Jacinta H Martin, Ilana R Bernstein, Jess M Lyons, Ariel R Brady, Nishani S Mabotuwana, Simone J Stanger, Camila Salum De Oliveira, Katerina B Damyanova, Brett Nixon, Tessa Lord
Oxygen availability can have profound effects on cell fate decisions and survival, in part by regulating expression of hypoxia-inducible factors (HIFs). In the ovary, HIF expression has been characterised in granulosa cells, however, any requirement in oocytes remains relatively undefined. Here we developed a Hif2a/Epas1 germline-specific knockout mouse line in which females were fertile, however produced 40% fewer pups than controls. No defects in follicle development were detected, and quality of MII oocytes was normal, as per assessments of viability, intracellular reactive oxygen species, and spindle parameters...
April 16, 2024: Scientific Reports