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https://www.readbyqxmd.com/read/28830076/copper-ii-bis-thiosemicarbazonato-complexes-as-anti-chlamydial-agents
#1
James W Marsh, Karrera Y Djoko, Alastair G McEwan, Wilhelmina M Huston
Lipophilic copper (Cu)-containing complexes have shown promising antibacterial activity against a range of bacterial pathogens. To examine the susceptibility of the intracellular human pathogen Chlamydia trachomatis to copper complexes containing bis(thiosemicarbazone) ligands [Cu(btsc)], we tested the in vitro effect of CuII-diacetyl- and CuII-glyoxal-bis[N(4)-methylthiosemicarbazonato] (Cu(atsm) and Cu(gtsm), respectively) on C. trachomatis. Cu(atsm) and to a greater extent, Cu(gtsm), prevented the formation of infectious chlamydial progeny...
September 29, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28829898/internal-amplification-control-of-pcr-for-the-glu1-dx5-allele-in-wheat
#2
H N Heim, E S N Vieira, L R T Polo, N K Lima, G J Silva, G A Linde, N B Colauto, I Schuster
One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in genetic breeding programs besides the loss of valuable genetic material. Thus, this study aimed to evaluate the viability of a microsatellite marker as an internal amplification control with a dominant marker for the wheat Glu1-Dx5 gene...
August 17, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28829835/exogenous-growth-factors-bfgf-egf-and-hgf-do-not-influence-viability-and-phenotype-of-v600ebraf-melanoma-cells-and-their-response-to-vemurafenib-and-trametinib-in-vitro
#3
Izabela Zalesna, Marta Osrodek, Mariusz L Hartman, Michal Rozanski, Malgorzata Sztiller-Sikorska, Karolina Niewinna, Dariusz Nejc, Malgorzata Czyz
It has been shown that the response of V600EBRAF melanoma cells to targeted therapeutics is affected by growth factors. We have investigated the influence of three different growth factors, bFGF, EGF and HGF used either alone or in combination, on the response of V600EBRAF melanoma cell populations established from surgical specimens to vemurafenib and trametinib, targeting V600EBRAF and MEK1/2, respectively. We report that proliferation and phenotype of V600EBRAF melanoma cell populations were not detectably influenced by exogenous growth factors...
2017: PloS One
https://www.readbyqxmd.com/read/28829830/nanofibrillar-cellulose-alginate-hydrogel-coated-surgical-sutures-as-cell-carrier-systems
#4
Patrick Laurén, Petter Somersalo, Irina Pitkänen, Yan-Ru Lou, Arto Urtti, Jouni Partanen, Jukka Seppälä, Mari Madetoja, Timo Laaksonen, Antti Mäkitie, Marjo Yliperttula
Hydrogel nanomaterials, especially those that are of non-human and non-animal origins, have great potential in biomedical and pharmaceutical sciences due to their versatility and inherent soft-tissue like properties. With the ability to simulate native tissue function, hydrogels are potentially well suited for cellular therapy applications. In this study, we have fabricated nanofibrillar cellulose-alginate (NFCA) suture coatings as biomedical devices to help overcome some of the limitations related to cellular therapy, such as low cell survivability and distribution out of target tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28829785/dendrimer-doxorubicin-conjugates-exhibit-improved-anticancer-activity-and-reduce-doxorubicin-induced-cardiotoxicity-in-a-murine-hepatocellular-carcinoma-model
#5
Sibu P Kuruvilla, Gopinath Tiruchinapally, A Colleen Crouch, Mohamed E H ElSayed, Joan M Greve
Hepatocellular carcinoma (HCC) is the 2nd leading cause of cancer-related deaths every year globally. The most common form of treatment, hepatic arterial infusion (HAI), involves the direct injection of doxorubicin (DOX) into the hepatic artery. It is plagued with limited therapeutic efficacy and the occurrence of severe toxicities (e.g. cardiotoxicity). We aim to improve the therapeutic index of DOX delivered via HAI by loading the drug onto generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers targeted to hepatic cancer cells via N-acetylgalactosamine (NAcGal) ligands...
2017: PloS One
https://www.readbyqxmd.com/read/28829562/facile-fabrication-and-characterization-of-a-pdms-derived-candle-soot-coated-stable-biocompatible-superhydrophobic-and-superhemophobic-surface
#6
Rameez Iqbal, Butunath Majhy, Ashis K Sen
We report a simple, inexpensive, rapid and one-step method for the fabrication of a stable and biocompatible superhydrophobic and superhemophobic surface. The proposed surface comprises candle soot particles embedded in a mixture of PDMS+n-hexane serving as the base material. The mechanism responsible for the superhydrophobic behavior of the surface is explained and the surface is characterized based on its morphology and elemental composition, wetting properties, mechanical and chemical stability and biocompatibility...
August 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28829507/microrna-613-attenuates-the-proliferation-migration-and-invasion-of-wilms-tumor-via-targeting-frs2
#7
H-F Wang, Y-Y Zhang, H-W Zhuang, M Xu
OBJECTIVE: Wilms' tumor is the most common malignant tumor in children worldwide. Considering the poor therapeutic effect on Wilms' tumor, we determined the effects of microRNA-613 on cell proliferation and metastasis in vitro, providing therapeutic targets for the treatment of Wilms' tumor. PATIENTS AND METHODS: Quantitative real-time PCR (qRT-PCR) was employed to identify the expression level of miR-613. CCK8 and colony formation assays were incorporated to assess cell viability and proliferation capacity...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829505/dnmt3a-mediated-down-regulation-of-microrna-105-promotes-gastric-cancer-cell-proliferation
#8
G-Q Zhou, F Han, Z-L Shi, L Yu, X-F Li, C Yu, C-L Shen, D-W Wan, X-G Zhu, R Li, S-B He
OBJECTIVE: It has been well-established that microRNAs (miRNAs), a class of short non-coding RNA molecules, play an important role in the development of gastric cancer. In the present study, we focused on miR-105, a novel miRNA not previously linked to gastric cancer. PATIENTS AND METHODS: 36 paired surgically resected gastric cancer tissues and matched adjacent normal tissues were used to detect the expression of miR-105. AGS cells were used to overexpress or silence of miR-105 and to determine its effect on several tumorigenic properties...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829409/surgical-angiogenesis-in-porcine-tibial-allotransplantation-a-new-large-animal-bone-vascularized-composite-allotransplantation-model
#9
Dimitra Kotsougiani, Caroline A Hundepool, Joost I Willems, Patricia Friedrich, Alexander Y Shin, Allen T Bishop
Segmental bone loss resulting from trauma, infection malignancy and congenital anomaly remains a major reconstructive challenge. Current therapeutic options have significant risk of failure and substantial morbidity. Use of bone vascularized composite allotransplantation (VCA) would offer both a close match of resected bone size and shape and the healing and remodeling potential of living bone. At present, life-long drug immunosuppression (IS) is required. Organ toxicity, opportunistic infection and neoplasm risks are of concern to treat such non-lethal indications...
August 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28829358/impact-of-platelet-rich-plasma-on-viability-and-proliferation-in-wound-healing-processes-after-external-radiation
#10
Yvonne Reinders, Oliver Felthaus, Gero Brockhoff, Fabian Pohl, Norbert Ahrens, Lukas Prantl, Frank Haubner
Platelet-rich plasma is a current subject of studies on chronic wound healing therapy due to possible pro-angiogenic effects. Microvascular compromise represents the major component in radiogenic wound healing complications. The effects of platelet-rich plasma on irradiated cells of the cutaneous wound healing process are poorly understood so far. In this study, the interaction of endothelial cells and adipose-derived stem cells in conjunction with treatment with platelet-rich plasma is investigated in the context of radiation effects...
August 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28828742/a-synthetic-cannabinoid-jwh-210-reduces-lymphoid-organ-weights-and-t-cell-activator-levels-in-mice-via-cb2-receptors
#11
Sun Mi Gu, Hyun Jin Lee, Tac-Hyung Lee, Yun Jeong Song, Young-Hoon Kim, Kyoung-Moon Han, Jisoon Shin, Hye-Kyung Park, Hyung Soo Kim, Hye Jin Cha, Jaesuk Yun
The problem of new psychoactive substances (NPS) is emerging globally. However, the immunotoxicity of synthetic cannabinoids is not evaluated extensively yet. The purpose of the present study was to investigate whether synthetic cannabinoids (JWH-210 and JWH-030) induce adverse effects on lymphoid organs, viability of splenocytes and thymocytes, and immune cell activator and cytokines in mice. JWH-210 (10 mg/kg, 3 days, i.p.) is more likely to have cytotoxicity and reduce lymphoid organ weight than JWH-030 of ICR mice in vivo...
August 22, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28828705/glucotoxicity-promotes-aberrant-activation-and-mislocalization-of-ras-related-c3-botulinum-toxin-substrate-1-rac1-and-metabolic-dysfunction-in-pancreatic-islet-%C3%AE-cells-reversal-of-such-metabolic-defects-by-metformin
#12
Sartaj Baidwan, Anil Chekuri, DiAnna L Hynds, Anjaneyulu Kowluru
Emerging evidence suggests that long-term exposure of insulin-secreting pancreatic β-cells to hyperglycemic (HG; glucotoxic) conditions promotes oxidative stress, which, in turn, leads to stress kinase activation, mitochondrial dysfunction, loss of nuclear structure and integrity and cell apoptosis. Original observations from our laboratory have proposed that Rac1 plays a key regulatory role in the generation of oxidative stress and downstream signaling events culminating in the onset of dysfunction of pancreatic β-cells under the duress of metabolic stress...
August 21, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28828622/modafinil-attenuates-inflammation-via-inhibiting-akt-nf-%C3%AE%C2%BAb-pathway-in-apoe-deficient-mouse-model-of-atherosclerosis
#13
Jinxia Han, Dongwei Chen, Dayi Liu, Yanan Zhu
Modafinil, an FDA approved wakefulness drug prescribed to narcolepsy patients, has recently been shown to have anti-inflammatory effects and provides protection against neuroinflammation. It is unknown if modafinil can also protect against atherosclerosis, pathogenesis of which implicates inflammation. Using an apoE-deficient mouse model, we tried to elucidate the effects of modafinil treatment on the development of atherosclerosis. We tested serum levels of cytokines. We isolated mouse bone marrow-derived macrophages (BMDMs), detected effect of modafinil on the viability and proliferation of BMDMs, and on oxidized low-density lipoprotein-induced IL-6 and TNF-α, and supernatant level of IFN-γ as well as NF-κB activity in BMDMs...
August 21, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28828504/tissue-engineering-relevant-characteristics-of-ex-vivo-and-monolayer-expanded-chondrocytes-from-the-notch-versus-trochlea-of-human-knee-joints
#14
Matthias Aurich, Gunther Olaf Hofmann, Bernd Rolauffs
PURPOSE: The aim was to analyse the biological characteristics of chondrocytes from the two biopsy sites notch vs. trochlea of human knee joints. The question was whether tissue engineering-relevant characteristics such as viability and mRNA expression profile would be comparable ex vivo and after monolayer expansion, as these are parts of routine autologous chondrocyte implantation (ACI). METHODS: Biopsies from the intercondylar notch and the lateral aspect of the trochlea from 20 patients with ICRS grades 3 and 4 cartilage defects were harvested during arthroscopy...
August 21, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28828260/preparation-and-characteristics-of-gelatin-sponges-crosslinked-by-microbial-transglutaminase
#15
Haiyan Long, Kunlong Ma, Zhenghua Xiao, Xiaomei Ren, Gang Yang
Microbial transglutaminase (mTG) was used as a crosslinking agent in the preparation of gelatin sponges. The physical properties of the materials were evaluated by measuring their material porosity, water absorption, and elastic modulus. The stability of the sponges were assessed via hydrolysis and enzymolysis. To study the material degradation in vivo, subcutaneous implantations of sponges were performed on rats for 1-3 months, and the implanted sponges were analyzed. To evaluate the cell compatibility of the mTG crosslinked gelatin sponges (mTG sponges), adipose-derived stromal stem cells were cultured and inoculated into the scaffold...
2017: PeerJ
https://www.readbyqxmd.com/read/28828244/freeze-thaw-decellularization-of-the-trabecular-meshwork-in-an-ex-vivo-eye-perfusion-model
#16
Yalong Dang, Susannah Waxman, Chao Wang, Adrianna Jensen, Ralitsa T Loewen, Richard A Bilonick, Nils A Loewen
OBJECTIVE: The trabecular meshwork (TM) is the primary substrate of outflow resistance in glaucomatous eyes. Repopulating diseased TM with fresh, functional TM cells might be a viable therapeutic approach. Decellularized TM scaffolds have previously been produced by ablating cells with suicide gene therapy or saponin, which risks incomplete cell removal or dissolution of the extracellular matrix, respectively. We hypothesized that improved trabecular meshwork cell ablation would result from freeze-thaw cycles compared to chemical treatment...
2017: PeerJ
https://www.readbyqxmd.com/read/28828227/a-novel-p-t139m-mutation-in-hspb1-highlighting-the-phenotypic-spectrum-in-a-family
#17
Jakkrit Amornvit, Mehmet E Yalvac, Lei Chen, Zarife Sahenk
INTRODUCTION: Mutations in the HSPB1 gene encoding the small heat shock protein B1 are associated with an autosomal dominant, axonal form of Charcot-Marie-Tooth disease 2F (CMT2F) and distal hereditary motor neuropathy. Recently, distal myopathy had been described in a patient carrying HSPB1 mutation adding to the complexity of phenotypes resulting from HSPB1 mutations. METHODS: Five patients in a family with concerns of hereditary neuropathy were included. Detailed clinical examinations, including assessments of motor and sensory function, and electrophysiological data were obtained...
August 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28827996/apigenin-7-o-glucoside-versus-apigenin-insight-into-the-modes-of-anticandidal-and-cytotoxic-actions
#18
Marija Smiljkovic, Danijela Stanisavljevic, Dejan Stojkovic, Isidora Petrovic, Jelena Marjanovic Vicentic, Jelena Popovic, Simona Golic Grdadolnik, Dejan Markovic, Snežana Sankovic-Babice, Jasmina Glamoclija, Milena Stevanovic, Marina Sokovic
Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin-7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28827967/in-vitro-antidiabetic-activity-of-polar-and-nonpolar-solvent-extracts-from-leucas-aspera-willd-link-leaves
#19
V M Annapandian, R Shanmuga Sundaram
BACKGROUND: Diabetes mellitus is a chronic illness, and the management of diabetes is a global problem. Successful treatment is required to prevent complications and organ damages. Herbal medicines are having minimal adverse effects when compared to the available synthetic drugs to treat such chronic diseases and disorders. OBJECTIVE: The present study was aimed to evaluate the antidiabetic and antioxidant activity of polar and nonpolar solvent extracts of Leucas aspera (Willd...
July 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28827850/platelet-poor-plasma-as-a-supplement-for-fibroblasts-cultured-in-platelet-rich-fibrin
#20
Luiz Alexandre Chisini, Sarah Arangurem Karam, Thaís Gioda Noronha, Letícia Regina Morello Sartori, Alissa Schmidt San Martin, Flávio Fernando Demarco, Marcus Cristian Muniz Conde
The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)(2) to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x10(4) cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days...
June 2017: Acta Stomatologica Croatica
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