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https://www.readbyqxmd.com/read/29166639/apobec3a-expression-in-penile-squamous-cell-carcinoma
#1
Martina Heller, Elena-Sophie Prigge, Adam Kaczorowski, Magnus von Knebel Doeberitz, Markus Hohenfellner, Stefan Duensing
BACKGROUND: APOBECs (apolipoprotein B mRNA-editing catalytic polypeptides) are cytidine deaminases that have been implicated in the host defense against viruses by blocking viral replication. They have also been shown to play a role in genome hypermutation in several human cancers. An APOBEC3 hypermutation signature has been discovered in cervical cancer, which is intimately associated with infection by high-risk human papillomaviruses (HPVs). At the same time, HPV genomes themselves are subject to DNA editing by APOBECs...
November 23, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29166104/rapid-spectro-polarimetry-to-probe-molecular-symmetry-in-multiplex-coherent-anti-stokes-raman-scattering
#2
Thomas Würthwein, Maximilian Brinkmann, Tim Hellwig, Carsten Fallnich
We present the simultaneous detection of the spectrum and the complete polarization state of a multiplex coherent anti-Stokes Raman scattering signal with a fast division-of-amplitude spectro-polarimeter. The spectro-polarimeter is based on a commercial imaging spectrograph, a birefringent wedge prism, and a segmented polarizer. Compared to the standard rotating-retarder fixed-analyzer spectro-polarimeter, only a single measurement is required and an up to 21-fold reduced acquisition time is shown. The measured Stokes parameters allow us to differentiate between vibrational symmetries and to determine the depolarization ratio ρ by data post-processing...
November 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29165917/a-novel-multisite-confocal-system-for-rapid-ca-2-imaging-from-submicron-structures-in-brain-slices
#3
Luiza Filipis, Karima Ait Ouares, Philippe Moreau, Dimitrii Tanese, Valeria Zampini, Andrea Latini, Chun Bleau, Charlie Bleau, Jeremy Graham, Marco Canepari
In brain slices, resolving fast Ca(2+) fluorescence signals from submicron structures is typically achieved using two-photon or confocal scanning microscopy, an approach that limits the number of scanned points. The novel multiplexing confocal system presented here overcomes this limitation. This system is based on a fast spinning disk, a multimode diode laser and a novel high-resolution CMOS camera. The spinning disk, running at 20,000 rpm, has custom-designed spiral pattern that maximises light collection while rejecting out-of-focus fluorescence to resolve signals from small neuronal compartments...
November 22, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29165905/loop-mediated-isothermal-amplification-lamp-a-versatile-technique-for-detection-of-microorganisms
#4
REVIEW
Yien-Ping Wong, Shuhaidah Othman, Yee-Ling Lau, Radu Son, Hui-Yee Chee
Loop mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand activity and a set of specifically designed primers to amplify targeted DNA strands. Following its first discovery by Notomi et al. in the year 2000, LAMP was further developed over the years which involved the combination of this technique with other molecular approaches, such as reverse transcription and multiplex amplification for the detection of infectious diseases caused by microorganisms in humans, livestock and plants...
November 22, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29165496/fluorescence-quenching-based-signal-amplification-on-immunochromatography-test-strips-for-dual-mode-sensing-of-two-biomarkers-of-breast-cancer
#5
Bo Zhang, Wenjuan Ma, Fuxin Li, Weichen Gao, Qian Zhao, Weipan Peng, Jiafang Piao, Xiaoli Wu, Hanjie Wang, Xiaoqun Gong, Jin Chang
Recently, immunochromatography test strips (ICTS) have been fully developed for point-of-care testing (POCT). However, the intrinsic limitations including non-quantitative detection of colloidal gold ICTS and low sensitivity of fluorescence ICTS (FICTS) significantly restrict their further application in clinical diagnosis. Taking advantages of rapid colorimetric qualitative detection and fluorescence quantitation, we designed a kind of sensitive and dual-mode magnetic FICTS (mFICTS) based on PLGA@Fe3O4 super-paramagnetic nanosphere (SPMN) probes quenching multiplex fluorescer on the test line through sandwich immunoreactions...
November 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/29165491/fe3o4-aunps-anchored-2d-metal-organic-framework-nanosheets-with-dna-regulated-switchable-peroxidase-like-activity
#6
Bing Tan, Huimin Zhao, Weihao Wu, Xuan Liu, Yaobin Zhang, Xie Quan
Two-dimensional (2D) metal-organic framework (MOF) nanosheets emerging as a new member of the 2D family have received significant research interest in recent years. Herein, we have successfully synthesized 2D copper-based MOF nanosheets with bimetallic anchorage using a facile two-step process at room temperature and ambient pressure, denoted as Cu(HBTC)-1/Fe3O4-AuNPs nanosheets. The as-synthesized 2D bimetallic MOF nanosheets displayed enhanced peroxidase-like activity with relatively high catalytic velocity and affinity for substrates compared with previously reported peroxidase mimics...
November 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/29164400/hepatitis-c-virus-infection-among-illicit-drug-users-in-an-archipelago-of-the-amazon
#7
Fabricio Q Silva, Francisco J A Santos, Andreia P Andrade, Suzy D B Pacheco, Benedikt Fischer, João Renato R Pinho, José Alexandre R Lemos, Aldemir B Oliveira-Filho
Hepatitis C virus (HCV) infection causes acute and chronic liver disease and may lead to cirrhosis, liver failure or hepatocellular carcinoma. The behavior of illicit drug users (DUs) typically exposes them to risks of viral infection. In the Brazilian Amazon region, a number of studies have identified high rates of drug use among adolescents, and a high prevalence of HBV infection in DUs, disseminated by sexual and parenteral activities. However, the epidemiological scenario of HCV infection in the region is still poorly understood...
November 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29164226/nicking-enzyme-controlled-toehold-regulation-for-dna-logic-circuits
#8
Linqiang Pan, Zhiyu Wang, Yifan Li, Fei Xu, Qiang Zhang, Cheng Zhang
DNA strand displacement is widely used in DNA-related nanoengineering for its remarkable specificity and predictability. We report a nicking enzyme-assisted mechanism to regulate strand displacement, where DNA toeholds are dynamically controlled. To demonstrate the strategy, a protein/DNA-based Boolean operation system is constructed and based on it a two-channel multiplexer controlled by three different nicking enzymes is realized. The proposed regulatory mechanism can be used for switch logic statement and bridges protein and DNA logic circuits...
November 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/29164044/characterization-of-clostridium-difficile-strains-isolated-from-patients-with-c-difficile-associated-disease-in-korea
#9
Seung-Hak Cho, Jung-Whan Chon, Kun-Ho Seo, Young Kwon Kim, Jung-Beom Kim, Young-Seok Bak, Woon-Won Jung, Cheorl-Ho Kim, Jong Tae Choi
Objectives: Studies on Clostridium difficile are rare in Korea. We investigated the epidemiological characteristics of C. difficile isolates from patients with C. difficile-associated disease (CDAD) in Korea. Methods: Multiplex polymerase chain reaction was performed to detect the presence of tcdA and tcdB toxin genes. Antimicrobial susceptibility test was carried out by the disk-dilution method. C. difficile strains were subtyped by automated repetitive-element palindromic PCR (rep-PCR)...
October 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/29163767/immunology-repertoire-study-of-pulmonary-sarcoidosis-t-cells-in-cd4-cd8-pbmc-and-tissue
#10
Yingyun Fu, Yazhen Li, Lan Xu, Shengguo Liu, Minlian Wang, Lu Xiao, Song Liu, Yong Dai
Sarcoidosis is a systemic granulomatous disorder highly related with immune response. The diversity and stability of the immune system could be measured by hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (TCR). Here we used a combination of multiplex PCR and next-generation sequencing to conduct a good quality analysis of the T-cell receptor BV complementarity-determining region 3 (TCR BV CDR3) gene in peripheral blood mononuclear cells (PBMCs) from 7 sarcoidosis patients and lung sarcoidosis tissue from 6 patients...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163651/molecular-detection-of-virulence-genes-and-multi-drug-resistance-patterns-in-escherichia-coli-stec-in-clinical-bovine-mastitis-alborz-province-iran
#11
M Tavakoli, H Pourtaghi
The aim of this study was to identify virulence genes and antimicrobial resistance of Escherichia coli isolated from bovine clinical mastitis in dairy herds in Iran. Sampling was done from 86 inflamed quarters of dairy cows in 8 commercial farms of Alborz province, Iran in summer 2015. Shiga toxin-producing E. coli (STEC) virulence genes were detected by multiplex PCR and multi-drug resistance profiles were confirmed using disk diffusion method. Among 60 E. coli isolated from examined samples, 13 (21.6%) of them were STEC...
2017: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/29163646/prevalence-of-campylobacter-jejuni-and-campylobacter-coli-in-captive-wildlife-species-of-india
#12
A A Prince Milton, R K Agarwal, G B Priya, M Saminathan, M Aravind, A Reddy, C K Athira, Anjay, T P Ramees, K Dhama, A K Sharma, A Kumar
Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals' food and water were collected from G. B. Pant High Altitude Zoo, Nainital, Kanpur Zoo, Wildlife Park, IVRI and the Post Graduate Research Institute in Animal Sciences (PGRIAS), Chennai, Tamilnadu from August 2014 to May 2015...
2017: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/29163543/interleukin-6-present-in-inflammatory-ascites-from-advanced-epithelial-ovarian-cancer-patients-promotes-tumor-necrosis-factor-receptor-2-expressing-regulatory-t-cells
#13
Nirmala Chandralega Kampan, Mutsa Tatenda Madondo, Orla M McNally, Andrew N Stephens, Michael A Quinn, Magdalena Plebanski
Background: Epithelial ovarian cancer (EOC) remains a highly lethal gynecological malignancy. Ascites, an accumulation of peritoneal fluid present in one-third of patients at presentation, is linked to poor prognosis. High levels of regulatory T cells (Tregs) in ascites are correlated with tumor progression and reduced survival. Malignant ascites harbors high levels of Tregs expressing the tumor necrosis factor receptor 2 (TNFR2), as well as pro-inflammatory factors such as interleukin 6 (IL-6) and tumor necrosis factor (TNF)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163387/a-multiplex-sybr-green-real-time-pcr-assay-for-the-detection-of-three-colistin-resistance-genes-from-cultured-bacteria-feces-and-environment-samples
#14
Jiyun Li, Xiaomin Shi, Wenjuan Yin, Yang Wang, Zhangqi Shen, Shuangyang Ding, Shaolin Wang
The aim of the study was to develop a multiplex assay for rapid detection of mcr-1, mcr-2, and mcr-3, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the mcr genes, and applied to cultured bacteria, feces and soil samples. All three mcr genes could be detected with a lower limit of 10(2) cultured bacteria. This test was highly specific and sensitive, and generated no false-positive results. The assay was also conclusive when applied to feces and soil samples containing mcr-1-positive Escherichia coli, which could facilitate the screening of mcr genes not only in the bacteria, but also directly from the environment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163344/systemic-and-local-cytokine-profile-following-spinal-cord-injury-in-rats-a-multiplex-analysis
#15
Yana O Mukhamedshina, Elvira R Akhmetzyanova, Ekaterina V Martynova, Svetlana F Khaiboullina, Luisa R Galieva, Albert A Rizvanov
Our study of the changes in cytokine profile in blood serum and in the spinal cord after traumatic spinal cord injury (SCI) has shown that an inflammatory reaction and immunological response are not limited to the CNS, but widespread. This fact was confirmed by changes detected in a cytokine profile in blood serum samples [MIP-1α, interleukin 1 (IL-1) α, IL-2, IL-5, IL-1β, MCP-1, RANTES]. There were also changes in the levels of MIP-1α, IL-1α, IL-2, IL-5, IL-18, GM-colony-stimulating factor, IL-17α, IFN-γ, IL-10, IL-13, MCP-1, and GRO KC CINC-1 in samples of the rat injured spinal cord...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/29162808/a-multiplexable-tale-based-binary-expression-system-for-in-vivo-cellular-interaction-studies
#16
Markus Toegel, Ghows Azzam, Eunice Y Lee, David J H F Knapp, Ying Tan, Ming Fa, Tudor A Fulga
Binary expression systems have revolutionised genetic research by enabling delivery of loss-of-function and gain-of-function transgenes with precise spatial-temporal resolution in vivo. However, at present, each existing platform relies on a defined exogenous transcription activator capable of binding a unique recognition sequence. Consequently, none of these technologies alone can be used to simultaneously target different tissues or cell types in the same organism. Here, we report a modular system based on programmable transcription activator-like effector (TALE) proteins, which enables parallel expression of multiple transgenes in spatially distinct tissues in vivo...
November 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/29162042/novel-intragenic-deletions-within-the-ube3a-gene-in-two-unrelated-patients-with-angelman-syndrome-case-report-and-review-of-the-literature
#17
Cinthia Aguilera, Marina Viñas-Jornet, Neus Baena, Elisabeth Gabau, Concepción Fernández, Nuria Capdevila, Sanja Cirkovic, Adrijan Sarajlija, Marijana Miskovic, Danijela Radivojevic, Anna Ruiz, Miriam Guitart
BACKGROUND: Patients with Angelman syndrome (AS) are affected by severe intellectual disability with absence of speech, distinctive dysmorphic craniofacial features, ataxia and a characteristic behavioral phenotype. AS is caused by the lack of expression in neurons of the UBE3A gene, which is located in the 15q11.2-q13 imprinted region. Functional loss of UBE3A is due to 15q11.2-q13 deletion, mutations in the UBE3A gene, paternal uniparental disomy and genomic imprinting defects. CASE PRESENTATION: We report here two patients with clinical features of AS referred to our hospital for clinical follow-up and genetic diagnosis...
November 21, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/29161617/early-pregnancy-immune-biomarkers-in-peripheral-blood-may-predict-preeclampsia
#18
M D Salazar Garcia, Y Mobley, J Henson, M Davies, A Skariah, S Dambaeva, A Gilman-Sachs, K Beaman, C Lampley, J Kwak-Kim
We performed a prospective cohort study in 197 pregnant women. Peripheral blood was collected between 5 and 16 weeks of gestation. Intracellular cytokine analysis and immunophenotype were performed by flow-cytometry. Serum levels of cytokines and chemokines were analyzed by multiplex assay. 86 patients were eligible for the analysis and 10.5% (n=9) developed preeclampsia. Patients with preeclampsia had significantly higher percentage of CD3(+)CD4(+)TNFα(+) T helper (Th) 1 cells (45.4±10.3 vs 37.1±8.5, P=0...
November 2, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29161506/multiplexed-crispr-cas9-genome-editing-and-gene-regulation-using-csy4-in-saccharomyces-cerevisiae
#19
Raphael Ferreira, Christos Skrekas, Jens Nielsen, Florian David
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has greatly accelerated the field of strain engineering. However, insufficient efforts have been made towards developing robust multiplexing tools in Saccharomyces cerevisiae. Here, we exploit the RNA processing capacity of the bacterial endoribonuclease Csy4 from Pseudomonas aeruginosa, to generate multiple gRNAs from a single transcript for genome editing and gene interference applications in S. cerevisiae. In regards to genome editing, we performed a quadruple deletion of FAA1, FAA4, POX1 and TES1 reaching 96% efficiency out of 24 colonies tested...
November 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29161502/polarization-multiplexing-of-fluorescent-emission-using-multi-resonant-plasmonic-antennas
#20
Eva De Leo, Ario Cocina, Preksha Tiwari, Lisa V Poulikakos, Patricia Marqués-Gallego, Boris leFeber, David J Norris, Ferry Prins
Combining the ability to localize electromagnetic fields at the nanoscale with a directional response, plasmonic antennas offer an effective strategy to shape the far-field pattern of coupled emitters. Here, we introduce a family of directional multi-resonant antennas that allows for polarization-resolved spectral identification of fluorescent emission. The geometry consists of a central aperture surrounded by concentric polygonal corrugations. By varying the periodicity of each axis of the polygon individually, this structure can support multiple resonances that provide independent control over emission directionality for multiple wavelengths...
November 21, 2017: ACS Nano
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