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https://www.readbyqxmd.com/read/28222333/immunoprofiling-as-a-predictor-of-patient-s-response-to-cancer-therapy-promises-and-challenges
#1
REVIEW
Daniel Bethmann, Zipei Feng, Bernard A Fox
Immune cell infiltration is common to many tumors and has been recognized by pathologists for more than 100 years. The application of digital imaging and objective assessment software allowed a concise determination of the type and quantity of immune cells and their location relative to the tumor and, in the case of colon cancer, characterized overall survival better than AJCC TNM staging. Subsequently, expression of PD-L1, by 50% or more tumor cells, identified NSCLC patients with double the response rate to anti-PD-1...
February 18, 2017: Current Opinion in Immunology
https://www.readbyqxmd.com/read/28222253/flow-cytometry-based-rapid-duplexed-immunoassay-for-fusarium-mycotoxins
#2
Árpád Czéh, Miklós Mézes, Francis Mandy, Zsuzsanna Szőke, György Nagyéri, Noémi Laufer, Balázs Kőszegi, Tamás Koczka, Sándor Kunsági-Máté, György Lustyik
At small food processing facilities, the most frequently used test to determine if grain-derived mycotoxin concentrations are compliant with legal limits is the enzyme-linked immunosorbent assay (ELISA). Each kit is designed to detect one of the six dangerous mycotoxins. With the increasing occurrence of coinfection of grain with multiple-mycotoxins in the field and/or during storage, ELISA is no longer a cost effective best assay option. With ELISA, each species of mycotoxin requires different sample preparation/extraction and a 45 min incubation...
February 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28221989/evaluation-of-a-multiplex-pcr-assay-for-the-identification-of-salmonella-serovars-enteritidis-and-typhimurium-using-retail-and-abattoir-samples
#3
Dele Ogunremi, Susan Nadin-Davis, Andrée Ann Dupras, Imelda Gálvan Márquez, Katayoun Omidi, Louise Pope, John Devenish, Teresa Burke, Ray Allain, Daniel Leclair
A multiplex PCR was developed to identify the two most common serovars of Salmonella causing foodborne illness in Canada, namely, serovars Enteritidis and Typhimurium. The PCR was designed to amplify DNA fragments from four Salmonella genes, namely, invA gene (211-bp fragment), iroB gene (309-bp fragment), Typhimurium STM 4497 (523-bp fragment), and Enteritidis SE147228 (612-bp fragment). In addition, a 1,026-bp ribosomal DNA (rDNA) fragment universally present in bacterial species was included in the assay as an internal control fragment...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221870/bovine-leukemia-virus-and-mycobacterium-avium-subsp-paratuberculosis-are-not-associated-with-shiga-toxin-producing-escherichia-coli-shedding-in-cattle
#4
Cristina Venegas-Vargas, Shannon D Manning, Paul M Coussens, Jonathan A Roussey, Paul Bartlett, Daniel Grooms
Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis in cattle, and Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in cattle. Both diseases are chronic in nature and can lead to the disruption of normal immunological or physiological processes. Cattle are the major reservoir of Shiga toxin-producing Escherichia coli (STEC), a cause of foodborne illness in humans. We tested the hypothesis that cattle infected with BLV or MAP are more likely to shed STEC...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221751/molecular-engineering-and-design-of-semiconducting-polymer-dots-with-narrow-band-near-infrared-emission-for-in-vivo-biological-imaging
#5
Chi-Shiang Ke, Chia-Chia Fang, Jia-Ying Yan, Po-Jung Tseng, Joseph R Pyle, Chuan-Pin Chen, Shu-Yi Lin, Jixin Chen, Xuanjun Zhang, Yang-Hsiang Chan
This article describes the design and synthesis of donor-bridge-acceptor based semiconducting polymer dots (Pdots) that exhibit narrow-band emissions, ultrahigh brightness, and large Stokes shifts in the near-infrared (NIR) region. We systematically investigated the effect of π-bridges on the fluorescence quantum yields of the donor-bridge-acceptor based Pdots. The Pdots could be excited by a 488-nm or 532-nm laser and have a high fluorescence quantum yield of 33% with a Stokes shift over 200 nm. The emission full width at half-maximum of the Pdots can be as narrow as 29 nm, about 2...
February 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28221712/discovery-of-myh14-as-an-important-and-unique-deafness-gene-causing-prelingually-severe-autosomal-dominant-non-syndromic-hearing-loss
#6
Bong Jik Kim, Ah Reum Kim, Jin Hee Han, Chung Lee, Doo Yi Oh, Byung Yoon Choi
BACKGROUND: Pathogenic variants of MYH14 have been known to be associated-in either a syndromic or non-syndromic manner-with hearing loss. Interestingly, all reported cases to date of MYH14-related non-syndromic hearing loss with detailed phenotypes have demonstrated mild-to-moderate progressive hearing loss with postlingual onset. METHODS: In this study, targeted resequencing (TRS) of known deafness genes was performed to identify the causative variant in two multiplex families segregating AD inherited hearing loss...
February 21, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28220719/protector-effect-of-%C3%AE-thalassaemia-on-cholecystitis-and-cholecystectomy-in-sickle-cell-disease
#7
Robéria M Pontes, Elaine S Costa, Patrícia F R Siqueira, Jussara F F Medeiros, Andréa Soares, Fabiana V de Mello, Maria C Maioli, Isaac L S Filho, Liliane R Alves, Marcelo G P Land, Marcos K Fleury
OBJECTIVES: Cholecystitis is one of the complications of symptomatic cholelithiasis responsible for high levels of morbidity of sickle cell disease (SCD) patients. Here, we investigated the possible protective role of single gene deletions of α-thalassaemia in the occurrence of cholelithiasis and cholecystitis in SCD patients, as well as the cholecystectomy requirements. METHODS: The α-globin genotype was determined in 83 SCD patients using the multiplex-polymerase chain reaction and compared with clinical events...
February 21, 2017: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/28220435/rapid-construction-of-multiplexed-crispr-cas9-systems-for-plant-genome-editing
#8
Levi Lowder, Aimee Malzahn, Yiping Qi
Multiplex CRISPR-Cas9 nuclease mediated genome editing is an efficient method for disrupting gene function in plants. Use of CRISPR-Cas9 has escalated rapidly in recent years and is expected to become routine practice in molecular biology and related fields of research. Due to the relatively novel and widespread adoption of this technology, first-time users may not have regular access to experienced guidance or technical support from peers or mentors. Here, we offer guidance and technical support in the form of a detailed and tested protocol for simultaneous targeting of three separate loci on the TRANSPARENT TESTA 4 (TT4) gene in Arabidopsis thaliana using multiplex CRISPR-Cas9...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220270/multiplex-assessment-of-serum-cytokine-and-chemokine-levels-in-idiopathic-morphea-and-vitamin-k1-induced-morphea
#9
Lori Ann Cox, Guy F Webster, Sonsoles Piera-Velazquez, Sergio A Jimenez
The levels of 63 cytokines, chemokines, and growth factors were measured in the serum of four patients with idiopathic morphea and of one patient with vitamin K1-induced morphea employing a multiplex assay to identify the role of inflammatory/immunologic events in their pathogenesis. Full-thickness skin biopsies of affected skin were analyzed by histopathology. Luminex assays for 63 cytokines, chemokines, and growth factors were performed in the sera from four patients with idiopathic morphea and in two different samples of serum obtained in two separate occasions from one patient with vitamin K1-induced morphea...
February 20, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28220018/germline-variants-in-familial-pituitary-tumour-syndrome-genes-are-common-in-young-patients-and-families-with-additional-endocrine-tumours
#10
Sunita Mc De Sousa, Mark J McCabe, Kathy Wu, Tony Roscioli, Velimir Gayevskiy, Katelyn Brook, Lesley Rawlings, Hamish S Scott, Tanya J Thompson, Peter Earls, Anthony J Gill, Mark J Cowley, Marcel E Dinger, Ann I McCormack
Objective: Familial pituitary tumour syndromes (FPTS) account for 5% of pituitary adenomas. Multi-gene analysis via next generation sequencing (NGS) may unveil greater prevalence and inform clinical care. We aimed to identify germline variants in selected patients with pituitary adenomas using a targeted NGS panel. Design: We undertook a nation-wide cross-sectional study of patients with pituitary adenomas with onset ≤40 years of age and/or other personal/family history of endocrine neoplasia. A custom NGS panel was performed on germline DNA to interrogate eight FPTS genes...
February 20, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28219127/-sanger-sequencing-for-the-diagnosis-of-spinal-muscular-atrophy-patients-with-survival-motor-neuron-gene-1-compound-heterozygous-mutation
#11
L Yang, Y Y Cao, Y J Qu, J L Bai, H Wang, Y W Jin, Y L Han, F Song
Objective: To detect the subtle variant of survival motor neuron gene 1(SMN1) by Sanger sequencing, and to assess the value of Sanger sequencing for the diagnosis of spinal muscular atrophy(SMA) with compound heterozygous mutation of SMN1. Methods: Fifty-two patients suspected SMA were recruited by the Capital Institute of Pediatrics from Jan.2014 to June.2016. PCR was used for amplifying exon7 of SMN1 and SMN2 in 52 patients. Natural different base peaks on the sequencing chromatogram in the SMN1 and SMN2 within the amplified segments were identified with Sanger DNA sequencing to detect the homozygous deletion or heterozygous deletion of SMN1...
February 14, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28218288/detection-of-viral-respiratory-pathogens-in-mild-and-severe-acute-respiratory-infections-in-singapore
#12
Lili Jiang, Vernon Jian Ming Lee, Lin Cui, Raymond Lin, Chyi Lin Tan, Linda Wei Lin Tan, Wei-Yen Lim, Yee-Sin Leo, Louie Low, Martin Hibberd, Mark I-Cheng Chen
To investigate the performance of laboratory methods and clinical case definitions in detecting the viral pathogens for acute respiratory infections (ARIs) from a prospective community cohort and hospital inpatients, nasopharyngeal swabs from cohort members reporting ARIs (community-ARI) and inpatients admitted with ARIs (inpatient-ARI) were tested by Singleplex Real Time-Polymerase Chain Reaction (SRT-PCR), multiplex RT-PCR (MRT-PCR) and pathogen-chip system (PathChip) between April 2012 and December 2013...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28218042/doxorubicin-has-a-synergistic-cytotoxicity-with-cucurbitacin-b-in-anaplastic-thyroid-carcinoma-cells
#13
Si Hyoung Kim, Jun Goo Kang, Chul Sik Kim, Sung-Hee Ihm, Moon Gi Choi, Hyung Joon Yoo, Seong Jin Lee
In this study, the combined effect of doxorubicin with cucurbitacin B on survival of anaplastic thyroid carcinoma cells was evaluated. For experiments, 8505C and CAL62 human anaplastic thyroid carcinoma cells were used. Cell viability, the percentage of viable cells, and cytotoxic activity were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, multiplexed cytotoxicity assay, and cytotoxicity assay, respectively. Reactive oxygen species production was measured. In experiments, doxorubicin and cucurbitacin B reduced cell viability in a dose- and time-dependent manner...
February 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28216667/a-novel-hrm-assay-for-the-simultaneous-detection-and-differentiation-of-eight-poxviruses-of-medical-and-veterinary-importance
#14
Esayas Gelaye, Lukas Mach, Jolanta Kolodziejek, Reingard Grabherr, Angelika Loitsch, Jenna E Achenbach, Norbert Nowotny, Adama Diallo, Charles Euloge Lamien
Poxviruses belonging to the Orthopoxvirus, Capripoxvirus and Parapoxvirus genera share common host species and create a challenge for diagnosis. Here, we developed a novel multiplex PCR method for the simultaneous detection and differentiation of eight poxviruses, belonging to three genera: cowpox virus (CPXV) and camelpox virus (CMLV) [genus Orthopoxvirus]; goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV) [genus Capripoxvirus]; orf virus (ORFV), pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) [genus Parapoxvirus]...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216437/extracellular-eosinophilic-traps-in-association-with-staphylococcus-aureus-at-the-site-of-epithelial-barrier-defects-in-severe-airway-inflammation
#15
Elien Gevaert, Nan Zhang, Olga Krysko, Feng Lan, Gabriële Holtappels, Natalie De Ruyck, Hans Nauwynck, Shida Yousefi, Hans-Uwe Simon, Claus Bachert
BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a Th2-biased eosinophilic inflammation. Eosinophils have been shown to generate so-called extracellular eosinophilic traps (EETs) under similar pathological conditions. OBJECTIVE: Our aim was to investigate a possible link between EET formation and the presence of Staphylococcus aureus, an organism frequently colonizing the upper airways, at the human mucosal site of the disease. METHODS: Tissue slides were investigated for the presence of EETs and S...
February 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28214747/immunorecognition-magnetic-supports-for-the-development-of-an-electrochemical-immunoassay-for-azaspiracid-detection-in-mussels
#16
Sandra Leonardo, Maria Rambla-Alegre, Ingunn A Samdal, Christopher O Miles, Jane Kilcoyne, Jorge Diogène, Ciara K O'Sullivan, Mònica Campàs
As azaspiracids (AZAs) are being reported from the coastal waters of an increasing number of countries on a global scale, the need for rapid, simple and cost-effective methods to detect these marine toxins and protect seafood consumers' health is becoming evident. A magnetic bead (MB)-based direct immunoassay for the detection of AZAs, using protein G-coated MBs as supports for antibody immobilisation and peroxidase-labelled AZA as a tracer is detailed. A colorimetric approach was first developed to optimise the experimental parameters and establish the cross-reactivity factors for AZA-1-10...
February 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28214223/evaluation-of-a-field-test-for-antibodies-against-chlamydia-trachomatis-during-trachoma-surveillance-in-nepal
#17
Michelle J Sun, Andrea I Zambrano, Laura Dize, Beatriz Munoz, Sarah Gwyn, Sailesh Mishra, Diana L Martin, Shekhar Sharma, Sheila K West
PURPOSE: Testing for antibodies to Chlamydia trachomatis has potential as a surveillance tool. Our evaluation compares lateral flow assays (LFAs) during surveillance surveys in Nepal with Multiplex bead array (MBA). Fifty children were randomly sampled from each of 15 random clusters in two districts of Nepal. Finger prick blood samples were collected from 1509 children and tested onsite for anti-Pgp3 antibodies by LFA. The LFA was read at 30min as negative, positive, or invalid. Tests results were also rated as difficult to read ("equivocal")...
January 17, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28214222/necessity-to-critically-review-the-automatic-results-of-the-xpert-flu-assay
#18
Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Anny Dewilde, Didier Hober
While using the Xpert Flu assay we became aware of false-negative results. The study aimed to analyze the causes of these false-negative results. One hundred fifty-nine respiratory specimens were tested in the Xpert Flu assay and in multiplex reverse transcription-polymerase chain reactions (RT-PCRs) for respiratory viruses. Discordant specimens were tested in the Influenza A/B r-gene assay. One hundred fifty-two (96%) and 151 (95%) specimens yielded concordant results for influenza A and B, respectively. Fifteen specimens tested negative in the Xpert Flu assay and positive in a multiplex RT-PCR...
February 2, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28213965/comparison-of-culture-single-and-multiplex-real-time-pcr-for-detection-of-sabin-poliovirus-shedding-in-recently-vaccinated-indian-children
#19
Sidhartha Giri, Anand K Rajan, Nirmal Kumar, Pavithra Dhanapal, Jayalakshmi Venkatesan, Miren Iturizza-Gomara, Mami Taniuchi, Jacob John, Asha Mary Abraham, Gagandeep Kang
Although culture is considered the gold standard for poliovirus detection from stool samples, real-time PCR has emerged as a faster and more sensitive alternative. Detection of poliovirus from the stool of recently vaccinated children by culture, single and multiplex real-time PCR was compared. Of the 80 samples tested, 55 (68.75%) were positive by culture compared to 61 (76.25%) and 60 (75%) samples by the single and one step multiplex real-time PCR assays respectively. Real-time PCR (singleplex and multiplex) is more sensitive than culture for poliovirus detection in stool, although the difference was not statistically significant...
February 18, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28213879/arthrogryposis-multiplex-congenita-classification-diagnosis-perioperative-care-and-anesthesia
#20
REVIEW
Lulu Ma, Xuerong Yu
Arthrogryposis multiplex congenita (AMC) is a rare disorder characterized by non-progressive, multiple contractures. In addition to affected extremities, patients may also present microstomia, decreased temporomandibular joint mobility. Although the etiology of AMC is unclear, any factor that decreases fetal movement is responsible for AMC. Thus, accurate diagnosis and classification are crucial to the appropriate treatment of AMC. The development of ultrasound technology has enabled prenatal diagnosis. Very early treatment is favorable, and multidisciplinary treatment is necessary to improve the function of AMC patients...
February 17, 2017: Frontiers of Medicine
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