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https://www.readbyqxmd.com/read/29656441/a-review-on-current-status-of-antiviral-sirna
#1
REVIEW
Abid Qureshi, Vaqar Gani Tantray, Altaf Rehman Kirmani, Abdul Ghani Ahangar
Viral diseases like influenza, AIDS, hepatitis, and Ebola cause severe epidemics worldwide. Along with their resistant strains, new pathogenic viruses continue to be discovered so creating an ongoing need for new antiviral treatments. RNA interference is a cellular gene-silencing phenomenon in which sequence-specific degradation of target mRNA is achieved by means of complementary short interfering RNA (siRNA) molecules. Short interfering RNA technology affords a potential tractable strategy to combat viral pathogenesis because siRNAs are specific, easy to design, and can be directed against multiple strains of a virus by targeting their conserved gene regions...
April 15, 2018: Reviews in Medical Virology
https://www.readbyqxmd.com/read/29656222/prolactin-induced-protein-pip-is-a-potential-biomarker-for-early-stage-and-malignant-breast-cancer
#2
Anju Gangadharan, Themba Nyirenda, Kishan Patel, Nydia Jaimes-Delgadillo, Dominique Coletta, Takemi Tanaka, Ayal C Walland, Zena Jameel, Srinivasa Vedantam, Sittinon Tang, Ciaran Mannion, Grace Y Lee, Andre Goy, Andrew Pecora, K Stephen Suh
OBJECTIVES: Breast cancer (BC) is the second leading cause of cancer-related mortality in women. Bioinformatic analysis and expression screening showed that Prolactin Induced Protein (PIP) was differentially expressed in BC. The objective of this investigation was to characterize the expression pattern of PIP, an aspartyl proteinase, in malignant and non-malignant breast tissues. MATERIALS AND METHODS: Real time quantitative PCR was employed to analyze PIP and androgen receptor (AR) mRNA levels in BC cell lines and 190 normal tissues and tumor samples...
April 12, 2018: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/29656205/a-nano-bio-interfacial-protein-corona-on-silica-nanoparticle
#3
Hongyan Zhang, Jiaxi Peng, Xin Li, Shengju Liu, Zhengyan Hu, Guiju Xu, Ren'an Wu
Nano-bio interaction takes the crucial role in bio-application of nanoparticles. The systematic mapping of interfacial proteins remains the big challenge as low level of proteins within interface regions and lack of appropriate technology. Here, a facile proteomic strategy was developed to characterize the interfacial protein corona (noted as IPC) that has strong interactions with silica nanoparticle, via the combination of the vigorous elution with high concentration sodium dodecyl sulfate (SDS) and the pre-isolation of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...
April 8, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29656123/selenium-supplementation-prevents-metabolic-and-transcriptomic-responses-to-cadmium-in-mouse-lung
#4
Xin Hu, Joshua D Chandler, Jolyn Fernandes, Michael L Orr, Li Hao, Karan Uppal, David C Neujahr, Dean P Jones, Young-Mi Go
BACKGROUND: The protective effect of selenium (Se) on cadmium (Cd) toxicity is well documented, but underlying mechanisms are unclear. METHODS: Male mice fed standard diet were given Cd (CdCl2 , 18 μmol/L) in drinking water with or without Se (Na2 SeO4, 20 μmol/L) for 16 weeks. Lungs were analyzed for Cd concentration, transcriptomics and metabolomics. Data were analyzed with biostatistics, bioinformatics, pathway enrichment analysis, and combined transcriptome-metabolome-wide association study...
April 12, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29653362/the-long-noncoding-rna-zfas1-facilitates-bladder-cancer-tumorigenesis-by-sponging-mir-329
#5
Jin-Shan Wang, Qing-Hong Liu, Xiao-Hua Cheng, Wen-Yuan Zhang, Yong-Chao Jin
The incidence and mortality rate of bladder cancer have dramatically expanded, so it's urgent to discover new biomarker and therapeutic target for bladder cancer. Recently, lncRNA has been identified as oncogene or tumor suppressor to regulate the tumorigenesis. LncRNA ZFAS1 has been confirmed as oncogene in various tumors. However, the expression, function, and underlying mechanism of ZFAS1 in bladder carcinogenesis have yet to be totally clarified. In the current study, our data demonstrated that ZFAS1 expression was significantly upregulated in bladder cancer tissues and cell lines...
April 10, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29653352/online-molecular-image-repository-and-analysis-system-a-multicenter-collaborative-open-source-infrastructure-for-molecular-imaging-research-and-application
#6
Mahabubur Rahman, Hiroshi Watabe
Molecular imaging serves as an important tool for researchers and clinicians to visualize and investigate complex biochemical phenomena using specialized instruments; these instruments are either used individually or in combination with targeted imaging agents to obtain images related to specific diseases with high sensitivity, specificity, and signal-to-noise ratios. However, molecular imaging, which is a multidisciplinary research field, faces several challenges, including the integration of imaging informatics with bioinformatics and medical informatics, requirement of reliable and robust image analysis algorithms, effective quality control of imaging facilities, and those related to individualized disease mapping, data sharing, software architecture, and knowledge management...
April 4, 2018: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29653233/three-novel-variants-p-glu178lys-p-val245met-p-ser250phe-of-the-phenylalanine-hydroxylase-pah-gene-impair-protein-expression-and-function-in-vitro
#7
Yanan Zong, Ning Liu, Shanshan Ma, Ying Bai, Fangxia Guan, Xiangdong Kong
Phenylketonuria (PKU) is the most common inherited metabolic disease, an autosomal recessive disorder affecting >10,000 newborns each year globally. It can be caused by over 1000 different naturally occurring mutations in the phenylalanine hydroxylase (PAH) gene. We analyzed three novel naturally occurring PAH gene variants: p.Glu178Lys (c.532G>A), p.Val245Met (c.733G>A) and p.Ser250Phe (c.749C>T). The mutant effect on the PAH enzyme structure and function was predicted by bioinformatics software...
April 10, 2018: Gene
https://www.readbyqxmd.com/read/29653195/genome-wide-integration-study-of-circulating-mirnas-and-peripheral-whole-blood-mrnas-of-male-acute-ischemic-stroke-patients
#8
Yang Xue, Pengqi Yin, Guozhong Li, Di Zhong
Several circulating microRNAs (miRNAs) have been proved to serve as stable biomarkers in blood for acute ischemic stroke (AIS). However, the functions of these biomarkers remain elusive. By conducting the integration analysis of circulating miRNAs and peripheral whole blood mRNAs using bioinformatics methods, we explored the biological role of these circulating markers in peripheral whole blood at the genome-wide level. Stroke-related circulating miRNA profile data (GSE86291) and peripheral whole blood mRNA expression data (GSE16561) were collected from the Gene Expression Omnibus (GEO) datasets...
April 10, 2018: Neuroscience
https://www.readbyqxmd.com/read/29652992/-phenotypic-and-mutational-analysis-of-a-pedigree-affected-with-hereditary-coagulation-factor-%C3%A2-deficiency
#9
Mengcha Tian, Hong Xia, Zhishan Zhang, Yanhui Jin, Kankan Su, Mingshan Wang
OBJECTIVE: To explore the molecular pathogenesis for a pedigree affected with coagulation factor Ⅴ (FⅤ) deficiency. METHODS: Prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), coagulation factor Ⅱ activity (FⅡ: C), FⅤ activity (FⅤ: C), coagulation factor Ⅶ activity (FⅦ: C), and coagulation factor Ⅹ activity (FⅩ: C) were determined with a STAGO automatic coagulometer. FⅤ antigen (FⅤ: Ag) was detected with enzyme linked immunosorbent assay (ELISA)...
April 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/29652933/rhogapp190-a-potential-player-in-tbph-mediated-neurodegeneration-in-drosophila
#10
Simona Langellotti, Giulia Romano, Fabian Feiguin, Francisco Ernesto Baralle, Maurizio Romano
TDP-43 is an ubiquitous and highly conserved ribonucleoprotein involved in several cellular processes including pre-mRNA splicing, transcription, mRNA stability and transport. Notwithstanding the evidence of TDP-43 involvement in the pathogenesis of different neurodegenerative disorders (i.e. ALS and FTLD), the underlying mechanisms are still unclear. Given the high degree of functional similarity between the human and fly orthologs of TDP-43, Drosophila melanogaster is a simple and useful model to study the pathophysiological role of this protein in vivo...
2018: PloS One
https://www.readbyqxmd.com/read/29651297/niclosamide-blocks-rice-leaf-blight-by-inhibiting-biofilm-formation-of-xanthomonas-oryzae
#11
Sunil Kumar Sahu, Ping Zheng, Nan Yao
Rice ( Oryza sativa ) is the leading source of nutrition for more than half of the world's population, and by far it is the most important commercial food crop. But, its growth and production are significantly hampered by the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) which causes leaf blight disease. Earlier studies have reported the antibacterial ability of FDA-approved niclosamide drug against Xoo. However, the underlying mechanism by which niclosamide blocks the growth of Xoo remained elusive...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29651275/structural-and-functional-motifs-in-influenza-virus-rnas
#12
REVIEW
Damien Ferhadian, Maud Contrant, Anne Printz-Schweigert, Redmond P Smyth, Jean-Christophe Paillart, Roland Marquet
Influenza A viruses (IAV) are responsible for recurrent influenza epidemics and occasional devastating pandemics in humans and animals. They belong to the Orthomyxoviridae family and their genome consists of eight (-) sense viral RNA (vRNA) segments of different lengths coding for at least 11 viral proteins. A heterotrimeric polymerase complex is bound to the promoter consisting of the 13 5'-terminal and 12 3'-terminal nucleotides of each vRNA, while internal parts of the vRNAs are associated with multiple copies of the viral nucleoprotein (NP), thus forming ribonucleoproteins (vRNP)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29651107/circular-rna-circ-4099-is-induced-by-tnf-%C3%AE-and-regulates-ecm-synthesis-by-blocking-mir-616-5p-inhibition-of-sox9-in-intervertebral-disc-degeneration
#13
Hua Wang, Peiheng He, Hehai Pan, Jun Long, Jianru Wang, Zemin Li, Hui Liu, Weiying Jiang, Zhaomin Zheng
Circular RNAs (circRNAs) play important roles in the initiation and development of different diseases. Here, we detected their role in intervertebral disc (IVD) degeneration. An Arraystar human circular RNA microarray assay was used to detect circRNAs in normal and degenerated human IVD nucleus pulposus (NP) tissues. The role of circ-4099 in IVDD and its mechanism were evaluated by qRT-PCR and gain-of-function/loss-of-function studies. Interaction networks for competing endogenous RNAs (ceRNAs), miRNAs, and miRNA target gene were detected by bioinformatics analysis, RNA immunoprecipitation and luciferase assay...
April 13, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29650642/setd2-regulates-quiescence-and-differentiation-of-adult-hematopoietic-stem-cells-by-restricting-rna-polymerase-ii-elongation
#14
Yile Zhou, Xiaomei Yan, Xiaomin Feng, Jiachen Bu, Yunzhu Dong, Peipei Lin, Yoshihiro Hayashi, Rui Huang, Andre Olsson, Paul R Andreassen, H Leighton Grimes, Qian-Fei Wang, Tao Cheng, Zhijian Xiao, Jie Jin, Gang Huang
SET domain containing 2 (Setd2), encoding a histone methyltransferase, is associated with many hematopoietic diseases when mutated. By generating a novel exon 6 conditional knockout mouse model, we described an essential role of Setd2 in maintaining the adult hematopoietic stem cells. Loss of Setd2 results in leukopenia, anemia, and increased platelet accompanied with hypocellularity, erythroid dysplasia, and mild fibrosis in bone marrow. Setd2 knockout mice show significantly decreased hematopoietic stem and progenitor cells except for erythroid progenitors...
April 12, 2018: Haematologica
https://www.readbyqxmd.com/read/29650461/towards-designing-a-synthetic-antituberculosis-vaccine-the-rv3587c-peptide-inhibits-mycobacterial-entry-to-host-cells
#15
Mary Lilian Carabali-Isajar, Marisol Ocampo, Deisy Carolina Rodriguez, Magnolia Vanegas, Hernando Curtidor, Manuel Alfonso Patarroyo, Manuel Elkin Patarroyo
Mycobacterium tuberculosis is considered one of the most successful pathogens in the history of mankind, having caused 1.7 million deaths in 2016. The amount of resistant and extensively resistant strains has increased; BCG has been the only vaccine to be produced in more than 100 years though it is still unable to prevent the disease's most disseminated form in adults; pulmonary tuberculosis. The search is thus still on-going for candidate antigens for an antituberculosis vaccine. This paper reports the use of a logical and rational methodology for finding such antigens, this time as peptides derived from the Rv3587c membrane protein...
April 4, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29650443/investigation-of-mir-136-5p-key-target-genes-and-pathways-in-lung-squamous-cell-cancer-based-on-tcga-database-and-bioinformatics-analysis
#16
Zu-Cheng Xie, Tian-Tian Li, Bin-Liang Gan, Xiang Gao, Li Gao, Gang Chen, Xiao-Hua Hu
BACKGROUND: Lung squamous cell cancer (LUSC) is a common but challenging malignancy. It is important to illuminate the molecular mechanism of LUSC. Thus, we aim to explore the molecular mechanism of miR-136-5p in relation to LUSC. METHODS: We used the Cancer Genome Atlas (TCGA) database to investigate the expression of miR-136-5p in relation to LUSC. Then, we identified the possible miR-136-5p target genes through intersection of the predicted miR-136-5p target genes and LUSC upregulated genes from TCGA...
April 5, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29650251/exploring-the-molecular-mechanisms-of-traditional-chinese-medicine-components-using-gene-expression-signatures-and-connectivity-map
#17
Minjae Yoo, Jimin Shin, Hyunmin Kim, Jihye Kim, Jaewoo Kang, Aik Choon Tan
BACKGROUND AND OBJECTIVE: Traditional Chinese Medicine (TCM) has been practiced over thousands of years in China and other Asian countries for treating various symptoms and diseases. However, the underlying molecular mechanisms of TCM are poorly understood, partly due to the "multi-component, multi-target" nature of TCM. To uncover the molecular mechanisms of TCM, we perform comprehensive gene expression analysis using connectivity map. METHODS: We interrogated gene expression signatures obtained 102 TCM components using the next generation Connectivity Map (CMap) resource...
April 4, 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29650230/production-of-spliced-peptides-by-the-proteasome
#18
Nathalie Vigneron, Vincent Stroobant, Violette Ferrari, Joanna Abi Habib, Benoit J Van den Eynde
CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments of proteins that were liberated after the hydrolysis of the peptide bonds located at their extremities. However, several examples of peptides containing two fragments originally distant in the protein sequence challenged this concept and demonstrated that proteasome could also splice peptides together by creating a new peptide bond between two distant fragments...
April 9, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29649778/in-silico-design-of-small-peptides-antagonist-against-leptin-receptor-for-the-treatment-of-obesity-and-its-associated-immune-mediated-diseases
#19
Manne Munikumar, Vagolu Siva Krishna, Varikasuvu Seshadri Reddy, Bolla Rajeswari, Dharmarajan Sriram, Mendu Vishnuvardhana Rao
Excess adiposity in obese inhibits negatively impacts immune function and host defence. Obesity is characterized by a state of low-grade, chronic inflammation in addition to disturbed levels of circulating nutrients and metabolic hormones. The impact of metabolic abnormalities on obesity-related co-morbidities has undergone intense scrutiny over the past decades. Thus, treatment of obesity and its associated immune-mediated diseases is challenging due to impaired function of leptin system. These disorders are managed through antibiotics and by cytokines replacement...
April 4, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29649577/bioinformatics-characterization-of-a-cathepsin-b-transcript-from-the-giant-river-prawn-macrobrachium-rosenbergii-homology-modeling-and-expression-analysis-after-aeromonas-hydrophila-infection
#20
Saowalak Onming, Wilawan Thongda, Chao Li, Orathai Sawatdichaikul, Nichanun McMillan, Sirawut Klinbunga, Eric Peatman, Supawadee Poompuang
Cathepsin B is a lysosomal proteolytic enzyme that has been suggested to play a role in pathological processes of immune system. In this study, the full-length cDNA sequence of cathepsin B transcript in the giant river prawn Macrobrachium rosenbergii (MrCTSB) was obtained from 454 pyrosequencing of cDNAs from hepatopancreas and muscle. It was 1158 bp in length, containing an open reading frame (ORF) of 987 bp corresponding to 328 amino acids. The predicted molecular mass and pI of MrCTSB protein was 36...
April 9, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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