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Shun Tamaki, Mitsuhiko Yagi, Yuki Nishihata, Hideki Yamaji, Yasushi Shigeri, Tomohide Uno, Hiromasa Imaishi
The aromatic compound p -hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals and CYP53A15 from fungus Cochliobolus lunatus is capable of the hydroxylation from benzoate to PHBA. Here we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells co-expressing CYP53A15 and human NADPH-P450 oxidoreductase ( CPR ) genes as a redox partner...
March 15, 2018: Journal of Microbiology and Biotechnology
Masaru Nakagawa, Sigeyuki Uno, Noriyoshi Iriyama, Manabu Matsunawa, Makoto Makishima, Jin Takeuchi, Isao Tsuboi, Yoshihiro Hatta, Masami Takei
Benzo[a]pyrene (BaP) is an environmental pollutant found in cigarette smoke and is implicated as a causative agent of tobacco-related diseases, such as arteriosclerosis. In contrast, vitamin D signaling, which is principally mediated by conversion of vitamin D to the active form, 1α,25-dihydroxyvitamin D3 [1,25(OH)2 D3 ], decreases cardiovascular disease risk. However, combined treatment with BaP and 1,25(OH)2 D3 enhances BaP toxicity, including BaP-DNA adduct formation. We further investigated the cross-talk between BaP and 1,25(OH)2 D3 signaling pathways, and found that combined treatment with these compounds induces mRNA and protein expression of plasminogen activator inhibitor 1 (PAI-1) in monocyte/macrophage-derived THP-1 and U937 cells...
March 7, 2018: Toxicology and Applied Pharmacology
S Perez-Protto, R Nazemian, M Matta, P Patel, K J Wagner, S Q Latifi, D J Lebovitz, J D Reynolds
Many deceased by neurologic criteria donors are administered inhalational agents during organ recovery surgery-a process that is characterised by warm and cold ischaemia followed by warm reperfusion. In certain settings, volatile anaesthetics (VA) are known to precondition organs to protect them from subsequent ischaemia-reperfusion injury. As such, we hypothesised that exposure to VA during organ procurement would improve post-graft survival. Lifebanc (organ procurement organisation [OPO] for NE Ohio) provided the investigators with a list of death by neurologic criteria organ donors cared for at three large tertiary hospitals in Cleveland between 2006 and 2016-details about the surgical recovery phase were extracted from the organ donors' medical records...
March 2018: Anaesthesia and Intensive Care
Mikael Frisk, Annie Jonsson, Stefan Sellman, Patrik Flisberg, Mikael Rönnqvist, Uno Wennergren
Each year, more than three million animals are transported from farms to abattoirs in Sweden. Animal transport is related to economic and environmental costs and a negative impact on animal welfare. Time and the number of pick-up stops between farms and abattoirs are two key parameters for animal welfare. Both are highly dependent on efficient and qualitative transportation planning, which may be difficult if done manually. We have examined the benefits of using route optimization in cattle transportation planning...
2018: PloS One
Koichiro Mandai, Koji Uno, Yuki Ueda, Yusuke Okada, Kenjiro Yasuda
Background: Efficacy of a physician-controlled wire-guided cannulation of the bile duct has been reported. This study aimed to evaluate a novel sphincterotome with a short tip and a short wire, which can be bowed compactly compared to conventional sphincterotomes. Methods: This was a single-arm, single-center, prospective study. We enrolled patients with choledocholithiasis and/or obstructive jaundice and/or cholangitis for whom endoscopic sphincterotomy was planned...
February 2018: Gastroenterology Research
Koji Amano, Tatsuya Morita, Jiro Miyamoto, Teruaki Uno, Hirofumi Katayama, Ryohei Tatara
PURPOSE: Few studies have investigated the need for nutritional support in advanced cancer patients in palliative care settings. Therefore, we conducted a questionnaire to examine the relationship between the perception of need for nutritional support and cancer cachexia and the prevalence of specific needs, perceptions, and beliefs in nutritional support. METHODS: We conducted a questionnaire in palliative care settings. Patients were classified into two groups: (1) non-cachexia/pre-cachexia and (2) cachexia/refractory cachexia...
March 5, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
Ellen Mitchell, Kathleen M Loomes, Robert H Squires, David Goldberg
BACKGROUND: Recent data has suggested that pediatric patients waitlisted for a liver transplant frequently have liver offers declined. However, factors associated with liver offer decisions and center-level variability in practice patterns have not been explored. METHODS: We evaluated UNOS data on all match runs from 5/10/07-12/31/15 in which: 1) the liver was offered to ≥1 pediatric patient; 2) the transplant recipient was ranked in the first 40 positions for the organ offer; and 3) the donor was brain-dead and <50 years of age...
March 5, 2018: Liver Transplantation
Kazuhisa Honma, Satoshi Abe, Takeshi Endo, Narumi Uno, Mitsuo Oshimura, Tetsuya Ohbayashi, Yasuhiro Kazuki
Mouse artificial chromosome (MAC) vectors have several advantages as gene delivery vectors, such as stable and independent maintenance in host cells without integration, transferability from donor cells to recipient cells via microcell-mediated chromosome transfer (MMCT), and the potential for loading a megabase-sized DNA fragment. Previously, a MAC containing a multi-integrase platform (MI-MAC) was developed to facilitate the transfer of multiple genes into desired cells. Although the MI system can theoretically hold five gene-loading vectors (GLVs), there are a limited number of drugs available for the selection of multiple-GLV integration...
2018: PloS One
Kazuki Hirata, Taku Ogawa, Hiroyuki Fujikura, Yoshihiko Ogawa, Nobuyasu Hirai, Tomoko Nakagawa-Onishi, Kenji Uno, Masahiro Takeyama, Kei Kasahara, Fukumi Nakamura-Uchiyama, Mitsuru Konishi, Keiichi Mikasa
Few studies have analyzed the characteristics of patients who develop physical disorders after overseas travel. We retrospectively reviewed the medical records of 183 patients who visited Nara Medical University Hospital from 2008 to 2016 because of physical problems after traveling abroad. The main travel destinations were Southeast Asia (n = 100), Africa (n = 27), and South Asia (n = 23). The main reasons for the travel were leisure (n = 96), business (n = 51), and volunteer work (n = 19). The most common final diagnosis was gastrointestinal disease (n = 72), followed by febrile disease (n = 59) and respiratory disease (n = 19)...
March 1, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Asishana Osho, Michael Mulvihill, Nayan Lamba, Sameer Hirji, Babatunde Yerokun, Muath Bishawi, Philip Spencer, Nikhil Panda, Mauricio Villavicencio, Matthew Hartwig
BACKGROUND: Existing research demonstrates superior short term outcomes (Length of stay, 1 year survival) after lung transplantation in patients with preoperative functional independence. The aim of this study is to determine whether advantages remain significant in the long term. METHODS: The United Network for Organ Sharing (UNOS) database was queried for adult, first time, isolated lung transplantation records from 01/2005 to 12/2015. Stratification was performed based on Karnofsky Performance Status Score (3 groups) and on employment at the time of transplantation (2 groups)...
March 1, 2018: Annals of Thoracic Surgery
Shigeyuki Uno, Torahiko Tanaka, Hiroki Ashiba, Makoto Fujimaki, Mutsuo Tanaka, Yoshihiro Hatta, Masami Takei, Koichi Awazu, Makoto Makishima
Portable, on-site blood typing methods will help provide life-saving blood transfusions to patients during an emergency or natural calamity, such as significant earthquakes. We have previously developed waveguide-mode (WM) sensors for forward ABO and Rh(D) blood typing and detection of antibodies against hepatitis B virus and hepatitis C virus. In this study, we evaluated a WM-sensor for reverse ABO blood typing. Since reverse ABO blood typing is a method for detection of antibodies against type A and type B oligosaccharide antigens on the surface of red blood cells (RBCs), we fixed a synthetic type A or type B trisaccharide antigen on the sensor chip of the WM sensor...
February 27, 2018: Journal of Bioscience and Bioengineering
Kelly S DeMartini, Michael L Schilsky, Amanda Palmer, Dwain C Fehon, Paula Zimbrean, Stephanie S O'Malley, Hochang B Lee, Benjamin A Toll
BACKGROUND: Many liver transplantation programs require documented alcohol sobriety prior to United Network for Organ Sharing (UNOS) listing. This pilot study examined the feasibility of the first mobile, alcohol relapse prevention intervention for liver transplant patients with alcoholic liver disease (ALD). METHODS: This was a randomized 8-week pilot feasibility trial of a text message-based alcohol intervention. In-treatment assessment was conducted at 4 weeks (4W), and immediate posttreatment assessment was conducted at 8W...
March 2, 2018: Alcoholism, Clinical and Experimental Research
So Yamamoto, Shigeyasu Uno
This paper describes a paper-based biochemical sensor that realizes redox cycling with close interelectrode distance. Two electrodes, the generator and collector electrodes, can detect steady-state oxidation and reduction currents when suitable potential is held at each electrode. The sensor has two gold plates on both sides of a piece of chromatography paper and defines the interelectrode distance by the thickness of the paper (180 μm) without any micro-fabrication processes. Our proposed sensor geometry has successfully exhibited signatures of redox cycling...
February 28, 2018: Sensors
G Shekhtman, E Huang, G M Danovitch, P Martin, S Bunnapradist
In kidney-alone recipients, dual kidney transplantation (DKT) using "higher-risk" donor organs has shown outcomes comparable to those of single-kidney transplantation using expanded criteria donor (ECD) organs. To investigate the feasibility of a similar approach with combined kidney-liver transplantation, we identified 22 dual-kidney liver transplants (DKLT) and 3,044 single-kidney liver transplants (SKLT) performed in the United States between 2002 and 2012 using UNOS/OPTN registry data. We compared donor/recipient characteristics as well as graft/recipient survival between DKLT recipients and SKLT recipients of "higher-risk" kidneys [ECD and high kidney donor profile index (KDPI>85%) donors]...
March 1, 2018: Liver Transplantation
Nazarena Cataldo, Valeria Pegoraro, Claudio Ripellino, Valentino Bertasi, Roberta Di Turi, Maurizio Pastorello, Marco Gambera
Riassunto. Il presente studio intende confrontare il rischio di non persistenza e l'utilizzo delle risorse sanitarie nei pazienti con fibrillazione atriale non valvolare (FANV) naïve al trattamento con antagonisti della vitamina K (AVK) o con nuovi anticoagulanti orali (NAO). Partendo dai database amministrativi di cinque ASL italiane, sono stati selezionati e inclusi nello studio tutti i pazienti con almeno una dimissione ospedaliera per FANV dal 2011 al 2014. Tra questi, i nuovi utilizzatori di AVK o NAO nel 2014 sono stati identificati e seguiti a partire dalla data della prima prescrizione al verificarsi di uno qualsiasi dei seguenti eventi: interruzione della terapia superiore a 90 giorni, passaggio o aggiunta di una molecola differente rispetto a quella prescritta alla data indice, morte del paziente, fine del follow-up (dicembre 2015)...
February 2018: Recenti Progressi in Medicina
Yujiro Kinomura, Toshiki Tanaka, Takuma Aoyama, Masanori Hatasa, Shingo Minatoguchi, Masamitsu Iwasa, Yoshihisa Yamada, Takahide Nawa, Hiromitsu Kanamori, Masanori Kawasaki, Yukihiro Esaka, Bunji Uno, Kazuhiko Nishigaki, Shinya Minatoguchi
BACKGROUND: The role of endogenous adenosine in cardiac patients is still unclear, so we investigated the relationship between the plasma adenosine concentration and left ventricular (LV) function, LV dilation and LV wall thinning in cardiac patients.Methods and Results:In 97 cardiac patients, with angina pectoris, old myocardial infarction, dilated or hypertrophic cardiomyopathy, and valvular heart disease, plasma adenosine concentrations were measured using the LC-MS/MS system, and the LV function, LV end-diastolic dimension (LVDd), LV posterior wall thickness (LVPWth), and interventricular septum thickness (IVSth) were assessed by echocardiography...
February 28, 2018: Circulation Journal: Official Journal of the Japanese Circulation Society
Pablo Serrano Rodriguez, Alfred Sidney Barritt Iv, David Allen Gerber, Chirag Sureshchandra Desai
Patients with polycystic liver disease are described in the literature as both recipient and donor for liver transplant. Due to well-preserved liver function, it is often difficult for these patients to receive an organ. Livers of these patients are often large and heavier than a normal organ. We describe two cases who had exceedingly large livers, weighing 14 and 19 kg. To the best of our knowledge and search, these are some of the heaviest explanted livers, and one of the patients incidentally received a liver from a donor with ADPKD...
2018: Case Reports in Transplantation
Dennis Medved, Mattias Ohlsson, Peter Höglund, Bodil Andersson, Pierre Nugues, Johan Nilsson
The primary objective of this study is to compare the accuracy of two risk models, International Heart Transplantation Survival Algorithm (IHTSA), developed using deep learning technique, and Index for Mortality Prediction After Cardiac Transplantation (IMPACT), to predict survival after heart transplantation. Data from adult heart transplanted patients between January 1997 to December 2011 were collected from the UNOS registry. The study included 27,860 heart transplantations, corresponding to 27,705 patients...
February 26, 2018: Scientific Reports
Noriyuki Takahashi, Yoko Kuga, Satoko Uno, Keita Saito
A 75-year-old woman noticed a small mass in the right side breast and consulted our hospital. The results of the detailed examination indicated the synchronous double primary right breast cancer and the same side lung cancer (rS5). One-stage operation from the same skin incision was scheduled. Volume rendering (VR) of computed tomography (CT)-scan was very useful in deciding the position and the length of the skin incision. The breast tumor resection and the right middle lobe resection were successfully performed through 6...
February 2018: Kyobu Geka. the Japanese Journal of Thoracic Surgery
Yazhini Ravi, Srihari K Lella, Laurel A Copeland, Kiumars Zolfaghari, Kathleen Grady, Sitaramesh Emani, Chittoor B Sai-Sudhakar
BACKGROUND: Recipient-related factors, such as education level and type of health insurance, are known to affect heart transplantation outcomes. Pre-operative employment status has shown an association with survival in abdominal organ transplant patients. We sought to evaluate the effect of work status of heart transplant (HTx) recipients at the time of listing and at the time of transplantation on short- and long-term survival. METHODS: We evaluated the United Network for Organ Sharing (UNOS) registry for all adult HTx recipients from 2001 to 2014...
January 31, 2018: Journal of Heart and Lung Transplantation
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