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miRNA, microRNA, Colorectal cancer

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https://www.readbyqxmd.com/read/28092102/micrornas-potential-candidates-for-diagnosis-and-treatment-of-colorectal-cancer
#1
REVIEW
Abdullah Moridikia, Hamed Mirzaei, Amirhossein Sahebkar, Jafar Salimian
Colorectal cancer (CRC) is known as the third common cancer worldwide and an important public health problem in different populations. Several genetics and environmental risk factors are involved in the development and progression of CRC including chromosomal abnormalities, epigenetic alterations, and unhealthy lifestyle. Identification of risk factors and biomarkers could lead to a better understanding of molecular pathways involved in CRC pathogenesis. MicroRNAs (miRNAs) are important regulatory molecules which could affect a variety of cellular and molecular targets in CRC...
January 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#2
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28072746/znf148-modulates-top2a-expression-and-cell-proliferation-via-cerna-regulatory-mechanism-in-colorectal-cancer
#3
Xian Hua Gao, Juan Li, Yan Liu, Qi Zhi Liu, Li Qiang Hao, Lian Jie Liu, Wei Zhang
BACKGROUND: Competing endogenous RNA (ceRNA) regulation is a novel hypothesized mechanism that states RNA molecules share common target microRNAs (miRNAs) and may competitively combine into the same miRNA pool. METHODS: Zinc finger protein 148 (ZNF148) and TOP2A expression were analyzed in 742 colorectal cancer (CRC) tissues using immunohistochemistry (IHC). ZNF148 mRNA, TOP2A mRNA, miR101, miR144, miR335, and miR365 expression were estimated in 53 fresh frozen CRC tissues by reverse transcription polymerase chain reaction...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28054337/combined-mirna-profiling-and-proteomics-demonstrates-that-different-mirnas-target-a-common-set-of-proteins-to-promote-colorectal-cancer-metastasis
#4
Sofía Torres, Irene Garcia-Palmero, Rubén A Bartolomé, María Jesús Fernandez-Aceñero, Elena Molina, Eva Calviño, Miguel F Segura, J Ignacio Casal
The process of liver colonisation in colorectal cancer remains poorly characterised. Here, we addressed the role of microRNA (miRNA) dysregulation in metastasis. We first compared miRNA expression profiles between colorectal cancer cell lines with different metastatic properties and then identified target proteins of the dysregulated miRNAs to establish their functions and prognostic value. We found that 38 miRNAs were differentially expressed between highly metastatic (KM12SM/SW620) and poorly metastatic (KM12C/SW480) cancer cell lines...
January 5, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28053552/diet-and-lifestyle-factors-associated-with-mirna-expression-in-colorectal-tissue
#5
Martha L Slattery, Jennifer S Herrick, Lila E Mullany, John R Stevens, Roger K Wolff
MicroRNAs (miRNAs) are small non-protein-coding RNA molecules that regulate gene expression. Diet and lifestyle factors have been hypothesized to be involved in the regulation of miRNA expression. In this study it was hypothesized that diet and lifestyle factors are associated with miRNA expression. Data from 1,447 cases of colorectal cancer to evaluate 34 diet and lifestyle variables using miRNA expression in normal colorectal mucosa as well as for differential expression between paired carcinoma and normal tissue were used...
2017: Pharmacogenomics and Personalized Medicine
https://www.readbyqxmd.com/read/28039449/expression-analysis-of-microrna-as-prognostic-biomarkers-in-colorectal-cancer
#6
Jie Yang, Dongling Ma, Andrew Fesler, Haiyan Zhai, Apisri Leamniramit, Wenzhe Li, Song Wu, Jingfang Ju
microRNA (miRNA) based biomarkers have unique advantages due to their critical regulatory function, superior stability, and relatively small number compared to mRNAs. A number of miRNAs play key roles in colon cancer stem cell chemoresistance and have clinical potential as prognostic biomarkers. The purpose of this study is to systematically validate the prognostic potential of miRNAs in colorectal cancer. In this study, we validated the prognostic potential of a panel of miRNAs using 205 stage II, III, and IV colorectal cancer specimens by qRT-PCR analysis...
December 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/28036302/high-expression-of-mir-181c-as-a-predictive-marker-of-recurrence-in-stage-ii-colorectal-cancer
#7
Nobuyoshi Yamazaki, Yoshikatsu Koga, Hirokazu Taniguchi, Motohiro Kojima, Yukihide Kanemitsu, Norio Saito, Yasuhiro Matsumura
INTRODUCTION: A standard treatment for stage II colorectal cancer (CRC) is surgical resection without adjuvant chemotherapy. However, the recurrence rate of these patients is approximately 20%. To date, there are no robust biomarkers suitable for predicting recurrence in stage II CRC patients. In this study, microRNAs (miRNAs) extracted from CRC tissues were examined for a possible biomarker to predict recurrence in stage II CRC patients. RESULTS: From the comprehensive analysis, 15 miRNAs were selected as candidates for further study...
December 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/28032929/downregulation-of-mir-100-mir-125b-is-associated-with-lymph-node-metastasis-in-early-colorectal-cancer-with-submucosal-invasion
#8
Yasuteru Fujino, Shunsaku Takeishi, Kensei Nishida, Koichi Okamoto, Naoki Muguruma, Tetsuo Kimura, Shinji Kitamura, Hiroshi Miyamoto, Akiko Fujimoto, Jun Higashijima, Mitsuo Shimada, Kazuhito Rokutan, Tetsuji Takayama
A majority of early colorectal cancers (CRCs) with submucosal invasion undergo surgical operation despite very low incidence of lymph node metastasis. Our study aimed to identify microRNAs (miRNAs) specifically responsible for lymph node metastasis in submucosal CRCs. MicroRNA microarray analysis revealed that miR-100 and miR-125b expression levels were significantly lower in CRC tissues with lymph node metastases than in those without metastases. These results were validated by quantitative real-time PCR in a larger set of clinical samples...
December 29, 2016: Cancer Science
https://www.readbyqxmd.com/read/28004114/mir-20a-directed-regulation-of-bid-is-associated-with-the-trail-sensitivity-in-colorectal-cancer
#9
Guanli Huang, Xiangjian Chen, Yefeng Cai, Xiaobo Wang, Chungen Xing
MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in cancer processes. Although miR-20a has been reported to be altered in a range of cancers, the role of miR-20a in colorectal cancer is not fully characterized, and the relationship between miR-20a dysregulation and the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sensitivity is not defined. In the present study, we demonstrated significant upregulation of miR-20a in the serum of colorectal cancer patients, tumor tissues and cell lines by quantitative RT-PCR analysis...
January 2017: Oncology Reports
https://www.readbyqxmd.com/read/28000854/identification-of-microrna-487b-as-a-negative-regulator-of-liver-metastasis-by-regulation-of-kras-in-colorectal-cancer
#10
Tsuyoshi Hata, Yukako Mokutani, Hidekazu Takahashi, Akira Inoue, Koji Munakata, Kazuya Nagata, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Kohei Murata, Tsunekazu Mizushima, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto
Recent studies have shown that microRNAs (miRNAs) are involved in the progression of colorectal cancer (CRC). The aim of this study is to identify a novel miRNA that especially relates to liver metastasis and to explore the underlying mechanism. Differentially expressed miRNAs were analyzed using microarray, in primary CRC tumors without metastasis (n=16), those with liver metastasis (n=12), and liver metastatic lesions (n=8). We found that miR-487b level decreased in liver metastatic lesions, and qRT-PCR confirmed the results in the validating cohort (n=134)...
December 20, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27991929/mir-19a-promotes-colitis-associated-colorectal-cancer-by-regulating-tumor-necrosis-factor-alpha-induced-protein-3-nf-%C3%AE%C2%BAb-feedback-loops
#11
T Wang, X Xu, Q Xu, J Ren, S Shen, C Fan, Y Hou
Chronic inflammation is believed to have a crucial role in colon cancer development. MicroRNA (miRNA) deregulation is common in human colorectal cancers, but little is known regarding whether miRNA drives tumor progression by regulating inflammation. Here, we showed that miR-19a can promote colitis and colitis-associated colon cancer (CAC) development using a CAC mouse model and an acute colitis mouse model. Tumor necrosis factor-α (TNF-α) stimulation can increase miR-19a expression, and upregulated miR-19a can in turn activate nuclear factor (NF)-κB signaling and TNF-α production by targeting TNF alpha-induced protein 3 (TNFAIP3)...
December 19, 2016: Oncogene
https://www.readbyqxmd.com/read/27982722/targetlink-a-new-method-for-identifying-the-endogenous-target-set-of-a-specific-microrna-in-intact-living-cells
#12
Yan Xu, Yan Chen, Daliang Li, Qing Liu, Zhenyu Xuan, Wen-Hong Li
MicroRNAs are small non-coding RNAs acting as posttranscriptional repressors of gene expression. Identifying mRNA targets of a given miRNA remains an outstanding challenge in the field. We have developed a new experimental approach, TargetLink, that applied locked nucleic acid (LNA) as the affinity probe to enrich target genes of a specific microRNA in intact cells. TargetLink also consists a rigorous and systematic data analysis pipeline to identify target genes by comparing LNA-enriched sequences between experimental and control samples...
December 16, 2016: RNA Biology
https://www.readbyqxmd.com/read/27935861/microrna-203-predicts-human-survival-after-resection-of-colorectal-liver-metastasis
#13
T Peter Kingham, Hoang C B Nguyen, Jian Zheng, Ioannis T Konstantinidis, Eran Sadot, Jinru Shia, Deborah Kuk, Steven Zhang, Leonard Saltz, Michael I D'Angelica, William R Jarnagin, Hani Goodarzi, Sohail F Tavazoie
BACKGROUND: Resection of colorectal liver metastasis (CRLM) can be curative. Predicting which patients may benefit from resection, however, remains challenging. Some microRNAs (miRNAs) become deregulated in cancers and contribute to cancer progression. We hypothesized that miRNA expression can serve as a prognostic marker of survival after CRLM resection. RESULTS: MiR-203 was significantly overexpressed in tumors of short-term survivors compared to long-term survivors...
December 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27922690/microrna-152-inhibits-tumor-cell-growth-by-directly-targeting-rtkn-in-hepatocellular-carcinoma
#14
Jiejing Zhou, Yanjun Zhang, Yuhong Qi, Dequan Yu, Qiuju Shao, Jun Liang
Hepatocellular carcinoma (HCC) is the most common form of adult liver cancer and accounts for approximately 90% of all cases of primary liver cancer annually. Rhotekin (RTKN), which functions as a cancer promoter, can be frequently detected in many human cancers, including gastric cancer, colorectal carcinoma and bladder carcinoma. The aim of this study was to investigate the role of RTKN in HCC. Using HCC cells and tissues from patients with liver cancer, we demonstrated that RTKN was significantly increased in HCC...
December 5, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27916938/comprehensive-analysis-of-mirnome-alterations-in-response-to-sorafenib-treatment-in-colorectal-cancer-cells
#15
Anna-Maria Pehserl, Anna Lena Ress, Stefanie Stanzer, Margit Resel, Michael Karbiener, Elke Stadelmeyer, Verena Stiegelbauer, Armin Gerger, Christian Mayr, Marcel Scheideler, Georg C Hutterer, Thomas Bauernhofer, Tobias Kiesslich, Martin Pichler
MicroRNAs (miRNAs) are master regulators of drug resistance and have been previously proposed as potential biomarkers for the prediction of therapeutic response in colorectal cancer (CRC). Sorafenib, a multi-kinase inhibitor which has been approved for the treatment of liver, renal and thyroid cancer, is currently being studied as a monotherapy in selected molecular subtypes or in combination with other drugs in metastatic CRC. In this study, we explored sorafenib-induced cellular effects in Kirsten rat sarcoma viral oncogene homolog olog (KRAS) wild-type and KRAS-mutated CRC cell lines (Caco-2 and HRT-18), and finally profiled expression changes of specific miRNAs within the miRNome (>1000 human miRNAs) after exposure to sorafenib...
December 1, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916606/fbx8-is-a-metastasis-suppressor-downstream-of-mir-223-and-targeting-mtor-for-degradation-in-colorectal-carcinoma
#16
F F Wang, X J Zhang, Y R Yan, X H Zhu, J Yu, Y Ding, J L Hu, W J Zhou, Z C Zeng, W T Liao, Y Q Ding, L Liang
F-box proteins are critical components of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases and involved in the ubiquitin-dependent proteolytic pathway. Dysregulation of F-box protein-mediated proteolysis often leads to human malignancies. F-box only protein 8 (FBX8), a novel component of F-box proteins, is down-regulated in several tumors and closely correlates with tumor progression. However, little is known about its function, regulatory mechanisms and substrates in the progression of colorectal carcinoma (CRC)...
December 1, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27906435/down-regulation-of-microrna-143-is-associated-with-colorectal-cancer-progression
#17
J-W Bai, H-Z Xue, C Zhang
OBJECTIVE: Colorectal cancer (CRC) is one of the most prevalent carcinomas worldwide. Tumor metastasis and recurrence are leading causes of CRC-related deaths. Given the role of microRNA (miRNA) in CRC invasion and metastasis, we explored the association between miRNA-143 expression and clinicopathologic characteristics in CRC, as well as the effects of miRNA-143 on CRC cell invasion in vitro. MATERIALS AND METHODS: Quantitative real-time PCR was conducted to assess the expression of miR-143 in tissue specimens and cell lines...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27904764/mir-193b-directly-targets-stmn1-and-inhibits-the-malignant-phenotype-in-colorectal-cancer
#18
Feng Guo, Yang Luo, Yi-Fei Mu, Shao-Lan Qin, Yang Qi, Yi-Er Qiu, Ming Zhong
Accumulating evidence suggests that aberrantly expressed microRNAs (miRNAs) contribute to the initiation and progression of human cancers. However, the underlying function of miR-193b in colorectal cancer (CRC) remains largely unexplored. Herein, we demonstrate that miR-193b is significantly down-regulated in CRC tissues compared with their normal counterparts. Kaplan-Meier analysis revealed that decreased miR-193b expression was closely associated with the shorter overall survival of patients with CRC. Through gain-and loss-of-function studies, we showed that miR-193b significantly suppressed CRC cell proliferation and invasion...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27903980/identification-of-biomarker-micrornas-for-predicting-the-response-of-colorectal-cancer-to-neoadjuvant-chemoradiotherapy-based-on-microrna-regulatory-network
#19
Yaqun Zhu, Qiliang Peng, Yuxin Lin, Li Zou, Peipei Shen, Feifei Chen, Ming Min, Li Shen, Jiajia Chen, Bairong Shen
Preoperative radiotherapy or chemoradiotherapy has become a standard procedure for treatment of patients with locally advanced colorectal cancer (CRC). However, patients' responses to treatment are different and personalized. MicroRNAs (miRNAs) are promising biomarkers for predicting personalized responses. In this study, we collected 30 publicly reported miRNAs associated with chemoradiotherapy of CRC. We extracted 46 differentially expressed miRNAs from samples of responders and non-responders to preoperative radiotherapy from the Gene Expression Omnibus dataset (Student's t test, p-value < 0...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27902469/the-mir-196b-mirna-inhibits-the-gata6-intestinal-transcription-factor-and-is-upregulated-in-colon-cancer-patients
#20
Sebastian Fantini, Valentina Salsi, Luca Reggiani, Antonino Maiorana, Vincenzo Zappavigna
OBJECTIVE: To explore the possible misexpression of the microRNA miR-196b in colorectal cancer (CRC) and its role in controlling the expression of GATA6, a putative target gene crucial to intestinal cell homeostasis and tumorigenesis. DESIGN: The expression of miR-196b was analysed by qRT-PCR in surgical resection samples from a cohort of sporadic colon cancer patients. Manipulations of miR-196b expression were performed to demonstrate its inhibition of GATA6 protein levels...
November 25, 2016: Oncotarget
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