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https://www.readbyqxmd.com/read/29791811/the-putative-transient-receptor-potential-trp-channel-protein-encoded-by-the-orf19-4805-is-involved-in-cation-sensitivity-antifungal-tolerance-and-filamentation-in-candida-albicans
#1
Linghuo Jiang, Yi Yang
The ancient family of cation channels, transient receptor potential (TRP) channels, is highly conserved in eukaryotes and plays various physiological functions from sensation of ion homeostasis to reception of pain and vision. Calcium-permeable TRP channels have been identified from the plant Arabidopsis thaliana (AtCsc1) and the budding yeast Saccharomyces cerevisiae (ScCsc1), respectively. In this study, we have characterized the functions of the Csc1 homolog, the orf19.4805, in Candida albicans. The orf19...
May 23, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29791145/slow-starter-enzymes-role-of-active-site-architecture-in-the-catalytic-control-in-the-biosynthesis-of-taxadiene-by-taxadiene-synthase
#2
Tamar Ansbacher, Yehoshua Freud, Dan Thomas Major
Taxadiene synthase (TXS) catalyzes the formation of the natural product Taxa-4(5),11(12)-diene (henceforth Taxadiene). Taxadiene is the precursor in the formation of Taxol, which is an important natural anti-cancer agent. In the current study, we present a detailed mechanistic view of the biosynthesis of Taxadiene by TXS, using a hybrid quantum mechanics-molecular mechanics potential in conjunction with free energy simulation methods. The obtained free energy landscape displays initial endergonic steps followed by a step-wise downhill profile, which is an emerging free energy fingerprint for type I terpene synthases...
May 23, 2018: Biochemistry
https://www.readbyqxmd.com/read/29785460/rapid-measurement-of-long-range-distances-in-proteins-by-multidimensional-13-c-19-f-redor-nmr-under-fast-magic-angle-spinning
#3
Alexander A Shcherbakov, Mei Hong
The ability to simultaneously measure many long-range distances is critical to efficient and accurate determination of protein structures by solid-state NMR (SSNMR). So far, the most common distance constraints for proteins are 13 C-15 N distances, which are usually measured using the rotational-echo double-resonance (REDOR) technique. However, these measurements are restricted to distances of up to ~ 5 Å due to the low gyromagnetic ratios of 15 N and 13 C. Here we present a robust 2D 13 C-19 F REDOR experiment to measure multiple distances to ~ 10 Å...
May 21, 2018: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/29784393/interactions-of-pyrene-and-or-1-hydroxypyrene-with-bovine-serum-albumin-based-on-eem-parafac-combined-with-molecular-docking
#4
Jing Zhang, Linfeng Chen, Dan Liu, Yaxian Zhu, Yong Zhang
The interactions of pyrene (Pyr) and/or 1-hydroxypyrene (1-OHPyr) with bovine serum albumin (BSA) in binary and ternary systems were investigated using the excitation-emission matrix (EEM)-parallel factor analysis (PARAFAC) method combined with fluorescence quenching analysis and the molecular docking method. The results showed that the PARAFAC approach could be used to decompose the EEM spectra of Pyr, 1-OHPyr, and BSA in the binary and ternary systems. The binding constants of Pyr and 1-OHPyr with BSA increased from 1...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784339/quantification-of-peptides-in-human-synovial-fluid-using-liquid-chromatography-tandem-mass-spectrometry
#5
Araceli Garcia-Ac, Sung Vo Duy, Sébastien Sauvé, Florina Moldovan, V Gaëlle Roullin, Xavier Banquy
A method to explore the stability of two anti-inflammatory peptides in human synovial fluid (HSF) has been developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The two peptides are BQ123 Cyclo(-D-Trp-D-Asp-L-Pro-D-Val-L-Leu, Mw = 610.7) and R-954 (AcOrn[Oic2 , (αMe)Phe5 , DβNal7 , Ile8 ]desArg9 -bradykinin, Mw = 1194.4). Human synovial fluid samples were analyzed after a protein precipitation step with acetonitrile and dilution with mobile phase. DMSO was used as anti-adsorptive agent...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783840/discovery-of-melanocortin-ligands-via-a-double-simultaneous-substitution-strategy-based-on-the-ac-his-dphe-arg-trp-nh-2-template
#6
Aleksandar Todorovic, Cody J Lensing, Jerry Ryan Holder, Joseph W Scott, Nicholas B Sorensen, Carrie Haskell-Luevano
The melanocortin system regulates an array of diverse physiological functions including pigmentation, feeding behavior, energy homeostasis, cardiovascular regulation, sexual function, and steroidogenesis. Endogenous melanocortin agonist ligands all possess the minimal messaging tetrapeptide sequence His-Phe-Arg-Trp. Based on this endogenous sequence, the Ac-His1-DPhe2-Arg3-Trp4-NH2 tetrapeptide has previously been shown to be a useful scaffold when utilizing traditional positional scanning approaches to modify activity at the various melanocortin receptors (MC1-5R)...
May 21, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29782837/phenylalanine-and-tryptophan-stimulate-gastrin-and-somatostatin-secretion-and-h-k-atpase-activity-in-pigs-through-calcium-sensing-receptor
#7
Yihan Xian, Xiuying Zhao, Chao Wang, Cuicui Kang, Liren Ding, Weiyun Zhu, Suqin Hang
In rodents and humans, aromatic amino acids increase gut hormone secretion and H+ -K+ -ATPase activity by modulating calcium-sensing receptor (CaSR). However, the role of CaSR and its related signaling molecules in amino acid-induced gut hormone secretion in swine has not been investigated. Here, we examined whether a CaSR-dependent pathway modulated gastrin and somatostatin (SS) secretion and H+ -K+ -ATPase activity in pigs. Perfusion of pig stomach tissues in the presence of extracellular 80 mM l-phenylalanine (Phe) or 20 mM l-tryptophan (Trp) and a CaSR agonist cinacalcet triggered gastrin and SS secretion and H+ -K+ -ATPase activity (P < 0...
May 18, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29779467/calcium-sodium-and-trp-channel-expression-in-human-fetal-midbrain-derived-neural-progenitor-cells
#8
Nancy Stanslowsky, Salini Tharmarasa, Selma Staege, Norman Kalmbach, Martin Klietz, Sigrid Schwarz, Andreas Leffler, Florian Wegner
Voltage-gated sodium and calcium channels as well as transient receptor potential (TRP) channels are expressed during the differentiation of human neural progenitor cells (hNPCs) and are likely to be involved in regulating neurogenesis. However, the molecular composition of these ion channels in proliferating and differentiating hNPCs is largely unknown. Here, we investigated fetal mesencephalic hNPCs in respect to their sodium, calcium, and TRP channel subunit expression and function. Quantitative real-time PCR indicated a significant upregulation of voltage-gated sodium and calcium channel subunits in hNPCs after differentiation for 3 weeks in vitro...
May 19, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29779025/differential-determinants-of-tubular-phosphate-reabsorption-insights-on-renal-excretion-of-phosphates-in-kidney-disease
#9
Nahid Tabibzadeh, Romuald Mentaverri, Maïté Daroux, Rafik Mesbah, Alexia Delpierre, Jean-Gabriel Paul, Valérie Deken, Ziad A Massy, Pierre Bataille
We assessed the tubular reabsorption of phosphate (TRP) and maximal renal threshold for phosphate reabsorption to glomerular filtration rate (TmPi/GFR) and their determinants in 64 stages 2-4 chronic kidney disease (CKD) patients in order to define the early changes in phosphate metabolism in CKD. In multivariable analysis, TmPi/GFR correlates were estimated GFR (eGFR), intact parathyroid hormone (iPTH), and hemoglobin (R2 = 0.417), while TRP correlates were eGFR, iPTH, 24-h phosphaturia, and calcitriol (R2 = 0...
May 18, 2018: American Journal of Nephrology
https://www.readbyqxmd.com/read/29779023/parathyroid-hormone-fibroblast-growth-factor-23-and-parameters-of-phosphate-reabsorption
#10
Kenneth R Phelps, Darius L Mason
BACKGROUND: The serum phosphorus concentration ([P]s) is the sum of EP/Ccr and TRP/Ccr, where Ccr is creatinine clearance and EP and TRP are rates of excretion and reabsorption of phosphate. In chronic kidney disease (CKD), parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) mediate reduction of TRP/Ccr, and [PTH] and [FGF23] are linear functions of EP/Ccr. If controls and patients with CKD are considered together, TRP/Ccr is a hyperbolic function of EP/Ccr. Given these observations, we hypothesized that hyperbolas would describe relationships of phosphate reabsorption to [PTH] and [FGF23]...
May 18, 2018: American Journal of Nephrology
https://www.readbyqxmd.com/read/29777637/thymol-inhibits-oral-squamous-cell-carcinoma-growth-via-mitochondria-mediated-apoptosis
#11
Jorge J De La Chapa, Prajjal Kanti Singha, Debbie R Lee, Cara B Gonzales
BACKGROUND: Thymol is a Transient Receptor Potential Ankyrin Subtype 1 channel, (TRPA1) agonist found in thyme and oregano. Thymol has antioxidant, anti-inflammatory, and antimicrobial properties; thus thymol is added to many commercially available products including Listerine mouthwash. Thymol is also cytotoxic to HL-60 (acute promyelotic leukemia) cells in vitro. Therefore, we evaluated the effects of thymol against oral squamous cell carcinoma (OSCC) and its anticancer mechanism-of-action...
May 19, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29775068/embedding-a-metal-binding-motif-for-copper-transporter-into-a-lipid-bilayer-by-cu-i-binding
#12
Mariko Okada, Shinji Kajimoto, Takakazu Nakabayashi
Peptide-lipid interactions are widely involved with biologically significant phenomena, including the pathogenic mechanisms of protein misfolding diseases, and transmembrane protein folding. In this paper, the interaction of the cysteine/tryptophan (Cys/Trp) motif, which is a metal binding motif of copper transporter (Ctr) proteins, with a lipid bilayer was studied using fluorescence and circular dichroism (CD) spectroscopy. The binding of Cu(I) to the Cys/Trp motif induced a large red-edge excitation shift (REES) in the Trp fluorescence, indicating that the Trp residue is located inside the lipid bilayer following complexation of Cu(I) with the Cys/Trp motif...
May 18, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29773815/the-trimeric-solution-structure-and-fucose-binding-mechanism-of-the-core-fucosylation-specific-lectin-phosl
#13
Kazuhiko Yamasaki, Tomoko Yamasaki, Hiroaki Tateno
The core α1-6 fucosylation-specific lectin from a mushroom Pholiota squarrosa (PhoSL) is a potential tool for precise diagnosis of cancers. This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel filtration and chemical cross-linking assays, and determined a structure of the PhoSL trimer by NMR. The structure possesses a β-prism motif with a three-fold rotational symmetry, where three antiparallel β-sheets are tightly connected by swapping of β-strands...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772843/the-role-of-trp-channels-in-the-metastatic-cascade
#14
REVIEW
Benedikt Fels, Etmar Bulk, Zoltán Pethő, Albrecht Schwab
A dysregulated cellular Ca2+ homeostasis is involved in multiple pathologies including cancer. Changes in Ca2+ signaling caused by altered fluxes through ion channels and transporters (the transportome) are involved in all steps of the metastatic cascade. Cancer cells thereby "re-program" and "misuse" the cellular transportome to regulate proliferation, apoptosis, metabolism, growth factor signaling, migration and invasion. Cancer cells use their transportome to cope with diverse environmental challenges during the metastatic cascade, like hypoxic, acidic and mechanical cues...
May 17, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29772716/kinetic-basis-of-the-bifunctionality-of-ssoii-dna-methyltransferase
#15
Nadezhda A Timofeyeva, Alexandra Yu Ryazanova, Maxim V Norkin, Tatiana S Oretskaya, Olga S Fedorova, Elena A Kubareva
Type II restriction⁻modification (RM) systems are the most widespread bacterial antiviral defence mechanisms. DNA methyltransferase SsoII (M.SsoII) from a Type II RM system SsoII regulates transcription in its own RM system in addition to the methylation function. DNA with a so-called regulatory site inhibits the M.SsoII methylation activity. Using circular permutation assay, we show that M.SsoII monomer induces DNA bending of 31° at the methylation site and 46° at the regulatory site. In the M.SsoII dimer bound to the regulatory site, both protein subunits make equal contributions to the DNA bending, and both angles are in the same plane...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29772373/a-novel-c-type-lectin-from-the-sea-cucumber-apostichopus-japonicus-ajctl-2-with-preferential-binding-of-d-galactose
#16
Hui Wang, Zhuang Xue, Zhaoqun Liu, Weilin Wang, Feifei Wang, Ying Wang, Lingling Wang, Linsheng Song
C-type lectins (CTLs) are Ca2+ dependent carbohydrate-binding proteins that share structural homology in their carbohydrate-recognition domains (CRDs). In the present study, a novel CTL was identified from sea cucumber Apostichopus japonicus (named as AjCTL-2). The deduced amino acid sequence of AjCTL-2 was homologous to CTLs from other animals with the identities ranging from 33% to 40%. It contained a canonical signal peptide at the N-terminus, a low density lipoprotein receptor class A (LDLa), a C1r/C1s/Uegf/bone morphogenetic protein 1 (CUB), and a CRD with two motif Glu-Pro-Asn (EPN) and Trp-Asn-Asp (WND) in Ca2+ binding site 2...
May 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29772173/quantitative-surface-enhanced-raman-spectroscopy-through-the-interface-assisted-self-assembly-of-three-dimensional-silver-nanorod-substrates
#17
Siying Liu, Xiang-Dong Tian, Yun Zhang, Jian-Feng Li
The realization of surface-enhanced Raman spectroscopy (SERS) to be a reliable quantitative analytical technique requires sensitive and reproducible enhancing substrates. Here, uniform three-dimensional (3D) Ag nanorod (AgNR) substrates with well-defined interlayer spacings are prepared through the air-liquid interface-assisted self-assembly of AgNR in a layer-by-layer manner. The correlation of the SERS performance with the 3D AgNR structures is performed by SERS mapping the substrates. SERS mapping reveals the excellent enhancement uniformity of the 3D substrates with the relative standard deviation (RSD) less than 10%...
May 17, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29767241/transcriptome-sequencing-analysis-reveals-the-effect-of-combinative-treatment-with-low%C3%A2-intensity-pulsed-ultrasound-and-magnesium-ions-on-hfob1-19-human-osteoblast-cells
#18
Haiyue Zu, Xueting Yi, Dewei Zhao
Biodegradable magnesium (Mg) materials are considered ideal as osteosynthesis implants. However, clinical application has proven complex. This is primarily associated with the issue of reducing the extent of implant degradation to a range acceptable for the human body, while simultaneously enhancing osteogenesis or osteoinduction. In the present study, a combination of Mg ions and low‑intensity pulsed ultrasound (LIPUS) treatment was applied in hFOB 1.19 human osteoblast cells as a potential strategy to resolve this issue...
May 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29763760/multispectroscopic-and-bioimaging-approach-for-the-interaction-of-rhodamine-6g-capped-gold-nanoparticles-with-bovine-serum-albumin
#19
N Manjubaashini, Mookkandi Palsamy Kesavan, Jegathalaprathaban Rajesh, T Daniel Thangadurai
Binding interaction of Bovine Serum Albumin (BSA) with newly prepared rhodamine 6G-capped gold nanoparticles (Rh6G-Au NPs) under physiological conditions (pH 7.2) was investigated by a wide range of photophysical techniques. Rh6G-Au NPs caused the static quenching of the intrinsic fluorescence of BSA that resulted from the formation of ground-state complex between BSA and Rh6G-Au NPs. The binding constant from fluorescence quenching method (Ka  = 1.04 × 104  L mol-1 ; LoD = 14.0 μM) is in accordance with apparent association constant (Kapp  = 1...
May 7, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29758318/proteome-and-behavioral-alternations-in-phosphorylation-deficient-mutant-collapsin-response-mediator-protein2-knock-in-mice
#20
Haruko Nakamura, Aoi Takahashi-Jitsuki, Hiroko Makihara, Tetsuya Asano, Yayoi Kimura, Jun Nakabayashi, Naoya Yamashita, Yuko Kawamoto, Fumio Nakamura, Toshio Ohshima, Hisashi Hirano, Fumiaki Tanaka, Yoshio Goshima
CRMP2, alternatively designated as DPYSL2, was the first CRMP family member to be identified as an intracellular molecule mediating the signaling of the axon guidance molecule Semaphorin 3A (Sema3A). In Sema3A signaling, cyclin-dependent kinase 5 (Cdk5) primarily phosphorylates CRMP2 at Ser522. Glycogen synthase kinase-3β (GSK-3β) subsequently phosphorylates the residues of Thr509 and Thr514 of CRMP2. Previous studies showed that CRMP2 is involved in pathogenesis of neurological disorders such as Alzheimer's disease...
May 11, 2018: Neurochemistry International
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