keyword
MENU ▼
Read by QxMD icon Read
search

Complex

keyword
https://www.readbyqxmd.com/read/29166726/-interaction-between-trpc1-and-stim1-in-calcium-sensing-receptor-mediated-calcium-influx-and-nitric-oxide-production-in-human-umbilical-vein-endothelial-cells
#1
L M Wang, H Zhong, N Tang, L J Pang, C J Zhang, F He
Objective: To investigate the interaction of Ca(2+) protein TRPC1 and STIM1 in extracellular Ca(2+) -sensing receptor (CaR)-induced extracellular Ca(2+) influx and the production of nitric oxide (NO). Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and incubated with CaR agonist spermine (activating store-operates cation channels (SOC) and receptor-operated channels (ROC)), CaR negative allosteric modulator Calhex231 (blocking SOC, activating ROC) and ROC analogue TPA (activating ROC, blocking SOC), protein kinase C (PKC) inhibitor Ro31-8220, PKCs and PKCμ inhibitor Go6967(activate SOC, blocking ROC), respectively...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166717/-left-atrial-appendage-volume-is-a-valuable-predictor-of-atrial-fibrillation-recurrence-after-radiofrequency-catheter-ablation
#2
G A Zheng, C Y Lin, L Weng, J D Chen
Objective: To investigate the association between the left atrial appendage (LAA) volume and atrial fibrillation (AF) recurrence after radiofrequency catheter ablation. Methods: We prospectively enrolled sixty-two patients with AF (40 cases with paroxysmal AF, 22 cases with persistent AF) who successfully underwent a first AF catheter ablation and had performed contrast-enhanced cardiac computed tomography (CT) prior to the procedure to measure LAA volumes in our hospital from January 2012 to August 2015. Circumferential pulmonary vein isolation was performed under the guidance of three-dimension mapping system (CARTO system)...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166684/-implantation-of-a-modular-distal-femoral-replacement-with-compressive-osseointegration-as-a-salvage-procedure-in-a-complex-femoral-posttraumatic-setting
#3
Anna Janine Schreiner, Peter Marten de Zwart, Christian Arnscheidt, Ulrich Stöckle, Björn Gunnar Ochs
Background Large bone defects and losses play a crucial role in both tumour surgery and in complex primary and revision total knee replacement. The established options of cemented or uncemented long intramedullary stems are limited by large bone defects and are at risk from relatively high exposure to aseptic loosening. There is no general valid agreement on implant fixation of the distal femur. A further option is the cementless fixation method with compressive osseointegration, based on the so-called Wolff law of bone remodelling...
November 22, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29166667/flotillin-scaffold-activity-contributes-to-type-vii-secretion-system-assembly-in-staphylococcus-aureus
#4
Benjamin Mielich-Süss, Rabea M Wagner, Nicole Mietrach, Tobias Hertlein, Gabriella Marincola, Knut Ohlsen, Sebastian Geibel, Daniel Lopez
Scaffold proteins are ubiquitous chaperones that promote efficient interactions between partners of multi-enzymatic protein complexes; although they are well studied in eukaryotes, their role in prokaryotic systems is poorly understood. Bacterial membranes have functional membrane microdomains (FMM), a structure homologous to eukaryotic lipid rafts. Similar to their eukaryotic counterparts, bacterial FMM harbor a scaffold protein termed flotillin that is thought to promote interactions between proteins spatially confined to the FMM...
November 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29166659/sex-specific-influences-of-mtdna-mitotype-and-diet-on-mitochondrial-functions-and-physiological-traits-in-drosophila-melanogaster
#5
Wen C Aw, Michael R Garvin, Richard G Melvin, J William O Ballard
Here we determine the sex-specific influence of mtDNA type (mitotype) and diet on mitochondrial functions and physiology in two Drosophila melanogaster lines. In many species, males and females differ in aspects of their energy production. These sex-specific influences may be caused by differences in evolutionary history and physiological functions. We predicted the influence of mtDNA mutations should be stronger in males than females as a result of the organelle's maternal mode of inheritance in the majority of metazoans...
2017: PloS One
https://www.readbyqxmd.com/read/29166655/how-well-do-you-know-your-mutation-complex-effects-of-genetic-background-on-expressivity-complementation-and-ordering-of-allelic-effects
#6
Christopher H Chandler, Sudarshan Chari, Alycia Kowalski, Lin Choi, David Tack, Michael DeNieu, William Pitchers, Anne Sonnenschein, Leslie Marvin, Kristen Hummel, Christian Marier, Andrew Victory, Cody Porter, Anna Mammel, Julie Holms, Gayatri Sivaratnam, Ian Dworkin
For a given gene, different mutations influence organismal phenotypes to varying degrees. However, the expressivity of these variants not only depends on the DNA lesion associated with the mutation, but also on factors including the genetic background and rearing environment. The degree to which these factors influence related alleles, genes, or pathways similarly, and whether similar developmental mechanisms underlie variation in the expressivity of a single allele across conditions and among alleles is poorly understood...
November 22, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29166646/reconstruction-of-membrane-current-by-deconvolution-and-its-application-to-membrane-capacitance-measurements-in-cardiac-myocytes
#7
Matej Hoťka, Ivan Zahradník
Correct detection of membrane currents under whole-cell patch-clamp conditions is limited by the transfer function of a recording system. The low-pass output filter of a recording amplifier alters the time course of membrane current and causes errors in relevant descriptors. To solve these problems, we developed a practical procedure for reconstruction of the time course of membrane currents based on deconvolution of recorded currents in frequency domain. The procedure was tested on membrane capacitance estimates from current responses to step voltage pulses...
2017: PloS One
https://www.readbyqxmd.com/read/29166626/non-covalent-interactions-between-thio-caffeine-derivatives-and-water-soluble-porphyrin-in-ethanol-water-environment
#8
Agnieszka Lipke, Magdalena Makarska-Bialokoz, Arleta Sierakowska, Beata Jasiewicz
To determine the binding interactions and ability to form the non-covalent systems, the association process between 5,10,15,20-tetrakis[4-(trimethylammonio)phenyl]-21H,23H-porphine tetra-p-tosylate (H2TTMePP) and a series of five structurally diverse thio-caffeine analogues has been studied in ethanol and ethanol-water solutions, analyzing its absorption and steady-state fluorescence spectra. The porphyrin fluorescence lifetimes in the systems studied were established as well. During the titration with thio-caffeine compounds the slight bathochromic effect and considerable hypochromicity of the porphyrin Soret band maximum can be noted...
November 19, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29166621/lifestyle-transitions-and-adaptive-pathogenesis-of-pseudomonas-aeruginosa
#9
REVIEW
Martina Valentini, Diego Gonzalez, Despoina Ai Mavridou, Alain Filloux
Pseudomonas aeruginosa acute and chronic infections are of great concern to human health, especially in hospital settings. It is currently assumed that P. aeruginosa has two antagonistic pathogenic strategies that parallel two different lifestyles; free-living cells are predominantly cytotoxic and induce an acute inflammatory reaction, while biofilm-forming communities cause refractory chronic infections. Recent findings suggest that the planktonic-to-sessile transition is a complex, reversible and overall dynamic differentiation process...
November 18, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29166617/spatiotemporal-proteomic-profiling-of-huntington-s-disease-inclusions-reveals-widespread-loss-of-protein-function
#10
Fabian Hosp, Sara Gutiérrez-Ángel, Martin H Schaefer, Jürgen Cox, Felix Meissner, Mark S Hipp, F-Ulrich Hartl, Rüdiger Klein, Irina Dudanova, Matthias Mann
Aggregation of polyglutamine-expanded huntingtin exon 1 (HttEx1) in Huntington's disease (HD) proceeds from soluble oligomers to late-stage inclusions. The nature of the aggregates and how they lead to neuronal dysfunction is not well understood. We employed mass spectrometry (MS)-based quantitative proteomics to dissect spatiotemporal mechanisms of neurodegeneration using the R6/2 mouse model of HD. Extensive remodeling of the soluble brain proteome correlated with insoluble aggregate formation during disease progression...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166612/impediment-of-replication-forks-by-long-non-coding-rna-provokes-chromosomal-rearrangements-by-error-prone-restart
#11
Takaaki Watanabe, Michael Marotta, Ryusuke Suzuki, Scott J Diede, Stephen J Tapscott, Atsushi Niida, Xiongfong Chen, Lila Mouakkad, Anna Kondratova, Armando E Giuliano, Sandra Orsulic, Hisashi Tanaka
Naturally stalled replication forks are considered to cause structurally abnormal chromosomes in tumor cells. However, underlying mechanisms remain speculative, as capturing naturally stalled forks has been a challenge. Here, we captured naturally stalled forks in tumor cells and delineated molecular processes underlying the structural evolution of circular mini-chromosomes (double-minute chromosomes; DMs). Replication forks stalled on the DM by the co-directional collision with the transcription machinery for long non-coding RNA...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166607/kdm2b-regulates-somatic-reprogramming-through-variant-prc1-complex-dependent-function
#12
Zhiwei Zhou, Xuejie Yang, Jiangping He, Jing Liu, Fang Wu, Shengyong Yu, Yuting Liu, Runxia Lin, He Liu, Yuanbin Cui, Chunhua Zhou, Xiaoshan Wang, Jian Wu, Shangtao Cao, Lin Guo, Lihui Lin, Tao Wang, Xiaozhong Peng, Boqing Qiang, Andrew P Hutchins, Duanqing Pei, Jiekai Chen
Polycomb repressive complex 1 (PRC1) plays essential roles in cell-fate determination. Recent studies have found that the composition of mammalian PRC1 is particularly varied and complex; however, little is known about the functional consequences of these variant PRC1 complexes on cell-fate determination. Here, we show that Kdm2b promotes Oct4-induced somatic reprogramming through recruitment of a variant PRC1 complex (PRC1.1) to CpG islands (CGIs). Furthermore, we find that bone morphogenetic protein (BMP) represses Oct4/Kdm2b-induced somatic reprogramming selectively...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166595/positive-and-negative-regulation-of-the-master-metabolic-regulator-mtorc1-by-two-families-of-legionella-pneumophila-effectors
#13
Justin A De Leon, Jiazhang Qiu, Christopher J Nicolai, Jessica L Counihan, Kevin C Barry, Li Xu, Rosalie E Lawrence, Brian M Castellano, Roberto Zoncu, Daniel K Nomura, Zhao-Qing Luo, Russell E Vance
All pathogens must acquire nutrients from their hosts. The intracellular bacterial pathogen Legionella pneumophila, the etiological agent of Legionnaires' disease, requires host amino acids for growth within cells. The mechanistic target of rapamycin complex 1 (mTORC1) is an evolutionarily conserved master regulator of host amino acid metabolism. Here, we identify two families of translocated L. pneumophila effector proteins that exhibit opposing effects on mTORC1 activity. The Legionella glucosyltransferase (Lgt) effector family activates mTORC1, through inhibition of host translation, whereas the SidE/SdeABC (SidE) effector family acts as mTORC1 inhibitors...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166587/migrating-myeloid-cells-sense-temporal-dynamics-of-chemoattractant-concentrations
#14
Caren E Petrie Aronin, Yun M Zhao, Justine S Yoon, Nicole Y Morgan, Thorsten Prüstel, Ronald N Germain, Martin Meier-Schellersheim
Chemoattractant-mediated recruitment of hematopoietic cells to sites of pathogen growth or tissue damage is critical to host defense and organ homeostasis. Chemotaxis is typically considered to rely on spatial sensing, with cells following concentration gradients as long as these are present. Utilizing a microfluidic approach, we found that stable gradients of intermediate chemokines (CCL19 and CXCL12) failed to promote persistent directional migration of dendritic cells or neutrophils. Instead, rising chemokine concentrations were needed, implying that temporal sensing mechanisms controlled prolonged responses to these ligands...
November 21, 2017: Immunity
https://www.readbyqxmd.com/read/29166586/the-broad-spectrum-of-human-natural-killer-cell-diversity
#15
REVIEW
Aharon G Freud, Bethany L Mundy-Bosse, Jianhua Yu, Michael A Caligiuri
Natural killer (NK) cells provide protection against infectious pathogens and cancer. For decades it has been appreciated that two major NK cell subsets (CD56(bright) and CD56(dim)) exist in humans and have distinct anatomical localization patterns, phenotypes, and functions in immunity. In light of this traditional NK cell dichotomy, it is now clear that the spectrum of human NK cell diversity is much broader than originally appreciated as a result of variegated surface receptor, intracellular signaling molecule, and transcription factor expression; tissue-specific imprinting; and foreign antigen exposure...
November 21, 2017: Immunity
https://www.readbyqxmd.com/read/29166580/a-sweet-solution-glycolysis-dependent-treg-cell-migration
#16
Ronen Alon
mTORC2 is a metabolic regulatory complex activated by PI3K. In this issue of Immunity, Kishore et al. (2017) demonstrate a specialized role for this complex in the migration of regulatory T (Treg) cells to sites of inflammation rather than their differentiation and survival.
November 21, 2017: Immunity
https://www.readbyqxmd.com/read/29166560/analytical-challenges-and-recent-advances-in-mass-spectrometry-based-lipidomics
#17
Yepy Hardi Rustam, Gavin Edmund Reid
Although no internationally accepted consensus definition exists, lipids can be broadly defined as "fatty acids and their derivatives, and substances related biosynthetically or functionally to these compounds". Lipids play critical roles as (i) the major structural components of biological membranes, and as functional and regulatory components of membrane protein signaling, (ii) bioactive intra- and inter-cellular signaling molecules, and (iii) energy storage molecules for the maintenance of energy homeostasis...
November 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29166490/-guidelines-for-a-line-of-care-for-children-and-adolescents-with-complex-chronic-health-conditions
#18
Martha Cristina Nunes Moreira, Lidianne Vianna Albernaz, Miriam Ribeiro Calheiros de Sá, Roberta Fernandes Correia, Roberta Falcão Tanabe
The definition of complex chronic health condition (CCHC) is important for identifying children and adolescents occupying pediatric beds in Brazilian hospitals, where they live out their childhood, requiring complex care by multidisciplinary teams in network format both in-hospital and in home care. The current article addresses guidelines for a line of care for children and adolescents with CCHCs. An expert panel validated 13 guidelines organized in three dimensions. Using the consensus conference technique, experts from different specialties validated the guidelines...
November 21, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/29166460/microsurgical-treatment-of-basilar-tip-aneurysms-is-it-still-acceptable
#19
Felix Hendrik Pahl, Matheus Fernandes de Oliveira, José Marcus Rotta
OBJECTIVE: Basilar tip aneurysms (BTAs) have a complex anatomy, making them difficult to treat. We describe our surgical results for BTAs. METHODS: From 2004 to 2015 (12 years), a total of 25 small BTAs and two giant BTAs were treated in the Hospital do Servidor Público Estadual de São Paulo. RESULTS: In 23 patients harboring aneurysms positioned anteriorly or straight, all aneurysms were clipped (complete exclusion in all on follow-up angiography)...
October 2017: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/29166416/limited-effect-of-cement-material-on-stress-distribution-of-a-monolithic-translucent-zirconia-crown-a-three-dimensional-finite-element-analysis
#20
Sebastian Franco-Tabares, Victoria Franke Stenport, Lars Hjalmarsson, Carina B Johansson
PURPOSE: The literature shows that the performance of densely sintered zirconia single crowns seems to be unaffected by the cement material. The purpose of this study was to evaluate the effects of various cement materials on the stress distribution in a monolithic translucent zirconia crown. MATERIALS AND METHODS: A crown-cement-dentin complex was modeled. Six cements were evaluated: zinc phosphate, glass-ionomer, resin-modified glass-ionomer, dual-cure resin, calcium aluminate-based, and a theoretical or conceptual cement with mechanical properties close to those of dentin (ie, monoblock cement)...
November 22, 2017: International Journal of Prosthodontics
keyword
keyword
6036
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"