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https://www.readbyqxmd.com/read/28734212/treatment-of-refractory-contaminants-by-sludge-derived-biochar-persulfate-system-via-both-adsorption-and-advanced-oxidation-process
#1
Jia Wang, Zhuwei Liao, Jerosha Ifthikar, Lerong Shi, Yunan Du, Jingyi Zhu, Shuang Xi, Zhuqi Chen, Zhulei Chen
A novel strategy for the removal of refractory organic contaminants was realized through sludge-derived biochar (SDBC)/persulfate (PS) system via both adsorption and advanced oxidation process under ambient conditions. SDBC was prepared by one single step of slow pyrolysis of municipal sewage sludge, appeared a porous structure, and contained abundant oxygen-containing functional groups as well as amorphous Fe species. Large surface area and porous structure of SDBC benefitted the adsorption and enrichment of contaminants, while oxygen-containing functional groups and Fe species on the surface were considered as reactive components for the activation of PS...
July 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28731613/polyol-structure-influences-enzymatic-hydrolysis-of-bio-based-2-5-furandicarboxylic-acid-fdca-polyesters
#2
Karolina Haernvall, Sabine Zitzenbacher, Hassan Amer, Michael Thomas Zumstein, Michael Sander, Kristopher McNeill, Motonori Yamamoto, Michael Bernhard Schick, Doris Ribitsch, Georg M Guebitz
Polyesters of 2,5-furan dicarboxylic acid (FDCA) have gained attention since they can be regarded as the bio-based alternatives to the petroleum based polyesters of terephthalic acid. However, only little is known about the biodegradation and enzymatic hydrolysis of FDCA-based polyesters. This work aimed to investigate the influence of different polyols on enzymatic hydrolysis of FDCA-based polyesters. A series of polyesters containing various polyols were synthetized and analysed regarding susceptibility to enzymatic hydrolysis by cutinase 1 from Thermobifida cellulosilytica (Thc_Cut1)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28729132/meclas-an-online-tool-for-hazard-identification-and-classification-of-complex-inorganic-metal-containing-materials
#3
Frederik Verdonck, Hugo Waeterschoot, Patrick Van Sprang, Isabelle Vercaigne, Katrien Delbeke, Chretien Simons, Violaine Verougstraete
MeClas is a web-based tool to generate (eco)toxicity hazard categories and corresponding classification & labelling information of inorganic metal-containing complex materials such as ores, concentrates, intermediates or alloys for which the manual application of the GHS/CLP rules is very complex and requires a high level of consistency. The tool comprises several tiers, aimed at the progressive refinement of classification through recognition of specific mineral content, speciation/mineralogy up to bio-availability corrections...
July 17, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28727448/extensive-survey-of-antibody-invariant-positions-for-efficient-chemical-conjugation-using-expanded-genetic-codes
#4
Akifumi Kato, Mitsuo Kuratani, Tatsuo Yanagisawa, Kazumasa Ohtake, Akiko Hayashi, Yoshimi Amano, Kaname Kimura, Shigeyuki Yokoyama, Kensaku Sakamoto, Yasuhisa Shiraishi
The site-specific chemical conjugation of proteins, following synthesis with an expanded genetic code, promises to advance antibody-based technologies, including antibody drug conjugation and the creation of bispecific Fab dimers. The incorporation of non-natural amino acids into antibodies not only guarantees site specificity but also allows the use of bio-orthogonal chemistry. However, the efficiency of amino acid incorporation fluctuates significantly among different sites, thereby hampering the identification of useful conjugation sites...
July 20, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28723153/understanding-the-reactivity-of-co3-%C3%A2-and-no2-%C3%A2-radicals-towards-s-containing-and-aromatic-amino-acids
#5
Sharmistha Karmakar, Ayan Datta
The reactivity of CO3-(.) and NO2(.) radicals towards six amino acid side chains namely, cysteine (Cys), methionine (Met), phenylalanine (Phe), tyrosine (Tyr), histidine (His) and tryptophan (Trp), has been explored using state-of-art density functional theory (DFT) and transition state theory (TST). Three reaction mechanisms, namely hydrogen atom abstraction (HAT), radical adduct formation (RAF) and single electron transfer (SET) have been considered for detailed study. While CO3-(.) radical is highly reactive towards majority of amino acids, the reactivity of NO2(...
July 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28722147/surface-isoelectric-focusing-sief-with-carrier-ampholyte-ph-gradient
#6
Zhichao Wang, Cornelius Ivory, Adrienne R Minerick
Isoelectric focusing (IEF) is a powerful tool for amphoteric protein separations because of high sensitivity, bio-compatibility, and reduced complexity compared to chromatography or mechanical separation techniques. IEF miniaturization is attractive because it enables rapid analysis, easier adaptation to point of care applications, and smaller sample demands. However, existing small-scale IEF tools have not yet been able to analyze single protein spots from array libraries, which are ubiquitous in many pharmaceutical discovery and screening protocols...
July 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28719195/fluorinated-boronic-acid-appended-pyridinium-salts-and-19f-nmr-spectroscopy-for-diol-sensing
#7
Jörg Axthelm, Sven H C Askes, Martin Elstner, Upendar Reddy Gandra, Helmar Görls, Peter Bellstedt, Alexander Schiller
The identification and discrimination of diols is of fundamental importance in medical diagnostics, such as measuring the contents of glucose in the urine of diabetes patients. Diol sensors are often based on fluorophore-appended boronic acids, but these severely lack discrimination power and their response is one-dimensional. As an alternative strategy, we present the use of fluorinated boronic acid-appended pyridinium salts in combination with 19F NMR spectroscopy. A pool of 59 (bio)analytes was screened, containing monosaccharides, phosphorylated and N-acetylated sugars, polyols, carboxylic acids, nucleotides and amines...
July 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28717148/human-ribosomal-p1-p2-heterodimer-represents-an-optimal-docking-site-for-ricin-a-chain-with-a-prominent-role-for-p1-c-terminus
#8
Przemysław Grela, Xiao-Ping Li, Patrycja Horbowicz, Monika Dźwierzyńska, Marek Tchórzewski, Nilgun E Tumer
The eukaryotic P-stalk contains two P1-P2 protein dimers with a conserved C- terminal domain (CTD) critical for the interaction with external factors. To understand the role of the individual CTD of human P1/P2 proteins, we examined the interaction of reconstituted human P-protein complexes and C-terminally truncated forms with ricin A chain (RTA), which binds to the stalk to depurinate the sarcin/ricin loop (SRL). The interaction between P-protein complexes and RTA was examined by surface plasmon resonance, isothermal titration calorimetry, microscale thermophoresis and bio-layer interferometry...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716923/molecular-mechanism-of-environmental-d-xylose-perception-by-a-xylfii-lyts-complex-in-bacteria
#9
Jianxu Li, Chengyuan Wang, Gaohua Yang, Zhe Sun, Hui Guo, Kai Shao, Yang Gu, Weihong Jiang, Peng Zhang
d-xylose, the main building block of plant biomass, is a pentose sugar that can be used by bacteria as a carbon source for bio-based fuel and chemical production through fermentation. In bacteria, the first step for d-xylose metabolism is signal perception at the membrane. We previously identified a three-component system in Firmicutes bacteria comprising a membrane-associated sensor protein (XylFII), a transmembrane histidine kinase (LytS) for periplasmic d-xylose sensing, and a cytoplasmic response regulator (YesN) that activates the transcription of the target ABC transporter xylFGH genes to promote the uptake of d-xylose...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28716374/high-throughput-quantitation-of-fc-containing-recombinant-proteins-in-cell-culture-supernatant-by-fluorescence-polarization-spectroscopy
#10
Ben Thompson, Jerry Clifford, Mike Jenns, Andrew Smith, Ray Field, Kalpana Nayyar, David C James
Measurement of recombinant protein product titer critically underpins all biopharmaceutical manufacturing process development, as well as diverse research and discovery activity. Here, we describe a simple rapid (<2 min per 96 samples) 96-well microplate-based assay that enables high-throughput quantitation of recombinant immunoglobulin G and Fc-containing IgG derivatives in mammalian cell culture supernatant over a wide dynamic range of 2.5-80 mg/L, using microplate fluorescence polarization (FP) spectroscopy...
July 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28712082/biological-nutrient-recovery-from-culturing-of-pearl-gourami-trichogaster-leerii-by-cherry-tomato-solanum-lycopersicum-in-aquaponic-system
#11
Shima Makhdom, Seyed Pezhman Hosseini Shekarabi, Mehdi Shamsaie Mehrgan
The possibility of using different densities of cherry tomato as a bio-filter in a simple media-based aquaponic system to recycle nutrients from pearl gourami intensive culture wastewater was evaluated. Water quality parameters including total ammonia nitrogen (TAN), nitrite (NO2(-)), nitrate (NO3(-)), phosphate (PO4(3-)), pH, and dissolved oxygen (DO) were determined in outlet of the aquaponic system during a 60-day experimental period. Cherry tomato was planted at four densities of 0 (control), 3 (T1), 6 (T2), and 9 (T3) plants per aquaponic unit with a constant fish stock density...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28711017/alginate-gelatin-scaffolds-incorporated-with-silibinin-loaded-chitosan-nanoparticles-for-bone-formation-in-vitro
#12
R S Leena, M Vairamani, N Selvamurugan
Silibinin is a plant derived flavonolignan known for its multiple biological properties, but its role in the promotion of bone formation has not yet been well studied. Moreover, the delivery of Silibinin is hindered by its complex hydrophobic nature, which limits its bioavailability. Hence, in this study, we fabricated a drug delivery system using chitosan nanoparticles loaded with Silibinin at different concentrations (20μM, 50μM, and 100μM). They were then incorporated into scaffolds containing Alginate and Gelatin (Alg/Gel) for the sustained and prolonged release of Silibinin...
July 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28709077/phytonanotherapy-for-management-of-diabetes-using-green-synthesis-nanoparticles
#13
K Anand, C Tiloke, Pragalathan Naidoo, A A Chuturgoon
The world has a rich diversity of indigenous medicinal plants. The World Health Organization (WHO) gives high priority to eco-friendly, non-hazardous and cost effective healthcare such as the use of medicinal plants to treat various illnesses, including Human immunodeficiency virus (HIV) infection and Acquired immune deficiency syndrome (AIDS), tuberculosis (TB), diabetes mellitus (DM), malaria, and cancer. In developing countries, a high proportion of the population tends to use complementary and alternative medicines (CAM) together with conventional prescription drugs...
June 27, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28707322/development-and-validation-of-a-risk-model-for-long-term-mortality-after-percutaneous-coronary-intervention-the-idea-bio-study
#14
Nick van Boven, Ron T van Domburg, Isabella Kardys, Victor A Umans, K Martijn Akkerhuis, Mattie J Lenzen, Marco Valgimigli, Joost Daemen, Felix Zijlstra, Eric Boersma, Robert-Jan van Geuns
OBJECTIVES: We aimed to develop a model to predict long-term mortality after percutaneous coronary intervention (PCI), to aid in selecting patients with sufficient life expectancy to benefit from bioabsorbable scaffolds. BACKGROUND: Clinical trials are currently designed to demonstrate superiority of bioabsorbable scaffolds over metal devices up to 5 years after implantation. METHODS: From 2000 to 2011, 19.532 consecutive patients underwent PCI in a tertiary referral hospital...
July 14, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28706879/multiple-substrate-usage-of-coxiella-burnetii-to-feed-a-bipartite-metabolic-network
#15
Ina Häuslein, Franck Cantet, Sarah Reschke, Fan Chen, Matteo Bonazzi, Wolfgang Eisenreich
The human pathogen Coxiella burnetii causes Q-fever and is classified as a category B bio-weapon. Exploiting the development of the axenic growth medium ACCM-2, we have now used (13)C-labeling experiments and isotopolog profiling to investigate the highly diverse metabolic network of C. burnetii. To this aim, C. burnetii RSA 439 NMII was cultured in ACCM-2 containing 5 mM of either [U-(13)C3]serine, [U-(13)C6]glucose, or [U-(13)C3]glycerol until the late-logarithmic phase. GC/MS-based isotopolog profiling of protein-derived amino acids, methanol-soluble polar metabolites, fatty acids, and cell wall components (e...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28705503/building-a-bio-based-hydrogel-via-electrostatic-and-host-guest-interactions-for-realizing-dual-controlled-release-mechanism
#16
Shuai Han, Ting Wang, Li Yang, Bin Li
Bio-based hydrogel containing cyclodextrins (CDs) is of a promising polymer material that could display many advantages including wide availability, sustainability, biocompatibility and biodegradability, especially the inherent encapsulation ability with hydrophobic substance. To obtain these, the electrostatic and host-guest interactions were introduced and a hydrogel with three-dimensional double network structures was built. For preparing a spherical biopolymer cage, hydroxyethyl cellulose (HEC) and modified chitosan (HACC) were cross-linked by a one-pot reaction...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28703953/the-effect-of-temperature-on-in-vitro-digestibility-fat-globular-size-and-free-fatty-acid-bio-availability-in-milk-fat
#17
Michał Smoczyński
BACKGROUND: The rate at which a particular fat is digested has an effect on its bioavailability and on the lipid profile of the blood. Milk fat is a very complex mixture of triacylglycerols, resulting in a very wide melting range (from –40 to +40°C). Because temperature has a pronounced effect on the physicochemical state of milk fat (i.e. crystallisation of different fat fractions), this study analysed the bioavailability of milk fat at different temperatures. METHODS: A simplified model simulating digestion in the intestine at various temperatures was used...
April 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28699735/%C3%AF-conjugate-fluorophore-tagged-and-enzyme-responsive-l-amino-acid-polymer-nano-carrier-and-their-colour-tunable-intracellular-fret-probe-in-cancer-cells
#18
Sonashree Saxena, Manickam Jayakannan
The present investigation accounts one of the first example of enzyme-responsive and π-conjugate-tagged L-amino acid amphiphilic polymer and their fluorescence resonance energy transfer (FRET) probes for colour-tunable intracellular bio-imaging in cancer cells. Melt polymerizable oligo-phenylenevinylene (OPV) -conjugated diol was tailor-made and subjected to thermo-selective melt transesterification reaction with multi-functional L-aspartic acid monomer to yield OPV-tagged amphiphilic luminescent polyesters...
July 12, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28698068/new-nanoparticles-obtained-by-co-assembly-of-amphiphilic-cyclodextrins-and-nonlamellar-single-chain-lipids-preparation-and-characterization
#19
Cảnh Hưng Nguyễn, Jean-Luc Putaux, Gianluca Santoni, Sana Tfaili, Sophie Fourmentin, Jean-Baptiste Coty, Luc Choisnard, Annabelle Gèze, Denis Wouessidjewe, Gillian Barratt, Sylviane Lesieur, François-Xavier Legrand
This work aimed at preparing new nanoscale assemblies based on an amphiphilic bio-esterified β-cyclodextrin (β-CD), substituted at the secondary face with n-decanoic fatty acid chains (β-CD-C10), and monoolein (MO) as new carriers for parenteral drug delivery. Stable binary (β-CD-C10/MO) and ternary (β-CD-C10/MO/stabilizer) nanoscale assemblies close to 100nm in size were successfully prepared in water by the solvent displacement method. The generated nanoparticles were fully characterized by dynamic light scattering, transmission electron microscopy, small-angle X-ray scattering, residual solvent analysis, complement activation and the contribution of each formulation parameter was determined by principal component analysis...
July 8, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28692875/the-role-of-polyaminoamide-epichlorohydrin-pae-on-antibody-longevity-in-bioactive-paper
#20
Ziwei Huang, Thomas Gengenbach, Junfei Tian, Wei Shen, Gil Garnier
Paper has been used to engineer many types of bio-diagnostics. A major issue to most paper-based bio-diagnostics is the biomolecule instability causing the short shelf-life of the diagnostics. Commercial papers contain various polymeric additives. Polyamidoamine-epichlorohydrin (PAE), a polyelectrolyte typically used as wet-strength agent, is commonly used in filter papers and paper towels, which are often used as substrate in bioactive paper. However, the effect of cellulose or polymeric additives on antibody bioactivity is unknown...
July 3, 2017: Colloids and Surfaces. B, Biointerfaces
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