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https://www.readbyqxmd.com/read/28641403/a-molasses-habitat-derived-fungus-aspergillus-tubingensis-xg21-with-high-%C3%AE-fructofuranosidase-activity-and-its-potential-use-for-fructooligosaccharides-production
#1
Yijia Xie, Huanxia Zhou, Caixia Liu, Jing Zhang, Ning Li, Zhanli Zhao, Guoyong Sun, Yaohua Zhong
The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-enriched biotope would help improve FOS productivity and reduce the process cost. Here, three fungi isolated from a unique sugarcane molasses habitat were found to possess FFase activity and one of them, XG21, exhibited a high capacity to synthesize FOS...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28641400/cytosolic-delivery-of-sirna-by-ultra-high-affinity-dsrna-binding-proteins
#2
Nicole J Yang, Monique J Kauke, Fangdi Sun, Lucy F Yang, Katie F Maass, Michael W Traxlmayr, Yao Yu, Yingda Xu, Robert S Langer, Daniel G Anderson, K Dane Wittrup
Protein-based methods of siRNA delivery are capable of uniquely specific targeting, but are limited by technical challenges such as low potency or poor biophysical properties. Here, we engineered a series of ultra-high affinity siRNA binders based on the viral protein p19 and developed them into siRNA carriers targeted to the epidermal growth factor receptor (EGFR). Combined in trans with a previously described endosome-disrupting agent composed of the pore-forming protein Perfringolysin O (PFO), potent silencing was achieved in vitro with no detectable cytotoxicity...
June 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28641251/robust-face-recognition-with-kernelized-locality-sensitive-group-sparsity-representation
#3
Shoubiao Tan, Xi Sun, Wentao Chan, Lei Qu, Ling Shao
In this paper, a novel joint sparse representation method is proposed for robust face recognition. We embed both group sparsity and kernelized locality-sensitive constraints into the framework of sparse representation. The group sparsity constraint is designed to utilize the grouped structure information in the training data. The local similarity between test and training data is measured in the kernel space instead of the Euclidian space. As a result, the embedded nonlinear information can be effectively captured, leading to a more discriminative representation...
June 15, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28641227/presence-distribution-and-risk-assessment-of-polycyclic-aromatic-hydrocarbons-in-rice-wheat-continuous-cropping-soils-close-to-five-industrial-parks-of-suzhou-china
#4
Yong Li, Ling Long, Jing Ge, Li-Xuan Yang, Jin-Jin Cheng, Ling-Xiang Sun, Changying Lu, Xiang-Yang Yu
Polycyclic aromatic hydrocarbons (PAHs) accumulated in agricultural soils are likely to threaten human health and ecosystem though the food chain, therefore, it is worth to pay more attention to soil contamination by PAHs. In this study, the presence, distribution and risk assessment of 16 priority PAHs in rice-wheat continuous cropping soils close to industrial parks of Suzhou were firstly investigated. The concentrations of the total PAHs ranged from 125.99 ng/g to 796.65 ng/g with an average of 352.94 ng/g...
June 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28641174/new-insights-into-the-oleate-flotation-response-of-feldspar-particles-of-different-sizes-anisotropic-adsorption-model
#5
Longhua Xu, Jia Tian, Houqin Wu, Wei Deng, Yaohui Yang, Wei Sun, Zhiyong Gao, Yuehua Hu
The anisotropic adsorption of sodium oleate (NaOL) on feldspar surfaces was investigated to elucidate the different flotation properties of feldspar particles of four different size ranges. Microflotation experiments showed that the feldspar flotation recovery of particles with sizes spanning different ranges decreased in the order 0-19>19-38>45-75>38-45μm. Zeta potential and FTIR measurements showed that NaOL was chemically adsorbed on the Al sites of the feldspar surface. The anisotropic surface energies and broken bond densities estimated by density functional theory calculations showed that, although feldspar mostly exposed (010) and (001) surfaces, only the (001) surfaces contained the Al sites needed for NaOL adsorption...
June 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28641162/triterpenoids-and-sterols-from-the-grains-of-echinochloa-utilis-ohwi-yabuno-and-their-cytotoxic-activity
#6
Thi Trang Nguyen, Duc Hung Nguyen, Bing Tian Zhao, Duc Dat Le, Byung Sun Min, Young Ho Kim, Mi Hee Woo
Two new tetracyclic triterpenoids, echinochlorins C (1) and D (2), and sawamilletin (3) with new spectroscopic data were isolated from Echinochloa utilis Ohwi & Yabuno grains, along with one known triterpenoid (4) and eight sterols (5-12). Their structures were elucidated by spectroscopic data analyses (IR, UV, MS, and NMR). These compounds were tested in vitro cytotoxic activities against the human tumor-cell lines (HeLa, HL-60, and MCF-7). Compounds 6 and 8 displayed potential cytotoxic activity against HeLa, with IC50 values of 3...
June 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28641124/neural-repair-by-nt3-chitosan-via-enhancement-of-endogenous-neurogenesis-after-adult-focal-aspiration-brain-injury
#7
Peng Hao, Hongmei Duan, Fei Hao, Lan Chen, Min Sun, Kevin S Fan, Yi Eve Sun, David Williams, Zhaoyang Yang, Xiaoguang Li
The latent regenerative potential of endogenous neural stem/progenitor cells (NSCs) in the adult mammalian brain has been postulated as a likely source for neural repair. However, the inflammatory and inhibitory microenvironment after traumatic brain injury (TBI) prohibits NSCs from generating new functional neurons to restore brain function. Here we report a biodegradable material, chitosan, which, when loaded with neurotrophin-3 (NT3) and injected into the lesion site after TBI, effectively engaged endogenous NSCs to proliferate and migrate to the injury area...
April 26, 2017: Biomaterials
https://www.readbyqxmd.com/read/28641038/interaction-of-tinnitus-suppression-and-hearing-ability-after-cochlear-implantation
#8
Qian Wang, Jia-Nan Li, Guan-Xiong Lei, Dai-Shi Chen, Wei-Ze Wang, Ai-Ting Chen, Meng-Di Mong, Sun Li, Qing-Shan Jiao, Shi-Ming Yang
OBJECTIVES: To study the postoperative impact of cochlear implants (CIs) on tinnitus, as well as the impact of tinnitus on speech recognition with CI switched on. METHODS: Fifty-two postlingual deafened CI recipients (21 males and 31 females) were assessed using an established Tinnitus Characteristics Questionnaire and Tinnitus Handicap Inventory (THI) before and after cochlear implantation. The tinnitus loudness was investigated when CI was switched on and off in CI recipients with persistent tinnitus...
June 22, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28641005/electron-crystallography-reveals-atomic-structures-of-metal-organic-nanoplates-with-m12-%C3%AE-3-o-8-%C3%AE-3-oh-8-%C3%AE-2-oh-6-m-zr-hf-secondary-building-units
#9
Ruihan Dai, Fei Peng, Pengfei Ji, Kuangda Lu, Cheng Wang, Junliang Sun, Wenbin Lin
Nanoscale metal-organic frameworks (nMOFs) have shown tremendous potential in cancer therapy and biomedical imaging. However, their small dimensions present a significant challenge in structure determination by single-crystal X-ray crystallography. We report here the structural determination of nMOFs by rotation electron diffraction (RED). Two isostructural Zr- and Hf-based nMOFs with linear biphenyldicarboxylate (BPDC) or bipyridinedicarboxylate (BPYDC) linkers are stable under intense electron beams to allow the collection of high-quality RED data, which reveal a MOF structure with M12(μ3-O)8(μ3-OH)8(μ2-OH)6 (M = Zr, Hf) secondary building units (SBUs)...
June 22, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28641003/synthesis-and-anti-inflammatory-evaluation-of-2-aminobenzaldehydes-via-ir-iii-catalyzed-c-h-amidation-of-aldimines-with-acyl-azides
#10
Saegun Kim, Prashant Chakrasali, Hyo Sun Suh, Neeraj Kumar Mishra, Taeyoung Kim, Sang Hoon Han, Hyung Sik Kim, Byung-Moo Lee, Soo Bong Han, In Su Kim
The aldimine-directed C-H amidation of various arenes with N-acyl azides as amidation surrogates under cationic iridium(III) catalysis is described. This transformation efficiently provides a range of 2-aminobenzaldehydes derivatives with excellent site-selectivity and functional group compatibility. The resulting 2-aminobenzaldehyde framework provides facile access to a range of biologically interesting heterocycles. In addition, all synthetic compounds were screened for anti-inflammatory activity against interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) with lipopolysaccharide (LPS)-induced RAW264...
June 22, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28640985/the-interaction-between-progranulin-and-prosaposin-is-mediated-by-granulins-and-the-linker-region-between-saposin-b-and-c
#11
Xiaolai Zhou, Peter M Sullivan, Lirong Sun, Fenghua Hu
The frontotemporal lobar degeneration (FTLD) protein progranulin (PGRN) is essential for proper lysosomal function. PGRN localizes in the lysosomal compartment within the cell. Prosaposin (PSAP), the precursor of lysosomal saposin activators (saposin A, B, C, D), physically interacts with PGRN. Previously we have shown that PGRN and PSAP facilitate each other's lysosomal trafficking. Here we report that the interaction between PSAP and PGRN requires the linker region of saposin B and C (BC linker). PSAP protein with the BC linker mutated fails to interact with PGRN and target PGRN to lysosomes in the biosynthetic and endocytic pathways...
June 22, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28640905/an-efficient-method-for-transgenic-callus-induction-from-vitis-amurensis-petiole
#12
Tingting Zhao, Zemin Wang, Lingye Su, Xiaoming Sun, Jun Cheng, Langlang Zhang, Sospeter Karanja Karungo, Yuepeng Han, Shaohua Li, Haiping Xin
Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species...
2017: PloS One
https://www.readbyqxmd.com/read/28640877/identification-of-c-ebp%C3%AE-as-a-novel-target-of-the-hpv8-e6-protein-regulating-mir-203-in-human-keratinocytes
#13
Anna M Marthaler, Marta Podgorska, Pascal Feld, Alina Fingerle, Katrin Knerr-Rupp, Friedrich Grässer, Hans Smola, Klaus Roemer, Elke Ebert, Yoo-Jin Kim, Rainer M Bohle, Cornelia S L Müller, Jörg Reichrath, Thomas Vogt, Magdalena Malejczyk, Sławomir Majewski, Sigrun Smola
Patients suffering from Epidermodysplasia verruciformis (EV), a rare inherited skin disease, display a particular susceptibility to persistent infection with cutaneous genus beta-human papillomavirus (beta-HPV), such as HPV type 8. They have a high risk to develop non-melanoma skin cancer at sun-exposed sites. In various models evidence is emerging that cutaneous HPV E6 proteins disturb epidermal homeostasis and support carcinogenesis, however, the underlying mechanisms are not fully understood as yet. In this study we demonstrate that microRNA-203 (miR-203), a key regulator of epidermal proliferation and differentiation, is strongly down-regulated in HPV8-positive EV-lesions...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28640793/intraoperative-computed-tomography-ct-for-treating-giant-carotid-intracavernous-aneurysms
#14
Zhe Xue, Fuyu Wang, Zhenghui Sun, Hui Zhang, Chen Wu, Dongsheng Kong, Bainan Xu
BACKGROUND Giant carotid intracavernous aneurysm refers to those lesions larger than 2.5 cm and derived from a cavernous segment, accounting for about 30% of all intracranial tumors. Dynamic CT perfusion imaging (PCT) is a common method recently employed to evaluate cerebral perfusion. This study investigated the efficacy and clinical application of intraoperative CT in the surgery for giant symptomatic carotid intracavernous aneurysm. MATERIAL AND METHODS A retrospective analysis was performed on 23 cases with giant symptomatic carotid intracavernous aneurysm...
June 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28640589/ru-bpy-32-incorporated-luminescent-polymer-dots-double-enhanced-electrochemiluminescence-for-detection-of-single-nucleotide-polymorphism
#15
Yaqiang Feng, Feng Sun, Ningning Wang, Jianping Lei, Huangxian Ju
Encapsulating a large amount of electrochemiluminescence (ECL) active molecules into one nanoparticle for single target recognition can greatly improve the sensitivity of ECL assay. This work used luminescent conjugated polymer as a carrier to synthesize Ru(bpy)32+ doped polymer dots (RuPdots). The RuPdots with an average size of 20 nm and encapsulation of about 1354 Ru(bpy)32+ showed greatly enhanced ECL emission. Using tripropylamine as a co-reactant, the resonance energy transfer from the excited polymer dots to the encapsulated Ru(bpy)32+ was demonstrated, which led to 15...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28640578/the-insect-analogue-to-the-lotus-leaf-a-planthopper-wing-membrane-incorporating-a-low-adhesion-non-wetting-superhydrophobic-bactericidal-and-biocompatible-surface
#16
Gregory Shaun Watson, David W Green, Bronwen W Cribb, Christopher Leslie Brown, Christopher Merritt, Mark James Tobin, Jitraporn Vongsvivut, Ming-Xia Sun, Alping Liang, Jolanta Anna Watson
Nature has produced many intriguing and spectacular surfaces at the micro and nanoscales. These small surface decorations act for a singular or, in most cases, a range of functions. The minute landscape found on the lotus leaf is one such example, displaying anti-wetting behaviour and low adhesion with foreign particulate matter. Indeed the lotus leaf has often been considered the 'benchmark' for such properties. One could expect that there are animal counterparts of this self-drying and self-cleaning surface system...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28637337/matrix-completion-with-side-information-and-its-applications-in-predicting-the-antigenicity-of-influenza-viruses
#17
Li Huang, Xianhong Li, Pengfei Guo, Yuhua Yao, Bo Liao, Weiwei Zhang, Fayou Wang, Jiasheng Yang, Yulong Zhao, Hailiang Sun, Pingan He, Jialiang Yang
Motivation: Low-rank matrix completion has been demonstrated to be powerful in predicting antigenic distances among influenza viruses and vaccines from partially revealed hemagglutination inhibition (HI) table. Meanwhile, influenza hemagglutinin (HA) protein sequences are also effective in inferring antigenic distances. Thus, it is natural to integrate HA protein sequence information into low-rank matrix completion model to help infer influenza antigenicity, which is critical to influenza vaccine development...
June 16, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28637318/experience-dependent-regulation-of-cajal-retzius-cell-networks-in-the-developing-and-adult-mouse-hippocampus
#18
Max Anstötz, Sun Kyong Lee, Tamra I Neblett, Gabriele M Rune, Gianmaria Maccaferri
In contrast to their near-disappearance in the adult neocortex, Cajal-Retzius cells have been suggested to persist longer in the hippocampus. A distinctive feature of the mature hippocampus, not maintained by other cortical areas, is its ability to sustain adult neurogenesis. Here, we have investigated whether environmental manipulations affecting hippocampal postnatal neurogenesis have a parallel impact on Cajal-Retzius cells. We used multiple mouse reporter lines to unequivocally identify Cajal-Retzius cells and quantify their densities during postnatal development...
June 16, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28637158/a-reused-method-for-molasses-processed-wastewater-effect-on-silage-quality-and-anaerobic-digestion-performance-of-pennisetum-purpereum
#19
Lianhua Li, Zhenhong Yuan, Yongming Sun, Xiaoying Kong, Pengyu Dong, Jia Zhang
The silage quality and anaerobic digestion performance of Pennisetum purpereum with molasses-processed wastewater addition were assessed. The silage samples with molasses-processed wastewater addition obtained higher lactic acid concentration of 15.18-23.38mg/g FM, lower pH value of 3.96-4.45 and lower NH3-N content of 0.47-0.64mg/g FM. No obvious difference was observed in the dominant phyla and genus, but the relative abundance of Lactobacillus reached up to 77.39%, increased by 84% compared to the silage samples without molasses-processed wastewater addition...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28637105/cytomegalovirus-gastric-ulcer-complicated-with-pyloric-obstruction-in-a-patient-with-ulcerative-colitis
#20
Sung Hwan Kang, Kee Myung Lee, Sung Jae Shin, Sun Kyo Lim, Jae Chul Hwang, Jin Hong Kim
In patients with inflammatory bowel disease (IBD), cytomegalovirus (CMV) infections could aggravate the course of IBD but it is difficult to distinguish CMV infection from IBD exacerbation endoscopically. Usually, CMV tends to localize to the colon and other organic involvements were reported very rare in the IBD patients. Herein, we report a case that CMV gastric ulcer complicated with pyloric obstruction in a patient with ulcerative colitis during ganciclovir therapy, which was resolved by surgical gastrojejunostomy with review of literature...
June 25, 2017: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
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