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https://www.readbyqxmd.com/read/27936485/x-ray-crystal-structure-of-cytochrome-p450-monooxygenase-cyp101j2-from-sphingobium-yanoikuyae-strain-b2
#1
Birgit Unterweger, Nyssa Drinkwater, Priscilla Johanesen, Dena Lyras, Geoffrey J Dumsday, Sheena McGowan
The cytochrome P450 monooxygenases (P450s) catalyse a vast array of oxygenation reactions that can be useful in biocatalytic applications. CYP101J2 from Sphingobium yanoikuyae is a P450 that catalyses the hydroxylation of 1,8-cineole. Here we report the crystallisation and X-ray structure elucidation of recombinant CYP101J2 to 1.8 Å resolution. The CYP101J2 structure shows the canonical P450-fold and has an open conformation in the absence of substrate. Analysis of the structure revealed that CYP101J2, in the absence of substrate, forms a well-ordered substrate-binding channel that suggests a unique form of substrate guidance in comparison to other bacterial 1,8-cineole-hydroxylating P450 enzymes...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27936472/relaxant-effect-of-sodium-tanshinone-iia-sulphonate-on-mouse-tracheal-smooth-muscle
#2
Yi-Lin Zhang, Jia-Wen Xu, Xiong-Hao Wu, Peng-Xiao Chen, Fang Wei, Dong-Dong Gao, Xiao-Wen Chen, Yu-Li Luo, Yun-Xin Zhu, Jiehong Huang, Ya-Xia Tan, Lei Zhao, Wen-Liang Zhou
Sodium tanshinone IIA sulphonate, a water-soluble derivative of tanshinone IIA, has been proven to possess versatile biological properties, but its pharmacological effect on tracheal smooth muscle remains elusive. This paper presents a study on the relaxant effect and underlying mechanisms of sodium tanshinone IIA sulphonate on mouse tracheal smooth muscle. The relaxant effect of sodium tanshinone IIA sulphonate was evaluated in mouse tracheal rings using a mechanical recording system. Intracellular Ca(2+) concentration was measured in primary cultured tracheal smooth muscle cells using confocal imaging system...
December 9, 2016: Planta Medica
https://www.readbyqxmd.com/read/27936397/design-of-a-new-comprehensive-continuous-monitoring-system-for-environmental-radioactive-aerosol
#3
Hongkun Xu, Zihan Huang, Ge Wang, Changli Mu, Yin Yin
In order to comprehensive monitoring the radioactive isotopes from nuclear facilities, we developed a dual channel spectral monitoring instrument, and realized synchronous measurement for alpha, beta and gamma radionuclides. This article focuses on how to ensure its accuracy, stability and efficiency. First is the accuracy. In order to lower the interference of environmental and detector performance variation, the zero phase shift filter was designed to ensure the accuracy of characteristic peak position. Lorenz fitting algorithm was designed to reduce the effect of spectral low-energy tailing...
December 1, 2016: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27936219/combined-emd-sloreta-analysis-of-eeg-data-collected-during-a-contour-integration-task
#4
Karema Al-Subari, Saad Al-Baddai, Ana Maria Tomé, Gregor Volberg, Bernd Ludwig, Elmar W Lang
Lately, Ensemble Empirical Mode Decomposition (EEMD) techniques receive growing interest in biomedical data analysis. Event-Related Modes (ERMs) represent features extracted by an EEMD from electroencephalographic (EEG) recordings. We present a new approach for source localization of EEG data based on combining ERMs with inverse models. As the first step, 64 channel EEG recordings are pooled according to six brain areas and decomposed, by applying an EEMD, into their underlying ERMs. Then, based upon the problem at hand, the most closely related ERM, in terms of frequency and amplitude, is combined with inverse modeling techniques for source localization...
2016: PloS One
https://www.readbyqxmd.com/read/27936197/vascular-tone-regulation-induced-by-c-type-natriuretic-peptide-differences-in-endothelium-dependent-and-independent-mechanisms-involved-in-normotensive-and-spontaneously-hypertensive-rats
#5
Carolina Caniffi, Flavia M Cerniello, María N Gobetto, María L Sueiro, María A Costa, Cristina Arranz
Given that the role of C-type natriuretic peptide (CNP) in the regulation of vascular tone in hypertensive states is unclear, we hypothesized that impaired response of the nitric oxide system to CNP in spontaneously hypertensive rats (SHR) could affect vascular relaxation induced by the peptide in this model of hypertension, and that other endothelial systems or potassium channels opening could also be involved. We examined the effect of CNP on isolated SHR aortas, and the hindlimb vascular resistance (HVR) in response to CNP administration compared to normotensive rats...
2016: PloS One
https://www.readbyqxmd.com/read/27936151/osmoregulated-chloride-currents-in-hemocytes-from-mytilus-galloprovincialis
#6
Monica Bregante, Armando Carpaneto, Veronica Piazza, Francesca Sbrana, Massimo Vassalli, Marco Faimali, Franco Gambale
We investigated the biophysical properties of the transport mediated by ion channels in hemocytes from the hemolymph of the bivalve Mytilus galloprovincialis. Besides other transporters, mytilus hemocytes possess a specialized channel sensitive to the osmotic pressure with functional properties similar to those of other transport proteins present in vertebrates. As chloride fluxes may play an important role in the regulation of cell volume in case of modifications of the ionic composition of the external medium, we focused our attention on an inwardly-rectifying voltage-dependent, chloride-selective channel activated by negative membrane potentials and potentiated by the low osmolality of the external solution...
2016: PloS One
https://www.readbyqxmd.com/read/27936011/overload-control-for-signaling-congestion-of-machine-type-communications-in-3gpp-networks
#7
Zhaoming Lu, Qi Pan, Luhan Wang, Xiangming Wen
Because of the limited resources on radio access channels of third generation partnership projection (3GPP) network, one of the most challenging tasks posted by 3GPP cellular-based machine type communications (MTC) is congestion due to massive requests for connection to radio access network (RAN). In this paper, an overload control algorithm in 3GPP RAN is proposed, which proactively disperses the simultaneous access attempts in evenly distributed time window. Through periodic reservation strategy, massive access requests of MTC devices are dispersed in time, which reduces the probability of confliction of signaling...
2016: PloS One
https://www.readbyqxmd.com/read/27935988/effects-of-a-community-based-healthy-lifestyle-intervention-program-co-help-among-adults-with-prediabetes-in-a-developing-country-a-quasi-experimental-study
#8
Norliza Ibrahim, Foong Ming Moy, Intan Attikah Nur Awalludin, Zainudin Mohd Ali, Ikram Shah Ismail
BACKGROUND: The prevalence of type 2 diabetes among Malaysian adults has increased by more than two folds over the past two decades. Strategies to collaborate with the existing community partners may become a promising channel for wide-scale dissemination of diabetes prevention in the country. The objectives of this study were to determine the effects of community-based lifestyle interventions delivered to adults with prediabetes and their health-related quality of life as compared to the usual care group...
2016: PloS One
https://www.readbyqxmd.com/read/27935970/sanshool-on-the-fingertip-interferes-with-vibration-detection-in-a-rapidly-adapting-ra-tactile-channel
#9
Scinob Kuroki, Nobuhiro Hagura, Shin'ya Nishida, Patrick Haggard, Junji Watanabe
An Asian spice, Szechuan pepper (sanshool), is well known for the tingling sensation it induces on the mouth and on the lips. Electrophysiological studies have revealed that its active ingredient can induce firing of mechanoreceptor fibres that typically respond to mechanical vibration. Moreover, a human behavioral study has reported that the perceived frequency of sanshool-induced tingling matches with the preferred frequency range of the tactile rapidly adapting (RA) channel, suggesting the contribution of sanshool-induced RA channel firing to its unique perceptual experience...
2016: PloS One
https://www.readbyqxmd.com/read/27935900/-thromboangiitis-obliterans-buerger-s-disease-state-of-the-art
#10
D D Zerbino, E A Zimba, N N Bagry
Thromboangiitis obliterans (Buerger's disease) is systemic vasculitis with predominant development of thrombotic occlusions of small-to-medium diameter arteries of distal portions of both upper and lower limbs. A distinctive feature of Buerger's disease from other vasculitides is the involvement of the venous bed into the pathological process in the form of migrating thrombophlebitis. The disease is encountered more often in young adult males, predominantly tobacco smokers. The clinical pattern is presented by symptoms of increasing insufficiency of blood supply of tissues of extremities...
2016: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/27935893/-results-of-using-various-methods-of-autovenous-femoropopliteal-bypass-grafting-below-the-knee-joint-fissure
#11
B S Sukovatykh, L N Belikov, M B Sukovatykh, D V Sidorov, M A Inarkhov, E B Inokhodova
The objective of the study was to improve therapeutic outcomes in patients presenting with lower limb critical ischaemia by means of optimizing the technique of femoropopliteal bypass grafting. The authors analysed the results of comprehensive examination and treatment of a total of 90 patients presenting with critical lower limb ischaemia on the background of atherosclerotic lesions of arteries of the femoropopliteal and crural segments. Depending on the technology of performing femoropopliteal bypass grafting, the patients were subdivided into three groups each consisting of 30 subjects...
2016: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/27935846/guardians-in-a-stressful-world-the-opu-family-of-compatible-solute-transporters-from-bacillus-subtilis
#12
Tamara Hoffmann, Erhard Bremer
The development of a semi-permeable cytoplasmic membrane was a key event in the evolution of microbial proto-cells. As a result, changes in the external osmolarity will inevitably trigger water fluxes along the osmotic gradient. The ensuing osmotic stress has consequences for the magnitude of turgor and will negatively impact cell growth and integrity. No microorganism can actively pump water across the cytoplasmic membrane; hence, microorganisms have to actively adjust the osmotic potential of their cytoplasm to scale and direct water fluxes in order to prevent dehydration or rupture...
December 9, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/27935729/abnormal-neural-responses-to-feedback-in-depressed-adolescents
#13
Christian A Webb, Randy P Auerbach, Erin Bondy, Colin H Stanton, Dan Foti, Diego A Pizzagalli
Depression rates surge in adolescence, particularly among females. Recent findings suggest that depressed adolescents are characterized by hypersensitivity to negative outcomes and blunted responsiveness to rewards. However, our understanding of the pathophysiology and time course of these abnormalities remains limited. Due to their high temporal resolution, event-related potentials (ERPs) provide an ideal probe to investigate these processes. In the present study, healthy (n = 25) and depressed (n = 26) female adolescents (13-18 years) completed a gambling task during 128-channel ERP recording...
December 1, 2016: Journal of Abnormal Psychology
https://www.readbyqxmd.com/read/27935703/absorption-cross-sections-of-2-nitrophenol-in-the-295-400-nm-region-and-photolysis-of-2-nitrophenol-at-308-and-351-nm
#14
Manuvesh Sangwan, Lei Zhu
2-Nitrophenol is an important component of "brown carbon" in the atmosphere. Photolysis is its dominant gas phase removal process. We have determined the gas phase absorption cross sections of 2-nitrophenol in the 295-400 nm region by using cavity ring-down spectroscopy. 2-Nitrophenol exhibits a broad absorption band over the wavelength region studied, with the peak absorption located at 345 nm. Absorption cross section values range between (2.86 ± 0.18) × 10(-18) and (2.63 ± 0.31) × 10(-20) cm(2)/molecule over the 295-400 nm range...
December 9, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27935479/structural-basis-for-subtype-specific-inhibition-of-the-p2x7-receptor
#15
Akira Karasawa, Toshimitsu Kawate
The P2X7 receptor is a non-selective cation channel activated by extracellular adenosine triphosphate (ATP). Chronic activation of P2X7 underlies many health problems such as pathologic pain, yet we lack effective antagonists due to poorly understood mechanisms of inhibition. Here we present crystal structures of a mammalian P2X7 receptor complexed with five structurally-unrelated antagonists. Unexpectedly, these drugs all bind to an allosteric site distinct from the ATP-binding pocket in a groove formed between two neighboring subunits...
December 9, 2016: ELife
https://www.readbyqxmd.com/read/27935327/activation-induced-cell-death-aicd-of-human-melanoma-antigen-specific-tcr-engineered-cd8-t-cells-involves-jnk-bim-and-p53
#16
Arvind Chhabra, Bijay Mukherji, Deepika Batra
OBJECTIVES: Adoptive cancer immunotherapy (ACT) with transgenic T cell receptor (TCR) engineered (TCReng) anti-tumor T cells has produced encouraging results, however, efficacy of these approaches need improvement. Since premature activation induced cell death (AICD) of adoptively administered T cells could be a major impediment, we examined the mechanism(s) underlying AICD in TCReng CD8+ cytolytic T lymphocytes (CTL). METHODS: AICD in human tumor antigen specific MHC class I restricted TCR engineered CD8+ CTL was induced by exposing them to cognate peptide epitope...
December 9, 2016: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/27935309/pressurizing-field-effect-transistors-of-few-layer-mos2-in-a-diamond-anvil-cell
#17
Yabin Chen, Feng Ke, Penghong Ci, Changhyun Ko, Taegyun Park, Sahar Saremi, Huili Liu, Yeonbae Lee, Joonki Suh, Lane W Martin, Joel W Ager, Bin Chen, Junqiao Wu
Hydrostatic pressure applied using diamond anvil cells (DAC) has been widely explored to modulate physical properties of materials by tuning their lattice degree of freedom. Independently, electrical field is able to tune the electronic degree of freedom of functional materials via, for example, the field-effect transistor (FET) configuration. Combining these two orthogonal approaches would allow discovery of new physical properties and phases going beyond the known phase space. Such experiments are, however, technically challenging and have not been demonstrated...
December 9, 2016: Nano Letters
https://www.readbyqxmd.com/read/27935301/supramolecular-self-assembly-induced-adjustable-multiple-gating-states-of-nanofluidic-diodes
#18
Ruochen Fang, Huacheng Zhang, Liulin Yang, Huanting Wang, Ye Tian, Xi Zhang, Lei Jiang
Artificial nanochannels, inheriting smart gating functions of biological ion channels, promote the development of artificial functional nanofluidic devices for high-performance biosensing and electricity generation. However, gating states of the artificial nanochannels have been mainly realized through chemical modification of the channels with responsive molecules, and their gating states cannot be further regulated once the nanochannel is modified. In this work, we employed a new supramolecular layer-by-layer (LbL) self-assembly method to achieve reversible and adjustable multiple gating features in nanofluidic diodes...
December 9, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27935249/in-capillary-probing-of-quantum-dots-and-fluorescent-protein-self-assembly-and-displacement-using-f%C3%A3-rster-resonance-energy-transfer
#19
Jianhao Wang, Jie Fan, Jinchen Li, Li Liu, Jianpeng Wang, Pengju Jiang, Xiaoqian Liu, Lin Qiu
Herein, a Förster resonance energy transfer system was designed, which consisted of CdSe/ZnS quantum dots donor and mCherry fluorescent protein acceptor. The quantum dots and the mCherry proteins were conjugated to permit Förster resonance energy transfer. Capillary electrophoresis with fluorescence detection was used for the analyses for the described system. The quantum dots and mCherry were sequentially injected into the capillary, while the real-time fluorescence signal of donor and acceptor was simultaneously monitored by two channels with fixed wavelength detectors...
December 9, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27935216/modulation-of-diet-induced-mechanical-allodynia-by-metabolic-parameters-and-inflammation
#20
Michael A Cooper, Janelle M Ryals, Pau Yen Wu, Kellen D Wright, Katherine R Walter, Douglas E Wright
Dietary-associated diseases have increased tremendously in our current population, yet key molecular changes associated with high-fat diets that cause clinical prediabetes, obesity, hyperglycemia, and peripheral neuropathy remain unclear. This study examines molecular and metabolic aspects altered by voluntary exercise and a high-fat diet in the mouse dorsal root ganglion. Mice were examined for changes in mRNA and proteins encoding anti-inflammatory mediators, metabolic-associated molecules, and pain associated ion channels...
December 9, 2016: Journal of the Peripheral Nervous System: JPNS
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