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Kidney, growth and development, technology,

Matthew B Coppock, Candice R Warner, Brandi Dorsey, Joshua A Orlicki, Deborah A Sarkes, Bert T Lai, Suresh M Pitram, Rosemary D Rohde, Jacquie Malette, Jeré A Wilson, Paul Kearney, Kenneth C Fang, Scott M Law, Sherri L Candelario, Blake Farrow, Amethist S Finch, Heather D Agnew, James R Heath, Dimitra N Stratis-Cullum
We report on peptide-based ligands matured through the Protein Catalyzed Capture (PCC) agent method to tailor molecular binders for in vitro sensing/diagnostics and in vivo pharmacokinetics parameters. A Vascular Endothelial Growth Factor (VEGF) binding peptide and a peptide against the Protective Antigen (PA) protein of Bacillus anthracis discovered through phage and bacterial display panning technologies, respectively, were modified with click handles and subjected to iterative in situ click chemistry screens using synthetic peptide libraries...
August 19, 2016: Biopolymers
Frans Schutgens, Marianne C Verhaar, Maarten B Rookmaaker
Organoids are self-organizing multicellular structures that contain multiple cell types, represent organ structure and function, and can be used to model organ development, maintenance and repair ex vivo. Organoid cultures, derived from embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) or adult stem cells, and cultured in extracellular matrix (ECM), have been developed for multiple organs. For the kidney, pluripotent stem cell (PSCs) derived organoid technology has rapidly developed in the last three years...
June 30, 2016: European Journal of Pharmacology
Nafiseh Poornejad, Lara B Schaumann, Evan M Buckmiller, Nima Momtahan, Jason R Gassman, Ho Hin Ma, Beverly L Roeder, Paul R Reynolds, Alonzo D Cook
The combination of patient-specific cells with scaffolds obtained from natural sources may result in improved regeneration of human tissues. Decellularization of the native tissue is the first step in this technology. Effective decellularization uses agents that lyse cells and remove all cellular materials, leaving intact collagenous extracellular matrices (ECMs). Removing cellular remnants prevents an immune response while preserving the underlying structure. In this study, the impact of five decellularization agents (0...
June 16, 2016: Journal of Biomaterials Applications
Pirada Suphaphiphat, Lynne Whittaker, Ivna De Souza, Rodney S Daniels, Philip R Dormitzer, John W McCauley, Ethan C Settembre
The global system for manufacturing seasonal influenza vaccines has been developed to respond to the natural evolution of influenza viruses, but the problem of antigenic mismatch continues to be a challenge in certain years. In some years, mismatches arise naturally due to the antigenic drift of circulating viruses after vaccine strain selection has already been made. In other years, antigenic differences between the vaccine virus and circulating viruses are introduced as part of the current system, which relies on the use of egg-adapted isolates as a starting material for candidate vaccine viruses (CVVs)...
July 12, 2016: Vaccine
Deyou Ma, Aijun Ma, Zhihui Huang, Guangning Wang, Ting Wang, Dandan Xia, Benhe Ma
BACKGROUND: Turbot Scophthalmus maximus is an economically important species extensively aquacultured in China. The genetic selection program is necessary and urgent for the sustainable development of this industry, requiring more and more genome background knowledge. Transcriptome sequencing is an excellent alternative way to identify transcripts involved in specific biological processes and exploit a considerable quantity of molecular makers when no genome sequences are available. In this study, a comprehensive transcript dataset for major tissues of S...
2016: PloS One
Cynthia A Batchelder, Michele L Martinez, Alice F Tarantal
Despite the enthusiasm for bioengineering of functional renal tissues for transplantation, many obstacles remain before the potential of this technology can be realized in a clinical setting. Viable tissue engineering strategies for the kidney require identification of the necessary cell populations, efficient scaffolds, and the 3D culture conditions to develop and support the unique architecture and physiological function of this vital organ. Our studies have previously demonstrated that decellularized sections of rhesus monkey kidneys of all age groups provide a natural extracellular matrix (ECM) with sufficient structural properties with spatial and organizational influences on human embryonic stem cell (hESC) migration and differentiation...
2015: PloS One
L Chen, J Yue, X Han, J Li, Y Hu
Intrauterine growth restriction (IUGR) is associated with a reduction in the numbers of nephrons in neonates, which increases the risk of hypertension. Our previous study showed that ouabain protects the development of the embryonic kidney during IUGR. To explore this molecular mechanism, IUGR rats were induced by protein and calorie restriction throughout pregnancy, and ouabain was delivered using a mini osmotic pump. RNA sequencing technology was used to identify the differentially expressed genes (DEGs) of the embryonic kidneys...
February 2016: Journal of Developmental Origins of Health and Disease
Yanhua Su, Jiansheng Xie, Shanshan Yu, Hongyu Luo, Weiqing Wu, Zhiyong Xu
OBJECTIVE: To confirm the genetic diagnosis for providing services for genetic counseling and prenatal diagnosis, we analyzed the clinical and genetic data of a pedigree which is clinically diagnosed as Joubert syndrome. METHOD: A Joubert syndrome pedigree was enrolled as subject of this study from our hospital's outpatients in 2013. Following the medical history collection of the proband and the suffering fetus, target sequence capture and the next-generation sequencing technology were used for the proband and the suffering fetus to find the causative genes and sanger sequencing for the members of the pedigree to check and verify if the inherited mutations are in accordance with the Mendelian inheritance...
June 2015: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Jianfeng Guo, Sharon L McKenna, Michael E O'Dwyer, Mary R Cahill, Caitriona M O'Driscoll
INTRODUCTION: Multiple myeloma (MM) is a hematological malignancy characterized by infiltration of malignant plasma cells in the bone marrow (BM) and end-organ damage to the bone, BM, kidney and immune system. Although current treatments have improved the treatment of MM, it still remains an incurable disease. RNA interference (RNAi) effectors such as microRNAs and small interference RNAs have shown potential to selectively downregulate genes implicated in the pathology of a range of diseases...
2016: Expert Opinion on Therapeutic Targets
Frederique Rodieux, Melanie Wilbaux, Johannes N van den Anker, Marc Pfister
Neonates, infants, and children differ from adults in many aspects, not just in age, weight, and body composition. Growth, maturation and environmental factors affect drug kinetics, response and dosing in pediatric patients. Almost 80% of drugs have not been studied in children, and dosing of these drugs is derived from adult doses by adjusting for body weight/size. As developmental and maturational changes are complex processes, such simplified methods may result in subtherapeutic effects or adverse events...
December 2015: Clinical Pharmacokinetics
Marpadga A Reddy, Rama Natarajan
The growing epidemic of obesity and diabetes, the aging population as well as prevalence of drug abuse has led to significant increases in the rates of the closely associated acute and chronic kidney diseases, including diabetic nephropathy. Furthermore, evidence shows that parental behavior and diet can affect the phenotype of subsequent generations via epigenetic transmission mechanisms. These data suggest a strong influence of the environment on disease susceptibility and that, apart from genetic susceptibility, epigenetic mechanisms need to be evaluated to gain critical new information about kidney diseases...
August 2015: Kidney International
Takashi Hirukawa, Takatoshi Kakuta, Michio Nakamura, Masafumi Fukagawa
Given the advances in medical technologies related to kidney transplantation, the post-transplant graft survival rate and quality of life have improved dramatically. Nevertheless, post-transplant mortality rate still remains high as compared to the general population due to the development of cardiovascular events. It has recently been widely recognized that chronic kidney disease-mineral and bone disorders (CKD-MBD) significantly contribute to such poor prognosis at least in part. In the majority of kidney recipients, abnormal serum parameters for mineral and bone disorder (MBD), such as phosphorus, calcium, 1,25-dihydroxyvitamin D, parathyroid hormone and fibroblast growth factor 23, gradually return toward acceptable levels following the re-establishment of kidney function after transplantation; however, some irreversible abnormalities, developed as the result of long-term dialysis, persist, require treatment, or even progress after kidney transplantation...
August 2015: Clinical and Experimental Nephrology
M Caralt, J S Uzarski, S Iacob, K P Obergfell, N Berg, B M Bijonowski, K M Kiefer, H H Ward, A Wandinger-Ness, W M Miller, Z J Zhang, M M Abecassis, J A Wertheim
The ability to generate patient-specific cells through induced pluripotent stem cell (iPSC) technology has encouraged development of three-dimensional extracellular matrix (ECM) scaffolds as bioactive substrates for cell differentiation with the long-range goal of bioengineering organs for transplantation. Perfusion decellularization uses the vasculature to remove resident cells, leaving an intact ECM template wherein new cells grow; however, a rigorous evaluative framework assessing ECM structural and biochemical quality is lacking...
January 2015: American Journal of Transplantation
Hiroshi Yukawa, Shingo Nakagawa, Yasuma Yoshizumi, Masaki Watanabe, Hiroaki Saito, Yoshitaka Miyamoto, Hirofumi Noguchi, Koichi Oishi, Kenji Ono, Makoto Sawada, Ichiro Kato, Daisuke Onoshima, Momoko Obayashi, Yumi Hayashi, Noritada Kaji, Tetsuya Ishikawa, Shuji Hayashi, Yoshinobu Baba
Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order to detect and trace the transplanted stem cells in the body, non-invasive and widely clinically available cell imaging technologies are required. In this paper, we focused on magnetic resonance (MR) imaging technology, and investigated whether the trimethylamino dextran-coated magnetic iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used for labeling adipose tissue-derived stem cells (ASCs) as a contrast agent...
2014: PloS One
C Fung, A Brown, J Cox, C Callaway, R McKnight, R Lane
Rodents, particularly rats, are used in the majority of intrauterine growth restriction (IUGR) research. An important tool that is lacking in this field is the ability to impose IUGR on transgenic mice. We therefore developed a novel mouse model of chronic IUGR using U-46619, a thromboxane A2 (TXA2) analog, infusion. TXA2 overproduction is prevalent in human pregnancies complicated by cigarette smoking, diabetes mellitus and preeclampsia. In this model, U-46619 micro-osmotic pump infusion in the last week of C57BL/6J mouse gestation caused maternal hypertension...
October 2011: Journal of Developmental Origins of Health and Disease
Mohammad Ayaz Hossain, Tina Chowdhury, Atul Bagul
Bone marrow stromal cells exist as mesenchymal stromal cells (MSCs) and have the capacity to differentiate into multiple tissue types when subjected to appropriate culture conditions. This property of MSCs creates therapeutic opportunities in regenerative medicine for the treatment of damage to neural, cardiac and musculoskeletal tissues or acute kidney injury. The prerequisite for successful cell therapy is delivery of cells to the target tissue. Assessment of therapeutic outcomes utilize traditional methods to examine cell function of MSC populations involving routine biochemical or histological analysis for cell proliferation, protein synthesis and gene expression...
November 2015: Journal of Tissue Engineering and Regenerative Medicine
Amir H Davarpanah, Gary M Israel
MR imaging has proven to be a versatile modality in evaluation of the kidneys, collecting system, and adrenal glands. By performing a comprehensive MR examination, it is not only possible to accurately characterize cystic and solid lesions of the kidneys, as well as urothelial masses, but also to provide important preoperative information to the surgeon. In addition, MR imaging can characterize many adrenal lesions and can frequently obviate biopsy. The continued development and growth of MR technology combined with the current trend toward minimally invasive surgery will expand the role of MR imaging in the future...
July 2014: Radiologic Clinics of North America
Ravi Katari, Andrea Peloso, Joao Paulo Zambon, Shay Soker, Robert J Stratta, Anthony Atala, Giuseppe Orlando
BACKGROUND: In 2012, about 16,487 people received kidney transplants in the USA whereas 95,022 candidates were on the waiting list at the end of the year. Moreover, more than 2,600 kidneys procured annually for transplantation are discarded for a variety of reasons. We hypothesize that this pool of discarded kidneys could in part meet the growing, urgent need for transplantable kidneys using current methods for organ bioengineering and regeneration and surgical transplantation. The recellularization of extracellular matrix (ECM) scaffolds has the potential to meet the uniquely ambitious engineering challenges posed by complex solid organs such as the kidney...
2014: Nephron. Experimental Nephrology
So Young Park, Hyun Uk Lee, Eun Sik Park, Soon Chang Lee, Jae-Won Lee, Soon Woo Jeong, Chi Hyun Kim, Young-Chul Lee, Yun Suk Huh, Jouhahn Lee
We have developed a simple approach for the large-scale synthesis of water-soluble green carbon nanodots (G-dots) from many kinds of large food waste-derived sources. About 120 g of G-dots per 100 kg of food waste can be synthesized using our simple and environmentally friendly synthesis approach. The G-dots exhibit a high degree of solubility in water because of the abundant oxygen-containing functional groups around their surface. The narrow band of photoluminescence emission (400-470 nm) confirms that the size of the G-dots (∼4 nm) is small because of a similar quantum effects and emission traps on the surfaces...
March 12, 2014: ACS Applied Materials & Interfaces
Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Pörtner, Peter Czermak
Modern bioprocesses demand for a careful definition of the critical process parameters (CPPs) already during the early stages of process development in order to ensure high-quality products and satisfactory yields. In this context, online monitoring tools can be applied to recognize unfavorable changes of CPPs during the production processes and to allow for early interventions in order to prevent losses of production batches due to quality issues. Process analytical technologies such as the dielectric spectroscopy or focused beam reflectance measurement (FBRM) are possible online monitoring tools, which can be applied to monitor cell growth as well as morphological changes...
2014: Methods in Molecular Biology
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