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https://www.readbyqxmd.com/read/27932096/study-of-%C3%AE-2-glycoprotein-i-polymorphisms-in-patients-with-chronic-renal-failure-as-a-predisposing-factor-for-the-development-of-anti-%C3%AE-2-glycoprotein-i-auto-antibodies
#1
M Serrano, O Cabrera-Marante, J A Martínez-Flores, P Morales, D Pérez, S Mora, F García, E González, E Paz-Artal, J M Morales, A Serrano
BACKGROUND: Immunoglobulin (Ig)A anti-β2-glycoprotein I (aB2GP1) antibodies are associated with thrombotic events, cardiovascular morbidity, and death in dialysis patients. About 30% of patients with chronic renal disease are positive for IgA aB2GP1; however, the origin of these antibodies is unknown. It has been speculated that dialysis membranes, age, or etiology of renal base disease are possible precipitating factors, although these factors do not appear to be the source of antibodies...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27931514/vascular-alterations-in-a-murine-model-of-acute-graft-versus-host-disease-are-associated-with-decreased-serum-levels-of-adiponectin-and-an-increased-activity-and-vascular-expression-of-indoleamine-2-3-dioxygenase
#2
Peter M Schmid, Abdellatif Bouazzaoui, Karin Schmid, Christoph M Birner, Christian Schach, Lars S Maier, Ernst Holler, Dierk H Endemann
Graft-versus-host disease (GVHD) is the limiting complication after bone marrow transplantation (BMT), and its pathophysiology seems to be highly influenced by vascular factors. Our study aimed at elucidating possible mechanisms involved in vascular GVHD. For this purpose, we used a fully MHC-mismatched model of BALB/c mice conditioned according to two different intensity protocols with total body irradiation and transplantation of allogeneic (C57BL/6) or syngeneic bone marrow cells and splenocytes. Mesenteric resistance arteries were studied in a pressurized myograph...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27929146/reactivity-of-the-nitrogen-centered-tryptophanyl-radical-in-the-catalysis-by-the-radical-sam-enzyme-nosl
#3
Haocheng Qianzhu, Wenjuan Ji, Xinjian Ji, Leixia Chu, Chuchu Guo, Wei Lu, Wei Ding, Jiangtao Gao, Qi Zhang
The radical SAM tryptophan (Trp) lyase NosL involved in nosiheptide biosynthesis catalyzes two parallel reactions, converting l-Trp to 3-methyl-2-indolic acid (MIA) and to dehydroglycine and 3-methylindole, respectively. The two parallel reactions diverge from a nitrogen-centered tryptophanyl radical intermediate. Here we report an investigation on the intrinsic reactivity of the tryptophanyl radical using a chemical model study and DFT calculations. The kinetics of the formation and fragmentation of this nitrogen-centered radical in NosL catalysis were also studied in detail...
December 8, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27924276/mypresto-omegagene-a-gpu-accelerated-molecular-dynamics-simulator-tailored-for-enhanced-conformational-sampling-methods-with-a-non-ewald-electrostatic-scheme
#4
Kota Kasahara, Benson Ma, Kota Goto, Bhaskar Dasgupta, Junichi Higo, Ikuo Fukuda, Tadaaki Mashimo, Yutaka Akiyama, Haruki Nakamura
Molecular dynamics (MD) is a promising computational approach to investigate dynamical behavior of molecular systems at the atomic level. Here, we present a new MD simulation engine named "myPresto/omegagene" that is tailored for enhanced conformational sampling methods with a non-Ewald electrostatic potential scheme. Our enhanced conformational sampling methods, e.g., the virtual-system-coupled multi-canonical MD (V-McMD) method, replace a multi-process parallelized run with multiple independent runs to avoid inter-node communication overhead...
2016: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/27923683/role-of-a-tachykinin-related-peptide-and-its-receptor-in-modulating-the-olfactory-sensitivity-in-the-oriental-fruit-fly-bactrocera-dorsalis-hendel
#5
Shun-Hua Gui, Hong-Bo Jiang, Li Xu, Yu-Xia Pei, Xiao-Qiang Liu, Guy Smagghe, Jin-Jun Wang
Insect tachykinin-related peptide (TRP), an ortholog of tachykinin in vertebrates, has been linked with regulation of diverse physiological processes, such as olfactory perception, locomotion, aggression, lipid metabolism and myotropic activity. In this study, we investigated the function of TRP (BdTRP) and its receptor (BdTRPR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis. BdTRPR is a typical G-protein coupled-receptor (GPCR), and it could be activated by the putative BdTRP mature peptides with the effective concentrations (EC50) at the nanomolar range when expressed in Chinese hamster ovary cells...
December 3, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27920887/preliminary-study-on-cost-effective-l-tryptophan-production-from-indole-and-l-serine-by-e-coli-cells
#6
Tahereh Sadeghiyan-Rizi, Jamshid Fooladi, Sima Sadrai
BACKGROUND: L-tryptophan is used widespread in the pharmaceutical industry. The majority of L-Trp production depends on microbial processes that produce L-tryptophan from indole and L-serine. These processes are very costly due to the costs of precursors, especially L-serine. Use of inexpensive substitutions as the L-serine source of L-tryptophan production enables us to reach a cost-effective process. In this paper, effect of Triton X-100 on L-Trp production and the ability to use Iranian cane molasses as inexpensive L-serine source was investigated...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27918933/tyrosine-fluorescence-probing-of-conformational-changes-in-tryptophan-lacking-domain-of-albumins
#7
N G Zhdanova, E G Maksimov, A M Arutyunyan, V V Fadeev, E A Shirshin
We addressed the possibility of using tyrosine (Tyr) fluorescence for monitoring conformational changes of proteins which are undetectable via tryptophan (Trp) fluorescence. The model objects, human (HSA) and bovine (BSA) serum albumins, contain one and two Trp residues, respectively, while Tyr is more uniformly distributed over their structure. The results of the investigation of albumins interaction with ethanol using intrinsic Trp and Tyr steady-state and time-resolved picosecond fluorescence indicated the presence of an intermediate at 10% (v/v) of ethanol in solution, that was supported by the results of extrinsic fluorescence measurements with the Nile Red dye...
November 25, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27916815/changes-in-biochemical-parameters-of-the-calcium-phosphorus-homeostasis-in-relation-to-nutritional-intake-in-very-low-birth-weight-infants
#8
Viola Christmann, Charlotte J W Gradussen, Michelle N Körnmann, Nel Roeleveld, Johannes B van Goudoever, Arno F J van Heijst
Preterm infants are at significant risk to develop reduced bone mineralization based on inadequate supply of calcium and phosphorus (Ca-P). Biochemical parameters can be used to evaluate the nutritional intake. The direct effect of nutritional intake on changes in biochemical parameters has not been studied. Our objective was to evaluate the effect of Ca-P supplementation on biochemical markers as serum (s)/urinary (u) Ca and P; alkaline phosphatase (ALP); tubular reabsorption of P (TrP); and urinary ratios for Ca/creatinin (creat) and P/creatinin in Very-Low-Birth-Weight infants on Postnatal Days 1, 3, 5, 7, 10, and 14...
November 29, 2016: Nutrients
https://www.readbyqxmd.com/read/27915018/design-and-surface-immobilization-of-short-anti-biofilm-peptides
#9
Biswajit Mishra, Tamara Lushnikova, Radha M Golla, Xiuqing Wang, Guangshun Wang
: Short antimicrobial peptides are essential to keep us healthy and their lasting potency can inspire the design of new types of antibiotics. This study reports the design of a family of eight-residue tryptophan-rich peptides (TetraF2W) obtained by converting the four phenylalanines in temporin-SHf to tryptophans. The temporin-SHf template was identified from the antimicrobial peptide database (http://aps.unmc.edu/AP). Remarkably, the double arginine variant (TetraF2W-RR) was more effective in killing methicillin-resistant Staphylococcus aureus (MRSA) USA300, but less cytotoxic to human skin HaCat and kidney HEK293 cells, than the lysine-containing dibasic combinations (KR, RK and KK)...
November 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27913850/two-novel-cyclic-hexapeptides-from-the-genetically-engineered-actinosynnema-pretiosum
#10
Chunhua Lu, Fei Xie, Chuan Shan, Yuemao Shen
Two novel cyclic hexapeptides designated actinosynneptides A (1) and B (2), together with three tryptophan containing diketopiperazines, namely cyclo(L-Trp-L-Trp) (3), cyclo(L-Trp-N-MeL-Trp) (4), and cyclo(N-MeL-Trp-N-MeL-Trp) (5), were isolated from the culture of the genetically engineered strain HGF052::asm18 derived from Actinosynnema pretiosum ATCC31565. Their structures were elucidated on the basis of spectroscopic analyses and single-crystal X-ray diffractions. Compound 1 is the first example of 3-amino-6-hydroxy-2-piperidone-containing cyclic peptides, and 1 and 2 showed moderate cytotoxic activities against HeLa and PC3 cell lines...
December 2, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27913205/trp-channels-in-calcium-homeostasis-from-hormonal-control-to-structure-function-relationship-of-trpv5-and-trpv6
#11
REVIEW
Mark K C van Goor, Joost G J Hoenderop, Jenny van der Wijst
Maintaining plasma calcium levels within a narrow range is of vital importance for many physiological functions. Therefore, calcium transport processes in the intestine, bone and kidney are tightly regulated to fine-tune the rate of absorption, storage and excretion. The TRPV5 and TRPV6 calcium channels are viewed as the gatekeepers of epithelial calcium transport. Several calciotropic hormones control the channels at the level of transcription, membrane expression, and function. Recent technological advances have provided the first near-atomic resolution structural models of several TRPV channels, allowing insight into their architecture...
November 30, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27911256/a-residue-located-at-the-junction-of-the-head-and-stalk-regions-of-measles-virus-fusion-protein-regulates-membrane-fusion-by-controlling-conformational-stability
#12
Yuto Satoh, Saeka Yonemori, Mitsuhiro Hirose, Hiroko Shogaki, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Tsuyoshi Shirai, Ken-Ichi Takahashi, Masae Itoh
The fusion (F) protein of measles virus (MV) performs refolding from the thermodynamically metastable prefusion form to the highly stable postfusion form via an activated unstable intermediate stage, to induce membrane fusion. Some amino acids involved in the fusion regulation cluster in the heptad repeat B (HR-B) domain of the stalk region, among which substitution of residue 465 by various amino acids revealed that fusion activity correlates well with its side chain length from the Cα (p<0.01) and van der Waals volume (p<0...
December 1, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27910732/binding-of-anti-cardiovascular-drug-to-serum-albumin-an-insight-in-the-light-of-spectroscopic-and-computational-approaches
#13
Tajalli Ilm Chandel, Gulam Rabbani, MohsinVahid Khan, Masihuz Zaman, Parvez Alam, Yasser Shahein, Rizwan Hasan Khan
Isoprenaline hydrochloride is a potential cardiovascular drug helps in the smooth functioning of the heart muscles. So, we have performed the binding study of ISO with BSA. This study was investigated by UV absorption, fluorescence, synchronous fluorescence, circular dichroism etc. The analysis of intrinsic fluorescence data showed the low binding affinity of ISO. The binding constant Kb was 2.8×103 M-1 and binding stoichiometry (n) was approximately one and the Gibb's free energy change at 310 K was determined to be -8...
December 2, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27909054/identification-of-a-substrate-selective-exosite-within-the-metalloproteinase-anthrax-lethal-factor
#14
Allison B Goldberg, Eunice Cho, Chad J Miller, Hua Jane Lou, Benjamin E Turk
The metalloproteinase anthrax lethal factor (LF) is secreted by Bacillus anthracis to promote disease virulence through disruption of host signaling pathways. LF is a highly specific protease, exclusively cleaving mitogen-activated protein kinase kinases (MKKs) and rodent NACHT leucine-rich repeat and pyrin domain containing protein 1B (NLRP1B). How LF achieves such restricted substrate specificity is not understood. Previous studies have suggested the existence of an exosite interaction between LF and MKKs that promotes cleavage efficiency and specificity...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27906239/fiber-optic-electron-spin-resonance-thermometry-of-single-laser-activated-neurons
#15
A A Lanin, I V Fedotov, Yu G Ermakova, D A Sidorov-Biryukov, A B Fedotov, P Hemmer, V V Belousov, A M Zheltikov
Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca<sup>2+</sup> current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels...
December 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27903740/protective-t-cell-responses-featured-by-concordant-recognition-of-middle-east-respiratory-syndrome-coronavirus-derived-cd8-t-cell-epitopes-and-host-mhc
#16
William J Liu, Jiaming Lan, Kefang Liu, Yao Deng, Yanfeng Yao, Shaolian Wu, Hong Chen, Lingling Bao, Haifeng Zhang, Min Zhao, Qihui Wang, Lingxia Han, Yan Chai, Jianxun Qi, Jincun Zhao, Songdong Meng, Chuan Qin, George F Gao, Wenjie Tan
The coordinated recognition of virus-derived T cell epitopes and MHC molecules by T cells plays a pivotal role in cellular immunity-mediated virus clearance. It has been demonstrated that the conformation of MHC class I (MHC I) molecules can be adjusted by the presented peptide, which impacts T cell activation. However, it is still largely unknown whether the conformational shift of MHC I influences the protective effect of virus-specific T cells. In this study, utilizing the Middle East respiratory syndrome coronavirus-infected mouse model, we observed that through the unusual secondary anchor Ile5, a CD8(+) T cell epitope drove the conformational fit of Trp(73) on the α1 helix of murine MHC I H-2K(d) In vitro renaturation and circular dichroism assays indicated that this shift of the structure did not influence the peptide/MHC I binding affinity...
November 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27898965/performance-of-pigs-kept-under-different-sanitary-conditions-affected-by-protein-intake-and-amino-acid-supplementation
#17
Y van der Meer, A Lammers, A J M Jansman, M M J A Rijnen, W H Hendriks, W J J Gerrits
There is growing evidence that requirements for particular AA increase when pigs are kept under low sanitary conditions. The extent to which reduction in growth performance is related to these increased requirements is unclear. To evaluate this relationship, an experiment (2 × 2 × 2 factorial arrangement) was performed with 612 male pigs (9 per pen) kept under low sanitary conditions (LSC) or high sanitary conditions (HSC) and offered ad libitum access to either a normal CP concentration diet (NP; 17, 15, and 15% CP for the starter, grower, and finisher phase, respectively) or a low CP concentration diet (LP; 20% CP reduced relative to NP for each phase), each of which containing a basal AA profile (AA-B) or a supplemented AA profile (AA-S)...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898953/impact-of-improving-dietary-amino-acid-balance-for-lactating-sows-on-efficiency-of-dietary-amino-acid-utilization-and-transcript-abundance-of-genes-encoding-lysine-transporters-in-mammary-tissue
#18
L Huber, C F M de Lange, C W Ernst, U Krogh, N L Trottier
Lactating multiparous Yorkshire sows ( = 64) were used in 2 experiments to test the hypothesis that reducing dietary CP intake and improving AA balance through crystalline AA (CAA) supplementation improves apparent dietary AA utilization efficiency for milk production and increases transcript abundance of genes encoding Lys transporter proteins in mammary tissue. In Exp. 1, 40 sows were assigned to 1 of 4 diets: 1) high CP (HCP; 16.0% CP, as-fed basis; analyzed concentration), 2) medium-high CP (MHCP; 15.7% CP), 3) medium-low CP (MLCP; 14...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898917/chemical-composition-and-standardized-ileal-amino-acid-digestibility-of-eight-genotypes-of-rye-fed-to-growing-pigs
#19
E J P Strang, M Eklund, P Rosenfelder, N Sauer, J K Htoo, R Mosenthin
A study with growing pigs was conducted to determine the chemical composition, physical characteristics, and standardized ileal digestibility (SID) of CP and AA of 8 rye genotypes that have recently been added to the German Descriptive Variety List. Eight barrows with an initial BW of 24 ± 2 kg were fitted with T-cannulas at the distal ileum and allotted to an 8 × 8 Latin square design with 8 periods of 6 d each and 8 pigs receiving 8 different diets. The N-free method was used to determine the basal ileal endogenous CP and AA losses...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898914/postprandial-portal-fluxes-of-essential-amino-acids-volatile-fatty-acids-and-urea-nitrogen-in-growing-pigs-fed-a-high-fiber-diet-supplemented-with-a-multi-enzyme-cocktail
#20
A K Agyekum, E Kiarie, M C Walsh, C M Nyachoti
The present study investigated the effects of adding a multi-enzyme cocktail (MC) to a high-fiber diet on net portal-drained viscera (PDV) fluxes of essential AA (EAA), volatile fatty acids (VFA), and blood urea-N (BUN) in growing pigs. Five female pigs (22.8 ± 1.6 kg BW), with catheters in the portal vein, ileal vein, and carotid artery, were fed 3 isocaloric-nitrogenous diets at 4% of their BW once daily at 0900 h for 7 d in a replicated 3 × 3 Latin square design. The diets contained corn and soybean meal with 0% (control) or 30% distillers' dried grains with solubles (DDGS; HF) produced from a 1:1 mixture of wheat and corn...
September 2016: Journal of Animal Science
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