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y box binding protein

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https://www.readbyqxmd.com/read/29320940/methylation-mediated-loss-of-sfrp2-enhances-invasiveness-of-non-small-cell-lung-cancer-cells
#1
X Zhang, X Rong, Y Chen, L Su
The malignancy of non-small cell lung cancer (NSCLC) largely results from its invasive manner. Secreted frizzled-related proteins (SFRPs) have been recently found to suppress the invasiveness of some cancers. On the other hand, the methylation of SFRPs increases protein degradation to reduce the activity of SFRPs, resulting in increased tumor cell invasion and cancer metastasis. However, the role of SFRPs in the invasion of NSCLC has not been reported. Here we analyzed the regulation of SFRPs in NSCLC cells and its effects on cell invasion...
February 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29296224/dormant-glioblastoma-cells-acquire-stem-cell-characteristics-and-are-differentially-affected-by-temozolomide-and-at101-treatment
#2
Vivian Adamski, Annika Hempelmann, Charlotte Flüh, Ralph Lucius, Michael Synowitz, Kirsten Hattermann, Janka Held-Feindt
Cellular dormancy is defined as a state in which cells enter quiescence driven by intrinsic or extrinsic factors, and striking parallels exist between the concept of cellular dormancy in malignancies and the cancer stem cell theory. We showed now that the proven dormancy markers insulin-like growth factor-binding protein 5, ephrin receptor A5 and histone cluster 1 H2B family member K were expressed in human glioblastomas in situ, were located in single tumor cells, and could be co-stained with each other and with the stem cell markers krüppel-like factor 4, octamer binding transcription factor 4 and sex determining region Y-box 2...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29277610/the-transcriptional-factor-yb-1-positively-regulates-hsc70-transcription-in-crassostrea-hongkongensis
#3
Huiru Liu, Delin Xu, Miao Cui, Tinglong Hou, Qizhong Zhang
A Y-box binding protein ChYB-1 was discovered as a ChHsc70 promoter-associated protein in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. The overexpression of ChYB-1 in heterologous HEK293T cells led to clear enhancement of ChHsc70 promoter expression, while ChYB-1 depletion correlated with significant reduction of ChHsc70 transcription in the hemocytes of C. hongkongensis. Quantitative Real-time PCR analysis revealed that both ChHsc70 and ChYB-1 were transcriptionally responsive to external chemical or physical stressors...
December 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29246296/antisense-oligonucleotides-targeting-y-box-binding-protein-1-inhibit-tumor-angiogenesis-by-downregulating-bcl-xl-vegfr2-tie-axes
#4
Kiyoko Setoguchi, Lin Cui, Nobutaka Hachisuka, Sumalee Obchoei, Kentaro Shinkai, Fuminori Hyodo, Kiyoko Kato, Fumito Wada, Tsuyoshi Yamamoto, Mariko Harada-Shiba, Satoshi Obika, Kenji Nakano
Y-box binding protein-1 (YB-1), involved in cancer progression and chemoradiation resistance, is overexpressed in not only cancer cells but also tumor blood vessels. In this study, we investigated the potential value of amido-bridged nucleic acid (AmNA)-modified antisense oligonucleotides (ASOs) targeting YB-1 (YB-1 ASOA) as an antiangiogenic cancer therapy. YB-1 ASOA was superior to natural DNA-based ASO or locked nucleic acid (LNA)-modified YB-1 ASO in both knockdown efficiency and safety, the latter assessed by liver function...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29237490/linc-ror-promotes-esophageal-squamous-cell-carcinoma-progression-through-the-derepression-of-sox9
#5
Lianghai Wang, Xiaodan Yu, Zhiyu Zhang, Lijuan Pang, Jiang Xu, Jinfang Jiang, Weihua Liang, Yuhang Chai, Jun Hou, Feng Li
BACKGROUND: Novel therapies tailored to the molecular composition of esophageal squamous cell carcinoma (ESCC) are needed to improve patient survival. We investigated the regulatory network of long intergenic non-protein coding RNA, regulator of reprogramming (linc-ROR) and sex-determining region Y-box 9 (SOX9), and their therapeutic relevance in ESCC. METHODS: Linc-ROR and SOX9 expression were examined in ESCC specimens, cell lines, and cultured tumorspheres. We investigated the effects of linc-ROR on SOX9 expression and malignant phenotypes by CCK8, colony formation, Transwell, and sphere-forming assay...
December 13, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29233047/rnai-of-grp78-may-disturb-the-fusion-of-icr-mouse-palate-cultured-in-vitro
#6
C Ren, X Li, X Li, Y Xie, H Fu, Z Yan, Y Zhu
RNA interference (RNAi) is a powerful tool to silence or minimize gene expression, and palate culture in vitro is an important technique for study of the palate development. Our previous study demonstrated that the gene expression of glucose-regulated protein-78 (Grp78) was downregulation in the all-trans retinoic acid-induced mouse models of cleft palate (CP) during embryogenesis. To find the role of Grp78, the small interfering RNA (siRNA) of this gene carried by fluorescent vector was injected with a microinjector, through which about 30 pmol siRNA was injected into the Institute of Cancer Research (ICR) mouse palate explants...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29228019/loci-and-pathways-associated-with-uterine-capacity-for-pregnancy-and-fertility-in-beef-cattle
#7
Mahesh Neupane, Thomas W Geary, Jennifer N Kiser, Gregory W Burns, Peter J Hansen, Thomas E Spencer, Holly L Neibergs
Infertility and subfertility negatively impact the economics and reproductive performance of cattle. Of note, significant pregnancy loss occurs in cattle during the first month of pregnancy, yet little is known about the genetic loci influencing pregnancy success and loss in cattle. To identify quantitative trait loci (QTL) with large effects associated with early pregnancy loss, Angus crossbred heifers were classified based on day 28 pregnancy outcomes to serial embryo transfer. A genome wide association analysis (GWAA) was conducted comparing 30 high fertility heifers with 100% success in establishing pregnancy to 55 subfertile heifers with 25% or less success...
2017: PloS One
https://www.readbyqxmd.com/read/29227750/genetic-polymorphisms-in-prm1-prm2-and-ybx2-genes-are-associated-with-male-factor-infertility
#8
Oya Sena E Aydos, Yalda Hekmatshoar, Buket Altınok, Tülin Özkan, Onur Şakirağaoğlu, Aynur Karadağ, Fuat Kaplan, Seda Ilgaz, Mehmet Taşpınar, Işıl Yükselen, Asuman Sunguroğlu, Kaan Aydos
AIMS: The etiology of infertility is still unknown in almost half of all male infertility patients. In sperm, DNA condensation differs from somatic and female gamete cells, with the protamine (PRM) gene and its transcription factor, Y-box binding protein 2 (YBX2), playing key roles in making the structure more compact. Protamine polymorphisms have been studied in different populations, but various results have been acquired. MATERIALS AND METHODS: In our study, we examined, for the first time in a Turkish population, the association between protamine gene alleles (PRM1 c...
December 11, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/29222418/heb-is-required-for-the-specification-of-fetal-il-17-producing-%C3%AE-%C3%AE-t-cells
#9
Tracy S H In, Ashton Trotman-Grant, Shawn Fahl, Edward L Y Chen, Payam Zarin, Amanda J Moore, David L Wiest, Juan Carlos Zúñiga-Pflücker, Michele K Anderson
IL-17-producing γδ T (γδT17) cells are critical components of the innate immune system. However, the gene networks that control their development are unclear. Here we show that HEB (HeLa E-box binding protein, encoded by Tcf12) is required for the generation of a newly defined subset of fetal-derived CD73- γδT17 cells. HEB is required in immature CD24+CD73- γδ T cells for the expression of Sox4, Sox13, and Rorc, and these genes are repressed by acute expression of the HEB antagonist Id3. HEB-deficiency also affects mature CD73+ γδ T cells, which are defective in RORγt expression and IL-17 production...
December 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/29218639/histidine-tracts-in-human-transcription-factors-insight-into-metal-ion-coordination-ability
#10
Aleksandra Hecel, Joanna Wątły, Magdalena Rowińska-Żyrek, Jolanta Świątek-Kozłowska, Henryk Kozłowski
Consecutive histidine repeats are chosen both by nature and by molecular biologists due to their high affinity towards metal ions. Screening of the human genome showed that transcription factors are extremely rich in His tracts. In this work, we examine two of such His-rich regions from forkhead box and MAFA proteins-MB3 (contains 18 His) and MB6 (with 21 His residues), focusing on the affinity and binding modes of Cu2+ and Zn2+ towards the two His-rich regions. In the case of Zn2+ species, the availability of imidazole nitrogen donors enhances metal complex stability...
December 7, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29201171/an-integrated-bioinformatical-analysis-of-mir-19a-target-genes-in-multiple-myeloma
#11
Hongyan Lv, Xianda Wu, Guiru Ma, Lixia Sun, Jianbo Meng, Xiaoning Song, Jinqiao Zhang
MicroRNA (miR)-19a, as an oncomiR, has been studied in several types of cancer; however, its role in the development and progression of multiple myeloma (MM) remains unclear. The present study used a bioinformatics approach to investigate the involvement of miR-19a in MM. miR-19a targets were predicted using target prediction programs, followed by screening for differentially expressed genes in MM. The function of these genes was then annotated using gene ontology term enrichment, signaling pathway enrichment and protein-protein interaction (PPI) analysis...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29197185/structure-and-interaction-of-corynebacterium-pseudotuberculosis-cold-shock-protein-a-with-y-box-single-stranded-dna-fragment
#12
Ícaro P Caruso, Vineet Panwalkar, Monika A Coronado, Andrew J Dingley, Marinônio L Cornélio, Dieter Willbold, Raghuvir K Arni, Raphael J Eberle
Cold shock proteins (Csps) function to preserve cell viability at low temperatures by binding to nucleic acids and consequently control gene expression. The mesophilic bacterium Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis in animals, and infection to livestock is a considerable economic burden worldwide. In this report, the structure of cold shock protein A from C. pseudotuberculosis (Cp-CspA) and biochemical analysis of its temperature-dependent interaction with a Y-box single-stranded DNA (ssDNA) motif is presented...
December 2, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29187452/y-box-binding-protein-1-enhances-oncogenic-transforming-growth-factor-%C3%AE-signaling-in-breast-cancer-cells-via-triggering-phospho-activation-of-smad2
#13
Matthias B Stope, Martin Weiss, Dominique Koensgen, Simone L Popp, Christian Joffroy, Alexander Mustea, Miriam B Buck, Cornelius Knabbe
BACKGROUND/AIM: Transforming growth factor β (TGFβ) plays a role in diverse oncogenic pathways including cell proliferation and cell motility and is regulated by the pleiotropic factor Y-box binding protein-1 (YB-1). In breast cancer, Sma/Mad related protein 2 (Smad2) represents the most common downstream transducer in TGFβ signaling. MATERIALS AND METHODS: Here, YB-1's impact on Smad2 phospho-activation was characterized by incubation of the breast cancer cell line MCF-7 with or without TGFβ1 in the absence or presence of overexpressed YB-1 protein...
December 2017: Anticancer Research
https://www.readbyqxmd.com/read/29180509/subcellular-localisation-of-the-stem-cell-markers-oct4-sox2-nanog-klf4-and-c-myc-in-cancer-a-review
#14
Bede van Schaijik, Paul F Davis, Agadha C Wickremesekera, Swee T Tan, Tinte Itinteang
The stem cell markers octamer-binding transcription factor 4, sex-determining region Y-box 2, NANOG, Kruppel-like factor 4 and c-MYC are key factors in inducing pluripotency in somatic cells, and they have been used to detect cancer stem cell subpopulations in a range of cancer types. Recent literature has described the subcellular localisation of these markers and their potential implications on cellular function. This is a relatively complex and unexplored area of research, and the extent of the effect that subcellular localisation has on cancer development and growth is largely unknown...
November 27, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29141367/-differentiation-of-induced-pluripotent-stem-cells-into-neural-stem-cells-induced-by-brain-derived-neurotrophic-factor-via-wnt-%C3%AE-catenin-and-extracellular-signal-regulated-kinase-mitogen-activated-protein-kinases-signal-pathway
#15
S Q Chen, M Huang, C L Liu, Y Y Shen, Q Cai, P J Wang
Objective: To investigate the mechanism of brain-derived neurotrophic factor (BDNF) promoting induced pluripotent stem cells (iPSCs) to differentiate into neural stem cells (NSCs) via Wnt/β-catenin and extracellular signal-regulated kinase/mitogen-activated protein kinases (ERK/MAPK) signal pathways. Methods: iPSCs were cultured and identified. The iPSCs were induced to differentiate into NSCs by BDNF and retinoic acid (RA). Nestin was detected by immunofluorescence and flow cytometry after iPSCs differentiated...
November 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29138807/role-of-lasp-1-a-novel-sox9-transcriptional-target-in-the-progression-of-lung-cancer
#16
Jianguang Shi, Jing Guo, Xinjian Li
Lung cancer accounts for most cancer-related deaths worldwide. However, the underlying mechanism by which it mediates the progression of lung cancer remains unclear. Expression of LASP-1 (LIM and SH3 protein 1) was evaluated in lung cancer tissues and tumor-adjacent normal tissues using immunohistochemistry and western blotting. Functional studies have shown that siRNA-mediated silencing of LASP-1 in human lung cancer cells and reduced cell proliferation, migration, and invasion. Flow cytometry and immunofluorescence staining also revealed that rate of cell apoptosis was increased after knockdown of expression of LASP-1, thereby suggesting that LASP-1 may function as an oncogene during lung cancer progression...
November 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29137403/chicken-ccdc152-shares-an-nfyb-regulated-bidirectional-promoter-with-a-growth-hormone-receptor-antisense-transcript-and-inhibits-cells-proliferation-and-migration
#17
Shudai Lin, Wei Luo, Mingya Jiang, Wen Luo, Bahareldin Ali Abdalla, Qinghua Nie, Li Zhang, Xiquan Zhang
The chicken coiled-coil domain-containing protein 152 (CCDC152) recently has been identified as a novel one implicated in cell cycle regulation, cellular proliferation and migration by us. Here we demonstrate that CCDC152 is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor (GHR) gene. Through serial luciferase reporter assays, we firstly identified a minimal 102 bp intergenic region as a core bidirectional promoter to drive basal transcription in divergent orientations...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29122685/molecular-and-enzoinformatics-perspectives-of-targeting-polo-like-kinase-1-in-cancer-therapy
#18
REVIEW
Shazi Shakil, Mohammad H Baig, Shams Tabrez, Syed M Danish Rizvi, Syed K Zaidi, Ghulam M Ashraf, Shakeel A Ansari, Aftab Aslam Parwaz Khan, Mohammad H Al-Qahtani, Adel M Abuzenadah, Adeel G Chaudhary
Cancer is a disease that has been the focus of scientific research and discovery and continues to remain so. Polo-like kinases (PLKs) are basically serine/threonine kinase enzymes that control cell cycle from yeast to humans. PLK-1 stands for 'Polo-like kinase-1'. It is the most investigated protein among PLKs. It is crucial for intracellular processes, hence a 'hot' anticancer drug-target. Accelerating innovations in Enzoinformatics and associated molecular visualization tools have made it possible to literally perform a 'molecular level walk' traversing through and observing the minutest contours of the active site of relevant enzymes...
November 6, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/29113949/dysregulated-expression-of-the-microrna-mir-137-and-its-target-yb-1-contribute-to-the-invasive-characteristics-of-malignant-pleural-mesothelioma
#19
Thomas G Johnson, Karin Schelch, Yuen Y Cheng, Marissa Williams, Kadir H Sarun, Michaela B Kirschner, Steven Kao, Anthony Linton, Sonja Klebe, Brian C McCaughan, Ruby Cy Lin, Christine Pirker, Walter Berger, Annette Lasham, Nico van Zandwijk, Glen Reid
BACKGROUND: Malignant pleural mesothelioma (MPM) is an aggressive malignancy linked to asbestos exposure. On a genomic level, MPM is characterised by frequent chromosomal deletions of tumour suppressors, including microRNAs. MiR-137 plays a tumour suppressor role in other cancers, so the aim of this study was to characterise it and its target Y-box binding protein-1 (YB-1) in MPM. BASIC PROCEDURES: Expression, methylation and copy number status of miR-137 and its host gene MIR137HG were assessed by PCR...
November 4, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/29089645/identification-and-characterization-of-hla-a24-specific-xbp1-cd138-syndecan-1-and-cs1-slamf7-peptides-inducing-antigens-specific-memory-cytotoxic-t-lymphocytes-targeting-multiple-myeloma
#20
J Bae, T Hideshima, G L Zhang, J Zhou, D B Keskin, N C Munshi, K C Anderson
XBP1 (X-box binding protein 1), CD138 (Syndecan-1), and CS1 (SLAMF7) are highly expressed antigens in cancers including multiple myeloma (MM). Here we identify and characterize immunogenic HLA-A24 peptides derived from these antigens for potential vaccination therapy of HLA-A24+ patients with MM. The identified immunogenic HLA-A24-specific XBP1 unspliced (UN)185-193 (I S P W I L A V L), XBP1 spliced (SP)223-231 (V Y P E G P S S L), CD138265-273 (I F A V C L V G F) and CS1240-248 (L F V L G L F L W) peptides induced antigen-specific CTL with anti-MM activity in an HLA-A24 restricted manner...
November 1, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
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