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https://www.readbyqxmd.com/read/27922666/antimicrobial-peptide-ll-37-promotes-yb-1-expression-and-the-viability-migration-and-invasion-of-malignant-melanoma-cells
#1
Jinjing Jia, Yan Zheng, Wei Wang, Yongping Shao, Zhengxiao Li, Qiong Wang, Yuan Wang, Huling Yan
The cathelicidin antimicrobial peptide, LL-37, is a multifunctional peptide with a broad spectrum of antimicrobial activities, such as chemotaxis and neutralizing endotoxins. Previous studies have demonstrated that it LL‑37 serves a functional role in the development of numerous types of cancer including ovarian, breast, prostate and lung cancer. However, its role in the development of malignant melanoma (MM) remains unclear. To determine the role of LL‑37 and the potential interaction with Y-box binding protein 1 (YB‑1) in MM, RNA interference, western blot, reverse transcription-quantitative polymerase chain reaction, MTT and Transwell assays were performed...
December 2, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27911878/y-box-binding-protein-1-promotes-hepatocellular-carcinomainitiating-cell-progression-and-tumorigenesis-via-wnt-%C3%AE-catenin-pathway
#2
Hsiao-Mei Chao, Hong-Xuan Huang, Po-Hsiang Chang, Kuo-Chang Tseng, Atsushi Miyajima, Edward Chern
Y-box binding protein-1 (YB-1) is a pleiotropic molecule that binds DNA to regulate genes on a transcriptional level in the nucleus and binds RNA to modulate gene translation in the cytoplasm. In our previous studies, YB-1 was also characterized as a fetal hepatic protein that regulates the maturation of hepatocytes and is upregulated during liver regeneration. Moreover, YB-1 has been shown to be expressed in human hepatocellular carcinoma (HCC). However, the role of YB-1 in HCC remains unclear. Here, we aimed to characterize the role of YB-1 in HCC...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27894167/salinomycin-reduces-stemness-and-induces-apoptosis-on-human-ovarian-cancer-stem-cell
#3
Hyun Gyo Lee, So Jin Shin, Hye Won Chung, Sang Hoon Kwon, Soon Do Cha, Jin Eui Lee, Chi Heum Cho
OBJECTIVE: Cancer stem cells (CSCs) represent a subpopulation of undifferentiated tumorigenic cells thought to be responsible for tumor initiation, maintenance, drug resistance, and metastasis. The role of CSCs in drug resistance and relapse of cancers could significantly affect outcomes of ovarian cancer patient. Therefore, therapies that target CSCs could be a promising approach for ovarian cancer treatment. The antibiotic salinomycin has recently been shown to deplete CSCs. In this study, we evaluated the effect of salinomycin on ovarian cancer stem cells (OCSCs), both alone and in combination with paclitaxel (PTX)...
November 14, 2016: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/27889204/tauroursodeoxycholic-acid-attenuates-angiotensin-ii-induced-abdominal-aortic-aneurysm-formation-in-apolipoprotein-e-deficient-mice-by-inhibiting-endoplasmic-reticulum-stress
#4
Y Qin, Y Wang, O Liu, L Jia, W Fang, J Du, Y Wei
OBJECTIVE/BACKGROUND: Abdominal aortic aneurysm (AAA) is characterised by the infiltration of smooth muscle cell (SMC) apoptosis, inflammatory cells, neovascularisation, and degradation of the extracellular matrix. Previous work has shown that endoplasmic reticulum (ER) stress and SMC apoptosis were increased both in a mouse model and human thoracic aortic aneurysm. However, whether the ER stress is activated in AAA formation and whether suppressing ER stress attenuates AAA is unknown...
November 24, 2016: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/27884304/a-remedy-for-kidney-disease-successfully-alters-the-cold-shock-protein-response-during%C3%A2-inflammation
#5
Sabine Brandt, Peter R Mertens
Kidneys undergoing acute inflammatory responses are characterized by cell infiltration and a cytokinergic milieu. The hazard resides in the perpetuation of inflammation and ensuing fibrosis. In this issue of Kidney International, Wang et al.(4) identify the cold shock Y-box binding protein-1 as the key orchestrator of cell infiltration in experimental tubulointerstitial nephritis following ureteral obstruction. Intriguingly, a small molecule previously designed to interfere with Y-box binding protein-1 interactions mediates an anti-inflammatory response and halts fibrogenesis...
December 2016: Kidney International
https://www.readbyqxmd.com/read/27856639/the-atypical-dual-specificity-phosphatase-hyvh1-associates-with-multiple-ribonucleoprotein-particles
#6
Qiudi Geng, Besa Xhabija, Colleen Knuckle, Christopher A Bonham, Panayiotis O Vacratsis
Human YVH1 (hYVH1), also known as dual specificity phosphatase 12 (DUSP12) is a poorly characterized atypical dual specificity phosphatase widely conserved throughout evolution. Recent findings have demonstrated that hYVH1 expression affects cellular DNA content and is a novel cell survival phosphatase preventing both thermal and oxidative stress induced cell death, while studies in yeast have established YVH1 as a novel 60S ribosome biogenesis factor. In this study, we have isolated novel hYVH1 associating proteins from human U2OS osteosarcoma cells using affinity chromatography coupled to mass spectrometry employing ion mobility separation...
November 17, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27845261/ddit3-suppresses-the-differentiation-of-mouse-chondroprogenitor-cells
#7
Miao Yu, Si-Qi Yi, Yan-Ru Wu, Hua-Ling Sun, Fang-Fang Song, Jia-Wei Wang
Endochondral ossification is an essential skeletal development process which is strongly linked to chondrocyte differentiation. DNA damage-inducible transcript 3 (Ddit3), a member of the CCAAT/enhancer-binding protein family of transcription factors, is highly expressed in the cartilage plate. However, the role of DNA damage-inducible transcript 3 in chondrocyte differentiation remains to be investigated. Immunofluorescent staining was used to detect Ddit3 expression in the mouse growth plate and in the mouse chondroprogenitor cell line ATDC5...
December 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27803124/rice-aleurone-layer-specific-osnf-yb1-regulates-grain-filling-and-endosperm-development-by-interacting-with-an-erf-transcription-factor
#8
Jing-Jing Xu, Xiao-Fan Zhang, Hong-Wei Xue
Grain yield and quality of rice mainly depend on grain filling and endosperm development. Here we report that a rice NUCLEAR FACTOR Y (NF-Y) transcription factor, OsNF-YB1, is specifically expressed in the aleurone layer of developing endosperm and regulates grain filling and endosperm development. Knockdown of OsNF-YB1 expression by RNAi significantly retarded grain filling, leading to small grains with chalky endosperm as well as altered starch quality. Whereas OsNF-YB1 shows subcellular localization in both the cytosol and the nucleus in roots, it was specifically targeted to the nucleus of aleurone layer cells, which was facilitated by interacting with OsNF-YC proteins preferentially expressed in the aleurone layer...
November 1, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27798850/identification-and-molecular-characterization-of-cellular-factors-required-for-glucocorticoid-receptor-mediated-mrna-decay
#9
Ok Hyun Park, Joori Park, Mira Yu, Hyoung-Tae An, Jesang Ko, Yoon Ki Kim
Glucocorticoid (GC) receptor (GR) has been shown recently to bind a subset of mRNAs and elicit rapid mRNA degradation. However, the molecular details of GR-mediated mRNA decay (GMD) remain unclear. Here, we demonstrate that GMD triggers rapid degradation of target mRNAs in a translation-independent and exon junction complex-independent manner, confirming that GMD is mechanistically distinct from nonsense-mediated mRNA decay (NMD). Efficient GMD requires PNRC2 (proline-rich nuclear receptor coregulatory protein 2) binding, helicase ability, and ATM-mediated phosphorylation of UPF1 (upstream frameshift 1)...
September 15, 2016: Genes & Development
https://www.readbyqxmd.com/read/27794479/transportin-1-dependent-yb-1-nuclear-import
#10
Daria A Mordovkina, Ekaterina R Kim, Ilya A Buldakov, Alexey V Sorokin, Irina A Eliseeva, Dmitry N Lyabin, Lev P Ovchinnikov
The DNA/RNA-binding protein YB-1 (Y-box binding protein 1) performs multiple functions both in the cytoplasm and the nucleus of the cell. Generally localized to the cytoplasm, under certain conditions YB-1 is translocated to the nucleus. Here we report for the first time a transport factor that mediates YB-1 nuclear import - transportin-1. The YB-1/transportin-1 complex can be isolated from HeLa cell extract. Nuclear import of YB-1 and its truncated form YB-1 (1-219) in in vitro transport assay was diminished in the presence of a competitor substrate and ceased in the presence of transportin-1 inhibitor M9M...
October 26, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27787879/impact-of-hmgb1-on-melanocytic-survival-and-its-involvement-in-the-pathogenesis-of-vitiligo
#11
J Y Kim, E J Lee, J Seo, S H Oh
BACKGROUND: Vitiligo is attributable to loss of functional melanocytes and is the most common acquired depigmenting disorder. Oxidative stress and intense UV irradiation are known to aggravate this condition. The non-histone high mobility group box 1 (HMGB1) DNA binding protein is a physiologic activator of immune responses, cellular proliferation, and cell death. Although implicated in the pathogenesis of autoimmune diseases and cutaneous disorders, the precise role of HMGB1 in melanocytes has yet to be studied...
October 27, 2016: British Journal of Dermatology
https://www.readbyqxmd.com/read/27782803/co-activator-candidate-interactions-for-orphan-nuclear-receptor-nr2e1
#12
Ximena Corso-Díaz, Charles N de Leeuw, Vivian Alonso, Diana Melchers, Bibiana K Y Wong, René Houtman, Elizabeth M Simpson
BACKGROUND: NR2E1 (Tlx) is an orphan nuclear receptor that regulates the maintenance and self-renewal of neural stem cells, and promotes tumourigenesis. Nr2e1-null mice exhibit reduced cortical and limbic structures and pronounced retinal dystrophy. NR2E1 functions mainly as a repressor of gene transcription in association with the co-repressors atrophin-1, LSD1, HDAC and BCL11A. Recent evidence suggests that NR2E1 also acts as an activator of gene transcription. However, co-activator complexes that interact with NR2E1 have not yet been identified...
October 26, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27774594/cellular-source-and-proinflammatory-roles-of-high-mobility-group-box-1-in-surgically-injured-rat-vocal-folds
#13
Nicole Y K Li-Jessen, Michael Powell, Ae-Jin Choi, Byung-Joo Lee, Susan L Thibeault
OBJECTIVES/HYPOTHESIS: High-mobility group box 1 (HMGB1) is a chromatin-binding protein located in the cell nucleus. Following injury, immunocompetent cells secrete HMGB1 to the extracellular milieu under the stimulation of proinflammatory cytokines. Extracellular HMGB1 acts a danger signal that instigates the innate immunity and tissue repair. We previously reported HMGB1 in the vocal fold extracellular compartment between day 3 and day 7 following surgical injury. In this study, we further investigated the cell source of HMGB1 and the relationship of proinflammatory cytokine expression and HMGB1 translocation in wounded vocal folds...
October 24, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27750031/modulation-of-topoisomerase-ii%C3%AE-expression-and-chemosensitivity-through-targeted-inhibition-of-nf-y-dna-binding-by-a-diamino-p-anisyl-benzimidazole-hx-polyamide
#14
Luke Pett, Konstantinos Kiakos, Vijay Satam, Pravin Patil, Sarah Laughlin-Toth, Matthew Gregory, Michael Bowerman, Kevin Olson, Mia Savagian, Megan Lee, Moses Lee, W David Wilson, Daniel Hochhauser, John A Hartley
BACKGROUND: Sequence specific polyamide HxIP 1, targeted to the inverted CCAAT Box 2 (ICB2) on the topoisomerase IIα (topo IIα) promoter can inhibit NF-Y binding, re-induce gene expression and increase sensitivity to etoposide. To enhance biological activity, diamino-containing derivatives (HxI*P 2 and HxIP* 3) were synthesised incorporating an alkyl amino group at the N1-heterocyclic position of the imidazole/pyrrole. METHODS: DNase I footprinting was used to evaluate DNA binding of the diamino Hx-polyamides, and their ability to disrupt the NF-Y:ICB2 interaction assessed using EMSAs...
October 24, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27730642/immunocytochemical-characterisation-of-neural-stem-progenitor-cells-from-green-terror-cichlid-aequidens-rivulatus
#15
C M Wen, M M Chen, F H Nan, C S Wang
In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein 32 (DARPP-32), oligodendrocyte transcription factor 2, paired box 6 and sex determining region Y-box 2. The intensity and localisation of these proteins, however, varied among the different NSPCs. Despite being intermediate cells, NSPCs can be divided into radial glial cells, oligodendrocyte progenitor cells (OPC) and neuroblasts by expressing the astrocyte marker glial fibrillary acidic protein (GFAP), OPC marker A2B5 and neuronal markers, including acetyl-tubulin, βIII-tubulin, microtubule-associated protein 2 and neurofilament protein...
October 11, 2016: Journal of Fish Biology
https://www.readbyqxmd.com/read/27713156/kindlin-2-interacts-with-%C3%AE-catenin-and-yb-1-to-enhance-egfr-transcription-during-glioma-progression
#16
Yunwei Ou, Zitong Zhao, Weimin Zhang, Qingnan Wu, Chuanyue Wu, Xuefeng Liu, Ming Fu, Nan Ji, Dan Wang, Jiaji Qiu, Liwei Zhang, Chunjiang Yu, Yongmei Song, Qimin Zhan
Kindlin-2 promotes carcinogenesis through regulation of cell-cell and cell-extracellular matrix adhesion. However, the role of Kindlin-2 in glioma has not been elucidated. We investigated Kindlin-2 expression in 188 human glioma tissue samples. High Kindlin-2 expression was correlated with high pathological grade and a worse prognosis. Kindlin-2 promoted glioma cell motility and proliferation both in vitro and in vivo. Importantly, Kindlin-2 activated the EGFR pathway and increased EGFR mRNA levels. In addition to up-regulating Y-box binding protein-1 (YB-1) and β-catenin expression, Kindlin-2 formed a transcriptional complex with YB-1 and β-catenin that bound to the EGFR promoter and enhanced transcription...
October 4, 2016: Oncotarget
https://www.readbyqxmd.com/read/27699813/foxo3a-expression-regulated-by-erk-signaling-is-inversely-correlated-with-y-box-binding-protein-1-expression-in-prostate-cancer
#17
Kenjiro Imada, Masaki Shiota, Kentaro Kuroiwa, Masaaki Sugimoto, Tatsuro Abe, Kenichi Kohashi, Akira Yokomizo, Masatoshi Eto, Seiji Naito, Yoshinao Oda
BACKGROUND: FOXO3a is a member of the forkhead O transcription factors. FOXO3a induces the factors that contribute to cell cycle arrest and is considered a tumor suppressor in several malignant tumors. Y-box binding protein-1 (YB-1) is a multifunctional protein whose high expression is correlated with poor prognoses in various malignant tumors. In the current study, we investigated the relationship between FOXO3a and YB-1 to validate their functional roles in prostate cancer. METHODS: Western blotting and cytotoxicity assays were conducted in prostate cancer cells, LNCaP, and 22Rv1 cells...
October 4, 2016: Prostate
https://www.readbyqxmd.com/read/27697431/nf-y-and-sp-transcription-factors-new-insights-in-a-long-standing-liaison
#18
Guntram Suske
For long it has been recognized that CCAAT boxes and GC-rich elements co-occur in many human and murine promoters within 100bp upstream of the transcription start site. The trimeric transcription factor NF-Y is the major CCAAT box-binding factor, and members of the SP family of transcription factors are the major GC box-binding proteins. Recent chromatin immunoprecipitations coupled with high throughput sequencing (ChIP-seq) have examined binding of NF-Y and the ubiquitous SP factors SP1, SP2 and SP3 genome-wide, allowing for comprehensive comparison of NF-Y and SP factor actions in the context of chromatin...
September 30, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27677949/structural-determinants-for-nf-y-dna-interaction-at-the-ccaat-box
#19
Valentina Nardone, Antonio Chaves-Sanjuan, Marco Nardini
The recently determined crystal structures of the sequence-specific transcription factor NF-Y have illuminated the structural mechanism underlying transcription at the CCAAT box. NF-Y is a trimeric protein complex composed by the NF-YA, NF-YB, and NF-YC subunits. NF-YB and NF-YC contain a histone-like domain and assemble on a head-to-tail fashion to form a dimer, which provides the structural scaffold for the DNA sugar-phosphate backbone binding (mimicking the nucleosome H2A/H2B-DNA assembly) and for the interaction with NF-YA...
September 25, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27667193/satellite-rnas-promote-pancreatic-oncogenic-processes-via-the-dysfunction-of-ybx1
#20
Takahiro Kishikawa, Motoyuki Otsuka, Takeshi Yoshikawa, Motoko Ohno, Hideaki Ijichi, Kazuhiko Koike
Highly repetitive tandem arrays at the centromeric and pericentromeric regions in chromosomes, previously considered silent, are actively transcribed, particularly in cancer. This aberrant expression occurs even in K-ras-mutated pancreatic intraepithelial neoplasia (PanIN) tissues, which are precancerous lesions. To examine the biological roles of the satellite RNAs in carcinogenesis, we construct mouse PanIN-derived cells expressing major satellite (MajSAT) RNA and show increased malignant properties. We find an increase in frequency of chromosomal instability and point mutations in both genomic and mitochondrial DNA...
2016: Nature Communications
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