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purified umbilical stem cells

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https://www.readbyqxmd.com/read/27515026/potential-role-of-herbal-remedies-in-stem-cell-therapy-proliferation-and-differentiation-of-human-mesenchymal-stromal-cells
#1
REVIEW
Vindya Lankika Udalamaththa, Chanika Dilumi Jayasinghe, Preethi Vidya Udagama
Stem cell therapy has revolutionized modern clinical therapy with the potential of stem cells to differentiate into many different cell types which may help to replace different cell lines of an organism. Innumerous trials are carried out to merge new scientific knowledge and techniques with traditional herbal extracts that may result in less toxic, affordable, and highly available natural alternative therapeutics. Currently, mesenchyamal stromal cell (MSC) lines are treated with individual and mixtures of crude herbal extracts, as well as with purified compounds from herbal extracts, to investigate the mechanisms and effects of these on stem cell growth and differentiation...
August 11, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27424784/ectopic-mir-125a-expression-induces-long-term-repopulating-stem-cell-capacity-in-mouse-and-human-hematopoietic-progenitors
#2
Edyta E Wojtowicz, Eric R Lechman, Karin G Hermans, Erwin M Schoof, Erno Wienholds, Ruth Isserlin, Peter A van Veelen, Mathilde J C Broekhuis, George M C Janssen, Aaron Trotman-Grant, Stephanie M Dobson, Gabriela Krivdova, Jantje Elzinga, James Kennedy, Olga I Gan, Ankit Sinha, Vladimir Ignatchenko, Thomas Kislinger, Bertien Dethmers-Ausema, Ellen Weersing, Mir Farshid Alemdehy, Hans W J de Looper, Gary D Bader, Martha Ritsema, Stefan J Erkeland, Leonid V Bystrykh, John E Dick, Gerald de Haan
Umbilical cord blood (CB) is a convenient and broadly used source of hematopoietic stem cells (HSCs) for allogeneic stem cell transplantation. However, limiting numbers of HSCs remain a major constraint for its clinical application. Although one feasible option would be to expand HSCs to improve therapeutic outcome, available protocols and the molecular mechanisms governing the self-renewal of HSCs are unclear. Here, we show that ectopic expression of a single microRNA (miRNA), miR-125a, in purified murine and human multipotent progenitors (MPPs) resulted in increased self-renewal and robust long-term multi-lineage repopulation in transplanted recipient mice...
September 1, 2016: Cell Stem Cell
https://www.readbyqxmd.com/read/27244897/mesenchymal-stem-cell-like-properties-of-cd133-glioblastoma-initiating-cells
#3
Lorena Favaro Pavon, Tatiana Tais Sibov, Daniela Mara de Oliveira, Luciana C Marti, Francisco Romero Cabral, Jean Gabriel de Souza, Pamela Boufleur, Suzana M F Malheiros, Manuel A de Paiva Neto, Edgard Ferreira da Cruz, Ana Marisa Chudzinski-Tavassi, Sérgio Cavalheiro
Glioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal properties of primary highly purified human CD133+ glioblastoma-initiating cells. To accomplish this aim, we used the following approaches: i) generation of tumor subspheres of CD133+ selected cells from primary cell cultures of glioblastoma; ii) analysis of the expression of pluripotency stem cell markers and mesenchymal stem cell (MSC) markers in the CD133+ glioblastoma-initiating cells; iii) side-by-side ultrastructural characterization of the CD133+ glioblastoma cells, MSC and CD133+ hematopoietic stem cells isolated from human umbilical cord blood (UCB); iv) assessment of adipogenic differentiation of CD133+ glioblastoma cells to test their MSC-like in vitro differentiation ability; and v) use of an orthotopic glioblastoma xenograft model in the absence of immune suppression...
June 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27185256/dna-hypermethylation-of-cd3-t-cells-from-cord-blood-of-infants-exposed-to-intrauterine-growth-restriction
#4
Lyda Williams, Yoshinori Seki, Fabien Delahaye, Alex Cheng, Mamta Fuloria, Francine Hughes Einstein, Maureen J Charron
AIMS/HYPOTHESIS: Intrauterine growth restriction (IUGR) is associated with increased susceptibility to obesity, metabolic syndrome and type 2 diabetes. Although the mechanisms underlying the developmental origins of metabolic disease are poorly understood, evidence suggests that epigenomic alterations play a critical role. We sought to identify changes in DNA methylation patterns that are associated with IUGR in CD3(+) T cells purified from umbilical cord blood obtained from male newborns who were appropriate for gestational age (AGA) or who had been exposed to IUGR...
August 2016: Diabetologia
https://www.readbyqxmd.com/read/27146030/endometrial-mesenchymal-stem-stromal-cells-their-fibroblast-progeny-in-endometriosis-and-more
#5
Caroline E Gargett, Shanti Gurung
No abstract text is available yet for this article.
June 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/26679930/isolation-characterisation-and-comparative-analysis-of-human-umbilical-cord-vein-perivascular-cells-and-cord-blood-mesenchymal-stem-cells
#6
Beyza Gökçinar-Yagci, Özgür Özyüncü, Betül Çelebi-Saltik
Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In this study, our aim is to isolate perivascular cells from human UC vein and characterize them versus UC blood MSCs (UCB-MSCs). For this purpose, perivascular cells around the UC vein were isolated enzymatically and then purified with magnetic activated cell sorting (MACS) method using CD146 Microbead Kit respectively...
June 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/26661850/expression-analysis-of-radiation-responsive-genes-in-human-hematopoietic-stem-progenitor-cells
#7
Takakiyo Tsujiguchi, Tokuhisa Hirouchi, Satoru Monzen, Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo, Ikuo Kashiwakura
To clarify the nature of the genes that contribute to the radiosensitivity of human hematopoietic stem/progenitor cells (HSPCs), we analyzed the gene expression profiles detected in HSPCs irradiated with 2 Gy X-rays after culture with or without an optimal combination of hematopoietic cytokines. Highly purified CD34(+) cells from human placental/umbilical cord blood were used as HSPCs. The cells were exposed to 2 Gy X-irradiation and treated in serum-free medium under five different sets of conditions for 6 h...
January 2016: Journal of Radiation Research
https://www.readbyqxmd.com/read/26635882/improvement-in-isolation-and-identification-of-mouse-oogonial-stem-cells
#8
Zhiyong Lu, Meng Wu, Jinjin Zhang, Jiaqiang Xiong, Jing Cheng, Wei Shen, Aiyue Luo, Li Fang, Shixuan Wang
Female germline stem cells (FGSCs) or oogonial stem cells (OSCs) have the capacity to generate newborn oocytes and thus open a new door to fight ovarian aging and female infertility. However, the production and identification of OSCs are difficult for investigators. Rare amount of these cells in the ovary results in the failure of the acquisition of OSCs. Furthermore, the oocyte formation by OSCs in vivo was usually confirmed using tissue sections by immunofluorescence or immunohistochemistry in previous studies...
2016: Stem Cells International
https://www.readbyqxmd.com/read/26199900/in-vitro-differentiation-of-human-umbilical-cord-blood-cd133-cells-into-insulin-producing-cells-in-co-culture-with-rat-pancreatic-mesenchymal-stem-cells
#9
Fazel Sahraneshin Samani, Marzieh Ebrahimi, Tahereh Zandieh, Reyhaneh Khoshchehreh, Mohamadreza Baghaban Eslaminejad, Nasser Aghdami, Hossein Baharvand
OBJECTIVE: Pancreatic stroma plays an important role in the induction of pancreatic cells by the use of close range signaling. In this respect, we presume that pancreatic mesenchymal cells (PMCs) as a fundamental factor of the stromal niche may have an effective role in differentiation of umbilical cord blood cluster of differentiation 133(+) (UCB-CD133(+)) cells into newly-formed β-cells in vitro. MATERIALS AND METHODS: This study is an experimental research. The UCB-CD133(+)cells were purified by magnetic activated cell sorting (MACS) and differentiated into insulin producing cells (IPCs) in co-culture, both directly and indirectly with rat PMCs...
2015: Cell Journal
https://www.readbyqxmd.com/read/26134010/-vascular-endothelial-injury-induced-by-anti-endothelial-cell-antibody-in-allogeneic-hematopoietic-stem-cell-transplantation
#10
Jianfeng Yao, Axia Song, Zheng Ruan, Lukun Zhou, Peng Liu, Haiyan Zhu, Haiyan Gong, Shuxu Dong, Yuanfu Xu, Erlie Jiang, Aiming Pang, Sizhou Feng, Mingzhe Han
OBJECTIVE: To clarify the role of endothelial cells (ECs) injury induced by anti-endothelial cell antibody (AECA) in allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: Serum immunoglobulin (IgG) from allo-HSCT recipients were purified and incubated with human umbilical vein vascular endothelium (HUVEC) in vitro, then the functional changes and cell apoptosis were tested. RESULTS: After incubation with AECA positive IgG, soluble adhesion molecules significantly elevated in culture supernatant...
June 2015: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/26044732/inhibitor-of-p53-p21-pathway-induces-the-differentiation-of-human-umbilical-cord-derived-mesenchymal-stem-cells-into-cardiomyogenic-cells
#11
Zhong-Bao Ruan, Li Zhu, Yi-Gang Yin, Ge-Cai Chen
P53 is shown recently to play an important role in the proliferation and differentiation of mesenchymal stem cells. In this study, human umbilical cord derived mesenchymal stem cells (hUCMSCs) were isolated and purified from the umbilical cords of normal or cesarean term deliveries, after treatment with 20 μmol/L PFT-α for 24 h, hUCMSCs were continued to be cultured for 4 weeks, cardiac-specific protein expression of cTnI, Desmin and Nkx2.5 was determined using immunofluorescence assay and RT-PCR. The expression of p53 and p21 was detected by western blot...
August 2016: Cytotechnology
https://www.readbyqxmd.com/read/25776756/evaluation-of-the-efficiency-of-human-immune-system-reconstitution-in-nsg-mice-and-nsg-mice-containing-a-human-hla-a2-transgene-using-hematopoietic-stem-cells-purified-from-different-sources
#12
John Patton, Raja Vuyyuru, Amanda Siglin, Michael Root, Tim Manser
Severely immunodeficient mice such as the NOD/SCID/IL2rγ(null) (NSG) strain can be engrafted with human hematopoietic stem cells (HSCs), resulting in chimeric mice containing many components of the human immune system (Human Immune System mice or HIS mice). HIS mice can both support the replication of and recapitulate much of the immunological response to a variety of pathogens, including ones with strict human tropism, such as HIV-1. In an effort to develop a better mouse model for human infectious pathogen infection and possible immune resolution, we compared the human immune system reconstitution of NSG mice following injection with human CD34(+) HSCs purified from either fetal liver (FL) or umbilical cord blood (UCB)...
July 2015: Journal of Immunological Methods
https://www.readbyqxmd.com/read/25649907/double-labelling-of-human-umbilical-cord-mesenchymal-stem-cells-with-gd-dtpa-and-pkh26-and-the-influence-on-biological-characteristics-of-hucmscs
#13
Hanlin Shuai, Changzheng Shi, Jifa Lan, Danliang Chen, Xin Luo
The aim of this study was to determine whether double labelling of human umbilical cord mesenchymal stem cells (hUCMSCs) with gadolinium-diethylene triamine penta-acetic acid (Gd-DTPA) and PKH26 influences their biological characteristics. A tissue adherence technique was used to separate and purify the hUCMSCs and flow cytometry was performed to detect the surface markers expressed on them. Gd-DTPA and PKH26 were used to label the stem cells and MRI and fluorescence microscopy were used to detect the double-labelled hUCMSCs...
February 2015: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/25369806/differentiation-of-schwann%C3%A2-like-cells-from-human-umbilical-cord-blood-mesenchymal-stem-cells-in-vitro
#14
Yu-Zhou Xiao, Sheng Wang
The use of artificial nerves for the repair of peripheral nerve defects is restricted by the limited sources of Schwann cells (SCs). Human mesenchymal stem cell (MSC)‑derived Schwann‑like cells are considered an alternative and desirable cell source. The aim of the present study was to establish a method of inducing directional differentiation of human umbilical cord blood (hUCB) MSCs into Schwann‑like cells. Cells isolated from hUCB were cultured in MesenCult complete medium, a specialized culture medium for MSCs, to expand hUCBMSCs...
February 2015: Molecular Medicine Reports
https://www.readbyqxmd.com/read/25343938/expression-and-significance-of-dll4-notch-signaling-pathway-in-the-differentiation-of-human-umbilical-cord-derived-mesenchymal-stem-cells-into-cardiomyocytes-induced-by-5-azacytidine
#15
Li Zhu, Zhongbao Ruan, Yigang Yin, Gecai Chen
hUCMSCs were isolated and purified from the umbilical cords of normal or cesarean term deliveries under sterile conditions. Flow cytometry analysis revealed that CD13, CD29, CD44, CD90, and CD105 were highly expressed on the surface of passage-3 hUCMSCs, but negative for CD31, CD34, CD45, and HLA-DR. Immunocytochemistry showed that 5-azacytidine (5-aza) could induce the cTnI expression of hUCMSCs. RT-PCR showed that a stable higher level expression of DLL4 and Notch1 gene in 5-aza-induced group was observed compared to that in the control group...
January 2015: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/25339323/-effect-of-endothelial-cell-targeted-soluble-notch-ligand-hd1r-protein-on-expansion-and-engraftment-of-cord-blood-hematopoietic-stem-progenitor-cells
#16
Dengmei Tian, Yingmin Liang, Hua Han, Yongqing Zhang
OBJECTIVE: To evaluate the effects of endothelial cell- targeted soluble Notch ligand hD1R protein on expansion and engraftment of cord blood hematopoietic stem/progenitor cell (CB HSPCs). METHODS: Recombinant hD1R protein was first induced and purified. Human cord blood CD34⁺ cells were co-cultured on human umbilical vein endothelial cells (HUVECs) supplemented with a cocktail containing 5 types of human cytokines including TPO, SCF, FL, IL-6, IL-3 (5GF) and soluble hD1R...
October 2014: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/25270685/cell-differentiation-mediated-by-co-culture-of-human-umbilical-cord-blood-stem-cells-with-murine-hepatic-cells
#17
Maria Stecklum, Annika Wulf-Goldenberg, Bettina Purfürst, Antje Siegert, Marlen Keil, Klaus Eckert, Iduna Fichtner
In the present study, purified human cord blood stem cells were co-cultivated with murine hepatic alpha mouse liver 12 (AML12) cells to compare the effect on endodermal stem cell differentiation by either direct cell-cell interaction or by soluble factors in conditioned hepatic cell medium. With that approach, we want to mimic in vitro the situation of preclinical transplantation experiments using human cells in mice. Cord blood stem cells, cultivated with hepatic conditioned medium, showed a low endodermal differentiation but an increased connexin 32 (Cx32) and Cx43, and cytokeratin 8 (CK8) and CK19 expression was monitored by reverse transcription polymerase chain reaction (RT-PCR)...
February 2015: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/25256682/s100a6-is-secreted-from-wharton-s-jelly-mesenchymal-stem-cells-and-interacts-with-integrin-%C3%AE-1
#18
Ewelina Jurewicz, Agnieszka Góral, Anna Filipek
S100A6 is a calcium binding protein belonging to the S100 family. In this work we examined the function of extracellular S100A6. Using mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord (WJMS cells) we have shown that S100A6 is secreted by these cells, and when added to the medium, increases their adhesion and inhibits proliferation. The search for a potential target/receptor of S100A6 in the membrane fraction of WJMS cells allowed us to identify some proteins, among them integrin β1, which interacts with S100A6 in a calcium dependent manner...
October 2014: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/25232508/hematopoietic-stem-cells-derived-from-human-umbilical-cord-ameliorate-cisplatin-induced-acute-renal-failure-in-rats
#19
Rokaya H Shalaby, Laila A Rashed, Alaa E Ismaail, Naglaa K Madkour, Sherien H Elwakeel
Injury to a target organ can be sensed by bone marrow stem cells that migrate to the site of damage, undergo differentiation, and promote structural and functional repair. This remarkable stem cell capacity prompted an investigation of the potential of mesenchymal and hematopoietic stem cells to cure acute renal failure. On the basis of the recent demonstration that hematopoietic stem cells (HSCs) can differentiate into renal cells, the current study tested the hypothesis that HSCs can contribute to the regeneration of renal tubular epithelial cells after renal injury...
2014: American Journal of Stem Cells
https://www.readbyqxmd.com/read/25232467/the-assessment-of-cd146-based-cell-sorting-and-telomere-length-analysis-for-establishing-the-identity-of-mesenchymal-stem-cells-in-human-umbilical-cord
#20
Dimitrios Kouroupis, Sarah M Churchman, Dennis McGonagle, Elena A Jones
Adult stem cells are characterised by longer telomeres compared to mature cells from the same tissue. In this study, candidate CD146 (+) umbilical cord (UC) mesenchymal stem cells (MSCs) were purified by cell sorting from UC tissue digests and their telomere lengths were measured in comparison to donor-matched CD146-negative fraction. UC tissue fragments were enzymatically treated with collagenase and the cells were used for cell sorting, colony-forming fibroblast (CFU-F) assay or for long-term MSC cultivation...
2014: F1000Research
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