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Lizbeth Acuña, Patricia Sánchez, Luis Alberto Soler, Luisa Fernanda Alvis
Objective To describe the demographic and clinical manifestations of patients with chronic kidney disease (CKD), arterial hypertension, and/or diabetes mellitus, and to determine the association between the presence of these pathologies and the development of CKD. Methods Analytic and cross-sectional study. The information, with a cutoff date of 30 June 2013, comes from the integrated database of CKD and patients with hypertension and diabetes, which the Colombian payer entities provided to the national fund for high-cost diseases (Cuenta de Alto Costo)...
August 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
Yuntao Shi, Marcus J C Long, Masha M Rosenberg, Shican Li, Aimee Kobjack, Philip Lessans, Rory T Coffey, Lizbeth Hedstrom
Targeted protein degradation is a promising strategy for drug design and functional assessment. Several small molecule approaches have been developed that localize target proteins to ubiquitin ligases, inducing ubiquitination and subsequent degradation by the 26S proteasome. We discovered that the degradation of a target protein can also be induced by a recognition ligand linked to tert-butyl carbamate (Boc3)-protected arginine (B3A). Here we show that this process requires the proteasome, but does not involve ubiquitination of the target protein...
October 5, 2016: ACS Chemical Biology
Ángel Mérida-Ortega, César Hernández-Alcaraz, Raúl U Hernández-Ramírez, Angélica García-Martínez, Belem Trejo-Valdivia, Aarón Salinas-Rodríguez, Katherine Svensson, Mariano E Cebrián, Francisco Franco-Marina, Lizbeth López-Carrillo
OBJECTIVE: To evaluate if selected phthalate exposure and flavonoid intake interact on breast cancer (BC) risk. MATERIAL AND METHODS: Interviews and urine samples were obtained from 233 women with histologically confirmed BC and 221 healthy controls matched by age and place of residence, from various states of northern Mexico. Urinary metabolites concentrations of diethyl phthalate (DEP), butyl benzyl phthalate (BBzP) and dioctyl phthalate (DOP) were determined by solid-phase extraction coupled with high-performance liquid chromatography/isotope dilution/tandem mass spectrometry...
September 27, 2016: Environment International
Laura Bouza-Mora, Gaby Dolz, Antony Solórzano-Morales, Juan José Romero-Zuñiga, Lizbeth Salazar-Sánchez, Marcelo B Labruna, Daniel M Aguiar
This study focuses on the detection and identification of DNA and antibodies to Ehrlichia spp. in samples of blood bank donors in Costa Rica using molecular and serological techniques. Presence of Ehrlichia canis was determined in 10 (3.6%) out of 280 blood samples using polymerase chain reaction (PCR) targeting the ehrlichial dsb conserved gene. Analysis of the ehrlichial trp36 polymorphic gene in these 10 samples revealed substantial polymorphism among the E. canis genotypes, including divergent tandem repeat sequences...
September 23, 2016: Ticks and Tick-borne Diseases
Sandra Aguayo-Patrón, Lizbeth Beltrán-Sauceda, Ana María Calderón de la Barca
Genotyping of HLA-DQ2 and DQ8 haplotypes is important for diagnosis or for screening of early risk detection of celiac disease or type 1 diabetes. Usually, venous blood DNA extraction and expensive and time consuming amplification are used, that hinder population-wide studies. We assayed a friendly HLA-DQ2 and DQ8 genotyping procedure using a combination of DNA from dried blood spot (DBS) and duplex allele-specific qPCR amplification using SYBR Green. DNA was extracted using home-made buffers and compared to an extraction commercial kit...
November 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Susan A Holechek, Megan S McAfee, Lizbeth M Nieves, Vanessa P Guzman, Kavita Manhas, Timothy Fouts, Kenneth Bagley, Joseph N Blattman
In order for vaccines to induce efficacious immune responses against mucosally transmitted pathogens, such as HIV-1, activated lymphocytes must efficiently migrate to and enter targeted mucosal sites. We have previously shown that all-trans retinoic acid (ATRA) can be used as a vaccine adjuvant to enhance mucosal CD8(+) T cell responses during vaccination and improve protection against mucosal viral challenge. However, the ATRA formulation is incompatible with most recombinant vaccines, and the teratogenic potential of ATRA at high doses limits its usage in many clinical settings...
September 23, 2016: Vaccine
Samantha A Green, Jeishla L M Matos, Akiko Yagi, Ryan A Shenvi
A combination of cobalt and nickel catalytic cycles enables a highly branch-selective (Markovnikov) olefin hydroarylation. Radical cyclization and ring scission experiments are consistent with hydrogen atom transfer (HAT) generation of a carbon-centered radical that leads to engagement of a nickel cycle.
October 5, 2016: Journal of the American Chemical Society
Hernán Ceballos, Juan C Pérez, Orlando Joaqui Barandica, Jorge I Lenis, Nelson Morante, Fernando Calle, Lizbeth Pino, Clair H Hershey
Breeding cassava relies on several selection stages (single row trial-SRT; preliminary; advanced; and uniform yield trials-UYT). This study uses data from 14 years of evaluations. From more than 20,000 genotypes initially evaluated only 114 reached the last stage. The objective was to assess how the data at SRT could be used to predict the probabilities of genotypes reaching the UYT. Phenotypic data from each genotype at SRT was integrated into the selection index (SIN) used by the cassava breeding program...
2016: Frontiers in Plant Science
Masha M Rosenberg, Alfred G Redfield, Mary F Roberts, Lizbeth Hedstrom
Guanosine-5'-monophosphate reductase (GMPR) catalyzes the reduction of GMP to IMP and ammonia with concomitant oxidation of NADPH. Here we investigated the structure and dynamics of enzyme-bound substrates and cofactors by measuring (31)P relaxation rates over a large magnetic field range using high-resolution field cycling NMR relaxometry. Surprisingly, these experiments reveal differences in the low field relaxation profiles for the monophosphate of GMP compared to IMP in their respective NADP(+) complexes...
September 9, 2016: Journal of Biological Chemistry
Omar Paul Arias Gaguancela, Lizbeth Peña Zúñiga, Alexis Vela Arias, Dennis Halterman, Francisco Javier Flores, Ida Elisabeth Johansen, Aiming Wang, Yasuyuki Yamaji, Jeanmarie Verchot-Lubicz
The inositol requiring enzyme (IRE1) is an endoplasmic reticulum (ER) stress sensor. When activated, it splices the bZIP60 mRNA producing a truncated transcription factor that upregulates genes involved in the unfolded protein response (UPR). Bax inhibitor 1 (BI-1) is another ER stress sensor that regulates cell death in response to environmental assaults. The potyvirus 6K2 and potexvirus TGB3 proteins are known to reside in the ER, serving respectively as anchors for the viral replicase and movement protein complex...
August 31, 2016: Molecular Plant-microbe Interactions: MPMI
Lizbeth López-Carrillo, Brenda Gamboa-Loira, Wendy Becerra, César Hernández-Alcaraz, Raúl Ulises Hernández-Ramírez, A Jay Gandolfi, Francisco Franco-Marina, Mariano E Cebrián
INTRODUCTION: Concentrations of inorganic arsenic (iAs) metabolites in urine present intra- and interindividual variations, which are determined not only by the magnitude of exposure to iAs, but also by differences in genetic, environmental and dietary factors. OBJECTIVE: To evaluate whether differences in dietary intake of selected micronutrients are associated with the metabolism of iAs. METHODS: The intake of 21 micronutrients was estimated for 1027 women living in northern Mexico using a food frequency questionnaire...
August 23, 2016: Environmental Research
Rebecca H Blair, Abigail E Horn, Yogitha Pazhani, Lizbeth Grado, James A Goodrich, Jennifer F Kugel
High mobility group box protein 1 (HMGB1) is an architectural protein that facilitates the formation of protein-DNA assemblies involved in transcription, recombination, DNA repair, and chromatin remodeling. Important to its function is the ability of HMGB1 to bend DNA non-sequence specifically. HMGB1 contains two HMG boxes that bind and bend DNA (the A box and the B box) and a C-terminal acidic tail. We investigated how these domains contribute to DNA bending by HMGB1 using single-molecule fluorescence resonance energy transfer (FRET), which enabled us to resolve heterogeneous populations of bent and unbent DNA...
October 9, 2016: Journal of Molecular Biology
Cristina P Pineda-Belmontes, Raúl U Hernández-Ramírez, César Hernández-Alcaraz, Mariano E Cebrián, Lizbeth López-Carrillo
OBJECTIVE: To evaluate whether the presence of polymorphisms of peroxisome proliferator-activated receptor gamma PPARγ (Pro 1 2Ala) and PPARGC1B (Ala203Pro) modifies the association between the inorganic arsenic (iAs) methylation capacity and breast cancer (BC). MATERIALS AND METHODS: Mexican women were interviewed, and blood and urine samples were collected from them (cases/controls= 197/220). The concentration of urinary arsenic species and the polymorphisms of interest were determined by high-performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and polymerase chain reaction (PCR), respectively...
April 2016: Salud Pública de México
Yang Wei, Petr Kuzmič, Runhan Yu, Gyan Modi, Lizbeth Hedstrom
Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP). The enzyme is an emerging target for antimicrobial therapy. The small molecule inhibitor A110 has been identified as a potent and selective inhibitor of IMPDHs from a variety of pathogenic microorganisms. A recent X-ray crystallographic study reported that the inhibitor binds to the NAD(+) cofactor site and forms a ternary complex with IMP. Here we report a pre-steady-state stopped-flow kinetic investigation of IMPDH from Bacillus anthracis designed to assess the kinetic significance of the crystallographic results...
September 20, 2016: Biochemistry
Vivian Colón-López, Lizbeth M Toro-Mejías, Alexandra Conde-Toro, Michelle J Serra-Rivera, Tania M Martínez, Verónica Rodríguez, Ana M Ríos, Luis Berdiel, Héctor Villanueva
OBJECTIVE: The purpose of this study was to understand through a quantitative assessment, the views of HPV and HPV vaccination among parents of sons from a FQHC in PR. METHODS: A self-administered questionnaire was given to a convenience sample of 200 parents of sons 9-17 years old. RESULTS: Nearly 30% of the parents reported that their sons had initiated the HPV vaccine regimen. Health care provider recommendation was significantly associated with vaccine initiation...
2016: Journal of Health Care for the Poor and Underserved
Sean A Newmister, Claire M Gober, Stelamar Romminger, Fengan Yu, Ashootosh Tripathi, Lizbeth Lorena L Parra, Robert M Williams, Roberto G S Berlinck, Madeleine M Joullié, David H Sherman
Indole alkaloids are a diverse class of natural products known for their wide range of biological activities and complex chemical structures. Rarely observed in this class are indolic nitrones, such as avrainvillamide and waikialoid, which possess potent bioactivities. Herein the oxa gene cluster from the marine-derived fungus Penicillium oxalicum F30 is described along with the characterization of OxaD, a flavin-dependent oxidase that generates roquefortine L, a nitrone-bearing intermediate in the biosynthesis of oxaline...
September 7, 2016: Journal of the American Chemical Society
Shih-Yin Tsai, Ariana A Rodriguez, Somasish G Dastidar, Elizabeth Del Greco, Kaili Lia Carr, Joanna M Sitzmann, Emmeline C Academia, Christian Michael Viray, Lizbeth Leon Martinez, Brian Stephen Kaplowitz, Travis D Ashe, Albert R La Spada, Brian K Kennedy
Obesity is a major risk factor driving the global type II diabetes pandemic. However, the molecular factors linking obesity to disease remain to be elucidated. Gender differences are apparent in humans and are also observed in murine models. Here, we link these differences to expression of eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1), which, upon HFD feeding, becomes significantly reduced in the skeletal muscle and adipose tissue of male but not female mice. Strikingly, restoring 4E-BP1 expression in male mice protects them against HFD-induced obesity and insulin resistance...
August 16, 2016: Cell Reports
Elena Zambrano, Tonantzin Sosa-Larios, Lizbeth Calzada, Carlos A Ibáñez, Carmen A Mendoza-Rodríguez, Angélica Morales, Sumiko Morimoto
Maternal obesity (MO) is a deleterious condition that enhances susceptibility of adult offspring to metabolic diseases such as type 2 diabetes. The objective is to study the effect of MO on in vitro insulin secretion and pancreatic cellular population in offspring. We hypothesize that a harmful antenatal metabolic environment due to MO diminishes the basal glucose-responsive secretory function of pancreatic beta cells in offspring. Mothers were fed a control (C) or high-fat diet from weaning through pregnancy (120 days) and lactation...
October 2016: Journal of Endocrinology
Sofía Rincón-Gallardo Patiño, Lizbeth Tolentino-Mayo, Eric Alejandro Flores Monterrubio, Jennifer L Harris, Stefanie Vandevijvere, Juan A Rivera, Simón Barquera
BACKGROUND: Evidence supports that television food advertisements influence children's food preferences and their consumption. However, few studies have examined the extent and nature of food marketing to children in low and middle income countries. This study aims to assess the nutritional quality of foods and beverages advertised on Mexican TV, applying the Mexican, World Health Organization (WHO) European and United Kingdom (UKNPM) nutrient profile models, before the Mexican regulation on food marketing came into effect...
2016: BMC Public Health
Lizbeth de la Cruz, Erika I Puente, Arturo Reyes-Vaca, Isabel Arenas, Julieta Garduño, Jorge Bravo-Martínez, David E Garcia
Phosphatidylinositol-4,5-bisphosphate (PIP2) is a membrane phosphoinositide that regulates the activity of many ion channels. Influx of calcium primarily through voltage-gated calcium (CaV) channels promotes insulin secretion in pancreatic β-cells. However, whether CaV channels are regulated by PIP2, as is the case for some non-insulin-secreting cells, is unknown. The purpose of this study was to investigate whether CaV channels are regulated by PIP2 depletion in pancreatic β-cells through activation of a muscarinic pathway induced by oxotremorine methiodide (Oxo-M)...
October 1, 2016: American Journal of Physiology. Cell Physiology
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