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https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#1
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in-vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In-vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
January 3, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29259455/human-male-infertility-and-its-genetic-causes
#2
REVIEW
Toshinobu Miyamoto, Gaku Minase, Takeshi Shin, Hiroto Ueda, Hiroshi Okada, Kazuo Sengoku
Background: Infertility affects about 15% of couples who wish to have children and half of these cases are associated with male factors. Genetic causes of azoospermia include chromosomal abnormalities, Y chromosome microdeletions, and specific mutations/deletions of several Y chromosome genes. Many researchers have analyzed genes in the AZF region on the Y chromosome; however, in 2003 the SYCP3 gene on chromosome 12 (12q23) was identified as causing azoospermia by meiotic arrest through a point mutation...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29247212/anti-mullerian-hormone-to-testosterone-ratio-is-predictive-of-positive-sperm-retrieval-in-men-with-idiopathic-non-obstructive-azoospermia
#3
Massimo Alfano, Eugenio Ventimiglia, Irene Locatelli, Paolo Capogrosso, Walter Cazzaniga, Filippo Pederzoli, Nicola Frego, Rayan Matloob, Antonino Saccà, Luca Pagliardini, Paola Viganò, Pietro Zerbi, Manuela Nebuloni, Marina Pontillo, Francesco Montorsi, Andrea Salonia
The lack of clinically-reliable biomarkers makes impossible to predict sperm retrieval outcomes at testicular sperm extraction (TESE) in men with non-obstructive azoospermia (NOA), resulting in up to 50% of unnecessary surgical interventions. Clinical data, hormonal profile and histological classification of testis parenchyma from 47 white-Caucasian idiopathic NOA (iNOA) men submitted to microdissection TESE (microTESE) were analyzed. Logistic regression analyses tested potential clinical predictors of positive sperm retrieval...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29243340/testicular-sperm-extraction-after-laparoscopic-orchiectomy-for-bilateral-postpubertal-intra-abdominal-cryptorchidism-what-chance-of-sperm-retrieval
#4
G Cito, P A Della Camera, S Degli Innocenti, M E Coccia, G Nesi, A Cocci, S Morselli, A Minervini, M Carini, M Serni, M Gacci, A Natali
Infertility occurs in up to 54% of men with bilateral undescended testes. Orchiectomy is considered the best therapeutic approach, especially when cryptorchidism is diagnosed in adulthood, due to a high risk of malignancy. A 33-year-old man was referred with a clinical presentation of empty scrotum and an ultrasonography and magnetic resonance imaging evaluation of intra-abdominal bilateral cryptorchidism. Follicle-stimulating hormone was 23.20 IU/L, luteinising hormone was 14.10 IU/L, total testosterone was 12...
December 15, 2017: Andrologia
https://www.readbyqxmd.com/read/29227563/expression-and-enzyme-activity-of-cytochrome-p450-enzymes-cyp3a4-and-cyp3a5-in-human-skin-and-tissue-engineered-skin-equivalents
#5
Sarah A Smith, Helen E Colley, Parveen Sharma, Klaudia M Slowik, Rowena Sison-Young, Andrew Sneddon, Steven D Webb, Craig Murdoch
CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue-engineered skin equivalents (TESE), and enzyme activity was detected using the model substrate benzyl-O-methyl-cyanocoumarin...
December 11, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/29184796/oncofertility-in-sarcoma-patients
#6
REVIEW
Diana M Lopategui, Raphael Yechieli, Ranjith Ramasamy
Treatment for sarcoma can significantly decrease fertility, both due to the irradiation of gonads, and the impact of chemotherapy on gametogenesis. Infertility in cancer survivors causes significant regret and decreased quality of life in their adulthood. As this cancer mainly affects children and young adults, fertility preservation is an essential part of survivorship care, however it remains one of the least implemented services in adolescent and young adult cancer patients. Success of fertility preservation is highly dependent on the referral prior to oncologic treatment...
October 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29179011/ivf-outcome-in-azoospermic-cancer-survivors
#7
S Dar, R Orvieto, J Levron, J Haas, Itai Gat, G Raviv
OBJECTIVE: To assess clinical outcome among infertile couples treated by in vitro fertilization (IVF) and intra cytoplasmic sperm injection (ICSI) using testicular sperm from azoospermic cancer survivors. STUDY DESIGN: This clinical retrospective study included infertile couples treated in a single tertiary referral center between 1996 and 2013. All male partners were cancer survivors who were diagnosed with azoospermia due to previous gonadotoxic treatments and referred to testicular sperm extraction (TESE)...
January 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29164757/considerations-in-fertility-preservation-in-cases-of-genital-trauma
#8
B Z Starmer, A Baird, M A Lucky
OBJECTIVE: To perform a review of the literature to assess the options of preserving fertility in patients with fertility threatening testicular injuries and their efficacy to help guide surgeons who encounter these patients in the future. METHODS: Medline, EMBASE and Cochrane library databases were searched using the keywords treatment, therapy, management, scrotal trauma / injury, testicular trauma / injury / amputation, fertility and fertility preservation Inclusion criteria: studies reporting the fertility preserving techniques with a history of testicular trauma with loss of one, both or nearly all testicular parenchymal tissue RESULTS: Two cases of testicular sperm extraction, eight cases of testicular replantation, and one case of cryopreservation after injury were identified...
November 22, 2017: BJU International
https://www.readbyqxmd.com/read/29150736/effect-of-erythrocyte-sperm-separation-medium-on-nuclear-acrosomal-and-membrane-maturity-parameters-in-human-sperm
#9
Bikem Soygur, Soner Celik, Ciler Celik-Ozenci, Leyla Sati
PURPOSE: The purpose of this study is to investigate whether erythrocyte-sperm separation medium (ESSM) has effects on human sperm motility, morphology, viability, membrane maturity, acrosome integrity, and nuclear attributes before and after cryopreservation. METHODS: Semen samples from normozoospermic (n = 36) and oligozoospermic (n = 9) patients were analyzed. Samples from the same patient were divided into three aliquots: group 1 and group 2 were resuspended in sperm washing media and ESSM, respectively...
November 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29094403/longitudinal-changes-in-lic-and-other-parameters-in-patients-receiving-different-chelation-regimens-data-from-licnet
#10
Angela Vitrano, Massimiliano Sacco, Rosamaria Rosso, Alessandra Quota, Daniela Fiorino, Esther Oliva, Calogera Gerardi, Gaetano Roccamo, Vincenzo Spadola, Aldo Filosa, Lorenzo Tesé, Giuseppina Calvaruso, Lorella Pitrolo, Laura Mistretta, Filippo Cassarà, Rosario Di Maggio, Aurelio Maggio
OBJECTIVES: The liver remains the primary site of iron storage, with liver iron concentration (LIC) being a strong surrogate of total body iron. MRI-R2 can accurately measure LIC. The LICNET (Liver Iron Cutino Net-work) was established to diagnostics of liver iron overload by MRI-R2 subjects with hemochromatosis in haematological disorders. The aims of the study were to look at variation of LIC measurements during time across different chelation regimens. METHODS: This was a cross-sectional study of 130 patients attending 9 Italian centres participating in the LICNET...
November 1, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/29090218/testicular-damage-following-testicular-sperm-retrieval-a-ram-model-study
#11
Jens Fedder, Niels Marcussen, Maja D K Fedder, Birte Engvad
The aim of this study was to evaluate the possible development of histological abnormalities such as fibrosis and microcalcifications after sperm retrieval in a ram model. Fourteen testicles in nine rams were exposed to open biopsy, multiple TESAs, or TESE, and the remaining four testicles were left unoperated on as controls. Three months after sperm retrieval, the testicles were removed, fixed, and cut into 1/2 cm thick slices and systematically put onto a glass plate exposing macroscopic abnormalities. Tissue from abnormal areas was cut into 3 μm sections and stained for histological evaluation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29064652/impact-of-testicular-sperm-extraction-and-testicular-sperm-aspiration-on-gonadal-function-in-an-experimental-rat-model
#12
Leocácio Venícius Sousa Barroso, Ricardo Reges, João Batista Gadelha Cerqueira, Eduardo P Miranda, Rafael Jorge Alves de Alcantara, Francisco Vagnaldo F Jamacaru, Manoel Odorico de Moraes, Maria Angelina da Silva Medeiros, Lúcio Flávio Gonzaga-Silva
PURPOSE: To assess the impact of sperm retrieval on the gonadal function of rats with impaired spermatogenesis by comparing testicular sperm extraction (TESE) to aspiration (TESA). The efficacy of these procedures to sperm obtainment was also compared. MATERIALS AND METHODS: A pilot study showed impaired spermatogenesis, but normal testosterone (T) production after a bilateral orchidopexy applied to 26 rats, which were randomly assigned into four groups: TESE (n=7), TESA (n=7), SHAM (n=6) and Control (n=6)...
October 24, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28992369/predictive-value-of-pre-operative-inflammation-based-prognostic-scores-neutrophil-to-lymphocyte-ratio-platelet-to-lymphocyte-ratio-and-monocyte-to-eosinophil-ratio-in-testicular-sperm-extraction-a-pilot-study
#13
C Yucel, M Z Keskin, O Cakmak, B Ergani, C Kose, O Celik, E Islamoglu, M Ucar, G Koc, Z Kozacioglu
To investigate the ability of some hematologic prognostic scores demonstrating inflammation in predicting sperm presence in testicular sperm extraction (TESE). We retrospectively investigated the medical data of 430 patients with the diagnosis of non-obstruc tive azoospermia (NOA) who had undergone TESE operation consecutively in our institution between the dates of January 2009 and February 2017. In all, 352 patients with the diagnosis of NOA, with bilaterally palpable vas deferens, who had undergone TESE for the first time, were included in the study...
October 9, 2017: Andrology
https://www.readbyqxmd.com/read/28990672/microdissection-testicular-sperm-extraction-in-finland-results-of-the-first-100-patients
#14
Rauni Klami, Harri Mankonen, Antti Perheentupa
INTRODUCTION: Testicular microdissection sperm extraction (MD-TESE) combined with intracytoplasmic sperm injection (ICSI) has made biological fatherhood possible for many men with the most severe form of male infertility, non-obstructive azoospermia. MD-TESE was introduced in Turku in 2008, and by 2015, 100 Finnish men with non-obstructive azoospermia have been operated on. MATERIAL AND METHODS: The average age was 33 years at the time of surgery. Forty-eight had a needle biopsy previously, 56% had testicular size less than 15ml...
October 9, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28974842/evaluation-of-prognostic-factors-and-determinants-in-surgical-sperm-retrieval-procedures-in-azoospermic-patients
#15
Hajrudin Spahovic, Ümit Göktolga, Dzelaludin Junuzovic, Cihan Göktaş, Admir Rama
INTRODUCTION: Main categorisation of azospermic is in two grups: nonobstructive azoospermia (NOA) and obstructive azoospermia (OA). We had evaluation of prognostic factors and determinants in sperm retrieval procedures in azoospermic patients. METHODS: Retrospective evaluation observed 21 selected patients with NOA and OA azoospermia, after that complete history, physical examination with ultarsound volume of testis and hormonal profile. Hormonal profile included: follicle stimulating hormone (FSH), luteinizing hormone (LH), testosteron (T) and prolactin (P) serum levels...
August 2017: Medical Archives
https://www.readbyqxmd.com/read/28904909/office-based-andrology-and-male-infertility-procedures-a-cost-effective-alternative
#16
Manaf Alom, Matthew Ziegelmann, Josh Savage, Tanner Miest, Tobias S Köhler, Landon Trost
BACKGROUND: From 2014-2016, our clinical practice progressively incorporated several male infertility and andrology procedures performed under local anesthesia, including circumcision, hydrocelectomy, malleable penile prostheses, orchiectomy, penile plication, spermatocelectomy, testicular prostheses, varicocelectomy, vasectomy reversal (VR), and testicular and microepididymal sperm aspiration (TESE/MESA). Given the observed outcomes and potential financial and logistical benefits of this approach for surgeons and patients, we sought to describe our initial experience...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28904906/a-step-by-step-guide-to-office-based-sperm-retrieval-for-obstructive-azoospermia
#17
REVIEW
Robert M Coward, Jesse N Mills
A variety of surgical options exists for sperm retrieval in the setting of obstructive azoospermia (OA). With appropriate preparation, the majority of these techniques can safely be performed in the office with local anesthesia and with or without monitored anesthesia care (MAC). The available techniques include percutaneous options such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm aspiration (TESA), as well as open techniques that include testicular sperm extraction (TESE) and microsurgical epididymal sperm aspiration (MESA)...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28868245/isolation-culture-and-characterization-of-human-sertoli-cells-by-flow-cytometry-development-of-procedure
#18
Mohammad Reza Lakpour, Samaneh Aghajanpour, Morteza Koruji, Abdolhossein Shahverdi, Mohammad Ali Sadighi-Gilani, Marjan Sabbaghian, Reza Aflatoonian, Majid Rajabian-Naghandar
BACKGROUND: The sertoli cells in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. These cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis. Given that the sertoli cells play an essential role in spermatogenesis and the lack of these cells leads to the disruption of spermatogenesis, it is necessary to investigate the behavior and performance of these cells...
April 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28866996/novosphingobium-ipomoeae-sp-nov-isolated-from-a-water-convolvulus-field
#19
Wen-Ming Chen, Cheng-Wen Huang, Jhen-Ci Chen, Zih-Han Chen, Shih-Yi Sheu
A bacterial strain designated Tese-5T was isolated from a water convolvulus field in Taiwan and characterized using the polyphasic taxonomic approach. Strain Tese-5T was an aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium and formed bright yellow coloured colonies. Strain Tese-5T grew at 15-35 °C (optimum, 30 °C), with 0-1.0 % NaCl (optimum, 0-0.5 %) and at pH 5.5-7 (optimum, pH 6). The major fatty acids (>10 %) of strain Tese-5T were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28853108/differences-in-poly-adp-ribose-polymerase1-parp1-and-proliferative-cell-nuclear-antigen-pcna-immunoreactivity-in-patients-who-experienced-successful-and-unsuccessful-microdissection-testicular-sperm-extraction-procedures
#20
Süleyman Akarsu, Baris Büke, Seren Gülsen Gürgen, Serkan Akdemir, Funda Gode, Merve Biçer, Mustafa Agah Tekindal, Ahmet Zeki Isik
PURPOSE: The aim of this study is to evaluate expression of deoxyribonucleic acid (DNA) synthesis and repair markers in testicular tissues of azoospermic men in whom sperm retrieval could and could not be achieved as a result of microdissection testicular sperm extraction (micro-TESE) procedure. MATERIALS AND METHODS: In this prospective cohort study, testicular tissues were retrieved from 60 Non-obstructive Azoospermia (NOA) patients who underwent micro-TESE procedure...
August 29, 2017: Urology Journal
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