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https://www.readbyqxmd.com/read/28315440/optimized-production-and-characterization-of-a-detergent-stable-protease-from-lysinibacillus-fusiformis-c250r
#1
REVIEW
Sondes Mechri, Mouna Kriaa, Mouna Ben Elhoul Berrouina, Maroua Omrane Benmrad, Nadia Zaraî Jaouadi, Hatem Rekik, Khelifa Bouacem, Amel Bouanane-Darenfed, Alif Chebbi, Sami Sayadi, Mohamed Chamkha, Samir Bejar, Bassem Jaouadi
In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman design. Response surface methodology (RSM) was used to determine the optimum levels of the screened factors and their interaction. Under the optimized conditions, protease yield 3100U/mL was 4.5 folds higher than those obtained by the use of the initial conditions (680U/ml)...
March 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28303383/surgical-management-of-coronal-and-sagittal-imbalance-of-the-spine-without-pso-a-multicentric-cohort-study-on-compensated-adult-degenerative-deformities
#2
Alessandro Ramieri, Massimo Miscusi, Maurizio Domenicucci, Antonino Raco, Giuseppe Costanzo
PURPOSE: Sagittal imbalance of severe adult degenerative deformities requires surgical correction to improve pain, mobility and quality of life. Our aim was a harmonic and balanced spine, treating a series of adult degenerative thoracolumbar and lumbar kyphoscoliosis by a non posterior subtraction osteotomy technique. METHODS: We operated 22 painful thoracolumbar and lumbar compensated degenerative deformities by anterior (ALIF), extreme lateral (XLIF) and transforaminal (TLIF) interbody fusion and grade 2 osteotomy (SPO) to restore lumbar lordosis and mobilize the coronal curve...
March 16, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28277385/transforaminal-lumbar-interbody-fusion-versus-mini-open-anterior-lumbar-interbody-fusion-with-oblique-self-anchored-stand-alone-cages-for-the-treatment-of-lumbar-disc-herniation-a-retrospective-study-with-2-year-follow-up
#3
Guohua Lü, Lei Kuang, Bing Wang
STUDY DESIGN: Retrospective study OBJECTIVE.: To evaluate the clinical and radiological outcomes of mini-open ALIF (MO-ALIF) with self-anchored stand alone cages for the treatment of lumbar disc herniation in comparison with transforaminal lumbar interbody fusion (TLIF). SUMMARY OF BACKGROUND DATA: Currently, whether ALIF is superior to TLIF for the treatment of lumbar disc herniation remains controversial. METHODS: This study retrospectively reviewed 82 patients who underwent MO-ALIF with self-anchored standalone cages (n = 42) or TLIF (n = 40) for the treatment of lumbar disc herniation between April 2013 and October 2014...
March 8, 2017: Spine
https://www.readbyqxmd.com/read/28276661/anterior-lumbar-interbody-fusion-in-left-sided-inferior-vena-cava-and-right-sided-aortic-arch
#4
Kevin Phan, Bernard A M Fang, Monish M Maharaj, Andrew F Lennox, Ralph J Mobbs
Spinal fusion via anterior lumbar interbody fusion (ALIF) can offer symptomatic relief to patients that suffer severe low back pain, radiculopathy, and claudication. However, a detailed working knowledge of the thoracic, abdominal, and lumbar anatomy, particularly of the vasculature, is vital. We report the case of a 68-year-old man who presented with radiculopathy and progressively worsening low back pain despite 9 months of unsuccessful conservative therapy and pain management. Preoperative computed tomography and magnetic resonance imaging revealed a rare anatomical variation, with an anomalous left-sided inferior vena cava and anomalous aorta...
March 9, 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28247079/two-piece-alif-cage-optimizes-the-bone-implant-interface-in-a-360%C3%A2-setting
#5
Hans-Joachim Wilke, David Volkheimer, Bruce Robie, Finn B Christensen
INTRODUCTION: Clinical research has documented that cage subsidence and the loss of balance correction is a significant complication related to spinal fusion. Subsidence is a multifactorial complication, where implant design is one important element. The aim of the study is to compare the rigidity and bone-implant relative motion of segments treated with either a conventional one-piece ALIF cage versus a two-piece ALIF cage, which adapts in situ and permits 7°-21° of lordosis. METHODS: Seven lumbosacral (L3-S1) human cadaver specimens were tested in a universal spine tester in the intact condition, the specimens instrumented with a two-piece ALIF cage (Statur(®)-L, FBC Device, Denmark) and a one-piece ALIF cage (Pezo™-A, Ulrich GmbH & Co...
February 28, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28156000/rhamnolipids-from-pseudomonas-aeruginosa-strain-w10-as-antibiofilm-antibiofouling-products-for-metal-protection
#6
Alif Chebbi, Mohamed Elshikh, Farazul Haque, Syed Ahmed, Sara Dobbin, Roger Marchant, Sami Sayadi, Mohamed Chamkha, Ibrahim M Banat
Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L(-1) and a critical micelle concentration (CMC) of 80 mg L(-1) . P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28067466/anterior-lumbar-interbody-fusion-integrated-screw-cages-intrinsic-load-generation-subsidence-and-torsional-stability
#7
Yusuf Assem, Matthew H Pelletier, Ralph J Mobbs, Kevin Phan, William R Walsh
OBJECTIVE: To perform a repeatable idealized in vitro model to evaluate the effects of key design features and integrated screw fixation on unloaded surface engagement, subsidence, and torsional stability. METHODS: We evaluated four different stand-alone anterior lumbar interbody fusion (ALIF) cages with two, three, and four screw designs. Polyurethane (saw-bone) foam blocks were used to simulate the vertebral bone. Fuji Film was used to measure the contact footprint, average pressure, and load generated by fixating the cages with screws...
January 9, 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#8
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28050828/tissue-culture-for-xylem-differentiation-with-arabidopsis-leaves
#9
Masato Saito, Alif Meem Nurani, Yuki Kondo, Hiroo Fukuda
Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28043848/surgical-treatment-of-degenerative-spondylolisthesis
#10
REVIEW
P Guigui, E Ferrero
Degenerative spondylolisthesis is a common pathology, often causing lumbar canal stenosis. There is, however, no strong consensus regarding the various medical and surgical treatments available. Surgery is indicated mainly for perceived functional impairment; when the indication is accepted, several questions determine the choice of surgical strategy. Improvement in neurological symptoms is one of the main treatment objectives. For this, it is useful to perform radicular decompression. Some authors recommend indirect decompression by interbody fusion (ALIF, TLIF, XLIF), others by means of an interspinous spacer but the most frequent technique is direct posterior decompression...
December 30, 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28038691/strain-in-posterior-instrumentation-resulted-by-different-combinations-of-posterior-and-anterior-devices-for-long-spine-fusion-constructs
#11
Christopher J Kleck, Damian Illing, Emily M Lindley, Andriy Noshchenko, Vikas V Patel, Cameron Barton, Todd Baldini, Christopher M J Cain, Evalina L Burger
STUDY DESIGN: Clinically related experimental study. OBJECTIVE: Evaluation of strain in posterior low lumbar and spinopelvic instrumentation for multilevel fusion resulting from the impact of such mechanical factors as physiologic motion, different combinations of posterior and anterior instrumentation, and different techniques of interbody device implantation. SUMMARY OF BACKGROUND DATA: Currently different combinations of posterior and anterior instrumentation as well as surgical techniques are used for multilevel lumbar fusion...
January 2017: Spine Deformity
https://www.readbyqxmd.com/read/28024374/symmetry-driven-band-gap-engineering-in-hydrogen-functionalized-graphene
#12
Jakob Holm Jørgensen, Antonija Grubišić Čabo, Richard Balog, Line Kyhl, Michael N Groves, Andrew Martin Cassidy, Albert Bruix, Marco Bianchi, Maciej Dendzik, Mohammad Alif Arman, Lutz Lammich, José Ignacio Pascual, Jan Knudsen, Bjørk Hammer, Philip Hofmann, Liv Hornekaer
Band gap engineering in hydrogen functionalized graphene is demonstrated by changing the symmetry of the functionalization structures. Small differences in hydrogen adsorbate binding energies on graphene on Ir(111) allow tailoring of highly periodic functionalization structures favoring one distinct region of the moiré supercell. Scanning tunneling microscopy and X-ray photoelectron spectroscopy measurements show that a highly periodic hydrogen functionalized graphene sheet can thus be prepared by controlling the sample temperature (Ts) during hydrogen functionalization...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/28009577/the-species-beamline-at-the-max-iv-laboratory-a%C3%A2-facility-for-soft-x-ray-rixs-and-apxps
#13
Samuli Urpelainen, Conny Såthe, Walan Grizolli, Marcus Agåker, Ashley R Head, Margit Andersson, Shih Wen Huang, Brian N Jensen, Erik Wallén, Hamed Tarawneh, Rami Sankari, Ralf Nyholm, Mirjam Lindberg, Peter Sjöblom, Niclas Johansson, Benjamin N Reinecke, M Alif Arman, Lindsay R Merte, Jan Knudsen, Joachim Schnadt, Jesper N Andersen, Franz Hennies
SPECIES is an undulator-based soft X-ray beamline that replaced the old I511 beamline at the MAX II storage ring. SPECIES is aimed at high-resolution ambient-pressure X-ray photoelectron spectroscopy (APXPS), near-edge X-ray absorption fine-structure (NEXAFS), X-ray emission spectroscopy (XES) and resonant inelastic X-ray scattering (RIXS) experiments. The beamline has two branches that use a common elliptically polarizing undulator and monochromator. The beam is switched between the two branches by changing the focusing optics after the monochromator...
January 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/27994777/mini-open-anterior-lumbar-interbody-fusion-combined-with-lateral-lumbar-interbody-fusion-in-corrective-surgery-for-adult-spinal-deformity
#14
Chong-Suh Lee, Se-Jun Park, Sung-Soo Chung, Jun-Young Lee, Tae-Hoon Yum, Seong-Kee Shin
STUDY DESIGN: Prospective observational study. PURPOSE: To introduce the techniques and present the surgical outcomes of mini-open anterior lumbar interbody fusion (ALIF) at the most caudal segments of the spine combined with lateral lumbar interbody fusion (LLIF) for the correction of adult spinal deformity. OVERVIEW OF LITERATURE: Although LLIF is increasingly used to correct adult spinal deformity, the correction of sagittal plane deformity with LLIF alone is reportedly suboptimal...
December 2016: Asian Spine Journal
https://www.readbyqxmd.com/read/27991591/anode-potential-influences-the-structure-and-function-of-anodic-electrode-and-electrolyte-associated-microbiomes
#15
Paul G Dennis, Bernardino Virdis, Inka Vanwonterghem, Alif Hassan, Phil Hugenholtz, Gene W Tyson, Korneel Rabaey
Three bioelectrochemical systems were operated with set anode potentials of +300 mV, +550 mV and +800 mV vs. Standard Hydrogen Electrode (SHE) to test the hypothesis that anode potential influences microbial diversity and is positively associated with microbial biomass and activity. Bacterial and archaeal diversity was characterized using 16 S rRNA gene amplicon sequencing, and biofilm thickness was measured as a proxy for biomass. Current production and substrate utilization patterns were used as measures of microbial activity and the mid-point potentials of putative terminal oxidases were assessed using cyclic voltammetry...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27989724/frailty-is-associated-with-morbidity-in-adults-undergoing-elective-anterior-lumbar-interbody-fusion-alif-surgery
#16
Kevin Phan, Jun S Kim, Nathan J Lee, Sulaiman Somani, John Di Capua, Parth Kothari, Dante Leven, Samuel K Cho
BACKGROUND CONTEXT: Prior studies have suggested no significant differences in functional status and postoperative complications of elderly versus nonelderly patients undergoing posterior lumbar interbody fusion; however, similar studies have not been comprehensively investigated in the setting of anterior lumbar interbody fusion (ALIF). PURPOSE: The objective was to quantify the ability of the modified Frailty Index (mFI) to predict postoperative events in patients undergoing ALIF...
October 27, 2016: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/27884744/technical-description-of-oblique-lateral-interbody-fusion-at-l1-l5-olif25-and-at-l5-s1-olif51-and-evaluation-of-complication-and-fusion-rates
#17
Kamal R M Woods, James B Billys, Richard A Hynes
BACKGROUND: The OLIF procedure is aimed at mitigating some of the challenges seen with traditional ALIF and transpsoas LLIF and allows for interbody fusion at L1-S1. PURPOSE: To describe the OLIF technique and assess the complication and fusion rates. STUDY DESIGN: A retrospective cohort study. PATIENT SAMPLE: 137 patients who underwent the oblique lateral interbody fusion (OLIF) procedure. OUTCOME MEASURES: Adverse events within 6 months of surgery: Infection, symptomatic pseudarthrosis, hardware failure, vascular injury, perioperative blood transfusion, ureteral injury, bowel injury, renal injury, prolonged postoperative ileus (greater than 3 days), incisional hernia, pseudohernia, reoperation, neurological deficits (weakness, numbness, paresthesia), hip flexion pain, retrograde ejaculation, sympathectomy affecting lower extremities, deep vein thrombosis, pulmonary embolism, myocardial infarction, pneumonia, and cerebrovascular accident...
November 21, 2016: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/27853663/impact-of-surgical-approach-on-clinical-outcomes-in-the-treatment-of-lumbar-pseudarthrosis
#18
R Kirk Owens, Mladen Djurasovic, Charles H Crawford, Steven D Glassman, John R Dimar, Leah Y Carreon
Study Design Retrospective comparative cohort. Objective Pseudarthrosis following fusion for degenerative lumbar spine pathologies remains a substantial problem. Current data shows that patients who develop a pseudarthrosis have suboptimal outcomes. This study evaluates if treatment of pseudarthrosis can be affected by surgical approach. Methods Medical records of 63 female and 65 male patients (mean age 50.37) who were treated for nonunion following lumbar fusion were reviewed. Sixty patients underwent posterolateral fusion (PSF), 18 underwent PSF with transforaminal interbody fusion (TLIF), 32 underwent anterior and posterior spinal fusion (AP), and 24 underwent anterior lumbar interbody fusion (ALIF)...
December 2016: Global Spine Journal
https://www.readbyqxmd.com/read/27847576/surgical-outcomes-of-post-fusion-lumbar-flatback-deformity-with-sagittal-imbalance
#19
Jin Seong Kim, Sung Min Kim
OBJECTIVES: To review surgical results of post-fusion lumbar flatback treated with pedicle subtraction osteotomy (PSO) or Smith-Petersen osteotomies (SPOs). METHODS: Twenty-eight patients underwent osteotomies. Radiological outcomes by sagittal vertical axis (SVA), and pelvic tilt (PT), T1 pelvic angle (T1PA), and pelvic incidence (PI)-lumbar lordosis (LL) at preoperative, postoperative 1 month, and final were evaluated. Oswestry Disability Index (ODI), visual analog scale (VAS) score of back pain/leg pain, and Scoliosis Research Society-22 score (SRS-22r) were analyzed and compared...
November 2016: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/27843688/extreme-lateral-lumbar-interbody-fusion-do-the-cons-outweigh-the-pros
#20
Nancy E Epstein
BACKGROUND: Major factors prompted the development of minimally invasive (MIS) extreme lateral interbody fusion (XLIF; NuVasive Inc., San Diego, CA, USE) for the thoracic/lumbar spine. These include providing interbody stabilization and indirect neural decompression while avoiding major visceral/vessel injury as seen with anterior lumbar interbody fusion (ALIF), and to avert trauma to paraspinal muscles/facet joints found with transforaminal lumbar interbody fusion (TLIF), posterior lumbar interbody fusion (PLIF), and posterior-lateral fusion techniques (PLF)...
2016: Surgical Neurology International
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