keyword
MENU ▼
Read by QxMD icon Read
search

Lipid rafts

keyword
https://www.readbyqxmd.com/read/29792904/monoclonal-antibody-dl11c8-identifies-adam23-as-a-component-of-lipid-raft-microdomains
#1
Zaine L M Borgonovo, Caroline F Ribeiro, Michele D M Costa, Ingrid L M Souza, Gustavo R Rossi, Monica V Alcantara, Max Ingberman, Luciano G Braga, Adriana F Mercadante, Lia S Nakao, Silvio M Zanata
A disintegrin and metalloprotease protein 23 (ADAM23) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors. Here, we describe a novel monoclonal antibody (DL11C8) that specifically recognizes the cysteine-rich domain of both pre-protein (100kDa) and mature (70 kDa) forms of ADAM23 from different species, including human, rodents and avian orthologs...
May 21, 2018: Neuroscience
https://www.readbyqxmd.com/read/29783772/acid-sphingomyelinase-promotes-cellular-internalization-of-clostridium-perfringens-iota-toxin
#2
Masahiro Nagahama, Masaya Takehara, Kazuaki Miyamoto, Kazumi Ishidoh, Keiko Kobayashi
Clostridium perfringens iota-toxin is a binary actin-ADP-ribosylating toxin composed of the enzymatic component Ia and receptor binding component Ib. Ib binds to a cell surface receptor, forms Ib oligomer in lipid rafts, and associates with Ia. The Ia-Ib complex then internalizes by endocytosis. Here, we showed that acid sphingomyelinase (ASMase) facilitates the cellular uptake of iota-toxin. Inhibitions of ASMase and lysosomal exocytosis by respective blockers depressed cell rounding induced by iota-toxin...
May 20, 2018: Toxins
https://www.readbyqxmd.com/read/29782965/membrane-cholesterol-delays-cellular-apoptosis-induced-by-ginsenoside-rh2-a-steroid-saponin
#3
Sandrine L Verstraeten, Marie Albert, Adrien Paquot, Giulio G Muccioli, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq
Saponins exhibit several biological and pharmacological activities, such as antibacterial, anti-inflammatory and anticancer effects. Many studies attribute their activities to their interactions with cholesterol. In this study, we focus on the steroid saponin ginsenoside Rh2, one of the active principles of Panax ginseng root. Some evidence suggests that lipid rafts, defined as nanodomains enriched in cholesterol and sphingolipids, could be involved in the Rh2-induced apoptosis. However, the role of membrane lipids, especially cholesterol, in this process is still poorly understood...
May 18, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29780974/metformin-targets-glucose-metabolism-in-triple-negative-breast-cancer
#4
R S Wahdan-Alaswad, S M Edgerton, H S Salem, A D Thor
Metformin is the most widely administered anti-diabetic agent worldwide. In patients receiving metformin for metabolic syndrome or diabetes, it reduces the incidence and improves the survival of breast cancer (BC) patients. We have previously shown that metformin is particularly potent against triple negative breast cancer (TNBC), with a reduction of proliferation, oncogenicity and motility, inhibition of pro-oncogenic signaling pathways and induction of apoptosis. These BCs are well recognized to be highly dependent on glucose/glucosamine (metabolized through anaerobic glycolysis) and lipids, which are metabolized for the production of energy and cellular building blocks to sustain a high rate of proliferation...
2018: Journal of Oncology Translational Research
https://www.readbyqxmd.com/read/29772704/lateral-membrane-heterogeneity-regulates-viral-induced-membrane-fusion-during-hiv-entry
#5
Rodion J Molotkovsky, Veronika V Alexandrova, Timur R Galimzyanov, Irene Jiménez-Munguía, Konstantin V Pavlov, Oleg V Batishchev, Sergey A Akimov
Sphingomyelin- and cholesterol- enriched membrane domains, commonly referred to as "rafts" play a crucial role in a large number of intra- and intercellular processes. Recent experiments suggest that not only the volumetric inhomogeneity of lipid distribution in rafts, but also the arrangement of the 1D boundary between the raft and the surrounding membrane is important for the membrane-associated processes. The reason is that the boundary preferentially recruits different peptides, such as HIV (human immunodeficiency virus) fusion peptide...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29767519/molecular-structure-and-permeability-at-the-interface-between-phase-separated-membrane-domains
#6
Rodrigo Maghdissian Cordeiro
Phase-separated membrane domains, also known as lipid rafts, are believed to play an important role in cell function. Although most rafts are sterol-enriched membrane regions, evidence suggests that living cells may also contain gel-like rafts. Interactions between gel and fluid domains have a large impact on membrane properties, as is the case with permeability. The membrane permeability may reach a peak at the main phase transition temperature, by far exceeding the values recorded at the fluid phase. It has been proposed that gel-fluid interfaces are leaky, but the effect has not yet been demonstrated at the molecular level...
May 16, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29761896/sphingolipids-regulate-neuromuscular-synapse-structure-and-function-in-drosophila
#7
Ryan J H West, Laura Briggs, Maria Perona Fjeldstad, Richard R Ribchester, Sean T Sweeney
Sphingolipids are found in abundance at synapses and have been implicated in regulation of synapse structure, function and degeneration. Their precise role in these processes, however, remains obscure. Serine Palmitoyl-transferase (SPT) is the first enzymatic step for synthesis of sphingolipids. Analysis of the Drosophila larval neuromuscular junction revealed mutations in the SPT enzyme subunit, lace/SPTLC2 resulted in deficits in synaptic structure and function. Although neuromuscular junction (NMJ) length is normal in lace mutants, the number of boutons per NMJ is reduced to ∼50% of the wild type number...
May 15, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29757947/membrane-remodeling-as-a-key-player-of-the-hepatotoxicity-induced-by-co-exposure-to-benzo-a-pyrene-and-ethanol-of-obese-zebrafish-larvae
#8
Muhammad Imran, Odile Sergent, Arnaud Tête, Isabelle Gallais, Martine Chevanne, Dominique Lagadic-Gossmann, Normand Podechard
The rise in prevalence of non-alcoholic fatty liver disease (NAFLD) constitutes an important public health concern worldwide. Including obesity, numerous risk factors of NAFLD such as benzo[a]pyrene (B[a]P) and ethanol have been identified as modifying the physicochemical properties of the plasma membrane in vitro thus causing membrane remodeling-changes in membrane fluidity and lipid-raft characteristics. In this study, the possible involvement of membrane remodeling in the in vivo progression of steatosis to a steatohepatitis-like state upon co-exposure to B[a]P and ethanol was tested in obese zebrafish larvae...
May 14, 2018: Biomolecules
https://www.readbyqxmd.com/read/29754714/membrane-potential-distinctly-modulates-mobility-and-signaling-of-il-2-and-15-receptors-in-t-cells
#9
Éva Nagy, Gábor Mocsár, Veronika Sebestyén, Julianna Volkó, Ferenc Papp, Katalin Tóth, Sándor Damjanovich, György Panyi, Thomas A Waldmann, Andrea Bodnár, György Vámosi
The high electric field across the plasma membrane might influence the conformation and behavior of transmembrane proteins that have uneven charge distributions in or near their transmembrane regions. Membrane depolarization of T cells occurs in the tumor microenvironment and in inflamed tissues because of K+ release from necrotic cells and hypoxia affecting the expression of K+ channels. However, little attention has been given to the effect of membrane potential (MP) changes on membrane receptor function...
May 10, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29752898/dynamic-pattern-generation-in-cell-membranes-current-insights-into-membrane-organization
#10
REVIEW
Krishnan Raghunathan, Anne K Kenworthy
It has been two decades since the lipid raft hypothesis was first presented. Even today, whether these nanoscale cholesterol-rich domains are present in cell membranes is not completely resolved. However, especially in the last few years, a rich body of literature has demonstrated both the presence and the importance of non-random distribution of biomolecules on the membrane, which is the focus of this review. These new developments have pushed the experimental limits of detection and have brought us closer to observing lipid domains in the plasma membrane of live cells...
May 9, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29751779/essential-role-of-atp6ap2-enrichment-in-caveolae-lipid-raft-microdomains-for-the-induction-of-neuronal-differentiation-of-stem-cells
#11
Nehman Makdissy, Katia Haddad, Jeanne D'arc AlBacha, Diana Chaker, Bassel Ismail, Albert Azar, Ghada Oreibi, David Ayoub, Ibrahim Achkar, Didier Quilliot, Ziad Fajloun
BACKGROUND: The subcellular distribution of prorenin receptor and adaptor protein ATP6AP2 may affect neurogenesis. In this study, we hypothesized that ATP6AP2 expression and subcellular relocalization from caveolae/lipid raft microdomains (CLR-Ms) to intracellular sites may correlate with neuronal differentiation (Neu-Dif) of adipose-derived mesenchymal stem cells (ADSCs). METHODS: Human ADSCs isolated from 24 healthy donors and 24 patients with neurological disorders (ND) were cultured and induced for Neu-Dif...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29748690/untargeted-lipidomic-analysis-of-primary-human-epidermal-melanocytes-acutely-and-chronically-exposed-to-uv-radiation
#12
Núria Dalmau, Nathalie Andrieu-Abadie, Romà Tauler, Carmen Bedia
Ultraviolet (UV) radiation present in sunlight has been related to harmful effects on skin such as premature aging and skin cancer. In order to study the effects of UV radiation on skin, many investigations have been carried out at transcriptomic and proteomic levels. However, studies on the effects of UV radiation on lipid composition are scarce. In this work, primary cultures of melanocytes were exposed to UV radiation in a similar UVA/UVB ratio to that found in solar light. The UV exposure was carried out twice a week and different endpoints were investigated at 0...
May 11, 2018: Molecular omics
https://www.readbyqxmd.com/read/29747817/gangliosides-in-inflammation-and-neurodegeneration
#13
Koichi Furukawa, Yuhsuke Ohmi, Orie Tajima, Yuki Ohkawa, Yuji Kondo, Ji Shuting, Noboru Hashimoto, Keiko Furukawa
Gangliosides play roles in the regulation of cell signaling that are mediated via membrane microdomains, lipid rafts. In this review, functions of gangliosides in the maintenance of nervous systems with a focus on regulation of inflammation and neurodegeneration are addressed. During analyses of various ganglioside-lacking mutant mice, we demonstrated that nervous tissues exhibited inflammatory reactions and subsequent neurodegeneration. Among inflammation-related genes, factors of the complement system showed up-regulation with aging...
2018: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29746928/cholesterol-inhibits-hepatocellular-carcinoma-invasion-and-metastasis-by-promoting-cd44-localization-in-lipid-rafts
#14
Zhishi Yang, Wenhao Qin, Yao Chen, Bo Yuan, Xiaoling Song, Bibo Wang, Feng Shen, Jing Fu, Hongyang Wang
Cholesterol plays a vital role in modulating the action of membrane proteins critical to cellular function. The effect of serum cholesterol on the prognosis of hepatocellular carcinoma (HCC) patients remains uncertain. Here, we report that high levels of cholesterol predict good survival and low disease recurrence after surgery. Cholesterol could significantly suppress migration and invasion of HCC cells and restrain metastasis of HCC in mice. High levels of cholesterol promoted CD44 translocation into lipid rafts and attenuated CD44-Ezrin binding, which are crucial for cell migration and cancer metastasis...
May 7, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29740315/compartmentalized-camp-signaling-associated-with-lipid-raft-and-non-raft-membrane-domains-in-adult-ventricular-myocytes
#15
Shailesh R Agarwal, Jackson Gratwohl, Mia Cozad, Pei-Chi Yang, Colleen E Clancy, Robert D Harvey
Aim: Confining cAMP production to discrete subcellular locations makes it possible for this ubiquitous second messenger to elicit unique functional responses. Yet, factors that determine how and where the production of this diffusible signaling molecule occurs are incompletely understood. The fluid mosaic model originally proposed that signal transduction occurs through random interactions between proteins diffusing freely throughout the plasma membrane. However, it is now known that the movement of membrane proteins is restricted, suggesting that the plasma membrane is segregated into distinct microdomains where different signaling proteins can be concentrated...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29733910/the-contribution-of-cholesterol-and-epigenetic-changes-to-the-pathophysiology-of-breast-cancer
#16
REVIEW
Maliha T Munir, Christopher Ponce, Catherine A Powell, Kaiser Tarafdar, Teru Yanagita, Mahua Choudhury, Lauren S Gollahon, Shaikh M Rahman
Breast cancer is one of the most commonly diagnosed cancers in women. Accumulating evidence suggests that cholesterol plays an important role in the development of breast cancer. Even though the mechanistic link between these two factors is not well understood, one possibility is that dysregulated cholesterol metabolism may affect lipid raft and membrane fluidity and can promote tumor development. Current studies have shown oxysterol 27-hydroxycholesterol (27-HC) as a critical regulator of cholesterol and breast cancer pathogenesis...
May 4, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29730243/docosahexaenoic-acid-regulates-the-formation-of-lipid-rafts-a-unified-view-from-experiment-and-simulation
#17
Stephen R Wassall, Xiaoling Leng, Samuel W Canner, Edward Ross Pennington, Jacob J Kinnun, Andres T Cavazos, Sahil Dadoo, Dylan Johnson, Frederick A Heberle, John Katsaras, Saame Raza Shaikh
Docosahexaenoic acid (DHA, 22:6) is an n-3 polyunsaturated fatty acid (n-3 PUFA) that influences immunological, metabolic, and neurological responses through complex mechanisms. One structural mechanism by which DHA exerts its biological effects is through its ability to modify the physical organization of plasma membrane signaling assemblies known as sphingomyelin/cholesterol (SM/chol)-enriched lipid rafts. Here we studied how DHA acyl chains esterified in the sn-2 position of phosphatidylcholine (PC) regulate the formation of raft and non-raft domains in mixtures with SM and chol on differing size scales...
May 3, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29728594/roles-of-galnac-disialyl-lactotetraosyl-antigens-in-renal-cancer-cells
#18
Akiko Tsuchida, Motohiro Senda, Akihiro Ito, Seiichi Saito, Makoto Kiso, Takayuki Ando, Anne Harduin-Lepers, Akio Matsuda, Keiko Furukawa, Koichi Furukawa
GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin...
May 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29727452/antigen-b-from-echinococcus-granulosus-enters-mammalian-cells-by-endocytic-pathways
#19
Edileuza Danieli da Silva, Martin Cancela, Karina Mariante Monteiro, Henrique Bunselmeyer Ferreira, Arnaldo Zaha
BACKGROUND: Cystic hydatid disease is a zoonosis caused by the larval stage (hydatid) of Echinococcus granulosus (Cestoda, Taeniidae). The hydatid develops in the viscera of intermediate host as a unilocular structure filled by the hydatid fluid, which contains parasitic excretory/secretory products. The lipoprotein Antigen B (AgB) is the major component of E. granulosus metacestode hydatid fluid. Functionally, AgB has been implicated in immunomodulation and lipid transport. However, the mechanisms underlying AgB functions are not completely known...
May 4, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29725363/overexpression-of-csk-binding-protein-phosphoprotein-associated-with-glycosphingolipid-enriched-microdomains-induces-cluster-of-differentiation-59-mediated-apoptosis-in-jurkat-cells
#20
Bei-Bei Cong, Mei-Hua Gao, Bing Li, Bing Wang, Bei Zhang, Li-Na Wang, Shu-Chao Zhang, Hua-Qiao Li, Zhong Wang, Shu-Yi Han
Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains (CBP/PAG) is a membrane-bound adaptor protein that downregulates the activation of Src family kinases present in lipid rafts. To elucidate the role of CBP/PAG in human T cell activation, a cell line overexpressing CBP/PAG was constructed and the function of CBP/PAG in Jurkat cells was examined. The present study revealed that increased CBP/PAG expression in T cells significantly enhanced their apoptosis and reduced cellular activation and proliferation...
May 2018: Experimental and Therapeutic Medicine
keyword
keyword
60627
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"