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miRNA, microRNA, Colorectal cancer

Likun Yan, Jianfeng Yao, Jian Qiu
microRNAs (miRNAs) have been reported to play crucial roles in malignant tumor progression, including cell development, proliferation, and progression. Increasing evidence suggests that miR-495 functions as either an oncogene or tumor suppressor in several tumor types. However, its biological role in the development of colorectal cancer (CRC) still remains unclear. In this study, we found that miR-495 expression level was remarkably down-regulated in CRC tissues samples and cell lines when compared to adjacent normal tissues and cell line by using qRT-PCR detection...
December 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Monika Stachowiak, Tatiana Flisikowska, Stefan Bauersachs, Carolin Perleberg, Hubert Pausch, Marek Switonski, Alexander Kind, Dieter Saur, Angelika Schnieke, Krzysztof Flisikowski
MicroRNAs are dysregulated in various cancers including colorectal cancer, and are potential useful biomarkers of disease development. We used next generation sequencing to investigate miRNA expression profiles in low- and high-grade intraepithelial dysplastic polyps from pigs carrying a mutation in the adenomatous polyposis coli tumour suppressor (APC1311 , orthologous to human APC1309 ) that model an inherited predisposition to colorectal cancer, familial adenomatous polyposis. We identified several miRNAs and their isomiRs significantly (P < 0...
November 10, 2017: Oncotarget
Xiang Jin, Yinghui Guan, Hui Sheng, Yang Liu
We investigated the transcriptional mechanism underlying lung cancer development. RNA sequencing analysis was performed on blood samples from lung cancer cases and healthy controls. Differentially expressed microRNAs (miRNAs), circular RNAs (circRNAs), mRNAs (genes), and long non-coding RNAs (lncRNA) were identified, followed by pathway enrichment analysis. Based on miRNA target interactions, a competing endogenous network was established and significant nodes were screened. Differentially expressed transcriptional factors were retrieved from the TRRUST database and the transcriptional factor regulatory network was constructed...
October 31, 2017: Oncotarget
Jian Zhao, Jian Xu, Rui Zhang
Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer-related mortality worldwide. Aberrant expression of miRNAs play important roles in the development and progression of various types of cancers by modulating oncogenic and tumour-suppressor pathways. Therefore, exploring the functions of microRNAs (miRNAs) that specifically contribute to CRC tumourigenesis and tumour development would greatly aid in obtaining more information on CRC and provide new targets for its diagnosis and treatment...
December 5, 2017: Oncology Reports
Shushan Yan, Zengfang Wang, Quanhong Duan, Changcheng Wang, Donghua Xu
Many studies have implicated that microRNAs (miRNAs), as non-coding RNAs, play important roles in the development and progression of colorectal cancer (CRC). However, little is known about the role of a newly identified miRNA, miR-6869-5p, in CRC. We aim to investigate the modifying effects and underlying mechanisms of miR-6869-5 in colorectal carcinogenesis and progression. Significantly reduced levels of miR-6869-5p were observed in both serum exosomes tumor tissue samples from patients with CRC. The prediction of targets of miR-6869-5p in databases of targetscan, microRNA...
December 5, 2017: Journal of Cellular Physiology
H W He, N N Wang, X M Yi, C P Tang, D Wang
BACKGROUND: colorectal cancer (CRC) is the second leading cause of cancer and cancer-related death in the world. Noninvasive biomarkers for early diagnosis of CRC are highly demanded. OBJECTIVE: The up-regulation of specific microRNAs (miRNAs) in serum has been considered a promising biomarker of CRC and miR-24-2 may be a potential biomarker in the diagnosis the progression of CRC. METHODS: Sixty-eighty healthy subjects and 228 CRC patients were divided into six groups: control group, CRC 0, CRC I, CRC II, CRC III, CRC IV and CRC V...
November 17, 2017: Cancer Biomarkers: Section A of Disease Markers
Gaurav Gupta, Dinesh K Chellappan, Terezinha de Jesus Andreoli Pinto, Philip M Hansbro, Mary Bebawy, Kamal Dua
MicroRNAs (miRNAs) are noncoding RNAs of around 20-25 nucleotides in length with highly conserved characteristics. They moderate posttranscriptional silencing by precisely combining with 3' untranslated regions (UTRs) of target mRNAs at a complementary site. miR503, an associate of the "canonical" miRNA16 family, is expressed in numerous types of tumors such as breast cancer, prostate cancer, lung cancer, colorectal cancer, hepatocellular carcinoma, glioblastoma and several others. There is convincing evidence to show that miR503 functions as a tumor suppressor gene through its effects on target genes that regulate cell proliferation, migration, and invasion in tumor cells...
November 22, 2017: Panminerva Medica
Shuo Chen, Yan Wang, Lin Zhang, Yinan Su, Mingqing Zhang, Juan Wang, Xipeng Zhang
The aim of the present study was to investigate the mechanism of metastasis in colorectal cancer (CRC) using microRNA (miRNA) and mRNA expression profiles. The mRNA and miRNA expression profiles of the GSE2509 and GSE56350 datasets were obtained from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were identified using the limma software package. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs...
December 2017: Oncology Letters
Yanjie Xu, Jianping Chen, Cao Gao, Danxia Zhu, Xiaoli Xu, Changping Wu, Jingting Jiang
MicroRNAs (miRNAs) have been demonstrated to serve an important role in diverse biological processes and cancer progression. Downregulation of microRNA-497 (miR-497) has been observed in human colorectal cancer (CRC) tissues, but the function of miR-497 in CRC has not been well investigated. In the present study, it was demonstrated that expression of miR-497 was significantly downregulated in human CRC tissues compared to adjacent normal tissues. Enforced expression of miR-497 inhibited proliferation, migration and invasion abilities of CRC cell lines SW1116 and SW480...
December 2017: Oncology Letters
Yongfu Xiong, Rong Wang, Linglong Peng, Wenxian You, Jinlai Wei, Shouru Zhang, Xingye Wu, Jinbao Guo, Jun Xu, Zhenbing Lv, Zhongxue Fu
Although the outcome of patients with colorectal cancer (CRC) has improved significantly, prognosis evaluation still presents challenges due to the disease heterogeneity. Increasing evidences revealed the close correlation between aberrant expression of certain RNAs and the prognosis. We envisioned that combined multiple types of RNAs into a single classifier could improve postoperative risk classification and add prognostic value to the current stage system. Firstly, differentially expressed RNAs including mRNAs, miRNAs and lncRNAs were identified by two different algorithms...
October 17, 2017: Oncotarget
Naghmeh Shirafkan, Behzad Mansoori, Ali Mohammadi, Navid Shomali, Mehri Ghasbi, Behzad Baradaran
Colorectal cancer is one of the most prevalent cancers with high mortality in the world. MicroRNAs are a class of small non-coding RNAs that regulate gene expression through targeting mRNAs. MicroRNAs involve in many biological and pathological processes such as cell growth, differentiation, apoptosis. etc. Dysregulation of miRNAs expression patterns have been reported in many tumors including Colorectal Cancer. Various studies indicate that miRNAs can be utilized as diagnostic and prognostic biomarkers for evaluation of tumor initiation, development, invasion, metastasis and response to chemotherapeutic drugs...
November 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Liang Huang, Jin Lin Cai, Pin Zhu Huang, Liang Kang, Mei Jin Huang, Lei Wang, Jian Ping Wang
MicroRNAs (miRNAs) are widely up-regulated or down-regulated in a variety of tumors, including lung cancer, liver cancer, and colorectal cancer (CRC). Furthermore, miRNAs can function as tumor suppressors or proto-oncogenes by controlling the growth and metastasis of cancer cells. In the present study, we found a significant increase in miR19b-3p levels in CRC compared to tumor tissue and revealed the role of miR19b-3p in CRC growth and metastasis. The exogenous overexpression of miR19b-3p induced the proliferation, migration, and invasion of CRC cells in vitro...
2017: American Journal of Cancer Research
Hidekazu Takahashi, Masanobu Takahashi, Shinobu Ohnuma, Michiaki Unno, Yuki Yoshino, Kota Ouchi, Shin Takahashi, Yasuhide Yamada, Hideki Shimodaira, Chikashi Ishioka
BACKGROUND: The aim of this study was to identify miRNAs specifically dysregulated in BRAF-mutated colorectal cancer, which could lead to a better understanding of the molecular mechanisms underlying oncogenesis of this malignant subtype of colorectal cancer. METHODS: Candidate dysregulated miRNAs were selected in genome-wide miRNA expression array analysis using a screening set composed of 15 BRAF-mutated and 15 non-KRAS/BRAF-mutated colorectal cancers. The miRNA expressions were validated in another set of patients...
November 7, 2017: BMC Cancer
Jin Ke, Weiwei Shao, Yasu Jiang, Junfei Xu, Feng Li, Jun Qin
Given the emerging role of microRNAs (miRs) in cancer progression, the present study investigated the role and underlying mechanism of miR‑103 in colorectal cancer (CRC). Reverse transcription‑quantitative polymerase chain reaction was conducted to quantify the expression levels of miR‑103 in clinical specimens and cell lines. The role of miR‑103 in CRC was examined using MTT, colony formation and transwell assays. In addition, a luciferase reporter assay was used to confirm an associated between the 3' untranslated region of zonula occuldens‑1 (ZO‑1) and miR‑103...
November 7, 2017: Molecular Medicine Reports
Jinhua Zhang, Gottumakkala S Raju, David W Chang, Shu-Hong Lin, Zhinan Chen, Xifeng Wu
BACKGROUND: Circulating microRNAs (miRNAs) are emerging as promising biomarkers for cancer. The objective of the current study was to investigate the potential of circulating cell-free miRNAs as biomarkers for colorectal cancer (CRC) and its precursor lesion, colorectal adenoma. METHODS: The serum levels of 800 miRNAs were assessed in a discovery set of 21 patients with CRC, 19 patients with adenoma, and 21 healthy controls using the NanoString miRNA analysis platform...
November 7, 2017: Cancer
Laura Moody, Hongshan He, Yuan-Xiang Pan, Hong Chen
The screening and diagnosis of colorectal cancer (CRC) currently relies heavily on invasive endoscopic techniques as well as imaging and antigen detection tools. More accessible and reliable biomarkers are necessary for early detection in order to expedite treatment and improve patient outcomes. Recent studies have indicated that levels of specific microRNA (miRNA) are altered in CRC; however, measuring miRNA in biological samples has proven difficult, given the complicated and lengthy PCR-based procedures used by most laboratories...
2017: Clinical Epigenetics
Chongtae Kim, Youlim Hong, Heejin Lee, Hoin Kang, Eun Kyung Lee
Multidrug resistance is one major barrier to successful chemotherapy. Although several studies have attempted to overcome resistance of cancer cells to anti-cancer drugs, key determinants of resistance remain largely unknown. The objective of this study was to investigate whether microRNAs might play a role in the acquisition of resistance. Human colorectal cancer HCT-116 cell lines were transduced with a lentivirus library containing 578 precursor microRNAs (miRNAs) to establish cell lines resistant to 5-fluorouracil (5-FU)...
November 3, 2017: Cancer Letters
Baohuan Li, Miaomiao Huang, Meiying Liu, Shiling Wen, Fang Sun
Colorectal cancer (CRC) is the third most common type of diagnosed cancer and the fourth leading cause of cancer‑associated mortalities worldwide. Increasing studies have demonstrated that the deregulation of microRNAs (miRNAs or miRs) is associated with the occurrence and development of multiple types of human cancer, including CRC. miR‑329 has been identified to be downregulated in various types of cancer; however, its expression pattern, functions and mechanisms in CRC remain unclear. The present study demonstrated that miR‑329 was lowly expressed in CRC tissue samples and cell lines...
October 2017: Molecular Medicine Reports
Shirley James, Jayasekharan S Aparna, Aswathy Mary Paul, Manendra Babu Lankadasari, Sabira Mohammed, Valsalakumari S Binu, Thankayyan R Santhoshkumar, Girijadevi Reshmi, Kuzhuvelil B Harikumar
Colorectal cancer is currently the third leading cause of cancer related deaths. There is considerable interest in using dietary intervention strategies to prevent chronic diseases including cancer. Cardamonin is a spice derived nutraceutical and herein, for the first time we evaluated the therapeutic benefits of cardamonin in Azoxymethane (AOM) induced mouse model of colorectal cancer. Mice were divided into 4 groups of which three groups were given six weekly injections of AOM. One group served as untreated control and remaining groups were treated with either vehicle or Cardamonin starting from the same day or 16 weeks after the first AOM injection...
October 24, 2017: Scientific Reports
Mai Ashizawa, Hirokazu Okayama, Aung Kyi Thar Min, Masaru Noda, Keita Aoto, Takahiro Nakajima, Teruhide Ishigame, Kousaku Mimura, Koji Kono
Programmed cell death 1(PD-1)/PD-ligand 1(PD-L1)immune checkpoint blockade has emerged as a promising therapeutic strategy in various types of cancer. In a recent phase II clinical trial, treatment with the anti-PD-1 agent, pembrolizumab, resulted in considerable clinical benefit in patients with mismatch repair(MMR)-deficient colorectal cancer(CRC). Upregulation of PD-1on T-cells and PD-L1 on tumor cells induces inhibitory signals to suppress T-cell activation, leading to an immune-suppressive microenvironment particularly in MMR-deficient tumors...
October 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
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