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https://www.readbyqxmd.com/read/28448637/45s-rdna-external-transcribed-spacer-organization-reveals-new-phylogenetic-relationships-in-avena-genus
#1
Joana Rodrigues, Wanda Viegas, Manuela Silva
The genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid (AABB or AACC) and hexaploid species (AACCDD), constituting an interesting model for phylogenetic analysis. The aim of this work was to characterize 45S rDNA intergenic spacer (IGS) variability in distinct species representative of Avena genome diversity-A. strigosa (AA), A. ventricosa (CvCv), A. eriantha (CpCp), A. barbata (AABB), A. murphyi (AACC), A. sativa (AACCDD) and A. sterilis (AACCDD) through the assessment of the 5' external transcribed spacer (5'-ETS), a promising IGS region for phylogenetic studies poorly studied in Avena genus...
2017: PloS One
https://www.readbyqxmd.com/read/28423007/recombinants-from-the-crosses-between-amphidiploid-and-cultivated-peanut-arachis-hypogaea-for-pest-resistance-breeding-programs
#2
Ailton Ferreira de Paula, Naiana Barbosa Dinato, Bianca Baccili Zanotto Vigna, Alessandra Pereira Fávero
Peanut is a major oilseed crop worldwide. In the Brazilian peanut production, silvering thrips and red necked peanut worm are the most threatening pests. Resistant varieties are considered an alternative to pest control. Many wild diploid Arachis species have shown resistance to these pests, and these can be used in peanut breeding by obtaining hybrid of A and B genomes and subsequent polyploidization with colchicine, resulting in an AABB amphidiploid. This amphidiploid can be crossed with cultivated peanut (AABB) to provide genes of interest to the cultivar...
2017: PloS One
https://www.readbyqxmd.com/read/28370131/use-of-allogeneic-apheresis-stem-cell-products-as-an-interlaboratory-proficiency-challenge
#3
Laura Cooling, Kelly Roxbury, Sandra Hoffmann, Joan DeBusscher, Usha Kota, Steven Goldstein, Robertson Davenport
BACKGROUND: AABB Standards requires that laboratories participate in a proficiency test (PT) program for critical analytes. Institutions can purchase commercial PT materials; however, PT can also be performed through interlaboratory exchange. We investigated the utility of allogeneic hematopoietic progenitor cell apheresis (HPC-A) products as an interlaboratory PT challenge for total nucleated cell count (TNC) and CD34 assessment. STUDY DESIGN AND METHODS: Three-year retrospective and comparative review of unrelated allogeneic HPC-A products received by the University of Michigan between January 2011 and December 2013...
March 28, 2017: Transfusion
https://www.readbyqxmd.com/read/28250587/bread-wheat-progenitors-aegilops-tauschii-dd-genome-and-triticum-dicoccoides-aabb-genome-reveal-differential-antioxidative-response-under-water-stress
#4
Yadhu Suneja, Anil Kumar Gupta, Navtej Singh Bains
Antioxidant enzymes are known to play a significant role in scavenging reactive oxygen species and maintaining cellular homeostasis. Activity of four antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) was examined in the flag leaves of nine Aegilops tauschii and three Triticum dicoccoides accessions along with two bread wheat cultivars under irrigated and rain-fed conditions. These accessions were shortlisted from a larger set on the basis of field performance for a set of morpho-physiological traits...
January 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28243829/meiotic-pairing-as-an-indicator-of-genome-composition-in-polyploid-prairie-cordgrass-spartina-pectinata-link
#5
Jeffrey W Bishop, Sumin Kim, María B Villamil, D K Lee, A Lane Rayburn
The existence of neopolyploidy in prairie cordgrass (Spartina pectinata Link) has been documented. The neohexaploid was discovered coexisting with tetraploids in central Illinois, and has been reported to exhibit competitiveness in the natural environment. It is hypothesized that the natural tetraploid cytotype produced the hexaploid cytotype via production of unreduced gametes. Meiosis I chromosome pairing was observed in tetraploid (2n = 4x = 40), hexaploid (2n = 6x = 60), and octoploid (2n = 8x = 80) accessions and the percentage of meiotic abnormality was determined...
April 2017: Genetica
https://www.readbyqxmd.com/read/28043867/bont-ab-hybrid-maintains-similar-duration-of-paresis-as-bont-a-wild-type-in-murine-running-wheel-assay
#6
Anna Kutschenko, Marie-Christine Reinert, Nadja Krez, David Liebetanz, Andreas Rummel
The highly potent Botulinum neurotoxins (BoNT) are successful drugs to treat neuromuscular disorders. Efforts are being made to further reduce the injected BoNT dose and to lengthen the interval between treatments. Detailed knowledge of the BoNT structure-activity relationship (SAR) allows combining the best features of the different BoNT serotypes. Of all seven BoNT serotypes A-G, BoNT/A displays the highest potency despite low neuronal binding affinity, while BoNT/B exhibits much higher affinity. Recently, a new BoNT/AB hybrid (AABB) was constructed comprising the catalytic and translocation domain of BoNT/A and the 50kDa cell binding domain of BoNT/B...
December 30, 2016: Neurotoxicology
https://www.readbyqxmd.com/read/27958362/aggregation-and-gelation-of-aromatic-polyamides-with-parallel-and-anti-parallel-alignment-of-molecular-dipole-along-the-backbone
#7
Dan Zhu, Jing Shang, Xiaodong Ye, Jian Shen
The understanding of macromolecular structures and interactions is important but difficult, due to the facts that a macromolecules are of versatile conformations and aggregate states, which vary with environmental conditions and histories. In this work two polyamides with parallel or anti-parallel dipoles along the linear backbone, named as ABAB (parallel) and AABB (anti-parallel) have been studied. By using a combination of methods, the phase behaviors of the polymers during the aggregate and gelation, i.e...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870740/patient-blood-management-an-international-perspective
#8
Quentin Eichbaum, Michael Murphy, Yu Liu, Isaac Kajja, Ludhmila Abrahao Hajjar, Cees Th Smit Sibinga, Hua Shan
This article describes practices in patient blood management (PBM) in 4 countries on different continents that may provide insights for anesthesiologists and other physicians working in global settings. The article has its foundation in the proceedings of a session at the 2014 AABB annual meeting during which international experts from England, Uganda, China, and Brazil presented the programs and implementation strategies in PBM developed in their respective countries. To systematize the review and enhance the comparability between these countries on different continents, authors were requested to respond to the same set of 6 key questions with respect to their country's PBM program(s)...
December 2016: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/27867152/transmitting-characters-of-individual-e-chromosomes-of-thinopyrum-elongatum-in-triticum-turgidum-background
#9
Li Haifeng, Liu Huiping, Dai Yi, Huang Shuai, Zhang Jun, Gao Yong, Chen Jianmin
The transmission patterns of Thinopyrum elongatum chromosomes in the background of Triticum turgidum were investigated through cytogenetic and molecular marker analysis based on the F2 and F3 plants derived from a cross between Triticum trititrigia (AABBEE) and T. turgidum L. ssp. durum (AABB). An additional objective was to develop durum-Th. elongatum E chromosome addition lines. Among 218 F2 plants, individuals with 2n=28 accounted for 41.7%, those with 2n=29 accounted for 18.3%, and the remaining 40.0% had 2n=31-42...
November 20, 2016: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/27847512/evidence-of-genomic-exchanges-between-homeologous-chromosomes-in-a-cross-of-peanut-with-newly-synthetized-allotetraploid-hybrids
#10
Joel R Nguepjop, Hodo-Abalo Tossim, Joseph M Bell, Jean-François Rami, Shivali Sharma, Brigitte Courtois, Nalini Mallikarjuna, Djibril Sane, Daniel Fonceka
Cultivated peanut and synthetics are allotetraploids (2n = 4x = 40) with two homeologous sets of chromosomes. Meiosis in allotetraploid peanut is generally thought to show diploid-like behavior. However, a recent study pointed out the occurrence of recombination between homeologous chromosomes, especially when synthetic allotetraploids are used, challenging the view of disomic inheritance in peanut. In this study, we investigated the meiotic behavior of allotetraploid peanut using 380 SSR markers and 90 F2 progeny derived from the cross between Arachis hypogaea cv Fleur 11 (AABB) and ISATGR278-18 (AAKK), a synthetic allotetraploid that harbors a K-genome that was reported to pair with the cultivated B-genome during meiosis...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27820837/estimating-the-burden-of-chagas-disease-in-the-united-states
#11
Jennifer Manne-Goehler, Chukwuemeka A Umeh, Susan P Montgomery, Veronika J Wirtz
BACKGROUND: In recent years, there has been growing awareness of the significant burden of Chagas disease in the United States (US). However, epidemiological data on both prevalence and access to care for this disease are limited. The objective of this study is to provide an updated national estimate of Chagas disease prevalence, the first state-level estimates of cases of T. cruzi infection in the US and to analyze these estimates in the context of data on confirmed cases of infection in the US blood supply...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27794527/the-challenges-of-daratumumab-in-transfusion-medicine
#12
REVIEW
Michael F Dizon
The field of transfusion medicine has evolved rapidly in recent years, but the central principle of transfusion is still simple, namely, the antigen-antibody interaction. Daratumumab (DARA), a monoclonal antibody (MoAb), was developed to treat relapsed/refractory multiple myeloma (RRMM). DARA works by targeting the CD38 portion of malignant cells; however, this drug attaches to the red blood cell (RBC) reagents used in blood banks, further complicating the antibody identification work-up. The AABB (formerly known as the American Association of Blood Banks) has issued a memorandum on how blood banks can effectively address panreactivity caused by DARA...
February 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/27739152/red-blood-cell-transfusion-2016-clinical-practice-guidelines-from-aabb
#13
Aaron A R Tobian, Nancy M Heddle, Theresa L Wiegmann, Jeffrey L Carson
No abstract text is available yet for this article.
October 2016: Transfusion
https://www.readbyqxmd.com/read/27732721/clinical-practice-guidelines-from-the-aabb-red-blood-cell-transfusion-thresholds-and-storage
#14
Jeffrey L Carson, Gordon Guyatt, Nancy M Heddle, Brenda J Grossman, Claudia S Cohn, Mark K Fung, Terry Gernsheimer, John B Holcomb, Lewis J Kaplan, Louis M Katz, Nikki Peterson, Glenn Ramsey, Sunil V Rao, John D Roback, Aryeh Shander, Aaron A R Tobian
Importance: More than 100 million units of blood are collected worldwide each year, yet the indication for red blood cell (RBC) transfusion and the optimal length of RBC storage prior to transfusion are uncertain. Objective: To provide recommendations for the target hemoglobin level for RBC transfusion among hospitalized adult patients who are hemodynamically stable and the length of time RBCs should be stored prior to transfusion. Evidence Review: Reference librarians conducted a literature search for randomized clinical trials (RCTs) evaluating hemoglobin thresholds for RBC transfusion (1950-May 2016) and RBC storage duration (1948-May 2016) without language restrictions...
November 15, 2016: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/27732707/aabb-red-blood-cell-transfusion-guidelines-something-for-almost-everyone
#15
EDITORIAL
Mark H Yazer, Darrell J Triulzi
No abstract text is available yet for this article.
November 15, 2016: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/27708169/the-need-for-education-in-molecular-immunohematology-a-survey-of-specialists-in-blood-banking
#16
Stephanie N Kinney, Jayanna Slayten, Dan A Waxman
BACKGROUND: Within transfusion medicine, the education of molecular technologies lacks standardization. OBJECTIVE: To address this problem, we surveyed specialist in blood bank technology (SBBT) programs, immunohematology reference laboratories, and SBBT graduates to define its current state. METHODS: An anonymous online survey (SurveyMonkey) was emailed to the 15 American Association of Blood Banks (AABB) SBBT programs, 59 AABB IRLs, and 82 SBBT graduates...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27703178/rapid-genomic-changes-in-allopolyploids-of-carassius-auratus-red-var-%C3%A2-%C3%A2-%C3%A3-%C3%A2-megalobrama-amblycephala-%C3%A2
#17
Qinbo Qin, Zhengfa Lai, Liu Cao, Qiong Xiao, YuDe Wang, Shaojun Liu
To better understand genomic changes in the early generations after polyploidisation, we examined the chromosomal consequences of genomic merger in allotetraploid hybrids (4 nF1) (AABB, 4n = 148) of Carassius auratus red var. (RCC) (AA, 2n = 100) (♀) × Megalobrama amblycephala (BSB) (BB, 2n = 48) (♂). Complete loss of the paternal 5S rDNA sequence and the expected number of maternal chromosomal loci were found in 4 nF1, suggesting directional genomic changes occurred in the first generations after polyploidisation...
October 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27642142/a-supplement-to-transfusion-abstract-presentations-from-the-aabb-annual-meeting-orlando-fl-october-22-25-2016
#18
(no author information available yet)
No abstract text is available yet for this article.
September 2016: Transfusion
https://www.readbyqxmd.com/read/27611704/high-transferability-of-homoeolog-specific-markers-between-bread-wheat-and-newly-synthesized-hexaploid-wheat-lines
#19
Deying Zeng, Jiangtao Luo, Zenglin Li, Gang Chen, Lianquan Zhang, Shunzong Ning, Zhongwei Yuan, Youliang Zheng, Ming Hao, Dengcai Liu
Bread wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) has a complex allohexaploid genome, which makes it difficult to differentiate between the homoeologous sequences and assign them to the chromosome A, B, or D subgenomes. The chromosome-based draft genome sequence of the 'Chinese Spring' common wheat cultivar enables the large-scale development of polymerase chain reaction (PCR)-based markers specific for homoeologs. Based on high-confidence 'Chinese Spring' genes with known functions, we developed 183 putative homoeolog-specific markers for chromosomes 4B and 7B...
2016: PloS One
https://www.readbyqxmd.com/read/27455830/comparison-of-double-rbc-collection-by-blood-cell-separators
#20
Pimpun Kitpoka, Sarawan Chanthet, Viroje Chongkolwatana, Ampaiwan Chuansumrit, Mongkol Kunakorn
BACKGROUND: The problem of red blood cell (RBC) shortage occurs because of the expanding demand for blood utilization and the dfficulties in donor recruitment and retention. Resources can be maximized by using current technology to collect two units of RBC from the same donor during a single collection session. OBJECTIVE: To evaluate the performance, collection efficiency (CE), production cost, and donor satisfactions of two commercially available blood cell separators (BCS) for double dose red cell (DDRC) collection...
January 2016: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
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