keyword
MENU ▼
Read by QxMD icon Read
search

Telomerase

keyword
https://www.readbyqxmd.com/read/29228452/establishment-and-analysis-of-the-prediction-model-for-cervical-squamous-cell-carcinoma
#1
Y-H Zhou, W-F Fan, J Deng, H-L Xi
OBJECTIVE: This study aimed to construct a prediction model for cervical squamous cell carcinoma and evaluate its accuracy in diagnosing cervical squamous cell carcinoma. PATIENTS AND METHODS: Fifty patients with initially histopathologically confirmed cervical squamous cell carcinoma and 150 patients with initially histopathologically confirmed cervical intraepithelial neoplasia (CIN) were enrolled. The high-risk human papillomavirus (HR-HPV) infection, human telomerase mRNA component (hTERC) gene and cell-myc (c-myc) gene amplification, and minichromosome maintenance protein 5 (MCM5) protein expression were detected...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228317/leukocyte-telomere-length-at-birth-and-during-the-early-life-of-hiv-exposed-uninfected-children-following-in-utero-exposure-to-antiretrovirals
#2
Abhinav Ajaykumar, Hugo Soudeyns, Fatima Kakkar, Jason Brophy, Ari Bitnun, Ariane Alimenti, Arianne Y K Albert, Deborah M Money, Hélène C F Côté
Background: Maternal combination antiretroviral therapy (cART) during pregnancy could impact the health of HIV-exposed uninfected (HEU) children, as some antiretrovirals cross the placenta and can inhibit telomerase. Our objective was to compare HEU and HIV-unexposed uninfected (HUU) leukocyte telomere length (LTL) at birth and in early life, and investigate any relationship with cART exposure. Methods: HEU and HUU children's blood LTL was compared cross-sectionally at birth, and during the first three years of life...
December 8, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29228254/structural-and-functional-analysis-of-an-ob-fold-in-human-ctc1-implicated-in-telomere-maintenance-and-bone-marrow-syndromes
#3
Prashanth K Shastrula, Cory T Rice, Zhuo Wang, Paul M Lieberman, Emmanuel Skordalakes
The human CST (Ctc1, Stn1 and Ten1) complex binds the telomeric overhang and regulates telomere length by promoting C-strand replication and inhibiting telomerase-dependent G-strand synthesis. Structural and biochemical studies on the human Stn1 and Ten1 complex revealed its mechanism of assembly and nucleic acid binding. However, little is known about the structural organization of the multi-domain Ctc1 protein and how each of these domains contribute to telomere length regulation. Here, we report the structure of a central domain of human Ctc1...
December 8, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29224245/regulation-of-c-myc-transcriptional-activity-by-transforming-growth-factor-beta-1-stimulated-clone-22
#4
Ling Zheng, Hiroyuki Suzuki, Yuka Nakajo, Akinobu Nakano, Mitsuyasu Kato
C-MYC stimulates cell proliferation through the suppression of cyclin-dependent kinase (CDK) inhibitors including P15 (CDKN2B) and P21 (CDKN1A). It also activates E-box-mediated transcription of various target genes including telomerase reverse transcriptase (TERT) that is involved in cellular immortality and tumorigenesis. Transforming growth factor-beta 1 (TGF-β1)-stimulated clone 22 (TSC-22/TSC22D1) encodes a highly conserved leucine zipper protein that is induced by various stimuli, including TGF-β. TSC-22 inhibits cell growth in mammalian cells and in Xenopus embryos...
December 10, 2017: Cancer Science
https://www.readbyqxmd.com/read/29222734/tert-promoter-hotspot-mutations-in-breast-cancer
#5
Tatsunori Shimoi, Masayuki Yoshida, Yuka Kitamura, Tomomi Yoshino, Asuka Kawachi, Akihiko Shimomura, Emi Noguchi, Mayu Yunokawa, Kan Yonemori, Chikako Shimizu, Takayuki Kinoshita, Koichi Ichimura, Takahiro Fukuda, Yasuhiro Fujiwara, Kenji Tamura
BACKGROUND: Telomerase reverse transcriptase (TERT) promoter mutations have been discovered in solid and hematological malignancies, where they reflect TERT activation and cell-cycle progression. In melanoma, glioma, and thyroid cancers, TERT promoter mutations are associated with a poor prognosis. However, no studies have evaluated the prevalence and prognostic significance of TERT promoter mutations in breast cancer. METHODS: We analyzed TERT promoter hotspot mutations (C228T and C250T) using direct sequencing of DNA from 319 tumor tissues...
December 8, 2017: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/29221209/direct-hpv-e6-myc-interactions-induce-histone-modifications-pol-ii-phosphorylation-and-htert-promoter-activation
#6
Yiyu Zhang, Aleksandra Dakic, Renxiang Chen, Yuhai Dai, Richard Schlegel, Xuefeng Liu
Human Papillomavirus Viruses (HPVs) are associated with the majority of human cervical and anal cancers and 10-30% of head and neck squamous carcinomas. E6 oncoprotein from high risk HPVs interacts with the p53 tumor suppressor protein to facilitate its degradation and increases telomerase activity for extending the life span of host cells. We published previously that the Myc cellular transcription factor associates with the high-risk HPV E6 protein in vivo and participates in the transactivation of the hTERT promoter...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29221158/epigenetic-silencing-of-mir-296-and-mir-512-ensures-htert-dependent-apoptosis-protection-and-telomere-maintenance-in-basal-type-breast-cancer-cells
#7
Roberto Dinami, Valentina Buemi, Rosanna Sestito, Antonina Zappone, Yari Ciani, Miguel Mano, Eleonora Petti, Andrea Sacconi, Giovanni Blandino, Mauro Giacca, Silvano Piazza, Roberta Benetti, Stefan Schoeftner
The catalytic subunit of the telomerase complex, hTERT, ensures unlimited proliferative potential of cancer cells by maintaining telomere function and protecting from apoptosis. Using a miRNA screening approach we identified miR-296-5p and miR-512-5p as miRNAs that target hTERT in breast cancer cells. Ectopic miR-296-5p and miR-512-5p reduce telomerase activity, drive telomere shortening and cause proliferation defects by enhancing senescence and apoptosis in breast cancer cells. In line with the relevance of hTERT expression for human cancer we found that miR-296-5p and miR-512-5p expression is reduced in human breast cancer...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29216888/telomere-heterogeneity-linked-to-metabolism-and-pluripotency-state-revealed-by-simultaneous-analysis-of-telomere-length-and-rna-seq-in-the-same-human-embryonic-stem-cell
#8
Hua Wang, Kunshan Zhang, Yifei Liu, Yudong Fu, Shan Gao, Peng Gong, Haiying Wang, Zhongcheng Zhou, Ming Zeng, Zhenfeng Wu, Yu Sun, Tong Chen, Siguang Li, Lin Liu
BACKGROUND: Telomere length heterogeneity has been detected in various cell types, including stem cells and cancer cells. Cell heterogeneity in pluripotent stem cells, such as embryonic stem cells (ESCs), is of particular interest; however, the implication and mechanisms underlying the heterogeneity remain to be understood. Single-cell analysis technology has recently been developed and effectively employed to investigate cell heterogeneity. Yet, methods that can simultaneously measure telomere length and analyze the global transcriptome in the same cell have not been available until now...
December 8, 2017: BMC Biology
https://www.readbyqxmd.com/read/29216371/fission-yeast-ccq1-is-a-modulator-of-telomerase-activity
#9
Christine A Armstrong, Vera Moiseeva, Laura C Collopy, Siân R Pearson, Tomalika R Ullah, Shidong T Xi, Jennifer Martin, Shaan Subramaniam, Sara Marelli, Hanna Amelina, Kazunori Tomita
Shelterin, the telomeric protein complex, plays a crucial role in telomere homeostasis. In fission yeast, telomerase is recruited to chromosome ends by the shelterin component Tpz1 and its binding partner Ccq1, where telomerase binds to the 3' overhang to add telomeric repeats. Recruitment is initiated by the interaction of Ccq1 with the telomerase subunit Est1. However, how telomerase is released following elongation remains to be established. Here, we show that Ccq1 also has a role in the suppression of telomere elongation, when coupled with the Clr4 histone H3 methyl-transferase complex and the Clr3 histone deacetylase and nucleosome remodelling complex, SHREC...
December 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29214501/mouse-embryonic-fibroblast-mef-bmp4-conditioned-medium-enhanced-multipotency-of-human-dental-pulp-cells
#10
Lu Liu, Zhengjun Peng, Zhezhen Xu, Haoquan Huang, Xi Wei
Dental pulp cells (DPCs) are valuable cell source for dental regeneration, albeit their application is restricted by limited pluripotency due to current culture condition. Mouse embryonic fibroblasts (MEFs) are served as feeder layer to maintain undifferentiated state of iPSCs and ESCs with long-term in vitro culture. Bone morphogenetic protein 4 (BMP4) plays an important role in the regulation of undifferentiated state and lineage commitment of cells through modulation of microenvironment. However, so far little was known how micro environment affect the multipotency of dental derived cells...
December 6, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29211436/quantitative-determination-of-telomerase-activity-by-combining-fluorescence-correlation-spectroscopy-with-telomerase-repeat-amplification-protocol-trap
#11
Di Su, Xiangyi Huang, Chaoqing Dong, Jicun Ren
Telomerase is a key enzyme for maintaining the telomere length and is regarded as a versatile cancer biomarker and a potential drug target due to its important role in cancer and aging. It is necessary to develop a sensitive and reliable method for detection of telomerase activity due to its very low level in cells. In this article, we propose an ultra-sensitive and robust method for quantitative determination of telomerase activity by combining single molecule fluorescence correlation spectroscopy (FCS) with telomerase repeat amplification protocol (TRAP)...
December 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29211306/telomerase-reverse-transcriptase-promoter-alterations-across-cancer-types-as-detected-by-next-generation-sequencing-a-clinical-and-molecular-analysis-of-423-patients
#12
Maria Schwaederle, Nithya Krishnamurthy, Gregory A Daniels, David E Piccioni, Santosh Kesari, Paul T Fanta, Richard B Schwab, Sandip P Patel, Barbara A Parker, Razelle Kurzrock
BACKGROUND: Telomerase reverse transcriptase (TERT) promoter mutations that may affect telomerase activity have recently been described in human malignancies. The purpose of this study was to investigate the clinical correlates of TERT promoter abnormalities in a large cohort of patients with diverse cancers. METHODS: This study analyzed TERT promoter alterations and clinical characteristics of 423 consecutive patients for whom molecular testing by next-generation sequencing was performed between August 2014 and July 2015...
December 6, 2017: Cancer
https://www.readbyqxmd.com/read/29207187/macrophage-migration-inhibitory-factor-rescues-mesenchymal-stem-cells-from-doxorubicin-induced-senescence-though-the-pi3k-akt-signaling-pathway
#13
Wenzheng Xia, Meng Hou
Doxorubicin (DOXO), an anthracycline antibiotic, is a commonly used anticancer drug. Despite its widespread usage, the therapeutic effects of DOXO are limited by its cardiotoxicity. Mesenchymal stem cell (MSC)-based therapies have had positive outcomes in the treatment of DOXO-induced cardiac damage; however, DOXO exerts toxic effects on MSCs, decreasing the effectiveness of MSC therapy. Macrophage migration inhibitory factor (MIF) promotes MSC survival and rejuvenation, and thus is a promising candidate to protect MSCs against DOXO-induced injury...
November 23, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29207090/long-intergenic-non%C3%A2-coding-rna%C3%A2-p21-mediates-cardiac-senescence-via-the-wnt-%C3%AE-%C3%A2-catenin-signaling-pathway-in-doxorubicin-induced-cardiotoxicity
#14
Zhongdong Xie, Wenzheng Xia, Meng Hou
Doxorubicin (Dox)-induced cardiotoxicity has been a well‑known phenomenon to clinicians and scientists for decades. It has been confirmed that Dox‑dependent cardiotoxicity is accompanied by cardiac cellular senescence. However, the molecular mechanisms underlying Dox cardiotoxicity remains to be fully elucidated. Long non‑coding (lnc) RNAs regulate gene transcription and the fate of post‑transcriptional mRNA, which affects a broad range of age‑associated physiological and pathological conditions, including cardiovascular disease and cellular senescence...
November 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29205098/platelet-derived-growth-factor-receptor-alpha-expressing-cardiac-progenitor-cells-can-be-derived-from-previously-cryopreserved-human-heart-samples
#15
Thi Yen Loan Le, Hilda Pickett, Cristobal Dos Remedios, Pasquale Barbaro, Eddy Kizana, James Chong
Cardiac progenitor cells (CPCs) are being developed as a promising treatment for heart failure. Although clinical trials have predominantly used donor cardiac biopsies to derive CPCs, a better solution could be to use previously cryopreserved human heart tissue. This would enable timely and convenient access to healthy and young heart samples for CPC production. However, few studies have attempted to isolate CPCs from previously cryopreserved heart tissue. In this study, we isolated CPCs from eight non-diseased human heart samples previously cryopreserved as part of the Sydney Heart Bank...
December 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29203363/mechanism-of-action-of-g-quadruplex-forming-oligonucleotide-homologous-to-the-telomere-overhang-in-melanoma
#16
Gagan Chhabra, Luke Wojdyla, Mark Frakes, Zachary Schrank, Brandon Leviskas, Marko Ivancich, Pooja Vinay, Ramesh Ganapathy, Benjamin E Ramirez, Neelu Puri
T-oligo, a guanine-rich oligonucleotide (GRO) homologous to the 3'-telomeric overhang of telomeres, elicits potent DNA-damage responses in melanoma cells; however, its mechanism of action is largely unknown. GROs can form G-quadruplexes (G4) which are stabilized by the hydrogen-bonding of guanine residues. In this study, we confirmed the G4-forming capabilities of T-oligo using non-denaturing PAGE, NMR and immunofluorescence. Using an anti-G-quadruplex antibody (BG4) we showed that T-oligo can form G4 in the nuclei of melanoma cells...
December 1, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29202008/suppression-of-oncolytic-adenovirus-mediated-hepatotoxicity-by-liver-specific-inhibition-of-nf-%C3%AE%C2%BAb
#17
Mitsuhiro Machitani, Fuminori Sakurai, Keisaku Wakabayashi, Kosuke Nakatani, Masashi Tachibana, Nobuyuki Kato, Toshiyoshi Fujiwara, Hiroyuki Mizuguchi
Telomerase-specific replication-competent adenoviruses (Ads), i.e., TRADs, which possess an E1 gene expression cassette driven by the human telomerase reverse transcriptase promoter, are promising agents for cancer treatment. However, even though oncolytic Ads, including TRAD, are intratumorally administered, they are disseminated from the tumor to systemic circulation, causing concern about oncolytic Ad-mediated hepatotoxicity (due mainly to leaky expression of Ad genes in liver). We reported that inhibition of nuclear factor-κB (NF-κB) leads to the suppression of replication-incompetent Ad vector-mediated hepatotoxicity via reduction of the leaky expression of Ad genes in liver...
December 15, 2017: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/29198328/the-role-of-nuclear-factor-kappa-b-signaling-in-human-cervical-cancer
#18
REVIEW
Sam Tilborghs, Jerome Corthouts, Yannick Verhoeven, David Arias, Christian Rolfo, Xuan Bich Trinh, Peter A van Dam
Background The Nuclear Factor kappaB (NF-kB) family consists of transcription factors that play a complex and essential role in the regulation of immune responses and inflammation. NF-kB has recently generated considerable interest as it has been implicated in human cancer initiation, progression and resistance to treatment. In the present comprehensive review the different aspects of NF-kB signaling in the carcinogenesis of cancer of the uterine cervix are discussed. NF-kB functions as part of a network, which determines the pattern of its effects on the expression of several other genes (such as crosstalks with reactive oxygen species, p53, STAT3 and miRNAS) and thus its function...
December 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/29198322/tert-promoter-mutation-and-its-interaction-with-idh-mutations-in-glioma-combined-tert-promoter-and-idh-mutations-stratifies-lower-grade-glioma-into-distinct-survival-subgroups-a-meta-analysis-of-aggregate-data
#19
REVIEW
Huy Gia Vuong, Ahmed M A Altibi, Uyen N P Duong, Hanh T T Ngo, Thong Quang Pham, Aden Ka-Yin Chan, Chul-Kee Park, Kar-Ming Fung, Lewis Hassell
The clinical significance of telomerase reverse transcriptase (TERT) promoter mutation in glioma remains unclear. The aim of our meta-analysis is to investigate the prognostic impact TERT promoter mutation in glioma patients and its interaction with other molecular markers, particularly Isocitrate Dehydrogenase (IDH) mutation from aggregate level data. Relevant articles were searched in four electronic databases including PubMed, Scopus, Web of Science and Virtual Health Library. Pooled HRs were calculated using random effect model weighted by inverse variance method...
December 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/29198089/-exosomes-as-mediators-of-intercellular-communication-the-cancer-and-telomerase-connection
#20
REVIEW
Orit Uziel, Anna Gutkin, Einat Beery, Meir Lahav
Exosomes, nano-vesicles secreted from all types of cells in the human body, function as inter-cell communicators. This role of exosomes is fulfilled by their specific content, dependent on the origin of donor cells from which they are secreted. Exosomes contain a plethora of nucleic acids (DNA, RNA and micro RNA), proteins and lipids. These molecules are packed in the donor cells into the exosomes which are subsequently secreted and transferred through various body fluids into the target cells which may be located far from the donor cells...
November 2017: Harefuah
keyword
keyword
6048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"