keyword
MENU ▼
Read by QxMD icon Read
search

Trpc3

keyword
https://www.readbyqxmd.com/read/29628897/stanniocalcin-2-regulates-non-capacitative-ca-2-entry-and-aggregation-in-mouse-platelets
#1
Esther López, L Gómez-Gordo, Carlos Cantonero, Nuria Bermejo, Jorge Pérez-Gómez, María P Granados, Gines M Salido, Juan A Rosado Dionisio, Pedro C Redondo Liberal
Stanniocalcin 2 (STC2) is a fish protein that controls body Ca2+ and phosphate metabolism. STC2 has also been described in mammals, and as platelet function highly depends on both extracellular and intracellular Ca2+ , we have explored its expression and function in these cells. STC2-/- mice exhibit shorter tail bleeding time than WT mice. Platelets from STC2-deficient mice showed enhanced aggregation, as well as enhanced Ca2+ mobilization in response to the physiological agonist thrombin (Thr) and the diacylglycerol analog, OAG, a selective activator of the non-capacitative Ca2+ entry channels...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29624776/trpc3-overexpression-and-intervention-in-airway-smooth-muscle-of-ovalbumin-induced-hyperresponsiveness-and-remodeling
#2
Bo-Ming Xu, Jia-Hua Zhang, Jia-Ling Wang, Jun-Hua Xiao
Transient receptor potential canonical channel 3 (TRPC3) proteins function as non-voltage-gated Ca2+ -permeable channels and play divergent roles in many processes of pathophysiology. The purpose of this study was to determine the relationship between TRPC3 expression and airway hyperresponsiveness and remodeling in ovalbumin-induced asthmatic Kunming mice. Mice were sensitized and challenged by ovalbumin to establish asthmatic model. Hematoxylin-eosin staining, hydroxyproline assay and isometric tracheal ring force measurement were used to evaluate airway remodeling and hyperresponsiveness in asthmatic mice...
April 6, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29617457/sur1-trpm4-channel-activation-and-phasic-secretion-of-mmp-9-induced-by-tpa-in-brain-endothelial-cells
#3
Volodymyr Gerzanich, Min Seong Kwon, Seung Kyoon Woo, Alexander Ivanov, J Marc Simard
BACKGROUND: Hemorrhagic transformation is a major complication of ischemic stroke, is linked to matrix metalloproteinase-9 (MMP-9), and is exacerbated by tissue plasminogen activator (tPA). Cerebral ischemia/reperfusion is characterized by SUR1-TRPM4 (sulfonylurea receptor 1-transient receptor potential melastatin 4) channel upregulation in microvascular endothelium. In humans and rodents with cerebral ischemia/reperfusion (I/R), the SUR1 antagonist, glibenclamide, reduces hemorrhagic transformation and plasma MMP-9, but the mechanism is unknown...
2018: PloS One
https://www.readbyqxmd.com/read/29574924/the-effects-of-transient-receptor-potential-channel-trpc-on-airway-smooth-muscle-cell-isolated-from-asthma-model-mice
#4
Xiaoyu Zhang, Zhixin Zhao, Lijun Ma, Yali Guo, Xiaosu Li, Limin Zhao, Cuijie Tian, Xueyi Tang, Dongjun Cheng, Zhuochang Chen, Luoxian Zhang
This study aimed to validate whether transient receptor potential channel1 (TRPC1) and TRPC3 participate in the regulation the proliferation of airway smooth muscle cells (ASMCs) through modulating calcium ion (Ca2+ ) influx in vitro. Chronic model of murine asthma was induced and ASMCs isolated from asthmatic mice were used in this whole study. TRPC1 and TRPC3 were upregulated in asthmatic mouse ASMCs and selected for further investigation. Ca2+ concentration and the cell viability of asthmatic mouse ASMCs were significantly higher than that from non- asthma mice, however, TRPC channels blocker SKF96365 alleviated these effects...
March 25, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29561829/the-role-of-endothelial-ca-2-signaling-in-neurovascular-coupling-a-view-from-the-lumen
#5
REVIEW
Germano Guerra, Angela Lucariello, Angelica Perna, Laura Botta, Antonio De Luca, Francesco Moccia
BACKGROUND: Neurovascular coupling (NVC) is the mechanism whereby an increase in neuronal activity (NA) leads to local elevation in cerebral blood flow (CBF) to match the metabolic requirements of firing neurons. Following synaptic activity, an increase in neuronal and/or astrocyte Ca2+ concentration leads to the synthesis of multiple vasoactive messengers. Curiously, the role of endothelial Ca2+ signaling in NVC has been rather neglected, although endothelial cells are known to control the vascular tone in a Ca2+ -dependent manner throughout peripheral vasculature...
March 21, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29556099/an-optically-controlled-probe-identifies-lipid-gating-fenestrations-within-the-trpc3-channel
#6
Michaela Lichtenegger, Oleksandra Tiapko, Barbora Svobodova, Thomas Stockner, Toma N Glasnov, Wolfgang Schreibmayer, Dieter Platzer, Gema Guedes de la Cruz, Sarah Krenn, Romana Schober, Niroj Shrestha, Rainer Schindl, Christoph Romanin, Klaus Groschner
Transient receptor potential canonical (TRPC) channels TRPC3, TRPC6 and TRPC7 are able to sense the lipid messenger diacylglycerol (DAG). The DAG-sensing and lipid-gating processes in these ion channels are still unknown. To gain insights into the lipid-sensing principle, we generated a DAG photoswitch, OptoDArG, that enabled efficient control of TRPC3 by light. A structure-guided mutagenesis screen of the TRPC3 pore domain unveiled a single glycine residue behind the selectivity filter (G652) that is exposed to lipid through a subunit-joining fenestration...
March 19, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29522264/a-larixol-congener-with-high-affinity-and-subtype-selectivity-towards-trpc6
#7
Stephanie Häfner, Finn Burg, Martina Kannler, Nicole Urban, Peter Mayer, Alexander Dietrich, Dirk Trauner, Johannes Broichhagen, Michael Schaefer
Natural products have many health benefits and their application can improve the quality of life. Recently, the diterpene (+) larixol and its acetylated congeners demonstrated a selective inhibition of the second messenger-gated cation channel transient receptor potential canonical 6 (TRPC6) over its close isoforms TRPC3 and TRPC7. Building on this knowledge, we expand these findings by chemical diversification of (+)-larixol mostly at position C6. Implementing High-Throughput Ca2+ FLIPR screening assays and electrophysiological patch-clamp recordings, we showcase larixyl N-methylcarbamate, dubbed SH045, as a compound with nanomolar affinity and a 13-fold subtype selectivity over TRPC3 in stably expressing HEK293 cells...
March 9, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29449621/polygonum-aviculare-l-extract-and-quercetin-attenuate-contraction-in-airway-smooth-muscle
#8
Xi Luo, Lu Xue, Hao Xu, Qing-Yang Zhao, Qian Wang, Yu-Shan She, Dun-An Zang, Jinhua Shen, Yong-Bo Peng, Ping Zhao, Meng-Fei Yu, Weiwei Chen, Li-Qun Ma, Shu Chen, Shanshan Chen, Xiangning Fu, Sheng Hu, Xiaowei Nie, Chenyou Shen, Chunbin Zou, Gangjian Qin, Jiapei Dai, Guangju Ji, Yunchao Su, Shen Hu, Jingyu Chen, Qing-Hua Liu
Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+ -induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH)...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29438802/endocannabinoid-signaling-in-embryonic-neuronal-motility-and-cell-cell-contact-role-of-mglur5-and-trpc3-channels
#9
Pauli M Turunen, Lauri M Louhivuori, Verna Louhivuori, Jyrki P Kukkonen, Karl E Åkerman
Cell-cell communication plays a central role in the guidance of migrating neuronal precursor cells during the development of the cerebral cortex. Endocannabinoids (eCBs) have previously been shown to be one of the central factors regulating neuronal migration. In this study the effects of eCBs on different parameters, expected to affect embryonic cortical neuronal motility have been analyzed in neurosphere derived neuroblasts using time-lapse microscopy. Increased endogenous production of the endocannabinoid 2-arachidonyl glycerol (2-AG) causes bursts of neuroblast motility...
February 10, 2018: Neuroscience
https://www.readbyqxmd.com/read/29436640/-corrigendum-roles-and-mechanisms-of-trpc3-and-the-plc%C3%AE-pkc-cpi-17-signaling-pathway-in-regulating-parturition
#10
Jing Chen, Dongming Zheng, Hong Cui, Sishi Liu, Lijuan Zhang, Caixia Liu
Subsequent to the publication of the above paper, the authors have realized that there were errors in Fig. 1 on page 902; essentially, the experimental data described as being 'Preterm' and 'Infected preterm' should have been labelled as 'Full‑term without labor' and 'Preterm', respectively. Note that these errors in Fig. 1 were not reflected in the published figure legend. The corrected version of Fig. 1 is shown here. The authors sincerely apologize for this mistake, and regret any inconvenience this mistake has caused...
February 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29435486/transient-receptor-potential-channels-trpm4-and-trpc3-critically-contribute-to-respiratory-motor-pattern-formation-but-not-rhythmogenesis-in-rodent-brainstem-circuits
#11
Hidehiko Koizumi, Tibin T John, Justine X Chia, Mohammad F Tariq, Ryan S Phillips, Bryan Mosher, Yonghua Chen, Ryan Thompson, Ruli Zhang, Naohiro Koshiya, Jeffrey C Smith
Transient receptor potential channel, TRPM4, the putative molecular substrate for Ca2+-activated nonselective cation current (ICAN), is hypothesized to generate bursting activity of pre-Bötzinger complex (pre-BötC) inspiratory neurons and critically contribute to respiratory rhythmogenesis. Another TRP channel, TRPC3, which mediates Na+/Ca2+ fluxes, may be involved in regulating Ca2+-related signaling, including affecting TRPM4/ICAN in respiratory pre-BötC neurons. However, TRPM4 and TRPC3 expression in pre-BötC inspiratory neurons and functional roles of these channels remain to be determined...
January 2018: ENeuro
https://www.readbyqxmd.com/read/29402774/trpc3-overexpression-promotes-the-progression-of-inflammation-induced-preterm-labor-and-inhibits-t-cell-activation
#12
Chen Jing, Zheng Dongming, Cui Hong, Na Quan, Liu Sishi, Liu Caixia
BACKGROUND/AIMS: To detect the expression of the TRPC3 channel protein in the tissues of women experiencing preterm labor and investigate its interaction with T lymphocytes, providing a theoretical basis for the clinical prevention of threatened preterm labor and the development of drug-targeted therapy. METHODS: Forty-seven women experiencing preterm labor and 47 women experiencing normal full-term labor were included in this study. All included women underwent delivery via cesarean section; uterine samples were obtained at delivery...
January 22, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29392682/trpc3-is-required-for-the-survival-pluripotency-and-neural-differentiation-of-mouse-embryonic-stem-cells-mescs
#13
Helen Baixia Hao, Sarah E Webb, Jianbo Yue, Marc Moreau, Catherine Leclerc, Andrew L Miller
Transient receptor potential canonical subfamily member 3 (TRPC3) is known to be important for neural development and the formation of neuronal networks. Here, we investigated the role of TRPC3 in undifferentiated mouse embryonic stem cells (mESCs) and during the differentiation of mESCs into neurons. CRISPR/Cas9-mediated knockout (KO) of TRPC3 induced apoptosis and the disruption of mitochondrial membrane potential both in undifferentiated mESCs and in those undergoing neural differentiation. In addition, TRPC3 KO impaired the pluripotency of mESCs...
January 31, 2018: Science China. Life Sciences
https://www.readbyqxmd.com/read/29387339/mir-26a-inhibits-atherosclerosis-progression-by-targeting-trpc3
#14
Min Feng, Daqian Xu, Lirui Wang
Background: Atherosclerosis, a chronic multi-factorial vascular disease, has become a predominant cause of a variety of cardiovascular disorders. miR-26a was previously reported to be involved in atherosclerosis progression. However, the underlying mechanism of miR-26a in atherosclerosis remains to be further explained. Methods: High-fat diet (HFD)-fed apolipoprotein E (apoE)-/- mice and oxidized low-density lipoprotein (ox-LDL)-stimulated human aortic endothelial cells (HAECs) were established as in vivo and in vitro models of atherosclerosis...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29290366/reduced-calcification-and-osteogenic-features-in-advanced-atherosclerotic-plaques-of-mice-with-macrophage-specific-loss-of-trpc3
#15
Prabhatchandra R Dube, Lakshmikanth L Chikkamenahalli, Lutz Birnbaumer, Guillermo Vazquez
BACKGROUND AND AIMS: Recent in vitro studies have showed that in macrophages, deletion of the non-selective Ca2+ -permeable channel TRPC3 impairs expression of the osteogenic protein BMP-2. The pathophysiological relevance of this effect in atherosclerotic plaque calcification remains to be determined. METHODS: We used Ldlr-/- mice with macrophage-specific loss of TRPC3 (MacTrpc3-/- /Ldlr-/- ) to examine the effect of macrophage Trpc3 on plaque calcification and osteogenic features in advanced atherosclerosis...
March 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29247460/identification-of-an-englerin-a-analogue-which-antagonizes-englerin-a-at-trpc1-4-5-channels
#16
Hussein N Rubaiy, Tobias Seitz, Sven Hahn, Axel Choidas, Peter Habenberger, Bert Klebl, Klaus Dinkel, Peter Nussbaumer, Herbert Waldmann, Mathias Christmann, David J Beech
BACKGROUND AND PURPOSE: (-)-Englerin A (EA) is a potent cytotoxic agent against renal carcinoma cells. It achieves its effects by activation of transient receptor potential canonical (TRPC)4/TRPC1 heteromeric channels. It is also an agonist at channels formed by the related protein, TRPC5. Here, we sought an EA analogue, which might enable a better understanding of these effects of EA. EXPERIMENTAL APPROACH: An EA analogue, A54, was synthesized by chemical elaboration of EA...
March 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29167062/mechanistic-insights-into-the-detection-of-free-fatty-and-bile-acids-by-ileal-glucagon-like-peptide-1-secreting-cells
#17
Deborah A Goldspink, Van B Lu, Lawrence J Billing, Pierre Larraufie, Gwen Tolhurst, Fiona M Gribble, Frank Reimann
OBJECTIVES: The aim of this study was to investigate the electrical properties of ileal Glucagon-like peptide 1 (GLP-1) secreting L-cells using murine organoid cultures and the electrophysiological and intracellular signaling pathways recruited following activation of the Gαq -coupled free fatty acid receptors FFA1 and Gαs -coupled bile acid receptors GPBAR1. METHODS: Experiments were performed using ileal organoids generated from mice transgenically expressing fluorescent reporters (Epac2-camps and GCaMP3) under control of the proglucagon promoter...
January 2018: Molecular Metabolism
https://www.readbyqxmd.com/read/29122954/pregnancy-adapted-uterine-artery-endothelial-cell-ca2-signaling-and-its-relationship-with-membrane-potential
#18
Roxanne E Alvarez, Derek S Boeldt, Bikash R Pattnaik, Hannah L Friedman, Ian M Bird
Pregnancy-derived uterine artery endothelial cells (P-UAEC) express P2Y2 receptors and at high cell density show sustained and synchronous [Ca2+]i burst responses in response to ATP Bursts in turn require coupling of transient receptor potential canonical type3 channel (TRPC3) and inositol 1,4,5-triphosphate receptor type 2 (IP3R2), which is upregulated in P-UAEC in a manner dependent on connexin 43 (Cx43) gap junctions. While there is no known direct interaction of TRPC3 with Cx43, early descriptions of TRPC3 function showed it may also be influenced by altered membrane potential ( V m )...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29115500/roles-and-mechanisms-of-trpc3-and-the-plc%C3%AE-pkc-cpi-17-signaling-pathway-in-regulating-parturition
#19
Jing Chen, Dongming Zheng, Hong Cui, Sishi Liu, Lijuan Zhang, Caixia Liu
The aim of the current study was to investigate the role of phospholipase C (PLC)γ/protein kinase C (PKC)/C‑kinase‑activated protein phosphatase‑1 (CPI‑17) signaling pathways in uterine smooth muscle during parturition. Samples of uterine tissue were collected from pregnant patients who underwent a caesarean section for preterm delivery, full‑term delivery with labor onset, full‑term delivery without labor onset, and from a non‑pregnant control group undergoing surgery for cervical intraepithelial neoplasia III...
January 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29066740/trpc3-is-dispensable-for-%C3%AE-alanine-triggered-acute-itch
#20
Peter Dong, Changxiong Guo, Shengxiang Huang, Minghong Ma, Qin Liu, Wenqin Luo
The detection of pruritic (itchy) stimuli is mediated by a variety of receptors and channels expressed by primary sensory neurons. The G protein-coupled receptor (GPCR) MRGPRD is selectively expressed by a subset of mouse non-peptidergic nociceptors and functions as the molecular receptor for the itch-inducing chemical β-alanine. However, the channels responsible for generating electrical signals downstream of MRGPRD remain unclear. Here, we found that a member of the canonical TRP channel family, TRPC3, is highly expressed in MRGPRD+ non-peptidergic nociceptors, raising the possibility of whether TRPC3 functions as a downstream channel in the MRGPRD signaling pathway...
October 24, 2017: Scientific Reports
keyword
keyword
60360
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"