flourescent dye fluo-4 and pressurized using a concentric double-pipette system...
November 2, 2016: BMC Physiology
https://www.readbyqxmd.com/read/27513829/8-anilino-1-naphthalenesulfonate-layered-double-hydroxide-ultrathin-films-small-anion-assembly-and-its-potential-application-as-a-fluorescent-biosensor
#2
Ping Zhang, Ling Li, Yun Zhao, Zeyun Tian, Yumei Qin, Jun Lu
The fluorescent dye 8-anilino-1-naphthalenesulfonate (ANS) is a widely used fluorescent probe molecule for biochemistry analysis. This paper reported the fabrication of ANS/layered double hydroxide nanosheets (ANS/LDH)n ultrathin films (UTFs) via the layer-by-layer small anion assembly technique based on electrostatic interaction and two possible weak interactions: hydrogen-bond and induced electrostatic interactions between ANS and positive-charged LDH nanosheets. The obtained UTFs show a long-range-ordered periodic layered stacking structure and weak fluorescence in dry air or water, but it split into three narrow strong peaks in a weak polarity environment induced by the two-dimensional (2D) confinement effect of the LDH laminate; the fluorescence intensity increases with decreasing the solvent polarity, concomitant with the blue shift of the emission peaks, which show good sensoring reversibility...
September 6, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27314649/moringa-oleifera-s-nutritious-aqueous-leaf-extract-has-anticancerous-effects-by-compromising-mitochondrial-viability-in-an-ros-dependent-manner
#3
Niveen Madi, Mohammed Dany, Salah Abdoun, Julnar Usta
INTRODUCTION: Moringa oleifera (MO) is an important dietary component for many populations in West Africa and the Indian subcontinent. In addition to its highly nutritious value, almost all parts of this plant have been widely used in folk medicine in curing infectious, cardiovascular, gastrointestinal, hepatic, and other diseases. Evidence-based research supported its versatile medicinal properties; however, more rigorous research is required to establish it in cancer therapy. As such, in this study we aim to investigate the in vitro anticancerous effect of Moringa oleifera's aqueous leaf extract...
June 17, 2016: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/27221644/a-label-free-fluorescent-adenosine-triphosphate-biosensor-via-overhanging-aptamer-triggered-enzyme-protection-and-target-recycling-amplification
#4
Zhaoyin Wang, Jian Zhao, Zhihui Dai
Herein, a label-free fluorescent adenosine triphosphate (ATP) aptasensor is fabricated with a DNA hairpin and an overhanging aptamer. In the presence of ATP, the overhanging sequences of the aptamer may form preferred substrates of exo III, and thus trigger the enzyme-assisted amplification, which results in the release of G-rich sequences. Free G-rich sequences subsequently generate an enhanced flourescent signal by binding with thioflavin T. However, if ATP is absent, the overhanging sequence can induce steric hindrance and protect the DNA hairpin against the digestion of exo III, significantly reducing the noise of this biosensor...
June 20, 2016: Analyst
https://www.readbyqxmd.com/read/26891480/small-interfering-rna-delivery-through-positively-charged-polymer-nanoparticles
#5
Luca Dragoni, Raffaele Ferrari, Monica Lupi, Alberto Cesana, Francesca Falcetta, Paolo Ubezio, Maurizio D'Incalci, Massimo Morbidelli, Davide Moscatelli
Small interfering RNA (siRNA) is receiving increasing attention with regard to the treatment of many genetic diseases, both acquired and hereditary, such as cancer and diabetes. Being a high molecular weight (MW) polyanion, siRNA is not able to cross a cell membrane, and in addition it is unstable in physiological conditions. Accordingly, a biocompatible nanocarrier able to deliver siRNA into cells is needed. In this work, we synthesized biocompatible positively charged nanoparticles (NPs) following a two-step process that involves ring opening polymerization (ROP) and emulsion free radical polymerization (EFRP)...
March 29, 2016: Nanotechnology
https://www.readbyqxmd.com/read/26642654/methotrexate-and-myotrexate-induce-apoptosis-in-human-myoma-fibroblasts-t-hes-cell-line-via-mitochondrial-pathway
#6
Tatjana Kastratović, Slobodan Arsenijević, Zoran Matović, Marina Mitrović, Ivana Nikolić, Zoran Milosavljević, Zoran Protrka, Marija Šorak, Janko Đurić
Uterine leiomyomas (fibroids) are the most common benign tumors in women of reproductive age. Although the local application of low doses of methotrexate (MTX) is used as an effective treatment of the myomas, myotrexate could be a promising new drug. This study investigated the cytotoxic and apoptotic effects of both MTX and myotrexate in human fibroblasts derived from the uterine fibroids (T hES cell line). The myotrexate adduct is an aqueous solution of MTX and L-arginine. Cells were treated with a graded concentrations of both MTX and myothrexate (0...
May 2015: Acta Poloniae Pharmaceutica
https://www.readbyqxmd.com/read/26539832/molecular-subtype-specific-expression-of-microrna-29c-in-breast-cancer-is-associated-with-cpg-dinucleotide-methylation-of-the-promoter
#7
Elizabeth Poli, Jing Zhang, Chika Nwachukwu, Yonglan Zheng, Babatunde Adedokun, Olufunmilayo I Olopade, Yoo-Jeong Han
Basal-like breast cancer is a molecularly distinct subtype of breast cancer that is highly aggressive and has a poor prognosis. MicroRNA-29c (miR-29c) has been shown to be significantly down-regulated in basal-like breast tumors and to be involved in cell invasion and sensitivity to chemotherapy. However, little is known about the genetic and regulatory factors contributing to the altered expression of miR-29c in basal-like breast cancer. We here report that epigenetic modifications at the miR-29c promoter, rather than copy number variation of the gene, may drive the lower expression of miR-29c in basal-like breast cancer...
2015: PloS One
https://www.readbyqxmd.com/read/26353742/new-approach-way-using-substituent-group-at-core-chromophore-for-solution-process-blue-emitter
#8
Sunmi Lee, Seungho Kim, Jaehyun Lee, Beomjin Kim, Jongwook Park
Comparing the conventional vapor desposition process for OLEDs, the solution process using small molecules has merits of low production cost because of many reasons. For the solution process blue flourescent material, tertiary butyl (T) and anthracene (A) were first introduced as substituents to TAT core part, 2-tert-butyl-9,10-bis(3",5"-diphenylbiphenyl-4'-yl)anthracene (T-TAT) and 2-(9-anthracenyl)-9,10-bis(3",5"-diphenylbiphenyl-4'-yl)anthracene (A-TAT). All three materials indicated typical absorption band of anthracene in the range of 350 to 400 nm...
February 2015: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/26308792/qualitative-and-quantitative-evaluation-of-the-bcr-abl-fusion-gene-in-chronic-myelogenous-leukemia-by-flourescence-in-situ-hybridization-and-molecular-genetic-methods
#9
Sebnem Ozemri Sag, Tahsin Yakut, Ozlem Gorukmez, Orhan Gorukmez, Mehmet Ture, Mutlu Karkucak, Tuna Gulten, Ridvan Ali
AIMS: Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis. RESULTS: We analyzed 135 results for 78 CML patients tested by FISH and QRT-PCR for the Ph chromosomal translocation...
October 2015: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/26287997/infection-of-bacterial-endosymbionts-in-insects-a-comparative-study-of-two-techniques-viz-pcr-and-fish-for-detection-and-localization-of-symbionts-in-whitefly-bemisia-tabaci
#10
COMPARATIVE STUDY
Harpreet Singh Raina, Ambika Singh, Sonam Popli, Neeti Pandey, Raman Rajagopal
Bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. Different approaches have been used to understand such symbiont- host interactions. The whitefly, Bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. These bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. In this study, we have tried to compare two techniques, Polymerase chain reaction (PCR) and Flourescence in situ Hybridisation (FISH) commonly used for identification and localization of bacterial endosymbionts in B...
2015: PloS One
https://www.readbyqxmd.com/read/26111926/peptide-functionalized-zirconia-and-new-zirconia-titanium-biocermets-for-dental-applications
#11
Elisa Fernandez-Garcia, Xi Chen, Carlos F Gutierrez-Gonzalez, Adolfo Fernandez, Sonia Lopez-Esteban, Conrado Aparicio
OBJECTIVE: Titanium materials have been functionalized with biomolecules as a modern strategy to incorporate bioactive motifs that will expand and improve their biomedical applications. Here, we have biofunctionalized biomaterials based on zirconia of much interest for dentistry: the widely used bioceramic 3Y-TZP and a newly developed 3Y-TZP/Ti biocermet. METHODS: The biosurfaces were activated, silanized, and functionalized with coatings made of oligopeptides. Surface activation by plasma or alkaline-etching was optimized...
September 2015: Journal of Dentistry
https://www.readbyqxmd.com/read/26096031/fish-and-array-cgh-characterization-of-de-novo-derivative-y-chromosome-yq-duplication-and-partial-yp-deletion-in-an-azoospermic-male
#12
Ewa Wiland, Alexander N Yatsenko, Archana Kishore, Halina Stanczak, Agata Zdarta, Marcin Ligaj, Marta Olszewska, Jan Karol Wolski, Maciej Kurpisz
This study presents a 28-year-old infertile male who was referred to the cytogenetic laboratory for chromosomal analysis after 4 years of regular unprotected intercourse in whom non-obstructive azoospermia was revealed. Standard cytogenetic G-banding was performed on metaphase spreads and a de-novo karyotype 46,X,der(Y)(q11.22;p11.3) was identified. This analysis was followed by flourescence in-situ hybridization(FISH) and array comparative genomic hybridization (aCGH). Finally, the patient's karyotype was identified as 46,X,der(Y)(qter→q11...
August 2015: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/25965265/hepatitis-c-virus-ns3-mediated-microglial-inflammation-via-tlr2-tlr6-myd88-nf-%C3%AE%C2%BAb-pathway-and-toll-like-receptor-ligand-treatment-furnished-immune-tolerance
#13
Ayilam Ramachandran Rajalakshmy, Jambulingam Malathi, Hajib Naraharirao Madhavan
BACKGROUND: Recent evidence suggests the neurotrophic potential of hepatitis C virus (HCV). HCV NS3 protein is one of the potent antigens of this virus mediating inflammatory response in different cell types. Microglia being the immune surveillance cells in the central nervous system (CNS), the inflammatory potential of NS3 on microglia was studied. Role of toll like receptor (TLR) ligands Pam2CSK3 and Pam3CSK4 in controlling the NS3 mediated microglial inflammation was studied using microglial cell line CHME3...
2015: PloS One
https://www.readbyqxmd.com/read/25726586/-preparation-and-vitality-detection-of-protoplast-in-salvia-miltiorrhiza-bunge
#14
Nan Zhu, Jun Liu, Xinyu Zhang, Juan'e Dong
We prepared protoplasts from Salvia miltiorrhiza Bunge suspension culture cells. Then, the protoplasts' vitality and functions were tested by fluorescein diacetate staining method and Fluo-3/AM flourescent probe. The optimal condition of protoplast isolation was Cellulase R-10 1.5%, Pectinase Y-23 0.3%, Macerozyme R-10 0.5%, 40 r/min 12 h, 600 r/min 5 min, and the protoplasts yield was 1.1x10(6) cells/g FW, the vitality was more than 95% by using fluorescein diacetate staining method. It has been confirmed that calcium fluorescent probe Fluo-3/AM can be successfully loaded into protoplasts...
October 2014: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/25656128/-tracing-study-of-nanometer-microspheres-labeled-with-fluorescent-dye-on-gastric-cancer-cells
#15
Jie Ding, Min Feng, Wenxian Zhi, Wenxian Guan
OBJECTIVE: To explore the tracing role of nanometer microspheres tagged by fluorescent dye NIR-797 in gastric cancer cells in order to guide precise resection for gastric cancer. METHODS: Targeted polymer segment NH2-PEG-Pep-PCL and non-targeted polymer segment NH2-PEG- PCL(without Pep) were synthesized with NH2-PEG-NH2, gelatinase substrate peptides (Pep) and PCL-COOH by amidation method. Then nanometer microspheres were prepared by self-assemebly method. NIR-797 was linked into the end of PEG through chemical reaction...
February 2015: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/25638605/biophysical-interactions-of-novel-oleic-acid-conjugate-and-its-anticancer-potential-in-hela-cells
#16
Azmat Ali Khan, Amer M Alanazi, Mumtaz Jabeen, Mohammad Khalid Parvez, Khalid Pervez, Rizwan Wahab, Ali Saber Abdelhameed, Arun Chauhan
Novel conjugate 2,6-Diisopropylphenol-Oleic acid (2,6P-OLA) has shown potent anticancer activity on various cancer cell lines (Khan et al. Lipids 47:973-986, 2012). In the present study, the protein-or/ and DNA-binding property of 2,6P-OLA was evaluated that could predict its potential toxic effect, in vitro. Preferential structural stability and interaction mechanism of 2,6P-OLA to human serum albumin (HSA) and calf thymus DNA (CT-DNA) was used as model molecules, employing fluorescence spectroscopy (FS) and circular dichroism (CD) methods...
May 2015: Journal of Fluorescence
https://www.readbyqxmd.com/read/25518595/-morphological-characteristics-and-gene-expression-of-chrysomya-megacephala-eggs-in-different-developmental-stages
#17
Bing Xia, Yu-ming Liu, Qi-yan Wang, Hong-ling Zhang, Jie Wang, Jia-lin Dai, Jiang Huang
OBJECTIVE: To research the morphological characteristics and differential gene expression of Chrysomya megacephala eggs in different developmental stages. METHODS: After C. megacephala laid eggs (0 h), the eggs were collected every 2 h until eggs hatched into larvae. The morphological characteristics of C. megacephala eggs in different developmental stages were observed by stereo microscopy and scanning electron microscopy. The total RNA of the fly eggs was extracted...
August 2014: chinese Journal of Parasitology & Parasitic Diseases
https://www.readbyqxmd.com/read/25403583/concomitant-alk-translocation-and-egfr-mutation-in-lung-cancer-a-comparison-of-direct-sequencing-and-sensitive-assays-and-the-impact-on-responsiveness-to-tyrosine-kinase-inhibitor
#18
COMPARATIVE STUDY
J K Won, B Keam, J Koh, H J Cho, Y K Jeon, T M Kim, S H Lee, D S Lee, D W Kim, D H Chung
BACKGROUND: Epidermal growth factor receptor (EGFR) mutation and anaplastic lymphoma kinase (ALK) translocation are considered mutually exclusive in nonsmall-cell lung cancer (NSCLC). However, sporadic cases having concomitant EGFR and ALK alterations have been reported. The present study aimed to assess the prevalence of NSCLCs with concomitant EGFR and ALK alterations using mutation detection methods with different sensitivity and to propose an effective diagnostic and therapeutic strategy...
February 2015: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/25314615/picosecond-resolved-fluorescence-studies-of-substrate-and-cofactor-binding-domain-mutants-in-a-thermophilic-alcohol-dehydrogenase-uncover-an-extended-network-of-communication
#19
Corey W Meadows, Jonathan E Tsang, Judith P Klinman
Time-resolved fluorescence dynamics are investigated in two mutants of a thermophilic alcohol dehydrogenase (ht-ADH): Y25A (at the dimer interface) and V260A (at the cofactor-binding domain). These residues, ca. 32 Å apart, are shown to exhibit opposing low-temperature effects on the hydride tunneling step. Using single-tryptophan constructs at the active site (Trp87) and a remote, surface-exposed site (Trp167), time-dependent Stokes shifts and collisional quenching data allow an analysis of intra-protein dynamical communication...
October 22, 2014: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/25280052/effect-of-cochlear-nerve-electrocautery-on-the-adult-cochlear-nucleus
#20
Claire E Iseli, William H Merwin, Cristine Klatt-Cromwell, Kendall A Hutson, Matthew G Ewend, Oliver F Adunka, Douglas C Fitzpatrick, Craig A Buchman
HYPOTHESIS: Electrocauterization and subsequent transection of the cochlear nerve induce greater injury to the cochlear nucleus than sharp transection alone. BACKGROUND: Some studies show that neurofibromatosis Type 2 (NF2) patients fit with auditory brainstem implants (ABIs) fail to achieve speech perception abilities similar to ABI recipients without NF2. Reasons for these differences remain speculative. One hypothesis posits poorer performance to surgically induced trauma to the cochlear nucleus from electrocautery...
April 2015: Otology & Neurotology
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