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Molecular cell biology

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https://www.readbyqxmd.com/read/28346821/structure-activity-relationships-on-cynnamoyl-derivatives-as-inhibitors-of-p300-histone-acetyltransferase
#1
Valentina Noemi Madia, Rosaria Benedetti, Maria Letizia Barreca, Liza Ngo, Luca Pescatori, Antonella Messore, Giovanni Pupo, Francesco Saccoliti, Sergio Valente, Antonello Mai, Luigi Scipione, Yujun George Zheng, Cristina Tintori, Maurizio Botta, Violetta Cecchetti, Lucia Altucci, Roberto Di Santo, Roberta Costi
Human p300 is a polyhedric transcriptional coactivator, playing a crucial role by acetylating histones on specific lysine residues. A great deal of evidences shows that p300 is involved in several diseases as leukemia, tumors and viral infection. Its involvement in pleiotropic biological roles and connections to diseases provide the rationale as to how its modulation could represent an amenable drug target. Several p300 inhibitors (HATi) have been described so far, but all suffer from low potency, lack of specificity or low cell-permeability, highlighting the need to find more effective inhibitors...
March 27, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28345878/multispectral-optical-tweezers-for-biochemical-fingerprinting-of-cd9-positive-exosome-subpopulations
#2
Randy P Carney, Sidhartha Hazari, Macalistair Colquhoun, Di Tran, Billanna Hwang, Michael S Mulligan, James D Bryers, Eugenia Girda, Gary S Leiserowitz, Zachary James Smith, Kit S Lam
Extracellular vesicles (EVs), including exosomes, are circulating nanoscale particles heavily implicated in cell signaling, and can be isolated in vast numbers from human biofluids. Study of their molecular profiling and materials properties is currently underway for purposes of describing a variety of biological functions and diseases. However, the large, and as yet unquantified, variety of EV subpopulations differing in composition, size, and likely function, necessitates characterization schemes capable of measuring single vesicles...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28345638/lectin-like-oxidized-low-density-lipoprotein-receptor-1-facilitates-metastasis-of-gastric-cancer-through-driving-epithelial-mesenchymal-transition-and-pi3k-akt-gsk3%C3%AE-activation
#3
Can Li, Jie Zhang, Hao Wu, Lili Li, Caiting Yang, Shushu Song, Peike Peng, Miaomiao Shao, Mingming Zhang, Junjie Zhao, Ran Zhao, Weicheng Wu, Yuanyuan Ruan, Lan Wang, Jianxin Gu
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a pattern recognition receptor that plays a critical role in vascular diseases and host immune response. Recently, our research discovered that LOX-1 could facilitate the uptake of dying cells and cross-presentation of cellular antigen via binding with heat shock proteins, which have a close relationship with gastric neoplasia. Therefore, we speculated that LOX-1 may serve as an oncogene in gastric cancer (GC) development and progression. In this study, through immunohistochemistry staining assay and cancer-related databases, we found that LOX-1 expression was up-regulated in GC tissues and correlated with a poor prognosis in GC patients...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345453/tumor-suppressor-mir-29c-regulates-radioresistance-in-lung-cancer-cells
#4
Elena Arechaga-Ocampo, Cesar Lopez-Camarillo, Nicolas Villegas-Sepulveda, Claudia H Gonzalez-De la Rosa, Isidro X Perez-Añorve, Reynalda Roldan-Perez, Ali Flores-Perez, Omar Peña-Curiel, Oscar Angeles-Zaragoza, Rosalva Rangel Corona, Juan A Gonzalez-Barrios, Raul Bonilla-Moreno, Oscar Del Moral-Hernandez, Luis A Herrera, Alejandro Garcia-Carranca
Radiotherapy is an important treatment option for non-small cell lung carcinoma patients. Despite the appropriate use of radiotherapy, radioresistance is a biological behavior of cancer cells that limits the efficacy of this treatment. Deregulation of microRNAs contributes to the molecular mechanism underlying resistance to radiotherapy in cancer cells. Although the functional roles of microRNAs have been well described in lung cancer, their functional roles in radioresistance are largely unclear. In this study, we established a non-small cell lung carcinoma Calu-1 radioresistant cell line by continuous exposure to therapeutic doses of ionizing radiation as a model to investigate radioresistance-associated microRNAs...
March 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28345336/proteomic-identification-of-eef1a1-as-a-molecular-target-of-curcumol-for-suppressing-metastasis-of-mda-mb-231-cells
#5
Hongyi Qi, Ling Ning, Zanyang Yu, Guojun Dou, Li Li
Curcumol, a major volatile component in Rhizoma Curcumae, exhibited potent anti-metastatic effect on breast cancer cells. However, its molecular mechanism remains poorly understood. In this study, we employed a 2-DE based proteomics to investigate the cellular targets of curcumol in MDA-MB-231 cells, and identified 10 differentially expressed proteins. Moreover, Gene Ontology analysis revealed that those proteins mainly get involved in 9 types of cellular components, 7 different biological processes and 9 kinds of molecular functions, and 35 pathways (p < 0...
March 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28344971/how-to-train-a-cell-cutting-edge-molecular-tools
#6
REVIEW
Jakub Czapiński, Michał Kiełbus, Joanna Kałafut, Michał Kos, Andrzej Stepulak, Adolfo Rivero-Müller
In biological systems, the formation of molecular complexes is the currency for all cellular processes. Traditionally, functional experimentation was targeted to single molecular players in order to understand its effects in a cell or animal phenotype. In the last few years, we have been experiencing rapid progress in the development of ground-breaking molecular biology tools that affect the metabolic, structural, morphological, and (epi)genetic instructions of cells by chemical, optical (optogenetic) and mechanical inputs...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28344749/heavily-calcified-gastrointestinal-stromal-tumors-pathophysiology-and-implications-of-a-rare-clinicopathologic-entity
#7
Massimiliano Salati, Giulia Orsi, Luca Reggiani Bonetti, Fabrizio Di Benedetto, Giuseppe Longo, Stefano Cascinu
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract, and are characterized by a broad spectrum of clinical, histological and molecular features at presentation. Although focal and scattered calcifications are not uncommon within the primary tumor mass, heavy calcification within a GIST is rarely described in the literature and the clinical-biological meaning of this feature remains unclear. Cases with such an atypical presentation are challenging and may be associated with diagnostic pitfalls...
March 15, 2017: World Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/28344745/can-molecular-biomarkers-replace-a-clinical-risk-score-for-resectable-colorectal-liver-metastasis
#8
EDITORIAL
Torhild Veen, Kjetil Søreide
In resectable colorectal liver metastasis (CRLM) the role and use of molecular biomarkers is still controversial. Several biomarkers have been linked to clinical outcomes in CRLM, but none have so far become routine for clinical decision making. For several reasons, the clinical risk score appears to no longer hold the same predictive value. Some of the reasons include the ever expanding indications for liver resection, which now increasingly tend to involve extrahepatic disease, such as lung metastases (both resectable and non-resectable) and the shift in indication from "what is taken out" (e...
March 15, 2017: World Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/28344072/osteopontin-metallothionen-i-ii-interactions-in-experimental-autoimmunune-encephalomyelitis
#9
Hrvoje Jakovac, Tanja Grubić Kezele, Sandra Šućurović, Biserka Mulac-Jeričević, Biserka Radošević-Stašić
Osteopontin (OPN), an extracellular matrix (ECM) glyco-phosphoprotein, plays an important role in autoimmune-mediated demyelinating diseases, including multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). As an integrin and CD44 binding protein it participates in bidirectional communication between the ECM and target cells and affects transduction pathways that maintain neuronal and immune cells homeostasis. Its biological activity is also heavily influenced by microenvironment, which stimulates the cleavage of OPN and changes its functions...
March 23, 2017: Neuroscience
https://www.readbyqxmd.com/read/28344011/the-epidemiology-pathogenesis-transmission-diagnosis-and-management-of-multidrug-resistant-extensively-drug-resistant-and-incurable-tuberculosis
#10
REVIEW
Keertan Dheda, Tawanda Gumbo, Gary Maartens, Kelly E Dooley, Ruth McNerney, Megan Murray, Jennifer Furin, Edward A Nardell, Leslie London, Erica Lessem, Grant Theron, Paul van Helden, Stefan Niemann, Matthias Merker, David Dowdy, Annelies Van Rie, Gilman K H Siu, Jotam G Pasipanodya, Camilla Rodrigues, Taane G Clark, Frik A Sirgel, Aliasgar Esmail, Hsien-Ho Lin, Sachin R Atre, H Simon Schaaf, Kwok Chiu Chang, Christoph Lange, Payam Nahid, Zarir F Udwadia, C Robert Horsburgh, Gavin J Churchyard, Dick Menzies, Anneke C Hesseling, Eric Nuermberger, Helen McIlleron, Kevin P Fennelly, Eric Goemaere, Ernesto Jaramillo, Marcus Low, Carolina Morán Jara, Nesri Padayatchi, Robin M Warren
Global tuberculosis incidence has declined marginally over the past decade, and tuberculosis remains out of control in several parts of the world including Africa and Asia. Although tuberculosis control has been effective in some regions of the world, these gains are threatened by the increasing burden of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis. XDR tuberculosis has evolved in several tuberculosis-endemic countries to drug-incurable or programmatically incurable tuberculosis (totally drug-resistant tuberculosis)...
March 15, 2017: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/28343380/ratiometric-visualization-of-no-h2s-cross-talk-in-living-cells-and-tissues-using-a-nitroxyl-responsive-two-photon-fluorescence-probe
#11
Yibo Zhou, Xiufang Zhang, Sheng Yang, Yuan Li, Zhihe Qing, Jing Zheng, Jishan Li, Ronghua Yang
It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S), because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28342848/ultraviolet-a-induced-oxidation-in-cornea-characterization-of-the-early-oxidation-related-events
#12
Corinne Zinflou, Patrick J Rochette
Exposure to sunlight ultraviolet-A (UVA), the main component of solar UV reaching the eyes, is suspected to play an important part in the onset of ocular pathologies. UVA primary biological deleterious effects arise from the photo-induction of oxidative stress in cells. However, the molecular bases linking UVA-induced oxidation to UVA toxicity in eyes remain poorly understood, especially with regards to the cornea. To shed some light on this issue, we have investigated the susceptibility and response potential of the different corneal cellular layers (epithelium, stroma and endothelium) to UVA-induced oxidation...
March 22, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28342400/using-gene-expression-database-to-uncover-biology-functions-of-1-4-disubstituted-1-2-3-triazole-analogues-synthesized-via-a-copper-i-catalyzed-reaction
#13
Chun-Li Su, Chia-Ling Tseng, Chintakunta Ramesh, Hsiao-Sheng Liu, Chi-Ying F Huang, Ching-Fa Yao
We have synthesized bioactive 1,4-disubstituted 1,2,3-triazole analogues containing 2H-1,4-benzoxazin-3-(4H)-one derivatives via 1,3-dipolar cycloaddition in the presence of CuI. All the reactions proceeded smoothly and afforded its desired products in excellent yields. Among these analogues, 3y exhibited a better cytotoxic effect on human hepatocellular carcinoma (HCC) Hep 3B cells and displayed less cytotoxicity on normal human umbilical vein endothelial cells, compared with Sorafenib, a targeted therapy for advanced HCC...
March 18, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28342388/insights-into-the-molecular-and-biological-features-of-the-dutpase-related-gene-of-bovine-immunodeficiency-virus
#14
Yulia Podolny, Eytan Herzig, Amnon Hizi
This study was stimulated by our previous research of the dUTPase-related protein from bovine immunodeficiency virus (BIV) (Voronin et al., 2014). Despite the lack of detectable enzymatic BIV dUTPase activity (both of the recombinant protein and in virions), mutating the dUTPase gene was deleterious to viral production. However, cDNA synthesis and integration were apparently unaffected. Consequently, we have studied here two important issues. First, we showed that in cDNA produced by the dUTPase-mutated virions, the incidence of mutations was not higher than that found in wild-type BIV-infected cells...
March 22, 2017: Virology
https://www.readbyqxmd.com/read/28342366/discovery-of-new-dual-ppar%C3%AE-gpr40-agonists-with-robust-antidiabetic-activity-design-synthesis-and-in-combo-drug-evaluation
#15
Sergio Hidalgo-Figueroa, Gabriel Navarrete-Vázquez, Samuel Estrada-Soto, Diana Giles-Rivas, Francisco J Alarcón-Aguilar, Ismael León-Rivera, Abraham Giacoman-Martínez, Elizabeth Miranda Pérez, Julio C Almanza-Pérez
The design of compounds 1 and 2 was based on the similar scaffold of pharmacophoric groups for PPARγ and GPR40 agonists. In order to find new compounds with improved biological activity, the current manuscript describes a new dual PPARγ-GPR40 agonist. We synthesized two compounds, which were prepared following a multistep synthetic route, and the relative mRNA expression levels of PPARγ, GLUT4, and GPR40 were quantified in cell culture, as well as insulin secretion and [Ca(2+)] intracellular levels. Compound 1 showed a 7-times increase in the mRNA expression of PPARγ, which in turn enhanced the expression levels of GLUT4 respect to control and pioglitazone...
March 22, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28342334/oncogene-addiction-in-non-small-cell-lung-cancer-focus-on-ros1-inhibition
#16
REVIEW
Francesco Facchinetti, Giulio Rossi, Emilio Bria, Jean-Charles Soria, Benjamin Besse, Roberta Minari, Luc Friboulet, Marcello Tiseo
Detection of molecular aberrations driving the biology and the clinical behavior of advanced non-small cell lung cancer (NSCLC) allows the adoption of specific therapeutic strategies dramatically impacting disease courses. Among these, ROS1 rearrangements are present in 1-2% of lung adenocarcinomas. Thanks to similarities between ALK and ROS1 oncogenes, lessons inferred from ALK can be applied to ROS1-positive NSCLC; nevertheless, disparities exist between diseases mastered by these two fusion genes. In the absence of more common genetic alterations detected in NSCLC (e...
March 12, 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28341680/phar-a-negative-regulatory-protein-of-phap-modulates-the-colonization-of-burkholderia-gut-symbiont-in-the-midgut-of-the-host-insect-riptortus-pedestris
#17
Seong Han Jang, Ho Am Jang, Jun Beom Lee, Jong Uk Kim, Seung Ah Lee, Kyoung-Eun Park, Byung Hyun Kim, Yong Hun Jo, Bok Luel Lee
Five genes encoding PhaP family proteins and one phaR gene have been identified in the genome of Burkholderia RPE75. PhaP proteins function as the surface proteins of polyhydroxyalkanoate (PHA) granules, and PhaR protein acts as a negative regulator of PhaP biosynthesis. Recently, we characterized one phaP gene to understand the molecular cross-talk between Riptortus insects and Burkholderia gut symbionts. In this study, we constructed four other phaP gene-depleted mutants (ΔphaP1, ΔphaP2, ΔphaP3, ΔphaP4), one phaR gene-depleted mutant (ΔphaR) and a phaR-complemented mutant (ΔphaR/phaR)...
March 24, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28341599/nucleo-cytoplasmic-transport-of-estrogen-receptor-alpha-in-breast-cancer-cells
#18
REVIEW
Angeles C Tecalco-Cruz, Issis A Pérez-Alvarado, Josué O Ramírez-Jarquín, Leticia Rocha-Zavaleta
Approximately 70% cases of breast cancers exhibit high expression and activity levels of estrogen receptor alpha (ERα), a transcription regulator that induces the expression of genes associated with cellular proliferation and survival. These nuclear functions of the receptor are associated with the development of breast cancer. However, ERα localization is not static, but rather, dynamic with continuous shuttling between the nucleus and the cytoplasm. Interestingly, both the nuclear import and export of ERα are modulated by several stimuli that include estradiol, antiestrogens, and growth factors...
March 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28341226/lung-adenocarcinoma-and-squamous-cell-carcinoma-gene-expression-subtypes-demonstrate-significant-differences-in-tumor-immune-landscape
#19
Hawazin Faruki, Gregory M Mayhew, Jonathan S Serody, D Neil Hayes, Charles M Perou, Myla Lai-Goldman
INTRODUCTION: Molecular subtyping of lung Adenocarcinoma (AD) and lung Squamous Cell Carcinoma (SQ) reveal biologically diverse tumors that vary in their genomic and clinical attributes. METHODS: Using published immune cell signatures and several Lung AD and SQ gene expression datasets including The Cancer Genome Atlas (TCGA), immune response in relation to AD and SQ expression subtypes was examined. Expression of immune cell populations and other immune related genes including CD274 (PD-L1) was investigated in the tumor microenvironment relative to the expression subtypes of AD (Terminal Respiratory Unit (TRU), Proximal Proliferative (PP), and Proximal Inflammatory (PI)) and SQ subtypes (Primitive, Classical, Secretory, Basal)...
March 21, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28341078/isolation-molecular-characterization-and-in%C3%A2-vitro-differentiation-of-bovine-wharton-jelly-derived-multipotent-mesenchymal-cells
#20
Anna Lange-Consiglio, Claudia Perrini, Alessia Bertero, Paola Esposti, Fausto Cremonesi, Leila Vincenti
Extrafetal tissues are a noncontroversial and inexhaustible source of mesenchymal stem cells that can be harvested noninvasively at low cost. In the veterinary field, as in man, stem cells derived from extrafetal tissues express plasticity, reduced immunogenicity, and have high anti-inflammatory potential making them promising candidates for treatment of many diseases. Umbilical cord mesenchymal cells have been isolated and characterized in different species and have recently been investigated as potential candidates in regenerative medicine...
February 2017: Theriogenology
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