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miRNA, microRNA, Colorectal cancer

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https://www.readbyqxmd.com/read/27906435/down-regulation-of-microrna-143-is-associated-with-colorectal-cancer-progression
#1
J-W Bai, H-Z Xue, C Zhang
OBJECTIVE: Colorectal cancer (CRC) is one of the most prevalent carcinomas worldwide. Tumor metastasis and recurrence are leading causes of CRC-related deaths. Given the role of microRNA (miRNA) in CRC invasion and metastasis, we explored the association between miRNA-143 expression and clinicopathologic characteristics in CRC, as well as the effects of miRNA-143 on CRC cell invasion in vitro. MATERIALS AND METHODS: Quantitative real-time PCR was conducted to assess the expression of miR-143 in tissue specimens and cell lines...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27904764/mir-193b-directly-targets-stmn1-and-inhibits-the-malignant-phenotype-in-colorectal-cancer
#2
Feng Guo, Yang Luo, Yi-Fei Mu, Shao-Lan Qin, Yang Qi, Yi-Er Qiu, Ming Zhong
Accumulating evidence suggests that aberrantly expressed microRNAs (miRNAs) contribute to the initiation and progression of human cancers. However, the underlying function of miR-193b in colorectal cancer (CRC) remains largely unexplored. Herein, we demonstrate that miR-193b is significantly down-regulated in CRC tissues compared with their normal counterparts. Kaplan-Meier analysis revealed that decreased miR-193b expression was closely associated with the shorter overall survival of patients with CRC. Through gain-and loss-of-function studies, we showed that miR-193b significantly suppressed CRC cell proliferation and invasion...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27903980/identification-of-biomarker-micrornas-for-predicting-the-response-of-colorectal-cancer-to-neoadjuvant-chemoradiotherapy-based-on-microrna-regulatory-network
#3
Yaqun Zhu, Qiliang Peng, Yuxin Lin, Li Zou, Peipei Shen, Feifei Chen, Ming Min, Li Shen, Jiajia Chen, Bairong Shen
Preoperative radiotherapy or chemoradiotherapy has become a standard procedure for treatment of patients with locally advanced colorectal cancer (CRC). However, patients' responses to treatment are different and personalized. MicroRNAs (miRNAs) are promising biomarkers for predicting personalized responses. In this study, we collected 30 publicly reported miRNAs associated with chemoradiotherapy of CRC. We extracted 46 differentially expressed miRNAs from samples of responders and non-responders to preoperative radiotherapy from the Gene Expression Omnibus dataset (Student's t test, p-value < 0...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27902469/the-mir-196b-mirna-inhibits-the-gata6-intestinal-transcription-factor-and-is-upregulated-in-colon-cancer-patients
#4
Sebastian Fantini, Valentina Salsi, Luca Reggiani, Antonino Maiorana, Vincenzo Zappavigna
OBJECTIVE: To explore the possible misexpression of the microRNA miR-196b in colorectal cancer (CRC) and its role in controlling the expression of GATA6, a putative target gene crucial to intestinal cell homeostasis and tumorigenesis. DESIGN: The expression of miR-196b was analysed by qRT-PCR in surgical resection samples from a cohort of sporadic colon cancer patients. Manipulations of miR-196b expression were performed to demonstrate its inhibition of GATA6 protein levels...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27896992/microrna-augmented-pathways-mirap-and-their-applications-to-pathway-analysis-and-disease-subtyping
#5
Diana Diaz, Michele Donato, Tin Nguyen, Sorin Draghici
MicroRNAs play important roles in the development of many complex diseases. Because of their importance, the analysis of signaling pathways including miRNA interactions holds the potential for unveiling the mechanisms underlying such diseases. However, current signaling pathway databases are limited to interactions between genes and ignore miRNAs. Here, we use the information on miRNA targets to build a database of miRNA-augmented pathways (mirAP), and we show its application in the contexts of integrative pathway analysis and disease subtyping...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27894087/negative-feedback-between-tap63-and-mir-133b-mediates-colorectal-cancer-suppression
#6
Jing Dai, Hao Wu, Yi Zhang, Kai Gao, Gui Hu, Yihang Guo, Changwei Lin, Xiaorong Li
BACKGROUND: TAp63 is known as the most potent transcription activator and tumor suppressor. microRNAs (miRNAs) are increasingly recognized as essential components of the p63 pathway, mediating downstream post-transcriptional gene repression. The aim of present study was to investigate a negative feedback loop between TAp63 and miR-133b. RESULTS: Overexpression of TAp63 inhibited HCT-116 cell proliferation, apoptosis and invasion via miR-133b. Accordingly, miR-133b inhibited TAp63 expression through RhoA and its downstream pathways...
November 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27882171/role-of-microrna-141-in-colorectal-cancer-with-lymph-node-metastasis
#7
Li Feng, Hongqing Ma, Liang Chang, Xinliang Zhou, Na Wang, Lianmei Zhao, Jing Zuo, Yudong Wang, Jing Han, Guiying Wang
The present study aimed to investigate the role of microRNA (miR)-141 in the pathogenesis of colorectal cancer (CRC). In total, 58 CRC patients were included in the present study. The mRNA and protein expression levels of mitogen-activated protein kinase 4 (MAP4K4) were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. The miRNA-141 expression was measured by RT-qPCR, while serum MAP4K4 content was detected by enzyme-linked immunosorbent assay...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27878288/microrna-6826-and-6875-in-plasma-are-valuable-non%C3%A2-invasive-biomarkers-that-predict-the-efficacy-of-vaccine-treatment-against-metastatic-colorectal-cancer
#8
Taiki Kijima, Shoichi Hazama, Ryouichi Tsunedomi, Hironori Tanaka, Hiroko Takenouchi, Shinsuke Kanekiyo, Yuka Inoue, Masao Nakashima, Michihisa Iida, Kazuhiko Sakamoto, Nobuaki Suzuki, Shigeru Takeda, Tomio Ueno, Shigeru Yamamoto, Shigefumi Yoshino, Kiyotaka Okuno, Hiroaki Nagano
Various vaccine treatments against metastatic colorectal cancer have been developed and applied. However, to improve the efficacy of immunotherapy, biomarkers that can predict the effects are needed. It has been reported that various microRNAs (miRNAs) in peripheral blood may be useful as non-invasive biomarkers. In this study, miRNAs influencing the efficacy of vaccine treatment were screened for in a microarray analysis of 13 plasma samples that were obtained from patients prior to vaccine treatment. To validate the screening results, real-time RT-PCR was performed using 93 plasma samples obtained from patients prior to vaccine treatment...
November 22, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27876571/fusobacterium-nucleatum-increases-proliferation-of-colorectal-cancer-cells-and-tumor-development-in-mice-by-activating-tlr4-signaling-to-nf%C3%AE%C2%BAb-upregulating-expression-of-microrna-21
#9
Yongzhi Yang, Wenhao Weng, Junjie Peng, Leiming Hong, Lei Yang, Yuji Toiyama, Renyuan Gao, Minfeng Liu, Mingming Yin, Cheng Pan, Hao Li, Bomin Guo, Qingchao Zhu, Qing Wei, Mary-Pat Moyer, Ping Wang, Sanjun Cai, Ajay Goel, Huanlong Qin, Yanlei Ma
BACKGROUND & AIMS: Nearly 20% of the global cancer burden can be linked to infectious agents. Fusobacterium nucleatum promotes tumor formation by epithelial cells via unclear mechanisms. We aimed to identify microRNAs (miRNAs) induced by F nucleatum and evaluate their ability to promote colorectal carcinogenesis in mice. METHODS: Colorectal cancer (CRC) cell lines were incubated with F nucleatum or control reagents and analyzed in proliferation and would healing assays...
November 19, 2016: Gastroenterology
https://www.readbyqxmd.com/read/27861461/microrna-455-3p-inhibits-tumor-cell-proliferation-and-induces-apoptosis-in-hct116-human-colon-cancer-cells
#10
Jiantao Zheng, Zhenlv Lin, Lin Zhang, Hui Chen
BACKGROUND MicroRNAs have been reported to play significant roles in pathogenesis of colorectal cancer (CRC). In the present study, we aimed to investigate the functional role of microRNA-455-3p (miR-455-3p) in CRC, as well as its underlying mechanisms. MATERIAL AND METHODS Human colon cancer cell line HCT116 cells were transfected with miR-455-3p mimics, inhibitors, or controls. After transfection, the effects of miR-455-3p mimics or inhibitors on cell proliferation were analyzed by 3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl -2-H-tetrazolium bromide (MTT) assay and BrdU assay, and the effects of miR-455-3p mimics or inhibitors on cell apoptosis were determined...
November 18, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27859935/single-nucleotide-polymorphisms-within-micrornas-microrna-targets-and-microrna-biogenesis-genes-and-their-impact-on-colorectal-cancer-survival
#11
Lila E Mullany, Jennifer S Herrick, Roger K Wolff, Martha L Slattery
We have shown that single nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, miRNA target genes, and miRNA biogenesis genes minimally contribute to colon cancer risk. It is possible that these SNPs alter survival. We analyzed 565 SNPs in or adjacent to microRNAs, target genes, or biogenesis genes, using 1115 cases and 1173 controls; 837 cases had survival information. We tested SNPs for associations with colorectal cancer (CRC) survival using a Cox proportional hazard model adjusting for age, study center, gender, AJCC disease stage, and MSI tumor status...
November 18, 2016: Genes, Chromosomes & Cancer
https://www.readbyqxmd.com/read/27855613/hoxb7-and-hsa-mir-222-as-the-potential-therapeutic-candidates-for-metastatic-colorectal-cancer
#12
Maryam Iman, Seyede Samaneh Mostafavi, Seyed Shahriar Arab, Sadegh Azimzadeh, Mansour Poorebrahim
BACKGROUND: Recent studies have shown that the high mortality of patients with colorectal cancer (CRC) is related to its ability to spread the surrounding tissues, thus there is a need for designing and developing new drugs. OBJECTIVE: Here, we proposed a combinational therapy strategy, an inhibitory peptide in combination with miRNA targeting, for modulating CRC metastasis. In this study, some of the recent patents were also reviewed. METHODS: After data analysis with GEO2R and gene annotation using DAVID server, regulatory interactions of differentially expressed genes (DEGs) were obtained from STRING, GeneMANIA, KEGG and TRED databases...
2016: Recent Patents on Anti-cancer Drug Discovery
https://www.readbyqxmd.com/read/27853382/genetic-variation-rs7930-in-the-mir-4273-5p-target-site-is-associated-with-a-risk-of-colorectal-cancer
#13
Ah-Reum Lee, Jongkeun Park, Keum Ji Jung, Sun Ha Jee, Sungjoo Kim-Yoon
PURPOSE: MicroRNAs (miRNAs) are noncoding RNAs that play roles as tumor suppressors or oncogenes by regulating the expression of target genes via binding to seed-match sequences. Polymorphisms in the miRNA-binding site of a target gene can alter miRNA binding and potentially affect the risk of cancer. The objective of this study was to identify single-nucleotide polymorphisms (SNPs) in miRNA-binding sites and assess their involvement in the risk of colorectal cancer (CRC). MATERIALS AND METHODS: SNPs in the 3' untranslated regions of genes were selected and assessed for their effects on CRC risk in Korean population using participants in Korean Cancer Prevention Study-II...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27842582/leptin-and-insulin-up-regulate-mir-4443-to-suppress-ncoa1-and-traf4-and-decrease-the-invasiveness-of-human-colon-cancer-cells
#14
Ari Meerson, Hila Yehuda
BACKGROUND: Obesity is a risk factor for colorectal cancer (CRC). Normal and tumor cells respond to metabolic hormones, such as leptin and insulin. Thus, obesity-associated resistance to these hormones likely leads to changes in gene expression and behavior of tumor cells. However, the mechanisms affected by leptin and insulin signaling in CRC cells remain mostly unknown. METHODS: We hypothesized that microRNAs (miRNAs) are involved in the regulation of tumorigenesis-related gene expression in CRC cells by leptin and insulin...
November 14, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27827523/microrna-143-inhibits-colorectal-cancer-cell-proliferation-through-targeting-mmp7
#15
Bing Yu, Xuezhong Liu, Hong Chang
BACKGROUND: MicroRNAs (miRNAs) play critical roles in the development and progression of human malignancy. MiR-143 as a tumor suppressor, is decreased in malignant tumors, including colorectal cancer (CRC). However, the potential mechanism of miR-143 in CRC remains largely unknown. METHODS: Target prediction programs and luciferase reporter assay was used to predict the targets of miR-143. Following overexpression of miR-143 in CRC cells, target gene matrix metallopeptidase 7(MMP7) expression was detected by quantitative real-time PCR (qRT-PCR) and western blot...
November 9, 2016: Minerva Medica
https://www.readbyqxmd.com/read/27803053/polymorphisms-in-microrna-binding-sites-of-mucin-genes-as-predictors-of-clinical-outcome-in-colorectal-cancer-patients
#16
Veronika Vymetalkova, Barbara Pardini, Fabio Rosa, Katerina Jiraskova, Cornelia Di Gaetano, Petra Bendova, Miloslav Levy, Veronika Veskrnova, Tomas Buchler, Ludmila Vodickova, Alessio Naccarati, Pavel Vodicka
Polymorphisms in microRNA (miRNA) binding sites may affect miRNA/target gene interaction, resulting in differential mRNA/protein expression and susceptibility to common diseases. Mucins have been identified as markers of adverse prognosis. We hypothesized that genetic variations in miRNA binding sites located in mucin genes may modulate signaling response and the maintenance of genomic stability ultimately affecting cancer susceptibility, efficacy of chemotherapy and survival.In the present study, we analyzed the association of single nucleotide polymorphisms in predicted miRNA target sites (miRSNPs) of mucin genes with colorectal cancer (CRC) risk and clinical outcome...
November 1, 2016: Carcinogenesis
https://www.readbyqxmd.com/read/27802194/screening-of-exosomal-micrornas-from-colorectal-cancer-cells
#17
Cillian Clancy, Sonja Khan, Claire L Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James A L Brown, Myles R Joyce, Michael J Kerin, Roisin M Dwyer
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown. OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs. METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines...
September 30, 2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/27798879/microrna-324-in-human-cancer-mir-324-5p-and-mir-324-3p-have-distinct-biological-functions-in-human-cancer
#18
Wei-Ting Kuo, Shou-Yu Yu, Sung-Chou Li, Hing-Chung Lam, Hong-Tai Chang, Wei-Shone Chen, Chung-Yu Yeh, Syue-Fen Hung, Tsai-Chi Liu, Tony Wu, Chia-Cheng Yu, Kuo-Wang Tsai
MicroRNAs (miRNAs) are small non-coding RNAs that have crucial regulatory functions in carcinogenesis. miR-324-5p and miR-324-3p are generated from the same hairpin RNA structure, however, both are diverse in their direct target genes and expression levels. We report that expression of miR-324-5p and -3p was frequently observed to be either up-regulated or down-regulated, and the selection preference of miR-324 for 5p and 3p arms significantly varied in various types or human cancer. Overexpression of miR-324-5p or -3p suppressed growth and invasion of breast cancer cells...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27784183/investigating-mirna-mrna-regulatory-networks-using-crosslinking-immunoprecipitation-methods-for-biomarker-and-target-discovery-in-cancer
#19
Mireia Mato Prado, Adam E Frampton, Elisa Giovannetti, Justin Stebbing, Leandro Castellano, Jonathan Krell
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Recently, different experimental approaches, such as RNA Sequencing, crosslinking immunoprecipitation (CLIP) methods and its variations, together with computational approaches have been developed to elucidate the miRNA-mRNA targetome. Areas covered: This report focuses on comparing the different experimental and computational approaches, describing their advantages and disadvantages and providing several examples of preclinical (in vitro and in vivo) and clinical studies that have identified miRNA target genes in various tumour types, including breast, ovary, colorectal and pancreas...
November 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27780077/association-of-cigarette-smoking-and-microrna-expression-in-rectal-cancer-insight-into-tumor-phenotype
#20
Lila E Mullany, Jennifer S Herrick, Roger K Wolff, John R Stevens, Martha L Slattery
Smoking is known to influence messenger RNA (mRNA) expression in colorectal cancer (CRC) cases. As microRNAs (miRNAs) are known repressors of mRNAs, we hypothesize that smoking may influence miRNA expression, thus altering mRNA expression. Our sample consisted of 1447 CRC cases that had normal colorectal mucosa and carcinoma miRNA data and lifestyle data. We examined current smoking, current versus never and former versus never (C/F/N) smoking(1), and pack-years smoked with miRNA expression in normal mucosa as well as differential miRNA expression between paired normal and carcinoma tissue for colon and rectal tissue to determine associations between smoking and miRNA expression...
December 2016: Cancer Epidemiology
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