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https://www.readbyqxmd.com/read/29785530/azoospermia-and-embryo-morphokinetics-testicular-sperm-derived-embryos-exhibit-delays-in-early-cell-cycle-events-and-increased-arrest-prior-to-compaction
#1
Nina Desai, Pavinder Gill, Nicholas N Tadros, Jeffrey M Goldberg, Edmund Sabanegh, Tommaso Falcone
PURPOSE: Sperm play an essential role in embryonic genome activation and embryonic progression to blastocyst. In the present work, we focus on development of embryos created as a result of ICSI with testicular or epididymal sperm from azoospermic males and compare this to outcomes from normospermic males. The objective of this study was to determine if sperm origin influences clinical outcomes, the kinetics of embryo development, or the incidence of cleavage anomalies and multinucleation...
May 21, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29745280/reporting-on-longitudinal-live-birth-rates-and-cumulative-delivery-rates-are-more-realistic-outcome-measures-than-sperm-retrieval-rates-in-couples-undergoing-mtese-icsi
#2
Khaled Almekaty, Saad Abomelha, Yau Thum, James Nicopoullos, Tim Bracewell-Milnes, Tet Yap, Suks Minhas
The live birth rates (LBRs) in men with non-obstructive azoospermia (NOA) undergoing microdissection TESE (mTESE) are under reported, with surgical sperm retrieval rate often used as the primary outcome measure. We analysed longitudinal and cumulative delivery rates following mTESE- intracytoplasmic sperm injection (ICSI) in NOA. Sperm was retrieved in 45% of patients undergoing mTESE. Median male and female age were 42 years (IQR 37-47) and 35 years (IQR 31-38) respectively. A total of 141 ICSI cycles and 23 frozen embryo transfers resulted in 62 pregnancies and 37 LBRs...
May 10, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29738176/-microdissection-testicular-sperm-extraction-for-patients-with-nonmosaic-klinefelter-syndrome-a-report-of-143-cases
#3
Jia-Ming Mao, Lian-Ming Zhao, Kai Hong, Wen-Hao Tang, De-Feng Liu, Hao-Cheng Lin, Han Wu, Hai-Tao Zhang, Hong-Liang Zhang, Yu-Zhuo Yang, Ya-Peng Wang, Ying Lian, Hui Jiang, Lu-Lin Ma, Jie Qiao
Objective: To investigate the clinical application and outcomes of microdissection testicular sperm extraction (micro-TESE) in patients with nonmosaic Klinefelter syndrome (KS). METHODS: A total of 143 nonmosaic KS patients underwent micro-TESE in the Center of Reproductive Medicine of Peking University Third Hospital between July 2012 and August 2016. We analyzed their clinical and follow-up data and evaluated the outcomes. RESULTS: Spermatozoa were successfully retrieved from the testicular tissue in 44...
December 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29726895/the-risk-of-tese-induced-hypogonadism-a-systematic-review-and-meta-analysis
#4
Jitske Eliveld, Madelon van Wely, Andreas Meißner, Sjoerd Repping, Fulco van der Veen, Ans M M van Pelt
BACKGROUND: Testicular sperm extraction (TESE) is a surgical procedure to retrieve spermatozoa from the testes of men with azoospermia to help them achieve biological parenthood. Although effective, the surgical procedure is not without complications and haematoma, devascularization, inflammation and a decrease in testosterone levels have been described as such. The prevalence and duration of hypogonadism and associated symptoms after TESE have not been studied systematically. OBJECTIVE AND RATIONALE: In this systematic review we addressed the following research questions: Are serum testosterone levels decreased after TESE and, if so, do these levels recover over time? What is the prevalence of symptoms and signs related to hypogonadism after TESE and are they related to testosterone levels? SEARCH METHODS: We searched the databases Pubmed and Embase from 1 January 1993 to 26 June 2017...
May 3, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29723455/-impacts-of-different-procedures-of-testicular-sperm-retrieval-on-testicular-function-and-antisperm-antibodies-in-azoospermia-patients
#5
Yang-Yang Hu, Li-Yuan Wang, Bo-Tian Song, Shun-Shun Cao, Ao-Lei Chen
Objective: To investigate the influence of different procedures of testicular sperm retrieval on the levels of serum inhibin B (INHB), antisperm antibodies (AsAb), follicle-stimulating hormone (FSH), and testosterone (T) in patients with azoospermia. METHODS: We randomly assigned 210 azoospermia patients to receive testicular sperm extraction (TESE, n = 50), testicular sperm aspiration (TESA, n = 56), testicular fine needle aspiration (TEFNA, n = 64), or microscopic TESE (micro-TESE, n = 40)...
July 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29713543/advances-in-sperm-retrieval-techniques-in-azoospermic-men-a-systematic-review
#6
Rupin Shah, Chirag Gupta
Objective: To evaluate various methods of operative sperm retrieval in men with non-obstructive azoospermia (NOA) and to determine the optimal surgical approach in terms of effectiveness, morbidity, and complications. Materials and methods: PubMed and Cochrane databases were searched to identify five recent reviews and meta-analyses evaluating outcomes for sperm retrieval in men with NOA. Results and Conclusion: Micro-TESE is the most efficient method for retrieving sperm but requires special expertise and can be traumatic for the testes...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29707937/detection-of-y-chromosome-microdeletions-and-hormonal-profile-analysis-of-infertile-men-undergoing-assisted-reproductive-technologies
#7
Ardeshir Bahmanimehr, Shahryar Zeighami, Bahia Namavar Jahromi, Zahra Anvar, Mohammad Ebrahim Parsanezhad, Maryam Davari, Somayeh Montazeri
Background: Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with abnormal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive technologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are commonly required for infertility management of patients carrying YCDs. The aim of this study was to estimate the frequency of YCDs, to find the most frequent variant in infertile men candidate for ART and to compare YCD distribution with a control fertile group...
July 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29692680/microdissection-testicular-sperm-extraction-in-five-japanese-patients-with-non-mosaic-klinefelter-s-syndrome
#8
Makoto Chihara, Kanna Ogi, Tatsuya Ishiguro, Kunihiko Yoshida, Chikako Godo, Koichi Takakuwa, Takayuki Enomoto
Cases: Microdissection testicular sperm extraction (micro-TESE) was performed on five Japanese men with non-mosaic Klinefelter's syndrome (KS) and non-obstructive azoospermia in the authors' department. Here is reported the operative results and partner's clinical course for two cases where spermatozoa could be acquired. Also encountered was a man with non-mosaic KS with the partial deletion of azoospermia factor (AZF)b. Because this is rare, it is reported in detail in the context of the previous literature...
April 2018: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29687503/are-hormone-measurements-and-ultrasounds-really-predictors-of-sperm-retrieval-in-testicular-sperm-extraction-a-case-report-and-literature-review
#9
G Cito, M E Coccia, R Picone, A Cocci, L Criscuoli, S Dabizzi, G Nesi, S Serni, M Carini, A Natali
Azoospermia can be diagnosed in about 10%-15% of the infertile male population. To overcome the problem of failure to produce spermatozoa in the ejaculate in patients with nonobstructive azoospermia (NOA), testicular sperm extraction (TESE) may be performed to find the focal area of spermatogenesis. A 47-year-old man with NOA presented for treatment of secondary couple infertility. The patient underwent a first TESE 7 years earlier with cryopreservation, and an intracytoplasmic sperm injection-embryo transfer ended in a term pregnancy...
April 23, 2018: Andrologia
https://www.readbyqxmd.com/read/29684126/when-does-germ-cell-loss-and-fibrosis-occur-in-patients-with-klinefelter-syndrome
#10
D Van Saen, V Vloeberghs, I Gies, I Mateizel, K Sermon, Jean De Schepper, H Tournaye, E Goossens
STUDY QUESTION: When does germ cell loss and fibrosis occur in patients with Klinefelter syndrome (KS)? SUMMARY ANSWER: In KS, germ cell loss is not observed in testicular tissue from fetuses in the second semester of pregnancy but present at a prepubertal age when the testicular architecture is still normal, while fibrosis is highly present at an adolescent age. WHAT IS KNOWN ALREADY: Most KS patients are azoospermic at adult age because of a massive germ cell loss...
April 19, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29659092/diffusion-tensor-imaging-parameters-in-testes-with-nonobstructive-azoospermia
#11
Athina C Tsili, Alexandra Ntorkou, Anna Goussia, Loukas Astrakas, Eleni Panopoulou, Nikolaos Sofikitis, Maria I Argyropoulou
BACKGROUND: The development of noninvasive imaging parameters having the capacity to identify the population of men with nonobstructive azoospermia (NOA) where a successful sperm retrieval outcome is of great clinical significance. PURPOSE/HYPOTHESIS: To assess differences of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) in NOA testes with impaired spermatogenesis and the possible association with the presence of spermatozoa after testicular sperm extraction (TESE)...
April 16, 2018: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/29602610/successful-microdissection-testicular-sperm-extraction-for-men-with-non-obstructive-azoospermia
#12
REVIEW
Rauni Klami, Harri Mankonen, Antti Perheentupa
Non-obstructive azoospermia (NOA) is the most severe form of male infertility, defined by lack of spermatozoa in the ejaculate caused by impaired spermatogenesis. The chance of biological fatherhood of these men has been improved since the introduction of microdissection testicular sperm extraction (MD-TESE) combined with intracytoplasmic sperm injection. A thorough patient evaluation preoperatively is essential to recognize any underlying conditions, and to assist in patient counseling on the sperm recovery rate and pregnancy results...
March 27, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29568019/beneficial-effects-of-supervised-toothbrushing-on-caries-incidence-in-children-and-adolescents-are-questioned
#13
Ruth M Santamaria, Charles Splieth
Data sourcesThe Cochrane Central Register of Controlled Trials, Medline via PubMed, Web of Science, Embase, LILACS and BBO. Sources of grey literature included Open Grey, EThOS and Banco de Teses CAPES. Two international registers of ongoing trials were also searched (Current Controlled Trials and ClinicalTrials.gov). There were no language or date restrictions.Study selectionNo information is given in the manuscript or the published protocol on how the study selection was carried out, although the authors state that they followed PRISMA guidelines...
March 23, 2018: Evidence-based Dentistry
https://www.readbyqxmd.com/read/29512178/klinefelter-syndrome-and-fertility-impact-of-x-chromosomal-inheritance-on-spermatogenesis
#14
S Franik, D Smeets, G van de Zande, I Gomes, K D'Hauwers, D D M Braat, K Fleischer, L Ramos
With the use of testicular sperm extraction (TESE), spermatozoa can be retrieved in about 30%-50% of men with Klinefelter syndrome (KS). The reason for the absence or presence of spermatozoa in half of the men with KS remains unknown. Therefore, the search for an objective marker for a positive prediction in finding spermatozoa is of significant clinical value to avoid unnecessary testicular biopsies in males with (mostly) low testicular volume and impaired testosterone. The objective of this study was to determine whether paternal or maternal inheritance of the additional X-chromosome can predict the absence or presence of spermatogenesis in men with KS...
March 7, 2018: Andrologia
https://www.readbyqxmd.com/read/29511156/outcome-of-oocyte-vitrification-combined-with-microdissection-testicular-sperm-extraction-and-aspiration-for-assisted-reproduction-in-men
#15
Geng An, Zihao Zou, Ryan Flannigan, Jianqiao Liu, Hongzi Du, Xin Fu, Feixiang Guo, Wen Zhang
BACKGROUND As a safety and efficacy protocol, oocyte vitrification has been widely used in IVF treatment. The aim of this study was to evaluate the outcome of ICSI-ET utilizing vitrified oocytes with sperm obtained from non-obstructive azoospermia (NOA) patients via micro-TESE. MATERIAL AND METHODS A total of 150 NOA patients underwent micro-TESE. Ten patients were unable to ejaculate and refused to accept TESA at the time of oocyte retrieval; later, these patients underwent TESA. A total of 174 obstructive azoospermia (OA) patients underwent TESA...
March 7, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29460056/testicular-pirna-profile-comparison-between-successful-and-unsuccessful-micro-tese-retrieval-in-noa-patients
#16
Congcong Cao, Yujiao Wen, Xiaoli Wang, Na Fang, Shuiqiao Yuan, Xunbin Huang
PURPOSE: PIWI-interacting RNA (piRNA) is a sub-group of small RNAs about 30 nucleotides length which specifically expressed in mammalian germ cells. Although piRNAs play pivotal roles in spermatogenesis regulation, little is known in the testicular tissues of infertile men. To explore whether piRNA profile could serve as a biomarker for male infertility diagnosis in a clinic, in this study, we systematically investigated the expression profile of piRNAs in testicular tissues from the patients with non-obstructive azoospermia (NOA) between successful and unsuccessful sperm retrieval before micro-dissection testicular sperm extraction (micro-TESE)...
February 19, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29446828/novel-insights-on-testicular-volume-and-testosterone-replacement-therapy-in-klinefelter-patients-undergoing-testicular-sperm-extraction-a-retrospective-clinical-study
#17
Andrea Garolla, Riccardo Selice, Massimo Menegazzo, Umberto Valente, Filiberto Zattoni, Massimo Iafrate, Tommaso Prayer-Galetti, Marina P Gardiman, Alberto Ferlin, Andrea Di Nisio, Carlo Foresta
STUDY QUESTION: To investigate whether sperm recovery is related to clinical features, hormone parameters and testosterone replacement therapy (TRT) in patients with Klinefelter syndrome (KS). SUMMARY ANSWER: This study provides three interesting insights: (i) the probability to retrieve sperm is not related to testicular volume; (ii) TRT does not affect sperm retrieval rate (SRR); and (iii) reduced levels of LH and FSH represent a negative predictor of sperm retrieval in patients with TRT...
May 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29416867/onco-testicular-sperm-extraction-onco-tese-from-a-single-testis-with-metachronous-bilateral-testicular-cancer-a-case-report
#18
Itsuto Hamano, Shingo Hatakeyama, Rika Nakamura, Rie Fukuhara, Daisuke Noro, Hiroko Seino, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Yoshihito Yokoyama, Chikara Ohyama
Background: Although oncologic testicular sperm extraction (onco-TESE) has been increasingly practiced, the evidence of onco-TESE performed in patients with testicular cancer is insufficient. Furthermore, in bilateral testicular cancer, accounting for 0.5%-1% of testicular cancers, onco-TESE is more challenging and has been insufficiently reported. Case presentation: Here we report the case of a 25-year-old man who underwent onco-TESE from his residual single testis with a nonseminomatous germ cell tumor that occurred 5 years after orchiectomy of the contralateral testis...
2018: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/29413225/microdissection-testicular-sperm-extraction-micro-tese-predictive-value-of-preoperative-hormonal-levels-and-pathology-in-non-obstructive-azoospermia
#19
Alper Eken, Feray Gulec
The aim of this study was to evaluate the predictive value of preoperative hormonal levels and pathology, as well as the outcome of microsurgical testicular sperm extraction in patients with non-obstructive azoospermia (NOA), presenting to our clinic for treatment of infertility. The records of 145 men with NOA who underwent microdissection testicular sperm extraction (micro-TESE) between March 2013 and November 2016 were studied. The patient's age, testicular volume, hormonal profile for follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (TT), and testicular pathology were recorded...
February 2018: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29382506/klinefelter-syndrome-more-than-hypogonadism
#20
REVIEW
George A Kanakis, Eberhard Nieschlag
Klinefelter syndrome (KS) is the most frequent chromosome disorder in males (1:650 newborn males), defined by 47,XXY karyotype. The classical phenotype is that of a tall male with relatively long legs, small, firm testes and gynecomastia. Azoospermia and infertility are almost inevitably present, but may be overcome by TESE and ICSI. Nevertheless, a broad spectrum of phenotypes has been described and more than 70% of the actually existing KS men may remain undiagnosed throughout their lifespan. Accordingly, hypogonadism is usually not evident until early adulthood and progresses with ageing...
January 31, 2018: Metabolism: Clinical and Experimental
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