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https://www.readbyqxmd.com/read/29772868/-significance-of-cell-autophagy-in-resorption-of-lumbar-disc-herniation
#1
REVIEW
Zhi-Jun Diao, Hong Jiang, Jin-Tao Liu
As a self-protective mechanism of cells, autophagy of cells can maintain cell stability by degrading self-aging substances, and it can be highly induced. The ability of autophagy to degraded cells will decrease with age. The resorption phenomenon after lumbar disc herniation is one of the effective mechanisms in conservative treatment of lumbar disc herniation. The degenerative lesion of intervertebral disc is one of the main reasons of lumbar disc herniation. Cell autophagy is extensive participation in the degeneration of lumbar intervertebral disc, delaying the occurrence of degenerative disease...
April 25, 2018: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29768222/green-synthesis-of-silver-nanoparticles-using-flower-extract-of-malva-sylvestris-and-investigation-of-their-antibacterial-activity
#2
Hassan Mahmoodi Esfanddarani, Abolghasem Abbasi Kajani, Abdol-Khalegh Bordbar
High-quality colloidal silver nanoparticles (AgNP) were synthesised via a green approach by using hydroalcoholic extracts of Malva sylvestris . Silver nitrate was used as a substrate ion while the plant extract successfully played the role of reducing and stabilising agents. The synthesised nanoparticles were carefully characterised by using transmission electron microscopy, atomic-force microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and UV-vis spectroscopy. The maximum absorption wavelengths of the colloidal solutions synthesised using 70 and 96% ethanol and 100% methanol, as extraction solvents, were 430, 485 and 504 nm, respectively...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29765579/antagonistic-effect-of-isolated-probiotic-bacteria-from-natural-sources-against-intestinal-escherichia-coli-pathotypes
#3
Sahar Karimi, Ehsan Rashidian, Mehdi Birjandi, Leila Mahmoodnia
Background: Probiotics are live microorganisms which are beneficial bacteria that are normal flora of the digestive system which, in determined amounts, show beneficial effects on host health, and can balance gastrointestinal microflora. Digestive tract diseases such as diarrhea are one of the major causes of child mortality in developing countries. Different pathotypes of Escherichia coli cause diarrhea that affects children, therefore reduction of these colonization strains in humans or animals can decline gastrointestinal disorders such as diarrhea...
March 2018: Electronic Physician
https://www.readbyqxmd.com/read/29765111/new-insights-into-apoptosome-structure-and-function
#4
REVIEW
Loretta Dorstyn, Christopher W Akey, Sharad Kumar
The apoptosome is a platform that activates apical procaspases in response to intrinsic cell death signals. Biochemical and structural studies in the past two decades have extended our understanding of apoptosome composition and structure, while illuminating the requirements for initiator procaspase activation. A number of studies have now provided high-resolution structures for apoptosomes from C. elegans (CED-4), D. melanogaster (Dark), and H. sapiens (Apaf-1), which define critical protein interfaces, including intra and interdomain interactions...
May 15, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29762496/arc-discharges-of-a-pure-carbon-strip-affected-by-dynamic-contact-force-during-current-carrying-sliding
#5
Yanyan Zhang, Yongzhen Zhang, Chenfei Song
Arc discharges of a pure carbon strip induced by dynamic contact force were studied on a pin-on-disk tribometer. It was found that arc discharges were produced periodically in accordance with the period of the dynamic contact force. The arcing rate of the pure carbon strip increased with an increase of frequency f and amplitude B , which led to a decrease of current-carrying quality. These influences at high velocities became much more significant. A critical point of the arcing rate at around 2% was detected...
May 15, 2018: Materials
https://www.readbyqxmd.com/read/29759936/in-vitro-antioxidant-antilipoxygenase-and-antimicrobial-activities-of-extracts-from-seven-climbing-plants-belonging-to-the-bignoniaceae
#6
Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez
OBJECTIVES: This study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. These species are Adenocalymma marginatum (Cham.) DC., Amphilophium vauthieri DC., Cuspidaria convoluta (Vell.) A. H. Gentry, Dolichandra dentata (K. Schum.) L. G. Lohmann, Fridericia caudigera (S. Moore) L...
May 3, 2018: Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29758266/alanine-rich-peptide-from-populus-trichocarpa-inhibit-growth-of-staphylococcus-aureus-via-targetting-its-extracellular-domain-of-sensor-histidine-kinase-yycgex-protein
#7
Raid Al Akeel, Ayesha Mateen, Rabbani Syed, Mohammed S Al-Qahtani, A Alqahtani
BACKGROUND: Due to growing concern towards microbial resistance, ongoing search for developing novel bioactive compounds such as peptides is on rise. The aim of this study was to evaluate antimicrobial effect of Populus trichocarpa extract, chemically identify the active peptide fraction and finds its target in Staphylococcus aureus. METHODS: In this study the active fraction of P. trichocarpa crude extract was purified and characterized using MS/MS. This peptide PT13 antimicrobial activity was confirmed by in-vitro agar based disk diffusion and in-vivo infection model of G...
May 11, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29756621/electrochemical-oxidation-of-ciprofloxacin-in-two-different-processes-the-electron-transfer-process-on-the-anode-surface-and-the-indirect-oxidation-process-in-bulk-solutions
#8
Bo Shen, Xianghua Wen, Gregory V Korshin
Herein, the rotating disk electrode technique was used for the first time to investigate the effects of mass-transfer limitations and pH on the electrochemical oxidation of CPX, to determine the kinetics of CPX oxidation and to explore intrinsic mechanisms during the electron transfer process. Firstly, cyclic voltammetry revealed that an obvious irreversible CPX oxidation peak was observed within the potential window from 0.70 to 1.30 V at all pHs. Based on the Levich equation, the electrochemical oxidation of CPX in the electron transfer process was found to be controlled by both diffusion and kinetic processes when pH = 2, 5, 7 and 9; the diffusion coefficient of CPX at pH = 2 was calculated to be 1...
May 14, 2018: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/29751593/comet-pond-ii-synergistic-intersection-of-concentrated-extraterrestrial-materials-and-planetary-environments-to-form-procreative-darwinian-ponds
#9
Benton C Clark, Vera M Kolb
In the “comet pond” model, a rare combination of circumstances enables the entry and landing of pristine organic material onto a planetary surface with the creation of a pond by a soft impact and melting of entrained ices. Formation of the constituents of the comet in the cold interstellar medium and our circumstellar disk results in multiple constituents at disequilibrium which undergo rapid chemical reactions in the warmer, liquid environment. The planetary surface also provides minerals and atmospheric gases which chemically interact with the pond’s organic- and trace-element-rich constituents...
May 11, 2018: Life
https://www.readbyqxmd.com/read/29748509/high-speed-lateral-flow-strategy-for-a-fast-biosensing-with-an-improved-selectivity-and-binding-affinity
#10
Dong Guk Cho, Haneul Yoo, Haein Lee, Yeol Kyo Choi, Minju Lee, Dong June Ahn, Seunghun Hong
We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors...
May 10, 2018: Sensors
https://www.readbyqxmd.com/read/29746496/pharyngeal-colonization-and-drug-resistance-profiles-of-morraxella-catarrrhalis-streptococcus-pneumoniae-staphylococcus-aureus-and-haemophilus-influenzae-among-hiv-infected-children-attending-art-clinic-of-felegehiwot-referral-hospital-ethiopia
#11
Wondemagegn Mulu, Endalew Yizengaw, Megbaru Alemu, Daniel Mekonnen, Derese Hailu, Kassaw Ketemaw, Bayeh Abera, Mulugeta Kibret
BACKGROUND: Asymptomatic pharyngeal colonization by potential bacteria is the primary reservoir for bacterial species within a population and is considered a prerequisite for development of major childhood diseases such as sinusitis, otitis media, pneumonia, bacteremia, and meningitis. However, there is dearth of data on the colonization and drug resistance pattern of the main bacterial pathogens in the pharynx of HIV infected children in Ethiopia. Therefore, this study determined the pharyngeal colonization and drug resistance profile of bacterial pathogens in HIV infected children attending ART clinic of Felegehiwot Referral Hospital (FHRH), Amhara Region, Ethiopia...
2018: PloS One
https://www.readbyqxmd.com/read/29746412/rapid-progression-of-foveomacular-retinoschisis-in-young-myopics
#12
Zhongcui Sun, Hua Gao, Min Wang, Qing Chang, Gezhi Xu
PURPOSE: To describe a foveomacular retinoschisis that has not been described. METHODS: Patients with foveomacular retinoschisis were included. Exclusion criteria included refractive error over -6.00 diopters, presence of posterior staphyloma, positive RS1-gene mutation, family history of retinoschisis, optic disk abnormalities, or glaucoma. Vitrectomy was performed on eyes with functional or structural deterioration. RESULTS: Seventeen eyes from 10 patients (15-30 years old, 8 females and 2 males) with foveoschisis were recruited, with bilateral involvement in 7 patients and unilateral in 3 patients...
May 2, 2018: Retina
https://www.readbyqxmd.com/read/29746162/antifungal-activity-of-essential-oils-against-azole-resistant-and-azole-susceptible-i-i-candida-glabrata-i-i-strains-vaginal-isolates
#13
Nadia Massa, Simone Cantamessa, Giorgia Novello, Elia Ranzato, Simona Martinotti, Matteo Pavan, Andrea Rocchetti, Graziella Berta, Elisa Gamalero, Elisa Bona
<i></i>Candida glabrata<i></i> is an opportunistic pathogen, associated with endocarditis, meningitis and disseminated disease and also with complicated vaginitis. Essential oils derived from aromatic plants are known in traditional medicine as antimicrobial agents and have antifungal properties. The aim of this work was to evaluate whether the twelve tested essential oils (tea tree, laurel, anise, basil, bergamot, lavender, mint, oregano, grapefruit, rosemary, winter savory and ginger) could have a transverse effect on <i></i>C...
May 10, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29745778/design-of-a-rotating-disk-reactor-to-assess-the-colonization-of-biofilms-by-free-living-amoebae-under-high-shear-rates
#14
A Perrin, P Herbelin, F P A Jorand, S Skali-Lami, L Mathieu
The present study was aimed at designing and optimizing a rotating disk reactor simulating high hydrodynamic shear rates (γ), which are representative of cooling circuits. The characteristics of the hydrodynamic conditions in the reactor and the complex approach used to engineer it are described. A 60 l tank was filled with freshwater containing free-living amoebae (FLA) and bacteria. Adhesion of the bacteria and formation of a biofilm on the stainless steel coupons were observed. FLA were able to establish in these biofilms under γ as high as 85,000 s-1 ...
May 10, 2018: Biofouling
https://www.readbyqxmd.com/read/29744453/studies-on-the-cytocompatibility-mechanical-and-antimicrobial-properties-of-3d-printed-poly-methyl-methacrylate-beads
#15
David K Mills, Uday Jammalamadaka, Karthik Tappa, Jeffery Weisman
Osteomyelitis is typically a bacterial infection (usually from Staphylococcus) or, more rarely, a fungal infection of the bone. It can occur in any bone in the body, but it most often affects the long bones (leg and arm), vertebral (spine), and bones of the foot. Microbial success in osteomyelitis is due to their ability to form biofilms which inhibit the wound healing process and increases resistance to anti-infective agents. Also, biofilms do not allow easy penetration of antibiotics into their matrix making clinical treatment a challenge...
June 2018: Bioactive Materials
https://www.readbyqxmd.com/read/29744322/dissemination-of-multidrug-resistant-class-i-and-ii-integrons-and-molecular-typing-of-ctx-m-producing-klebsiella-pneumoniae
#16
Alisha Akya, Azam Elahi, Roya Chegenelorestani, Mahya Rezaee
Introduction: Klebsiella pneumoniae ( K. pneumoniae ) is an important opportunistic pathogen causes serious community and hospital-acquired infections, which is highly resistant to antibiotics. We aimed to determine the frequency of multidrug resistant (MDR) and molecular typing of clinical isolates of K. pneumoniae . Methodology: One hundred isolates of K. pneumoniae were collected from clinical samples in three general hospitals in Kermanshah. The antimicrobial susceptibility and extended-spectrum beta-lactamases (ESBL) production of isolates were determined using disk diffusion and combined disk methods, respectively...
April 2018: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/29743305/five-year-national-surveillance-of-invasive-candidiasis-species-distribution-and-azole-susceptibility-from-the-china-hospital-invasive-fungal-surveillance-net-chif-net-study
#17
Meng Xiao, Zi-Yong Sun, Mei Kang, Da-Wen Guo, Kang Liao, Sharon C-A Chen, Fanrong Kong, Xin Fan, Jing-Wei Cheng, Xin Hou, Meng-Lan Zhou, Ying Li, Shu-Ying Yu, Jing-Jing Huang, He Wang, Ying-Chun Xu
Data on the epidemiology of invasive candidiasis (IC) and antifungal susceptibility of Candida isolates in China are still limited. Here we report surveillance for IC from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study. Sixty-five tertiary hospitals collected 8,829 Candida isolates from August 1, 2009 to July 31, 2014. Matrix-assisted laser desorption/ionization -time of flight mass spectrometry supplemented by rDNA sequencing was used to define species, and fluconazole and voriconazole susceptibilities determined by the Clinical and Laboratory Standards Institute disk diffusion method...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29741687/bowel-surgery-as-a-fertility-enhancing-procedure-in-patients-with-colorectal-endometriosis-methodological-pathogenic-and-ethical-issues
#18
Paolo Vercellini, Paola Viganò, Maria Pina Frattaruolo, Alessandra Borghi, Edgardo Somigliana
Bowel surgery for colorectal endometriosis is being promoted to infertile women without severe sub-occlusive symptoms, with the objective of improving the likelihood of conception. Contrary to rectal shaving, bowel surgery involving full-thickness disk excision and segmental resection entails opening of the intestinal lumen thus increasing the risk of postoperative infectious complications. About 1 in 10 patients undergoing colorectal resection for intestinal endometriosis will experience severe sequelae, including anastomotic dehiscence, rectovaginal fistula formation, and bladder and bowel denervation...
May 7, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29738506/macrophage-biocompatibility-of-cocr-wear-particles-produced-under-polarization-in-hyaluronic-acid-aqueous-solution
#19
Blanca Teresa Perez-Maceda, María Encarnación López-Fernández, Iván Díaz, Aaron Kavanaugh, Fabrizio Billi, María Lorenza Escudero, María Cristina García-Alonso, Rosa María Lozano
Macrophages are the main cells involved in inflammatory processes and in the primary response to debris derived from wear of implanted CoCr alloys. The biocompatibility of wear particles from a high carbon CoCr alloy produced under polarization in hyaluronic acid (HA) aqueous solution was evaluated in J774A.1 mouse macrophages cultures. Polarization was applied to mimic the electrical interactions observed in living tissues. Wear tests were performed in a pin-on-disk tribometer integrating an electrochemical cell in phosphate buffer solution (PBS) and in PBS supplemented with 3 g/L HA, an average concentration that is generally found in synovial fluid, used as lubricant solution...
May 8, 2018: Materials
https://www.readbyqxmd.com/read/29732321/characterization-of-non-typhoidal-salmonella-enterica-strains-of-human-origin-in-central-and-southern-italy
#20
Yolande T R Proroga, Federico Capuano, Rosanna Capparelli, Stefano Bilei, Mariano Bernardo, Maria Pia Cocco, Rosalba Campagnuolo, Vincenzo Pasquale
Non-typhoidal Salmonella enterica infection is a significant public health problem worldwide. The aim of this study was to characterize Salmonella enterica strains isolated from human specimens in central and southern Italy, for epidemiological studies. One hundred and fifty S. enterica strains were serotyped. Isolates were tested for their antimicrobial susceptibility, by disk diffusion method. The molecular characterizations, based on PCR, were carried out for the detection of invA gene and other virulence elements and phage marker genes...
March 31, 2018: Italian Journal of Food Safety
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