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https://www.readbyqxmd.com/read/29160218/antimicrobial-resistance-heavy-metal-resistance-and-integron-content-in-bacteria-isolated-from-a-south-african-tilapia-aquaculture-system
#1
Hafizah Y Chenia, Anelet Jacobs
Antibacterial compounds and metals co-select for antimicrobial resistance when bacteria harbour resistance genes towards both types of compounds, facilitating the proliferation and evolution of antimicrobial and heavy metal resistance. Antimicrobial and heavy metal resistance indices of 42 Gram-negative bacteria from a tilapia aquaculture system were determined to identify possible correlations between these phenotypes. Agar dilution assays were carried out to determine susceptibility to cadmium, copper, lead, mercury, chromate and zinc, while susceptibility to 21 antimicrobial agents was investigated by disk diffusion assays...
November 21, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29151459/the-ball-on-disk-cyclic-wear-of-cad-cam-machinable-dental-composite-and-ceramic-materials
#2
Kazuyuki Handa, Natsuko Murakami, Toshiki Yamazaki, Hidekazu Takahashi, Noriyuki Wakabayashi
The purpose of this study was to investigate the wear volume and the principal strain of machinable dental composite and ceramics in simulated mastication. A ball-on-disk wear test was performed for 3,000 cycles in water, using nine ball/disk combinations of three commercial CAD/CAM materials: feldspathic, lithium disilicate glass ceramics, and a highly loaded composite material (n = 7 for each combination). The wear volume was optically measured using a digital scanner and analyzed for statistical differences based on the materials (α = 0...
November 17, 2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29144958/isolation-of-blandm-producing-enterobacteriaceae-in-a-public-hospital-in-salvador-bahia-brazil
#3
Maria Goreth Barberino, Silvia de Araujo Cruvinel, Célio Faria, Marco Aurélio Salvino, Márcio Oliveira
Carbapenemases have great importance in the global epidemiological scenario since infections with carbapenemase-producing bacteria are associated with high mortality, especially in hospitalized patients in intensive care units. This study describes two microorganisms producers of the New Delhi Metallo-b-lactamase (NDM), Klebsiella pneumoniae and Citrobacter freundii, from two patients admitted to a public hospital in Salvador, Bahia. These are the first clinical cases of NDM described in microorganisms in the north and northeast Brazil...
November 13, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29138665/-epidemiological-profile-of-uropathogenic-enterobacteria-producing-extended-spectrum-beta-lactamases
#4
Mohammed Sbiti, Khalid Lahmadi, Lhoussaine Louzi
Urinary tract infections due to enterobacteria producing extended spectrum beta-lactamases (ESBL-E) constitute a infectious risk, a major therapeutic challenge and can even lead, in some cases, to a deadlock beacuse of their multi-resistance to antibiotics. This study aimed to determine the epidemiological profile of uropathogenic ESBL-E and to describe their current level of resistance to antibiotics for a better patient management according to local data. We conducted a retrospective study of all ESBL-E strains isolated from all cytobacteriogical testing of urine (CBEU) treated in the Microbiology Laboratory at the Military Hospital of Moulay Ismail, Meknes over a period of three years (from 1 January 2013 to 31 December 2015)...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29138101/primary-antibiotic-resistance-of-helicobacter-pylori-in-chinese-patients-a-multi-region-prospective-7-year-study
#5
Dong-Sheng Liu, You-Hua Wang, Zhi-Rong Zeng, Zhen-Yu Zhang, Hong Lu, Jian-Ming Xu, Yi-Qi Du, Yan Li, Jiang-Bin Wang, San-Ping Xu, Ye Chen, Chun-Hui Lan, Hong Cheng, Ming-de Jiang, Ling-Xia Zhang, Li-Juan Huo, Shi-Yao Chen, Guo-Xin Zhang, Kai-Chun Wu, Xuan Zhu, You-Xiang Chen, Yin Zhu, Xu Shu, Yong Xie, Nong-Hua Lu
OBJECTIVES: To explore the characteristics of H. pylori resistance in China and the association between antibiotic resistance and several clinical factors. METHODS: H. pylori strains were collected from patients in 13 provinces/cities in China between 2010 and 2016. Demographic data including type of disease, geographic area, age, gender and isolation year were collected to analyse their association with antibiotic resistance. Antibiotic resistance was detected using the Epsilometer test and the Kirby-Bauer disk diffusion method...
November 11, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29137595/study-of-bacterial-infections-among-patients-receiving-kidney-transplant-in-mashhad-iran
#6
Davood Mansury, Azad Khaledi, Kiarash Ghazvini, Mahin Ghorban Sabbagh, Hosna Zare, Mohammad Hossein Rokni-Hosseini, Hossein Vazini
OBJECTIVES: Over the past 2 decades, significant advances have been made in the management of infections after transplant; however, transplant recipients are still at high risk of infectious complications. This study aimed to evaluate the prevalence of bacterial infections and antimicrobial resistance patterns in kidney transplant recipients. MATERIALS AND METHODS: This cross-sectional study included 356 patients who received kidney transplants, regardless of the underlying disease, from 2013 to 2015 at the Montaserieh Transplant Hospital (Mashhad, Iran)...
November 15, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29137487/emergence-of-fosfomycin-resistance-among-isolates-of-escherichia-coli-harboring-extended-spectrum-and-ampc-%C3%AE-lactamases
#7
Aghil Bahramian, Gita Eslami, Ali Hashemi, Ali Tabibi, Mohsen Heidary
Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of fosA3 and fosC2 genes in extended-spectrum β-lactamases (ESBL) and blaDHA, blaCMY-2, and blaCMY-42 genes in AmpC β-lactamases-producing isolates of Escherichia coli. In total, 120 isolates of E. coli were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of blaCMY-2, blaCMY-42, blaDHA, fosA3, and fosC2 genes was detected by polymerase chain reaction (PCR) and sequencing...
November 15, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/29135450/influence-of-acquisition-frame-rate-and-video-compression-techniques-on-pulse-rate-variability-estimation-from-vppg-signal
#8
Luca Cerina, Luca Iozzia, Luca Mainardi
In this paper, common time- and frequency-domain variability indexes obtained by pulse rate variability (PRV) series extracted from video-photoplethysmographic signal (vPPG) were compared with heart rate variability (HRV) parameters calculated from synchronized ECG signals. The dual focus of this study was to analyze the effect of different video acquisition frame-rates starting from 60 frames-per-second (fps) down to 7.5 fps and different video compression techniques using both lossless and lossy codecs on PRV parameters estimation...
November 14, 2017: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/29134587/purification-of-bacteriophages-using-anion-exchange-chromatography
#9
Dieter Vandenheuvel, Sofie Rombouts, Evelien M Adriaenssens
In bacteriophage research and therapy, most applications ask for highly purified phage suspensions. The standard technique for this is ultracentrifugation using cesium chloride gradients. This technique is cumbersome, elaborate and expensive. Moreover, it is unsuitable for the purification of large quantities of phage suspensions.The protocol described here, uses anion-exchange chromatography to bind phages to a stationary phase. This is done using an FLPC system, combined with Convective Interaction Media (CIM(®)) monoliths...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29133211/beta-lactam-resistance-among-enterobacteriaceae-in-cambodia-the-four-year-itch
#10
REVIEW
Yannick Caron, Rattanak Chheang, Puthea Nop, Soda Meng, Sébastien Boyer, Arnaud Tarantola, Alexandra Kerléguer
Although antibiotics are too often used inappropriately in Cambodia, few data have been published on antimicrobial resistance in this country. Epidemic dissemination and the transfer of resistance genes to other bacterial species put the population at risk. The aim of this study was to evaluate the frequency and characteristics of extended-spectrum beta-lactamase (ESBL) enterobacteriaceae (ESBL-E) isolated in consecutive samples tested at Institut Pasteur du Cambodge (IPC) over a four-year period (2012-2015)...
November 10, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29130615/in-office-bleaching-efficacy-on-stain-removal-from-cad-cam-and-direct-resin-composite-materials
#11
Amal Alharbi, Stefano Ardu, Tissiana Bortolotto, Ivo Krejci
OBJECTIVE: To evaluate the efficacy of in-office bleaching on stain removal from stained resin composite and ceramic computer-assisted design/computer-assisted manufacturing (CAD/CAM) blocks and direct resin composites. METHODS: Forty disk-shaped samples were fabricated from each of nine materials: six CAD/CAM (VITABLOCS Mark II, Paradigm MZ100, Exp Vita Hybrid Ceramic, VITA ENAMIC, Exp Kerr, and LAVA Ultimate) and three direct resin composites (Filtek Supreme, Venus Diamond, and Filtek Silorane)...
November 11, 2017: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/29120259/signal-alteration-in-the-optic-nerve-head-on-3d-t2-weighted-mri-a-potential-neuroimaging-sign-of-glaucomatous-optic-neuropathy
#12
Jong Yeon Lee, Hyo Jeong Kwon, Su Jin Park, Chungkwon Yoo, Yong Yeon Kim, Eung Yeop Kim
PURPOSE: To investigate whether a signal alteration (SA) in the optic nerve head (ONH) on 3D T2-weighted magnetic resonance imaging (MRI) is associated with glaucomatous optic neuropathy Materials and Methods: A total of 35 patients with bilateral open-angle glaucoma and 31 age-matched controls underwent 3D high-resolution (0.98 × 0.98 × 1 mm(3)) T2-weighted MRI and detailed ophthalmologic examinations including spectral-domain optical coherence tomography (OCT). Two independent reviewers blinded to subject data determined mild or prominent SA on 3D T2-weighted MRI for the incomplete or complete loss of ocular hypointense continuity in the ONH, respectively...
November 9, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29118410/the-microbiological-characteristics-and-risk-factors-for-picc-related-bloodstream-infections-in-intensive-care-unit
#13
Shumin Zhang, Xiaofeng Sun, Yan Lei
The study was aimed to investigate the pathogens distribution and risk factors for PICC-related bloodstream infection in intensive care unit (ICU) patients. 402 patients placed with PICC in ICU were recruited in the study. The microbiological characteristics of PICC-related infection were investigated by Vitek 2 Compact automated microbial system. Antibiotics sensitivity was performed with disk diffusion and minimum inhibitory concentration (MIC) methods. Multivariate logistic and cox analyses were performed to identify the risk factors for PICC-related infection in ICU patients...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117078/prevalence-antimicrobial-susceptibility-and-clonal-diversity-of-pseudomonas-aeruginosa-in-chronic-wounds
#14
Fernanda Pessanha de Oliveira, Bruna Maiara Ferreira Barreto Pires, Keila de Cássia Ferreira de Almeida Silva, Bernadete Teixeira Ferreira de Carvalho, Lenise Arneiro Teixeira, Geraldo Renato de Paula, Beatriz Guitton Renaud Baptista de Oliveira
PURPOSE: Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility of P. aeruginosa strains to various antimicrobials, (3) detect the presence of virulence factors exoenzyme S (exoS) and exoenzyme U (exoU) in P. aeruginosa strains, and (4) evaluate wound colonization by P. aeruginosa via pulsed-field gel electrophoresis (PFGE). DESIGN: Descriptive research using a quantitative approach...
November 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/29112898/development-and-validation-of-an-analytical-method-using-uplc-ms-ms-to-quantify-everolimus-in-dried-blood-spots-in-the-oncology-setting
#15
Lotte M Knapen, Yvo de Beer, Roger J M Brüggemann, Leo M Stolk, Frank de Vries, Vivianne C G Tjan-Heijnen, Nielka P van Erp, Sander Croes
While the therapeutic drug monitoring (TDM) of everolimus has been routinely performed for over 10 years in solid organ transplantation medicine, in order to optimize the balance between effectiveness and toxicity, it is yet uncommon in the treatment of malignancies. The aim of this study was to develop and validate a bioanalytical method to quantify everolimus in dried blood spots (DBS) to facilitate TDM for the oncology outpatient setting. The hematocrit effect of everolimus was investigated. An 7.5mm disk from the central part of the DBS was punched, followed by the extraction of everolimus from the DBS by methanol/acetonitrile (80/20%) spiked with deuterium-labelled everolimus as internal standard...
October 29, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29111412/serotype-distribution-and-antibiotic-susceptibility-of-streptococcus-pneumoniae-strains-in-the-south-of-tunisia-a-five-year-study-2012-2016-of-pediatric-and-adult-populations
#16
Sonia Ktari, Ikram Jmal, Manel Mroua, Sonda Maalej, Nour ElHouda Ben Ayed, Basma Mnif, Faouzia Rhimi, Adnene Hammami
OBJECTIVES: To analyze the serotype distribution of Streptococcus pneumoniae clinical isolates collected in the south of Tunisia over a 5-year period in different age groups and to assess their antimicrobial susceptibility patterns. METHODS: A total of 305 non-duplicate S. pneumoniae isolates were collected between January 2012 and December 2016 at the university hospital in Sfax, Tunisia. All isolates were serotyped by multiplex PCR. The antibiotic susceptibility of all isolates was determined using the disk diffusion test or Etest assay...
October 27, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29110460/illustrating-the-mass-transport-effect-on-enzyme-cascade-reaction-kinetics-by-use-of-a-rotating-ring-disk-electrode
#17
Zeng-Qiang Wu, Jun-Jun Liu, Jin-Yi Li, Dan Xu, Xing-Hua Xia
Electrochemical biosensors based on enzymatic reaction have been applied to a wide range of fields. As the trend continues to grow, these biosensors are approaching the limit imposed by physics and chemistry. To further improve the performance of biosensors, the interplay of mass transport and enzymatic reaction kinetics, especially in enzyme cascade systems, should be considered in the design of biosensors. Herein, we propose a simple approach to studying the influence of mass transport and enzyme molecule motion on the kinetics of enzyme cascade reactions...
November 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29109545/the-lunar-core-can-be-a-major-reservoir-for-volatile-elements-s-se-te-and-sb
#18
Edgar S Steenstra, Yanhao Lin, Dian Dankers, Nachiketa Rai, Jasper Berndt, Sergei Matveev, Wim van Westrenen
The Moon bears a striking compositional and isotopic resemblance to the bulk silicate Earth (BSE) for many elements, but is considered highly depleted in many volatile elements compared to BSE due to high-temperature volatile loss from Moon-forming materials in the Moon-forming giant impact and/or due to evaporative loss during subsequent magmatism on the Moon. Here, we use high-pressure metal-silicate partitioning experiments to show that the observed low concentrations of volatile elements sulfur (S), selenium (Se), tellurium (Te), and antimony (Sb) in the silicate Moon can instead reflect core-mantle equilibration in a largely to fully molten Moon...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29104714/a-hybrid-task-graph-scheduler-for-high-performance-image-processing-workflows
#19
Timothy Blattner, Walid Keyrouz, Shuvra S Bhattacharyya, Milton Halem, Mary Brady
Designing applications for scalability is key to improving their performance in hybrid and cluster computing. Scheduling code to utilize parallelism is difficult, particularly when dealing with data dependencies, memory management, data motion, and processor occupancy. The Hybrid Task Graph Scheduler (HTGS) improves programmer productivity when implementing hybrid workflows for multi-core and multi-GPU systems. The Hybrid Task Graph Scheduler (HTGS) is an abstract execution model, framework, and API that increases programmer productivity when implementing hybrid workflows for such systems...
December 2017: Journal of Signal Processing Systems
https://www.readbyqxmd.com/read/29102052/reduction-of-bacterial-burden-by-copper-alloys-on-high-touch-athletic-center-surfaces
#20
Zina Ibrahim, Alexandra J Petrusan, Patrick Hooke, Shannon M Hinsa-Leasure
BACKGROUND: Athletic centers have been locations for the transmission of community-acquired infections. This study assessed the capacity of copper alloys to reduce the bacterial burden associated with high-touch athletic center equipment. Copper alloy weights and grips were rotated with rubber coated and stainless steel controls in an undergraduate college athletic center over a 16-month period. The athletic center is used by college athletic teams, student body, and local community. METHODS: The primary outcome was to compare bacterial burdens on copper and control grips by swabbing surfaces...
October 6, 2017: American Journal of Infection Control
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