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Blastocystis

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https://www.readbyqxmd.com/read/28238960/inter-and-intra-subtype-variation-of-blastocystis-subtypes-isolated-from-diarrheic-and-non-diarrheic-patients-in-iran
#1
Atefe Alinaghizade, Hamed Mirjalali, Mehdi Mohebali, Christen Rune Stensvold, Mostafa Rezaeian
Blastocystis is a common intestinal parasitic protist infecting birds and mammals. Blastocystis comprises at least 17 subtypes (ST), of which ST1-ST9 have been detected in humans. Significant correlation between certain subtypes and pathogenicity remains to be established. Nevertheless, some studies suggest a potential linkage between subtypes (inter- and intra-subtype variation) and clinical manifestations. The aim of this study was to identify intra-subtype genetic variation of subtypes of Blastocystis in stools samples submitted by diarrheic and non-diarrheic patients...
February 23, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28224033/distribution-and-risk-factors-associated-with-intestinal-parasite-infections-among-children-with-gastrointestinal-disorders
#2
Hamed Kiani, Ali Haghighi, Roya Salehi, Eznollah Azargashb
AIM: Prevalence and risk factors associated with intestinal parasites among children ≤ 12 years old in Nahavand county western Iran, was the objective of this search. BACKGROUND: Intestinal parasites (IPs) are important health problems among most societies. METHODS: This cross sectional study was carried out during 6 months from April to September 2014 in Nahavand County western Iran. Fecal samples were collected from 500 children suffering from gastrointestinal disorders (GIDs) and examined by macroscopy and microscopic (using saline and iodine wet mount, formalin-ether sedimentation, Trichrome and modified Ziehl Neelsen staining) methods...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28224032/prevalence-of-intestinal-parasites-in-referred-individuals-to-the-medical-centers-of-tonekabon-city-mazandaran-province
#3
Samira Shahdoust, Maryam Niyyati, Ali Haghighi, Eznoallah Azargashb, Mohammad Reza Khataminejad
AIM: The aim of the present study was to determine the prevalence of intestinal parasites and their relation with socio-demographic data in referred individuals to the medical centers in Tonekabon, Mazandaran province, 2015. BACKGROUND: Due to the climatic and ecological conditions in Mazandaran province, determination of the status of intestinal parasites among referred individuals to the medical centers of Tonekabon city can help researchers and healthcare services to prevent and/or control of parasitic infection in this region...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28222192/blastocystis-subtyping-and-its-association-with-intestinal-parasites-in-children-from-different-geographical-regions-of-colombia
#4
Juan David Ramírez, Carolina Flórez, Mario Olivera, María Consuelo Bernal, Julio Cesar Giraldo
Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia...
2017: PloS One
https://www.readbyqxmd.com/read/28167596/epidemiology-and-prevalence-of-blastocystis-spp-in-north-cyprus
#5
Ayse Seyer, Djursun Karasartova, Emrah Ruh, Ayse Semra Güreser, Ebru Turgal, Turgut Imir, Aysegul Taylan Ozkan
This study was conducted to investigate the prevalence of Blastocystis spp. and its subtypes (STs) in North Cyprus; and to evaluate the presence of this parasite and its STs with respect to demographic, socioeconomic, and epidemiological factors, as well as gastrointestinal symptoms. Stool samples were collected from 230 volunteers. Each participant also filled out a questionnaire. The samples were examined microscopically by native-Lugol and trichrome methods and further tested by polymerase chain reaction (PCR) and sequencing...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28127364/prevalence-of-strongyloides-stercoralis-and-other-intestinal-parasites-among-institutionalized-mentally-disabled-individuals-in-rasht-northern-iran
#6
Amin Saeidinia, Ilnaz Tavakoli, Mohammad Reza Naghipour, Behnaz Rahmati, Hossein Ghavami Lahiji, Omid Salkhori, Keyhan Ashrafi
BACKGROUND: We aimed to determine the status of strongyloidiasis in mentally disabled population in the institutional places in Rasht City, the capital of Guilan Province, northern Iran. METHODS: This cross-sectional study was conducted in 8 institutions for mentally retarded population in Rasht in 2013. Before collecting the samples, a questionnaire was filled out for each participant by an expert person. A single stool sample was obtained from each of the 173 subjects and examined using direct wet mount, formalin-ether concentration technique and agar plate culture method...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096858/prevalence-of-intestinal-parasitic-infections-in-jiroft-kerman-province-iran
#7
Maryam Barkhori Mahni, Mostafa Rezaeian, Eshrat Beigom Kia, Ahmad Raeisi, Khadijeh Khanaliha, Fatemeh Tarighi, Bahare Kamranrashani
BACKGROUND: Intestinal parasitic infections have a worldwide distribution. High prevalence of intestinal parasitic infections in individuals with low socioeconomic status and environmental conditions was found. No study has ever been conducted on the prevalence of these infections in Jiroft. Therefore, in this study prevalence of intestinal parasitic infections was evaluated in Jiroft, Kerman Province, Iran. METHODS: A total of 1060 individuals from rural and urban areas of Jiroft were sampled accidentally, during 2013-2014...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28088334/subtype-identification-of-human-blastocystis-spp-isolated-from-lao-people-s-democratic-republic
#8
Oranuch Sanpool, Sakhone Laymanivong, Tongjit Thanchomnang, Rutchanee Rodpai, Lakkhana Sadaow, Issarapong Phosuk, Wanchai Maleewong, Pewpan M Intapan
Blastocystis sp. is the most common protist found in the intestinal tract of humans and animals. Blastocystis subtypes (STs) are classified based on the molecular phylogeny of the small subunit rRNA gene (SSU rDNA). At least 17 Blastocystis STs have been reported and, of these, STs 1-9 have been found in humans. This study revealed the presence of human Blastocystis STs in Lao People's Democratic Republic (Lao PDR). Fecal samples were collected from apparently healthy people from the central province of Khammouane and the southern province of Champasak...
January 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28087441/blastocystis-and-irritable-bowel-syndrome-frequency-and-subtypes-from-iranian-patients
#9
Shahram Khademvatan, Rahim Masjedizadeh, Fakher Rahim, Hamidreza Mahbodfar, Roya Salehi, Ehsan Yousefi-Razin, Masoud Foroutan
There are inconsistent findings about the role of Blastocystis infection in irritable bowel syndrome (IBS). The present study was aimed to determine the frequency of Blastocystis and their subtypes (ST) in patients with IBS. A total of 122 patients with IBS and 122 healthy individuals referred to the medical laboratory centers in Ahvaz (southwest of Iran) participated in the study. The frequency of Blastocystis was determined. Blastocystis genomic DNA was extracted from positive feces, and PCR was performed using seven primer pairs targeting the SSU rDNA gene...
April 2017: Parasitology International
https://www.readbyqxmd.com/read/28060901/molecular-epidemiology-of-blastocystis-sp-in-various-animal-groups-from-two-french-zoos-and-evaluation-of-potential-zoonotic-risk
#10
Amandine Cian, Dima El Safadi, Marwan Osman, Romain Moriniere, Nausicaa Gantois, Sadia Benamrouz-Vanneste, Pilar Delgado-Viscogliosi, Karine Guyot, Luen-Luen Li, Sébastien Monchy, Christophe Noël, Philippe Poirier, Céline Nourrisson, Ivan Wawrzyniak, Frédéric Delbac, Stéphanie Bosc, Magali Chabé, Thierry Petit, Gabriela Certad, Eric Viscogliosi
Blastocystis sp. is a common intestinal parasite infecting humans and a wide range of animals worldwide. It exhibits an extensive genetic diversity and 17 subtypes (STs) have thus far been identified in mammalian and avian hosts. Since several STs are common to humans and animals, it was proposed that a proportion of human infections may result from zoonotic transmission. However, the contribution of each animal source to human infection remains to be clarified. Therefore, the aim of this study was to expand our knowledge of the epidemiology and host specificity of this parasite by performing the largest epidemiological survey ever conducted in animal groups in terms of numbers of species screened...
2017: PloS One
https://www.readbyqxmd.com/read/28056072/clarifying-the-cryptic-host-specificity-of-blastocystis-spp-isolates-from-alouatta-palliata-and-a-pigra-howler-monkeys
#11
Claudia Villanueva-Garcia, Elias Jose Gordillo-Chavez, Eduardo Lopez-Escamilla, Emilio Rendon-Franco, Claudia Irais Muñoz-Garcia, Lilia Gama, Williams Arony Martinez-Flores, Nayeli Gonzalez-Rodriguez, Mirza Romero-Valdovinos, Hilda Diaz-Lopez, Jose Galian, Guiehdani Villalobos, Pablo Maravilla, Fernando Martinez-Hernandez
Although the presence of cryptic host specificity has been documented in Blastocystis, differences in infection rates and high genetic polymorphism within and between populations of some subtypes (ST) have impeded the clarification of the generalist or specialist specificity of this parasite. We assessed the genetic variability and host specificity of Blastocystis spp. in wild howler monkeys from two rainforest areas in the southeastern region of Mexico. Fecal samples of 225 Alouatta palliata (59) and A. pigra (166) monkeys, belonging to 16 sylvatic sites, were analyzed for infection with Blastocystis ST using a region of the small subunit rDNA (SSUrDNA) gene as a marker...
2017: PloS One
https://www.readbyqxmd.com/read/28007484/diagnosis-of-intestinal-parasites-in-a-rural-community-of-venezuela-advantages-and-disadvantages-of-using-microscopy-or-rt-pcr
#12
COMPARATIVE STUDY
Renzo Nino Incani, Elizabeth Ferrer, Denise Hoek, Robbert Ramak, Jeroen Roelfsema, Lapo Mughini-Gras, Titia Kortbeek, Elena Pinelli
A cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time PCR (RT-PCR) for 14 intestinal parasites in a Venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. A total of 228 participants were included in this study. A multiplex RT-PCR was used for the detection of Dientamoeba fragilis, Giardia intestinalis, Cryptosporidium sp. and a monoplex RT-PCR for Entamoeba histolytica...
March 2017: Acta Tropica
https://www.readbyqxmd.com/read/27989831/subtype-distribution-of-blastocystis-spp-isolated-from-children-in-eskisehir-turkey
#13
Nihal Dogan, Merve Aydin, Nazmiye Ulku Tuzemen, Ener Cagri Dinleyici, Ilkiz Oguz, Funda Dogruman-Al
Blastocystis spp. is the most common enteric protist found in human feces. The pathogenic role of Blastocystis remains controversial and it has been suggested that the symptomatology of Blastocystis is associated with its subtypes (ST). However, only few studies have investigated the relationship between the symptomatology and subtypes of Blastocystis in children. This study aimed to investigate the prevalence of Blastocystis in children aged 3 to 13years with or without gastrointestinal complaints and determine the distribution of the subtypes of Blastocystis...
October 27, 2016: Parasitology International
https://www.readbyqxmd.com/read/27981305/association-of-enteric-protist-blastocystis-spp-and-gut-microbiota-with-hepatic-encephalopathy
#14
Sedat Yildiz, İbrahim Doğan, Funda Doğruman-Al, Ufuk Nalbantoğlu, Duran Üstek, Fakhriddin Sarzhanov, Süleyman Yildirim
BACKGROUND AND AIMS: Hepatic encephalopathy (HE) is a serious neuropsychiatric sequela emerging in the advanced stages of cirrhosis. The gut microbiota plays an important role in the development of HE. The aim of the study was to analyze the dynamic interplay between microbiota and Blastocystis in cirrhotic patients with or without encephalopathy. METHODS: The study was designed as cross-sectional study. A total of 37 patients from the Ankara city, admitted to the University Hospital within a 6-month period prior to enrolment into the study were included in the study...
December 2016: Journal of Gastrointestinal and Liver Diseases: JGLD
https://www.readbyqxmd.com/read/27934806/molecular-appraisal-of-intestinal-parasitic-infection-in-transplant-recipients
#15
Pooja Yadav, Shehla Khalil, Bijay Ranjan Mirdha
BACKGROUND & OBJECTIVES: Diarrhoea is the main clinical manifestation caused by intestinal parasitic infections in patients, with special reference to transplant recipients who require careful consideration to reduce morbidity and mortality. Further, molecular characterization of some important parasites is necessary to delineate the different modes of transmission to consider appropriate management strategies. We undertook this study to investigate the intestinal parasitic infections in transplant recipients with or without diarrhoea, and the genotypes of the isolated parasites were also determined...
August 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27920396/prevalence-and-subtype-identification-of-blastocystis-sp-in-healthy-individuals-in-the-tunis-area-tunisia
#16
Imène Ben Abda, Nabil Maatoug, Rania Ben Romdhane, Nada Bouhelmi, Najet Zallegua, Karim Aoun, Eric Viscogliosi, Aïda Bouratbine
Blastocystis sp. is currently the most common eukaryotic parasite found in humans. Despite its potential public health impact, epidemiological data regarding its prevalence and molecular subtype (ST) distribution in Maghreb are rarely reported. Therefore, the aim of this study was to determine the prevalence of the parasite in a cohort of healthy food handler Tunisian individuals and to acquire the first molecular data regarding the distribution of Blastocystis sp. STs in this country. Therefore, 524 fecal samples were collected, and 68 of them (13%) were identified as positive for the parasite by direct-light microscopy of smears...
January 11, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27907915/parasitic-infections-in-children-with-chronic-spontaneous-urticaria
#17
Ebru Arik Yilmaz, Betul Karaatmaca, Cansin Sackesen, Umit Murat Sahiner, Ozlem Cavkaytar, Bulent E Sekerel, Ozge Soyer
BACKGROUND: Parasites have been proposed to be an underlying cause of chronic spontaneous urticaria (CSU) in childhood, but a clear causal relationship between them has not been established. This study aimed to investigate the prevalence of parasitic infection-related CSU (PIRCSU) in children and to determine the factors associated with PIRCSU. METHOD: Data from 211 children with CSU were analyzed. Information on stool examination, antiparasitic medications received, and response to treatment was recorded...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27905282/distribution-of-parasites-detected-in-stool-samples-of-patients-admitted-to-our-parasitology-laboratory-during-a-three-year-period-between-2012-and-2014
#18
Mehmet Burak Selek, Bayhan Bektöre, Ergenekon Karagöz, Orhan Baylan, Mustafa Özyurt
OBJECTIVE: Parasitic diseases are among the major public health issues worldwide. A number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. Nevertheless, the most common diagnostic method is microscopic examination. In this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 2014...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27903290/assessing-the-burden-of-intestinal-parasites-affecting-newly-arrived-immigrants-in-qatar
#19
Marawan A Abu-Madi, Jerzy M Behnke, Ahmed Ismail, Sonia Boughattas
BACKGROUND: In the last decades, the enormous influx of immigrants to industrialized countries has led to outbreaks of parasitic diseases, with enteric infections being amongst the most frequently encountered. In its strategy to control such infection, Qatar has established the Pre-Employment Certificate (PEC) program which requires medical inspection before arrival in Qatar and which is mandatory for immigrant workers travelling to the country. To assess the reliability of the PEC, we conducted a survey of intestinal parasites, based on examination of stool samples provided by immigrant workers (n = 2,486) recently arrived in Qatar...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27892847/international-adoption-from-ethiopia-an-overview-of-the-health-status-at-arrival-in-belgium
#20
Lydia Van Kesteren, Marek Wojciechowski
BACKGROUND: Ethiopia is a densely populated country with a fast growing economy. Still socioeconomic and health issues render many children parentless. One thousand and twenty eight Ethiopian children have been adopted in Belgium from September 2005 to September 2015. Little has been published about their health status at arrival. METHODS: Three hundred and fifteen children adopted from Ethiopia were clinically evaluated at the Institute of Tropical Medicine in Antwerp from 1 January 2008 until 31 December 2014...
November 28, 2016: Acta Clinica Belgica
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