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https://www.readbyqxmd.com/read/28743157/her2-mutations-in-lung-adenocarcinomas-a-report-from-the-lung-cancer-mutation-consortium
#1
Rathi N Pillai, Madhusmita Behera, Lynne D Berry, Mike R Rossi, Mark G Kris, Bruce E Johnson, Paul A Bunn, Suresh S Ramalingam, Fadlo R Khuri
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) mutations have been reported in lung adenocarcinomas. Herein, the authors describe the prevalence, clinical features, and outcomes associated with HER2 mutations in 1007 patients in the Lung Cancer Mutation Consortium (LCMC). METHODS: Patients with advanced-stage lung adenocarcinomas were enrolled to the LCMC. Tumor specimens were assessed for diagnosis and adequacy; multiplexed genotyping was performed in Clinical Laboratory Improvement Amendments (CLIA)-certified laboratories to examine 10 oncogenic drivers...
July 25, 2017: Cancer
https://www.readbyqxmd.com/read/28743122/utility-of-transbronchial-lung-cryobiopsy-in-non-interstitial-diseases
#2
Olivia Sánchez-Cabral, Dina Martínez-Mendoza, Sebastián Fernandez-Bussy, Berenice López-González, Carolina Perea-Talamantes, Rosa María Rivera-Rosales, César Luna-Rivero, José Arturo Martínez-Orozco, Luis Felipe Flores-Suárez, Patricio Santillán-Doherty, Gustavo Reyes-Terán
BACKGROUND: Transbronchial lung cryobiopsy (TLCB), performed with a flexible cryoprobe, is an interventional pulmonology procedure that has proved its diagnostic value for interstitial pulmonary disease. However, it has not been explored extensively as a diagnostic tool for patients with non-interstitial lung pathology, including infectious and malignant diseases. OBJECTIVE: To evaluate the diagnostic yield and safety of an interventional pulmonology approach that integrates TLCB and bronchoalveolar lavage (BAL) for the diagnosis of non-interstitial pulmonary disease...
July 26, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/28743115/cerebrospinal-fluid-levels-of-14-3-3-gamma-what-does-it-tell-us-about-sporadic-creutzfeldt-jakob-disease
#3
Christian Humpel, Thomas Benke
Clinical diagnosis of Creutzfeldt-Jakob disease (CJD) can be supported by the analysis of Tau and 14-3-3 in the cerebrospinal fluid (CSF). In this short report, we report about a retrospective analysis performed on 2,296 routinely collected CSF samples, and 44 samples with a ratio of phosphoTau181/Tau <0.075 were selected. Analysis was performed with a novel 14-3-3 gamma CircuLex Elisa. We show that control levels were around 6,000 AU/mL and samples from Alzheimer patients were not different from those collected from healthy controls...
July 26, 2017: Pharmacology
https://www.readbyqxmd.com/read/28743082/characterization-and-evaluation-of-nanoencapsulated-diethylcarbamazine-in-model-of-acute-hepatic-inflammation
#4
Gabriel Barros Rodrigues, Elquio Eleamen Oliveira, Francisco Jaime Bezerra Mendonça Junior, Laise Aline Martins Dos Santos, Wilma Helena de Oliveira, Maria Eduarda Rocha de França, Deniele Bezerra Lós, Brennda Martins Gabínio, Fábia Cristiane Melo Leite de Lira, Christina Alves Peixoto
Previous studies from our laboratory have demonstrated that Diethylcarbamazine (DEC) is a potent anti-inflammatory drug. The aim of the present study was to characterize the nanoencapsulation of DEC and to evaluate its effectiveness in a model of inflammation for the first time. C57BL/6 mice were divided into six groups: 1) Control; 2) Carbon tetrachloride (CCl4); 3) DEC 25mg/kg+CCl4; 4) DEC 50mg/kg+CCl4; 5) DEC-NANO 05mg/kg+CCl4 and 6) DEC-NANO 12.5mg/kg+CCl4. Liver fragments were stained with hematoxylin-eosin, and processed for Western blot, ELISA and immunohistochemistry...
July 22, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28743073/in-situ-biodegradation-potential-of-1-2-dca-and-vc-at-sites-with-different-hydrogeological-settings
#5
R C M Nobre, M M M Nobre, T M P Campos, D Ogles
This paper investigates the feasibility of applying in-situ Bioremediation (ISB) to three sites contaminated with vinyl chloride and/or chlorinated alkanes such as 1,2-DCA and 1,1,2-TCA, presenting distinct hydrogeological settings and history of contaminant loading. Biotransformation of these compounds is well established in laboratory studies and pure cultures. Due to confidential aspects, however, few field data are available to support real case studies to the predictability of their fate and lifetime in soil and groundwater...
July 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28743069/the-influence-of-134-cs-on-the-137-cs-gamma-spectrometric-peak-to-valley-ratio-and-improvement-of-the-peak-to-valley-method-by-limiting-the-detector-field-of-view
#6
Karl Östlund, Christer Samuelsson, Sören Mattsson, Christopher L Rääf
The peak-to-valley method was investigated under laboratory conditions and in situ with respect to both (134)Cs perturbation of the (137)Cs valley and use of collimation. The (134)Cs perturbation is significant down to (134)Cs:(137)Cs activity ratios of 1:100. In these cases the full energy peaks from (134)Cs (796 and 802keV) and associated valley should be used instead of the peak and valley from the (137)Cs 662keV peak. Use of collimators in situ outside Fukushima Daiichi significantly increased PTV for (134)Cs...
July 15, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28743065/enantiomerization-and-stereoselectivity-in-bioaccumulation-of-furalaxyl-in-tenebrio-molitor-larvae
#7
Jing Yin, Yongxin Gao, Feilong Zhu, Weiyu Hao, Qi Xu, Huili Wang, Baoyuan Guo
Furalaxyl is a chiral pesticide and widely used in modern agriculture as racemate mixture. The enantiomerization and enantioselecive bioaccumulation by a single dose of furalaxyl to Tenebrio molitor larvae under laboratory conditions were studied using a high-performance liquid chromatography tandem mass spectroscopy method based on a ChiralPAK IC column. Our results showed that a significant enantiomerization (interconversion between R-enantiomer and S-enantiomer) was observed in Tenebrio molitor larvae under R- or S-furalaxyl exposure...
July 22, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28743024/a-method-to-evaluate-the-quality-of-clinical-gene-panel-sequencing-data-for-single-nucleotide-variant-detection
#8
Chung Lee, Joon S Bae, Gyu H Ryu, Nayoung K D Kim, Donghyun Park, Jongsuk Chung, Sungkyu Kyung, Je-Gun Joung, Hyun-Tae Shin, Seung-Ho Shin, Younglan Kim, Byung S Kim, Hojun Lee, Kyoung-Mee Kim, Jung-Sun Kim, Woong-Yang Park, Dae-Soon Son
Customized gene-panel tests, based on next-generation sequencing, have demonstrated their usefulness in a plethora of clinical settings. As with other clinical diagnostic techniques, gene-panel sequencing for clinical purposes requires precise quality control (QC) measures to ensure its reliability. Only detected variants are currently recorded in clinical reports; however, identifying whether a nondetected variant is a true or false negative is regarded essential in a clinical setting and, thus, a comprehensive QC measure is in demand...
July 22, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28742969/fabrication-of-a-new-lineage-of-artificial-luciferases-from-natural-luciferase-pools
#9
Sung Bae Kim, Ryo Nishihara, Daniel Citterio, Koji Suzuki
The fabrication of artificial luciferases (ALucs) with unique optical properties can have a fundamental impact on bioassays and molecular imaging. In this study, we developed a new lineage of ALucs with unique substrate preferences by extracting consensus amino acids from the alignment of 25 copepod luciferase sequences available in published databases. The primary sequence was first created with a sequence logo generator resulting in a total of 11 sibling sequences. Phylogenetic analysis shows that the newly fabricated ALucs form an independent branch, genetically isolated from the natural luciferases and from a prior series of ALucs produced by our laboratory using a smaller basis set...
July 25, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28742897/domestic-correspondence-laboratory-work-in-medical-schools
#10
(no author information available yet)
No abstract text is available yet for this article.
July 25, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28742877/predictors-of-mortality-in-autoimmune-disease-patients-with-concurrent-cytomegalovirus-infections-detected-by-quantitative-real-time-pcr
#11
Kyoung Yong Lee, Byung-Woo Yoo, Sung Soo Ahn, William Han Bae, Hyukmin Lee, Seung Min Jung, Sang-Won Lee, Yong-Beom Park, Jason Jungsik Song
OBJECTIVE: Cytomegaloviruses (CMV) can have a significant impact on the prognosis of immunocompromised patients. Unlike in the transplantation and AIDS fields, only a few studies on CMV infections have been published in the field of autoimmunity. In this study, we examined the clinical outcomes of CMV infections in patients with autoimmune diseases at a single tertiary medical institution. METHODS: A retrospective study was performed to identify the mortality risk factors associated with CMV infections in patients with autoimmune diseases...
2017: PloS One
https://www.readbyqxmd.com/read/28742875/the-significance-of-inadequate-transcranial-doppler-studies-in-children-with-sickle-cell-disease
#12
Simon Greenwood, Colin Deane, Oliver L Rees, Ben Freedman, Suresh Kumar, Naser Ben Ramadan, Sarah Wilkinson, Grant Marais, Julie Lord, Subarna Chakravorty, Susan E Height, Kate Gardner, David C Rees
Sickle cell disease (SCD) is a common cause of cerebrovascular disease in childhood. Primary stroke prevention is effective using transcranial Doppler (TCD) scans to measure intracranial blood velocities, and regular blood transfusions or hydroxycarbamide when these are abnormal. Inadequate TCD scans occur when it is not possible to measure velocities in all the main arteries. We have investigated the prevalence and significance of this in a retrospective audit of 3915 TCD scans in 1191 children, performed between 2008 and 2015...
2017: PloS One
https://www.readbyqxmd.com/read/28742824/how-do-rehomed-laboratory-beagles-behave-in-everyday-situations-results-from-an-observational-test-and-a-survey-of-new-owners
#13
Dorothea Döring, Ophelia Nick, Alexander Bauer, Helmut Küchenhoff, Michael H Erhard
When laboratory dogs are rehomed into private households, they experience an extreme change in their life situation. They leave their familiar, limited environment in the research facility and encounter a multitude of animate and inanimate stimuli in their new home. Although literature reports have described the experiences with rehoming as being mostly positive, scientific observations of the dogs in everyday situations have not been done. Hence, we conducted an observational test with 74 laboratory beagles 6 weeks after adoption in their new homes...
2017: PloS One
https://www.readbyqxmd.com/read/28742818/first-identification-of-a-recombinant-form-of-hepatitis-c-virus-in-austrian-patients-by-full-genome-next-generation-sequencing
#14
Evelyn Stelzl, Bernhard Haas, Bernd Bauer, Sherry Zhang, Ellen H Fiss, Grantland Hillman, Aaron T Hamilton, Rochak Mehta, Marintha L Heil, Ed G Marins, Brigitte I Santner, Harald H Kessler
Hepatitis C virus (HCV) intergenotypic recombinant forms have been reported for various HCV genotypes/subtypes in several countries worldwide. In a recent study, four patients living in Austria had been identified to be possibly infected with a recombinant HCV strain. To clarify results and determine the point of recombination, full-genome next-generation sequencing using the Illumina MiSeq v2 300 cycle kit (Illumina, San Diego, CA, USA) was performed in the present study. Samples of all of the patients contained the recombinant HCV strain 2k/1b...
2017: PloS One
https://www.readbyqxmd.com/read/28742805/intra-dialytic-hypertension-is-associated-with-high-mortality-in-hemodialysis-patients
#15
Chi-Young Choi, Jae Seok Park, Kyu Tae Yoon, Hyo Wook Gil, Eun Young Lee, Sae Yong Hong
BACKGROUND: Intra-dialytic hypertension (IDH) is emerging as an important issue in hemodialysis patients. Its risk factors and clinical outcomes are unclear. METHODS: A total of 73 prevalent hemodialysis patients were enrolled. They included 14 (19.2%) patients with baseline IDH and 59 patients without IDH. Their clinical parameters, laboratory parameters, and mortality were investigated over 78 months. RESULTS: The risks factor of IDH included low serum potassium levels, low ultrafiltration, and low arm muscle area...
2017: PloS One
https://www.readbyqxmd.com/read/28742797/a-comparison-of-selective-aortic-arch-perfusion-and-resuscitative-endovascular-balloon-occlusion-of-the-aorta-for-the-management-of-hemorrhage-induced-traumatic-cardiac-arrest-a-translational-model-in-large-swine
#16
Ed B G Barnard, James E Manning, Jason E Smith, Jason M Rall, Jennifer M Cox, James D Ross
BACKGROUND: Survival rates remain low after hemorrhage-induced traumatic cardiac arrest (TCA). Noncompressible torso hemorrhage (NCTH) is a major cause of potentially survivable trauma death. Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) at the thoracic aorta (Zone 1) can limit subdiaphragmatic blood loss and allow for IV fluid resuscitation when intrinsic cardiac activity is still present. Selective Aortic Arch Perfusion (SAAP) combines thoracic aortic balloon hemorrhage control with intra-aortic oxygenated perfusion to achieve return of spontaneous circulation (ROSC) when cardiac arrest has occurred...
July 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28742723/supraglottic-atomization-of-surfactant-in-spontaneously-breathing-lambs-receiving-continuous-positive-airway-pressure
#17
Ilaria Milesi, David G Tingay, Anna Lavizzari, Federico Bianco, Emanuela Zannin, Paolo Tagliabue, Fabio Mosca, Maria Luisa Ventura, Anushi Rajapaksa, Elizabeth J Perkins, Don Black, Marco Di Castri, Magdy Sourial, Gerhard Pohlmann, Raffaele L Dellaca'
OBJECTIVES: To determine the short-term tolerance, efficacy, and lung deposition of supraglottic atomized surfactant in spontaneously breathing lambs receiving continuous positive airway pressure. DESIGN: Prospective, randomized animal study. SETTING: Animal research laboratory. SUBJECTS: Twenty-two preterm lambs on continuous positive airway pressure (132 ± 1 d gestational age). INTERVENTIONS: Animals receiving continuous positive airway pressure via binasal prongs at 8 cm H2O were randomized to receive atomized surfactant at approximately 60-minute of life (atom; n = 15) or not (control; n = 7)...
July 22, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28742636/the-influence-of-chloride-for-the-interpretation-of-plasma-bicarbonate-during-the-treatment-of-diabetic-ketoacidosis
#18
Juan Pablo Ferreira, Magali Hamui, Milagros Torrents, Romina Carrano, Mabel Ferraro, Ismael Toledo
INTRODUCTION: Hyperchloremic metabolic acidosis can occur in diabetic ketoacidosis (DKA) and may affect the acid-base interpretation during treatment. OBJECTIVES: This study aims to describe the prevalence of hyperchloremia during the treatment of DKA and its effect on the interpretation of bicarbonate value. METHODS: A cross-sectional study, including all cases of DKA in patients aged 1 to 18 years old admitted from 2010 to 2015, was performed...
July 24, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28742609/the-efficacy-of-dynamic-contract-relax-stretching-on-delayed-onset-muscle-soreness-among-healthy-individuals-a-randomized-clinical-trial
#19
Yanfei Xie, Beibei Feng, Kedi Chen, Lars L Andersen, Phil Page, Yuling Wang
OBJECTIVE: To evaluate the efficacy of dynamic contract-relax stretching on delayed-onset muscle soreness (DOMS) in the calf muscle of healthy individuals. DESIGN: Randomized clinical trial. SETTING: Research laboratory. PARTICIPANTS: Three groups of 16 healthy participants (n = 48) were recruited by convenience sampling. INTERVENTIONS: Three sets of resisted bilateral heel-raising exercises until exhaustion were conducted to initiate DOMS...
July 21, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/28742605/predictors-of-the-biomechanical-effects-of-customized-foot-orthoses-in-adults-with-flat-arched-feet
#20
John B Arnold, Thomas May, Christopher Bishop
OBJECTIVE: To determine the potential presence and characteristics of biomechanical responders to customized foot orthoses during walking in adults with flat-arched feet. DESIGN: Experimental, repeated-measures. SETTING: University clinic and laboratory. PARTICIPANTS: Eighteen symptom-free adults with flat-arched feet. INTERVENTIONS: Customized foot orthoses. MAIN OUTCOME MEASURES: In-shoe foot biomechanics were measured during walking with and without customized foot orthoses using 3D analysis...
July 21, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
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