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https://www.readbyqxmd.com/read/28431037/gestational-diabetes-alters-functions-in-offspring-s-umbilical-cord-cells-with-implications-for-cardiovascular-health
#1
Ajith Isaac Amrithraj, Anjaneyulu Kodali, Linh Nguyen, Adrian Kee Keong Teo, Cheng Wei Chang, Neerja Karnani, Kai Lyn Ng, Peter D Gluckman, Yap Seng Chong, Walter Stünkel
As non-communicable diseases such as type 2 diabetes mellitus have their roots in prenatal development and conditions such as maternal gestational diabetes (GDM), we aimed to test this hypothesis in primary cells derived from the offspring of GDM mothers compared to control subjects. We have assessed primary umbilical cord derived cells such as human umbilical vein endothelial cells (HUVECs) and Wharton's jelly derived mesenchymal stem cells (WJMSCs) from both, the offspring of GDM and non-GDM mothers. We have compared the primary isolates in cell based assays measuring proliferation, mitochondrial oxygen consumption, and the ability to support blood vessel growth...
April 19, 2017: Endocrinology
https://www.readbyqxmd.com/read/28428057/natural-killer-cells-differentiated-in-vitro-from-cord-blood-cd34-cells-are-more-advantageous-for-use-as-an-immunotherapy-than-peripheral-blood-and-cord-blood-natural-killer-cells
#2
Anna Domogala, Michael Blundell, Adrian Thrasher, Mark W Lowdell, J Alejandro Madrigal, Aurore Saudemont
BACKGROUND AIMS: Natural killer (NK) cells have the potential to become a successful immunotherapy as they can target malignant cells without being direct effectors of graft-versus-host disease. Our group has previously shown that large numbers of functional NK cells can be differentiated in vitro from umbilical cord blood (CB) CD34(+) cells. To produce a clinically relevant and effective immunotherapy, we hypothesized that it is essential that the NK cells are able to proliferate and persist in vivo while maintaining an optimal activation status and killing capacity...
April 17, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28428031/the-effect-of-hypoxia-preconditioning-on-the-neural-and-stemness-genes-expression-profiling-in-human-umbilical-cord-blood-mesenchymal-stem-cells
#3
Maryam Kheirandish, Somaie Piri Gavgani, Shahram Samiee
In recent years, human umbilical cord blood-derived mesenchymal stem cell (hUB-MSCs) has been regarded as an alternative source for stem cell therapy. In this study, we evaluated the effect of hypoxia preconditioning (HPC) on the expression of Nt-3, GFAP, Nestin, Oct-4 and Nanog genes and proliferative capacity of hUB-MSCs in comparison with normoxic conditions. HPC+Hypoxia protocol includes cultured hUB-MSCs for 15min at 2.5% O2 and after that reoxygenation for 30min at 21% O2 (HPC), and then hypoxia preconditioned hUB-MSCs subjected to 2...
March 31, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28426990/concentration-of-perfluorinated-compounds-and-cotinine-in-human-foetal-organs-placenta-and-maternal-plasma
#4
Linn Salto Mamsen, Bo A G Jönsson, Christian H Lindh, Rasmus H Olesen, Agnete Larsen, Erik Ernst, Thomas W Kelsey, Claus Yding Andersen
BACKGROUND: Perfluoroalkyl substances (PFASs) are bio-accumulative pollutants, and prenatal exposure to PFASs is believed to impact human foetal development and may have long-term adverse health effects later in life. Additionally, maternal cigarette smoking may be associated with PFAS levels. Foetal exposure has previously been estimated from umbilical cord plasma, but the actual concentration in foetal organs has never been measured. OBJECTIVES: The concentrations of 5 PFASs and cotinine - the primary metabolite of nicotine - were measured in human foetuses, placentas, and maternal plasma to evaluate to what extent these compounds were transferred from mother to foetus and to determine if the PFAS concentrations were associated with maternal cigarette smoking...
April 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28426650/neural-injury-markers-in-intrauterine-growth-restriction-and-their-relation-to-perinatal-outcomes
#5
E Mazarico, E Llurba, R Cumplido, A Valls, J C Melchor, M Iglesias, L Cabero, E Gratacós, Md Gómez Roig
BACKGROUND: The aims of this study were to: (1) compare the concentrations of two neural injury markers, S100B protein and neuron-specific enolase (NSE), in intrauterine growth-restricted fetuses (IUGR) and in fetuses with appropriate growth-for-gestational-age (AGA), and (2) investigate potential relationships between concentrations of these markers, Doppler abnormalities, and adverse perinatal or neonatal outcomes. METHODS: This was a case-controlled, cooperative, prospective study among Spanish Maternal and Child Health Network (Retic SAMID) hospitals...
April 20, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28424512/human-umbilical-cord-plasma-proteins-revitalize-hippocampal-function-in-aged-mice
#6
Joseph M Castellano, Kira I Mosher, Rachelle J Abbey, Alisha A McBride, Michelle L James, Daniela Berdnik, Jadon C Shen, Bende Zou, Xinmin S Xie, Martha Tingle, Izumi V Hinkson, Martin S Angst, Tony Wyss-Coray
Ageing drives changes in neuronal and cognitive function, the decline of which is a major feature of many neurological disorders. The hippocampus, a brain region subserving roles of spatial and episodic memory and learning, is sensitive to the detrimental effects of ageing at morphological and molecular levels. With advancing age, synapses in various hippocampal subfields exhibit impaired long-term potentiation, an electrophysiological correlate of learning and memory. At the molecular level, immediate early genes are among the synaptic plasticity genes that are both induced by long-term potentiation and downregulated in the aged brain...
April 19, 2017: Nature
https://www.readbyqxmd.com/read/28422936/phenotypic-characterization-of-adherent-cells-population-cd34-cd90-cd105-derived-from-wharton-s-jelly
#7
Irena Walecka, Paulina Gil-Kulik, Arkadiusz Krzyżanowski, Marcin Czop, Dariusz Galkowski, Jolanta Karwat, Piotr Chomik, Małgorzata Świstowska, Anna Kwaśniewska, Anna Bogucka-Kocka, Janusz Kocki
BACKGROUND Mesenchymal stromal cells, MSCs, show expression of specific antigens on their surface. The aim of the study is to assess the phenotype of stem cells like isolated from the umbilical cord with respect to the presence of surface antigens CD34, CD90, and CD105 and differences in the expression of surface antigens in cells isolated from freshly sampled material in comparison with the phenotype of cells from in vitro culture. MATERIAL AND METHODS Stem cells collected from the umbilical cord from healthy patients and then cultured in vitro...
April 19, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28422580/glyphosate-and-paraquat-in-maternal-and-fetal-serums-in-thai-women
#8
Pornpimol Kongtip, Noppanun Nangkongnab, Ratanavadee Phupancharoensuk, Chonlada Palarach, Dusit Sujirarat, Supha Sangprasert, Malasod Sermsuk, Namthip Sawattrakool, Susan Renee Woskie
OBJECTIVES: This longitudinal study measured the glyphosate and paraquat concentrations found in maternal and umbilical cord serum in 82 pregnant women who gave birth in three provinces of Thailand. METHODS: Through questionnaires and biological samples collected at childbirth, factors such as personal characteristics, occupation, agricultural activities, and herbicide use in agricultural work were evaluated as predictors of glyphosate and paraquat levels in the pregnant women...
April 19, 2017: Journal of Agromedicine
https://www.readbyqxmd.com/read/28421440/transcriptomic-changes-in-human-umbilical-cord-blood-endothelial-cells-under-simulated-microgravity
#9
E G Rudimov, E N Knjazev, N A Khaustova, O V Grigorieva, L B Buravkova
Microarray analysis of cultured endothelial cells was performed 24 h after simulated microgravity. A significant change in the expression of 177 genes that can be classified into several functional clusters was detected. Among the genes with overexpression, clusters of cell response to external stimuli and regulation of cell motility and proliferation can be reliably distinguished. Among down-regulated genes, clusters of transcription factors with the "zinc fingers" domain and factors involved in the regulation of morphogenesis and angiogenesis were distinguished...
January 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28421147/electrophysiological-morphological-and-ultrastructural-features-of-the-injured-spinal-cord-tissue-after-transplantation-of-human-umbilical-cord-blood-mononuclear-cells-genetically-modified-with-the-vegf-and-gdnf-genes
#10
Y O Mukhamedshina, Z E Gilazieva, S S Arkhipova, L R Galieva, E E Garanina, A A Shulman, G G Yafarova, Y A Chelyshev, N V Shamsutdinova, A A Rizvanov
In this study, we examined the efficacy of human umbilical cord blood mononuclear cells (hUCB-MCs), genetically modified with the VEGF and GDNF genes using adenoviral vectors, on posttraumatic regeneration after transplantation into the site of spinal cord injury (SCI) in rats. Thirty days after SCI, followed by transplantation of nontransduced hUCB-MCs, we observed an improvement in H (latency period, LP) and M(Amax) waves, compared to the group without therapy after SCI. For genetically modified hUCB-MCs, there was improvement in Amax of M wave and LP of both the M and H waves...
2017: Neural Plasticity
https://www.readbyqxmd.com/read/28421003/acellular-gelatinous-material-of-human-umbilical-cord-enhances-wound-healing-a-candidate-remedy-for-deficient-wound-healing
#11
Nazihah Bakhtyar, Marc G Jeschke, Laurence Mainville, Elaine Herer, Saeid Amini-Nik
Impaired wound healing is a severe clinical challenge and research into finding effective wound healing strategies is underway as there is no ideal treatment. Gelatinous material from the umbilical cord called Wharton's jelly is a valuable source of mesenchymal stem cells which have been shown to aid wound healing. While the cellular component of Wharton's jelly has been the subject of extensive research during the last few years, little is known about the de-cellularized jelly material of the umbilical cord...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28420812/isolation-of-mesenchymal-stromal-stem-cells-from-cryopreserved-umbilical-cord-blood-cells
#12
Sumie Fujii, Yasuo Miura, Masaki Iwasa, Satoshi Yoshioka, Aya Fujishiro, Noriko Sugino, Hitomi Kaneko, Yoko Nakagawa, Hideyo Hirai, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Taira Maekawa
Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10(8)) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells...
April 18, 2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28420514/detection-of-mosaic-15q11-1-q11-2-deletion-encompassing-nbeap1-and%C3%A2-poteb-in-a-fetus-with-diffuse-lymphangiomatosis
#13
Chih-Ping Chen, Kuo-Gon Wang, Hsu-Kuang Huang, Cheng-Ran Peng, Schu-Rern Chern, Peih-Shan Wu, Yen-Ni Chen, Shin-Wen Chen, Chen-Chi Lee, Wayseen Wang
OBJECTIVE: We present cytogenetic and molecular cytogenetic diagnoses of mosaic deletion of chromosome 15q11.1-q11.2 in a fetus with diffuse lymphangiomatosis. CASE REPORT: A 33-year-old woman underwent amniocentesis at 22 weeks of gestation because of fetal diffuse lymphangiomatosis involving left-side chest, abdominal cavity, thigh and vulva, and intrauterine growth restriction. Amniocentesis revealed a karyotype of 46,XX,del(15) (q11.1q11.2)[9]/46,XX[26]. The mother had a karyotype of 46,XX...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28420433/umbilical-cord-derived-mesenchymal-stem-cells-on-scaffolds-facilitate-collagen-degradation-via-upregulation-of-mmp-9-in-rat-uterine-scars
#14
Lu Xu, Lijun Ding, Lei Wang, Yun Cao, Hui Zhu, Jingjie Lu, Xin'an Li, Tianran Song, Yali Hu, Jianwu Dai
BACKGROUND: Severe injuries of the uterus may trigger uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat uterine scars following full-thickness excision of uterine walls...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28419547/perinatal-dna-methylation-at-cdkn2a-is-associated-with-offspring-bone-mass-findings-from-the-southampton-women-s-survey
#15
Elizabeth M Curtis, Robert Murray, Philip Titcombe, Eloïse Cook, Rebecca Clarke-Harris, Paula Costello, Emma Garratt, Joanna D Holbrook, Sheila Barton, Hazel Inskip, Keith M Godfrey, Christopher G Bell, Cyrus Cooper, Karen A Lillycrop, Nicholas C Harvey
Poor intrauterine and childhood growth has been linked with the risk of osteoporosis in later life, a relationship which may in part be mediated through altered epigenetic regulation of genes. We previously identified a region within the promoter of the long non-coding RNA ANRIL encoded by the CDKN2A locus, at which differential DNA methylation at birth showed correlations with offspring adiposity. Given the common lineage of adipocytes and osteoblasts, we investigated the relationship between perinatal CDKN2A methylation and bone mass at ages 4 and 6 years...
April 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28418785/metabolomics-analysis-of-the-osteogenic-differentiation-of-umbilical-cord-blood-mesenchymal-stem-cells-reveals-differential-sensitivity-to-osteogenic-agents
#16
Michail E Klontzas, Spyros I Vernardis, Manolis Heliotis, Eleftherios Tsiridis, Athanasios Mantalaris
Mesenchymal stem cells (MSCs) of fetal origin, such as umbilical cord blood MSCs (UCB MSCs), have emerged as a promising cell source for musculoskeletal tissue regeneration because of their higher proliferation potential, lack of donor site morbidity, and their off-the-shelf potential. MSCs differentiated toward the osteogenic lineage exhibit a specific metabolic phenotype characterized by reliance to oxidative phosphorylation for energy production and reduced glycolytic rates. Currently, limited information exists on the metabolic transitions at different stages of the osteogenic process after osteoinduction with different agents...
April 18, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28417027/decision-to-delivery-time-intervals-in-emergency-caesarean-section-cases-repeated-cross-sectional-study-from-oman
#17
Kaukab Tashfeen, Malini Patel, Ilham M Hamdi, Ibrahim H A Al-Busaidi, Mansour N Al-Yarubi
OBJECTIVES: In cases of fetal intolerance to labour, meeting the standard decision-to-delivery time interval (DDI) of ≤30 minutes is challenging. This study aimed to assess DDIs in emergency Caesarean section (CS) cases to identify factors causing DDI delays and the impact of a delayed DDI on perinatal outcomes. METHODS: This repeated cross-sectional study included all emergency CS procedures performed due to acute fetal distress, antepartum haemorrhage or umbilical cord prolapse at the Nizwa Hospital, Nizwa, Oman...
February 2017: Sultan Qaboos University Medical Journal
https://www.readbyqxmd.com/read/28416409/-recommendations-for-the-unequivocal-identification-of-the-newborn
#18
Ester Sanz López, Manuel Sánchez Luna, Segundo Rite Gracia, Isabel Benavente Fernández, José Luis Leante Castellanos, Alejandro Pérez Muñuzuri, César W Ruiz Campillo, María Dolores Sánchez Redondo
Newborn identification is a legal right recognised by international and national laws. Moreover, improving the accuracy of correct patient identification is an important goal of patient safety solutions programs. In this article, the Standards Committee of the Spanish Society of Neonatology establishes recommendations to ensure correct identification of the newborn whilst in hospital. Currently, the most reliable method of identification of the newborn is the combination of identification cord clamp and bracelets (mother bracelet, newborn bracelet and cord clamp with the same number and identical and exclusive barcode system for each newborn) and the collection of maternal and umbilical cord blood samples (for DNA testing only for identification purposes)...
April 14, 2017: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/28415763/low-numbers-of-pre-leukemic-fusion-genes-are-frequently-present-in-umbilical-cord-blood-without-affecting-dna-damage-response
#19
Pavol Kosik, Milan Skorvaga, Matus Durdik, Lukas Jakl, Ekaterina Nikitina, Eva Markova, Katarina Kozics, Eva Horvathova, Igor Belyaev
Despite widely accepted notion that many childhood leukemias are likely developed from hematopoietic stem/progenitor cells (HSPC) with pre-leukemic fusion genes (PFG) formed in embryonic/fetal development, the data on PFG incidence in newborns are contradictive. To provide a better understanding of a prenatal origin of leukemia, umbilical cord blood from 500 newborns was screened for the presence of the most frequent PFG associated with pediatric B-cell acute lymphoblastic leukemia. This screening revealed relatively high incidence of ETV6-RUNX1, BCR-ABL1 (p190) and MLL-AF4 at very low frequencies, averaging ~14 copies per 100,000 cells...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415626/hematopoietic-stem-progenitor-cells-are-less-prone-to-undergo-apoptosis-than-lymphocytes-despite-similar-dna-damage-response
#20
Matus Durdik, Pavol Kosik, Jana Kruzliakova, Lukas Jakl, Eva Markova, Igor Belyaev
Hematopoietic stem/progenitor CD34+ cells (HSPC) give rise to all types of blood cells and represent a key cellular target for origination of leukemia. Apoptosis and repair of DNA double strand breaks (DSB) are vital processes in leukemogenesis. High doses of ionizing radiation are the best known agent that induces leukemia, but less is known about the leukemogenic potential of low doses. While umbilical cord blood (UCB) serves as a valuable source of the HSPC for both research and clinics, the data on DNA damage response and apoptosis in UCB HSPC are very limited...
March 22, 2017: Oncotarget
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