keyword
MENU ▼
Read by QxMD icon Read
search

TGF AND bone

keyword
https://www.readbyqxmd.com/read/29691795/improved-protocol-for-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-effect-of-pthrp-and-fgf-2-on-tgf%C3%AE-1-bmp2-induced-chondrocytes-hypertrophy
#1
Davood Nasrabadi, Siamak Rezaeiani, Mohamadreza Baghaban Eslaminejad, Aliakbar Shabani
Growth factors have a pivotal role in chondrogenic differentiation of stem cells. The differential effects of known growth factors involved in the maintenance and homeostasis of cartilage tissue have been previously studied in vitro. However, there are few reported researches about the interactional effects of growth factors on chondrogenic differentiation of stem cells. The aim of this study is to examine the combined effects of four key growth factors on chondrogenic differentiation of mesenchymal stem cells (MSCs)...
April 24, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29690697/-experimental-study-on-the-transforming-growth-factor-%C3%AE-3-combined-with-dental-pulp-stem-cells-in-early-bone-integration-of-implant
#2
Ababaikeli Guzalinuer, Huojia Muhetaer, H Wu, Abudureheman Paerhati
Objective: To establish the experimental model of rabbit mandibular anterior implant repair and evaluate the effects of transforming growth factor (TGF)-β3 and dental pulp stem cells (DPSC) in promoting the bone integration of implant. Methods: The New Zealand rabbits were randomly divided into experimental group, control group and blank group (6 rabbits for each group) . In the experimental group, the implant area was filled with the mixture of TGF-β3, DPSC and Bio-oss powder. In the control group, the implant area was filled with the mixture of DPSC and Bio-oss powder...
April 9, 2018: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/29688810/sustained-spatiotemporal-release-of-tgf-%C3%AE-1-confers-enhanced-very-early-chondrogenic-differentiation-during-osteochondral-repair-in-specific-topographic-patterns
#3
Ann-Kathrin Asen, Lars Goebel, Ana Rey-Rico, Jerome Sohier, David Zurakowski, Magali Cucchiarini, Henning Madry
The continuous presence of TGF-β is critically important to induce effective chondrogenesis. To investigate chondrogenesis in a cartilage defect, we tested the hypothesis that the implantation of TGF-β1-releasing scaffolds improves very early cartilage repair in vivo. Spatiotemporal controlled release of TGF-β1 was achieved from multiblock scaffolds that were implanted in osteochondral defects in the medial femoral condyles of adult minipigs. We observed a sustained presence of TGF-β1 at 4 wk in vivo, which significantly promoted structural aspects of early overall cartilage repair, especially cellularity, cellular morphology, and safranin O staining intensity...
April 24, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29687874/effects-of-platelet-rich-plasma-on-angiogenesis-and-osteogenesis-associated-factors-in-rabbits-with-avascular-necrosis-of-the-femoral-head
#4
X-L Zhang, K-Q Shi, P-T Jia, L-H Jiang, Y-H Liu, X Chen, Z-Y Zhou, Y-X Li, L-S Wang
OBJECTIVE: Platelet-rich plasma (PRP) contains various growth factors and cytokines that can enhance the recovery of the damaged tissues. The present study aimed to examine the effects of PRP on the recovery of avascular necrosis of the femoral head (ANFH), and to provide novel insights into the clinical treatment of this disease. MATERIALS AND METHODS: A total of 24 New Zealand white rabbits were randomly divided into the normal control group, ANFH model and PRP-treated groups (n =1 2 each)...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29682351/comparative-analysis-of-cellular-and-growth-factor-composition-in-bone-marrow-aspirate-concentrate-and-platelet-rich-plasma
#5
Hisashi Sugaya, Tomokazu Yoshioka, Toshiki Kato, Yu Taniguchi, Hiroshi Kumagai, Kojiro Hyodo, Osamu Ohneda, Masashi Yamazaki, Hajime Mishima
The purpose of this study was to quantify the stem cell and growth factor (GF) contents in the bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) prepared from whole blood using a protocol established in our laboratory. We examined 10 patients with osteonecrosis of the femoral head who were treated by autologous BMAC transplantation at our hospital between January 2015 and June 2015. We quantified CD34+ and CD31-CD45-CD90+CD105+ cells in BMAC and PRP by flow cytometry. Additionally, we measured various GFs, that is, basic fibroblast growth factor (b-FGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), transforming growth factor- β 1 (TGF- β 1), and bone morphogenetic protein-2 (BMP-2) in BMAC and PRP using enzyme-linked immunosorbent assays and statistical analyses...
2018: Bone Marrow Research
https://www.readbyqxmd.com/read/29682160/transplantation-of-bone-marrow-mesenchymal-stem-cells-prevents-radiation-induced-artery-injury-by-suppressing-oxidative-stress-and-inflammation
#6
Yanjun Shen, Xin Jiang, Lingbin Meng, Chengcheng Xia, Lihong Zhang, Ying Xin
The present study aims to explore the protective effect of human bone marrow mesenchymal stem cells (hBMSCs) on radiation-induced aortic injury (RIAI). hBMSCs were isolated and cultured from human bone marrow. Male C57/BL mice were irradiated with a dose of 18-Gy 6MV X-ray and randomly treated with either vehicle or hBMSCs through tail vein injection with a dose of 103 or 104 cells/g of body weight (low or high dose of hBMSCs) within 24 h. Aortic inflammation, oxidative stress, and vascular remodeling were assessed by immunohistochemical staining at 3, 7, 14, 28, and 84 days after irradiation...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29680988/tgf-%C3%AE-negatively-regulates-mitf-e-expression-and-canine-osteoclastogenesis
#7
Kumiko Asai, Masaharu Hisasue, Fumie Shimokawa, Masayuki Funaba, Masaru Murakami
With longevity, the prevalence of osteoporosis, which occurs when the activity of osteoclast surpasses that of osteoblasts, has increased in dogs. However, limited information is available on canine osteoclastogenesis. We herein described culture conditions to induce osteoclasts from canine bone marrow cells, and identified factors affecting canine osteoclastogenesis. Tartrate-resistant acid phosphatase-positive multinucleated cells were efficiently formed in a culture of bone marrow mononuclear cells with macrophage colony-stimulating factor (M-CSF 25 ng/mL) for 3 days and a subsequent culture in the presence of M-CSF (25 ng/mL) and soluble receptor activator of NF-κB ligand (RANKL 50 ng/mL) for 4 days...
April 21, 2018: Biochemical Genetics
https://www.readbyqxmd.com/read/29680264/temporary-inhibition-of-the-plasminogen-activator-inhibits-periosteal-chondrogenesis-and-promotes-periosteal-osteogenesis-during-appendicular-bone-fracture-healing
#8
D Bravo, A M Josephson, V Bradaschia-Correa, M Z Wong, N L Yim, S S Neibart, S N Lee, J Huo, T Coughlin, M M Mizrahi, P Leucht
INTRODUCTION: Aminocaproic acid is approved as an anti-fibrinolytic for use in joint replacement and spinal fusion surgeries to limit perioperative blood loss. Previous animal studies have demonstrated a pro-osteogenic effect of aminocaproic acid in spine fusion models. Here, we tested if aminocaproic acid enhances appendicular bone healing and we sought to uncover the effect of aminocaproic acid on osteoprogenitor cells (OPCs) during bone regeneration. METHODS: We employed a well-established murine femur fracture model in adult C57BL/6J mice after receiving two peri-operative injections of aminocaproic acid...
April 19, 2018: Bone
https://www.readbyqxmd.com/read/29677546/bacitracin-promotes-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-by-stimulating-the-bone-morphogenetic-protein-2-smad-axis
#9
Hui Li, Bin'en Nie, Zhe Du, Shutao Zhang, Teng Long, Bing Yue
Bacitracin, a widely used metallopeptide antibiotic, has been reported to be locally used in treating wounds without systemic adverse reactions. Our preliminary study showed that bacitracin might enhance the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). The present study investigated whether bacitracin affects the osteogenic differentiation of HBMSCs and the molecular mechanisms involved. The proliferation of HBMSCs in the presence of bacitracin was examined using a cell counting kit-8 (CCK-8) assay...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29676133/-psoralen-inhibits-raw264-7-differentiation-into-osteoclasts-and-bone-resorption-by-regulating-cd4-t-cell-differentiation
#10
Jin-Ping Li, Bao-Ping Xie, Wen-Juan Zhang, Li-Ying Shi, Wei-Juan Li, Ying Zeng, Guo-Xing Gan, Yu-Hong Li
This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676074/immune-characterization-of-bone-marrow-derived-models-of-mucosal-and-connective-tissue-mast-cells
#11
Sara Benedé, Evan Cody, Charuta Agashe, M Cecilia Berin
PURPOSE: It is well appreciated that mast cells (MCs) demonstrate tissue-specific imprinting, with different biochemical and functional properties between connective tissue MCs (CTMCs) and mucosal MCs (MMCs). Although in vitro systems have been developed to model these different subsets, there has been limited investigation into the functional characteristics of the 2 major MC subsets. Here, we report the immunologic characterization of 2 MCs subsets developed in vitro from bone marrow progenitors modeling MMCs and CTMCs...
May 2018: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/29671652/effects-of-educated-monocytes-with-xenogeneic-mesenchymal-stem-cell-derived-conditioned-medium-in-a-mouse-model-of-chronic-asthma
#12
Hadi Esmaili Gourvarchin Galeh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran, Siamak Naji Hadai
BACKGROUND: This study was conducted to determine the potential of the monocytes educated with rat bone marrow mesenchymal stem cell-derived conditioned medium (MCM) in ameliorating animal model of asthma. METHODS: Chronic asthma was induced in the BALB/c mice using ovalbumin (OVA) sensitization. The monocytes were isolated from blood of mice and supplemented with 50% MCM or negative control media. After 24 h, the cells were designated as "non-educated or educated"...
April 19, 2018: Immunological Investigations
https://www.readbyqxmd.com/read/29671649/lag-3-represents-a-marker-of-cd4-t-cells-with-regulatory-activity-in-patients-with-bone-fracture
#13
Jun Wang, Yunfan Ti, Yicun Wang, Guodong Guo, Hui Jiang, Menghan Chang, Hongbo Qian, Jianning Zhao, Guojing Sun
The lymphocyte activation gene 3 (LAG-3) is a CD4 homolog with binding affinity to MHC class II molecules. It is thought that LAG-3 exerts a bimodal function, such that co-ligation of LAG-3 and CD3 could deliver an inhibitory signal in conventional T cells, whereas, on regulatory T cells, LAG-3 expression could promote their inhibitory function. In this study, we investigated the role of LAG-3 expression on CD4+ T cells in patients with long bone fracture. We found that LAG-3+ cells represented approximately 13% of peripheral blood CD4+ T cells on average...
April 19, 2018: Immunological Investigations
https://www.readbyqxmd.com/read/29669741/shp-1-regulates-hematopoietic-stem-cell-quiescence-by-coordinating-tgf-%C3%AE-signaling
#14
Linjia Jiang, Xue Han, Jin Wang, Chen Wang, Xiaoqiang Sun, Jiayi Xie, Guojin Wu, Hiep Phan, Zhenguo Liu, ChengCheng Zhang, Meng Zhao, Xunlei Kang
Cell cycle quiescence is critical for hematopoietic stem cell (HSC) maintenance. TGF-β signaling in bone marrow niche has been identified in regulating HSC quiescence; however, the intrinsic regulatory mechanisms remain unclear. This study reports that Shp-1 knockout HSCs have attenuated quiescence and impaired long-term self-renewal. SHP-1-activated HSCs are surrounded by megakaryocytes, which regulate HSC quiescence by producing TGF-β1. Mechanistically, SHP-1 interacts with the immunoreceptor tyrosine-based inhibition motif on TGF-β receptor 1 and is critical for TGF-β signaling activation in HSCs...
April 18, 2018: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29664133/coordinated-regulation-of-the-dorsal-ventral-and-anterior-posterior-patterning-of-xenopus-embryos-by-the-btb-poz-zinc-finger-protein-zbtb14
#15
Kimiko Takebayashi-Suzuki, Hidenori Konishi, Tatsuo Miyamoto, Tomoko Nagata, Misa Uchida, Atsushi Suzuki
During early vertebrate embryogenesis, bone morphogenetic proteins (BMPs) belonging to the transforming growth factor-β (TGF-β) family of growth factors play a central role in dorsal-ventral (DV) patterning of embryos, while other growth factors such as Wnt and fibroblast growth factor (FGF) family members regulate formation of the anterior-posterior (AP) axis. Although the establishment of body plan is thought to require coordinated formation of the DV and AP axes, the mechanistic details underlying this coordination are not well understood...
April 2018: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/29662780/distinct-metabolic-programs-induced-by-tgf-%C3%AE-1-and-bmp2-in-human-articular-chondrocytes-with-osteoarthritis
#16
Cuicui Wang, Richard M Silverman, Jie Shen, Regis J O'Keefe
Objectives: Cellular energy metabolism is important for the function of all tissues, including cartilage. Recent studies indicate that superficial and deep subpopulations of articular chondrocytes (ACs) have distinct metabolic profiles. At the cellular and molecular level, osteoarthritis (OA) is characterised by alteration from a healthy homoeostatic state towards a catabolic state. Several molecular pathways, including transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP) signalling, have been identified as critical players in the pathogenesis and progression of OA...
January 2018: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29660408/expansion-of-the-clinical-spectrum-of-frontometaphyseal-dysplasia-2-caused-by-the-recurrent-mutation-p-pro485leu-in-map3k7
#17
Alice Costantini, Carina Wallgren-Pettersson, Outi Mäkitie
Frontometaphyseal dysplasia 2 (FMD2) is a skeletal dysplasia with supraorbital hyperostosis combined with undermodeling of the bones, joint contractures and some extraskeletal features. It is caused by heterozygous mutations in MAP3K7, encoding the Mitogen-Activated Protein 3-Kinase 7. MAP3K7 is activated by TGF-β and plays an important role in osteogenesis. Less than 20 patients with FMD2 and MAP3K7 mutations have been described thus far. The majority of the patients harbor a recurrent missense mutation, NM_003188...
April 13, 2018: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29656307/alveolar-bone-protective-effect-of-hiziki-extracts-on-the-progression-of-periodontitis
#18
Don-Gil Lee, Yu-Kyong Shin, Jae-Hee Park, Sang-Yong Park, Eunson Hwang, Jung-Eun Yang, Hae Jo, Ki-Young Kim, Gafurjon T Mavlonov, Tae-Hoo Yi
The purpose of this study was to evaluate the effects of hiziki extract on alveolar bone loss, inflammation, and osteo-biomarker expression in hPDL cells (10, 50, 100 μg/ml final concentrations in culture medium) and on a ligature-induced periodontitis rat model (50, 100, 200 mg/kg with oral administration). Hiziki extract increased alkaline phosphatase activity and mineralized nodule formation in hPDL cell. In western blot analysis, hiziki extract resulted in increased expression of osteoblast markers, including transforming growth factor beta (TGF-β), SMAD anchor for receptor activation (SARA) and runt-related transcription factor 2 (RUNX2) in hDPL cells...
April 14, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29652075/impact-of-tgf-%C3%AE-family-related-growth-factors-on-chondrogenic-differentiation-of-adipose-derived-stem-cells-isolated-from-lipoaspirates-and-infrapatellar-fat-pads-of-osteoarthritic-patients
#19
E López-Ruiz, G Jiménez, W Kwiatkowski, E Montañez, F Arrebola, E Carrillo, S Choe, J A Marchal, M Perán
The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondrogenic capacity of mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) and the infrapatellar fat pad (IFPSCs) of osteoarthritic patients and treated with transforming growth factor (TGF)-β family-related growth factors...
April 13, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29644573/myeloid-specific-targeting-of-notch-ameliorates-murine-renal-fibrosis-via-reduced-infiltration-and-activation-of-bone-marrow-derived-macrophage
#20
Yali Jiang, Yuanyuan Wang, Pengfei Ma, Dongjie An, Junlong Zhao, Shiqian Liang, Yuchen Ye, Yingying Lu, Peng Zhang, Xiaowei Liu, Hua Han, Hongyan Qin
Macrophages play critical roles in renal fibrosis. However, macrophages exhibit ontogenic and functional heterogeneities, and which population of macrophages contributes to renal fibrosis and the underlying mechanisms remain unclear. In this study, we genetically targeted Notch signaling by disrupting the transcription factor recombination signal binding protein-Jκ (RBP-J), to reveal its role in regulation of macrophages during the unilateral ureteral obstruction (UUO)-induced murine renal fibrosis. Myeloid-specific disruption of RBP-J attenuated renal fibrosis with reduced extracellular matrix deposition and myofibroblast activation, as well as attenuated epithelial-mesenchymal transition, likely owing to the reduced expression of TGF-β...
April 11, 2018: Protein & Cell
keyword
keyword
60734
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"