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https://www.readbyqxmd.com/read/28214833/nogo-b-facilitates-lps-mediated-immune-responses-by-up-regulation-of-tlr4-signaling-in-macrophage-raw264-7
#1
Ying Zhu, Qiang Tong, Jia Ye, Yunye Ning, Ye Xiong, Meng Yang, Hua Xiao, Jian Lu, Wujian Xu, Jiandong Li, Qiang Li
BACKGROUND/AIMS: Nogo-B, a member of the reticulon family of proteins, is mainly located in the endoplasmic reticulum (ER). Here, we investigate the function and mechanism of Nogo-B in the regulation of TLR4-associated immune responses in the macrophage cell line of RAW264.7. METHODS: Nogo-B was up- and down-regulated through the use of appropriate adenoviral vectors or siRNA, and the effects of Nogo-B on macrophages under liposaccharide (LPS) stimulation were evaluated via western blotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), flow cytometric analysis, and transwell assay...
January 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214521/neuroprotective-effect-of-salvianolic-acid-b-against-cerebral-ischemic-injury-in-rats-via-the-cd40-nf-%C3%AE%C2%BAb-pathway-associated-with-suppression-of-platelets-activation-and-neuroinflammation
#2
Shixin Xu, Aiqin Zhong, Huining Ma, Dan Li, Yue Hu, Yingzhi Xu, Junping Zhang
Neuroinflammation plays a critical role in the pathogenesis of ischemia/reperfusion (I/R) injury. Activated platelets are increasingly regarded as initiators and/or amplifiers of inflammatory processes in cerebral I/R injury. Salvianolic acid B (SAB) is the most abundant bioactive compound of Salviae miltiorrhizae, a well-known Chinese herb used to promote blood circulation and eliminating blood stasis. S. miltiorrhizae has been used clinically in Asia for the treatment of ischemic cerebrovascular diseases...
February 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28212413/scarless-deletion-of-up-to-seven-methyl-accepting-chemotaxis-genes-with-an-optimized-method-highlights-key-function-of-chem-in-salmonella-typhimurium
#3
Stefanie Hoffmann, Christiane Schmidt, Steffi Walter, Jennifer K Bender, Roman G Gerlach
Site-directed scarless mutagenesis is an essential tool of modern pathogenesis research. We describe an optimized two-step protocol for genome editing in Salmonella enterica serovar Typhimurium to enable multiple sequential mutagenesis steps in a single strain. The system is based on the λ Red recombinase-catalyzed integration of a selectable antibiotics resistance marker followed by replacement of this cassette. Markerless mutants are selected by expressing the meganuclease I-SceI which induces double-strand breaks in bacteria still harboring the resistance locus...
2017: PloS One
https://www.readbyqxmd.com/read/28212115/predictor-of-hand-radiological-progression-in-patients-with-rheumatoid-arthritis-receiving-tnf-antagonist-therapy-by-change-in-grayscale-synovitis-a-preliminary-study
#4
Ying-Chou Chen, Fu-Mei Su, Shih-Wei Hsu, Jia-Feng Chen, Tien-Tsai Cheng, Han-Ming Lai, Wen-Chan Chiu
OBJECTIVES: This prospective study aimed to compare synovial ultrasound scores to conventional measures (DAS28, CRP levels) in predicting radiographic progression in patients with rheumatoid arthritis under TNF antagonist therapy. METHODS: Patients with RA who received TNF antagonist therapy were enrolled, all of whom underwent clinical, laboratory, and ultrasonographic assessments with grayscale and power Doppler assessments of bilateral elbows (anterior and posterior recess), wrists (dorsal, palmar, and ulnar aspects), second and third MCP joints (dorsal and palmar recess), and PIP II and III (dorsal and palmar) at baseline and at 1, 3 months...
February 17, 2017: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/28209068/urinary-cytokines-chemokines-after-magnetic-resonance-guided-high-intensity-focused-ultrasound-for-palliative-treatment-of-painful-bone-metastases
#5
Ahmad Bushehri, Gregory Czarnota, Liying Zhang, Kullervo Hynynen, Yuexi Huang, Michael Chan, William Chu, Kristopher Dennis, Charles Mougenot, Jennifer Coccagna, Arjun Sahgal, Edward Chow, Carlo DeAngelis
BACKGROUND: Pain is experienced by 50-75% of patients with bone metastases, representing a major source of morbidity amongst cancer patients. Magnetic resonance-guided high intensity focused ultrasound (MRgHIFU) is a new, non-invasive, outpatient treatment modality for painful bone metastases. The aim of this study was to analyze urinary cytokines/chemokines pattern after MRgHIFU for palliative treatment of painful bone metastases. The findings were compared to the cytokines/chemokines pattern post single 8 Gy fraction radiation from our previous study...
January 2017: Annals of Palliative Medicine
https://www.readbyqxmd.com/read/28208647/soy-leaf-extract-exerts-atheroprotective-effects-via-modulation-of-kr%C3%A3-ppel-like-factor-2-and-adhesion-molecules
#6
Jong-Min Han, Hua Li, Moon-Hee Cho, Seung-Hwa Baek, Chul-Ho Lee, Ho-Yong Park, Tae-Sook Jeong
Soy-leaf extracts exert their cardioprotective effects by inducing endothelium-dependent vasodilation in the arteries, and they favorably modulate the serum lipid profile. In this study, we investigated the atheroprotective effects of an ethanol extract of soy leaf (ESL) in human umbilical vein endothelial cells (HUVECs) and high-cholesterol diet (HCD)-fed low-density lipoprotein receptor deficient (LDLR-/-) mice. ESL induced the expression of Krüppel-like factor 2 (KLF2), an endothelial transcription factor, and endothelial nitric oxide synthase (eNOS), and suppressed the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) through moderate inflammatory signal activation, not only in tumor necrosis factor-α (TNF-α)-stimulated HUVECs but also in 7-ketocholesterol (7-KC)-stimulated HUVECs...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28208630/cocoa-and-grape-seed-byproducts-as-a-source-of-antioxidant-and-anti-inflammatory-proanthocyanidins
#7
María De La Luz Cádiz-Gurrea, Isabel Borrás-Linares, Jesús Lozano-Sánchez, Jorge Joven, Salvador Fernández-Arroyo, Antonio Segura-Carretero
Phenolic compounds, which are secondary plant metabolites, are considered an integral part of the human diet. Physiological properties of dietary polyphenols have come to the attention in recent years. Especially, proanthocyanidins (ranging from dimers to decamers) have demonstrated potential interactions with biological systems, such as antiviral, antibacterial, molluscicidal, enzyme-inhibiting, antioxidant, and radical-scavenging properties. Agroindustry produces a considerable amount of phenolic-rich sources, and the ability of polyphenolic structures to interacts with other molecules in living organisms confers their beneficial properties...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28207826/changes-of-plasma-cytokines-and-chemokines-expression-level-in-nasopharyngeal-carcinoma-patients-after-treatment-with-definitive-intensity-modulated-radiotherapy-imrt
#8
Ya-Bin Jin, Guo-Yi Zhang, Kai-Rong Lin, Xiang-Ping Chen, Jin-Huan Cui, Yue-Jian Wang, Wei Luo
BACKGROUND: Potential clinical application values of certain cytokines and chemokines that participate in the process of tumor growth, invasion, and metastasis have been reported. However, there still lack of biomarkers for a great many of malignancy. This study identified cytokines or chemokines involved in the occurrence and development of nasopharyngeal carcinoma (NPC), which might be a biomarker for noninvasive early diagnosis. METHODS: The plasma levels of 19 cytokines and chemokines were detected by the luminex liquid array-based multiplexed immunoassays in 39 NPC patients before and after treatment by definitive intensity-modulated radiotherapy (IMRT)...
2017: PloS One
https://www.readbyqxmd.com/read/28207427/progression-and-characterization-of-the-accelerated-atherosclerosis-in-iliac-artery-of-new-zealand-white-rabbits-effect-of-simvastatin
#9
Jitendra S Kanshana, Vivek Khanna, Vishal Singh, Manish Jain, Ankita Misra, Sachin Kumar, Mariya Farooqui, Manoj K Barthwal, Madhu Dikshit
OBJECTIVE: Although atherosclerosis is described in New Zealand White rabbit's iliac artery, yet details of time-dependent atherosclerosis progression are not well known. Further, a well characterized accelerated model of atherosclerosis is also required for the screening of candidate drugs to target specific steps of atherosclerosis development. The present study extensively characterizes the time-dependent plaque composition and functional responses of the atherosclerosis in rabbit iliac artery and its modification by simvastatin...
February 14, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28205525/testicular-activin-and-follistatin-levels-are-elevated-during-the-course-of-experimental-autoimmune-epididymo-orchitis-in-mice
#10
Nour Nicolas, Vera Michel, Sudhanshu Bhushan, Eva Wahle, Susan Hayward, Helen Ludlow, David M de Kretser, Kate L Loveland, Hans-Christian Schuppe, Andreas Meinhardt, Mark P Hedger, Monika Fijak
Experimental autoimmune epididymo-orchitis (EAEO) is a model of chronic inflammation, induced by immunisation with testicular antigens, which reproduces the pathology of some types of human infertility. Activins A and B regulate spermatogenesis and steroidogenesis, but are also pro-inflammatory, pro-fibrotic cytokines. Expression of the activins and their endogenous antagonists, inhibin and follistatin, was examined in murine EAEO. Adult untreated and adjuvant-treated control mice showed no pathology. All mice immunised with testis antigens developed EAEO by 50 days, characterised by loss of germ cells, immune cell infiltration and fibrosis in the testis, similar to biopsies from human inflamed testis...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28204966/inhibition-of-the-renin-angiotensin-system-post-myocardial-infarction-prevents-inflammation-associated-acute-cardiac-rupture
#11
Xiao-Ming Gao, Alan Tsai, Annas Al-Sharea, Yidan Su, Shirley Moore, Li-Ping Han, Helen Kiriazis, Anthony M Dart, Andrew J Murphy, Xiao-Jun Du
PURPOSE: Inhibition of the renin-angiotensin system (RAS) is beneficial in patient management after myocardial infarction (MI). However, whether RAS inhibition also provides cardiac protection in the acute phase of MI is unclear. METHODS: Male 129sv mice underwent coronary artery occlusion to induce MI, followed by treatment with losartan (L, 20 and 60 mg/kg), perindopril (P, 2 and 6 mg/kg), amlodipine (20 mg/kg as a BP-lowering agent) or vehicle as control. Drug effects on hemodynamics were examined...
February 15, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28202909/leukocyte-tlr5-deficiency-inhibits-atherosclerosis-by-reduced-macrophage-recruitment-and-defective-t-cell-responsiveness
#12
Guilielmus H J M Ellenbroek, Gijs H M van Puijvelde, Adam A Anas, Martine Bot, Miriam Asbach, Arjan Schoneveld, Peter J van Santbrink, Amanda C Foks, Leo Timmers, Pieter A Doevendans, Gerard Pasterkamp, Imo E Hoefer, Tom van der Poll, Johan Kuiper, Saskia C A de Jager
Toll-like receptors (TLR) provide a critical link between innate and adaptive immunity, both important players in atherosclerosis. Since evidence for the role of TLR5 is lacking, we aimed to establish this in the immune axis of atherosclerosis. We assessed the effect of the TLR5-specific ligand Flagellin on macrophage maturation and T-cell polarisation. Next, we generated TLR5(-/-)LDLr(-/-) chimeras to study the effect of hematopoietic TLR5 deficiency on atherosclerosis formation. Flagellin stimulation did not influence wildtype or TLR5(-/-) macrophage maturation...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28202856/plasma-monocyte-chemoattractant-protein-1-as-a-predictive-marker-for-sepsis-prognosis-a-prospective-cohort-study
#13
Tingting Zhu, Xuelian Liao, Ting Feng, Qihong Wu, Jiangqian Zhang, Xing Cao, Hong Li
Sepsis is a systemic host response to infection, and patients with sepsis are frequently handled in the intensive care unit. However, mortality related to sepsis remains high throughout the world. In addition, there have been no efficient prognostic biomarkers for sepsis to be employed in clinical practice. We therefore aimed to identify prognostic biomarkers for sepsis using the chemokine/cytokine array. This study included 143 patients with sepsis, who were divided into survivor and nonsurvivor groups according to their 28-day mortality status...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28202529/aberrant-syk-kinase-signaling-is-essential-for-tumorigenesis-induced-by-tsc2-inactivation
#14
Ye Cui, Wendy K Steagall, Anthony Lamattina, Gustavo Pacheco-Rodriguez, Mario Stylianou, Pranav Kidambi, Benjamin Stump, Fernanda Golzarri, Ivan O Rosas, Carmen Priolo, Elizabeth P Henske, Joel Moss, Souheil El-Chemaly
Somatic or germline mutations in the tuberous sclerosis complex (TSC) tumor suppressor genes are associated closely with the pathogenesis of lymphangioleiomyomatosis (LAM), a rare and progressive neoplastic disease that predominantly affects women in their childbearing years. Serum levels of the lymphangiogeneic growth factor VEGF-D are elevated significantly in LAM. However, there are gaps in knowledge regarding VEGF-D dysregulation and its cellular origin in LAM. Here we show that increased expression and activation of the tyrosine kinase Syk in TSC2-deficient cells and pulmonary nodules from LAM patients contributes to tumor growth...
February 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28202417/triptolide-attenuates-pressure-overload-induced-myocardial-remodeling-in-mice-via-the-inhibition-of-nlrp3-inflammasome-expression
#15
Rujun Li, Kuiying Lu, Yao Wang, Mingxing Chen, Fengyu Zhang, Hui Shen, Deshan Yao, Kaizheng Gong, Zhengang Zhang
Triptolide is the predominant active component of the Chinese herb Tripterygium wilfordii Hook F (TwHF) that has been widely used to treat several chronic inflammatory diseases due to its immunosuppressive, anti-inflammatory, and anti-proliferative properties. In the present study, we elucidated the cardioprotective effects of triptolide against cardiac dysfunction and myocardial remodeling in chronic pressure-overloaded hearts. Furthermore, the potential mechanisms of triptolide were investigated. For this purpose, C57/BL6 mice were anesthetized and subjected to transverse aortic constriction (TAC) or sham operation...
February 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28202393/role-of-a1-and-a2a-adenosine-receptor-agonists-in-adipose-tissue-inflammation-induced-by-obesity-in-mice
#16
Caroline Candida DeOliveira, Cintia Rabelo E Paiva Caria, Erica Martins Ferreira Gotardo, Marcelo Lima Ribeiro, Alessandra Gambero
Adenosine receptors are expressed in adipose tissue and control physiological and pathological events such as lipolysis and inflammation. The aim of this study was to evaluate the activity of N(6)-cyclopentyladenosine (CPA), a potent and selective A1 adenosine receptor agonist; 2-p-(2-carboxyethyl)phenethylamino-5'-N-ethylcarboxyamidoadenosine hydrochloride (CGS-21680), an A2A adenosine receptor agonist; and 5'-N-ethylcarboxamidoadenosine (NECA), a potent non-selective adenosine receptor agonist on adipose tissue inflammatory alterations induced by obesity in mice...
February 13, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28199352/nearest-shrunken-centroids-via-alternative-genewise-shrinkages
#17
Byeong Yeob Choi, Eric Bair, Jae Won Lee
Nearest shrunken centroids (NSC) is a popular classification method for microarray data. NSC calculates centroids for each class and "shrinks" the centroids toward 0 using soft thresholding. Future observations are then assigned to the class with the minimum distance between the observation and the (shrunken) centroid. Under certain conditions the soft shrinkage used by NSC is equivalent to a LASSO penalty. However, this penalty can produce biased estimates when the true coefficients are large. In addition, NSC ignores the fact that multiple measures of the same gene are likely to be related to one another...
2017: PloS One
https://www.readbyqxmd.com/read/28198773/early-post-operative-results-of-percutaneous-needle-fasciotomy-in-451-patients-with-dupuytren-disease
#18
Sanne Molenkamp, Tanneke A M Schouten, Dieuwke C Broekstra, Paul M N Werker, J Daniel Moolenburgh
BACKGROUND: Percutaneous needle fasciotomy (PNF) is a minimally invasive treatment modality for Dupuytren disease (DD). In this study we analyzed the efficacy and complication-rate of PNF using a statistical method that takes the multi-level structure of data, regarding multiple measurements from the same patients, into account. METHODS: The data of 470 treated rays from 451 patients with Dupuytren disease that underwent percutaneous needle fasciotomy (PNF) were analyzed retrospectively...
February 13, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28197827/phloretin-prevents-high-fat-diet-induced-obesity-and-improves-metabolic-homeostasis
#19
Sary Alsanea, Mingming Gao, Dexi Liu
Reactive oxygen species generated as a by-product in metabolism play a central role in the development of obesity and obesity-related metabolic complications. The objective of the current study is to explore the possibility to block obesity and improve metabolic homeostasis via phloretin, a natural antioxidant product from apple tree leaves and Manchurian apricot. Both preventive and therapeutic activities of phloretin were assessed using a high-fat diet-induced obesity mouse model. Phloretin was injected intraperitoneally twice weekly into regular and obese mice fed a high-fat diet...
February 14, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28196866/sglt2-expression-is-increased-in-human-diabetic-nephropathy-sglt2-inhibition-decreases-renal-lipid-accumulation-inflammation-and-the-development-of-nephropathy-in-diabetic-mice
#20
Xiaoxin X Wang, Jonathan Levi, Yuhuan Luo, Komuraiah Myakala, Michal Herman-Edelstein, Liru Qiu, Dong Wang, Yingqiong Peng, Almut Grenz, Scott Lucia, Evgenia Dobrinskikh, Vivette D D'Agati, Hermann Koepsell, Jeffrey B Kopp, Avi Rosenberg, Moshe Levi
There is very limited human renal sodium gradient dependent glucose transporter protein SGLT2 mRNA and protein expression data reported in the literature. Aim 1 of this study was to determine SGLT2 mRNA and protein levels in human and animal models of diabetic nephropathy. We have found that the expression of SGLT2 mRNA and protein is increased in renal biopsies from human subjects with diabetic nephropathy. This is in contrast to db-db mice which had no changes in renal SGLT-2 protein expression. Furthermore, the effect of SGLT2 inhibition on renal lipid content and inflammation is not known...
February 14, 2017: Journal of Biological Chemistry
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