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https://www.readbyqxmd.com/read/27922086/photoelectrochemical-detection-of-alpha-fetoprotein-based-on-zno-inverse-opals-structure-electrodes-modified-by-ag2s-nanoparticles
#1
Yandong Jiang, Dali Liu, Yudan Yang, Ru Xu, Tianxiang Zhang, Kuang Sheng, Hongwei Song
In this work, a new photoelectrochemical biosensor based on Ag2S nanoparticles (NPs) modified macroporous ZnO inverse opals structure (IOs) was developed for sensitive and rapid detection of alpha fetal protein (AFP). Small size and uniformly dispersed Ag2S NPs were prepared using the Successive Ionic Layer Adsorption And Reaction (SILAR) method, which were adsorbed on ZnO IOs surface and frame work as matrix for immobilization of AFP. The composite structure of ZnO/Ag2S expanded the scope of light absorption to long wavelength, which can make full use of the light energy...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921033/new-tools-to-study-contact-activation
#2
Steffen Rosén
The recent availability of a sensitive chromogenic method approach for determination of FXIa activity has been explored for designing sensitive methods for FXIIa and kallikrein, both using FXa formation as the read-out. For both enzymes the assay range 1-10 nmol/L provides a resolution of about 0.8 absorbance units with a total assay time of about 20 min. For studies on activation kinetics, subsampling and extensive dilution can be performed in MES-bovine serum albumin (BSA) buffer pH 5.7 for quenching of enzyme activity and with ensuing determination of FXa generation in a chromogenic FXIa method...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27920766/a-degu-p-and-degq-dependent-regulatory-pathway-for-the-k-state-in-bacillus-subtilis
#3
Mathieu Miras, David Dubnau
The K-state in the model bacterium Bacillus subtilis is associated with transformability (competence) as well as with growth arrest and tolerance for antibiotics. Entry into the K-state is determined by the stochastic activation of the transcription factor ComK and occurs in about ∼15% of the population in domesticated strains. Although the upstream mechanisms that regulate the K-state have been intensively studied and are well understood, it has remained unexplained why undomesticated isolates of B. subtilis are poorly transformable compared to their domesticated counterparts...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27919702/design-of-pvp-va-s-630-based-tadalafil-solid-dispersion-to-enhance-the-dissolution-rate
#4
Jin-Seok Choi, Jeong-Sook Park
Tadalafil (TDF) is a Biopharmaceutics Classification System (BCS) class II drug; the efficacy thereof is critically limited by inherent poor water solubility. Solid dispersion (SD) techniques are widely used to improve the bioavailability of drugs that are poorly water-soluble. Herein, we used an SD technique to improve the solubility and in vitro dissolution rate of TDF; a solvent evaporation method was applied involving the use of hydrophilic carriers (PVP/VA S-630) and assistants (malic acid or meglumine)...
December 2, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27919657/synthesis-of-novel-steroidal-agonists-partial-agonists-and-antagonists-for-the-glucocorticoid-receptor
#5
Zhuang Jin, Hua Lin, Sathish Srinivasan, Jerome C Nwachukwu, Nelson Bruno, Patrick R Griffin, Kendall W Nettles, Theodore M Kamenecka
Adverse effects of glucocorticoids could be limited by developing new compounds that selectively modulate anti-inflammatory activity of the glucocorticoid receptor (GR). We have synthesized a novel series of steroidal GR ligands, including potent agonists, partial agonists and antagonists with a wide range of effects on inhibiting secretion of interleukin-6. Some of these new ligands were designed to directly impact conformational stability of helix-12, in the GR ligand-binding domain (LBD). These compounds modulated GR activity and glucocorticoid-induced gene expression in a manner that was inversely correlated to the degree of inflammatory response...
November 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27919629/pre-clinical-development-of-a-hydrogen-peroxide-inactivated-west-nile-virus-vaccine
#6
Elizabeth A Poore, Dawn K Slifka, Hans-Peter Raué, Archana Thomas, Erika Hammarlund, Benjamin K Quintel, Lindsay L Torrey, Ariel M Slifka, Justin M Richner, Melissa E Dubois, Lawrence P Johnson, Michael S Diamond, Mark K Slifka, Ian J Amanna
West Nile virus (WNV) is a mosquito-transmitted pathogen with a wide geographical range that can lead to long-term disability and death in some cases. Despite the public health risk posed by WNV, including an estimated 3 million infections in the United States alone, no vaccine is available for use in humans. Here, we present a scaled manufacturing approach for production of a hydrogen peroxide-inactivated whole virion WNV vaccine, termed HydroVax-001WNV. Vaccination resulted in robust virus-specific neutralizing antibody responses and protection against WNV-associated mortality in mice or viremia in rhesus macaques (RM)...
December 2, 2016: Vaccine
https://www.readbyqxmd.com/read/27919603/preparation-of-quantum-dots-cdte-decorated-graphene-composite-for-sensitive-detection-of-uric-acid-and-dopamine
#7
Hong-Wei Yu, Jing-Hui Jiang, Ze Zhang, Guang-Cai Wan, Zhi-Yong Liu, Dong Chang, Hong-Zhi Pan
The assembly of quantum dots (QDs) in a simply method opens up opportunities to obtain access to the full potential of assembled QDs by virtue of the collective properties of the ensembles. In this study, quantum dots CdTe and graphene (Gr) nanocomposite was constructed for the simultaneous determination of uric acid (UA) and dopamine (DA). The CdTe QDs-Gr nanocomposite was prepared by ultrasonication and was characterized with microscopic techniques. The nanocomposite modified electrode was characterized by cyclicvoltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS)...
December 2, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27919215/current-strategies-in-modification-of-plga-based-gene-delivery-system
#8
Mohammad Ramezani, Mahboubeh Ebrahimian, Maryam Hashemi
The successful gene therapy has been limited by safe and efficient delivery of nucleic acid to the target cells. Poly (d,l-lactide-co-glycolide) (PLGA) nanoparticles (NPs) are able to deliver drugs and gene efficiently. This formulation has several advantages in comparison with other formulations including improvement of solubility, stability, controlling of degradation and release of the entrapped agents. For application of PLGA as gene carrier, there exist many challenges. PLGA nanoparticles could protect the encapsulated DNA from in vivo degradation but the DNA release is slowl and their negative charge acts as a barrier to DNA incorporation and delivery...
December 5, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27919108/biocatalytic-synthesis-of-flavonoid-esters-by-lipases-and-their-biological-benefits
#9
Maria Elisa M B de Araújo, Yollanda E M Franco, Marcia C F Messias, Giovanna B Longato, João A Pamphile, Patricia de O Carvalho
Several studies have described important biological activities of flavonoids such as coronary heart disease prevention, hepatoprotective, anti-inflammatory and anticancer activities, enzyme inhibition activity, and antibacterial, antifungal, and antiviral activities. Flavonoids show promising activity as natural plant-based antioxidants due to their antioxidant and free radical scavenging properties. However, their primary applications as antioxidants in the pharmaceutical, cosmetic, and food industries are limited because of their moderately hydrophilic nature...
December 5, 2016: Planta Medica
https://www.readbyqxmd.com/read/27918748/the-cxcl12-cxcr4-signaling-pathway-a-new-susceptibility-factor-in-human-papillomavirus-pathogenesis
#10
Floriane Meuris, Laetitia Carthagena, Agnieszka Jaracz-Ros, Françoise Gaudin, Pasquale Cutolo, Claire Deback, Yuezhen Xue, Françoise Thierry, John Doorbar, Françoise Bachelerie
The productive human papillomavirus (HPV) life cycle is tightly linked to the differentiation and cycling of keratinocytes. Deregulation of these processes and stimulation of cell proliferation by the action of viral oncoproteins and host cell factors underlies HPV-mediated carcinogenesis. Severe HPV infections characterize the wart, hypogammaglobulinemia, infection, and myelokathexis (WHIM) immunodeficiency syndrome, which is caused by gain-of-function mutations in the CXCR4 receptor for the CXCL12 chemokine, one of which is CXCR41013...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27918568/solution-based-electrical-doping-of-semiconducting-polymer-films-over-a-limited-depth
#11
Vladimir A Kolesov, Canek Fuentes-Hernandez, Wen-Fang Chou, Naoya Aizawa, Felipe A Larrain, Ming Wang, Alberto Perrotta, Sangmoo Choi, Samuel Graham, Guillermo C Bazan, Thuc-Quyen Nguyen, Seth R Marder, Bernard Kippelen
Solution-based electrical doping protocols may allow more versatility in the design of organic electronic devices; yet, controlling the diffusion of dopants in organic semiconductors and their stability has proven challenging. Here we present a solution-based approach for electrical p-doping of films of donor conjugated organic semiconductors and their blends with acceptors over a limited depth with a decay constant of 10-20 nm by post-process immersion into a polyoxometalate solution (phosphomolybdic acid, PMA) in nitromethane...
December 5, 2016: Nature Materials
https://www.readbyqxmd.com/read/27918297/a-novel-one-dimensional-double-chain-like-zn-ii-coordination-polymer-poly-bis-1-benzyl-1h-imidazole-%C3%AE%C2%BAn-3-tris-%C3%AE-cyanido-%C3%AE%C2%BA-2-c-n-cyanido-%C3%AE%C2%BAc-disilver-i-zinc-ii
#12
Pattiya Krinchampa, Kittipong Chainok, Siriporn Phengthaisong, Sujittra Youngme, Filip Kielar, Nanthawat Wannarit
One of most interesting systems of coordination polymers constructed from the first-row transition metals is the porous Zn(II) coordination polymer system, but the numbers of such polymers containing N-donor linkers are still limited. The title double-chain-like Zn(II) coordination polymer, [Ag2Zn(CN)4(C10H10N2)2]n, presents a one-dimensional linear coordination polymer structure in which Zn(II) ions are linked by bridging anionic dicyanidoargentate(I) units along the crystallographic b axis and each Zn(II) ion is additionally coordinated by a terminal dicyanidoargentate(I) unit and two terminal 1-benzyl-1H-imidazole (BZI) ligands, giving a five-coordinated Zn(II) ion...
December 1, 2016: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/27918182/theory-guided-exploration-with-structural-equation-model-forests
#13
Andreas M Brandmaier, John J Prindle, John J McArdle, Ulman Lindenberger
Structural equation model (SEM) trees, a combination of SEMs and decision trees, have been proposed as a data-analytic tool for theory-guided exploration of empirical data. With respect to a hypothesized model of multivariate outcomes, such trees recursively find subgroups with similar patterns of observed data. SEM trees allow for the automatic selection of variables that predict differences across individuals in specific theoretical models, for instance, differences in latent factor profiles or developmental trajectories...
December 2016: Psychological Methods
https://www.readbyqxmd.com/read/27918142/anodized-aluminum-oxide-templated-synthesis-of-metal-organic-frameworks-used-as-membrane-reactors
#14
Yifu Yu, Xue-Jun Wu, Meiting Zhao, Qinglang Ma, Junze Chen, Bo Chen, Melinda Sindoro, Jian Yang, Shikui Han, Qipeng Lu, Hua Zhang
The incorporation of metal-organic frameworks (MOFs) into membrane-shaped architectures is of great importance for practical applications. The currently synthesized MOF-based membranes show many disadvantages, such as poor compatibility, low dispersity, and instability, which severely limit their utility. Herein, we present a general, facile, and robust approach for the synthesis of MOF-based composite membranes through the in situ growth of MOF plates in the channels of anodized aluminum oxide (AAO) membranes...
December 5, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27917560/is-best-worst-scaling-suitable-for-health-state-valuation-a-comparison-with-discrete-choice-experiments
#15
Nicolas Krucien, Verity Watson, Mandy Ryan
Health utility indices (HUIs) are widely used in economic evaluation. The best-worst scaling (BWS) method is being used to value dimensions of HUIs. However, little is known about the properties of this method. This paper investigates the validity of the BWS method to develop HUI, comparing it to another ordinal valuation method, the discrete choice experiment (DCE). Using a parametric approach, we find a low level of concordance between the two methods, with evidence of preference reversals. BWS responses are subject to decision biases, with significant effects on individuals' preferences...
December 4, 2016: Health Economics
https://www.readbyqxmd.com/read/27916894/green-synthesis-of-silver-nanoparticles-stabilized-with-mussel-inspired-protein-and-colorimetric-sensing-of-lead-ii-and-copper-ii-ions
#16
Ja Young Cheon, Won Ho Park
This articles reports a simple and green method for preparing uniform silver nanoparticles (AgNPs), for which self-polymerized 3,4-dihydroxy-l-phenylalanine (polyDOPA) is used as the reducing and stabilizing agent in aqueous media. The AgNPs functionalized by polyDOPA were analyzed by UV-Vis spectroscopy, high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), Raman spectrophotometry, and X-ray diffraction (XRD) techniques...
November 30, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916881/optimization-of-cyanine-dye-stability-and-analysis-of-fret-interaction-on-dna-microarrays
#17
Marcel von der Haar, Christopher Heuer, Martin Pähler, Kathrin von der Haar, Patrick Lindner, Thomas Scheper, Frank Stahl
The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores' susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS)...
November 30, 2016: Biology
https://www.readbyqxmd.com/read/27916553/nio-hedgehog-like-nanostructures-au-polyaniline-nanofibers-reduced-graphene-oxide-nanocomposite-with-electrocatalytic-activity-for-non-enzymatic-detection-of-glucose
#18
Kh Ghanbari, F Ahmadi
A novel sensitive non-enzymatic glucose sensor was fabricated based on nickel oxide hedgehog-like nanostructures decorated ternary gold nanoparticles/polyaniline nanofibers/reduced graphene oxide nanocomposites (NiO/Au/PANI/rGO). The morphology and structure of NiO/Au/PANI/rGO nanocomposites were characterized by field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Raman spectroscopy, and Fourier transform infrared (FT-IR). Electrochemical impedance spectroscopy (EIS) data also indicated the charge transfer of each layer decreased compared to the underneath layer...
December 1, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27916403/stability-of-synthetic-cathinones-in-oral-fluid-samples
#19
Briana Miller, Jiyoung Kim, Marta Concheiro
Synthetic cathinones are new stimulant drugs derived from cathinone that have been sold as "legal highs" worldwide. These compounds can elicit powerful effects such as delusions, hallucinations as well as other potentially dangerous behavior. New analogs with varying effects and potencies are constantly introduced in the market to evade legislation, and they are not detected by routine screening and confirmation methods. Oral fluid is an alternative matrix of increasing interest in forensic toxicology. Its collection is non-invasive and easily supervised, and positive drug findings typically reflect recent drug exposure...
December 1, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27916190/editorial-commentary-natural-history-of-shoulder-dislocation-is-recurrence-our-only-metric
#20
EDITORIAL
Brett D Owens
The natural history of a traumatic anterior glenohumeral dislocation has been the subject of significant study, often with differing results depending on the population studied. Most studies have focused on recurrent dislocation as the primary outcome of interest, whereas metrics such as subluxation, return to athletics, and patient-reported outcome scores are also available in limited studies. The known risk factors for recurrence include young age and male gender. Surgical stabilization should be considered in populations at higher risk of recurrence and has been shown in Level I studies to result in reduction of recurrence and improvement in patient-reported outcomes...
December 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
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