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https://www.readbyqxmd.com/read/28818103/enhancement-of-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-accumulation-in-arxula-adeninivorans-by-stabilization-of-production
#1
Mateusz Biernacki, Marek Marzec, Thomas Roick, Reinhard Pätz, Kim Baronian, Rüdiger Bode, Gotthard Kunze
BACKGROUND: In recent years the production of biobased biodegradable plastics has been of interest of researchers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for new applications. Commonly investigated are the polyhydroxyalkanoates (PHAs) poly(hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-V). The latter has the advantage of being tougher and less brittle. The production of these polymers in bacteria is well established but production in yeast may have some advantages, e...
August 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28818083/optimization-of-the-biotechnological-production-of-a-novel-class-of-anti-mrsa-antibiotics-from-chitinophaga-sancti
#2
Amelie Beckmann, Stephan Hüttel, Viktoria Schmitt, Rolf Müller, Marc Stadler
BACKGROUND: Recently, the discovery of the elansolids, a group of macrolides, was reported. The molecules show activity against methicillin-resistant Staphylococcus aureus as well as other gram-positive organisms. This fact renders those substances a promising starting point for future chemical development. The active atropisomers A1/A2 are formed by macrolactonization of the biosynthesis product A3 but are prone to ring opening and subsequent formation of several unwanted side products...
August 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28810215/continuous-operation-of-thermophilic-food-waste-digestion-with-side-stream-ammonia-stripping
#3
Wei Zhang, Sonia Heaven, Charles J Banks
Digesters fed on food waste (high nitrogen content) were operated successfully over an extended period using sidestream biogas stripping to control total ammonia nitrogen (TAN) below inhibitory concentrations. This is the first time biogas stripping has been used to achieve stable thermophilic operation with undiluted substrate of this type. Stripping columns operated batch-wise treated the equivalent of 1.7-4.1% of digester contents daily at pH >10 and 70°C, with no detrimental effect on digestion. TKN removal was 54%, with potential to recover 3...
August 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28805885/effects-of-steeped-or-fermented-distillers-dried-grains-with-solubles-on-growth-performance-in-weanling-pigs
#4
M Wiseman, B McBride, J Li, D Wey, J Zhu, C F M de Lange
Coproduct feeding value may be improved by enzyme and microbial inoculant treatment in liquid diets. Three experiments were conducted to assess growth performance in newly weaned 20-d-old pigs fed corn- and soybean meal-based diets with untreated, steeped, or partially fermented distillers dried grains with solubles (DDGS) from day of weaning. In Exp. 1, conventionally dry fed diets with untreated DDGS (DDGS) or without DDGS (Control) were fed. In Exp. 2, liquid diets (25% DM [75% moisture]) were fed with enzymes (β-glucanase and xylanase at 67...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28803126/fed-batch-production-of-vanillin-by-bacillus-aryabhattai-ba03
#5
Alicia Paz, David Outeiriño, Ricardo Pinheiro de Souza Oliveira, José Manuel Domínguez
Bacillus aryabhattai BA03, a strain isolated in our laboratory, has interesting properties related to the production of natural aromas and flavors. Specifically, we have found that it was able to produce vanillin from ferulic acid (FA). Furthermore, this strain produces high amounts of 4-vinylguaiacol in only 14h, this being the only intermediate metabolite observed in the process. FA is an inexpensive feedstock for the production of natural value-added compounds when extracted from lignocellulosic wastes. In this study, we optimized the operational conditions (temperature, pH and agitation), medium composition and bioconversion technology (batch or fed-batch) to produce vanillin...
August 9, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28803103/engineering-halomonas-bluephagenesis-td01-for-non-sterile-production-of-poly-3-hydroxybutyrate-co-4-hydroxybutyrate
#6
Xiangbin Chen, Jin Yin, Jianwen Ye, Haoqian Zhang, Xuemei Che, Yiming Ma, Mengyi Li, Lin-Ping Wu, Guo-Qiang Chen
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and γ-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70g/L cell dry weight (CDW) containing 63% P(3HB-co-12mol% 4HB) after 48h under non-sterile conditions...
July 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28803011/new-insights-on-the-metabolism-of-ricinoleic-acid-in-ruminants
#7
Susana P Alves, Cintia M Araujo, Rita C Queiroga, Marta S Madruga, Michelle O M Parente, Ariosvaldo N Medeiros, Rui J B Bessa
Dairy goats were fed a total mixed ration with or without the inclusion of castor oil (40 g/kg of DM) to study the metabolism of ricinoleic acid (12-OH,cis-9-18:1). Ten goats, at 39.7 ± 4.0 d in milk, were individually penned and allocated at random to the 2 experimental diets. Goats were manually milked twice a day. Milk fatty acids (FA) were analyzed as methyl esters and hydroxyl groups were derivatized in trimethylsilyl ethers. Apart from ricinoleic acid, 6 FA were only detected in the milk of the castor oil group...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28799944/experimental-power-of-laboratory-scale-results-and-transferability-to-full-scale-anaerobic-digestion
#8
Maximilian Lüdtke, Åke Nordberg, Christian Baresel
Anaerobic digestion is today internationally acknowledged as an environmentally sound process for energy and nutrient recovery from organic wastes, and it is the dominant sludge treatment technology in most countries' wastewater treatment plants. Laboratory- or pilot-scale experiments are commonly used as a first step to investigate the potential of new ideas or to confirm research hypothesis before confirmation in full-scale. The objectives of this study were to investigate transferability of methane yield assessments between laboratory- and full-scale, and to compare the influence of experimental uncertainties on experimental power in parallel continuous digester experiments for the two scales...
August 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28798913/assessing-nutrient-removal-kinetics-in-flushed-manure-using-chlorella-vulgaris-biomass-production
#9
Pramod Pandey, Jun Shi
The utilization of dairy wastewater for producing algal biomass is seen as a two-fold opportunity to treat wastewater and produce algae biomass, which can be potentially used for production of biofuels. In animal agriculture system, one of the major waste streams is dairy manure that contains high levels of nitrogen and phosphorus. Furthermore, it is produced abundantly in California's dairy industry, as well as many other parts of the world. We hypothesized that flushed manure, wastewater from a dairy farm, can be used as a potential feedstock after pretreatment to grow Chlorella vulgaris biomass and to reduce nutrients of manure...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28789914/biogas-production-from-steer-manures-in-vietnam-effects-of-feed-supplements-and-tannin-contents
#10
Cuong H Pham, Surinder Saggar, Cuong C Vu, Kevin R Tate, Thao T T Tran, Thi T Luu, Hanh T Ha, Huong L T Nguyen, Sven G Sommer
In developing countries, the simple biogas digesters installed underground without heating or stirring are seen as a 'green' technology to convert animal waste into biogas, a source of bio-energy. However, quantitative estimates of biogas production of manures from steers fed local feed diets at actual incubation temperatures have yet to be carried out. The aim of this study was to determine the methane (CH4) production potential of manures from steers in Vietnam offered traditional feed rations or supplemental diets...
August 5, 2017: Waste Management
https://www.readbyqxmd.com/read/28782952/biotransformation-of-chrysin-to-baicalein-selective-c6-hydroxylation-of-5-7-dihydroxyflavone-using-whole-yeast-cells-stably-expressing-human-cyp1a1-enzyme
#11
Ibidapo S Williams, Shifali Chib, Vijay K Nuthakki, Linda Gatchie, Prashant Joshi, Niteen A Narkhede, Ram A Vishwakarma, Sandip B Bharate, Saurabh Saran, Bhabatosh Chaudhuri
Naturally occurring polyphenolic compounds are of medicinal importance because of their unique antioxidant, anticancer, and chemopreventive properties. Baicalein, a naturally occurring polyhydroxy flavonoid possessing a diverse range of pharmacological activities, has been used in traditional medicines for treatment of various ailments. Apart from its isolation from natural sources, its synthesis has been reported via multistep chemical approaches. Here, we report a preparative-scale biotransformation, using whole yeast cells stably expressing human cytochrome P450 1A1 (CYP1A1) enzyme that allows regioselective C6-hydroxylation of 5,7-dihydroxyflavone (chrysin) to form 5,6,7-trihydroxyflavone (baicalein)...
August 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28779976/fate-of-triclosan-in-laboratory-scale-activated-sludge-reactors-effect-of-culture-acclimation
#12
Aybala Koc Orhon, Kemal Berk Orhon, Ulku Yetis, Filiz B Dilek
Triclosan (TCS); a widely used antimicrobial biocide, exists in several pharmaceutical and personal care products. Due to its wide usage, TCS is detected in wastewater at varying concentrations. Biological treatability of TCS and its effect on chemical oxygen demand (COD) removal efficiency were investigated running laboratory-scale pulse-fed sequencing batch reactors with acclimated and non-acclimated cultures. The culture was acclimatized to TCS by gradually increasing its concentration in the synthetic feed wastewater from 100 ng/L to 100 mg/L...
August 2, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28771492/production-of-an-anti-a%C3%AE-antibody-fragment-in-pichia-pastoris-and-in-vitro-and-in-vivo-validation-of-its-therapeutic-effect
#13
Laia Montoliu-Gaya, Gisela Esquerda-Canals, Silvia Bronsoms, Sandra Villegas
ScFv-h3D6 has been shown as an efficient therapy in the 3xTg-AD mouse model of Alzheimer's Disease. Because one of the major bottlenecks for the therapeutic uses of proteins produced in Escherichia coli is their potential contamination with endotoxins, LPS were extensively removed by a rather low-efficient, expensive, and time-consuming purification step. In addition, disulfide scrambling is favored in the reducing bacterial cytoplasm albeit the use of reductase deficient strains. To overcome these hurdles, as well as to improve the yield, the yeast Pichia pastoris, an endotoxin-free host system for recombinant protein production, has been used to produce scFv-h3D6, both in flask and in a fed-batch bioreactor...
2017: PloS One
https://www.readbyqxmd.com/read/28764105/biosynthesis-of-keto-acids-by-fed-batch-culture-of-yarrowia-lipolytica-wsh-z06
#14
Weizhu Zeng, Hailin Zhang, Sha Xu, Fang Fang, Jingwen Zhou
Both α-ketoglutarate (α-KG) and pyruvate (PYR) are important organic acids with promising applications in the food, pharmaceutical and chemical industries. During the production of α-KG by different microorganisms, PYR is always present as a by-product. Strategies have been applied to eliminate PYR accumulation since it can bring difficulties to the downstream separation process. However, modern separation technologies have already conquered this problem. Therefore, this study was aimed at simultaneously enhancing α-KG and PYR production by Yarrowia lipolytica WSH-Z06...
July 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28762009/a-fed-batch-strategy-to-produce-high-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate-terpolymer-yield-with-enhanced-mechanical-properties-in-bioreactor
#15
Nursolehah Abd Aziz, Kai-Hee Huong, Coswald Stephen Sipaut, A A Amirul
This study reports an efficient fed-batch strategy to improve poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) [P(3HB-co-3HV-co-4HB)] terpolymer production by Cupriavidus sp. USMAA2-4 with enhanced mechanical properties in bioreactor. The cultivations have been performed by combining oleic acid with γ-butyrolactone at different concentration ratios with 1-pentanol at a fixed concentration. The batch and fed-batch fermentations have resulted in P(3HB-co-3HV-co-4HB) with compositions of 9-35 mol% 3HV and 4-24 mol% 4HB monomers...
July 31, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28754360/glycosylation-flux-analysis-reveals-dynamic-changes-of-intracellular-glycosylation-flux-distribution-in-chinese-hamster-ovary-fed-batch-cultures
#16
Sandro Hutter, Thomas K Villiger, David Brühlmann, Matthieu Stettler, Hervé Broly, Miroslav Soos, Rudiyanto Gunawan
N-linked glycosylation of proteins has both functional and structural significance. Importantly, the glycan structure of a therapeutic protein influences its efficacy, pharmacokinetics, pharmacodynamics and immunogenicity. In this work, we developed glycosylation flux analysis (GFA) for predicting intracellular production and consumption rates (fluxes) of glycoforms, and applied this analysis to CHO fed-batch immunoglobulin G (IgG) production using two different media compositions, with and without additional manganese feeding...
July 25, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28751278/production-of-3-hydroxypropionic-acid-by-balancing-the-pathway-enzymes-using-synthetic-cassette-architecture
#17
Mugesh Sankaranarayanan, Ashok Somasundar, Eunhee Seol, Ashish Singh Chauhan, Seongjin Kwon, Gyoo Yeol Jung, Sunghoon Park
Biological 3-hydroxypropionic acid (3-HP) production from glycerol is a two-step reaction catalyzed by glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH). Recombinant strains developed for 3-HP production often suffer from the accumulation of a toxic intermediate, 3-hydroxypropionaldehyde (3-HPA). In order to avoid 3-HPA accumulation, balancing of the two enzymatic activities, in the present study, was attempted by employment of synthetic-regulatory cassettes comprising varying-strength promoters and bicistronic ribosome-binding sites (RBSs)...
July 24, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28748655/high-throughput-automated-microbial-bioreactor-system-used-for-clone-selection-and-rapid-scale-down-process-optimisation
#18
M Lourdes Velez-Suberbie, John Pj Betts, Kelly L Walker, Colin Robinson, Barney Zoro, Eli Keshavarz-Moore
High throughput automated fermentation systems have become a useful tool in early bioprocess development. In this study, we investigated a 24 x 15 mL single use microbioreactor system, ambr 15f, designed for microbial culture. We compared the fed-batch growth and production capabilities of this system for two Escherichia coli strains, BL21 (DE3) and MC4100, and two industrially relevant molecules, hGH and scFv. In addition, different carbon sources were tested using bolus, linear or exponential feeding strategies, showing the capacity of the ambr 15f system to handle automated feeding...
July 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28748636/compact-cell-settlers-for-perfusion-cultures-of-microbial-and-mammalian-cells
#19
Cassandra A Freeman, Premsingh S D Samuel, Dhinakar S Kompala
As microbial secretory expression systems have become well developed for microbial yeast cells, such as Saccharomyces cerevisiae and Pichia pastoris, it is advantageous to develop high cell density continuous perfusion cultures of microbial yeast cells to retain the live and productive yeast cells inside the perfusion bioreactor while removing the dead cells and cell debris along with the secreted product protein in the harvest stream. While the previously demonstrated inclined or lamellar settlers can be used for such perfusion bioreactors for microbial cells, the size and footprint requirements of such inefficiently scaled up devices can be quite large in comparison to the bioreactor size...
July 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28747009/pilot-scale-repeated-fed-batch-fermentation-processes-of-the-wine-yeast-dekkera-bruxellensis-for-mass-production-of-resveratrol-from-polygonum-cuspidatum
#20
Hsiao-Ping Kuo, Reuben Wang, Yi-Sheng Lin, Jinn-Tsyy Lai, Yi-Chen Lo, Shyue-Tsong Huang
Resveratrol has long been used as an ingredient in functional foods. Currently, Polygonum cuspidatum extract is the greatest natural source for resveratrol because of high concentrations of glycosidic-linked resveratrol. Thus, developing a cost-effective procedure to hydrolyze glucoside could substantially enhance resveratrol production from P. cuspidatum. This study selected Dekkera bruxellensis from several microorganisms based on its bioconversion and enzyme-specific activities. We demonstrated that the cells could be reused at least nine times while maintaining an average of 180...
July 12, 2017: Bioresource Technology
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