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https://www.readbyqxmd.com/read/28881949/heat-tolerance-induction-of-the-indian-meal-moth-lepidoptera-pyralidae-is-accompanied-by-upregulation-of-heat-shock-proteins-and-polyols
#1
Minhyun Kim, Seunghee Lee, Yong Shik Chun, Jahyun Na, Hyeok Kwon, Wook Kim, Yonggyun Kim
The Indian meal moth, Plodia interpunctella, causes massive damage to stored grains and processed foods. Heat treatment has been widely used to control insect pests infesting stored grains. However, heat treatment may result in unsatisfactory control owing to heat tolerance of target insects. This study quantified the heat tolerance and analyzed its induction in P. interpunctella. Susceptibility of P. interpunctella to different high temperatures was assessed in all developmental stages. Heat treatment at 44 °C for 1 h caused significant mortalities to all developmental stages, with late-instar larvae exhibiting the highest tolerance...
August 1, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28855547/tylophorine-analogs-allosterically-regulates-heat-shock-cognate-protein-70-and-inhibits-hepatitis-c-virus-replication
#2
Ying Wang, Sangwon Lee, Ya Ha, Wing Lam, Shao-Ru Chen, Ginger E Dutschman, Elizabeth A Gullen, Susan P Grill, Yao Cheng, Alois Fürstner, Samson Francis, David C Baker, Xiaoming Yang, Kuo-Hsiung Lee, Yung-Chi Cheng
Tylophorine analogs have been shown to exhibit diverse activities against cancer, inflammation, arthritis, and lupus in vivo. In this study, we demonstrated that two tylophorine analogs, DCB-3503 and rac-cryptopleurine, exhibit potent inhibitory activity against hepatitis C virus (HCV) replication in genotype 1b Con 1 isolate. The inhibition of HCV replication is at least partially mediated through cellular heat shock cognate protein 70 (Hsc70). Hsc70 associates with the HCV replication complex by primarily binding to the poly U/UC motifs in HCV RNA...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28855508/chaperones-rescue-the-energetic-landscape-of-mutant-cftr-at-single-molecule-and-in-cell
#3
Miklos Bagdany, Guido Veit, Ryosuke Fukuda, Radu G Avramescu, Tsukasa Okiyoneda, Imad Baaklini, Jay Singh, Guy Sovak, Haijin Xu, Pirjo M Apaja, Sara Sattin, Lenore K Beitel, Ariel Roldan, Giorgio Colombo, William Balch, Jason C Young, Gergely L Lukacs
Molecular chaperones are pivotal in folding and degradation of the cellular proteome but their impact on the conformational dynamics of near-native membrane proteins with disease relevance remains unknown. Here we report the effect of chaperone activity on the functional conformation of the temperature-sensitive mutant cystic fibrosis channel (∆F508-CFTR) at the plasma membrane and after reconstitution into phospholipid bilayer. Thermally induced unfolding at 37 °C and concomitant functional inactivation of ∆F508-CFTR are partially suppressed by constitutive activity of Hsc70 and Hsp90 chaperone/co-chaperone at the plasma membrane and post-endoplasmic reticulum compartments in vivo, and at single-molecule level in vitro, indicated by kinetic and thermodynamic remodeling of the mutant gating energetics toward its wild-type counterpart...
August 30, 2017: Nature Communications
https://www.readbyqxmd.com/read/28851515/alterations-in-the-e3-ligases-parkin-and-chip-result-in-unique-metabolic-signaling-defects-and-mitochondrial-quality-control-issues
#4
REVIEW
Britney N Lizama, Amy M Palubinsky, BethAnn McLaughlin
E3 ligases are essential scaffold proteins, facilitating the transfer of ubiquitin from E2 enzymes to lysine residues of client proteins via isopeptide bonds. The specificity of substrate binding and the expression and localization of E3 ligases can, however, endow these proteins with unique features with variable effects on mitochondrial, metabolic and CNS function. By comparing and contrasting two E3 ligases, Parkin and C-terminus of HSC70-Interacting protein (CHIP) we seek to highlight the biophysical properties that may promote mitochondrial dysfunction, acute stress signaling and critical developmental periods to cease in response to mutations in these genes...
August 26, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28801948/a-proteomics-method-using-immunoaffinity-fluorogenic-derivatization-liquid-chromatography-tandem-mass-spectrometry-fd-lc-ms-ms-to-identify-a-set-of-interacting-proteins
#5
Katsunori Nakata, Ryoichi Saitoh, Masaki Ishigai, Kazuhiro Imai
Biological functions in organisms are usually controlled by a set of interacting proteins, and identifying the proteins that interact is useful for understanding the mechanism of the functions. Immunoprecipitation is a method that utilizes the affinity of an antibody to isolate and identify the proteins that have interacted in a biological sample. In this study, the FD-LC-MS/MS method, which involves fluorogenic derivatization followed by separation and quantification by HPLC and finally identification of proteins by HPLC-tandem mass spectrometry, was used to identify proteins in immunoprecipitated samples, using Heat shock protein 90 (HSP90) as a model of an interacting protein in HepaRG cells...
August 12, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28757353/osmotic-and-heat-stress-dependent-regulation-of-mlk4%C3%AE-and-mlk3-by-the-chip-e3-ligase-in-ovarian-cancer-cells
#6
Natalya A Blessing, Srimathi Kasturirangan, Evan M Zink, April L Schroyer, Deborah N Chadee
Mixed Lineage Kinase 3 (MLK3), a member of the MLK subfamily of protein kinases, is a mitogen-activated protein (MAP) kinase kinase kinase (MAP3K) that activates MAPK signalling pathways and regulates cellular responses such as proliferation, invasion and apoptosis. MLK4β, another member of the MLK subfamily, is less extensively studied, and the regulation of MLK4β by stress stimuli is not known. In this study, the regulation of MLK4β and MLK3 by osmotic stress, thermostress and heat shock protein 90 (Hsp90) inhibition was investigated in ovarian cancer cells...
November 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28741080/transcriptomic-analyses-reveal-clathrin-mediated-endocytosis-involved-in-symbiotic-seed-germination-of-gastrodia-elata
#7
Xu Zeng, Yuanyuan Li, Hong Ling, Sisi Liu, Mengmeng Liu, Juan Chen, Shunxing Guo
BACKGROUND: Gastrodia elata is a well-known medicinal orchid. In nature, the germination rate of G. elata is extremely poor, because there is no endosperm within the mature seed. It is crucial for G. elata to obtain nutrition from mycorrhizal fungi (Mycena) at the early-stage of germination. After germination, the seed gives rise to a protocorm. However, there are no "omic" studies on understanding the interaction between Gastrodia and Mycena. Here, we used transcriptomic approaches to explore changes in seed germination of G...
December 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28731191/carboxy-terminus-hsc70-interacting-protein-exerts-a-tumor-inhibition-function-in-head-and-neck-cancer
#8
Meng Xiao, Ming Yan, Jianjun Zhang, Qin Xu, Wantao Chen
Several independent studies have reported the roles of the E3 ubiquitin ligase, carboxy-terminus Hsc70 interacting protein (CHIP) in various types of cancers. However, the biological effects of CHIP vary in regards to different cancers, and the role of CHIP in head and neck cancers (HNCs) remains unknown. In the present study, CHIP overexpression plasmids and CHIP knockdown lentivirus were constructed to affect the expression levels of CHIP protein and biological behaviors in HNC cell lines bilaterally. The biological behaviors regulated by CHIP in HNCs were investigated both in vivo and in vitro with a series of assays and analyses...
July 17, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28719655/protein-analysis-and-gene-expression-indicate-differential-vulnerability-of-iberian-fish-species-under-a-climate-change-scenario
#9
Tiago F Jesus, João M Moreno, Tiago Repolho, Alekos Athanasiadis, Rui Rosa, Vera M F Almeida-Val, Maria M Coelho
Current knowledge on the biological responses of freshwater fish under projected scenarios of climate change remains limited. Here, we examine differences in the protein configuration of two endemic Iberian freshwater fish species, Squalius carolitertii and the critically endangered S. torgalensis that inhabit in the Atlantic-type northern and in the Mediterranean-type southwestern regions, respectively. We performed protein structure modeling of fourteen genes linked to protein folding, energy metabolism, circadian rhythms and immune responses...
2017: PloS One
https://www.readbyqxmd.com/read/28702780/the-purine-rich-element-binding-protein-chpur-%C3%AE-negatively-regulates-hsc70-transcription-in-crassostrea-hongkongensis
#10
Delin Xu, Di Wang, Miao Cui, Qizhong Zhang
ChPur-α, a purine-rich element-binding protein, was discovered showing affinity to the ChHsc70 promoter in Crassostrea hongkongensis by DNA affinity purification and mass spectrometry analysis. Direct interaction between purified ChPur-α and the ChHsc70 promoter region was demonstrated by electrophoretic mobility shift assay in vitro. ChPur-α reduction led to clear enhancements of ChHsc70 transcription in the hemocytes of C. hongkongensis. Consistently, ChPur-α overexpression in heterologous HEK293T cells correlated with repressive phenotype in ChHsc70 promoter expression...
July 13, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28676984/the-regulation-of-heat-shock-proteins-in-response-to-dehydration-in-xenopus-laevis
#11
Bryan E Luu, Sanoji Wijenayake, Amal I Malik, Kenneth B Storey
African clawed frogs (Xenopus laevis) endure bouts of severe drought in their natural habitats and survive the loss of approximately 30% of total body water due to dehydration. To investigate molecular mechanisms employed by X. laevis during periods of dehydration, the heat shock protein response, a vital component of the cytoprotective stress response, was characterized. Using western immunoblotting and multiplex technology, the protein levels of HSP27, HSP40, HSP60, HSP70, HSC70, and HSP90 were quantified in the liver, skeletal muscle, kidney, lung, and testes from control frogs and those that underwent medium or high dehydration (~16 or ~30% loss of total body water)...
July 5, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28640320/molecular-signature-of-coronary-stent-thrombosis-oxidative-stress-and-innate-immunity-cells
#12
Judit Cubedo, Ana Blasco, Teresa Padro, Ilaria Ramaiola, Oriol Juan-Babot, Javier Goicolea, José Antonio Fernández-Díaz, Juan Francisco Oteo, Lina Badimon
The clinical impact of in-stent thrombosis is high because it is associated with high mortality and 20 % of the patients suffer a recurrent event within the two following years. The aim of this study was to characterise the morphologic and proteomic profile of in-stent thrombi (IST) in comparison to thrombi developed on native coronary arteries (CT) to identify a differential molecular signature. The study included 45 patients with ST-elevation-myocardial-infarction (STEMI) treated by primary-percutaneous-intervention and thrombus aspiration: 21 had IST and 24 had CT...
August 30, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28634860/molecular-chaperone-hsp70-and-its-constitutively-active-form-hsc70-play-an-indispensable-role-during-eye-development-of-drosophila-melanogaster
#13
Ajay Kumar, Anand K Tiwari
In the present study, we demonstrate that molecular chaperone Hsp70 and Hsc70 is essential for normal organization and development of ommatidial cells in Drosophila melanogaster eye. An exogenously expressed dominant negative mutant of Hsp70 (K71E) and Hsc70.4 (K71S and D206S) in an eye-specific manner resulted in eye degeneration that includes loss of eye pigment, disorganized ommatidia, abnormality in bristle cell arrangement and reduction in the eye size. The developmental organization of ommatidial cells (cone, photoreceptor, pigment, and bristle cell complex) was disturbed in Hsp70 and Hsc70 mutants...
June 20, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28615625/anti-leukemia-activity-of-a-hsp70-inhibitor-and-its-hybrid-molecules
#14
Seong-Hyun Park, Won-Je Kim, Hui Li, Wonil Seo, Sang-Hyun Park, Hwan Kim, Sang Chul Shin, Erik R P Zuiderweg, Eunice EunKyeong Kim, Taebo Sim, Nak-Kyoon Kim, Injae Shin
In this study we examined the anti-leukemia activity of a small molecule inhibitor of Hsp70 proteins, apoptozole (Az), and hybrids in which it is linked to an inhibitor of either Hsp90 (geldanamycin) or Abl kinase (imatinib). The results of NMR studies revealed that Az associates with an ATPase domain of Hsc70 and thus blocks ATP binding to the protein. Observations made in the cell study indicated that Az treatment promotes leukemia cell death by activating caspase-dependent apoptosis without affecting the caspase-independent apoptotic pathway...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28603283/txndc5-synergizes-with-hsc70-to-exacerbate-the-inflammatory-phenotype-of-synovial-fibroblasts-in-rheumatoid-arthritis-through-nf-%C3%AE%C2%BAb-signaling
#15
Lin Wang, Hongyan Dong, Guanhua Song, Rui Zhang, Jihong Pan, Jinxiang Han
The upregulated expression of thioredoxin domain-containing protein 5 (TXNDC5) is associated with rheumatoid arthritis in patients and model mice. However, the underlying mechanism by which TXNDC5 influences the pathological activation of rheumatoid arthritis synovial fibroblasts (RASFs) remains unknown. In this study, we show that TXNDC5 expression in RASFs and their cytokine production are significantly upregulated in response to LPS, TNF-α and IL-6, but suppressed by transfection with TXNDC5-siRNA. TXNDC5 is further validated as the direct target of NF-κB signaling...
June 12, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28579939/the-role-of-co-chaperones-in-synaptic-proteostasis-and-neurodegenerative-disease
#16
REVIEW
Erica L Gorenberg, Sreeganga S Chandra
Synapses must be preserved throughout an organism's lifespan to allow for normal brain function and behavior. Synapse maintenance is challenging given the long distances between the termini and the cell body, reliance on axonal transport for delivery of newly synthesized presynaptic proteins, and high rates of synaptic vesicle exo- and endocytosis. Hence, synapses rely on efficient proteostasis mechanisms to preserve their structure and function. To this end, the synaptic compartment has specific chaperones to support its functions...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28559423/hsc70-chaperone-activity-is-required-for-the-cytosolic-slow-axonal-transport-of-synapsin
#17
Archan Ganguly, Xuemei Han, Utpal Das, Lina Wang, Jonathan Loi, Jichao Sun, Daniel Gitler, Ghislaine Caillol, Christophe Leterrier, John R Yates, Subhojit Roy
Soluble cytosolic proteins vital to axonal and presynaptic function are synthesized in the neuronal soma and conveyed via slow axonal transport. Our previous studies suggest that the overall slow transport of synapsin is mediated by dynamic assembly/disassembly of cargo complexes followed by short-range vectorial transit (the "dynamic recruitment" model). However, neither the composition of these complexes nor the mechanistic basis for the dynamic behavior is understood. In this study, we first examined putative cargo complexes associated with synapsin using coimmunoprecipitation and multidimensional protein identification technology mass spectrometry (MS)...
July 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28510570/bag-1-as-a-biomarker-in-early-breast-cancer-prognosis-a-systematic-review-with-meta-analyses
#18
REVIEW
E S Papadakis, T Reeves, N H Robson, T Maishman, G Packham, R I Cutress
BACKGROUND: The co-chaperone protein Bcl-2-associated athanogene-1 (BAG-1) is overexpressed in breast cancer and has been incorporated in the oncotype DX and PAM50 breast cancer prognostic assays. Bcl-2-associated athanogene-1 exists as multiple protein isoforms that interact with diverse partners, including chaperones Hsc70/Hsp70, Ser/Thr kinase Raf-1 and Bcl-2, to promote cancer cell survival. The BAG-1L isoform specifically binds to and increases the transcriptional activity of oestrogen receptor in cells, and in some, but not all studies, BAG-1 expression is predictive of clinical outcome in breast cancer...
June 6, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28509353/engineering-heat-tolerance-in-potato-by-temperature-dependent-expression-of-a-specific-allele-of-heat-shock-cognate-70
#19
Almudena Trapero-Mozos, Wayne L Morris, Laurence J M Ducreux, Karen McLean, Jennifer Stephens, Lesley Torrance, Glenn J Bryan, Robert D Hancock, Mark A Taylor
For many commercial potato cultivars, tuber yield is optimal at average day time temperatures in the range of 14-22⁰C. Further rises in ambient temperature can reduce or completely inhibit potato tuber production, with damaging consequences for both producer and consumer. The aim of this study was to use a genetic screen based on a model tuberisation assay to identify Quantitative Trait Loci (QTL) associated with enhanced tuber yield. A candidate gene encoding HSc70 was identified within one of the three QTL intervals associated with elevated yield in a Phureja-Tuberosum hybrid diploid potato population (06H1)...
May 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28484369/differential-targeting-of-hsp70-heat-shock-proteins-hspa6-and-hspa1a-with-components-of-a-protein-disaggregation-refolding-machine-in-differentiated-human-neuronal-cells-following-thermal-stress
#20
Catherine A S Deane, Ian R Brown
Heat shock proteins (Hsps) co-operate in multi-protein machines that counter protein misfolding and aggregation and involve DNAJ (Hsp40), HSPA (Hsp70), and HSPH (Hsp105α). The HSPA family is a multigene family composed of inducible and constitutively expressed members. Inducible HSPA6 (Hsp70B') is found in the human genome but not in the genomes of mouse and rat. To advance knowledge of this little studied HSPA member, the targeting of HSPA6 to stress-sensitive neuronal sites with components of a disaggregation/refolding machine was investigated following thermal stress...
2017: Frontiers in Neuroscience
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