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https://www.readbyqxmd.com/read/28538846/-clinical-and-microbiological-study-of-catheter-associated-urinary-tract-infections-in-internal-medicine-services-of-a-venezuelan-university-hospital
#1
Pedro Quijada-Martínez, Ana Flores-Carrero, Indira Labrador, María Araque
Objectives: To determine the clinical and microbiological characteristics of catheter-associated urinary tract infections (CA-UTI) in patients admitted to the Internal Medicine services of the Hospital Universitario de Los Andes (HULA), Mérida, Venezuela and to establish the clonal distribution of multi-resistant Enterobacteriaceae that produce this infection. Materials and Methods: Seventy-three adult patients with bladder catheterization were studied between January and July 2015...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28535205/a-transcriptional-serenaid-the-role-of-noncoding-rnas-in-class-switch-recombination
#2
William T Yewdell, Jayanta Chaudhuri
During an immune response, activated B cells may undergo class switch recombination (CSR), a molecular rearrangement that allows B cells to switch from expressing IgM and IgD to a secondary antibody heavy chain isotype such as IgG, IgA, or IgE. Secondary antibody isotypes provide the adaptive immune system with distinct effector functions to optimally combat various pathogens. CSR occurs between repetitive DNA elements within the immunoglobulin heavy chain (Igh) locus, termed switch (S) regions, and requires the DNA modifying enzyme activation-induced cytidine deaminase (AID)...
May 23, 2017: International Immunology
https://www.readbyqxmd.com/read/28531368/molecular-phylogeny-of-loliginidae-inferred-from-mitochondrial-dna-sequence-variation
#3
Lisen Kang, Shuyi Zhang, Changwen Wu, Xiaoxu Liu, Mei Ying Xu, Lihua Jiang
Loliginidae includes many economically important species in trophic systems worldwide. Here, we investigated genetic relationships and diversity in this family. Sequence comparisons and phylogenetic analyses revealed considerable variations between mitochondrial 16 S rRNA gene and cytochrome coxidase subunit I gene among nine Loliginid species. We identified three similar non-coding regions in eight Loliginid species, but not in Sepioteuthislessoniana. We detected a single extended termination-associated sequence and three conserved sequence blocks among these eight species...
May 20, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28529469/a-comparative-genomics-approach-revealed-evolutionary-dynamics-of-microsatellite-imperfection-and-conservation-in-genus-gossypium
#4
Muhammad Mahmood Ahmed, Chao Shen, Anam Qadir Khan, Muhammad Atif Wahid, Muhammad Shaban, Zhongxu Lin
BACKGROUND: Ongoing molecular processes in a cell could target microsatellites, a kind of repetitive DNA, owing to length variations and motif imperfection. Mutational mechanisms underlying such kind of genetic variations have been extensively investigated in diverse organisms. However, obscure impact of ploidization, an evolutionary process of genome content duplication prevails mostly in plants, on non-coding DNA is poorly understood. RESULTS: Genome sequences of diversely originated plant species were examined for genome-wide motif imperfection pattern, and various analytical tools were employed to canvass characteristic relationships among repeat density, imperfection and length of microsatellites...
2017: Hereditas
https://www.readbyqxmd.com/read/28527285/-genetic-diversity-of-extraintestinal-escherichia-coli-strains-producers-of-beta-lactamases-tem-shv-and-ctx-m-associated-with-healthcare
#5
Yasmin Varela, Beatriz Millán, María Araque
INTRODUCTION: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. OBJECTIVE: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. MATERIALS AND METHODS: We studied a collection of 12 strains of extraintestinal E...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28515439/the-biomechanics-behind-extreme-osteophagy-in-tyrannosaurus-rex
#6
Paul M Gignac, Gregory M Erickson
Most carnivorous mammals can pulverize skeletal elements by generating tooth pressures between occluding teeth that exceed cortical bone shear strength, thereby permitting access to marrow and phosphatic salts. Conversely, carnivorous reptiles have non-occluding dentitions that engender negligible bone damage during feeding. As a result, most reptilian predators can only consume bones in their entirety. Nevertheless, North American tyrannosaurids, including the giant (13 metres [m]) theropod dinosaur Tyrannosaurus rex stand out for habitually biting deeply into bones, pulverizing and digesting them...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28514774/demonstration-of-5-methylcytosine-rich-dna-sequences-in-chiroptera
#7
Michael Schmid, Claus Steinlein, Christian Lomb, Marianne Volleth
5-Methylcytosine-rich heterochromatic regions were demonstrated in metaphase chromosomes of 5 species of Chiroptera by indirect immunofluorescence using a monoclonal anti-5-methylcytosine antibody. These species belong to 4 genera and 2 families and are characterized by divergent karyotypes. One species (Glauconycteris beatrix) has an extremely low diploid chromosome number of 2n = 22 with only meta- to submetacentric elements and remarkably large amounts of constitutive heterochromatin located in the centromeric and pericentromeric regions of all chromosome pairs...
May 18, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28506673/high-frequency-of-silver-resistance-genes-in-invasive-isolates-of-enterobacter-and-klebsiella-species
#8
S Sütterlin, M Dahlö, C Tellgren-Roth, W Schaal, Å Melhus
BACKGROUND: Silver-based products have been marketed as an alternative to antibiotics, and their consumption has increased. Bacteria may, however, develop resistance to silver. AIM: To study the presence of genes encoding silver resistance (silE, silP, silS) over time in three clinically important Enterobacteriaceae genera. METHODS: Using polymerase chain reaction (PCR), 752 bloodstream isolates from the years 1990-2010 were investigated. Age, gender, and ward of patients were registered, and the susceptibility to antibiotics and silver nitrate was tested...
April 26, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28505302/the-first-whole-genome-and-transcriptome-of-the-manila-clam-ruditapes-philippinarum-isolated-in-korea
#9
Seyoung Mun, Yun-Ji Kim, Kesavan Markkandan, Wonseok Shin, Sumin Oh, Jiyoung Woo, Jongsu Yoo, Hyesuck An, Kyudong Han
The manila clam, Ruditapes philippinarum, is an important bivalve species in worldwide aquaculture including Korea. The aquaculture production of R. philippinarum is under threat from diverse environmental factors including viruses, microorganisms, parasites, and water conditions with subsequently declining production. In spite of its importance as a marine resource, the reference genome of R. philippinarum for comprehensive genetic studies is largely unexplored. Here, we report the de novo whole-genome and transcriptome assembly of R...
May 13, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28501200/a-new-approach-to-calibration-and-determination-of-selected-trace-elements-in-food-contact-polymers-by-la-icp-ms
#10
Monica Voss, Matheus A G Nunes, Gabriela Corazza, Erico M M Flores, Edson I Müller, Valderi L Dressler
A calibration strategy using porous nylon disks and reference solutions is proposed for the first time for matrix matching and determination of As, Ba, Cd, Cr, Pb, Sr and Zn in polymers by laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS). Polymer samples commonly used in contact with food were analyzed. The procedure consists on the use of porous nylon disks as support for a dried droplet reference solution. Calibration in the range of 0.50-190μgg(-1) for Ba, Cd, Cr, Pb, Sr and Zn and from 0...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28500844/cell-free-circulating-dna-integrity-is-an-independent-predictor-of-impending-breast-cancer-recurrence
#11
Jie Cheng, Katarina Cuk, Jörg Heil, Michael Golatta, Sarah Schott, Christof Sohn, Andreas Schneeweiss, Barbara Burwinkel, Harald Surowy
Non-invasive blood-based molecule markers are evaluated as promising biomarkers these days. Here we investigated the potential of cell-free circulating DNA Integrity (cfDI) as blood-based marker for the prediction of recurrence during the follow-up of breast cancer patients within a prospective study cohort. cfDI was determined in plasma of 212 individuals, by measuring ALU and LINE1 repetitive DNA elements using quantitative PCR. A significant decrease of cfDI in recurrent breast cancer patients was observed...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28497530/short-term-memory-in-childhood-dyslexia-deficient-serial-order-in-multiple-modalities
#12
Nelson Cowan, Tiffany P Hogan, Mary Alt, Samuel Green, Kathryn L Cabbage, Shara Brinkley, Shelley Gray
In children with dyslexia, deficits in working memory have not been well-specified. We assessed second-grade children with dyslexia, with and without concomitant specific language impairment, and children with typical development. Immediate serial recall of lists of phonological (non-word), lexical (digit), spatial (location) and visual (shape) items were included. For the latter three modalities, we used not only standard span but also running span tasks, in which the list length was unpredictable to limit mnemonic strategies...
May 12, 2017: Dyslexia: the Journal of the British Dyslexia Association
https://www.readbyqxmd.com/read/28491069/characterization-of-the-polycomb-group-mark-h3k27me3-in-unicellular-algae
#13
Pawel Mikulski, Olga Komarynets, Fabio Fachinelli, Andreas P M Weber, Daniel Schubert
Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and target genes in unicellular branches of the green lineage remain largely unknown. To shed light on PcG function and modus operandi in a broad evolutionary context, we demonstrate phylogenetic relationship of core PRC1 and PRC2 proteins and H3K27me3 biochemical presence in several unicellular algae of different phylogenetic subclades...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28486975/evidence-for-transcriptome-wide-rna-editing-among-sus-scrofa-pre-1-sine-elements
#14
Scott A Funkhouser, Juan P Steibel, Ronald O Bates, Nancy E Raney, Darius Schenk, Catherine W Ernst
BACKGROUND: RNA editing by ADAR (adenosine deaminase acting on RNA) proteins is a form of transcriptional regulation that is widespread among humans and other primates. Based on high-throughput scans used to identify putative RNA editing sites, ADAR appears to catalyze a substantial number of adenosine to inosine transitions within repetitive regions of the primate transcriptome, thereby dramatically enhancing genetic variation beyond what is encoded in the genome. RESULTS: Here, we demonstrate the editing potential of the pig transcriptome by utilizing DNA and RNA sequence data from the same pig...
May 9, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28486658/a-high-coverage-draft-genome-of-the-mycalesine-butterfly-bicyclus-anynana
#15
Reuben W Nowell, Ben Elsworth, Vicencio Oostra, Bas J Zwaan, Christopher W Wheat, Marjo Saastamoinen, Ilik J Saccheri, Arjen E Van't Hof, Bethany R Wasik, Heidi Connahs, Muhammad L Aslam, Sujai Kumar, Richard J Challis, Antónia Monteiro, Paul M Brakefield, Mark Blaxter
Background: The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Findings: Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology...
May 9, 2017: GigaScience
https://www.readbyqxmd.com/read/28485713/super-resolution-imaging-of-a-2-5-kb-non-repetitive-dna-in-situ-in-the-nuclear-genome-using-molecular-beacon-probes
#16
Yanxiang Ni, Bo Cao, Tszshan Ma, Gang Niu, Yingdong Huo, Jiandong Huang, Danni Chen, Yi Liu, Bin Yu, Michael Q Zhang, Hanben Niu
High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaint probes have enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence...
May 9, 2017: ELife
https://www.readbyqxmd.com/read/28484201/epigenetic-analysis-of-bovine-parthenogenetic-embryonic-fibroblasts
#17
Masahiro Kaneda, Masashi Takahashi, Ken-Ichi Yamanaka, Koji Saito, Masanori Taniguchi, Satoshi Akagi, Shinya Watanabe, Takashi Nagai
Although more than 100 imprinted genes have already been identified in the mouse and human genomes, little is known about genomic imprinting in cattle. For a better understanding of these genes in cattle, parthenogenetically activated bovine blastocysts were transferred to recipient cows to obtain parthenotes, and fibroblasts derived from a Day 40 (Day 0 being the day of parthenogenetic activation) parthenogenetic embryo (BpEFs) were successfully obtained. Bovine embryonic fibroblasts (BEFs) were also isolated from a normal fertilized embryo obtained from an artificially inseminated cow...
May 6, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28475810/draft-genome-sequence-of-the-tibetan-medicinal-herb-rhodiola-crenulata
#18
Yuanyuan Fu, Liangwei Li, Shijie Hao, Rui Guan, Guangyi Fan, Chengcheng Shi, Haibo Wan, Wenbin Chen, He Zhang, Guocheng Liu, Jihua Wang, Lulin Ma, Jianling You, Xuemei Ni, Zhen Yue, Xun Xu, Xiao Sun, Xin Liu, Simon Ming-Yuen Lee
Background: Rhodiola crenulata , a well-known medicinal Tibetan herb, is mainly grown in high-altitude regions of Tibet, Yunnan and Sichuan provinces in China. In the past few years increasing numbers of studies have been published on the potential pharmacological activities of R. crenulata , strengthening our understanding into its putitive active ingredient composition, pharmacological activity and mechanism of action. These findings also provide strong evidence supporting the important medicinal and economical value of R...
May 5, 2017: GigaScience
https://www.readbyqxmd.com/read/28472109/enherv-enrichment-analysis-of-specific-human-endogenous-retrovirus-patterns-and-their-neighboring-genes
#19
Pumipat Tongyoo, Yingyos Avihingsanon, Santhitham Prom-On, Apiwat Mutirangura, Wuttichai Mhuantong, Nattiya Hirankarn
Human endogenous retroviruses (HERVs) are flanked by long terminal repeats (LTRs), which contain the regulation part of the retrovirus. Remaining HERVs constitute 7% to 8% of the present day human genome, and most have been identified as solo LTRs. The HERV sequences have been associated with several molecular functions as well as certain diseases in human, but their roles in human diseases are yet to be established. We designed EnHERV to make accessible the identified endogenous retrovirus repetitive sequences from Repbase Update (a database of eukaryotic repetitive elements) that are present in the human genome...
2017: PloS One
https://www.readbyqxmd.com/read/28471386/transposable-elements-in-human-cancer-causes-and-consequences-of-deregulation
#20
REVIEW
Sumadi Lukman Anwar, Wahyu Wulaningsih, Ulrich Lehmann
Transposable elements (TEs) comprise nearly half of the human genome and play an essential role in the maintenance of genomic stability, chromosomal architecture, and transcriptional regulation. TEs are repetitive sequences consisting of RNA transposons, DNA transposons, and endogenous retroviruses that can invade the human genome with a substantial contribution in human evolution and genomic diversity. TEs are therefore firmly regulated from early embryonic development and during the entire course of human life by epigenetic mechanisms, in particular DNA methylation and histone modifications...
May 4, 2017: International Journal of Molecular Sciences
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