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Vitamin d binding protein

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https://www.readbyqxmd.com/read/29775620/a-monoclonal-antibody-sandwich-elisa-for-vitamin-d-binding-protein-vdbp-is-unaffected-by-gc-globulin-phenotype-peptides-and-actin-and-demonstrates-reduced-levels-in-sepsis-and-non-sepsis-intensive-care-patients
#1
Katrina Hong, Christopher M Florkowski, Matthew P Doogue, Peter A Elder, John G Lewis
The measurement of vitamin D-binding protein (VDBP) by immunoassay has been confounded by variable antibody recognition of the Gc1s, Gc1F and Gc2 phenotypes. This has led to spurious conclusions regarding vitamin D status in different ethnic groups. In order to overcome these problems there is a requirement for VDBP antibodies that are unaffected by phenotype status. Here we report the generation and testing of three monoclonal antibodies to VDBP which recognise linear epitopes and are unaffected by vast molar excesses of synthetic peptides spanning these phenotypic domains...
May 15, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29742726/igf-and-igfbp-as-an-index-for-discrimination-between-vitamin-d-supplementation-responders-and-nonresponders-in-overweight-saudi-subjects
#2
Nasser M Al-Daghri, Sobhy M Yakout, Kaiser Wani, Malak Nawaz Khan Khattak, Spiro D Garbis, George P Chrousos, Omar S Al-Attas, Majed S Alokail
Vitamin D deficiency is common in the Kingdom of Saudi Arabia (KSA). Therefore, it is significant to recognize which biochemical markers modulate serum 25 hydroxyvitamin D (25(OH)D) in response to vitamin D supplementation in such a population. Our aim was to study the correlation of insulin-like growth factor (IGF) and insulin growth factor binding protein (IGFBP) with serum 25(OH)D in response to vitamin D supplementation in a Saudi population. A total of 199 (89 males/110 females) vitamin D deficient subjects (25(OH)D level <50 nmol/L), aged 40...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29721809/gushukang-exerts-osteopreserve-effects-by-regulating-vitamin-d-and-calcium-metabolism-in-ovariectomized-mice
#3
Xiao-Li Li, Liang Wang, Xiao-Lei Bi, Bing-Bing Chen, Yan Zhang
Calcium homeostasis plays vital roles in the management of bone health. Traditional herbal formula Gushukang (GSK) was clinically applied to treat primary osteoporosis. This study aimed to explore the osteoprotective effects of GSK and its roles in maintaining calcium homeostasis in ovariectomized (OVX) mice. The OVX mice were orally treated with low (0.38 g/kg), middle (0.76 g/kg) and high (1.52 g/kg) dose of GSK for 8 weeks. GSK treatment dramatically increased serum calcium level and decreased urinary calcium excretion as well as enhanced calcium content in bone of OVX mice...
May 2, 2018: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29710028/vitamin-d-deficiency-increases-lipogenesis-and-reduces-beta-oxidation-in-the-liver-of-diet-induced-obese-mice
#4
Celina Carvalho Borges, Andreza Fernandes Salles, Isabele Bringhenti, Carlos Alberto Mandarim-DE-Lacerda, Marcia Barbosa Aguila
The study was conducted to understand better the mechanisms involved in liver changes when there is a combination of diet-induced obesity (DIO) and vitamin D deficiency (VDD). After 8 wk of feeding a control diet (C group) or a high-fat diet (HF), both with vitamin D, and counterpart groups without vitamin D (VitD- groups), we found in plasma: higher alanine aminotransferase, and aspartate aminotransferase in the VitD- groups, and more elevated total cholesterol in the HF group. Compared to their counterparts, HF and HF/VitD- showed hyperinsulinemia and higher hepatic triglycerides and steatosis...
2018: Journal of Nutritional Science and Vitaminology
https://www.readbyqxmd.com/read/29681950/vitamin-d-promotes-msc-osteogenic-differentiation-stimulating-cell-adhesion-and-%C3%AE-v-%C3%AE-3-expression
#5
Francesca Posa, Adriana Di Benedetto, Elisabetta A Cavalcanti-Adam, Graziana Colaianni, Chiara Porro, Teresa Trotta, Giacomina Brunetti, Lorenzo Lo Muzio, Maria Grano, Giorgio Mori
Vitamin D (Vit D) by means of its biological active form, 1 α ,25-dihydroxyvitamin D3 (1,25(OH)2 D3 ), has a protective effect on the skeleton by acting on calcium homeostasis and bone formation. Furthermore, Vit D has a direct effect on mesenchymal stem cells (MSCs) in stimulating their osteogenic differentiation. In this work, we present for the first time the effect of 1,25(OH)2 D3 on MSC adhesion. Considering that cell adhesion to the substrate is fundamental for cell commitment and differentiation, we focused on the expression of α V β 3 integrin, which has a key role in the commitment of MSCs to the osteoblastic lineage...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29678393/positive-correlations-between-free-vitamin-d-and-bone-variables-in-a-group-of-young-lebanese-women
#6
Abir Alwan, Maroun Rizkallah, Ghassan Maalouf, Joseph Matta, Fabienne Frenn, Angélique Barakat, Falah Bachour, Amer Sebaaly, Abdel-Jalil Berro, Nathalie Al Rassy, Mirza Howayek, Hassane Zouhal, Rawad El Hage
Optimizing bone mass in adulthood is of great importance to prevent the occurrence of osteoporosis in later age. Vitamin D is an essential component of bone health. Low-serum vitamin D is associated with low bone mineral density (BMD), which is an important predictor of fracture risk. However, most cells, apart from renal tubular cells, are exposed to free rather than to total 25-hydroxyvitamin D. Whether free vitamin D would be a better marker than total vitamin D is still under debate. The aim of the present study was to explore the relationships between serum total vitamin D, vitamin D-binding protein (BP), free vitamin D, and bone parameters in a group of young Lebanese women...
March 26, 2018: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/29658431/in-silico-identification-and-screening-of-cyp24a1-inhibitors-3d-qsar-pharmacophore-mapping-and-molecular-dynamics-analysis
#7
Jayaraj John Marshal, Gopinath Krishnasamy, Jung-Kul Lee, Karthikeyan Muthusamy
Vitamin D is a key signalling molecule that plays a vital role in the regulation of calcium-phosphate homeostasis and bone remodelling. The circulating biologically active form of vitamin D is regulated by the catabolic mechanism of cytochrome P450 24-hydroxylase (CYP24A1) enzyme. The over-expression of CYP24A1 negatively regulates the vitamin D level, which is the causative agent of chronic kidney disease, osteoporosis, and several types of cancers. In this study, we found three potential lead molecules adverse to CYP24A1 through structure-based, atom-based pharmacophore and e-pharmacophore-based screening methods...
April 16, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29653103/sterol-regulatory-element-binding-protein-1-trans-activates-25-hydroxy-vitamin-d-3-24-hydroxylase-gene-expression-in-renal-proximal-tubular-cells
#8
Tomohiro Kagawa, Mina Kozai, Masashi Masuda, Nagakatsu Harada, Otoki Nakahashi, Mari Tajiri, Ryouhei Yoshikawa, Mari Nakao, Yuichiro Takei, Masayuki Iwano, Eiji Takeda, Yutaka Taketani, Hironori Yamamoto
The physiological activity of the steroid derived hormone vitamin D is regulated by several enzymatic steps. Both 25-hydroxy vitamin D3 1α-hydroxylase (CYP27B1) and 25-hydroxyvitamin D3 24-hydroxylase (CYP24A1) modulate blood levels of 1,25-dihydroxyvitamin D3 , an activated form of vitamin D. We previously demonstrated that CYP27B1 expression was trans-activated by sterol regulatory element binding protein 1 (SREBP1), although whether SREBP1 also regulates CYP24A1 transcription was unclear. Here we investigated the ability of SREBP1 to affect CYP24A1 transcription...
April 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29628342/meg3-activated-by-vitamin-d-inhibits-colorectal-cancer-cells-proliferation-and-migration-via-regulating-clusterin
#9
Yan Zhu, Peizhan Chen, Yisha Gao, Na Ta, Yunshuo Zhang, Jialin Cai, Yong Zhao, Shupeng Liu, Jianming Zheng
The long non-coding RNA maternally expressed gene 3 (MEG3) is frequently dysregulated in human cancers; however, its roles in colorectal cancer (CRC) development are largely unknown. Here, we reported that MEG3 was down-regulated in CRC tissues and CRC patients with lower MEG3 showed poorer overall survival and disease-free survival than those with higher MEG3 level. MEG3 over-expression represses CRC cells proliferation and migration in vivo and in vitro, while MEG3 knockdown leads to the enhanced proliferation and metastasis of CRC cells...
March 31, 2018: EBioMedicine
https://www.readbyqxmd.com/read/29613921/markers-of-environmental-enteric-dysfunction-are-associated-with-neurodevelopmental-outcomes-in-tanzanian-children
#10
Analee J Etheredge, Karim Manji, Mark Kellogg, Hao Tran, Enju Liu, Christine M McDonald, Rodrick Kisenge, Said Aboud, Wafaie Fawzi, David Bellinger, Andrew T Gewirtz, Christopher P Duggan
BACKGROUND: Chronic exposure to enteropathogens may result in environmental enteric dysfunction (EED), a subclinical condition associated with poor child growth. Growth faltering is strongly associated with poor neurodevelopment, and occurs during sensitive periods of postnatal brain development. We investigated the role of novel EED biomarkers, systemic inflammation, and micronutrient status on neurodevelopment in Tanzanian children. METHODS: Non-stunted subjects with 6-week and 6-month blood samples and neurodevelopmental measures (n = 107) were included in this study...
April 2, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29602840/vitamin-d-obesity-and-leptin-in-relation-to-bladder-cancer-incidence-and-survival-prospective-protocol-study
#11
Randi Elin Gislefoss, Jo Steinson Stenehjem, Helga Helseth Hektoen, Bettina Kulle Andreassen, Hilde Langseth, Karol Axcrona, Elisabete Weiderpass, Alison Mondul, Trude Eid Robsahm
INTRODUCTION: Bladder cancer (BC) (including renal pelvis, ureter and urethra) is one of the most common urogenital cancers and the fourth most frequent cancer in men in the USA. In Norway, the incidence of BC has increased over the last decades. The age-standardised incidence rates per 100 000 for 2011-2015 were 53.7 in men and 16.5 in women. Compared to the 5-year period 2006-2010, the percentage increase in incidence was 6.1% in men and 12.3% in women. The recurrence rate of BC is over 50%, the highest recurrence rate of any malignancy...
March 30, 2018: BMJ Open
https://www.readbyqxmd.com/read/29599679/seasonal-variation-of-asthma-control-lung-function-tests-and-allergic-inflammation-in-relation-to-vitamin-d-levels-a-prospective-annual-study
#12
Sehra Birgül Batmaz, Tuğba Arıkoğlu, Lülüfer Tamer, Gülçin Eskandari, Semanur Kuyucu
Introduction: There are scarce data about the role of vitamin D (vitD) in asthma control related to seasons and other confounders. Aim: To investigate the seasonal relationship between vitD levels and asthma control, lung function tests (LFTs) and cytokines during a 1-year period, among 7-17-year-old asthmatic children. Material and methods: Thirty patients with asthma with house dust mite monosensitization were evaluated 3 monthly about the previous month's health and vitD related lifestyle factors and asthma control test (ACT), spirometry and bronchial provocation test for a year...
February 2018: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/29597233/vitamin-d-assays
#13
Daniel D Bikle
The number of requests for vitamin D metabolite measurements has increased dramatically over the past decade leading commercial laboratories to develop rapid high throughput assays. The measurement of 25-hydroxyvitamin D (25[OH]D) and to a lesser extent 1,25-dihydroxyvitamin D (1,25[OH]2D) dominates these requests, but requests for multiple metabolite measurements in the same sample are also increasing. The most commonly used methods include immunoassays and liquid chromatography/mass spectrometry (LC-MS). Each method has its advantages and disadvantages, but with improvements in technology, especially in LC-MS, this method is gaining ascendance due to its greater precision and flexibility...
2018: Frontiers of Hormone Research
https://www.readbyqxmd.com/read/29594051/vitamin-d-in-graves-disease-levels-correlation-with-laboratory-and-clinical-parameters-and-genetics
#14
Tereza Planck, Bushra Shahida, Johan Malm, Jonas Manjer
Objective: The aim was to compare the vitamin D levels in patients with Graves disease (GD) with the general population and to correlate the vitamin D levels with laboratory and clinical parameters in GD. Moreover, we examined the genetic variation in genes involved in the vitamin D metabolism and their association with GD. Methods: The levels of vitamin D were compared in 292 patients with newly diagnosed GD and 2,305 controls. Single nucleotide polymorphisms (SNPs) in the vitamin D receptor (VDR), vitamin D binding protein (DBP), and 1-α-hydroxylase ( CYP27B1 ) were examined for association with GD and/or Graves ophthalmopathy (GO) in 708 patients and 1,178 controls...
January 2018: European Thyroid Journal
https://www.readbyqxmd.com/read/29593729/genomic-effects-of-the-vitamin-d-receptor-potentially-the-link-between-vitamin-d-immune-cells-and-multiple-sclerosis
#15
REVIEW
Ming Lu, Bruce V Taylor, Heinrich Körner
Vitamin D has a plethora of functions that are important for the maintenance of general health and in particular, the functional integrity of the immune system, such as promoting an anti-inflammatory cytokine profile and reducing the Treg/Th17 ratio. Multiple sclerosis (MS) is a chronic, inflammatory, and neurodegenerative central nervous system (CNS) disorder of probable autoimmune origin. MS is characterized by recurring or progressive demyelination and degeneration of the CNS due in part to a misguided immune response to as yet undefined (CNS) antigens, potentially including myelin basic protein and proteolipid protein...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29567112/reference-intervals-for-measured-and-calculated-free-25-hydroxyvitamin-d-in-normal-pregnancy
#16
Oleg Tsuprykov, Claudia Buse, Roman Skoblo, Afrozul Haq, Berthold Hocher
The determination of free 25-hydroxyvitamin D (25(OH)D) as compared to the analysis of total 25-hydroxyvitamin D might reflect better the vitamin D status during pregnancy, since vitamin D-binding protein (DBP) concentrations increase throughout pregnancy and the vast majority of 25(OH)D is tightly bound to DBP thus strongly influencing total 25(OH)D. The concentration of the biologically active free 25(OH)D - on the other hand - is much less dependent on the DBP concentrations. The study was conducted in May-June 2016 in 368 Caucasian pregnant healthy women - residents of Northeastern Germany...
March 19, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29560610/investigating-the-effect-of-testosterone-by-itself-and-in-combination-with-letrozole-on-1-25-dihydroxy-vitamin-d-and-fgf23-in-male-rats
#17
F Saki, S R Kasaee, F Sadeghian, F Koohpeyma, G H R Omrani
BACKGROUND: Testosterone deficiency might be associated with vitamin D levels in hypogonadal men, but it is not clear whether testosterone can affect vitamin D and fibroblast growth factor-23 (FGF23), either directly or indirectly via aromatization to estradiol. We aimed to investigate the role of testosterone on vitamin D metabolism and serum FGF23 in male rats. METHODS: A total of 48 male rats were divided into 4 equal groups: sham; O, orchiectomy; O + T, orchiectomized rats treated with testosterone; and O + T + L, orchiectomized rats treated with combination of testosterone and letrozole...
March 20, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29556070/vitamin-d-alleviates-lead-induced-renal-and-testicular-injuries-by-immunomodulatory-and-antioxidant-mechanisms-in-rats
#18
Mohammad A BaSalamah, Abdelghany Hassan Abdelghany, Mohamed El-Boshy, Jawwad Ahmad, Shakir Idris, Bassem Refaat
This study measured the effects of vitamin D (VD) supplementation on the underlying molecular pathways involved in renal and testicular damage induced by lead (Pb) toxicity. Thirty two adult male Wistar rats were divided equally into four groups that were treated individually or simultaneously, except the negative control, for four weeks with lead acetate in drinking water (1,000 mg/L) and/or intramuscular VD (1,000 IU/kg; 3 days/week). Pb toxicity markedly reduced serum VD and Ca2+ , induced substantial renal and testicular injuries with concomitant significant alterations in the expression of VD metabolising enzymes, its receptor and binding protein, and the calcium sensing receptor...
March 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29554047/vitamin-d-binding-protein-dbp-deficiency-in-mice-decreases-systemic-and-select-tissue-levels-of-inflammatory-cytokines-in-a-murine-model-of-acute-muscle-injury
#19
Richard R Kew, Tahmineh Tabrizian, James A Vosswinkel, James E Davis, Randeep S Jawa
BACKGROUND: Severe acute muscle injury results in massive cell damage, causing the release of actin into extracellular fluids where it complexes with the vitamin D binding protein (DBP). We hypothesized that a systemic Vitamin D deficiency would result in a less pro-inflammatory phenotype. METHODS: C57BL/6 wild-type and DBP deficient (DBP-/-) mice received intramuscular injections of either 50% glycerol or phosphate-buffered saline into thigh muscles. Muscle injury was assessed by histology...
March 16, 2018: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29549963/identification-of-hla-a2-restricted-immunogenic-peptides-derived-from-vitamin-d-binding-protein
#20
Min Zhang, Guangmin Lu, Fanqing Meng, Shufa Li, Xunhua Li, Xiaoyun Gong
T-cell-mediated destruction of pancreatic β cells leads to Type 1 diabetes (TID). Vitamin D-Binding Protein (VDBP) has been identified as an autoantigen and T cell reactivity against VDBP increases in the development of T1D. Autoreactive cytotoxic T lymphocytes (CTLs) recognize β-cell-derived peptides in the context of major histocompatibility complex class I molecules. However, little is known about the VDBP-derived immunogenic peptides that are presented in the context of human HLA molecules. Here, we predicted and identified VDBP derived immunogenic peptides that were presented in association with human HLA-A2 molecule...
March 5, 2018: Cellular Immunology
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