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cadiomyocyte function

Alessandra Ghigo, Irene Franco, Fulvio Morello, Emilio Hirsch
Myocardial damage, by different noxious causes, triggers an inflammatory reaction driving post-injury repair mechanisms and chronic remodelling processes that are largely detrimental to cardiac function. Cardiomyocytes have recently emerged as key players in orchestrating this inflammatory response. Injured cardiomyocytes release damage-associated molecular pattern molecules, such as high-mobility group box 1 (HMGB1), DNA fragments, heat shock proteins, and matricellular proteins, which instruct surrounding healthy cadiomyocytes to produce inflammatory mediators...
May 1, 2014: Cardiovascular Research
A Pavesi, M Soncini, A Zamperone, S Pietronave, E Medico, A Redaelli, M Prat, G B Fiore
In tissue engineering, several factors play key roles in providing adequate stimuli for cells differentiation, in particular biochemical and physical stimuli, which try to mimic the physiological microenvironments. Since electrical stimuli are important in the developing heart, we have developed an easy-to-use, cost-effective cell culture platform, able to provide controlled electrical stimulation aimed at investigating the influence of the electric field in the stem cell differentiation process. This bioreactor consists of an electrical stimulator and 12 independent, petri-like culture chambers and a 3-D computational model was used to characterize the distribution and the intensity of the electric field generated in the cell culture volume...
July 2014: Biotechnology and Bioengineering
Li Wang, Li Qian
Numerous cardiac diseases, including myocardial infarction (MI) and chronic heart failure, have been associated with cardiomyocyte apoptosis. Promoting cell survival by inhibiting apoptosis is one of the effective strategies to attenuate cardiac dysfunction caused by cardiomyocyte loss. miR-24 has been shown as an anti-apoptotic microRNA in various animal models. In vivo delivery of miR-24 into a mouse MI model suppressed cardiac cell death, attenuated infarct size, and rescued cardiac dysfunction. However, the molecular pathway by which miR-24 inhibits cardiomyocyte apoptosis is not known...
2014: PloS One
Yong Li, Bingong Li, Zhe Li, Jian Zhang, Minghui Zeng
OBJECTIVE: To transfect rat adipose tissue-derived adult stem cells (ADSCs) with the pacemaker gene of human hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (hHCN2), and observe its feasibility of functioning as pacemaker cells. METHODS: First of all, healthy ADSCs were harvested and transfected with hHCN2 gene. Thereafter, we detected the expression of hHCN2 gene and protein by quantitative real-time PCR (qRT-PCR), Western blot analysis and immunofluorescence cytochemistry and measured the electrophysiological properties and recorded the inward currents using patch clamp techniques...
September 2013: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Hu Wang, Zhaohui Li, Jizheng Wang, Kai Sun, Qiqiong Cui, Lei Song, Yubao Zou, Xiaojian Wang, Xuan Liu, Rutai Hui, Yuxin Fan
Hypertrophic cardiomyopathy (HCM), the most common inherited cardiac disorder, is characterized by increased ventricular wall thickness that cannot be explained by underlying conditions, cadiomyocyte hypertrophy and disarray, and increased myocardial fibrosis. In as many as 50% of HCM cases, the genetic cause remains unknown, suggesting that more genes may be involved. Nexilin, encoded by NEXN, is a cardiac Z-disc protein recently identified as a crucial protein that functions to protect cardiac Z-discs from forces generated within the sarcomere...
November 12, 2010: American Journal of Human Genetics
E Holt, P K Lunde, O M Sejersted, G Christensen
A major limitation in long-term studies of quiescent adult cadiomyocytes in culture has been the decline in contractile properties of the cells over time. Regular contracting cardiomyocyte cultures may represent a more physiological model. The aim of the present study was to investigate the mechanical properties and calcium handling of myocytes after 24 hours of electrical stimulation at 1 Hz. In a random and blind design, stimulated (S) and unstimulated (U) myocytes were examined using an inverted microscope which allows continuous length recordings and measurements of intracellular Ca2+...
October 1997: Basic Research in Cardiology
B Lewartowski, B Wolska
The effect of Thapsigargin (TG) shown to block selectively the Ca(2+)-ATPase of sarcoplasmic reticulum (SR) on the SR Ca2+ content and contractions of single rat cadiomyocytes was investigated. In 80% of cells 10(-6) M TG blocked Ca2+ uptake by SR at rest, depleted the SR Ca2+, inhibited postrest potentiation and changed the pattern of response to premature contractions. Amplitude of steady state contractions was reduced to 11 +/- 4% of pre-TG control. In 20% of cells TG had similar effect on the SR function, however, amplitude of contractions was reduced to only 50 +/- 7% of control...
September 1993: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
S S Peng, M Y Zhang, Y Z Zhang, Z H Wu
Rats of both sexes were fed on a basal feed containing 1% refined konjac meal (RKM) for 18 months and the effects of RKM on the cell aging were observed. A comparable group fed on the basic feed was used as the control. Results obtained demonstrate that the long-term feeding of RKM to rats can delay the course of cell aging of the gliocyte, cadiomyocyte, and the endothelial cell of the large and medium arteriases, hence it is likely to delay the occurrence of arteriosclerosis and improve the functions of the brain, heart and vascular system...
March 1995: Biomedical and Environmental Sciences: BES
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