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https://www.readbyqxmd.com/read/28239774/change-of-monascus-pigment-metabolism-and-secretion-in-different-extractive-fermentation-process
#1
Gong Chen, Rui Tang, Xiaofei Tian, Peng Qin, Zhenqiang Wu
Monascus pigments that were generally produced intracellularly from Monascus spp. are important natural colorants in food industry. In this study, change of pigment metabolism and secretion was investigated through fed-batch extractive fermentation and continuous extractive fermentation. The biomass, secreting rate of pigment and total pigment yield closely correlated with the activated time of extractive fermentation as well as the composition of feeding nutrients. Metal ions played a key role in both the cell growth and pigment metabolism...
February 26, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28239415/coupling-gene-regulatory-patterns-to-bioprocess-conditions-to-optimize-synthetic-metabolic-modules-for-improved-sesquiterpene-production-in-yeast
#2
Bingyin Peng, Manuel R Plan, Alexander Carpenter, Lars K Nielsen, Claudia E Vickers
BACKGROUND: Assembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both commodity chemicals (including biofuels) and high-value chemicals. Promoter-mediated transcriptional regulation coordinates the expression of the individual components of these heterologous pathways. Expression patterns vary during culture as conditions change, and this can influence yeast physiology and productivity in both positive and negative ways...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28238084/development-of-a-highly-efficient-oil-degumming-process-using-a-novel-phosphatidylinositol-specific-phospholipase-c-enzyme
#3
Sebastián Cerminati, Florencia Eberhardt, Claudia E Elena, Salvador Peirú, María E Castelli, Hugo G Menzella
Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and when combined with a second phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C, the three major phospholipids were completely hydrolyzed, providing an extra yield of oil greater than 2...
February 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28237597/comparison-of-immune-responses-in-calves-fed-heat-treated-or-unheated-colostrum
#4
S L Gelsinger, A J Heinrichs
Understanding the mechanisms that underlie neonatal immune function is important for appropriately treating and preventing disease. Cytokines provided in colostrum may affect immune development and function, but data describing cytokine absorption in calves and the effects of colostrum heat treatment on absorption are limited. The objectives of this experiment were to characterize immune responses in calves that received heat-treated (HT) or unheated (UH) colostrum (in terms of growth, rectal temperature, and blood cytokine and IgG concentrations) and to determine calves' ability to absorb IFNγ and IL1β from HT and UH colostrum...
February 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28236825/nitrogen-removal-in-a-staged-anaerobic-aerobic-sludge-digestion-system
#5
Jongmin Kim, John T Novak
  A mixture of primary and secondary municipal sludge was treated in the digestion system consisting of a first-stage mesophilic anaerobic and a second-stage aerobic digester. The study was to monitor solids and nitrogen removal efficiencies under different operational conditions in the aerobic digester.In the first phase, the aerobic digester was batch fed under continuous aeration. More solids and ammonia were removed as the aerobic retention time increased. About 90% or greater ammonia was removed at 3 days or longer aerobic retention times...
January 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28235991/process-development-of-itaconic-acid-production-by-a-natural-wild-type-strain-of-aspergillus-terreus-to-reach-industrially-relevant-final-titers
#6
Susan Krull, Antje Hevekerl, Anja Kuenz, Ulf Prüße
Itaconic acid is a promising organic acid and is commercially produced by submerged fermentation of Aspergillus terreus. The cultivation process of the sensitive filamentous fungus has been studied intensively since 1932, with respect to fermentation media components, oxygen supply, shearing rate, pH value, or culture method. Whereas increased final titers were achieved over the years, the productivity has so far remained quite low. In this study, the impact of the pH on the itaconic acid production was investigated in detail...
February 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28235990/enhanced-system-performance-by-dosing-ferroferric-oxide-during-the-anaerobic-treatment-of-tryptone-based-high-strength-wastewater
#7
Qidong Yin, Kai He, Aike Liu, Guangxue Wu
Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (ASBRs). One was the control reactor without amendment of Fe3O4, and the other was the Fe3O4-amended reactor. In an ASBR reaction cycle, the dosage of Fe3O4 increased the CH4 production rate by 12...
February 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28230321/disruption-of-genes-involved-in-corvet-complex-leads-to-enhanced-secretion-of-heterologous-carboxylesterase-only-in-protease-deficient-pichia-pastoris
#8
Lukas Marsalek, Clemens Gruber, Friedrich Altmann, Markus Aleschko, Diethard Mattanovich, Brigitte Gasser, Verena Puxbaum
The methylotrophic yeast Pichia pastoris (Komagataella spp.) is a popular microbial host for the production of recombinant proteins. Previous studies have shown that mis-sorting to the vacuole can be a bottleneck during production of recombinant secretory proteins in yeast, however, no information was available for P. pastoris. In this work we have therefore generated vps (vacuolar protein sorting) mutant strains disrupted in genes involved in the CORVET tethering complex at the early stages of endosomal sorting...
February 23, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28222737/dynamic-genome-scale-metabolic-modeling-of-the-yeast-pichia-pastoris
#9
Francisco Saitua, Paulina Torres, José Ricardo Pérez-Correa, Eduardo Agosin
BACKGROUND: Pichia pastoris shows physiological advantages in producing recombinant proteins, compared to other commonly used cell factories. This yeast is mostly grown in dynamic cultivation systems, where the cell's environment is continuously changing and many variables influence process productivity. In this context, a model capable of explaining and predicting cell behavior for the rational design of bioprocesses is highly desirable. Currently, there are five genome-scale metabolic reconstructions of P...
February 21, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/28214699/optimization-of-simultaneous-saccharification-and-fermentation-conditions-with-amphipathic-lignin-derivatives-for-concentrated-bioethanol-production
#10
Ningning Cheng, Keiichi Koda, Yutaka Tamai, Yoko Yamamoto, Taichi E Takasuka, Yasumitsu Uraki
Amphipathic lignin derivatives (A-LDs) prepared from the black liquor of soda pulping of Japanese cedar are strong accelerators for bioethanol production under a fed-batch simultaneous enzymatic saccharification and fermentation (SSF) process. To improve the bioethanol production concentration, conditions such as reaction temperature, stirring program, and A-LDs loadings were optimized in both small scale and large scale fed-batch SSF. The fed-batch SSF in the presence of 3.0g/L A-LDs at 38°C gave the maximum ethanol production and a high enzyme recovery rate...
February 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214439/different-cultivation-methods-to-acclimatise-ammonia-tolerant-methanogenic-consortia
#11
Hailin Tian, Ioannis A Fotidis, Enrico Mancini, Irini Angelidaki
Bioaugmentation with ammonia tolerant-methanogenic consortia was proposed as a solution to overcome ammonia inhibition during anaerobic digestion process recently. However, appropriate technology to generate ammonia tolerant methanogenic consortia is still lacking. In this study, three basic reactors (i.e. batch, fed-batch and continuous stirred-tank reactors (CSTR)) operated at mesophilic (37°C) and thermophilic (55°C) conditions were assessed, based on methane production efficiency, incubation time, TAN/FAN (total ammonium nitrogen/free ammonia nitrogen) levels and maximum methanogenic activity...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28213136/systematic-development-of-a-two-stage-fed-batch-process-for-lipid-accumulation-in-rhodotorula-glutinis
#12
Eric Lorenz, Dennis Runge, Anna-Maria Marbà-Ardébol, Maximilian Schmacht, Ulf Stahl, Martin Senz
The application of oleaginous yeast cells as feed supplement, for instance in aqua culture, can be a meaningful alternative for fish meal and oil additives. Therefore, a two-stage fed-batch process split into growth and lipogenesis phase was systematically developed to enrich the oleaginous yeast Rhodotorula glutinis Rh-00301 with high amounts of lipids at industrial relevant biomasses. Thereby, the different carbon sources glucose, sucrose and glycerol were investigated concerning their abilities to serve as a suited raw material for growth and/or lipid accumulation...
February 15, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28204983/application-of-acetyl-coa-acetyltransferase-atoad-in-escherichia-coli-to-increase-3-hydroxyvalerate-fraction-in-poly-3-hydroxybutyrate-co-3-hydroxyvalerate
#13
Jong-Min Jeon, Hyun-Joong Kim, Shashi Kant Bhatia, Changmin Sung, Hyung-Min Seo, Jung-Ho Kim, Hyung-Yeon Park, Dahye Lee, Christopher J Brigham, Yung-Hun Yang
Polyhydroxyalkanoate (PHA) is a family of biodegradable polymers, and incorporation of different monomers can alter its physical properties. To produce the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) containing a high level of 3-hydroxyvalerate (3HV) by altering acetyl-CoA pool levels, we overexpressed an acetyl-CoA acetyltransferase (atoAD) in an engineered E. coli strain, YH090, carrying PHA synthetic genes bktB, phaB, and phaC. It was found that, with introduction of atoAD and with propionate as a co-substrate, 3HV fraction in PHA was increased up to 7...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28197999/parallel-experimental-design-and-multivariate-analysis-provides-efficient-screening-of-cell-culture-media-supplements-to-improve-biosimilar-product-quality
#14
David Brühlmann, Michael Sokolov, Alessandro Butté, Markus Sauer, Jürgen Hemberger, Jonathan Souquet, Hervé Broly, Martin Jordan
Rational and high-throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design-of-experiment (DoE) of CHO fed-batch cultures in 96-deepwell plates to modulate monoclonal antibody (mAb) glycosylation using medium supplements. To reduce risk of losing valuable information in an intricate joint screening, 17 compounds were separated into five different groups, considering their mode of biological action...
February 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28197690/improvement-of-levan-production-in-bacillus-amyloliquefaciens-through-metabolic-optimization-of-regulatory-elements
#15
Yanyan Gu, Jiayi Zheng, Jun Feng, Mingfeng Cao, Weixia Gao, Yufen Quan, Yulei Dang, Yi Wang, Shufang Wang, Cunjiang Song
Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous promoters were evaluated for sacB expression, and the Pgrac promoter led to the highest level for both sacB transcription and levansucrase enzyme activity. The levan production in the corresponding recombinant strain ΔLP-pHTPgrac reached 30...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28191588/enhancing-fungal-production-of-galactaric-acid
#16
Dorothee Barth, Marilyn G Wiebe
Galactaric (mucic) acid is a symmetrical six carbon diacid which can be produced by oxidation of galactose with nitric acid, electrolytic oxidation of D-galacturonate or microbial conversion of D-galacturonate. Both salts and the free acid of galactarate have relatively low solubility, which may create challenges for a microbial host. Galactaric acid was most soluble at pH values around 4.7 in the presence of ammonium or sodium ions and less soluble in the presence of potassium ions. Solubility increased with increasing temperature...
February 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#17
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28188702/control-of-chitin-nanofiber-production-by-the-lipid-producing-diatom-cyclotella-sp-through-fed-batch-addition-of-dissolved-silicon-and-nitrate-in-a-bubble-column-photobioreactor
#18
Omar Chiriboga, Gregory L Rorrer
Diatoms are single-celled algae that make cell walls of nanopatterned biogenic silica called frustules through metabolic uptake of dissolved silicon and its templated condensation into biosilica. The centric marine diatom Cyclotella sp. also produces intracellular lipids and the valued co-product chitin, an N-acetyl glucosamine biopolymer that is extruded from selected frustule pores as pure nanofibers. The goal of this study was to develop a nutrient feeding strategy to control the production of chitin nanofibers from Cyclotella with the co-production of biofuel lipids...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28188696/applied-in-situ-product-recovery-in-abe-fermentation
#19
REVIEW
Victoria Outram, Carl-Axel Lalander, Jonathan G M Lee, E Timothy Davies, Adam P Harvey
The production of biobutanol is hindered by the product's toxicity to the bacteria, which limits the productivity of the process. In situ product recovery of butanol can improve the productivity by removing the source of inhibition. This paper reviews in situ product recovery techniques applied to the acetone butanol ethanol fermentation in a stirred tank reactor. Methods of in situ recovery include gas stripping, vacuum fermentation, pervaporation, liquid-liquid extraction, perstraction and adsorption, all of which have been investigated for the acetone, butanol and ethanol fermentation...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28186328/site-specific-monitoring-of-n-glycosylation-profiles-of-a-ctla4-fc-fusion-protein-from-the-secretory-pathway-to-the-extracellular-environment
#20
Karina Bora de Oliveira, David Spencer, Christopher Barton, Nitin Agarwal
Glycosylation often plays a key role in the safety and efficacy of therapeutic proteins to patients, thus underlying the need for consistent control of this important post-translational modification during biologics production. In this study, we profiled the site-specific evolution of N-glycans on a CTLA4-Fc-fusion protein, from the intracellular secretory pathway to the conditioned medium (CM) in fed-batch cell culture. For this, we developed an approach that combined sub-cellular fractionation with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses...
February 10, 2017: Biotechnology and Bioengineering
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