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https://www.readbyqxmd.com/read/29471546/longitudinal-analyses-of-blood-transcriptome-during-conversion-to-psychosis
#1
Boris Chaumette, Oussama Kebir, Juliette Pouch, Bertrand Ducos, Fekrije Selimi, Raphael Gaillard, Marie-Odile Krebs
The biological processes associated with the onset of schizophrenia remain largely unknown. Current hypotheses favor gene × environment interactions as supported by our recent report about DNA methylation changes during the onset of psychosis. Here, we conducted the first longitudinal transcriptomic analysis of blood samples from 31 at-risk individuals who later converted to psychosis and 63 at-risk individuals who did not. Individuals were followed for a maximum of 1 year. Blood samples were collected at baseline and at the end of follow-up and individuals served as their own controls...
February 17, 2018: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/29471545/high-protein-distillers-dried-grains-with-solubles-produced-using-a-novel-front-end-back-end-fractionation-technology-has-greater-nutritional-value-than-conventional-distillers-dried-grains-with-solubles-when-fed-to-growing-pigs
#2
C D Espinosa, H H Stein
Two experiments were conducted to determine the standardized ileal digestibility (SID) of CP and AA, apparent total tract digestibility (ATTD) of GE, and DE and ME in conventional distillers dried grains with solubles (DDGS-CV) and in a novel source of high-protein distillers dried grains with solubles (DDGS-HP) produced by Lincolnway Energy (Nevada, IA). In Exp. 1, 18 barrows (initial BW: 72.47 ± 9.16 kg) that had a T-cannula installed in the distal ileum were allotted to a completely randomized design with 3 diets and 6 replicate pigs per diet...
February 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29471434/disease-definition-for-schizophrenia-by-functional-connectivity-using-radiomics-strategy
#3
Long-Biao Cui, Lin Liu, Hua-Ning Wang, Liu-Xian Wang, Fan Guo, Yi-Bin Xi, Ting-Ting Liu, Chen Li, Ping Tian, Kang Liu, Wen-Jun Wu, Yi-Huan Chen, Wei Qin, Hong Yin
Specific biomarker reflecting neurobiological substrates of schizophrenia (SZ) is required for its diagnosis and treatment selection of SZ. Evidence from neuroimaging has implicated disrupted functional connectivity in the pathophysiology. We aimed to develop and validate a method of disease definition for SZ by resting-state functional connectivity using radiomics strategy. This study included 2 data sets collected with different scanners. A total of 108 first-episode SZ patients and 121 healthy controls (HCs) participated in the current study, among which 80% patients and HCs (n = 183) and 20% (n = 46) were selected for training and testing in intra-data set validation and 1 of the 2 data sets was selected for training and the other for testing in inter-data set validation, respectively...
February 17, 2018: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/29471430/gwas-identifies-two-novel-colorectal-cancer-loci-at-16q24-1-and-20q13-12
#4
Chizu Tanikawa, Yoichiro Kamatani, Atsushi Takahashi, Yukihide Momozawa, Karine Leveque, Satoshi Nagayama, Koshi Mimori, Masaki Mori, Hideshi Ishii, Johji Inazawa, Jun Yasuda, Akito Tsuboi, Atsushi Shimizu, Makoto Sasaki, Taiki Yamaji, Norie Sawada, Motoki Iwasaki, Shoichiro Tsugane, Mariko Naito, Kenji Wakai, Teruhide Koyama, Toshiro Takezaki, Koichiro Yuji, Yoshinori Murakami, Yusuke Nakamura, Michiaki Kubo, Koichi Matsuda
Colorectal cancer is the fourth leading cause of cancer mortality worldwide. Genome-wide association studies (GWAS) identified more than 50 colorectal cancer loci. However, most of the previous studies were conducted in European populations, and host genetic factors among Japanese population are largely remained to be identified. To identify novel loci in the Japanese population, here, we performed a large-scale GWAS using 6,692 cases and 27,178 controls followed by a replication analysis using more than 11,000 case-control samples...
February 17, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29471393/the-severe-impairment-profile-a-conceptual-shift
#5
Jordan S Robinson, Robert L Collins, Brian I Miller, Vitor H Pacheco, Nicholas M Wisdom
Objective: The current study sought to evaluate and replicate the severe impairment profile (SIP) of the Word Memory Test (WMT) in patients referred for dementia evaluations. Method: The sample consisted of 125 patients referred for a neuropsychological evaluation at a large Veterans Affairs Medical Center. Patients were assigned a Clinical Dementia Rating (CDR) by blind raters, and were classified according to their performance on performance validity testing. Subsequent chart reviews were conducted to help in more accurately determining the presence of severe memory impairment likely due to an underlying dementing process versus poor effort/task engagement...
March 1, 2018: Archives of Clinical Neuropsychology: the Official Journal of the National Academy of Neuropsychologists
https://www.readbyqxmd.com/read/29471391/comparative-efficacy-of-enrofloxacin-to-that-of-tulathromycin-for-the-control-of-bovine-respiratory-disease-and-prevalence-of-antimicrobial-resistance-in-mannheimia-haemolytica-in-calves-at-high-risk-of-developing-bovine-respiratory-disease
#6
S Crosby, B Credille, S Giguère, R Berghaus
Bovine respiratory disease (BRD) is the most common cause of morbidity and mortality in North American beef cattle. Mannheimia haemolytica (Mh) is the bacterial pathogen most often isolated from cattle with BRD and the prevalence of antimicrobial resistance (AMR) in this organism has increased in recent years. Antimicrobials are commonly used to prevent BRD in cattle at high-risk of developing BRD, however, recent work would suggest that this practice might be one factor contributing to the increased prevalence of AMR in Mh...
February 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29471350/structural-and-mechanistic-insights-into-uhrf1-mediated-dnmt1-activation-in-the-maintenance-dna-methylation
#7
Tao Li, Linsheng Wang, Yongming Du, Si Xie, Xi Yang, Fuming Lian, Zhongjun Zhou, Chengmin Qian
UHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto replication foci. Here, we present the crystal structure of DNMT1 RFTS domain in complex with ubiquitin and highlight a unique ubiquitin binding mode for the RFTS domain. We provide evidence that UHRF1 N-terminal ubiquitin-like domain (UBL) also binds directly to DNMT1...
February 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471331/cross-cultural-validation-and-extension-of-the-clinical-assessment-interview-for-negative-symptoms-cains-in-the-chinese-context-evidence-from-a-spectrum-perspective
#8
Dong-Jie Xie, Hai-Song Shi, Simon S Y Lui, Chuan Shi, Ying Li, Karen K Y Ho, Karen S Y Hung, Wen-Xiu Li, Zheng-Hui Yi, Eric F C Cheung, Ann M Kring, Raymond C K Chan
The Clinical Assessment Interview for Negative Symptoms (CAINS) was designed in accordance with the recent theory and research in social affective neuroscience and to address the psychometric and conceptual limitations of other instruments assessing negative symptoms. The present study aimed to provide a large-scale validation of the CAINS in China and examine its applicability and validity evidence across the schizophrenia spectrum. Using confirmatory factor analysis, our results replicated the original findings in the US development samples that the CAINS possesses a stable 2-factor structure, namely "motivation/pleasure" and "expression"...
February 20, 2018: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/29471092/purification-and-functional-characterization-of-recombinant-balsamin-a-ribosome-inactivating-protein-from-momordica-balsamina
#9
Parminder K Ajji, Shailendra P Sonkar, Ken Walder, Munish Puri
Balsamin, a type I ribosome-inactivating protein (RIP), has been shown to inhibit HIV-1 replication at the translation step. Our recent studies have shown that balsamin also possess anti-tumor, antibacterial and DNase-like activity, however, the amount of natural balsamin in Momordica balsamina seeds is limited and preclinical studies require large quantities of pure, bioactive balsamin. Therefore, in this study, we cloned the balsamin gene, expressed it in E. coli BL21(DE3) strain and purified it by nickel affinity chromatography...
February 19, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29471085/houttuynia-cordata-polysaccharides-ameliorate-pneumonia-severity-and-intestinal-injury-in-mice-with-influenza-virus-infection
#10
Haiyan Zhu, Xiaoxiao Lu, Lijun Ling, Hong Li, Yingye Ou, Xunlong Shi, Yan Lu, Yunyi Zhang, Daofeng Chen
ETHNOPHARMACOLOGICAL RELEVANCE: Hottuynia cordata is an important traditional Chinese medicine for the treatment of respiratory diseases including bacterial and viral infections. Polysaccharides isolated from Houttuynia cordata (HCP), as its main ingredients, have been demonstrated to ameliorate the LPS-induced acute lung injury in mice. The study aimed to determine the protective effects of HCP on multiple organ injury in influenza A virus (IAV) H1N1 infected mice and its primary mechanisms in anti-inflammation and immune regulation...
February 19, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29471051/an-analysis-of-two-open-reading-frames-orf3-and-orf4-of-rat-hepatitis-e-virus-genome-using-its-infectious-cdna-clones-with-mutations-in-orf3-or-orf4
#11
Tanggis, Tominari Kobayashi, Masaharu Takahashi, Suljid Jirintai, Tsutomu Nishizawa, Shigeo Nagashima, Takashi Nishiyama, Satoshi Kunita, Emiko Hayama, Takeshi Tanaka, Mulyanto, Hiroaki Okamoto
Rat hepatitis E virus (ratHEV) genome has four open reading frames (ORFs: ORF1, ORF2, ORF3 and ORF4). The functions of ORF3 and ORF4 are unknown. An infectious cDNA clone (pUC-ratELOMB-131L_wt, wt) and its derivatives including ORF3-defective (ΔORF3) and ORF4-defective (ΔORF4) mutants, were constructed and their full-length RNA transcripts transfected into PLC/PRF/5 cells. ΔORF3 replicated as efficiently as wt in cells. However, ≤1/1,000 of the number of progenies were detectable in the culture supernatant of ΔORF3-infected cells compared with wt-infected cells...
February 19, 2018: Virus Research
https://www.readbyqxmd.com/read/29471016/sensitivity-to-psychosocial-chronic-stressors-and-adolescents-externalizing-problems-combined-moderator-effects-of-resting-heart-rate-and-parental-psychiatric-history
#12
Anna Roos E Zandstra, Johan Ormel, Andrea Dietrich, Edwin R van den Heuvel, Pieter J Hoekstra, Catharina A Hartman
From the literature it is not clear whether low resting heart rate (HR) reflects low or high sensitivity to the detrimental effects of adverse environments on externalizing problems. We studied parental psychiatric history (PH), reflecting general vulnerability, as possible moderator explaining these inconsistencies. Using Linear Mixed Models, we analyzed data from 1914 subjects, obtained in three measurement waves (mean age 11, 13.5, and 16 years) from the TRacking Adolescents' Individual Lives Survey population-based cohort and the parallel clinic-referred cohort...
February 19, 2018: Biological Psychology
https://www.readbyqxmd.com/read/29470991/modeling-factors-that-regulate-cell-cooperativity-in-the-zebrafish-posterior-lateral-line-primordium
#13
Leif Zinn-Björkman, Frederick R Adler
Collective cell migration is an integral part of organismal development. We consider migration of the zebrafish primordium during development of the posterior lateral line, a sensory system that detects water movement patterns. Experiments have shown that the chemokine ligand CXCL12a and its receptors CXCR4b and CXCR7b are key players for driving migration of the primordium, while FGF signaling helps maintain cohesion. In this work, we formulate a mathematical model of a laser ablated primordium separated into two smaller cell collectives: a leading collective that responds to local CXCL12a levels and a trailing collective that migrates up a local FGF gradient...
February 19, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29470858/genes-affecting-progression-of-bacteriophage-p22-infection-in-salmonella-identified-by-transposon-and-single-gene-deletion-screens
#14
Kaitlynne Bohm, Steffen Porwollik, Weiping Chu, John A Dover, Eddie B Gilcrease, Sherwood R Casjens, Michael McClelland, Kristin N Parent
Bacteriophages rely on their hosts for replication, and many host genes critically determine either viral progeny production or host success via phage resistance. A random insertion transposon library of 240,000 mutants in Salmonella enterica serovar Typhimurium was used to monitor effects of individual bacterial gene disruptions on bacteriophage P22 lytic infection. These experiments revealed candidate host genes that alter the timing of phage P22 propagation. Using a False Discovery Rate of <0.1, mutations in 235 host genes either blocked or delayed progression of P22 lytic infection, including many genes for which this role was previously unknown...
February 22, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29470740/effect-of-supplementing-germinated-sprouts-of-pulses-on-performance-carcass-variables-immune-and-oxidative-stress-indicators-in-broiler-chickens-reared-during-tropical-summer-season
#15
S V Rama Rao, B Prakash, U Rajkumar, M V L N Raju, T Srilatha, E P K Reddy
An experiment was conducted to study the effects of supplementing sprouts of pulses on performance, carcass variables, immune responses, and anti-oxidant variables in broiler chicken (day 1 to 6 weeks of age) reared during summer season in tropical region. Sprouts of black gram (BG, Vigna mungo), green gram (GG, Vigna radiata), and wild gram (WG, Vigna trilobata) were produced by soaking the pulses in water for 16 h and incubating at 37 °C for 24 h. Total phenolic content in sprouts of WG, BG, and GG was 102, 96...
February 22, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29470608/bacterial-communities-inhabiting-the-sponge-biemna-fortis-sediment-and-water-in-marine-lakes-and-the-open-sea
#16
Daniel F R Cleary, Ana R M Polónia, Nicole J de Voogd
Marine lakes are small bodies of landlocked seawater that are isolated from the open sea and have been shown to house numerous rare and unique taxa. The environmental conditions of the lakes are also characterised by lower pH and salinity and higher temperatures than generally found in the open sea. In the present study, we used a 16S rRNA gene barcoded pyrosequencing approach and a predictive metagenomic approach (PICRUSt) to examine bacterial composition and function in three distinct biotopes (sediment, water and the sponge species Biemna fortis) in three habitats (two marine lakes and the open sea) of the Berau reef system, Indonesia...
February 22, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29470549/genome-wide-identification-and-analysis-of-a-to-i-rna-editing-events-in-bovine-by-transcriptome-sequencing
#17
Mohammad Reza Bakhtiarizadeh, Abdolreza Salehi, Rocío Melissa Rivera
RNA editing increases the diversity of the transcriptome and proteome. Adenosine-to-inosine (A-to-I) editing is the predominant type of RNA editing in mammals and it is catalyzed by the adenosine deaminases acting on RNA (ADARs) family. Here, we used a largescale computational analysis of transcriptomic data from brain, heart, colon, lung, spleen, kidney, testes, skeletal muscle and liver, from three adult animals in order to identify RNA editing sites in bovine. We developed a computational pipeline and used a rigorous strategy to identify novel editing sites from RNA-Seq data in the absence of corresponding DNA sequence information...
2018: PloS One
https://www.readbyqxmd.com/read/29470544/validation-of-the-what-matters-index-a-brief-patient-reported-index-that-guides-care-for-chronic-conditions-and-can-substitute-for-computer-generated-risk-models
#18
John H Wasson, Lynn Ho, Laura Soloway, L Gordon Moore
INTRODUCTION: Current health care delivery relies on complex, computer-generated risk models constructed from insurance claims and medical record data. However, these models produce inaccurate predictions of risk levels for individual patients, do not explicitly guide care, and undermine health management investments in many patients at lesser risk. Therefore, this study prospectively validates a concise patient-reported risk assessment that addresses these inadequacies of computer-generated risk models...
2018: PloS One
https://www.readbyqxmd.com/read/29470542/functional-fine-tuning-between-bacterial-dna-recombination-initiation-and-quality-control-systems
#19
Veronika Ferencziová, Gábor M Harami, Julianna B Németh, Tibor Vellai, Mihály Kovács
Homologous recombination (HR) is crucial for the error-free repair of DNA double-strand breaks (DSBs) and the restart of stalled replication. However, imprecise HR can lead to genome instability, highlighting the importance of HR quality control. After DSB formation, HR proceeds via DNA end resection and recombinase loading, whereas helicase-catalyzed disruption of a subset of subsequently formed DNA invasions is thought to be essential for maintaining HR accuracy via inhibiting illegitimate (non-allelic) recombination...
2018: PloS One
https://www.readbyqxmd.com/read/29470511/preliminary-analysis-of-proteome-alterations-in-non-aneurysmal-internal-mammary-artery-tissue-from-patients-with-abdominal-aortic-aneurysms
#20
Christina Lund Kidholm, Hans Christian Beck, Julie Bukh Madsen, Nikolai Bjødstrup Palstrøm, Jes Sanddal Lindholt, Lars Melholt Rasmussen
OBJECTIVE: The pathogenesis of abdominal aortic aneurysms (AAA) involves a disturbed balance of breakdown and buildup of arterial proteins. We envision that individuals with AAA carry generalized arterial protein alterations either because of effects of genetically or environmental AAA risk factors or because of compensatory changes due to signaling molecules released from the affected aneurysmal tissue. APPROACH: Protein extraction and quantitative proteome analysis by LC-MS/MS (liquid chromatography-mass spectrometry) was done on individual samples from the internal mammary artery from 11 individuals with AAA and 33 sex- and age-matched controls without AAA...
2018: PloS One
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