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https://www.readbyqxmd.com/read/27907915/parasitic-infections-in-children-with-chronic-spontaneous-urticaria
#1
Ebru Arik Yilmaz, Betul Karaatmaca, Cansin Sackesen, Umit Murat Sahiner, Ozlem Cavkaytar, Bulent E Sekerel, Ozge Soyer
BACKGROUND: Parasites have been proposed to be an underlying cause of chronic spontaneous urticaria (CSU) in childhood, but a clear causal relationship between them has not been established. This study aimed to investigate the prevalence of parasitic infection-related CSU (PIRCSU) in children and to determine the factors associated with PIRCSU. METHOD: Data from 211 children with CSU were analyzed. Information on stool examination, antiparasitic medications received, and response to treatment was recorded...
December 2, 2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27905282/distribution-of-parasites-detected-in-stool-samples-of-patients-admitted-to-our-parasitology-laboratory-during-a-three-year-period-between-2012-and-2014
#2
Mehmet Burak Selek, Bayhan Bektöre, Ergenekon Karagöz, Orhan Baylan, Mustafa Özyurt
OBJECTIVE: Parasitic diseases are among the major public health issues worldwide. A number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. Nevertheless, the most common diagnostic method is microscopic examination. In this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 2014...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27903290/assessing-the-burden-of-intestinal-parasites-affecting-newly-arrived-immigrants-in-qatar
#3
Marawan A Abu-Madi, Jerzy M Behnke, Ahmed Ismail, Sonia Boughattas
BACKGROUND: In the last decades, the enormous influx of immigrants to industrialized countries has led to outbreaks of parasitic diseases, with enteric infections being amongst the most frequently encountered. In its strategy to control such infection, Qatar has established the Pre-Employment Certificate (PEC) program which requires medical inspection before arrival in Qatar and which is mandatory for immigrant workers travelling to the country. To assess the reliability of the PEC, we conducted a survey of intestinal parasites, based on examination of stool samples provided by immigrant workers (n = 2,486) recently arrived in Qatar...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27892847/international-adoption-from-ethiopia-an-overview-of-the-health-status-at-arrival-in-belgium
#4
Lydia Van Kesteren, Marek Wojciechowski
BACKGROUND: Ethiopia is a densely populated country with a fast growing economy. Still socioeconomic and health issues render many children parentless. One thousand and twenty eight Ethiopian children have been adopted in Belgium from September 2005 to September 2015. Little has been published about their health status at arrival. METHODS: Three hundred and fifteen children adopted from Ethiopia were clinically evaluated at the Institute of Tropical Medicine in Antwerp from 1 January 2008 until 31 December 2014...
November 28, 2016: Acta Clinica Belgica
https://www.readbyqxmd.com/read/27891182/case-control-study-of-diarrheal-disease-etiology-in-individuals-over-5%C3%A2-years-in-southwest-china
#5
Shun-Xian Zhang, Chun-Li Yang, Wen-Peng Gu, Lin Ai, Emmanuel Serrano, Pin Yang, Xia Zhou, Shi-Zhu Li, Shan Lv, Zhi-Sheng Dang, Jun-Hu Chen, Wei Hu, Li-Guang Tian, Jia-Xu Chen, Xiao-Nong Zhou
BACKGROUND: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. METHODS: 271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27884121/prevalence-and-risk-factors-of-intestinal-protozoan-infections-a-population-based-study-in-rural-areas-of-boyer-ahmad-district-southwestern-iran
#6
Bahador Sarkari, Ghasem Hosseini, Mohammad Hossein Motazedian, Mohammad Fararouei, Abdolali Moshfe
BACKGROUND: Parasitic infections are still a significant health problem in rural areas in developing countries including Iran. There is no recent population-based data about the prevalence of human intestinal parasites in most rural areas of Iran. The current study aimed to determine the prevalence of intestinal protozoan infection in inhabitants of rural areas of Boyer-Ahmad district, Southwestern Iran. METHODS: A total of 1025 stool samples were collected from the inhabitant of 50 randomly selected villages in Boyer-Ahmad Township...
November 25, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27861635/multiplex-real-time-pcr-assay-targeting-eight-parasites-customized-to-the-korean-population-potential-use-for-detection-in-diarrheal-stool-samples-from-gastroenteritis-patients
#7
Eun Jeong Won, Soo Hyun Kim, Seung Jung Kee, Jong Hee Shin, Soon Pal Suh, Jong Yil Chai, Dong Wook Ryang, Myung Geun Shin
Intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. We developed a multiplex real-time PCR assay for detection of eight intestinal parasites and prospectively evaluated it for patients with gastroenteritis. The assay targeted Cryptosporidium parvum, Giardia lamblia, Entamoeba histolytica, Blastocystis hominis, Dientamoeba fragilis, Clonorchis sinensis, Metagonimus yokogawai, and Gymnophalloides seoi...
2016: PloS One
https://www.readbyqxmd.com/read/27858971/molecular-identification-and-subtype-analysis-of-blastocystis
#8
C Rune Stensvold, C Graham Clark
Several typing methods have been used in studies aiming to unravel the molecular epidemiology of Blastocystis, which is one of the most common intestinal parasites in human and many non-human hosts. Such studies have the potential to add to knowledge on Blastocystis transmission, host specificity, phylogeography, and clinical and public health significance, but rely on robust, standardized methods by which data can be generated and compared directly between studies. One of the most used methods is "barcoding,", which involves single-round PCR amplification and sequencing of partial small subunit ribosomal RNA genes of the parasites...
November 18, 2016: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/27858970/blastocystis-isolation-xenic-cultivation-and-cryopreservation
#9
C Graham Clark, C Rune Stensvold
Blastocystis is an intestinal parasite that is very easily isolated in culture from fresh stool samples. In fact, the parasite grows so readily in culture that short-term in vitro culture is sometimes used as a diagnostic tool in the absence of DNA-based methods. While axenizing Blastocystis cultures remains a significant challenge, the parasite can be propagated for several months in the presence of metabolically active bacteria (xenic culture). Hence, culture can be used for maintaining live Blastocystis strain libraries...
November 18, 2016: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/27854317/modulating-the-gut-micro-environment-in-the-treatment-of-intestinal-parasites
#10
REVIEW
Luis Vitetta, Emma Tali Saltzman, Tessa Nikov, Isabelle Ibrahim, Sean Hall
The interactions of micro-organisms cohabitating with Homo sapiens spans millennia, with microbial communities living in a symbiotic relationship with the host. Interacting to regulate and maintain physiological functions and immunological tolerance, the microbial community is able to exert an influence on host health. An example of micro-organisms contributing to an intestinal disease state is exhibited by a biodiverse range of protozoan and bacterial species that damage the intestinal epithelia and are therefore implicated in the symptoms of diarrhea...
November 16, 2016: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/27847411/zoonotic-intestinal-protozoan-of-the-wild-boars-sus-scrofa-in-persian-gulf-s-coastal-area-bushehr-province-southwestern-iran
#11
Kambiz Yaghoobi, Bahador Sarkari, Majid Mansouri, Mohammad Hossein Motazedian
AIM: Wild boars, Sus scrofa, are potential reservoirs of many zoonotic diseases, and there are a possibility of transmission of the zoonotic diseases from these animals to humans and also domestic animals. This study aimed to evaluate the protozoan contamination of wild boars in the Persian Gulf's coastal area (Bushehr Province), southwestern Iran. MATERIALS AND METHODS: A total of 25 crossbred boars were collected during a course of vertebrate pest control in Bushehr province, in 2013...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27811175/blastocystis-mitochondrial-genomes-appear-to-show-multiple-independent-gains-and-losses-of-start-and-stop-codons
#12
Alison S Jacob, Lee O'Brien Andersen, Paulina Pavinski Bitar, Vincent P Richards, Sarah Shah, Michael J Stanhope, C Rune Stensvold, C Graham Clark
Complete mitochondrion-related organelle (MRO) genomes of several subtypes (STs) of the unicellular stramenopile Blastocystis are presented. Complete conservation of gene content and synteny in gene order is observed across all MRO genomes, comprising 27 protein coding genes, 2 ribosomal RNA genes and 16 transfer RNA (tRNA) genes. Despite the synteny, differences in the degree of overlap between genes was observed between subtypes and also between isolates within the same subtype. Other notable features include unusual base-pairing mismatches in the predicted secondary structures of some tRNAs...
November 3, 2016: Genome Biology and Evolution
https://www.readbyqxmd.com/read/27783291/characteristics-of-factors-of-protozoa-blastocystis-hominis-persistence
#13
N I Potaturkina-Nesterova, N A Il'ina, N V Bugero, A S Nesterov
Persistence activity manifested in the expression of anti-lysozyme, anti-lactoferrin, and antihistone factors promoting inactivation of natural anti-infection resistance factors in the body was revealed in Blastocystis hominis protozoa. Activities of these factors were ranged. The frequency of these factors in clinical isolates of blastocyst decreased in the following order: anti-lactoferrin activity (84.5±3.7%)→anti-lysozyme activity (64.8±5.7%)→anti-histone activity (48.1±2.3%). In healthy humans, the corresponding parameters were 7...
October 26, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27770759/subtype-analysis-of-blastocystis-sp-isolates-from-asymptomatic-individuals-in-an-urban-community-in-the-philippines
#14
Davin Edric V. Adao, Ace O. Dela Serna, Maria Luz B. Belleza, Nicole R. Bolo, Windell L. Rivera
Blastocystis sp. is a commonly reported enteric protistan parasite in faecal specimens with a worldwide distribution afflicting both humans and a wide range of animals. The aim of this study is to characterize the subtypes (STs) of Blastocystis sp. isolates from asymptomatic individuals in an urban community in Pateros, Metro Manila, Philippines. The 600-bp small subunit ribosomal RNA (SSU rRNA) barcoding region of Blastocystis sp. isolates was amplified and sequenced using the primers RD5 and BhRDr. Subtypes were identified by uploading the sequences onto the Basic Local Alignment and Search Tool (BLAST) websites, the Blastocystis Subtype (18S) and Sequence Typing (MLST) Database and by construction of a phylogenetic tree...
October 1, 2016: Annals of Parasitology
https://www.readbyqxmd.com/read/27761331/occurrence-of-blastocystis-sp-in-water-catchments-at-malay-villages-and-aboriginal-settlement-during-wet-and-dry-seasons-in-peninsular-malaysia
#15
Samseh Abdullah Noradilah, Ii Li Lee, Tengku Shahrul Anuar, Fatmah Md Salleh, Siti Nor Azreen Abdul Manap, Noor Shazleen Husnie Mohd Mohtar, Syed Muhamad Azrul, Wan Omar Abdullah, Norhayati Moktar
In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1-K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages...
2016: PeerJ
https://www.readbyqxmd.com/read/27742000/editorial-par-int-special-issue-on-blastocystis
#16
Funda Dogruman-Al, Christen Rune Stensvold, Hisao Yoshikawa
No abstract text is available yet for this article.
December 2016: Parasitology International
https://www.readbyqxmd.com/read/27741951/epidemiological-and-clinical-profile-of-adult-patients-with-blastocystis-sp-infection-in-barcelona-spain
#17
Fernando Salvador, Elena Sulleiro, Adrián Sánchez-Montalvá, Carmen Alonso, Javier Santos, Isabel Fuentes, Israel Molina
BACKGROUND: Blastocystis spp. are among the most frequently observed intestinal parasites in humans. Despite the discovery of Blastocystis approximately 100 years ago, limited information is available regarding its pathogenesis, genetic diversity, and available treatment options. The aim of this study was to describe the epidemiological and clinical characteristics of patients with Blastocystis sp. infections diagnosed at Vall d'Hebron University Hospital (Barcelona, Spain). METHODS: A retrospective observational study was performed which included all adult patients who attended Vall d'Hebron University Hospital from February 2009 to March 2014 that had Blastocystis sp...
October 14, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27725159/subtype-analysis-of-blastocystis-isolates-using-ssu-rrna-dna-sequencing-in-rural-and-urban-population-in-southern-turkey
#18
Ismail Soner Koltas, Fadime Eroglu
Blastocystis is a common and emerging parasite often seen in many studies conducted in urban population, with scanty reports on rural communities. However, little is known about the public health significance of Blastocystis infection. A total of 28 Blastocystis isolates from 17 (17/28, 60.71%) patients living in rural area and 11 (11/28, 39.29%) patients living in urban area were screened with seven kinds of sequenced-tagged site primers for identification of subtype. PCR products were sequenced with same combination of primers using the BigDye Terminator V 3...
October 7, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27716755/-frequency-and-in-vitro-susceptibility-antiparasitic-of-blastocystis-hominis-from-patients-admitted-to-the-hospital-regional-lambayeque-peru
#19
Heber Silva-Díaz, Lorena Flores-Esqueche, Dunalia Llatas-Cancino, Génesis Guevara Vásquez, Teresa Silva-García
OBJECTIVE: To describe the frequency and antiparasitic in vitro susceptibility of Blastocystis hominis in patients admitted to theHospital Regional Lambayeque, Peru. MATERIAL AND METHODS: A cross-sectional study was conducted from January to August 2015 at 313 patients of all ages. B. hominis detection was performed on serial fecal samples by direct microscopic examination and microculture in modified Locke solution. The in vitro susceptibility testing against the drug metronidazole, nitazoxanide, trimethoprim-sulfamethoxazole and erythromycin was performed in 24 strains of B...
July 2016: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/27694739/gut-microbiota-related-to-giardia-duodenalis-entamoeba-spp-and-blastocystis-hominis-infections-in-humans-from-c%C3%A3-te-d-ivoire
#20
Valerio Iebba, Floriana Santangelo, Valentina Totino, Fabrizio Pantanella, Anatole Monsia, Veronica Di Cristanziano, David Di Cave, Serena Schippa, Federica Berrilli, Rossella D'Alfonso
INTRODUCTION: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d'Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. METHODOLOGY: Twenty fecal samples were collected from people inhabiting three different localities of Côte d'Ivoire for copromicroscopic analysis and molecular identification of G...
September 30, 2016: Journal of Infection in Developing Countries
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