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https://www.readbyqxmd.com/read/29451996/foveal-avascular-zone-area-changes-analyzed-using-oct-angiography-after-successful-rhegmatogenous-retinal-detachment-repair
#1
Je Moon Woo, Yo Sep Yoon, Jong Eun Woo, Jung Kee Min
PURPOSE: To investigate foveal avascular zone (FAZ) area after surgery in patients with rhegmatogenous retinal detachment (RRD) that involved or uninvolved the macula, and to evaluate the correlation between FAZ area and visual outcomes using swept-source optical coherence tomography angiography (OCTA). MATERIALS AND METHODS: This retrospective case-control study included 34 eyes (34 patients) with recent onset RRD that were successfully repaired with a single, uncomplicated surgical procedure (pars plana vitrectomy with gas tamponade)...
February 16, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29448030/a-sep-tag-enhances-the-expression-solubility-and-yield-of-recombinant-tev-protease-without-altering-its-activity
#2
Kalpana Nautiyal, Yutaka Kuroda
Tobacco Etch Virus (TEV) protease is used in the purification of recombinant proteins, but its use is often hampered by solubility issues. Here, we report a short, 12-residue solubility enhancing peptide (SEP) tag attached at the C-terminus of TEV (TEV-C9R). We assessed the effects of the C9R tag on the biophysical and biochemical characteristics of TEV. The yield of HPLC purified TEV-C9R expressed in E. coli grown in 200 mL LB or TB media was between 10 and 13 mg, which was up to 6.5 times higher than the yield of the untagged TEV...
February 12, 2018: New Biotechnology
https://www.readbyqxmd.com/read/29445637/virulence-determinants-and-antimicrobial-resistance-patterns-of-vancomycin-resistant-enterococcus-faecium-isolated-from-different-sources-in-southwest-iran
#3
Maniya Arshadi, Mahmood Mahmoudi, Moloud Sadat Motahar, Saber Soltani, Mohammad Reza Pourmand
Background: This study aimed to investigate the incidence of antibiotic-resistance and virulence genes in vancomycin-resistant Enterococcus faecium isolated from different sources in southwest Iran from Mar to Sep 2015. Methods: Overall, 120 E. faecium isolates (80 VRE and 40 vancomycin-susceptible enterococci [VSE] isolates) were obtained from four hospitals. The resistance of the VRE isolates was determined by disk diffusion method. Multiplex PCR was performed to detect the virulence genes carried by the E...
February 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29442409/socioeconomic-status-and-multimorbidity-a-systematic-review-and-meta-analysis
#4
Thanya I Pathirana, Caroline A Jackson
OBJECTIVES: We performed a systematic review to identify, critically appraise and synthesise the existing literature on the association between SEP and multimorbidity occurrence. METHODS: We searched Medline and Embase from inception to December 2014. Where possible we performed meta-analysis to obtain summary odds ratios (ORs), exploring heterogeneity between studies through sub-group analysis. RESULTS: We identified 24 cross-sectional studies that largely reported on education, deprivation or income in relation to multimorbidity occurrence...
February 14, 2018: Australian and New Zealand Journal of Public Health
https://www.readbyqxmd.com/read/29438434/seasonal-dynamics-of-the-juvenile-fish-community-structure-in-the-maowei-sea-mangroves
#5
Zhi-Qiang Wu, Qi Zou, Tao Chang, Dong Zhang, Liang-Liang Huang
More than 50% of Chinese mangroves were lost between 1950 and 2000 to habitat destruction, prompting an urge for conservation. To assess the importance of the protected Maowei Gulf mangrove estuary for fish population assemblage in the Beibu Gulf (China), we studied species composition and abundance of juvenile fish (including larvae) from July 2012 to June 2013. A total of 11 691 specimens were collected, which belonged to 24 species and 15 families. Six perciform species constituted 93% of the total sample...
2018: PloS One
https://www.readbyqxmd.com/read/29433023/characterization-of-trace-metal-removal-products-in-vertical-flow-bioreactor-substrates-at-the-mayer-ranch-passive-treatment-system-in-the-tar-creek-superfund-site
#6
Julie A LaBar, Robert W Nairn
A passive treatment system (PTS), including two parallel vertical flow bioreactors (VFBR), was constructed in 2008 for the treatment of unabated net-alkaline ferruginous mine drainage in the Tar Creek Superfund Site in northeastern Oklahoma. Water quality data collected since the PTS began operation indicate significant removal of trace metals in the VFBR. Results of a sequential extraction procedure (SEP) performed on substrate samples showed that the majority of Cd, Co, Fe, Ni, Pb, and Zn were retained in the refractory organic/sulfide fraction...
February 3, 2018: Chemosphere
https://www.readbyqxmd.com/read/29429253/-diagnostic-significance-of-serum-free-dna-human-telomerase-reverse-transcriptase-quantitative-determination-on-spinal-cord-injury
#7
M K Yang, J Tang, Z Xiang, X Zhang, J Wang, Z Li, Y Li, W B Sheng
Objective: To investigate the relationship between the content of human telomerase reverse transcriptase (hTERT) and its clinical features in serum free DNA in patients with different degree of spinal cord injury. Methods: From December 2013 to December 2016, inpatients of the Central Hospital of Bazhong, Sichuan Province were enrolledand divided into the experimental group, the disease control group and the negative control group. For the experimental group: 46 patients with spinal cord injury were graded according to the criteria of the American Association of Spinal Cord Injury (ASIA), including 12 cases of grade A, 10 cases of grade B, 10 cases of grade C, 7 cases of grade D and 7 cases of grade E; for the disease control group: 15 patients with spinal fractures (without spinal cord injury) at the same period were included; and for the negative control group: 20 healthy adult volunteers aged 18-50 years were selected...
February 6, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29428265/first-line-management-of-chronic-subdural-hematoma-with-the-subdural-evacuating-port-system-institutional-experience-and-predictors-of-outcomes
#8
Haydn Hoffman, Robert Ziechmann, Timothy Beutler, Brendon Verhave, Lawrence S Chin
Chronic subdural hematoma (cSDH) is a common condition that disproportionately affects older patients. Given the greater risks of general anesthesia in this population, interest has turned towards less invasive surgical approaches such as the subdural evacuating port system (SEPS; Medtronic, Inc., Minneapolis, MN). There is a relative dearth of information about the outcomes following this procedure. Here, we present our institution's experience with SEPS and analyze factors associated with the outcomes. Using a prospectively maintained institutional database, we retrospectively identified all patients who presented with cSDH and received first line therapy with SEPS...
February 7, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29428172/social-influences-on-health-related-behaviour-clustering-during-adulthood-in-two-british-birth-cohort-studies
#9
Claire Mawditt, Amanda Sacker, Annie Britton, Yvonne Kelly, Noriko Cable
Building upon evidence linking socio-economic position (SEP) in childhood and adulthood with health-related behaviours (HRB) in adulthood, we examined how pre-adolescent SEP predicted membership of three HRB clusters: "Risky", "Moderate Smokers" and "Mainstream" (the latter pattern consisting of more beneficial HRBs), that were detected in our previous work. Data were taken from two British cohorts (born in 1958 and 1970) in pre-adolescence (age 11 and 10, respectively) and adulthood (age 33 and 34). SEP constructs in pre-adolescence and adulthood were derived through Confirmatory Factor Analysis...
February 8, 2018: Preventive Medicine
https://www.readbyqxmd.com/read/29427450/reconstruction-of-the-plantar-toe-with-a-distal-reverse-instep-sensory-island-flap
#10
Mohammad Ali Hosseinian, Ali Gharibi Loron, Behzad Nemati Honar
BACKGROUND: Plantar toe ulcers are a challenging surgical problem. There are several methods for reconstruction, but no attention has been given to the preservation of sensation. This report proposes a method to provide protective sensation for the reconstructed area. PATIENTS AND METHODS: The ulcers of seven patients ranged from 2 × 3 to 7 × 3 cm with defects of the plantar first toe and distal metatarsus, including four burns, a trauma, a diabetic ulcer, and a neuropathy injury reconstructed with a distal reverse instep sensory island (DRISI) flap...
February 10, 2018: Microsurgery
https://www.readbyqxmd.com/read/29424423/near-infrared-spectroscopic-models-for-analysis-of-winter-pea-pisum-sativum-l-quality-constituents
#11
Uttam Saha, Rachel A Vann, S Chris Reberg-Horton, Miguel S Castillo, Steven B Mirsky, Rebecca J McGee, Leticia Sonon
BACKGROUND: Winter pea (Pisum sativum L.) grows well in a wide geographic region both as a forage and cover crop. Understanding the quality constituents of this crop is important for both end uses; however, analysis of quality constituents by conventional wet chemistry methods is laborious, slow, and costly. Near Infrared Reflectance Spectroscopy (NIRS) is a precise, accurate, rapid, and cheap alternative to using wet chemistry to estimate quality constituents. We developed and validated NIRS calibration models for constituent analysis of this crop...
February 9, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29414299/predicting-falls-in-multiple-sclerosis-do-electrophysiological-measures-have-a-better-predictive-accuracy-compared-to-clinical-measures
#12
Somasundaram Aadhimoolam Chinnadurai, Divya Gandhirajan, Avathvadi Venkatesan Srinivasan, Bhanu Kesavamurthy, Lakshmi Narasimhan Ranganathan, Vijaya Pamidimukkala
BACKGROUND: The risk of falls in people with Multiple Sclerosis (MS) is much greater than that of the general population due to impaired coordination, gait, sensation, muscle tone, strength, and cognition. These MS related falls hamper the day to day living of these individuals and are one of the prime factors aggravating the disease related morbidity. The fear of falling itself may make these individuals more dependent and hinder their professional and leisurely activities. Hence, the significance of identifying individuals who are at risk of falling and instituting preventive counter-measures cannot be overemphasized...
February 2, 2018: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29413578/determination-of-pyrethrin-and-pyrethroid-residues-in-animal-fat-using-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#13
M Moloney, S Tuck, A Ramkumar, A Furey, M Danaher
A method was developed for the confirmatory and quantitative analysis of one pyrethrin and 18 pyrethroid residues in animal fat. Fat was extracted was collected from adipose tissue melted in an oven at 65 °C for 2 h. Fat samples (1 g) were dispersed with deactivated Florisil® sorbent and extracted with MeCN. Sample extracts were purified by cold temperature precipitation at -30 °C for 4 h and further purified using dispersive solid-phase extraction (d-SPE) clean-up in tubes containing 500 mg of Z-SEP+ and 125 mg of PSA bonded silica...
December 13, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29411873/modeling-transfer-of-vibrio-parahaemolyticus-during-peeling-of-raw-shrimp
#14
Xingning Xiao, Haiying Pang, Wen Wang, Weihuan Fang, Yingchun Fu, Yanbin Li
This study aimed to qualify the transfer of Vibrio parahaemolyticus during the shrimp peeling process via gloves under 3 different scenarios. The 1st 2 scenarios provided quantitative information for the probability distribution of bacterial transfer rates from (i) contaminated shrimp (6 log CFU/g) to non-contaminated gloves (Scenario 1) and (ii) contaminated gloves (6 log CFU/per pair) to non-contaminated shrimp (Scenario 2). In Scenario 3, bacterial transfer from contaminated shrimp to non-contaminated shrimp in the shrimp peeling process via gloves was investigated to develop a predictive model for describing the successive bacterial transfer...
February 7, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29406324/socioeconomic-position-and-age-related-disparities-in-regional-cerebral-blood-flow-within-the-prefrontal-cortex
#15
Daniel A Hackman, Dora C-H Kuan, Stephen B Manuck, Peter J Gianaros
OBJECTIVE: Socioeconomic position (SEP) is associated with cerebrovascular health and brain function, particularly in prefrontal cortex (PFC) and medial temporal lobe regions that exhibit plasticity across the lifecourse. However, it is unknown if SEP associates with resting cerebral blood flow (CBF), an indicator of baseline brain function, in these regions in midlife, and if the association is (1) Period-specific, with independent associations for childhood and adulthood SEP, or driven by lifecourse SEP; and (2) Explained by a persistent disparity, widening disparity, or the leveling of disparities with age...
February 6, 2018: Psychosomatic Medicine
https://www.readbyqxmd.com/read/29401013/new-risk-adjustment-policies-reduce-but-do-not-eliminate-special-enrollment-period-underpayment
#16
Stan Dorn, Bowen Garrett, Marni Epstein
Millions of uninsured Americans do not sign up for available coverage despite job loss or other factors that would make them eligible for special enrollment periods (SEPs). Such periods let people enroll in nongroup insurance outside the usual open enrollment period for Marketplace coverage. Concerned that risk adjustment results in underpayment for the health risks associated with SEP enrollees, carriers rarely market their products to consumers eligible for SEPs, and many do not pay agents and brokers to enroll such consumers...
February 2018: Health Affairs
https://www.readbyqxmd.com/read/29396604/socioeconomic-position-and-occupational-social-class-and-their-association-with-risky-alcohol-consumption-among-adolescents
#17
Núria Obradors-Rial, Carles Ariza, Luis Rajmil, Carles Muntaner
OBJECTIVES: To compare different measures of socioeconomic position (SEP) and occupational social class (OSC) and to evaluate their association with risky alcohol consumption among adolescents attending the last mandatory secondary school (ages 15-17 years). METHODS: This was a cross-sectional study. 1268 adolescents in Catalonia (Spain) participated in the study. Family affluence scale (FAS), parents' OSC, parents' level of education and monthly familiar income were used to compare socioeconomic indicators...
February 2, 2018: International Journal of Public Health
https://www.readbyqxmd.com/read/29393901/effects-of-heterologous-trna-modifications-on-the-production-of-proteins-containing-noncanonical-amino-acids
#18
Ana Crnković, Oscar Vargas-Rodriguez, Anna Merkuryev, Dieter Söll
Synthesis of proteins with noncanonical amino acids (ncAAs) enables the creation of protein-based biomaterials with diverse new chemical properties that may be attractive for material science. Current methods for large-scale production of ncAA-containing proteins, frequently carried out in Escherichia coli, involve the use of orthogonal aminoacyl-tRNA synthetases (o-aaRSs) and tRNAs (o-tRNAs). Although o-tRNAs are designed to be orthogonal to endogenous aaRSs, their orthogonality to the components of the E...
February 2, 2018: Bioengineering
https://www.readbyqxmd.com/read/29390510/differentiation-of-direct-and-indirect-socioeconomic-effects-on-suicide-attempts-in-south-korea
#19
Myung Ki, Eui Seong Sohn, Byungduck An, Jiseun Lim
Despite the wide recognition of the inverse association between socioeconomic position (SEP) and suicidal behaviors, its underlying process and potential mediators are little known. This study investigated the pathway from SEP to suicide attempts with attention to potential mediators.From the Korean Health and Nutrition Examination Survey 2007-2013, which is a nationwide cross-sectional survey of the health and nutritional status, a total of 34,565 participants (≥30 years) were included in the analysis. To unfold the pathways linking SEP to suicide attempts, the direct and indirect effects of 3 SEP measures (educational attainment, household income, and occupational group) and 3 mediators (physical illness, mental health problems, and problematic drinking) were differentiated using structured equation model (SEM)...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29388802/graphic-and-arousing-emotional-and-cognitive-reactions-to-tobacco-graphic-health-warnings-and-associated-quit-related-outcomes-among-low-sep-population-groups
#20
Mesfin Awoke Bekalu, Shoba Ramanadhan, Cabral A Bigman, Rebekah H Nagler, Kasisomayajula Viswanath
Research on graphic health warnings (GHWs) indicates that beyond changing cognitions about the health effects of smoking, GHWs evoke emotional reactions that can influence quit-related outcomes. Emotions can be classified based on valence (positive or negative) and arousal (calm or excited). However, although considerable research has examined the differential effectiveness of positive versus negative GHW-evoked emotions, research investigating the role of arousal activation in quit-related behaviors is scarce...
February 1, 2018: Health Communication
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