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Blastocystis

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https://www.readbyqxmd.com/read/29780015/distribution-of-intestinal-parasites-detected-in-%C3%A5-anl%C3%A4-urfa-mehmet-akif-inan-education-and-research-hospital-between-october-2015-and-october-2016
#1
Koray Öncel
OBJECTIVE: A total of 7353 patients who referred to the Microbiology Laboratory of Mehmet Akif Inan Education and Research Hospital between October 2015 and October 2016 were evaluated for intestinal parasites. METHODS: All fecal samples were investigated using three methods: wet mount, formalin ethyl acetate concentration, and trichrome staining. The cellophane tape method could only be used in a limited number of patients owing to the absence of a pediatrics outpatient clinic in our hospital...
March 2018: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29768563/occurrence-of-blastocystis-spp-in-domestic-animals-in-tri%C3%A3-ngulo-mineiro-area-of-brazil
#2
Renata Gregório Franco Moura, Márcia Benedita de Oliveira-Silva, André Luiz Pedrosa, Gabriel Antônio Nogueira Nascentes, Marlene Cabrine-Santos
INTRODUCTION: Currently, there are few studies regarding Blastocystis epidemiology. This study aimed to evaluate the occurrence of Blastocystis in animals in Uberaba, Brazil. METHODS: Fecal samples were examined by parasitological methods and screened for Blastocystis by polymerase chain reaction. RESULTS: Blastocystis spp. were observed in pigs, sheep, cattle, and dogs. Blastocystis polymerase chain reaction was positive in 14/22 samples positive by parasitological methods...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29761411/detection-of-pathogenic-viruses-in-the-ambient-air-in-seoul-korea
#3
Tae-Hee Han, Sang-Hun Park, Ju-Young Chung, Hyo-Won Jeong, Jihun Jung, Jae-In Lee, Young-Ok Hwang, Il-Young Kim, Jip-Ho Lee, Kweon Jung
The possible transport of pathogenic microorganisms during Asian dust events could be an important concern for health workers; however, this is still uncertain owing to a lack of supporting evidence. The present study aimed to investigate the presence of pathogenic microorganisms in air samples collected during the Asian and non-Asian dust periods. Between March and September 2016, air samples were collected at three weather observation stations in Seoul using a high-volume air sampler. Multiplex PCR was performed using the Allplex™ respiratory and gastrointestinal panel assay kits to detect 46 microorganisms...
May 14, 2018: Food and Environmental Virology
https://www.readbyqxmd.com/read/29747749/chemical-analysis-of-aqueous-extracts-of-origanum-majorana-and-foeniculum-vulgare-and-their-efficacy-on-blastocystis-spp-cysts
#4
Eman M H Méabed, Nagwa M El-Sayed, Alaa I B Abou-Sreea, Mohamed H H Roby
BACKGROUND: Origanum majorana (O. majorana) and Foeniculum vulgare (F. vulgare) are traditionally used herbs in Egypt for treatment of several diseases including parasitic diseases. The Purpose was to determine the efficacy of O. majorana and F. vulgare aqueous extracts (AEs) on Blastocystis spp. in vitro, and to reveal their phenolic, flavonoids components and antioxidant activities through chemical analysis. METHODS: The Efficacy of both plant AEs on human Peripheral Blood Mononuclear Cells (PBMCs) viability was assessed using MTT assay...
April 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29744356/genetic-diversity-and-distribution-of-blastocystis-subtype-3-in-human-populations-with-special-reference-to-a-rural-population-in-central-mexico
#5
Liliana Rojas-Velázquez, Patricia Morán, Angélica Serrano-Vázquez, Leonardo D Fernández, Horacio Pérez-Juárez, Augusto C Poot-Hernández, Tobías Portillo, Enrique González, Eric Hernández, Oswaldo Partida-Rodríguez, Miriam E Nieves-Ramírez, Ulises Magaña, Javier Torres, Luis E Eguiarte, Daniel Piñero, Cecilia Ximénez
Blastocystis subtype 3 (ST3) is a parasitic protist found in the digestive tract of symptomatic and asymptomatic humans around the world. While this parasite exhibits a high prevalence in the human population, its true geographic distribution and global genetic diversity are still unknown. This gap in knowledge limits the understanding of the spread mechanisms, epidemiology, and impact that this parasite has on human populations. Herein, we provided new data on the geographical distribution and genetic diversity of Blastocystis ST3 from a rural human population in Mexico...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29742864/atorvastatin-in-vivo-synergy-with-metronidazole-as-anti-blastocystis-therapy
#6
Maha M A Basyoni, Shawky A Fouad, Marwa F Amer, Ahmed Fathy Amer, Dalia Ibrahim Ismail
Blastocystis is an enteric Straminopile in tropical, subtropical and developing countries. Metronidazole has been a chemotheraputic for blastocystosis. Failures in its regimens were reported and necessitate new studies searching for alternative therapeutic agents. Aim of current study is to investigate potential effects of Atorvastatin (AVA) compared to the conventional chemotherapeutic MTZ in experimentally Blastocystis -infected mice. Anti- Blastocystis efficacy of AVA was evaluated parasitologically, histopathologically and by transmission electron microscopy using MTZ (10 mg/kg) as a control...
April 2018: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29737264/what-are-hidden-facts-behind-intestinal-parasitic-infections-in-ilam-city
#7
Jahangir Abdi, Soghra Viesy, Zahra Rezaei
Intestinal parasitic infections are the one of the most common health problems in developing countries. A number of patients die annually due to complications caused by these parasites.Therefore, the aim of this study was to investigate the rate and type of parasitic infections, determine the factors affecting them in Ilam city and also provide strategies to prevent them. In this descriptive cross-sectional study conducted in one of the Ilam labs in 2016, 417 stool specimens were randomly collected. All specimens were examined using direct and ethanol formaldehyde...
May 8, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29608961/genetic-diversity-analysis-of-blastocystis-subtypes-from-both-symptomatic-and-asymptomatic-subjects-using-a-barcoding-region-from-the-18s-rrna-gene
#8
Tahereh Rezaei Riabi, Hamed Mirjalali, Ali Haghighi, Mohammad Rostami Nejad, Mohammad Amin Pourhoseingholi, Philippe Poirier, Frederic Delbac, Ivan Wawrzyniak, Mohammad Reza Zali
Blastocystis is the most prevalent protozoa found in human stool samples. This study aimed to evaluate genetic diversity among Blastocystis subtypes isolated from both symptomatic and asymptomatic subjects as well as the potential correlation between subtypes and symptoms. A total of 55 Blastocystis-positive isolates were included in this study. A barcoding region of the small subunit rDNA was amplified and genetically assessed using MEGA6 and DnaSP regarding the presence of symptoms. BLAST analyses revealed the presence of 5 different subtypes (ST1, ST2, ST3, ST6 and ST7) among the samples...
March 30, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29602317/blastocystis-sp-an-emerging-parasite-with-controversial-pathogenicity-should-all-human-cases-be-treated
#9
EDITORIAL
M Subirats, R Borrás
No abstract text is available yet for this article.
April 2018: Revista Clínica Española
https://www.readbyqxmd.com/read/29587846/evaluation-of-two-dna-extraction-methods-for-the-pcr-based-detection-of-eukaryotic-enteric-pathogens-in-fecal-samples
#10
Estelle Menu, Charles Mary, Isabelle Toga, Didier Raoult, Stéphane Ranque, Fadi Bittar
OBJECTIVE: Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative PCR cycle threshold (Ct) values, the efficiency of two DNA extraction kits: the semi-automated EZ1® (Qiagen) and the manual QIAamp® DNA Stool Mini Kit (Qiagen), on six protozoa: Blastocystis spp., Cryptosporidium parvum/hominis, Cyclospora cayetanensis, Dientamoeba fragilis, Giardia intestinalis and Cystoisospora belli and one microsporidia: Enterocytozoon bieneusi...
March 27, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29581055/evaluation-of-the-roche-lightmix-%C3%A2-gastro-parasites-multiplex-pcr-assay-detecting-giardia-duodenalis-entamoeba-histolytica-cryptosporidia-dientamoeba-fragilis-and-blastocystis-hominis
#11
Johannes Friesen, Jörg Fuhrmann, Heike Kietzmann, Egbert Tannich, Michael Müller, Ralf Ignatius
OBJECTIVES: Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix® Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. METHODS: Stool specimens (n=1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen® enzyme immunoassays (EIAs) detecting G...
March 23, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29567298/associations-between-gut-microbiota-and-common-luminal-intestinal-parasites
#12
REVIEW
Christen Rune Stensvold, Mark van der Giezen
The development and integration of DNA-based methods in research and clinical microbiology laboratories have enabled standardised and comprehensive detection and differentiation of the microbes colonising our guts. For instance, the single-celled parasites Blastocystis and Dientamoeba appear to be much more common than previously thought, especially so in healthy individuals. While increasing evidence appears to suggest limited pathogenicity of these parasites, next-generation-sequencing-based studies have helped us to appreciate links between parasite colonisation and certain host phenotypical characteristics and gut microbial profiles...
May 2018: Trends in Parasitology
https://www.readbyqxmd.com/read/29536712/-epidemic-status-of-human-key-parasitic-diseases-in-tongcheng-city-anhui-province
#13
Jiang Long-Zhi
OBJECTIVE: To understand the current status of key parasitic diseases in Tongcheng City, so as to provide an epidemiological reference for formulating the scientific planning for "13th Five-year" parasitic diseases prevention and control. METHODS: The villages of different levels in Tongcheng City were sampled to investigate the infection of parasitic diseases of residents aged three or more years. Soil-transmitted nemathelminth, intestinal protozoa and Enterobius vermicularis were investigated by the Kato-Katz technique (a fecal sample seized two), iodine smear method and cellophane tape method, respectively...
October 24, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29529848/prevalence-of-intestinal-parasites-and-associated-risk-factors-for-infection-among-rural-communities-of-chachoengsao-province-thailand
#14
Pisit Suntaravitun, Amornrat Dokmaikaw
Intestinal parasitic infections remain a major public health problem in many parts of Thailand, particularly in rural areas. This study aimed to determine the prevalence of intestinal parasitic infections and associated risk factors among the people living in Huai Sai sub-district, Bang Khla district, Chachoengsao Province, central Thailand. A cross-sectional survey was carried out from June 2017 to August 2017 which included a total of 224 participants. Stool samples were examined using a simple direct smear and formalin ethyl acetate concentration technique...
February 2018: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29522552/intestinal-parasite-infections-in-a-rural-community-of-rio-de-janeiro-brazil-prevalence-and-genetic-diversity-of-blastocystis-subtypes
#15
Carolina Valença Barbosa, Magali Muniz Barreto, Rosemary de Jesus Andrade, Fernando Sodré, Claudia Masini d'Avila-Levy, José Mauro Peralta, Ricardo Pereira Igreja, Heloisa Werneck de Macedo, Helena Lucia Carneiro Santos
BACKGROUND: Intestinal parasitic infections are considered a serious public health problem and widely distributed worldwide, mainly in urban and rural environments of tropical and subtropical countries. Globally, soil-transmitted helminths and protozoa are the most common intestinal parasites. Blastocystis sp. is a highly prevalent suspected pathogenic protozoan, and considered an unusual protist due to its significant genetic diversity and host plasticity. METHODOLOGY/MAIN FINDINGS: A total of 294 stool samples were collected from inhabitants of three rural valleys in Rio de Janeiro, Brazil...
2018: PloS One
https://www.readbyqxmd.com/read/29498738/blastocystis-hominis-transmission-by-non-potable-water-a-case-report-in-italy
#16
Maria Cristina Angelici, Chiara Nardis, Riccardo Scarpelli, Paola Ade
In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method...
March 2, 2018: New Microbiologica
https://www.readbyqxmd.com/read/29491555/genomic-analysis-of-blastocystis-hominis-isolates-in-patients-with-hiv-positive-using-locus-ssu-rdna
#17
Anahita Razavi Piranshahi, Mehdi Tavalla, Shahram Khademvatan
Blastocystis hominis ( B. hominis ) is a protozoan zoonosis which clinical signs of infection with this parasite has been reported to be more severe in patients with weakened immune systems than healthy controls. So, the aim of the study was to evaluate genomic analysis of B. hominis isolates obtained from patients with HIV-positive using locus SSU-rDNA. At first, 268 stool samples were randomly collected from patients with HIV-positive referred to health centers of Khuzestan province, southwest of Iran. Formol-ether and direct smear techniques were used for the detection of parasitic agents...
March 2018: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29469358/-survey-and-analysis-of-epidemic-status-of-principal-human-parasitosis-in-ecological-region-of-huaiyang-hills-of-henan-province-in-2015
#18
Zhang Ya-Lan, Zhu Yan-Kun, Chen Wei-Qi, Deng Yan, Lin Xi-Meng, Li Peng, Zhang Hong-Wei
OBJECTIVE: To understand the epidemic status of principal human parasitosis in the ecological region of Huaiyang hills of Henan Province. METHODS: According to the scheme of The 3rd National Survey of Principal Human Parasites made by National Institute of Parasitic Diseases, the survey was performed based on the ecological regions. The stratified cluster sampling was made combined with the economic and geographical conditions. The infections of intestinal helminths and protozoans in permanent residents were respectively detected by Kato-Kats technique and iodine solution...
July 24, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29455921/clinical-and-epidemiological-characteristics-of-intestinal-parasite-infection-by-blastocystis-hominis
#19
C Ocaña-Losada, J A Cuenca-Gómez, M T Cabezas-Fernández, J Vázquez-Villegas, M J Soriano-Pérez, I Cabeza-Barrera, J Salas-Coronas
OBJECTIVES: Blastocystis hominis (B. hominis) is one of the most common intestinal parasites isolated in humans. The parasite can cause gastrointestinal symptoms or, in most cases, remain asymptomatic. There are issues concerning the parasite's pathogenic character. The aim of this study was to analyse the clinical and epidemiological characteristics of the parasite infection by B. hominis, with or without other parasitic co-infections. PATIENTS AND METHODS: An observational retrospective study was conducted of B...
April 2018: Revista Clínica Española
https://www.readbyqxmd.com/read/29454366/occurrence-of-blastocystis-sp-and-pentatrichomonas-hominis-in-sheep-and-goats-in-china
#20
Wen Chao Li, Kai Wang, Youfang Gu
BACKGROUND: Global data regarding the molecular epidemiology of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats are sparse. China has one of the largest sheep and goat populations in the world. In this study we investigated the occurrence of Blastocystis sp. and P. hominis in domestic sheep and goats in China, and analyzed the genetic characterization of these two parasite species. METHODS: In total, we collected fresh fecal samples from 832 sheep and 781 goats located on seven and ten farms, respectively, in the central eastern region of China...
February 17, 2018: Parasites & Vectors
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