keyword
MENU ▼
Read by QxMD icon Read
search

Integrase

keyword
https://www.readbyqxmd.com/read/28535266/mechanism-of-bacterial-gene-rearrangement-spra-catalyzed-precise-dna-recombination-and-its-directionality-control-by-sprb-ensure-the-gene-rearrangement-and-stable-expression-of-spsm-during-sporulation-in-bacillus-subtilis
#1
Kimihiro Abe, Takuo Takamatsu, Tsutomu Sato
A sporulation-specific gene, spsM, is disrupted by an active prophage, SPβ, in the genome of Bacillus subtilis. SPβ excision is required for two critical steps: the onset of the phage lytic cycle and the reconstitution of the spsM-coding frame during sporulation. Our in vitro study demonstrated that SprA, a serine-type integrase, catalyzed integration and excision reactions between attP of SPβ and attB within spsM, while SprB, a recombination directionality factor, was necessary only for the excision between attL and attR in the SPβ lysogenic chromosome...
May 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28533248/impact-of-hiv-1-integrase-l74f-v75i-mutations-from-a-clinical-isolate-on-resistance-to-second-generation-integrase-strand-transfer-inhibitors
#2
Atsuko Hachiya, Karen A Kirby, Yoko Ido, Urara Shigemi, Masakazu Matsuda, Reiko Okazaki, Junji Imamura, Stefan G Sarafianos, Yoshiyuki Yokomaku, Yasumasa Iwatani
A novel HIV-1 integrase mutation pattern, L74F/V75I, which conferred resistance to first-generation integrase strand transfer inhibitors (INSTIs), was identified in a clinical case with virological failure under a raltegravir-based regimen. Addition of L74F/V75I to N155H or G140S/Q148H increased resistance levels to second-generation INSTIs, dolutegravir (>385-, 100-fold, respectively) and cabotegravir (153-, 197-fold, respectively). These findings are important for developing an accurate interpretation system of INSTI resistance and the rational design of next-generation INSTIs...
May 22, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28525596/antiretroviral-therapy-containing-hiv-protease-inhibitors-enhances-fracture-risk-by-impairing-osteoblast-differentiation-and-bone-quality
#3
Hiroyuki Hirakawa, Hiroyuki Gatanaga, Hiroki Ochi, Toru Fukuda, Satoko Sunamura, Shinichi Oka, Shu Takeda, Shingo Sato
Long-term ART is associated with increased fracture risk, but the mechanism remains elusive. We measured serum undercarboxylated osteocalcin and pentosidine (markers of poor bone quality) in HIV-infected patients treated with protease inhibitors (PIs) or an integrase strand transfer inhibitor (INSTI)-containing regimens. The results demonstrated significantly higher undercarboxylated osteocalcin and pentosidine in PIs-treated patients. Switching to INSTI resulted in significant decrease in these markers. We also showed impaired bone mechanical properties with higher undercarboxylated osteocalcin level in PIs-treated mice and inhibited osteoblast differentiation in PIs-treated osteogenic cells...
May 19, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28515479/acquisition-of-virulence-factors-in-livestock-associated-mrsa-lysogenic-conversion-of-cc398-strains-by-virulence-gene-containing-phages
#4
Britta Kraushaar, Jens Andre Hammerl, Marina Kienöl, Marie Luise Heinig, Nina Sperling, Mai Dinh Thanh, Jochen Reetz, Claudia Jäckel, Alexandra Fetsch, Stefan Hertwig
Staphylococcus aureus MRSA strains belonging to the clonal complex 398 (CC398) are highly prevalent in livestock and companion animals but may also cause serious infections in humans. CC398 strains in livestock usually do not possess well-known virulence factors that can be frequently found in other MRSA sequence types (ST). Since many staphylococcal virulence genes are residing on the genomes of temperate phages, the question arises why livestock-associated (LA-) CC398 strains are only rarely infected by those phages...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28503314/atypical-teratoid-rhabdoid-tumor-of-lumbar-spine-in-a-toddler-child
#5
Min Fang Chao, Yu-Feng Su, Twei-Shiun Jaw, Shyh-Shin Chiou, Chih-Hung Lin
BACKGROUND: Atypical teratoid/rhabdoid tumor (AT/RT) is a rare pediatric central nervous system malignancy with poor outcome. AT/RT is infrequently located in the spinal cord. CLINICAL PRESENTATION: A 16-month-old boy presented with progressive urinary retention and weakness of the lower extremities. Magnetic resonance imaging of the spine revealed an intradural extramedullary mass occupying the spinal canal at the level of T10-L3. The tumor was successfully resected by using neuroendoscopy...
2017: Spinal Cord Series and Cases
https://www.readbyqxmd.com/read/28498892/incident-aids-or-death-after-initiation-of-human-immunodeficiency-virus-treatment-regimens-including-raltegravir-or-efavirenz-among-adults-in-the-united-states
#6
Stephen R Cole, Jessie K Edwards, H Irene Hall, M Alan Brookhart, W Christopher Mathews, Richard D Moore, Heidi M Crane, Mari M Kitahata, Michael J Mugavero, Michael S Saag, Joseph J Eron
Background.: The long-term effectiveness of human immunodeficiency virus (HIV) treatments containing integrase inhibitors is unknown. Methods.: We use observational data from the Centers for AIDS Research Network of Integrated Clinical Systems and the Centers for Disease Control and Prevention to estimate 4-year risk of AIDS and all-cause mortality among 415 patients starting a raltegravir regimen compared to 2646 starting an efavirenz regimen (both regimens include emtricitabine and tenofovir disoproxil fumarate)...
June 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28497073/integrase-deficient-lentiviral-vector-as-an-all-in-one-platform-for-highly-efficient-crispr-cas9-mediated-gene-editing
#7
Pavel I Ortinski, Bernadette O'Donovan, Xiaoyu Dong, Boris Kantor
The CRISPR/Cas9 systems have revolutionized the field of genome editing by providing unprecedented control over gene sequences and gene expression in many species, including humans. Lentiviral vectors (LVs) are one of the primary delivery platforms for the CRISPR/Cas9 system due to their ability to accommodate large DNA payloads and sustain robust expression in a wide range of dividing and non-dividing cells. However, long-term expression of LV-delivered Cas9/guide RNA may lead to undesirable off-target effects characterized by non-specific RNA-DNA interactions and off-target DNA cleavages...
June 16, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28494325/hiv-1-integrase-inhibitor-resistance-among-treatment-na%C3%A3-ve-patients-in-the-west-of-scotland
#8
A Bradley-Stewart, C Urcia, A MacLean, C Aitken, R Gunson
BACKGROUND: Transmitted integrase inhibitor resistance is rare, with only a small number of cases reported world-wide to date. OBJECTIVES: The aim of this study was to assess whether transmitted integrase inhibitor resistance has occurred in Scotland and if so, could there be a case for performing genotypic integrase resistance testing at baseline. STUDY DESIGN: The study population consisted of 106 treatment naïve, newly diagnosed, HIV positive patients...
April 23, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28494151/design-of-hiv-co-receptor-derived-peptides-that-inhibit-viral-entry-at-submicromolar-concentrations
#9
Kostyantyn D Bobyk, Sivakoteswara R Mandadapu, Katheryn Lohith, Christina Guzzo, Abhishek Bhargava, Paolo Lusso, Carole A Bewley
HIV/AIDS continues to pose an enormous burden on global health. Current HIV therapeutics include inhibitors that target the enzymes HIV protease, reverse transcriptase and integrase, along with viral entry inhibitors that block the initial steps of HIV infection by preventing membrane fusion or virus-coreceptor interactions. With regard to the latter, peptides derived from the HIV coreceptor CCR5 were previously shown to modestly inhibit entry of CCR5-tropic HIV strains, with a peptide containing residues 178-191 of the second extracellular loop (peptide 2C) showing the strongest inhibition...
May 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28489061/microbial-natural-product-alternariol-5-o-methyl-ether-inhibits-hiv-1-integration-by-blocking-nuclear-import-of-the-pre-integration-complex
#10
Jiwei Ding, Jianyuan Zhao, Zhijun Yang, Ling Ma, Zeyun Mi, Yanbing Wu, Jiamei Guo, Jinmin Zhou, Xiaoyu Li, Ying Guo, Zonggen Peng, Tao Wei, Haisheng Yu, Liguo Zhang, Mei Ge, Shan Cen
While Highly Active Antiretroviral Therapy (HAART) has significantly decreased the mortality of human immunodeficiency virus (HIV)-infected patients, emerging drug resistance to approved HIV-1 integrase inhibitors highlights the need to develop new antivirals with novel mechanisms of action. In this study, we screened a library of microbial natural compounds from endophytic fungus Colletotrichum sp. and identified alternariol 5-O-methyl ether (AME) as a compound that inhibits HIV-1 pre-integration steps. Time-of addition analysis, quantitative real-time PCR, confocal microscopy, and WT viral replication assay were used to elucidate the mechanism...
May 10, 2017: Viruses
https://www.readbyqxmd.com/read/28485709/occurrence-and-characterization-of-class-1-integrons-in-escherichia-coli-from-healthy-individuals-and-those-with-urinary-infection
#11
Clarisse Oliveira-Pinto, Cristiane Diamantino, Patrícia L Oliveira, Mariana P Reis, Patrícia S Costa, Magna C Paiva, Regina M D Nardi, Paula P Magalhães, Edmar Chartone-Souza, Andréa M A Nascimento
PURPOSE: Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40). METHODOLOGY: The methods used included PCR, sequencing and antimicrobial susceptibility testing...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28472712/casposons-mobile-genetic-elements-that-gave-rise-to-the-crispr-cas-adaptation-machinery
#12
REVIEW
Mart Krupovic, Pierre Béguin, Eugene V Koonin
A casposon, a member of a distinct superfamily of archaeal and bacterial self-synthesizing transposons that employ a recombinase (casposase) homologous to the Cas1 endonuclease, appears to have given rise to the adaptation module of CRISPR-Cas systems as well as the CRISPR repeats themselves. Comparison of the mechanistic features of the reactions catalyzed by the casposase and the Cas1-Cas2 heterohexamer, the CRISPR integrase, reveals close similarity but also important differences that explain the requirement of Cas2 for integration of short DNA fragments, the CRISPR spacers...
May 1, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28472323/hiv-1-strains-belonging-to-large-phylogenetic-clusters-show-accelerated-escape-from-integrase-inhibitors-in-cell-culture-compared-with-viral-isolates-from-singleton-small-clusters
#13
Bluma G Brenner, Ruxandra-Ilinca Ibanescu, Maureen Oliveira, Michel Roger, Isabelle Hardy, Jean-Pierre Routy, Fred Kyeyune, Miguel E Quiñones-Mateu, Mark A Wainberg
Objectives: Viral phylogenetics revealed two patterns of HIV-1 spread among MSM in Quebec. While most HIV-1 strains ( n  =   2011) were associated with singleton/small clusters (cluster size 1-4), 30 viral lineages formed large networks (cluster size 20-140), contributing to 42% of diagnoses between 2011 and 2015. Herein, tissue culture selections ascertained if large cluster lineages possessed higher replicative fitness than singleton/small cluster isolates, allowing for viral escape from integrase inhibitors...
May 2, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28467485/correction-effects-of-combined-ccr5-integrase-inhibitors-based-regimen-on-mucosal-immunity-in-hiv-infected-patients-na%C3%A3-ve-to-antiretroviral-therapy-a-pilot-randomized-trial
#14
Sergio Serrano-Villar, Talia Sainz, Zhong-Min Ma, Netanya S Utay, Tae Wook-Chun, Surinder Mann, Angela D Kashuba, Basile Siewe, Anthony Albanese, Paolo Troia-Cancio, Elizabeth Sinclair, Anoma Somasunderam, Tammy Yotter, Steven G Deeks, Alan Landay, Richard B Pollard, Christopher J Miller, Santiago Moreno, David M Asmuth
[This corrects the article DOI: 10.1371/journal.ppat.1005381.].
May 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28460328/isothermal-assay-targeting-class-1-integrase-gene-for-environmental-surveillance-of-antibiotic-resistance-markers
#15
Robert D Stedtfeld, Tiffany M Stedtfeld, Hassen Waseem, Meridith Fitschen-Brown, Xueping Guo, Benli Chai, Maggie R Williams, Trevor Shook, Amanda Logan, Ally Graham, Jong-Chan Chae, Woo-Jun Sul, Jacob VanHouten, James R Cole, Gerben J Zylstra, James M Tiedje, Brad L Upham, Syed A Hashsham
Antimicrobial resistance genes (ARGs) present in the environment pose a risk to human health due to potential for transfer to human pathogens. Surveillance is an integral part of mitigating environmental dissemination. Quantification of the mobile genetic element class 1 integron-integrase gene (intI1) has been proposed as a surrogate to measuring multiple ARGs. Measurement of such indicator genes can be further simplified by adopting emerging nucleic acids methods such as loop mediated isothermal amplification (LAMP)...
April 28, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28458055/retroviral-intasomes-arising
#16
REVIEW
Alan N Engelman, Peter Cherepanov
Retroviral DNA integration takes place in the context of the intasome nucleoprotein complex. X-ray crystal structures of functional spumaviral intasomes were previously revealed to harbor a homotetramer of integrase, and it was generally believed that integrase tetramers catalyzed the integration of other retroviruses. The elucidation of new structures from four different retroviruses over the past year has however revealed this is not the case. The number of integrase molecules required to construct the conserved intasome core structure differs between viral species...
April 27, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28455670/complete-nucleotide-sequence-and-annotation-of-the-temperate-corynephage-%C3%AF-16-genome
#17
Juliya S Lobanova, Evgueni R Gak, Irina G Andreeva, Konstantin V Rybak, Alexander A Krylov, Sergey V Mashko
The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 bp (G+C = 52.2%) with protruding cohesive 3'-ends of 14 nt. Four major structural proteins were separated by SDS-PAGE and identified by peptide mass fingerprinting technique. Using bioinformatics analysis, 101 putative ORFs and 5 tRNA genes were predicted...
April 28, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28454901/a-computational-model-for-predicting-integrase-catalytic-domain-of-retrovirus
#18
Sijia Wu, Jiuqiang Han, Xinman Zhang, Dexing Zhong, Ruiling Liu
Integrase catalytic domain (ICD) is an essential part in the retrovirus for integration reaction, which enables its newly synthesized DNA to be incorporated into the DNA of infected cells. Owing to the crucial role of ICD for the retroviral replication and the absence of an equivalent of integrase in host cells, it is comprehensible that ICD is a promising drug target for therapeutic intervention. However, annotated ICDs in UniProtKB database have still been insufficient for a good understanding of their statistical characteristics so far...
April 26, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28442158/diversity-and-evolutionary-aspects-of-mucin-binding-mucbp-domain-repeats-among-lactobacillus-plantarum-group-strains-through-comparative-genetic-analysis
#19
Sundru Manjulata Devi, Prakash M Halami
Mucin binding protein (Mub) of Lactobacillus plantarum- group (LPG) is considered as a potential marker gene due to its diversity and adherence property to host intestinal layer. We recently observed strain specific diversity in mub gene of LPG strains (n=8) and grouped them into Type I, II and III based on the insertion and/or deletion of MucBP domain repeats (Devi et al., 2016). The mub gene expression pattern noted by real-time PCR revealed 67.64 increased fold change in MTCC 9483 with four MucBP repeats...
April 12, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28436334/acute-hiv-infection-advances-in-mechanisms-and-prophylactic-therapeutic-management
#20
Muhammad Imran Qadir, Amman Abid
Human infection by HIV is spreading rapidly. The spread of the virus is generally organized and transmission can be rectal, oral, or penile. Several types of cells are susceptible to viral invasion. The appearance of symptoms during infection can vary, but the presence of the virus can be confirmed by testing for the antigen p24. The ELISA test is used to detect viral p24 antigen and antiviral antibodies. This technique is approved for the purposes of clinical practice. Highly active antiretroviral therapy is usually a costly and lengthy treatment for HIV infection...
2017: Critical Reviews in Eukaryotic Gene Expression
keyword
keyword
60253
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"