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aminolevulinic acid

Soo-Jin Bae, Dae-Sic Lee, Vladimir Berezin, Uk Kang, Keun-Ho Lee
AIM: The aim of this study was to develop a novel optical imaging system for detecting protoporphyrin IX (PpIX) autofluorescence, to prove that PpIX autofluorescence is as useful as 5-aminolevulinic acid (5-ALA)-induced fluorescence for detecting and localizing cervical cancer, and to monitor the change in PpIX autofluorescence or induced PpIX fluorescence before, during, and after photodynamic therapy (PDT). METHODS: TC-1 cells - highly tumorigenic cells immortalized using human papillomavirus type 16 proteins E6 and E7 - were subcutaneously grafted into the thighs of nude mice...
October 17, 2016: Journal of Obstetrics and Gynaecology Research
Sarah Qureshi, Jennifer Y Lin
Aminolevulinic acid (ALA) photodynamic therapy (PDT) is an emerging modality in the treatment of acne. While ablative fractional lasers have been used to enhance drug delivery into the epidermis, recent evidence suggests that non-ablative fractional photothermolysis may also improve uptake of ALA. We explored the use of non-ablative 1550 nm laser as a safe alternative in the delivery of ALA prior to red-light PDT for refractory inflammatory and cystic acne. Subjects referred for treatment of acne refractory to several topical and oral regimens, including isotretinoin, were pre-treated with non-ablative fractional photothermolysis (NAFP)...
October 14, 2016: Lasers in Medical Science
Feryal Al-Saber, Waleed Aldosari, Mariam Alselaiti, Hesham Khalfan, Ahmed Kaladari, Ghulam Khan, George Harb, Riyadh Rehani, Sizuka Kudo, Aya Koda, Tohru Tanaka, Motowo Nakajima, Abdulla Darwish
Type 2 diabetes mellitus is prevalent especially in Gulf countries and poses serious long-term risks to patients. A multifaceted treatment approach can include nutritional supplements with antioxidant properties such as 5-aminolevulinic acid (5-ALA) with sodium ferrous citrate (SFC). This prospective, randomized, single-blind, placebo-controlled, dose escalating pilot clinical trial assessed the safety of 5-ALA with SFC at doses up to 200 mg 5-ALA/229.42 mg SFC per day in patients living in Bahrain with type 2 diabetes mellitus that was uncontrolled despite the use of one or more antidiabetic drugs...
2016: Journal of Diabetes Research
Kara M Harmatys, Anthony J Musso, Kasey J Clear, Bradley D Smith
Administration of exogenous 5-aminolevulinic acid (5-ALA) to cancerous tissue leads to intracellular production of photoactive protoporphyrin IX, a biosynthetic process that enables photodynamic therapy and fluorescence-guided surgery of cancer. Cell uptake of 5-ALA is limited by its polar structure and there is a need for non-toxic chemical additives that can enhance its cell permeation. Two zinc-bis(dipicolylamine) (ZnBDPA) compounds were evaluated for their ability to promote uptake of 5-ALA into Chinese Hamster Ovary (CHO-K1) cells and produce protoporphyrin IX...
October 7, 2016: Photochemical & Photobiological Sciences
Hirotada Nishie, Hiromi Kataoka, Shigenobu Yano, Jun-Ichi Kikuchi, Noriyuki Hayashi, Atsushi Narumi, Akihiro Nomoto, Eiji Kubota, Takashi Joh
Photodynamic therapy (PDT) exploits light interactions and photosensitizers to induce cytotoxic reactive oxygen species. Photodynamic diagnosis (PDD) uses the phenomenon of photosensitizer emitting fluorescence to distinguish some tumors from normal tissue. The standard photosensitizer used for PDD is 5-aminolevulinic acid (5-ALA), although it is not entirely satisfactory. We previously reported glucose-conjugated chlorin (G-chlorin) as a more effective photosensitizer than another widely used photosensitizer, talaporfin sodium (TS); however, G-chlorin is hydrophobic...
September 30, 2016: Oncotarget
Mingyi Zhao, Ping Zhu, Masayuki Fujino, Yoshiaki Nishio, Jimei Chen, Hidenori Ito, Kiwamu Takahashi, Motowo Nakajima, Tohru Tanaka, Lingling Zhao, Jian Zhuang, Xiao-Kang Li
BACKGROUND: Hypoxia causes cardiac disease via oxidative stress and mitochondrial dysfunction. 5-Aminolevulinic acid in combination with sodium ferrous citrate (ALA/SFC) has been shown to up-regulate heme oxygenase-1 (HO-1) and decrease macrophage infiltration and renal cell apoptosis in renal ischemia injury mice. However, its underlying mechanism remains largely unknown. The aim of this study was to investigate whether ALA/SFC could protect cardiomyocytes from hypoxia-induced apoptosis by autophagy via HO-1 signaling...
October 28, 2016: Biochemical and Biophysical Research Communications
Jina Wu, Ye Lin, Huan Li, Qiao Jin, Jian Ji
5-Aminolevulinic acid (ALA) is a FDA-approved photodynamic therapy (PDT) precursor of protoporphyrin IX (PpIX) used for treating various cancers. However, the internalization of ALA is a big challenge due to its hydrophilic nature and low specificity to cancer cells. In this work, ALA conjugated prodrug nanoparticles were prepared by conjugation of thiolated stealth peptide sequence CPPPPEKEKEKEKEKEDGR and hydrazone-containing ALA to gold nanoparticles (AuNPs). Remarkable anti-fouling ability of ALA prodrug nanoparticles in complex environment was achieved owing to the zwitterionic stealth peptide sequence EKEKEKEKEK...
January 1, 2017: Journal of Colloid and Interface Science
Sameer H Halani, D Cory Adamson
Surgical resection is typically the first line of treatment for gliomas. However, the neurosurgeon faces a major challenge in achieving maximal resection in high-grade gliomas as these infiltrative tumors make it difficult to discern tumor margins from normal brain with conventional white-light microscopy alone. To aid in resection of these infiltrative tumors, fluorescence-guided surgery has gained much popularity in intraoperative visualization of malignant gliomas, with 5-aminolevulinic acid (5-ALA) leading the way...
2016: OncoTargets and Therapy
E V Filonenko, A D Kaprin, B Y A Alekseev, O I Apolikhin, E K Slovokhodov, V I Ivanova-Radkevich, A N Urlova
The results of multicenter prospective trial for efficacy of combined modality treatment: transurethral resection (TUR) + photodynamic therapy (PDT) with alasens for bladder cancer are represented in the article. Trials were organized by Research Institute of Organic Intermediates and Dyes and conducted according to clinical protocol approved by Ministry of Health of Russia, at the sites of leading Russian cancer clinical centers. The trial included 45 subjects with verified diagnosis of non-muscle-invasive bladder cancer...
September 23, 2016: Photodiagnosis and Photodynamic Therapy
Jelena Barbaric, Rachel Abbott, Pawel Posadzki, Mate Car, Laura H Gunn, Alison M Layton, Azeem Majeed, Josip Car
BACKGROUND: Acne vulgaris is a very common skin problem that presents with blackheads, whiteheads, and inflamed spots. It frequently results in physical scarring and may cause psychological distress. The use of oral and topical treatments can be limited in some people due to ineffectiveness, inconvenience, poor tolerability or side-effects. Some studies have suggested promising results for light therapies. OBJECTIVES: To explore the effects of light treatment of different wavelengths for acne...
September 27, 2016: Cochrane Database of Systematic Reviews
Xiaoli Yu, Haiying Jin, Xuelian Cheng, Qian Wang, Qingsheng Qi
5-Aminolevulinic acid (ALA), the committed intermediate of the heme biosynthetic pathway, attracts close attention among researchers because of its potential applications to cancer treatment and agriculture. Overexpression of heterologous hemA and hemL, which encode glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase, respectively, in Corynebacterium glutamicum produces ALA, although whether ALA accumulation causes unintended effects on the host is unknown. Here we used an integrated systems approach to compare global transcriptional changes induced by the expression of hemA and hemL...
November 2016: Microbiological Research
Aliasgar V Moiyadi, Prakash Shetty, Epari Sridhar
RATIONALE: Subventricular zone (SVZ) involvement has been proposed as an adverse prognostic factor in glioblastomas (GBM). The true extent of ventricular involvement at surgery is often difficult to establish and is poorly studied. Tumour fluorescence provides us with an exciting opportunity to interrogate tumour extent intraoperatively. METHODS: We conducted a retrospective analysis of all cases of GBMs operated using aminolevulinic acid-induced fluorescence and analyzed radiological SVZ involvement alongwith the incidence of ventricular entry at surgery, ependymal fluorescence and histological correlation of the ependymal involvement...
September 20, 2016: British Journal of Neurosurgery
Yin-E Hu, Shu-Fang Dai, Bin Wang, Wei Qu, Jun-Ling Gao
OBJECTIVE: To evaluate the therapeutic effects of combined 5-aminolevulinic acid (ALA) and photodynamic therapy (PDT) on genital warts and the safety. METHODS: One hundred ten patients with genital warts who were treated in our hospital from June 2013 to October 2014 were selected. The warts and affected parts were disinfected with benzalkonium bromide solution, and the warts were covered with absorbent cotton that had already been added freshly prepared 20% ALA solution, packaged and fixed...
July 2016: Pakistan Journal of Medical Sciences Quarterly
María Jesús Suárez Valladares, Manuel Ángel Rodríguez Prieto, Ricard Serra Llusà
Photodynamic therapy is a common treatment in dermatology for several diseases, its main limitation is the penetration of photosensitizer and light in the tissue. However intralesional photodynamic therapy is a new technique where the photosensitizer and sometimes a 630nm laser beam are applied inside the lesión, so it allows achieve deeper penetration. It has been used to treat basal cell carcinoma, hidradenitis suppurativa or keloids.
September 16, 2016: Photodiagnosis and Photodynamic Therapy
Vladislav Pavlov, David Meyronet, Vincent Meyer-Bisch, Xavier Armoiry, Brian Pikul, Chloé Dumot, Pierre-Aurelien Beuriat, Francesco Signorelli, Jacques Guyotat
BACKGROUND: The management of gliomas is based on precise histologic diagnosis. The tumor tissue can be obtained during open surgery or via stereotactic biopsy. Intraoperative tissue imaging could substantially improve biopsy precision and, ultimately, the extent of resection. OBJECTIVE: To show the feasibility of intraoperative in vivo probe-based confocal laser endomicroscopy (pCLE) in surgery and biopsy of gliomas. METHODS: In our prospective observational study, 9 adult patients were enrolled between September 2014 and January 2015...
October 2016: Neurosurgery
Masumi Nishimura, Yasutoshi Murayama, Kyoichi Harada, Yosuke Kamada, Ryo Morimura, Hisashi Ikoma, Daisuke Ichikawa, Hitoshi Fujiwara, Kazuma Okamoto, Eigo Otsuji
UNLABELLED: Backgtound/Aim: Since hepatocellular carcinoma (HCC) has a high recurrence rate, accurate diagnosis of its location and curative resection is important to improve survival. This study evaluated the utility of photodynamic diagnosis (PDD) using 5-aminolevulinic acid (5-ALA) for HCC. MATERIALS AND METHODS: We used two human hepatoma cell lines (HuH-7 and Hep G2). Cells were treated with 5-ALA for 4 h. 5-ALA-induced fluorescence was then examined under a fluorescence microscope...
September 2016: Anticancer Research
Karina Paci, Rebecca Todd Bell, Beth Goldstein
No abstract text is available yet for this article.
August 2016: Cutis; Cutaneous Medicine for the Practitioner
Jonathan M Fahey, Joseph V Emmer, Witold Korytowski, Neil Hogg, Albert W Girotti
Gliomas are aggressive brain tumors that are resistant to conventional chemotherapy and radiotherapy. Much of this resistance is attributed to endogenous nitric oxide (NO). Recent studies revealed that 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT) has advantages over conventional treatments for glioblastoma. In this study, we used an in vitro model to assess whether NO from glioblastoma cells can interfere with ALA-PDT. Human U87 and U251 cells expressed significant basal levels of neuronal NO synthase (nNOS) and its inducible counterpart (iNOS)...
September 8, 2016: Photochemistry and Photobiology
Alexander I Rolevich, Alexander G Zhegalik, Andrey A Mokhort, Alexander A Minich, Vladimir Yu Vasilevich, Sergey L Polyakov, Sergey A Krasny, Oleg G Sukonko
OBJECTIVES: To assess the efficacy of two treatment options for non-muscle-invasive bladder cancer (NMIBC): (1) transurethral resection (TUR) guided by fluorescence cystoscopy (FC) with the use of 5-aminolevulinic acid (5-ALA) and (2) single early instillation of doxorubicin in a single-center open-label prospective randomized study with a 2 × 2 factorial design. PATIENTS AND METHODS: Patients with clinical suspicion of primary or recurrent NMIBC were randomized into four study arms: FC-assisted TUR with 5-ALA and single instillation of doxorubicin, FC-assisted TUR without instillation, TUR in white light (WL) with single instillation of doxorubicin, and WL-TUR only...
September 7, 2016: World Journal of Urology
Zaira Rizopoulos, Kai Matuschewski, Joana Haussig
Malarial parasites have evolved complex regulation of heme supply and disposal to adjust to heme-rich and -deprived host environments. In addition to its own pathway for heme biosynthesis, Plasmodium likely harbors mechanisms for heme scavenging from host erythrocytes. Elaborate compartmentalization of de novo heme synthesis into three subcellular locations, including the vestigial plastid organelle, indicates critical roles in life cycle progression. In this study, we systematically profile the essentiality of heme biosynthesis by targeted gene deletion of enzymes in early steps of this pathway...
September 6, 2016: Infection and Immunity
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