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https://www.readbyqxmd.com/read/29793031/cloning-overexpression-and-purification-of-a-gene-of-unknown-function-of-prophage-loci-from-candidatus-liberibacter-asiaticus-the-destructive-bacterial-pathogen-of-huanglongbing-disease-in-citrus-plants
#1
Duangtip Sudhan, Thamrongjet Puttamuk, Supachai Vuttipongchaikij, Pitak Chuawong
Citrus Huanglongbing (HLB) or citrus greening is one of the most destructive diseases affecting citrus industry worldwide. The causal agent in Asia is a phloem-limited, Gram-negative bacterium, 'Candidatus Liberibacter asiaticus' (CLas). Within the genome of CLas lies prophage regions, classified as Type-A, B, C, and D. In particular, Type-D has been indicated to correlate with the blotchy-mottle symptoms of citrus trees. Here we reported the cloning, overexpression, and purification of the ORF1, an open reading frame from the partial Type-D region of CLas obtained from an infected lime tree (Citrus aurantifolia Swingle)...
May 21, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29792928/translation-of-dipeptide-repeat-proteins-from-the-c9orf72-expanded-repeat-is-associated-with-cellular-stress
#2
Yoshifumi Sonobe, Ghanashyam Ghadge, Katsuhisa Masaki, Ataman Sendoel, Elaine Fuchs, Raymond P Roos
Expansion of a hexanucleotide repeat (HRE), GGGGCC, in the C9ORF72 gene is recognized as the most common cause of familial amyotrophic lateral sclerosis (FALS), frontotemporal dementia (FTD) and ALS-FTD, as well as 5-10% of sporadic ALS. Despite the location of the HRE in the non-coding region (with respect to the main C9ORF72 gene product), dipeptide repeat proteins (DPRs) that are thought to be toxic are translated from the HRE in all three reading frames from both the sense and antisense transcript. Here, we identified a CUG translation initiation codon that has a good Kozak consensus sequence as the translation initiation codon...
May 21, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29791619/temporal-ordering-and-reading-competence-of-words-and-pseudowords-a-preliminary-study
#3
Cintia Alves de Souza, Andrezza Gonzalez Escarce, Stela Maris Aguiar Lemos
Purpose Analyze the association of simple and complex auditory temporal ordering skills with reading competence tasks in students. Methods Pilot phase of a cross-sectional study conducted with a non-probabilistic sample of 22 schoolchildren aged eight to ten years. Instruments for evaluation of reading competence tasks and simple and complex auditory temporal ordering abilities were used. Results Most participants obtained adequate results in the tests of simple auditory temporal ordering (SATO) and reading competence (RC), but inadequate results in the test of complex auditory temporal ordering (CATO)...
2018: CoDAS
https://www.readbyqxmd.com/read/29789182/geodermatophilus-chilensis-sp-nov-from-soil-of-the-yungay-core-region-of-the-atacama-desert-chile
#4
Jean Franco Castro, Imen Nouioui, Vartul Sangal, Martha E Trujillo, Maria Del Carmen Montero-Calasanz, Tara Rahmani, Alan T Bull, Juan A Asenjo, Barbara A Andrews, Michael Goodfellow
A polyphasic study was undertaken to establish the taxonomic status of three representative Geodermatophilus strains isolated from an extreme hyper-arid Atacama Desert soil. The strains, isolates B12T , B20 and B25, were found to have chemotaxonomic and morphological properties characteristic of the genus Geodermatophilus. The isolates shared a broad range of chemotaxonomic, cultural and physiological features, formed a well-supported branch in the Geodermatophilus 16S rRNA gene tree in which they were most closely associated with the type strain of Geodermatophilus obscurus...
April 4, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29788376/the-ocean-gene-atlas-exploring-the-biogeography-of-plankton-genes-online
#5
Emilie Villar, Thomas Vannier, Caroline Vernette, Magali Lescot, Miguelangel Cuenca, Aurélien Alexandre, Paul Bachelerie, Thomas Rosnet, Eric Pelletier, Shinichi Sunagawa, Pascal Hingamp
The Ocean Gene Atlas is a web service to explore the biogeography of genes from marine planktonic organisms. It allows users to query protein or nucleotide sequences against global ocean reference gene catalogs. With just one click, the abundance and location of target sequences are visualized on world maps as well as their taxonomic distribution. Interactive results panels allow for adjusting cutoffs for alignment quality and displaying the abundances of genes in the context of environmental features (temperature, nutrients, etc...
May 21, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29788136/an-experimental-evaluation-of-the-benefits-and-costs-of-providing-fertility-information-to-adolescents-and-emerging-adults
#6
J Boivin, E Koert, T Harris, L O'Shea, A Perryman, K Parker, C Harrison
STUDY QUESTION: Does the provision of fertility (compared to control) information affect fertility-related knowledge, perceived threat of infertility, anxiety, physical stress and fertility plans in adolescents and emerging adults? SUMMARY ANSWER: The provision of fertility information was associated with increased fertility knowledge (emerging adults) and greater infertility threat (adolescents and emerging adults). WHAT IS KNOWN ALREADY: According to fertility education research, adolescents and emerging adults know less than they should know about fertility topics...
May 17, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29786122/complete-genomic-sequence-of-a-novel-macluravirus-alpinia-oxyphylla-mosaic-virus-alomv-identified-in-alpinia-oxyphylla
#7
Weiyao Hu, Zengping Li, Xiaoye Wang, Wenbo Liu, Chaohao Huang, Weiguo Miao, Hongguang Cui
A macluravirus, tentatively named alpinia oxyphylla mosaic virus (AloMV), was identified in Alpinia oxyphylla, and its complete genomic sequence determined. The positively single-stranded RNA genome is comprised of 8213 nucleotides excluding the poly (A) tail, and contains one large open reading frame encoding a polyprotein of 2,626 amino acids. Blastp search showed that the polyprotein of AloMV shared 48%~68% aa sequence identities with other reported macluraviruses. Phylogenetic analysis based on the nucleotide sequence of the polyprotein showed that AloMV, together with all other macluraviruses, clustered into the same group most closely related to cardamom mosaic virus, sharing 66...
May 21, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29785971/community-noise-exposure-and-annoyance-activity-interference-and-academic-achievement-among-university-students
#8
Rattapon Onchang, Darryl W Hawker
Background: Noise annoyance and effects on academic performance have been investigated for primary and secondary school students but comparatively little work has been conducted with university students who generally spend more time in dormitories or accommodation for their self-study. Objective: To determine, using a socio-acoustic approach involving face-to-face interviews and actual noise measurements, the effect of various community noise sources on student activities in accommodation both inside and outside a university precinct and also relationships with cumulative grade point average (GPA)...
May 2018: Noise & Health
https://www.readbyqxmd.com/read/29785656/how-to-measure-the-immunosuppressive-activity-of-mdsc-assays-problems-and-potential-solutions
#9
Annika M Bruger, Anca Dorhoi, Gunes Esendagli, Katarzyna Barczyk-Kahlert, Pierre van der Bruggen, Marie Lipoldova, Tomas Perecko, Juan Santibanez, Margarida Saraiva, Jo A Van Ginderachter, Sven Brandau
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization...
May 21, 2018: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/29785401/rheumatoid-arthritis-and-mirnas-a-critical-review-through-a-functional-view
#10
REVIEW
Maria Cristina Moran-Moguel, Stefania Petarra-Del Rio, Evangelina E Mayorquin-Galvan, Maria G Zavala-Cerna
Rheumatoid arthritis (RA) is a systemic autoimmune disease with severe joint inflammation and destruction associated with an inflammatory environment. The etiology behind RA remains to be elucidated; most updated concepts include the participation of environmental, proteomic, epigenetic, and genetic factors. Epigenetic is considered the missing link to explain genetic diversification among RA patients. Within epigenetic factors participating in RA, miRNAs are defined as small noncoding molecules with a length of approximately 22 nucleotides, capable of gene expression modulation, either negatively through inhibition of translation and degradation of the mRNA or positively through increasing the translation rate...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/29785169/self-reported-functional-communicative-and-critical-health-literacy-on-foodborne-diseases-in-accra-ghana
#11
Sangeeta Gupta, Raymond Asare Tutu, John Boateng, Janice Desire Busingye, Sathya Elavarthi
Background: Although substantial progress has been made in reducing total mortality resulting from foodborne diseases, diarrheal illness are still the second most common illnesses among children. In Ghana, foodborne diseases have consistently been among the top 20 causes of outpatient illness over the last couple of decades. This study, therefore, examines health literacy on foodborne diseases and the relative effects of health literacy on self-rated health. Methods: Foodborne diseases are major causes of morbidity and mortality globally...
2018: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29784842/eucast-determination-of-olorofim-f901318-susceptibility-of-mould-species-method-validation-and-mics
#12
Karin Meinike Jørgensen, Karen M T Astvad, Rasmus Krøger Hare, Maiken Cavling Arendrup
Olorofim is a novel antifungal agent with in vitro activity against Aspergillus and some other moulds. Here we addressed technical aspects for EUCAST olorofim testing and generated contemporary MIC data.EUCAST E.Def 9.3.1 testing was performed comparing two plate preparation methods (serial-dilution in medium (serial-plates) versus pre-dilution in DMSO (ISO-plates)), two lots of olorofim, visual (visual-MIC) versus spectrophotometer (spec-MIC) reading and four polystyrene plates using 34-53 Aspergillus isolates from five genera...
May 21, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29783982/providing-japanese-health-care-information-for-international-visitors-digital-animation-intervention
#13
Mariko Nishikawa, Masaaki Yamanaka, Junko Kiriya, Masamine Jimba
BACKGROUND: Over 24 million international visitors came to Japan in 2016 and the number is expected to increase. Visitors could be at a risk of illness or injury that may result in hospitalization in Japan. We assessed the effects of a four-minute digital animation titled Mari Info Japan on the level of anxiety experienced by international visitors to Japan. METHODS: We conducted a non-randomized, controlled study at Narita International Airport outside Tokyo in December 2014...
May 21, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29783016/systematic-investigation-of-the-erigeron-breviscapus-mechanism-for-treating-cerebrovascular-disease
#14
Jiangmei Wang, Yangyang Chen, Baoshi Liu, Qian Wang, Lulu Zhang, Zhenzhong Wang, Jun Zhou, Wei Xiao, Chunli Zheng, Yonghua Wang
ETHNOPHARMACOLOGICAL RELEVANCE: Cerebrovascular diseases (CBVDs), characterized by striking morbidity and mortality, have become the most common life-threatening diseases. The existing drugs of CBVDs target one or a few of pathogenic factors, the efficacy of which is limited because of the complexity of CBVDs. Traditional Chinese medicine (TCM), featured by multi-component and multi-target endows the great effectiveness in CBVDs treatment. For instance, Erigeron breviscapus (vant.) Hand...
May 18, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29781685/detection-referral-and-control-of-diabetes-and-hypertension-in-the-rural-eastern-cape-province-of-south-africa-by-community-health-outreach-workers-in-the-rural-primary-healthcare-project-health-in-every-hut
#15
Angela A Morris-Paxton, Paul Rheeder, Rose-Marie G Ewing, Dillon Woods
BACKGROUND:  Non-communicable diseases, mainly cardiovascular diseases, diabetes, cancer and chronic respiratory diseases, are responsible for approximately 63% of all deaths occurring worldwide in any given year. The majority of these deaths have occurred in low- and middle-income countries (LMICs). The latest World Health Organization (WHO) report shows that the increase in diabetes is also most pronounced in the LMICs. The South African Labour and Development Research Unit estimated a 9% prevalence within the adult population in 2016...
April 11, 2018: African Journal of Primary Health Care & Family Medicine
https://www.readbyqxmd.com/read/29780584/opening-the-black-box-of-spring-water-microbiology-from-alpine-karst-aquifers-to-support-proactive-drinking-water-resource-management
#16
REVIEW
Domenico Savio, Philipp Stadler, Georg H Reischer, Alexander K T Kirschner, Katalin Demeter, Rita Linke, Alfred P Blaschke, Regina Sommer, Ulrich Szewzyk, Inés C Wilhartitz, Robert L Mach, Hermann Stadler, Andreas H Farnleitner
Over the past 15 years, pioneering interdisciplinary research has been performed on the microbiology of hydrogeologically well-defined alpine karst springs located in the Northern Calcareous Alps (NCA) of Austria. This article gives an overview on these activities and links them to other relevant research. Results from the NCA springs and comparable sites revealed that spring water harbors abundant natural microbial communities even in aquifers with high water residence times and the absence of immediate surface influence...
May 2018: WIREs. Water
https://www.readbyqxmd.com/read/29780371/comparative-metatranscriptomics-of-wheat-rhizosphere-microbiomes-in-disease-suppressive-and-non-suppressive-soils-for-rhizoctonia-solani-ag8
#17
Helen L Hayden, Keith W Savin, Jenny Wadeson, Vadakattu V S R Gupta, Pauline M Mele
The soilborne fungus Rhizoctonia solani anastomosis group (AG) 8 is a major pathogen of grain crops resulting in substantial production losses. In the absence of resistant cultivars of wheat or barley, a sustainable and enduring method for disease control may lie in the enhancement of biological disease suppression. Evidence of effective biological control of R. solani AG8 through disease suppression has been well documented at our study site in Avon, South Australia. A comparative metatranscriptomic approach was applied to assess the taxonomic and functional characteristics of the rhizosphere microbiome of wheat plants grown in adjacent fields which are suppressive and non-suppressive to the plant pathogen R...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780362/characterization-and-genomic-study-of-phage-vb_ecos-b2-infecting-multidrug-resistant-escherichia-coli
#18
Yue Xu, Xinyan Yu, Yu Gu, Xu Huang, Genyan Liu, Xiaoqiu Liu
The potential of bacteriophage as an alternative antibacterial agent has been reconsidered for control of pathogenic bacteria due to the widespread occurrence of multi-drug resistance bacteria. More and more lytic phages have been isolated recently. In the present study, we isolated a lytic phage named vB_EcoS-B2 from waste water. VB_EcoS-B2 has an icosahedral symmetry head and a long tail without a contractile sheath, indicating that it belongs to the family Siphoviridae . The complete genome of vB_EcoS-B2 is composed of a circular double stranded DNA of 44,283 bp in length, with 54...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780339/your-co-author-received-150-citations-pride-but-not-envy-mediates-the-effect-of-system-generated-achievement-messages-on-motivation
#19
Sonja Utz, Nicole L Muscanell
ResearchGate, a social network site for academics, prominently displays the achievements of people one follows ("With 150 new reads, X was the most read author from their institute"). The goal of this paper was to examine the emotional and motivational effects of these system-generated messages, thereby extending prior research on envy-evoking status updates on Facebook to a professional context. We also extend the research on social comparisons and more broadly, on emotional responses elicited by social media...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/29780188/science-map-metaphors-a-comparison-of-network-versus-hexmap-based-visualizations
#20
Katy Börner, Adam H Simpson, Andreas Bueckle, Robert L Goldstone
Most maps of science use a network layout; few use a landscape metaphor. Human users are trained in reading geospatial maps, yet most have a hard time reading even simple networks. Prior work using general networks has shown that map-based visualizations increase recall accuracy of data. This paper reports the result of a comparison of two comparable renderings of the UCSD map of science that are: the original network layout and a novel hexmap that uses a landscape metaphor to layout the 554 subdisciplines grouped into 13 color-coded disciplines of science...
February 2018: Scientometrics
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