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Vitamin d binding protein

Ean-Jeong Seo, Nicolas Fischer, Thomas Efferth
The translationally controlled tumor protein (TCTP) is a highly conserved protein that is regulated due to a high number of extracellular stimuli. TCTP has an important role for cell cycle and normal development. On the other side, tumor reversion and malignant transformation have been associated with TCTP. TCTP has been found among the 12 genes that are differentially expressed during mouse oocyte maturation, and an overexpression of this gene was reported in a wide variety of different cancer types. Its antiapoptotic effect is indicated by the interaction with several proapoptotic proteins of the Bcl-2 family and the p53 tumor suppressor protein...
2017: Results and Problems in Cell Differentiation
Sherri L Surman, Bart G Jones, David L Woodland, Julia L Hurwitz
Previous research has evaluated antibody responses toward an influenza virus vaccine in the context of deficiencies for vitamins A and D (VAD+VDD). Results showed that antibodies and antibody-forming cells in the respiratory tract were reduced in VAD+VDD mice. However, effectors were recovered when oral supplements of vitamins A + D were delivered at the time of vaccination. Here we address the question of how vaccine-induced CD8(+) T cell responses are affected by deficiencies for vitamins A + D. VAD+VDD and control mice were vaccinated with an intranasal, cold-adapted influenza virus A/Puerto Rico/8/34 vaccine, with or without oral supplements of vitamins A + D...
November 13, 2017: Viral Immunology
William E Winter, Sherri D Flax, Neil S Harris
Primary hemostasis begins with endothelial injury. VWF, produced by endothelial cells, binds to platelets and links them to subendothelial collagen. Platelet-derived ADP and thromboxane activate non-adhered platelets via their GPIIb/IIIa receptors, allowing these platelets to participate in platelet aggregation. Secondary hemostasis is initiated with the binding of factor VII to extravascular tissue factor (TF). Factors II, VII, IX and X are vitamin K-dependent factors. The role of vitamin K is to assist in the addition of gamma carboxylate groups to glutamic acids in the "GLA" domains of these factors...
November 8, 2017: Laboratory Medicine
Inmaculada Moreno-Santos, Daniel Castellano-Castillo, María Fernanda Lara, Jose Carlos Fernandez-Garcia, Francisco Jose Tinahones, Manuel Macias-Gonzalez
Adipose tissue has traditionally only been considered as an energy storage organ. Nevertheless, the importance of this tissue in systemic physiology and, especially, in systemic inflammation has been highlighted in recent years. Adipose tissue expresses proteins related to vitamin D (VD) metabolism, and it has been proposed that it can act as a VD storage tissue. The active form of VD, 1,25-dihydroxyvitamin D3 (1,25(OH)₂D₃), is able to modify adipocyte and adipose tissue physiology via the VD receptor (VDR), decreasing the expression of pro-inflammatory cytokines in adipose tissue...
November 7, 2017: International Journal of Molecular Sciences
Jeremy D Cook, Sze Yin Ng, Marcia Lloyd, Shannon Eddington, Hui Sun, Jeremy Nathans, Dean Bok, Roxana A Radu, Gabriel H Travis
Peropsin is a nonvisual opsin in both vertebrate and invertebrate species. In mammals, peropsin is present in the apical microvilli of retinal pigment epithelial (RPE) cells. These structures interdigitate with the outer segments (OS) of rod and cone photoreceptor cells. RPE cells play critical roles in the maintenance of photoreceptors, including the recycling of visual chromophore for the opsin visual pigments. Here, we sought to identify the function of peropsin in the mouse eye. To this end, we generated mice with a null mutation in the peropsin gene (Rrh)...
November 6, 2017: Journal of Biological Chemistry
M Abboud, M S Rybchyn, Y J Ning, T C Brennan-Speranza, C M Girgis, J E Gunton, D R Fraser, R S Mason
The major circulating metabolite of vitamin D3, 25-hydroxycholecalciferol [25(OH)D], has a remarkably long half-life in blood for a (seco)steroid. Data from our studies and others are consistent with the hypothesis that there is a role for skeletal muscle in the maintenance of vitamin D status. Muscle cells internalise vitamin D-binding protein (DBP) from the circulation by means of a megalin/cubilin plasma membrane transport mechanism. The internalised DBP molecules then bind to actin and thus provide an intracellular array of high affinity binding sites for its specific ligand, 25(OH)D...
October 26, 2017: Journal of Steroid Biochemistry and Molecular Biology
María Julia Ferronato, Eliana Noelia Alonso, Débora Gisele Salomón, María Eugenia Fermento, Norberto Ariel Gandini, Mario Alfredo Quevedo, Evangelina Mascaró, Cristian Vitale, Yagamare Fall, María Marta Facchinetti, Alejandro Carlos Curino
Glioblastoma multiforme (GBM) is the worst and most common brain tumor, characterized by high proliferation and invasion rates. The current standard treatment is mainly based on chemoradiotherapy and this approach has slightly improved patient survival. Thus, novel strategies aimed at prolonging the survival and ensuring a better quality of life are necessary. In the present work, we investigated the antitumoral effect of the novel analogue of calcitriol EM1 on GBM cells employing in vitro, in silico, and in vivo assays...
November 1, 2017: Journal of Steroid Biochemistry and Molecular Biology
Hiroki Mano, Shinichi Ikushiro, Nozomi Saito, Atsushi Kittaka, Toshiyuki Sakaki
Split-luciferase techniques are widely used to detect protein-protein interaction and bioactive small molecules including some hormones and vitamins. Previously, we successfully expressed chimeric proteins of luciferase and the ligand binding domain (LBD) of the vitamin D receptor (VDR), LucC-LBD-LucN in COS-7 cells. The LucC-LBD-LucN biosensor was named split-luciferase vitamin D biosensor (SLDB). This biosensor can detect and discriminate between VDR agonists and antagonists in mammalian cells. In this study, we established an in vitro screening system for VDR ligands using the SLDB proteins expressed in Escherichia coli (E...
October 31, 2017: Journal of Steroid Biochemistry and Molecular Biology
Xi Wen, Lorenzo Franchi, Feng Chen, Yan Gu
Background: Detection of pubertal growth peak is vital in orthodontic treatment timing and planning. Gingival crevicular fluid (GCF) contains abundant proteins from different sources and is an ideal source of biomarkers. The aim of this research is to detect candidate GCF biomarkers of pubertal growth by tandem mass tags (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to help diagnosis and treatment planning of functional treatment. Methods: Forty subjects were recruited and were divided into pubertal and post-pubertal groups according to cervical vertebral maturation method...
October 28, 2017: European Journal of Orthodontics
Kai Zhao, Xiaoqian Luan, Yuntao Liu, Xinjie Tu, Huijun Chen, Huiping Shen, Huihua Qiu, Zhuoying Zhu, Jincai He
BACKGROUND: The association between low 25-hydroxyvitamin D [25(OH)D] and sleep disorder has been reported. We investigated whether serum concentrations of 25(OH)D are altered in chronic insomnia patients. The relationship between serum concentrations of 25(OH)D and the treatment outcome in patients at 2months was also investigated. METHODS: In total, 181 chronic insomnia patients were consecutively recruited. All patients received pharmacotherapy for the treatment of chronic insomnia...
October 26, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
Daniel D Bikle, Sofie Malmstroem, Janice Schwartz
The free hormone hypothesis postulates that only the nonbound fraction (the free fraction) of hormones that otherwise circulate in blood bound to their carrier proteins is able to enter cells and exert their biologic effects. For the vitamin D metabolites less than 1% (0.4% for 1,25(OH)2D and 0.03% for 25(OH)D) is free, with more than 99% bound to the vitamin D binding protein (DBP) and albumin (approximately 85% and 15%, respectively). Assays to measure the free vitamin D metabolite levels have been developed, and initial studies indicated their value in subjects with altered DBP levels...
December 2017: Endocrinology and Metabolism Clinics of North America
S Vemulapati, E Rey, D O'Dell, S Mehta, D Erickson
Vitamin D is necessary for the healthy growth and development of bone and muscle. Vitamin D deficiency, which is present in 42% of the US population, is often undiagnosed as symptoms may not manifest for several years and long-term deficiency has been linked to osteoporosis, diabetes and cancer. Currently the majority of vitamin D testing is performed in large-scale commercial laboratories which have high operational costs and long times-to-result. Development of a low-cost point-of-need assay could be transformative to deficiency analysis in limited-resource settings...
October 26, 2017: Scientific Reports
Simon P J Albracht, Johannes van Pelt
With the substrate DNP-α-GalNAc (2,4-dinitrophenyl-N-acetyl-α-d-galactosaminide) three α-N-acetylgalactosaminidase-like activities could be distinguished in serum, in addition to the classical lysosomal enzyme (Naga, EC, pH optimum at 4). Two activities had optima in the pH 5 to 6 region and one peaked around pH 8. Like the Naga activity at pH 4, the activity at pH 8 was detectable under standard assay conditions. However, the two activities in the pH 5 to 6 range were not readily apparent in such assays...
December 2017: BBA Clinical
Pilar Peris, Xavier Filella, Ana Monegal, Nuria Guañabens, Laura Foj, María Bonet, Dolors Boquet, Enrique Casado, Dacia Cerdá, Alba Erra, Carmen Gómez-Vaquero, Silvia Martínez, Nuria Montalá, Concepción Pittarch, Eduardo Kanterewicz, Miquel Sala, Xavier Suris, Josep L Carrasco
At present, data comparing the quantification methods for measurement of free vitamin D (direct assay [direct 25-OHDF] and estimated by calculation [calculated 25-OHDF]), are scarce. The aim of this study was to analyse the concordance between these two methods of 25-OHDF analysis (direct vs. calculated). METHODS: Serum values of total 25-OHD (25-OHDT), vitamin D binding protein (DBP) (by R&D Systems ELISA), calculated 25-OHDF (by DBP, albumin and 25-OHDT) and direct 25-OHDF (by DIAsource ELISA) were analysed in 173 healthy women (aged 35-45years)...
October 19, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
Huiying Lu, Rui-Di Xie, Ritian Lin, Cuicui Zhang, Xiao-Jun Xiao, Lin-Jing Li, Zhi-Qiang Liu, Li-Tao Yang, Bai-Sui Feng, Zhan-Ju Liu, Ping-Chang Yang
Eosinophils (Eo) play a critical role in immunity and immune inflammation. The maintenance of Eo homeostasis is not fully understood yet. Vitamin D (VitD) is involved in the regulation of a large number of biochemical reactions. This study tests a hypothesis that VitD receptor (VDR) contributes to the homeostasis of Eos. In this study, EoL-1 cells (an Eo cell line) were cultured in the presence or absence of calcitriol. The Eo-mediators, including major basic protein (MBP), Eo peroxidase (EPX), Eo cationic protein (ECP) and Eo-derived neurotoxin (EDN), were assessed in the culture supernatant and in EoL-1 cells...
October 12, 2017: Cellular Immunology
Jason Franasiak, Sue Shapses, Wei Sun, Richard Scott, Xiangbing Wang
BACKGROUND: The importance of vitamin D in general health as well as in human reproductive success has been an area of focus. A better understanding of vitamin D metabolism, particularly vitamin D binding protein, is important when elucidating this relationship. METHODS: This case control trial seeks to characterize vitamin D metabolism in infertile patients undergoing natural cycle IVF as compared to normally cycling premenopausal women with proven fertility matched for age and body mass index (BMI)...
2017: Fertility Research and Practice
Zhenchang Zhang, Qi Yan, Jia Guo, Xueping Wang, Wei Yuan, Lei Wang, Lixia Chen, Gang Su, Manxia Wang
Methylene Tetrahydrofolate Reductase (MTHFR) catalyzes the conversion of methylene tetrahydrofolate to methylte trahydrofolate. The 677th nucleotide of the MTHFR gene is often regarded as a risk factor of cardiovascular disease. Previous studies demonstrated an elevated risk of ischemic stroke with the MTHFR677TT genotype. In this study, we employed a plasma proteomics method to investigate the connection between the polymorphism of the target nucleotide and stroke. In total, 28 protein spots were differentially expressed between the two groups, and of which, 25 protein spots were up-regulated and 3 were down-regulated...
October 17, 2017: Scientific Reports
Damián Escribano, Hüseyin Cihan, Silvia Martínez-Subiela, Pinar Levent, Meric Kocaturk, Nilüfer Aytug, Jose Joaquin Cerón, Asta Tvarijonaviciute, Zeki Yilmaz
The aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to Group 1 and 10 to Group 2) and 10 healthy dogs. Two-dimensional electrophoresis (2DE) of pooled serum for each of the group of dogs were run in triplicate...
October 16, 2017: Microbial Pathogenesis
Anne Marie Z Jukic, Andrew N Hoofnagle, Pamela L Lutsey
Vitamin D is a fat-soluble vitamin that is synthesized in the skin with exposure to sunlight, or ingested from dietary supplements or food. There has been a dramatic increase in research on vitamin D, linking it with health outcomes as varied as reproductive function, infection, cardiovascular disease, and cancer. Vitamin D has generated much excitement, in part because it is an ideal intervention: low levels may be common and can be remedied with widely-available supplements. Determination of vitamin D status is complex, and has advanced dramatically in the past five years...
August 17, 2017: American Journal of Epidemiology
Lisa E Kilpatrick, Karen W Phinney
Vitamin-D-binding protein (VDBP), a transporter of 25-hydroxyvitamin D metabolites, has three common isoforms. The relationship of the isoforms and their glycosylation state with various diseases has been under recent examination. In this work, liquid chromatography coupled to isotope dilution mass spectrometry was evaluated for quantification of VDBP, the three common isoforms, and total glycosylation. Protocols using guanidine, urea, RapiGest, trifluoroethanol, or tris buffer were also evaluated for optimal tryptic digestion...
October 16, 2017: Journal of Proteome Research
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