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https://www.readbyqxmd.com/read/28525954/construction-of-various-%C3%AE-34-5-deleted-fluorescent-expressing-oncolytic-herpes-simplex-type-1-ohsv-for-generation-and-isolation-of-hsv-based-vectors
#1
Shahriyar Abdoli, Farzin Roohvand, Ladan Teimoori-Toolabi, Mohammad Ali Shokrgozar, Mina Bahrololoumi, Kayhan Azadmanesh
Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the construction of three single/dually-flourescence labeled and γ34.5-deleted, recombinant HSV-1 vectors for rapid generation and easy selection/isolation of different HSV-Based vectors. Methods: Generation of recombinant viruses was performed with conventional homologous recombination methods using green fluorescent protein (GFP) and BleCherry harboring shuttle vectors...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28443520/recent-advances-on-3-hydroxyflavone-derivatives-structures-and-properties
#2
Burcu Butun, Gulacti Topcu, Turan Ozturk
Flavonoids are naturally occurring phenolic compounds named as 2-phenyl-1-benzopyran-4-one and classified into several subclasses according to their basic chemical structures such as flavones, flavonols, flavanones, flavanonols, and flavanols. Among them, flavonols (3-hydroxyflavone), containing a 3-hydroxyl group in association with its 4-keto group and double bond between 2- and 3- carbon atoms, have the best electron conjugated skeleton in flavonoid family, providing several resonance structures and chelating ability, through which various pharmacological activities such as antiviral, antitumor, anti-inflammatory, anticholinesterase, cytotoxicity, and particularly high antioxidant activity are demonstrated...
April 24, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28372570/malaria-over-diagnosis-in-cameroon-diagnostic-accuracy-of-fluorescence-and-staining-technologies-fast-malaria-stain-and-led-microscopy-versus-giemsa-and-bright-field-microscopy-validated-by-polymerase-chain-reaction
#3
Sean M Parsel, Steven A Gustafson, Edward Friedlander, Alexander A Shnyra, Aderosoye J Adegbulu, Ying Liu, Nicole M Parrish, Syed A Jamal, Eve Lofthus, Leo Ayuk, Charles Awasom, Carolyn J Henry, Carole P McArthur
BACKGROUND: Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where Plasmodium species are indigenous, there are multiple etiologies of fever leading to misdiagnoses, especially in populations where HIV is prevalent and children. To determine the frequency of malaria infection in febrile patients over an 8-month period at the Regional Hospital in Bamenda, Cameroon, we evaluated the clinical efficacy of the Flourescence and Staining Technology (FAST) Malaria stain and ParaLens Advance(TM) microscopy system (FM) and compared it with conventional bright field microscopy and Giemsa stain (GS)...
April 4, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28272880/nitration-of-tyrosine-residue-y10-of-a%C3%AE-1-42-significantly-inhibits-its-aggregation-and-cytotoxicity
#4
Jie Zhao, Jinming Wu, Zhen Yang, Hailing Li, Zhonghong Gao
Amyloid-β plaques and oxidative stress are the major hallmarks of Alzheimer's disease. Our previous study found that the heme-Aβ complex enhanced the catalytic effect of free heme on protein tyrosine nitration in the presence of hydrogen peroxide (H2O2) and nitrite (NO2(-)). Y10 in Aβ could be the first target to be nitrated. We also found that nitration of Aβ1-40 significantly decreased its aggregation. However, a contrary report showed that nitration of Aβ1-42 by peroxynitrite enhanced its aggregation...
April 17, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28147239/a-sensitive-alk-immunohistochemistry-companion-diagnostic-test-identifies-patients-eligible-for-treatment-with-crizotinib
#5
Trish Thorne-Nuzzo, Crystal Williams, Alice Catallini, June Clements, Shalini Singh, James Amberson, Kim Dickinson, Zoran Gatalica, Steffan N Ho, Isabell Loftin, Abigail McElhinny, Penny Towne
INTRODUCTION: The availability of high-quality, rigorously validated diagnostic tests that can be broadly implemented is necessary to efficiently identify patients with anaplastic lymphoma kinase (ALK)-positive NSCLC who can potentially benefit from treatment with crizotinib. Here we present data on the recently approved Ventana ALK (D5F3) CDx Assay (Ventana Medical Systems, Tucson, AZ), the only immunohistochemistry (IHC)-based assay linked to treatment outcome. METHODS: NSCLC specimens prospectively tested for anaplastic lymphoma receptor tyrosine kinase gene (ALK) status by flourescent in situ hybridization (FISH) in the PROFILE 1014 clinical trial of crizotinib versus chemotherapy (N = 1018, including 179 ALK-positive and 754 ALK-negative specimens) were evaluated using the ALK (D5F3) CDx assay...
May 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28124963/-evaluation-of-pna-fish-method-for-direct-identification-of-candida-species-in-blood-culture-samples-and-its-potential-impact-on-guidance-of-antifungal-therapy
#6
Özlem Doğan, Ahmet Çağkan İnkaya, Dolunay Gülmez, Ömrüm Uzun, Murat Akova, Sevtap Arıkan Akdağlı
Early antifungal therapy has a major influence on survival in candidemia. Rapid identification of the species has importance for the treatment, prediction of the species-specific primary resistance and variable antifungal susceptibility. Recently, molecular-based methods attempt to reduce the time between the positive signal of a blood culture and identification of the fungus. PNA-FISH (Peptide nucleic acid fluorescence in situ hybridization) assay distinguishes a number of frequently isolated Candida species in groups following the growth in blood culture...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28113123/disruption-of-gel-phase-lipid-packing-efficiency-by-sucralose-studied-with-merocyanine-540
#7
Morgan Barker, Anthony Kennedy
Sucralose, an artificial sweetener, displays very different behavior towards membranes than its synthetic precursor sucrose. The impact of both sugars on model dipalmitoylphosphatidylcholine model membranes was investigated using absorbance and flourescence spectroscopy and the membrane probe merocyanine 540. This probe molecule is highly sensitive to changes in membrane packing, microviscosity and polarity. This work focuses on the impact of sugars on the outer leaflet of unilamellar dipalmitoyl phosphatidylcholine model membranes...
April 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27806708/ryanodine-induced-vasoconstriction-of-the-gerbil-spiral-modiolar-artery-depends-on-the-ca-2-sensitivity-but-not-on-ca-2-sparks-or-bk-channels
#8
Gayathri Krishnamoorthy, Katrin Reimann, Philine Wangemann
BACKGROUND: In many vascular smooth muscle cells (SMCs), ryanodine receptor-mediated Ca(2+) sparks activate large-conductance Ca(2+)-activated K(+) (BK) channels leading to lowered SMC [Ca(2+)]i and vasodilation. Here we investigated whether Ca(2+) sparks regulate SMC global [Ca(2+)]i and diameter in the spiral modiolar artery (SMA) by activating BK channels. METHODS: SMAs were isolated from adult female gerbils, loaded with the Ca(2+)-sensitive flourescent dye fluo-4 and pressurized using a concentric double-pipette system...
November 2, 2016: BMC Physiology
https://www.readbyqxmd.com/read/27513829/8-anilino-1-naphthalenesulfonate-layered-double-hydroxide-ultrathin-films-small-anion-assembly-and-its-potential-application-as-a-fluorescent-biosensor
#9
Ping Zhang, Ling Li, Yun Zhao, Zeyun Tian, Yumei Qin, Jun Lu
The fluorescent dye 8-anilino-1-naphthalenesulfonate (ANS) is a widely used fluorescent probe molecule for biochemistry analysis. This paper reported the fabrication of ANS/layered double hydroxide nanosheets (ANS/LDH)n ultrathin films (UTFs) via the layer-by-layer small anion assembly technique based on electrostatic interaction and two possible weak interactions: hydrogen-bond and induced electrostatic interactions between ANS and positive-charged LDH nanosheets. The obtained UTFs show a long-range-ordered periodic layered stacking structure and weak fluorescence in dry air or water, but it split into three narrow strong peaks in a weak polarity environment induced by the two-dimensional (2D) confinement effect of the LDH laminate; the fluorescence intensity increases with decreasing the solvent polarity, concomitant with the blue shift of the emission peaks, which show good sensoring reversibility...
September 6, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27314649/moringa-oleifera-s-nutritious-aqueous-leaf-extract-has-anticancerous-effects-by-compromising-mitochondrial-viability-in-an-ros-dependent-manner
#10
Niveen Madi, Mohammed Dany, Salah Abdoun, Julnar Usta
INTRODUCTION: Moringa oleifera (MO) is an important dietary component for many populations in West Africa and the Indian subcontinent. In addition to its highly nutritious value, almost all parts of this plant have been widely used in folk medicine in curing infectious, cardiovascular, gastrointestinal, hepatic, and other diseases. Evidence-based research supported its versatile medicinal properties; however, more rigorous research is required to establish it in cancer therapy. As such, in this study we aim to investigate the in vitro anticancerous effect of Moringa oleifera's aqueous leaf extract...
September 2016: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/27221644/a-label-free-fluorescent-adenosine-triphosphate-biosensor-via-overhanging-aptamer-triggered-enzyme-protection-and-target-recycling-amplification
#11
Zhaoyin Wang, Jian Zhao, Zhihui Dai
Herein, a label-free fluorescent adenosine triphosphate (ATP) aptasensor is fabricated with a DNA hairpin and an overhanging aptamer. In the presence of ATP, the overhanging sequences of the aptamer may form preferred substrates of exo III, and thus trigger the enzyme-assisted amplification, which results in the release of G-rich sequences. Free G-rich sequences subsequently generate an enhanced flourescent signal by binding with thioflavin T. However, if ATP is absent, the overhanging sequence can induce steric hindrance and protect the DNA hairpin against the digestion of exo III, significantly reducing the noise of this biosensor...
June 20, 2016: Analyst
https://www.readbyqxmd.com/read/26891480/small-interfering-rna-delivery-through-positively-charged-polymer-nanoparticles
#12
Luca Dragoni, Raffaele Ferrari, Monica Lupi, Alberto Cesana, Francesca Falcetta, Paolo Ubezio, Maurizio D'Incalci, Massimo Morbidelli, Davide Moscatelli
Small interfering RNA (siRNA) is receiving increasing attention with regard to the treatment of many genetic diseases, both acquired and hereditary, such as cancer and diabetes. Being a high molecular weight (MW) polyanion, siRNA is not able to cross a cell membrane, and in addition it is unstable in physiological conditions. Accordingly, a biocompatible nanocarrier able to deliver siRNA into cells is needed. In this work, we synthesized biocompatible positively charged nanoparticles (NPs) following a two-step process that involves ring opening polymerization (ROP) and emulsion free radical polymerization (EFRP)...
March 29, 2016: Nanotechnology
https://www.readbyqxmd.com/read/26642654/methotrexate-and-myotrexate-induce-apoptosis-in-human-myoma-fibroblasts-t-hes-cell-line-via-mitochondrial-pathway
#13
Tatjana Kastratović, Slobodan Arsenijević, Zoran Matović, Marina Mitrović, Ivana Nikolić, Zoran Milosavljević, Zoran Protrka, Marija Šorak, Janko Đurić
Uterine leiomyomas (fibroids) are the most common benign tumors in women of reproductive age. Although the local application of low doses of methotrexate (MTX) is used as an effective treatment of the myomas, myotrexate could be a promising new drug. This study investigated the cytotoxic and apoptotic effects of both MTX and myotrexate in human fibroblasts derived from the uterine fibroids (T hES cell line). The myotrexate adduct is an aqueous solution of MTX and L-arginine. Cells were treated with a graded concentrations of both MTX and myothrexate (0...
May 2015: Acta Poloniae Pharmaceutica
https://www.readbyqxmd.com/read/26539832/molecular-subtype-specific-expression-of-microrna-29c-in-breast-cancer-is-associated-with-cpg-dinucleotide-methylation-of-the-promoter
#14
Elizabeth Poli, Jing Zhang, Chika Nwachukwu, Yonglan Zheng, Babatunde Adedokun, Olufunmilayo I Olopade, Yoo-Jeong Han
Basal-like breast cancer is a molecularly distinct subtype of breast cancer that is highly aggressive and has a poor prognosis. MicroRNA-29c (miR-29c) has been shown to be significantly down-regulated in basal-like breast tumors and to be involved in cell invasion and sensitivity to chemotherapy. However, little is known about the genetic and regulatory factors contributing to the altered expression of miR-29c in basal-like breast cancer. We here report that epigenetic modifications at the miR-29c promoter, rather than copy number variation of the gene, may drive the lower expression of miR-29c in basal-like breast cancer...
2015: PloS One
https://www.readbyqxmd.com/read/26353742/new-approach-way-using-substituent-group-at-core-chromophore-for-solution-process-blue-emitter
#15
Sunmi Lee, Seungho Kim, Jaehyun Lee, Beomjin Kim, Jongwook Park
Comparing the conventional vapor desposition process for OLEDs, the solution process using small molecules has merits of low production cost because of many reasons. For the solution process blue flourescent material, tertiary butyl (T) and anthracene (A) were first introduced as substituents to TAT core part, 2-tert-butyl-9,10-bis(3",5"-diphenylbiphenyl-4'-yl)anthracene (T-TAT) and 2-(9-anthracenyl)-9,10-bis(3",5"-diphenylbiphenyl-4'-yl)anthracene (A-TAT). All three materials indicated typical absorption band of anthracene in the range of 350 to 400 nm...
February 2015: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/26308792/qualitative-and-quantitative-evaluation-of-the-bcr-abl-fusion-gene-in-chronic-myelogenous-leukemia-by-flourescence-in-situ-hybridization-and-molecular-genetic-methods
#16
Sebnem Ozemri Sag, Tahsin Yakut, Ozlem Gorukmez, Orhan Gorukmez, Mehmet Ture, Mutlu Karkucak, Tuna Gulten, Ridvan Ali
AIMS: Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We compared the diagnostic utility of FISH and QRT-PCR at various time points in the course of chronic myelogenous leukemia (CML) and to determine the mean initial values for patients whose QRT-PCR results were not known at the time of diagnosis. RESULTS: We analyzed 135 results for 78 CML patients tested by FISH and QRT-PCR for the Ph chromosomal translocation...
October 2015: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/26287997/infection-of-bacterial-endosymbionts-in-insects-a-comparative-study-of-two-techniques-viz-pcr-and-fish-for-detection-and-localization-of-symbionts-in-whitefly-bemisia-tabaci
#17
COMPARATIVE STUDY
Harpreet Singh Raina, Ambika Singh, Sonam Popli, Neeti Pandey, Raman Rajagopal
Bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. Different approaches have been used to understand such symbiont- host interactions. The whitefly, Bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. These bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. In this study, we have tried to compare two techniques, Polymerase chain reaction (PCR) and Flourescence in situ Hybridisation (FISH) commonly used for identification and localization of bacterial endosymbionts in B...
2015: PloS One
https://www.readbyqxmd.com/read/26111926/peptide-functionalized-zirconia-and-new-zirconia-titanium-biocermets-for-dental-applications
#18
Elisa Fernandez-Garcia, Xi Chen, Carlos F Gutierrez-Gonzalez, Adolfo Fernandez, Sonia Lopez-Esteban, Conrado Aparicio
OBJECTIVE: Titanium materials have been functionalized with biomolecules as a modern strategy to incorporate bioactive motifs that will expand and improve their biomedical applications. Here, we have biofunctionalized biomaterials based on zirconia of much interest for dentistry: the widely used bioceramic 3Y-TZP and a newly developed 3Y-TZP/Ti biocermet. METHODS: The biosurfaces were activated, silanized, and functionalized with coatings made of oligopeptides. Surface activation by plasma or alkaline-etching was optimized...
September 2015: Journal of Dentistry
https://www.readbyqxmd.com/read/26096031/fish-and-array-cgh-characterization-of-de-novo-derivative-y-chromosome-yq-duplication-and-partial-yp-deletion-in-an-azoospermic-male
#19
Ewa Wiland, Alexander N Yatsenko, Archana Kishore, Halina Stanczak, Agata Zdarta, Marcin Ligaj, Marta Olszewska, Jan Karol Wolski, Maciej Kurpisz
This study presents a 28-year-old infertile male who was referred to the cytogenetic laboratory for chromosomal analysis after 4 years of regular unprotected intercourse in whom non-obstructive azoospermia was revealed. Standard cytogenetic G-banding was performed on metaphase spreads and a de-novo karyotype 46,X,der(Y)(q11.22;p11.3) was identified. This analysis was followed by flourescence in-situ hybridization(FISH) and array comparative genomic hybridization (aCGH). Finally, the patient's karyotype was identified as 46,X,der(Y)(qter→q11...
August 2015: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/25965265/hepatitis-c-virus-ns3-mediated-microglial-inflammation-via-tlr2-tlr6-myd88-nf-%C3%AE%C2%BAb-pathway-and-toll-like-receptor-ligand-treatment-furnished-immune-tolerance
#20
Ayilam Ramachandran Rajalakshmy, Jambulingam Malathi, Hajib Naraharirao Madhavan
BACKGROUND: Recent evidence suggests the neurotrophic potential of hepatitis C virus (HCV). HCV NS3 protein is one of the potent antigens of this virus mediating inflammatory response in different cell types. Microglia being the immune surveillance cells in the central nervous system (CNS), the inflammatory potential of NS3 on microglia was studied. Role of toll like receptor (TLR) ligands Pam2CSK3 and Pam3CSK4 in controlling the NS3 mediated microglial inflammation was studied using microglial cell line CHME3...
2015: PloS One
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