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membrane protein folding

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https://www.readbyqxmd.com/read/29773177/participation-of-selenoproteins-localized-in-the-er-in-the-processes-occurring-in-this-organelle-and-in-the-regulation-of-carcinogenesis-associated-processes
#1
REVIEW
Elena Gennadyevna Varlamova
The functions performed by the ER are diverse: synthesis of steroid hormones, synthesis of proteins for the plasma membrane, lysosomes, as well as proteins meant for exocytosis, protein folding, formation of disulfide bonds, N-linked glycosylation, etc. Selenoproteins localized in this organelle are definitely involved in the processes occurring in it, and the most common of them include participation in protein degradation, regulation of ER stress and redox metabolism. ER stress has been registered in many types of cancer cells...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29766714/the-outer-membrane-vesicle-proteome-of-porphyromonas-gingivalis-is-differentially-modulated-relative-to-the-outer-membrane-in-response-to-heme-availability
#2
Paul D Veith, Caroline Luong, Keng H Tan, Stuart G Dashper, Eric C Reynolds
Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification...
May 16, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29764287/function-of-a-membrane-embedded-domain-evolutionarily-multiplied-in-the-gpi-lipid-anchor-pathway-proteins-pig-b-pig-m-pig-u-pig-w-pig-v-and-pig-z
#3
Birgit Eisenhaber, Swati Sinha, Wing-Cheong Wong, Frank Eisenhaber
Distant homology relationships among proteins with many transmembrane regions (TMs) are difficult to detect as they are clouded by the TMs' hydrophobic compositional bias and mutational divergence in connecting loops. In the case of several GPI lipid anchor biosynthesis pathway components, the hidden evolutionary signal can be revealed with dissectHMMER, a sequence similarity search tool focusing on fold-critical, high complexity sequence segments. We find that a sequence module with 10 TMs in PIG-W, described as acyl transferase, is homologous to PIG-U, a transamidase subunit without characterized molecular function, and to mannosyltransferases PIG-B, PIG-M, PIG-V and PIG-Z...
May 15, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29764003/a-bioartificial-kidney-device-with-polarized-secretion-of-immune-modulators
#4
N V Chevtchik, M Mihajlovic, M Fedecostante, L Bolhuis-Versteeg, J Sastre Toraño, R Masereeuw, D Stamatialis
The accumulation of protein-bound toxins in dialyzed patients is strongly associated with their high morbidity and mortality. The bioartificial kidney device (BAK), containing proximal tubule epithelial cells (PTEC) seeded on functionalized synthetic hollow fiber membranes (HFM), may be a powerful solution for the active removal of those metabolites. In an earlier study, we developed an upscaled BAK containing conditionally immortalized human PTEC (ciPTEC) with functional organic cationic transporter 2 (OCT2)...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29763546/direct-structural-annotation-of-membrane-protein-aggregation-loci-using-peptide-based-reverse-mapping
#5
Muralikrishna Lella, Radhakrishnan Mahalakshmi
Membrane protein aggregation is associated with neurodegenerative diseases. Despite remarkable advances to map protein aggregation, molecular elements that drive the structural transition from functional to amyloidogenic β-sheet polymers remain elusive. Here, we report a simple and reliable reverse-mapping method to identify the molecular elements. We validate our approach by obtaining molecular details of aggregation loci of human β-barrel nanopore ion channels that are vital for cell survival. By coupling bottom-up synthesis with time-resolved aggregation kinetics and high-resolution imaging, we identify molecular elements that switch folded channels to polymeric β-rich aggregates...
May 15, 2018: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29760092/noncatalytic-chalcone-isomerase-fold-proteins-in-humulus-lupulus-are-auxiliary-components-in-prenylated-flavonoid-biosynthesis
#6
Zhaonan Ban, Hao Qin, Andrew J Mitchell, Baoxiu Liu, Fengxia Zhang, Jing-Ke Weng, Richard A Dixon, Guodong Wang
Xanthohumol (XN) and demethylxanthohumol (DMX) are specialized prenylated chalconoids with multiple pharmaceutical applications that accumulate to high levels in the glandular trichomes of hops ( Humulus lupulus L.). Although all structural enzymes in the XN pathway have been functionally identified, biochemical mechanisms underlying highly efficient production of XN have not been fully resolved. In this study, we characterized two noncatalytic chalcone isomerase (CHI)-like proteins (designated as HlCHIL1 and HlCHIL2) using engineered yeast harboring all genes required for DMX production...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29755357/-n-glycosylation-of-lipocalin-2-is-not-required-for-secretion-or-exosome-targeting
#7
Erawan Borkham-Kamphorst, Eddy Van de Leur, Steffen K Meurer, Eva M Buhl, Ralf Weiskirchen
Lipocalin 2 (LCN2) is a highly conserved secreted adipokine acting as a serum transport protein for small hydrophobic molecules such as fatty acids and steroids. In addition, LCN2 limits bacterial growth by sequestering iron-containing siderophores and further protects against intestinal inflammation and tumorigenesis associated with alterations in the microbiota. Human LCN2 contains one N -glycosylation site conserved in other species. It was postulated that this post-translational modification could facilitate protein folding, protects from proteolysis, is required for proper trafficking from the Golgi apparatus to the cell surface, and might be relevant for effective secretion...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29753087/effects-of-salazosulfapyridine-on-the-profile-of-cell-surface-proteins-revealed-by-biotinylation-of-cell-surface-proteins-and-2-dimentional-electrophoresis
#8
Kazuki Omoteyama, Toshiyuki Sato, Mitsumi Arito, Masaaki Sato, Naoya Suematsu, Manae S Kurokawa, Tomohiro Kato
OBJECTIVE: We investigated effects of salazosulfapyridine (SASP) on the protein profile of cell surface (CS)-proteins of SW982, a human synovial sarcoma cell line, using biotinylation of CS-proteins and 2-dimensional fluorescence difference gel electrophoresis (2D-DIGE). METHODS: SW982 cells were treated with SASP and its metabolites, sulfapyridine (SP) and 5-aminosalicylic acid (5ASA). Then the cells were treated with a membrane-impermeable biotinylating reagent...
May 9, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29752412/properly-folded-and-functional-porb-from-neisseria-gonorrhoeae-inhibits-dendritic-cell-stimulation-of-cd4-t-cell-proliferation
#9
Weiyan Zhu, Joshua Tomberg, Kayla J Knilans, James E Anderson, Karen P McKinnon, Gregory D Sempowski, Robert A Nicholas, Joseph A Duncan
Neisseria gonorrhoeae is an exclusive human pathogen that evades the host immune system through multiple mechanisms. We have shown that N. gonorrhoeae  suppresses the capacity of antigen-presenting cells to induce CD4+ T cell proliferation. In this study, we sought to determine the gonococcal factors involved in this adaptive immune suppression. We show that suppression of the capacity of antigen-pulsed dendritic cells to induce T cell proliferation is recapitulated by administration of a high molecular weight fraction of conditioned medium from N...
May 11, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29752257/multidrug-resistance-protein-1-mrp1-abcc1-mediated-cellular-protection-and-transport-of-methylated-arsenic-metabolites-differs-between-human-cell-lines
#10
Mayukh Banerjee, Gurnit Kaur, Brayden D Whitlock, Michael W Carew, X Chris Le, Elaine M Leslie
The ATP-binding cassette (ABC) transporter Multidrug Resistance Protein 1 (MRP1/ABCC1) is known to protect cells from the proven human carcinogen arsenic through the cellular efflux of arsenic triglutathione [As(GS)3], and the diglutathione conjugate of the highly toxic monomethylarsonous acid (MMAIII) [MMA(GS)2]. Previously, differences in MRP1 phosphorylation (at Y920/S921) and N-glycosylation (at N19/N23) were associated with marked differences in As(GS)3 transport kinetics between HEK293 and HeLa cell lines...
May 11, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29750191/structures-of-monomeric-and-oligomeric-forms-of-the-toxoplasma-gondii-perforin-like-protein-1
#11
Tao Ni, Sophie I Williams, Saša Rezelj, Gregor Anderluh, Karl Harlos, Phillip J Stansfeld, Robert J C Gilbert
Toxoplasma and Plasmodium are the parasitic agents of toxoplasmosis and malaria, respectively, and use perforin-like proteins (PLPs) to invade host organisms and complete their life cycles. The Toxoplasma gondii PLP1 ( Tg PLP1) is required for efficient exit from parasitophorous vacuoles in which proliferation occurs. We report structures of the membrane attack complex/perforin (MACPF) and Apicomplexan PLP C-terminal β-pleated sheet (APCβ) domains of Tg PLP1. The MACPF domain forms hexameric assemblies, with ring and helix geometries, and the APCβ domain has a novel β-prism fold joined to the MACPF domain by a short linker...
March 2018: Science Advances
https://www.readbyqxmd.com/read/29749501/placental-protein-14-as-a-potential-biomarker-for-diagnosis-of-preterm-premature-rupture-of-membranes
#12
Yanyun Wang, Haibo Luo, Guanglu Che, Yanqin Li, Jun Gao, Qiongli Yang, Bin Zhou, Linbo Gao, Tao Wang, Yujie Liang, Lin Zhang
Premature rupture of membranes (PROM) is a common pregnancy complication that frequently results in maternal and perinatal morbidity. The present methods for diagnosing PROM do not satisfy clinical requirements. The present study aimed to examine the proteome profile of amniotic fluid (AF) and maternal plasma, screen unique proteins in AF, and evaluate their diagnostic value for diagnosing PROM. The proteome profiles of AF and maternal plasma were examined via liquid chromatography coupled with tandem mass spectrometry‑based proteomic techniques...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29744892/-epigallocatechin-3-gallate-induces-grp78-accumulation-in-the-er-and-shifts-mesothelioma-constitutive-upr-into-proapoptotic-er-stress
#13
Simona Martinotti, Elia Ranzato, Bruno Burlando
GRP78 is a molecular chaperone of the endoplasmic reticulum (ER) that aids proper folding of nascent polypeptides. When unfolded proteins accumulate, GRP78 triggers unfolded protein response (UPR), involving activation of transcription factors like XBP1 and CHOP that may restore cell homeostasis. Increased expression of GRP78 and mild UPR can be constitutive in cancer cells, hindering apoptosis, and promoting cell survival, for example, by GRP78 relocation to the plasma membrane that activates MAPK and PI3 K/AKT pathways...
May 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29742582/changes-in-microrna-expression-level-of-circulating-platelets-contribute-to-platelet-defect-after-cardiopulmonary-bypass
#14
Nobuhiro Mukai, Yoshinobu Nakayama, Sachiyo Ishi, Satoru Ogawa, Sachiko Maeda, Natuki Anada, Satoshi Murakami, Toshiki Mizobe, Teiji Sawa, Yasufumi Nakajima
OBJECTIVES: Platelet defect mechanisms after cardiopulmonary bypass remain unclear. Our hypothesis microRNA expressions in circulating platelets significantly change between pre and post cardiopulmonary bypass, and consequent messenger RNA and protein expression level alterations cause postcardiopulmonary bypass platelet defect. DESIGN: Single-center prospective observational study. SETTING: Operating room of Kyoto Prefectural University of Medicine...
May 8, 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/29742327/membrane-proteome-of-invasive-retinoblastoma-differential-proteins-and-biomarkers
#15
Ravikanth Danda, Kalaivani Ganapathy, Gajanan Sathe, Anil K Madugundu, Uma Maheswari Krishnan, Vikas Khetan, Pukhraj Rishi, Harsha Gowda, Akhilesh Pandey, Krishnakumar Subramanian, Ts Keshava Prasad, Sailaja V Elchuri
PURPOSE: Retinoblastoma (RB) is a pediatric ocular cancer which is caused due to the aberrations in the RB1 gene. The changes in the membrane proteomics would help in understanding the development of the retinoblastoma and could identify candidates for biomarkers and therapy. EXPERIMENTAL DESIGN: We performed quantitative proteomics on the enriched membrane fractions from pooled normal retina (n = 5) and pooled retinoblastoma tissues (n = 5). The proteins were tryptic digested and tagged with iTRAQ labels...
May 9, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29740434/lack-of-evidence-for-a-direct-interaction-of-progranulin-and-tumor-necrosis-factor-receptor-1-and-tumor-necrosis-factor-receptor-2-from-cellular-binding-studies
#16
Isabell Lang, Simone Füllsack, Harald Wajant
Progranulin (PGRN) is a secreted anti-inflammatory protein which can be processed by neutrophil proteases to various granulins. It has been reported that at least a significant portion of the anti-inflammatory effects of PGRN is due to direct high affinity binding to tumor necrosis factor receptor-1 (TNFR1) and TNFR2 and inhibition of tumor necrosis factor (TNF)-induced TNFR1/2 signaling. Two studies failed to reproduce the interaction of TNFR1 and TNFR2 with PGRN, but follow up reports speculated that this was due to varying experimental circumstances and/or the use of PGRN from different sources...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29740410/growth-and-extended-survival-of-escherichia-coli-o157-h7-in-soil-organic-matter
#17
Gitanjali NandaKafle, Amy A Christie, Sébastien Vilain, Volker S Brözel
Enterohaemorrhagic Escherichia coli , such as serotype O157:H7, are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection. E. coli is able to grow in the tissue of food plants such as spinach. While fecal contamination is the primary suspect, soil has been underestimated as a potential reservoir. Persistence of bacterial populations in open systems is the product of growth, death, predation, and competition...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29740124/a-targeted-boost-and-sort-immunization-strategy-using-escherichia-coli-bama-identifies-rare-growth-inhibitory-antibodies
#18
Rajesh Vij, Zhonghua Lin, Nancy Chiang, Jean-Michel Vernes, Kelly M Storek, Summer Park, Joyce Chan, Y Gloria Meng, Laetitia Comps-Agrar, Peng Luan, Sophia Lee, Kellen Schneider, Jack Bevers, Inna Zilberleyb, Christine Tam, Christopher M Koth, Min Xu, Avinash Gill, Marcy R Auerbach, Peter A Smith, Steven T Rutherford, Gerald Nakamura, Dhaya Seshasayee, Jian Payandeh, James T Koerber
Outer membrane proteins (OMPs) in Gram-negative bacteria are essential for a number of cellular functions including nutrient transport and drug efflux. Escherichia coli BamA is an essential component of the OMP β-barrel assembly machinery and a potential novel antibacterial target that has been proposed to undergo large (~15 Å) conformational changes. Here, we explored methods to isolate anti-BamA monoclonal antibodies (mAbs) that might alter the function of this OMP and ultimately lead to bacterial growth inhibition...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29739115/upregulation-of-breast-cancer-resistance-protein-expression-was-decreased-in-plasma-membrane-of-colon-cancer-with-metastasis-of-lymphatic-node
#19
Ao Wu, Jiaoli Zhang, Liyan Zeng, Feng Li, Zhaoguang Dong, Yanling Feng, Xiaofang Jia, Jun-O Jin, Lijun Zhang
BACKGROUND: ATP-binding cassette sub-family G member 2 (ABCG2), an ABC transport protein involved in the efflux of anticancer compounds, has been reported to have altered expression levels in several cancers including breast, colon, and gastric cancer, etc. However, its expression change (up- or down-regulated in cancer) is still contradictory. METHODS: We performed immunohistochemistry to examine ABCG2 expression in the microarray with 90 pairs of colon cancer and their adjacent normal tissues...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29739064/serum-proteomic-analysis-by-tandem-mass-tags-tmt-based-quantitative-proteomics-in-gastric-cancer-patients
#20
Aidan Huang, Meiyu Zhang, Taijie Li, Xue Qin
BACKGROUND: To use the tandem mass tags (TMT) based quantitative proteomics technique and bioinformatics method to identify potential serum diagnostic biomarkers for gastric cancer (GC). METHODS: This study enrolled GC patients and healthy control subjects. Mixed serum samples were pooled with 10 individual samples. The high-abundance proteins depleted serum proteins were collected by removing abundance albumin and immunoglobulin G (IgG). After desalting and ultrafiltration, the trypsin digested proteins were analyzed using TMT based quantitative proteomics system...
May 1, 2018: Clinical Laboratory
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