Zahid Hussain, Bhudev C Das, Syed A Husain, Sunil K Polipalli, Tanzeel Ahmed, Nargis Begum, Subhash Medhi, Alice Verghese, Mohammad Raish, Apiradee Theamboonlers, Yong Poovorawan, Premashis Kar
AIM: To undertake analysis of hepatitis A viral load, alanine aminotransferase (ALT), and viral genotypes with duration of viremia, and to correlate these parameters with CD4(+)/ CD8(+) lymphocyte populations that control cell-mediated immunity. METHODS: Cell counts were carried out using fresh whole blood collected in EDTA vials using a fluorescence activated cell sorter. Hepatitis A virus (HAV) RNA was extracted from blood serum, reverse transcribed into cDNA and quantified by Real-Time polymerase chain reaction and was genotyped...
August 7, 2006: World Journal of Gastroenterology: WJG