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https://www.readbyqxmd.com/read/29342276/a-predictive-quantitative-model-of-spiking-activity-and-stimulus-secretion-coupling-in-oxytocin-neurons
#1
Jorge Maícas-Royo, Gareth Leng, Duncan J MacGregor
Oxytocin neurons of the rat hypothalamus project to the posterior pituitary where they secrete their products into the bloodstream. The pattern and quantity of that release depends on the afferent inputs to the neurons, on their intrinsic membrane properties, and on non-linear interactions between spiking activity and exocytosis: a given number of spikes will trigger more secretion when they arrive close together. Here we present a quantitative computational model of oxytocin neurons that can replicate the results of a wide variety of published experiments...
January 12, 2018: Endocrinology
https://www.readbyqxmd.com/read/29342156/specialized-attachment-structure-of-the-fish-pathogenic-oomycete-saprolegnia-parasitica
#2
Svetlana Rezinciuc, Jose Vladimir Sandoval-Sierra, Yolanda Ruiz-León, Pieter van West, Javier Diéguez-Uribeondo
The secondary cysts of the fish pathogen oomycete Saprolegnia parasitica possess bundles of long hooked hairs that are characteristic to this economically important pathogenic species. Few studies have been carried out on elucidating their specific role in the S. parasitica life cycle and the role they may have in the infection process. We show here their function by employing several strategies that focus on descriptive, developmental and predictive approaches. The strength of attachment of the secondary cysts of this pathogen was compared to other closely related species where bundles of long hooked hairs are absent...
2018: PloS One
https://www.readbyqxmd.com/read/29341923/purification-chitooligosaccharide-binding-properties-and-thermal-stability-of-cia24-a-new-pp2-like-phloem-exudate-lectin-from-ivy-gourd-coccinia-indica
#3
Kishore Babu Bobbili, Debparna Datta, Saradamoni Mondal, Sirilatha Polepalli, Michael Mormann, Musti J Swamy
PP2-like chitin binding phloem exudate lectins, abundant in the sieve tube of cucurbits, have been implicated to play key roles in wound sealing and antipathogenic responses of the plant. Here we report the affinity purification, macromolecular characterization and carbohydrate binding properties of a new chitooligosaccharide-specific lectin from the phloem exudate of ivy gourds (Coccinia indica). The protein, CIA24, has a subunit mass of 24 kDa. Partial sequence analysis indicated that CIA24 exhibits high homology with CIA17 and other Cucurbitaceae PP2 proteins whereas CD spectroscopic studies suggested that β-sheets constitute the predominant secondary structure...
January 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29341665/can-hail-and-rain-nucleate-cloud-droplets
#4
Prasanth Prabhakaran, Stephan Weiss, Alexei Krekhov, Alain Pumir, Eberhard Bodenschatz
We present results from moist convection in a mixture of pressurized sulfur hexafluoride (liquid and vapor), and helium (gas) to model the wet and dry components of the Earth's atmosphere. To allow for homogeneous nucleation, we operate the experiment close to critical conditions. We report on the nucleation of microdroplets in the wake of large cold liquid drops falling through the supersaturated atmosphere and show that the homogeneous nucleation is caused by isobaric cooling of the saturated sulfur hexafluoride vapor...
September 22, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29341402/isotope-substitution-of-promiscuous-alcohol-dehydrogenase-reveals-origin-of-substrate-preference-in-transition-state
#5
Rudolf K Allemann, J Javier Ruiz-Pernia, Louis Luk, Inaki Tunon, Vicent Moliner, Enas Behiry
The origin of substrate preference in promiscuous enzymes was investigated by enzyme isotope labeling of the alcohol dehydrogenase from Geobacillus stearothermophilus (BsADH). At physiological temperature, protein dynamic coupling to the reaction coordinate was insignificant. However, the extent of dynamic coupling was highly substrate-dependent at lower temperatures. For benzyl alcohol, an enzyme isotope effect larger than unity was observed, whereas the enzyme isotope effect was close to unity for isopropanol...
January 17, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29341388/venous-anastomosis-by-piggyback-technique-to-avoid-twisting-of-the-pediatric-en-bloc-kidney-grafts
#6
Jun Li, Raphael Schild, Jochen Herrmann, Jun Oh, Lutz Fischer, Martina Koch
This is the first report of using piggyback technique for the venous anastomosis in two pediatric recipients of small en bloc kidneys, which was found to be effective to avoid twisting of the grafts and vessels. The donors were aged 2 and 3 years with a body weight of 17 and 20 kg. The recipient age was 14 and 16 years with a body weight of 42 and 54 kg. The implantation was done extraperitoneally in the right iliac fossa. The donor's inferior vena cava was anastomosed to the recipient's distal caval vein side-to-side using 6-0 polydioxanone running suture as the piggyback technique, initially dealing with the short vena cava graft in the first case...
January 16, 2018: Pediatric Transplantation
https://www.readbyqxmd.com/read/29340922/prevention-of-internal-hernia-during-robotic-total-gastrectomy-for-gastric-cancer
#7
Toshiyasu Ojima, Masaki Nakamura, Mikihito Nakamori, Keiji Hayata, Masahiro Katsuda, Toshiaki Tsuji, Shimpei Maruoka, Hiroki Yamaue
BACKGROUND: Postoperative internal hernia (IH) is a potentially life-threatening acute protrusion of viscus through an iatrogenic mesenteric defect. In our retrospective study of 1943 consecutive gastric cancer (GC) patients who had undergone surgery, the incidence of IH after laparoscopic total gastrectomy (LTG) was 4.9%.1 This high incidence seems to be caused by decreased adhesion formation after LTG. There is no consensus regarding orifice management during robotic total gastrectomy (RTG)...
January 16, 2018: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
https://www.readbyqxmd.com/read/29340799/fully-automated-life-support-an-implementation-and-feasibility-pilot-study-in-healthy-pigs
#8
Wilfried Klingert, Jörg Peter, Christian Thiel, Karolin Thiel, Wolfgang Rosenstiel, Kathrin Klingert, Christian Grasshoff, Alfred Königsrainer, Martin Schenk
BACKGROUND: Automated systems are available in various application areas all over the world for the purpose of reducing workload and increasing safety. However, such support systems that would aid caregivers are still lacking in the medical sector. With respect to workload and safety, especially, the intensive care unit appears to be an important and challenging application field. Whereas many closed-loop subsystems for single applications already exist, no comprehensive system covering multiple therapeutic aspects and interactions is available yet...
January 16, 2018: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/29340784/a-new-flesh-fly-species-diptera-sarcophagidae-parasitic-on-leptodactylid-frogs
#9
Pablo Ricardo Mulieri, Eduardo Federico Schaefer, Marta Inés Duré, Cynthya Elizabeth González
Here we describe a new species of sarcophagid fly reared from larvae found feeding on three species of leptodactylid frogs collected in subtropical Corrientes Province in northeastern Argentina. Our species description is based on adult male and female external morphology and genitalia. Adult males and females of the new species were associated with certainty because all specimens studied were reared from single clutches of larvae on each of three infested frogs. Thus, adult males and females reared from each clutch were siblings that emerged almost simultaneously...
January 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29340768/the-comparison-of-clinical-outcomes-in-patients-with-acute-myocardial-infarction-and-advanced-chronic-kidney-disease-on-chronic-hemodialysis-versus-off-hemodialysis
#10
Naoyuki Akashi, Kenichi Sakakura, Yusuke Watanabe, Masamitsu Noguchi, Yousuke Taniguchi, Kei Yamamoto, Hiroshi Wada, Shin-Ichi Momomura, Hideo Fujita
Acute myocardial infarction (AMI) is more frequently observed in patients with chronic kidney disease (CKD) than in patients without CKD. Initial treatment strategy for AMI includes primary percutaneous coronary intervention (PCI), which requires substantial amount of contrast media. We hypothesized that the clinical outcomes are comparable or worse in patients with AMI and advanced CKD off chronic hemodialysis as compared to patients with AMI and advanced CKD on chronic hemodialysis. The purpose of this study was to compare the clinical outcomes of patients with AMI and advanced CKD on hemodialysis versus off hemodialysis...
January 16, 2018: Heart and Vessels
https://www.readbyqxmd.com/read/29340481/evaluation-of-the-sealing-ability-of-different-root-canal-sealers-a-combined-sem-and-micro-ct-study
#11
Yan Huang, Kaan Orhan, Berkan Celikten, Ayşe Işıl Orhan, Pelin Tufenkci, Semra Sevimay
OBJECTIVE: The purpose of this study was to analyze the ability of multiple compounds to seal the dental tubules using scanning electron microscopy (SEM) and micro-computed tomogra-phy (micro-CT). MATERIAL AND METHODS: Twenty-four single-root human mandibular premolars were selected and instrumented with nickel-titanium rotary file and the final file size was #40/06. They were then randomly allocated into 2 groups, and all samples were filled with single cone gutta-percha (#40/06) and one of the tested sealers (AH Plus and EndoSequence BC sealers)...
January 15, 2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29340390/ultrafast-stimulated-emission-of-nitrophenolates-in-organic-and-aqueous-solutions
#12
N C Michenfelder, H A Ernst, C Schweigert, M Olzmann, A-N Unterreiner
Early-time dynamics of nitroaromatics and its coressponding bases can give valuable insights into photo-induced reactions relevant to atmospheric and environmental processes. In this work, femtosecond broadband absorption spectroscopy between 350 and 700 nm has been applied to explore the ultrafast dynamics of o-, p- and m-nitrophenol anions (NP-) in basic organic and aqueous solution. Excitation at 400 nm promotes these compounds into the first bright electronic singlet state, which is a charge-transfer state...
January 17, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29340267/postherpetic-abdominal-pseudohernia-a-diagnostic-pitfall
#13
Yu Yagi, Takashi Matono, Kenichi Nakamura, Hiroshi Imura
The accurate diagnosis of postherpetic abdominal pseudohernia, the rare complication of herpes zoster, is essential to avoid unnecessary imaging studies or surgery. Close observation and waiting for complete recovery are warranted considering the disease's self-resolving nature and favorable prognosis.
January 2018: Journal of General and Family Medicine
https://www.readbyqxmd.com/read/29340248/depth-and-range-dependent-variation-in-the-performance-of-aquatic-telemetry-systems-understanding-and-predicting-the-susceptibility-of-acoustic-tag-receiver-pairs-to-close-proximity-detection-interference
#14
Stephen R Scherrer, Brendan P Rideout, Giacomo Giorli, Eva-Marie Nosal, Kevin C Weng
Background: Passive acoustic telemetry using coded transmitter tags and stationary receivers is a popular method for tracking movements of aquatic animals. Understanding the performance of these systems is important in array design and in analysis. Close proximity detection interference (CPDI) is a condition where receivers fail to reliably detect tag transmissions. CPDI generally occurs when the tag and receiver are near one another in acoustically reverberant settings. Here we confirm transmission multipaths reflected off the environment arriving at a receiver with sufficient delay relative to the direct signal cause CPDI...
2018: PeerJ
https://www.readbyqxmd.com/read/29340217/translational-cross-activation-of-the-encapsidated-rna-of-potexviruses
#15
M V Arkhipenko, N A Nikitin, E K Donchenko, O V Karpova, J G Atabekov
We had shown the genomic RNA of potexviruses potato virus X and the alternanthera mosaic virus to be inaccessible in vitro to ribosomes while in intact virion form, but the RNAs can be translationally activated following the binding of movement protein 1 (MP1) to virus particles. Here, we present the results of the follow-up study targeting two more potexvirus species - the Narcissus mosaic virus and the Potato aucuba mosaic virus. We found encapsidated potexviral RNA to share common translational features in vitro and the MP1 to be potent over homological virions of its "own" species and over heterological virions of other species, as well exhibiting selective specificity...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29339603/simultaneous-organic-matter-removal-and-nitrification-of-an-inert-self-supporting-immersed-media-to-upgrade-aerated-lagoons
#16
E Boutet, S Baillargeon, B Patry, P Lessard
A pilot study was performed to evaluate the potential of an inert self-supported immersed fixed film media to upgrade aerated lagoons. Simultaneous organic matter removal and nitrification was assessed under different loading rates and temperatures (near 0 °C) using 12 laboratory-scale reactors operated in parallel. Test results showed that both the temperature and the load have an influence on organic matter effluent concentrations. Effluent quality seemed related to the observed biofilm thickness. Thicker biofilm is believed to have contributed to biomass detachment and increased particulate organic matter concentrations in the effluent...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29339271/early-increase-in-complement-terminal-pathway-activation-marker-sc5b-9-is-predictive-for-the-development-of-thrombotic-microangiopathy-after-stem-cell-transplantation
#17
Orsolya Horváth, Krisztián Kállay, Dorottya Csuka, Blanka Mező, György Sinkovits, Csaba Kassa, Anita Stréhn, Katalin Csordás, János Sinkó, Zoltán Prohászka, Gergely Kriván
Hematopoietic stem cell transplantation- (HSCT-) associated thrombotic microangiopathy (TA-TMA) is a multifactorial complication, and its prediction is largely unresolved.Our aim was to analyze changes of complement profile after HSCT in order to identify potential markers of TA-TMA development. Thirty- three consecutive pediatric patients (9.6 ± 4.4 years old) were included in this study who underwent allogeneic HSCT due to malignant (n=17) or non malignant (n=16) indications. Graft versus host disease (GVHD) was diagnosed using Glucksberg criteria, viral reactivation was monitored, five different TA-TMA diagnostic criteria were applied and all important clinical and laboratory parameters of TA-TMA activity were registered...
January 12, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29339213/nrp-2-in-tumor-lymphangiogenesis-and-lymphatic-metastasis
#18
Jingwen Wang, Yuhong Huang, Jun Zhang, Boyi Xing, Wei Xuan, Honghai Wang, He Huang, Jiayu Yang, Jianwu Tang
Neuropilin-2 (NRP-2) not only functions as a receptor for semaphorins, a family of neural axon guidance factors, but also interacts with VEGFs, a family of vascular endothelial growth factors. As an independent receptor or a co-receptor, NRP-2 binds to ligands VEGF-C/D, activates the VEGF-C/D-NRP-2 signaling axis, and further regulates lymphangiogenesis-associated factors in both lymphatic endothelial cells (LECs) and some tumor cells during tumor progression. Via VEGF-C/D-NRP-2 axis, NRP-2 induces LEC proliferation, reconstruction and lymphangiogenesis and subsequently promotes tumor cell migration, invasion and lymphatic metastasis...
January 12, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29339097/safety-and-preliminary-efficacy-of-venetoclax-with-decitabine-or-azacitidine-in-elderly-patients-with-previously-untreated-acute-myeloid-leukaemia-a-non-randomised-open-label-phase-1b-study
#19
Courtney D DiNardo, Keith W Pratz, Anthony Letai, Brian A Jonas, Andrew H Wei, Michael Thirman, Martha Arellano, Mark G Frattini, Hagop Kantarjian, Relja Popovic, Brenda Chyla, Tu Xu, Martin Dunbar, Suresh K Agarwal, Rod Humerickhouse, Mack Mabry, Jalaja Potluri, Marina Konopleva, Daniel A Pollyea
BACKGROUND: Elderly patients (aged ≥65 years) with acute myeloid leukaemia have poor outcomes and no effective standard-of-care therapy exists. Treatment with hypomethylating agents such as azacitidine and decitabine is common, but responses are modest and typically short-lived. The oral anti-apoptotic B-cell lymphoma 2 protein inhibitor, venetoclax, has shown promising single-agent activity in patients with relapsed or refractory acute myeloid leukaemia and preclinical data suggested synergy between hypomethylating agents and venetoclax, which led to this combination phase 1b study...
January 12, 2018: Lancet Oncology
https://www.readbyqxmd.com/read/29339081/disorder-to-order-transitions-in-the-molten-globule-like-golgi-reassembly-and-stacking-protein
#20
Luís F S Mendes, Luis G M Basso, Patricia S Kumagai, Raquel Fonseca-Maldonado, Antonio J Costa-Filho
BACKGROUND: Golgi Reassembly and Stacking Proteins (GRASPs) are widely spread among eukaryotic cells (except plants) and are considered as key components in both the stacking of the Golgi cisternae and its lateral connection. Furthermore, GRASPs were also proved essential in the unconventional secretion pathway of several proteins, even though the mechanism remains obscure. It was previously observed that the GRASP homolog in Cryptococcus neoformans has a molten globule-like behavior in solution...
January 12, 2018: Biochimica et Biophysica Acta
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