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https://www.readbyqxmd.com/read/29329758/real-time-rt-pcr-a-necessary-tool-to-support-the-diagnosis-and-surveillance-of-rotavirus-in-mexico
#1
Sergio Isaac De La Cruz Hernández, Yazmin Anaya Molina, Fabián Gómez Santiago, Heidi Lizbeth Terán Vega, Elda Monroy Leyva, Héctor Méndez Pérez, Herlinda García Lozano
Rotavirus produces diarrhea in children under 5 years old. Most of those conventional methods such as polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR) have been used for rotavirus detection. However, these techniques need a multi-step process to get the results. In comparison with conventional methods, the real-time RT-PCR is a highly sensitive method, which allows getting the results in only one day. In this study a real-time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR...
December 14, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29297684/isolation-derivative-synthesis-and-structure-activity-relationships-of-antiparasitic-bromopyrrole-alkaloids-from-the-marine-sponge-tedania-brasiliensis
#2
Lizbeth L L Parra, Ariane F Bertonha, Ivan R M Severo, Anna C C Aguiar, Guilherme E de Souza, Glaucius Oliva, Rafael V C Guido, Nathalia Grazzia, Tábata R Costa, Danilo C Miguel, Fernanda R Gadelha, Antonio G Ferreira, Eduardo Hajdu, Daniel Romo, Roberto G S Berlinck
The isolation and identification of a series of new pseudoceratidine (1) derivatives from the sponge Tedania brasiliensis enabled the evaluation of their antiparasitic activity against Plasmodium falciparum, Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) infantum, and Trypanosoma cruzi, the causative agents of malaria, cutaneous leishmaniasis, visceral leishmaniasis, and Chagas disease, respectively. The new 3-debromopseudoceratidine (4), 20-debromopseudoceratidine (5), 4-bromopseudoceratidine (6), 19-bromopseudoceratidine (7), and 4,19-dibromopseudoceratidine (8) are reported...
January 3, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29275169/testosterone-induces-hyporesponsiveness-by-interfering-with-ip3-receptors-in-guinea-pig-airway-smooth-muscle
#3
Luis M Montaño, Edgar Flores-Soto, Jorge Reyes-García, Verónica Díaz-Hernández, Abril Carbajal-García, Elías Campuzano-González, G Lizbeth Ramírez-Salinas, Marco A Velasco-Velázquez, Bettina Sommer
Asthma symptoms have been associated with sex steroids. During childhood, this illness seems more frequent in boys than in girls and this tendency reverts in puberty when it is more severe in women. Testosterone (TES), at supraphysiological concentrations, relaxed pre-contracted airway smooth muscle, but its effects at physiological concentrations have not been thoroughly studied. We explored this possibility in guinea pig tracheal smooth muscle. In myocytes TES (10 nM) abolished carbachol (CCh)-induced intracellular Ca2+ concentration ([Ca2+]i) increment...
December 21, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29258922/flow-virometry-as-a-tool-to-study-viruses
#4
REVIEW
J Lizbeth Zamora Reyes, Hector C Aguilar
In the last few decades, flow cytometry has redefined the field of biology, exponentially enhancing our understanding of cells, immunology, and microbiology. Flow cytometry recently gave birth to flow virometry, a new way to detect, analyze, and characterize single viral particles. Detection of viruses by flow cytometry is possible due to improvements in current flow cytometers, calibration and tuning methods. We summarize the recent birth and novel uses of flow virometry and the progressive evolution of this tool to advance the field of virology...
December 16, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29258830/trichinella-spiralis-muscle-larvae-excretory-secretory-products-induce-changes-in-cytoskeletal-and-myogenic-transcription-factors-in-primary-myoblast-cultures
#5
Lizbeth Hernández-Ancheyta, María Del Rosario Salinas-Tobón, Juan Carlos Cifuentes-Goches, Javier Hernández-Sánchez
Trichinella spiralis infection in skeletal muscle culminates with nurse cell formation. The participation of excretory-secretory products of the muscle larvae (ML-ESP) has been implicated in this process through different studies performed in infected muscle and the muscle cell line C2C12. In this work, we developed primary myoblast cultures to analyze the changes induced by ML-ESP in muscle cells. Microarray analyses revealed expression changes in muscle cell differentiation, proliferation, cytoskeleton organization, cell motion, transcription, cell cycle, apoptosis and signaling pathways such as MAPK, Jak-STAT, Wnt and PI3K-Akt...
December 16, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/29249112/-disseminated-infection-by-m-tuberculosis-complex-in-patient-with-ifn-%C3%AE-receptor-1-complete-deficiency
#6
María Cecilia Martínez-Morales, Carolina Deswarte, Jessica Castañeda-Casimiro, Jacinta Bustamante, Lizbeth Blancas-Galicia, Selma Scheffler-Mendoza
BACKGROUND: Several mutations have been described leading to impaired immunity in the IL-12/IFN-γ axis and, they confer susceptibility to mycobacterial infections. One of the more serious clinical phenotypes is secondary to mutations at IFN-γ receptor 1 gene, characterized by an early onset and more severe disease. CLINICAL REPORT: We present a 3-month-old female patient with systemic M. tuberculosis complex who has a homozygous mutation, it affects the splicing site at IFNGR1 c...
October 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29206824/-approach-to-percutaneous-nephrolithotomy-comparison-of-the-procedure-in-a-one-shot-versus-the-sequential-with-metal-dilata
#7
Ismael Sedano-Portillo, Gastón Ochoa-León, Clotilde Fuentes-Orozco, Leire Irusteta-Jiménez, Luis Rodrigo Michel-Espinoza, Marcela Salazar-Parra, Lizbeth Cuesta-Márquez, Alejandro González-Ojeda
INTRODUCTION: Percutaneous nephrolithotomy is an efficient approach for treatment of different types of kidney stones. Various types of access techniques have been described like sequential dilatation and one-shot procedure. OBJECTIVE: To determine the differences in time of exposure to X-rays and hemoglobin levels between techniques. METHODS: Controlled clinical trial. Patients older than 18 years with complex/uncomplicated kidney stones, without urine infection were included...
November 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/29206819/mechanisms-of-inactivation-of-the-tumour-suppressor-gene-rhoa-in-colorectal-cancer
#8
Higinio Dopeso, Paulo Rodrigues, Josipa Bilic, Sarah Bazzocco, Fernando Cartón-García, Irati Macaya, Priscila Guimarães de Marcondes, Estefanía Anguita, Marc Masanas, Lizbeth M Jiménez-Flores, Águeda Martínez-Barriocanal, Rocío Nieto, Miguel F Segura, Simo Schwartz, John M Mariadason, Diego Arango
BACKGROUND: Reduced RHOA signalling has been shown to increase the growth/metastatic potential of colorectal tumours. However, the mechanisms of inactivation of RHOA signalling in colon cancer have not been characterised. METHODS: A panel of colorectal cancer cell lines and large cohorts of primary tumours were used to investigate the expression and activity of RHOA, as well as the presence of RHOA mutations/deletions and promoter methylation affecting RHOA. Changes in RHOA expression were assessed by western blotting and qPCR after modulation of microRNAs, SMAD4 and c-MYC...
December 5, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29194458/evidence-of-increasing-sedentarism-in-mexico-city-during-the-last-decade-sitting-time-prevalence-trends-and-associations-with-obesity-and-diabetes
#9
Catalina Medina, Lizbeth Tolentino-Mayo, Ruy López-Ridaura, Simón Barquera
INTRODUCTION: Sedentary behaviors such as sitting time are associated with obesity and diabetes independently of total reported physical activity. This study aimed to describe the current sitting time/day prevalence and trends and to examine the association of sitting time with sociodemographic and clinical variables in Mexico City. METHODS: Two cross-sectional representative surveys in Mexico City were used for this analysis (2006: n = 1148 and 2015: n = 1329)...
2017: PloS One
https://www.readbyqxmd.com/read/29170203/identification-of-a-novel-locus-on-chromosome-2q13-which-predisposes-to-clinical-vertebral-fractures-independently-of-bone-density
#10
Nerea Alonso, Karol Estrada, Omar M E Albagha, Lizbeth Herrera, Sjur Reppe, Ole K Olstad, Kaare M Gautvik, Niamh M Ryan, Kathryn L Evans, Carrie M Nielson, Yi-Hsiang Hsu, Douglas P Kiel, George Markozannes, Evangelia E Ntzani, Evangelos Evangelou, Bjarke Feenstra, Xueping Liu, Mads Melbye, Laura Masi, Maria Luisa Brandi, Philip Riches, Anna Daroszewska, José Manuel Olmos, Carmen Valero, Jesús Castillo, José A Riancho, Lise B Husted, Bente L Langdahl, Matthew A Brown, Emma L Duncan, Stephen Kaptoge, Kay-Tee Khaw, Ricardo Usategui-Martín, Javier Del Pino-Montes, Rogelio González-Sarmiento, Joshua R Lewis, Richard L Prince, Patrizia D'Amelio, Natalia García-Giralt, Xavier Nogués, Simona Mencej-Bedrac, Janja Marc, Orit Wolstein, John A Eisman, Ling Oei, Carolina Medina-Gómez, Katharina E Schraut, Pau Navarro, James F Wilson, Gail Davies, John Starr, Ian Deary, Toshiko Tanaka, Luigi Ferrucci, Fernando Gianfrancesco, Luigi Gennari, Gavin Lucas, Roberto Elosua, André G Uitterlinden, Fernando Rivadeneira, Stuart H Ralston
OBJECTIVES: To identify genetic determinants of susceptibility to clinical vertebral fractures, which is an important complication of osteoporosis. METHODS: Here we conduct a genome-wide association study in 1553 postmenopausal women with clinical vertebral fractures and 4340 controls, with a two-stage replication involving 1028 cases and 3762 controls. Potentially causal variants were identified using expression quantitative trait loci (eQTL) data from transiliac bone biopsies and bioinformatic studies...
November 23, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29162468/an-assessment-of-rehabilitation-infrastructure-in-peru
#11
Amy K Fuhs, Lacey N LaGrone, Miguel G Moscoso Porras, Manuel J A Rodríguez Castro, Rosa Lizbeth Ecos Quispe, Charles N Mock
OBJECTIVE: To assess rehabilitation infrastructure in Peru in terms of WHO Health System Building Blocks. DESIGN: Anonymous quantitative survey. Questions were based on WHO Guidelines for Essential Trauma Care and rehabilitation professionals' input. SETTING: Nine large public hospitals and referral centers in Lima and an online survey platform. PARTICIPANTS: Convenience sample of hospital personnel working in rehabilitation and neurology, recruited through existing contacts and professional societies...
November 18, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29161480/-biological-parameters-of-meccus-phyllosomus-phyllosomus-burmeister-1835-triatoma-recurva-st%C3%A3-l-1868-hemiptera-reduviidae-and-their-laboratory-hybrids
#12
Lizbeth Díaz, Karen Covarrubias, Ángel Licón, Mixtli Astorga, Yaneth Moreno, José Alejandro Martínez
INTRODUCTION: Hybrids from species and subspecies of Mexican triatomines have shown outstanding biological and behavioral characteristics as compared with their parental groups. This could lead to an increasing risk of transmission of Trypanosoma cruzi to human and animal reservoir hosts. OBJECTIVE: To describe and compare biological parameters related to the life cycle of Triatoma recurva, Meccus phyllosomus phyllosomus and their laboratory hybrids and estimate their risk as transmitters of T...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29154747/disseminated-methicillin-susceptible-staphylococcus-aureus-infection
#13
David R Perkins, James A Hall, Lisa M Lopez, Lizbeth Janice Cahuayme-Zuniga
INTRODUCTION: When clinicians think about Staphylococcus aureus bacteria, what comes to the mind of most is the dreaded methicillin-resistant form. However, clinicians should not forget the methicillin-susceptible type, which is just as virulent. CASE PRESENTATION: The authors present the case of a 20-year-old woman who was admitted with septic shock and multi-organ failure and was found to have disseminated methicillin-susceptible Staphylococcus aureus (MSSA) infection...
November 20, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29097064/acanthamoeba-t4-trophozoites-cross-the-mdck-epithelium-without-cell-damage-but-increase-paracellular-permeability-and-transepithelial-resistance-by-modifying-tight-junction-composition
#14
Catalina Flores-Maldonado, Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Juan Manuel Gallardo, Mónica González-Lázaro, Verónica Ivonne Hernández-Ramírez, Patricia Talamás-Rohana, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
Free-living amoebae of the genus Acanthamoeba are protozoa ubiquitously found in nature. Some species of the genus are potentially pathogenic for humans provoking keratitis in healthy individuals, often in contact lens wearers and opportunistic infections such as pneumonitis, fatal granulomatous encephalitis and skin infections, particularly in immunocompromised individuals. The pathogenic mechanisms of these amoebae are poorly understood, however it had been suggested that contact dependent mechanisms are important during invasion, regardless of the epithelia type, since amoebae penetrate epithelia separating tight junction (TJ)...
October 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29081270/home-based-telehealth-service-for-swallowing-and-nutrition-management-following-head-and-neck-cancer-treatment
#15
Annette Collins, Clare L Burns, Elizabeth C Ward, Tracy Comans, Claire Blake, Lizbeth Kenny, Phil Greenup, Daniel Best
Introduction Following (chemo)radiotherapy (C/RT) for head and neck cancer (HNC), patients return to hospital for regular outpatient reviews with speech pathology (SP) and nutrition and dietetics (ND) for acute symptom monitoring, nutritional management, and swallowing and communication rehabilitation. The aim of the current study was to determine the feasibility of a home-based telehealth model for delivering SP and ND reviews, to provide patients with more convenient access to these appointments. Methods Service outcomes, costs, and consumer satisfaction were examined across 30 matched participants: 15 supported via the standard model of care (SMOC), and 15 via the home-based telehealth model of care (TMOC)...
December 2017: Journal of Telemedicine and Telecare
https://www.readbyqxmd.com/read/29067766/molprobity-more-and-better-reference-data-for-improved-all-atom-structure-validation
#16
Christopher J Williams, Jeffrey J Headd, Nigel W Moriarty, Michael G Prisant, Lizbeth L Videau, Lindsay N Deis, Vishal Verma, Daniel A Keedy, Bradley J Hintze, Vincent B Chen, Swati Jain, Steven M Lewis, W Bryan Arendall, Jack Snoeyink, Paul D Adams, Simon C Lovell, Jane S Richardson, David C Richardson
This paper describes the current update on macromolecular model validation services that are provided at the MolProbity website, emphasizing changes and additions since the previous review in 2010. There have been many infrastructure improvements, including rewrite of previous Java utilities to now use existing or newly written Python utilities in the open-source CCTBX portion of the Phenix software system. This improves long-term maintainability and enhances the thorough integration of MolProbity-style validation within Phenix...
October 25, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29063444/fast-inactivation-of-cav2-2-channels-is-prevented-by-the-g%C3%AE-1-subunit-in-rat-sympathetic-neurons
#17
Arturo Reyes-Vaca, Lizbeth de la Cruz, Julieta Garduño, Isabel Arenas, David E Garcia
Voltage-dependent regulation of CaV2.2 channels by G-proteins is performed by the β (Gβ) subunit. Most studies of regulation by G-proteins have focused on channel activation; however, little is known regarding channel inactivation. This study investigated inactivation of CaV2.2 channels in superior cervical ganglion neurons that overexpressed Gβ subunits. CaV2.2 currents were recorded by whole-cell patch clamping configuration. We found that the Gβ1 subunit reduced inactivation, while Gβ5 subunit did not alter at all inactivation kinetics compared to control recordings...
October 23, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29050936/t%C3%AE-riii-is-induced-by-tcr-signaling-and-downregulated-in-foxp3-regulatory-t-cells
#18
Sandra Ortega-Francisco, Marisol de la Fuente-Granada, Evelyn K Alvarez Salazar, Lizbeth Airais Bolaños-Castro, Gabriela Fonseca-Camarillo, Roxana Olguin-Alor, Germán R Alemán-Muench, Fernando López-Casillas, Chander Raman, Eduardo A García-Zepeda, Gloria Soldevila
TGF-β type III receptor (TβRIII) is a co-receptor for TGFβ family members required for high-affinity binding of these ligands to their receptors, potentiating their cellular functions. TGF-βs, Bone Morphogenetic Proteins (BMP2/4) and Inhibins/Activins regulate different checkpoints during T cell differentiation. We have previously reported that TβRIII modulates T cell development by protecting developing thymocytes from apoptosis, however the role of this co-receptor in peripheral lymphocytes still remains elusive...
December 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29050783/design-and-synthesis-of-a-novel-series-of-1-s-2r-4-s-3h-4-azaspiro-benzo-4-5-imidazo-2-1-b-oxazole-2-2-bicyclo-2-2-2-octanes-with-high-affinity-for-the-%C3%AE-7-neuronal-nicotinic-receptor
#19
James Cook, F Christopher Zusi, Matthew D Hill, Haiquan Fang, Bradley Pearce, Hyunsoo Park, Lizbeth Gallagher, Ivar M McDonald, Linda Bristow, John E Macor, Richard E Olson
We describe an efficient and convergent synthesis of a series of (1'S,2R,4'S)-3H-4'-azaspiro[benzo[4,5]imidazo[2,1-b]oxazole-2,2'-bicyclo[2.2.2]octanes] displaying potency for the α7 nicotinic acetylcholine receptor (nAChR) and good selectivity vs. the related 5-HT3A receptor.
November 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29050703/validity-of-the-edinburgh-claudication-questionnaire-for-diagnosis-of-peripheral-artery-disease-in-patients-with-type-2-diabetes
#20
Salvador Pita-Fernández, María José Modroño-Freire, Sonia Pértega-Díaz, Lizbeth Herrera-Díaz, Teresa Seoane-Pillado, Adriana Paz-Solís, José Luis Varela Modroño
OBJECTIVE: To assess the prevalence of peripheral artery disease and the validity of clinical signs for its diagnosis in patients with type 2 diabetes. METHODS: Setting: Health center (Mariñamansa,Orense). PERIOD: January 2011-January 2013. INCLUSION CRITERIA: Patients with type 2 diabetes, informed consent. MEASUREMENTS: Age, sex, diabetes duration, body mass index, Charlson index, blood pressure, ankle-brachial index (ABI), cholesterol levels, smoking...
November 2017: Endocrinología, Diabetes y Nutrición
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