keyword
MENU ▼
Read by QxMD icon Read
search

high Disk

keyword
https://www.readbyqxmd.com/read/28528638/triclem-combining-high-precision-room-temperature-clem-with%C3%A2-cryo-fluorescence-microscopy-to-identify-very-rare-events
#1
Nicholas R Ader, Wanda Kukulski
Fiducial-based correlation of fluorescence and electron microscopy data from high-pressure frozen and resin-embedded samples allows for high-precision localization of fluorescent signals to subcellular ultrastructure. Here we introduce the triCLEM procedure to facilitate the identification of very rare events for high-precision correlation. We present a detailed protocol to screen high-pressure frozen cell monolayers on sapphire disks for very rare signals by cryo-fluorescence microscopy, relocate the cells of interest after freeze substitution and Lowicryl embedding, and perform fiducial-based correlation of the identified fluorescent signals to high-magnification electron tomograms...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28525476/magnetic-resonance-classification-system-of-cervical-intervertebral-disk-degeneration-its-validity-and-meaning
#2
Akinobu Suzuki, Michael D Daubs, Tetsuo Hayashi, Monchai Ruangchainikom, Chenjie Xiong, Kevin Phan, Trevor P Scott, Jeffery C Wang
STUDY DESIGN: Retrospective analysis of kinetic magnetic resonance images (kMRIs). OBJECTIVE: (1) To analyze the changes seen on MRI related to disk degeneration and to develop a new grading system for cervical disk degeneration. (2) To evaluate the reliability and validity of the grading system. SUMMARY OF BACKGROUND DATA: Few have studied the relationship between changes seen on MRI with cervical disk degeneration and the chronological order of disk degeneration...
June 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28521744/determination-of-the-sources-and-antimicrobial-resistance-patterns-of-salmonella-isolated-from-the-poultry-industry-in-southern-ethiopia
#3
Reta Duguma Abdi, Fisseha Mengstie, Ashenafi Feyisa Beyi, Takele Beyene, Hika Waktole, Bedasso Mammo, Dinka Ayana, Fufa Abunna
BACKGROUND: Ethiopia set an ambitious masterplan to increase chicken meat and egg production from 2015 to 2020. Poultry breeding, multiplication and distribution centers in the country have received executive order to import, amplify and distribute commercial chickens to end users. The biosecurity and the pathogen fauna of the centers have not been evaluated as to whether the centers could implement the mission effectively without any risk. Thus, the aim of this study was to evaluate the biosecurity practices and the pathogen prevalence, risk factors and their antimicrobial resistance (AMR) using Salmonella as case study...
May 18, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28520443/effective-interactions-and-colloidal-stability-of-bovine-%C3%AE-globulin-in-solution
#4
Stefano Da Vela, Felix Roosen-Runge, Maximilian W A Skoda, Robert M J Jacobs, Tilo Seydel, Henrich Frielinghaus, Michael Sztucki, Ralf Schweins, Fajun Zhang, Frank Schreiber
Interactions and phase behavior of γ-globulins are of fundamental interest in biophysical and pharmaceutical research, as these are among the most abundant proteins in blood plasma. In this work, we report the characterization of the oligomeric state of bovine γ-globulin, the effective protein-protein interactions and the colloidal stability in aqueous solution as a function of protein concentration and ionic strength. Classical biochemical techniques, such as size exclusion chromatography (SEC) and gel electrophoresis together with small angle X-ray and neutron scattering (SAXS/SANS) were employed for this study...
May 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28520428/cyclic-diarylheptanoids-from-corylus-avellana-green-leafy-covers-determination-of-their-absolute-configurations-and-evaluation-of-their-antioxidant-and-antimicrobial-activities
#5
Antonietta Cerulli, Gianluigi Lauro, Milena Masullo, Vincenza Cantone, Beata Olas, Bogdan Kontek, Filomena Nazzaro, Giuseppe Bifulco, Sonia Piacente
The methanol extract of the leafy covers of Corylus avellana, source of the Italian PGI (protected geographical indication) product "Nocciola di Giffoni", afforded two new cyclic diarylheptanoids, giffonins T and U (2 and 3), along with two known cyclic diarylheptanoids, a quinic acid, flavonoid-, and citric acid derivatives. The structures of giffonins T and U were determined as highly hydroxylated cyclic diarylheptanoids by 1D and 2D NMR experiments. Their relative configurations were assigned by a combined quantum mechanical/NMR approach, comparing the experimental (13)C/(1)H NMR chemical shift data and the related predicted values...
May 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28511383/study-on-mics-of-tigecycline-in-clinical-isolates-of-carbapenem-resistant-enterobacteriaceae-cre-at-a-tertiary-care-centre-in-north-india
#6
Vineeta Khare, Prashant Gupta, Fareya Haider, Rehana Begum
INTRODUCTION: Members of family Enterobacteriaceae are the most common Gram-negative bacteria isolated from clinical samples. Those Enterobacteriaceae which have acquired resistance to all β-lactams antibiotics including the carbapenems are considered as Carbapenem Resistant Enterobacteriaceae (CRE). These CRE isolates are often resistant to most other classes of antimicrobials as well, making their treatment a great challenge. Tigecycline is one of the last resort antimicrobials against such multidrug resistant bacteria...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28511382/rapid-identification-of-vancomycin-resistant-enterococcus-faecalis-clinical-isolates-using-a-sugar-fermentation-method
#7
Javad Raeisi, Mahnaz Saifi, Mohammad Reza Pourshafie, Mehri Habibi, Hamid Reza Mohajerani, Neda Akbari, Mohammad Reza Asadi Karam
INTRODUCTION: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. AIM: To measure the turanose fermentation in Enterococcus faecalis clinical isolates for rapid differentiation of VRE and Vancomycin-Susceptible E. faecalis (VSE) isolates. MATERIALS AND METHODS: Forty E. faecalis samples were isolated from 200 clinical samples in Tehran Medical Center, Iran, from October 2012 to December 2012...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28511287/generation-and-characterization-of-highly-purified-canine-schwann-cells-from-spinal-nerve-dorsal-roots-as-potential-new-candidates-for-transplantation-strategies
#8
Nicole Steffensen, Annika Lehmbecker, Ingo Gerhauser, Yimin Wang, Regina Carlson, Andrea Tipold, Wolfgang Baumgärtner, Veronika M Stein
Schwann cells (SCs) are promising candidates for transplantation-strategies in the central nervous system by promoting axonal regeneration. The dog represents a translational model for human spinal cord injury (SCI) for studies with new repair strategies after intervertebral disk herniation (IVDH). To overcome the necessity for an additional surgical procedure, for the first time a protocol for isolation, and purification of canine SCs from spinal-nerve-biopsies during standard hemilaminectomy in IVDH affected paraplegic dogs for potential transplantation was developed...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28511165/susceptibility-of-streptococcus-pneumoniae-to-fluoroquinolones-and-macrolides-in-upper-respiratory-tract-infections
#9
Yaroslav O Mykhalko, Tetyana V Duhovych, Pavlo P Kish
INTRODUCTION: Streptococcal species are known as the most common cause of bacterial upper respiratory tract infections (URTI). Once bacterial infection is diagnosed it demands empirical antibiotic prescription. On the other hand antimicrobial resistance is a global burden in today's medicine. For that reason, knowing of antimicrobial susceptibility patterns in population is an important background for successful treatment of bacterial caused URTI. The aim of this study was to analyze S...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28506250/antimicrobial-resistance-profile-of-staphylococcus-aureus-isolates-isolated-from-ear-discharges-of-patients-at-university-of-hawassa-comprehensive-specialized-hospital
#10
Serawit Deyno, Alemayehu Toma, Mesfin Worku, Muluken Bekele
BACKGROUND: Drug-resistant microorganism are a growing global danger. Strains of S. aureus have developed resistance to many commonly used antimicrobials due to indiscriminate use of antimicrobials, and treatment becoming a challenge. Studies assessing pattern and determinants of S. aureus resistance in ear infection in Ethiopia are very scarce. This study presents overview of pattern and determinants S. aureus resistance from samples of ear discharge in Ethiopia. METHOD: A prospective cross-sectional study was conducted on patients who visit ENT clinic of University of Hawassa comprehensive specialized hospital from February 1, 2016 to November 1, 2016...
May 15, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28505834/dynamic-phases-clustering-and-chain-formation-for-driven-disk-systems-in-the-presence-of-quenched-disorder
#11
Y Yang, D McDermott, C J Olson Reichhardt, C Reichhardt
We numerically examine the dynamic phases and pattern formation of two-dimensional monodisperse repulsive disks driven over random quenched disorder. We show that there is a series of distinct dynamic regimes as a function of increasing drive, including a clogged or pile-up phase near depinning, a homogeneous disordered flow state, and a dynamically phase separated regime consisting of high-density crystalline regions surrounded by a low density of disordered disks. At the highest drives the disks arrange into one-dimensional moving chains...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28505803/simulating-competitive-egress-of-noncircular-pedestrians
#12
R C Hidalgo, D R Parisi, I Zuriguel
We present a numerical framework to simulate pedestrian dynamics in highly competitive conditions by means of a force-based model implemented with spherocylindrical particles instead of the traditional, symmetric disks. This modification of the individuals' shape allows one to naturally reproduce recent experimental findings of room evacuations through narrow doors in situations where the contact pressure among the pedestrians was rather large. In particular, we obtain a power-law tail distribution of the time lapses between the passage of consecutive individuals...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28505094/antigiardial-activity-of-podophyllotoxin-type-lignans-from-bursera-fagaroides-var-fagaroides
#13
Filiberto Gutiérrez-Gutiérrez, Ana María Puebla-Pérez, Sirenia González-Pozos, José Manuel Hernández-Hernández, Armando Pérez-Rangel, Laura Patricia Alvarez, Gabriela Tapia-Pastrana, Araceli Castillo-Romero
Giardiasis, a diarrheal disease, is highly prevalent in developing countries. Several drugs are available for the treatment of this parasitosis; unfortunately, all of them have variable efficacies and adverse effects. Bursera fagaroides has been known for its anti-inflammatory and antidiarrheal properties in Mexican traditional medicine. We investigated the in vitro anti-giardial activities of four podophyllotoxin-type lignans from Bursera fagaroides var. fagaroides, namely, 5'-desmethoxy-β-peltatin-A-methylether (5-DES), acetylpodophyllotoxin (APOD), burseranin (BUR), and podophyllotoxin (POD)...
May 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28504940/good-random-multi-triangulation-of-surfaces
#14
Esdras de Medeiros Filho, Marcelo Siqueira
We introduce the Hierarchical Poisson Disk Sampling Multi-Triangulation (HPDS-MT) of surfaces, a novel structure that combines the power of multi-triangulation (MT) with the benefits of Hierarchical Poisson Disk Sampling (HPDS). MT is a general framework for representing surfaces through variable resolution triangle meshes, while HPDS is a well-spaced random distribution with blue noise characteristics. The distinguishing feature of the HPDS-MT is its ability to extract adaptive meshes whose triangles are guaranteed to have good shape quality...
May 12, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/28502972/distribution-innervation-and-cellular-organization-of-taste-buds-in-the-sea-catfish-plotosus-japonicus
#15
Tatsufumi Nakamura, Naoki Matsuyama, Masato Kirino, Masanori Kasai, Sadao Kiyohara, Takanori Ikenaga
The gustatory system of the sea catfish Plotosus japonicus, like that of other catfishes, is highly developed. To clarify the details of the morphology of the peripheral gustatory system of Plotosus, we used whole-mount immunohistochemistry to investigate the distribution and innervation of the taste buds within multiple organs including the barbels, oropharyngeal cavity, fins (pectoral, dorsal, and caudal), and trunk. Labeled taste buds could be observed in all the organs examined. The density of the taste buds was higher along the leading edges of the barbels and fins; this likely increases the chance of detecting food...
May 13, 2017: Brain, Behavior and Evolution
https://www.readbyqxmd.com/read/28501480/assessing-manual-dexterity-comparing-the-workability-rate-of-manipulation-test-with-the-minnesota-manual-dexterity-test
#16
Ying-Chih Wang, Rick Wickstrom, Sheng-Che Yen, Jay Kapellusch, Kimberly A Grogan
STUDY DESIGN: Cross-sectional study. INTRODUCTION: The WorkAbility Rate of Manipulation Test (WRMT), an adaptation of the Minnesota Manual Dexterity Test (MMDT), contains a revised board and protocols to improve its utility for therapy or fitness assessment. PURPOSE OF THE STUDY: To describe the development and preliminary psychometric properties of WRMT. METHODS: Sixty-six healthy participants completed MMDT and WRMT in a random order followed by a user experience survey...
May 10, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/28495879/dual-comb-spectroscopy-of-water-vapor-with-a-free-running-semiconductor-disk-laser
#17
S M Link, D J H C Maas, D Waldburger, U Keller
Dual-comb spectroscopy offers the potential for high accuracy combined with fast data acquisition. Applications are often limited, however, by the complexity of optical comb systems. Here we present dual-comb spectroscopy of water vapor using a substantially simplified single-laser system. Very good spectroscopy measurements with fast sampling rates are achieved with a free-running dual-comb mode-locked semiconductor disk laser. The absolute stability of the optical comb modes is characterized both for free-running operation and with simple microwave stabilization...
May 11, 2017: Science
https://www.readbyqxmd.com/read/28494422/cyanobacteria-blooms-before-and-during-the-restoration-process-of-a-shallow-urban-lake
#18
Joanna Rosińska, Anna Kozak, Renata Dondajewska, Ryszard Gołdyn
Swarzędzkie Lake (near Poznań) has been heavily polluted. To improve the water quality, the restoration of lake by three methods: aeration, phosphorus inactivation using small doses of iron sulphate and magnesium chloride (FeSO4 and MgCl2) and biomanipulation was initiated at the end of 2011. The aim of the present study was to determine whether sustainable restoration has a significant impact on phytoplankton, especially cyanobacterial blooms in a shallow, urban, degraded lake. Therefore, phytoplankton and the physico-chemical parameters of water at the summer thermal stratification and autumn water mixing before (2011) and during restoration (2012-2014) was studied...
May 7, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28493889/characterization-of-a-novel-antibiofilm-effect-of-nitric-oxide-releasing-aspirin-ncx-4040-on-candida-albicans-isolates-from-denture-stomatitis-patients
#19
Francisco Madariaga-Venegas, Roberto Fernández-Soto, Luisa Fernanda Duarte, Nicole Suarez, Daniela Delgadillo, José A Jara, Ricardo Fernández-Ramires, Blanca Urzúa, Alfredo Molina-Berríos
Candida albicans biofilms play a key role in denture stomatitis, one of the most common oral pathologies in elderly people. Because biofilms are highly resistant to antifungals, new pharmacological strategies are needed. Aspirin and nitric oxide-donor molecules have both shown antibiofilm effects on C. albicans, making them promising candidates for treatment. In this study, we evaluated the antifungal/antibiofilm effect of a nitric-oxide releasing aspirin (NO-ASA) on C. albicans isolates from denture stomatitis patients in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28491203/traumatic-lumbar-vertebral-ring-apophysis-fracture-with-disk-herniation-in-an-adolescent
#20
Geetanjalee Kadam, Kaustubh Narsinghpura, Sonali Deshmukh, Sanjay Desai
We present a case of a 15-year-old male with history of back pain and bilateral lower limb radiculopathy due to fall. The magnetic resonance imaging scan showed disc bulge at L2-L3 level causing compression on contained nerve roots. In this case, computed tomography scan was indispensable for diagnosis and classification of the vertebral apophyseal fracture and to guide appropriate further management. Apophyseal ring fracture is an uncommon cause of back pain with radiculopathy in adolescents and athletes. High degree of suspicion is necessary to differentiate these injuries from disc herniation so as to further guide appropriate conservative or surgical management...
June 2017: Radiology case reports
keyword
keyword
60783
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"