Juan U Mascotte-Cruz, Arturo Vera, Lorenzo Leija, Francisco E Lopez-Salas, Michael Gradzielski, Joachim Koetz, Bismark Gatica-García, C P Rodríguez-Oviedo, Irais E Valenzuela-Arzeta, Lourdes Escobedo, David Reyes-Corona, M E Gutierrez-Castillo, Minerva Maldonado-Berny, Armando J Espadas-Alvarez, Carlos E Orozco-Barrios, Daniel Martinez-Fong
Neurotensin-polyplex nanoparticles provide efficient gene transfection of nigral dopaminergic neurons when intracerebrally injected in preclinical trials of Parkinson's disease because they do not cross the blood-brain barrier (BBB). Therefore, this study aimed to open BBB with focused ultrasound (FUS) on the substantia nigra to attain systemic and intranasal transfections and evaluate its detrimental effect in rats. Systemically injected Evans Blue showed that a two-pulse FUS opened the nigral BBB. Accordingly, 35 μL of neurotensin-polyplex nanoparticles encompassing the green fluorescent protein plasmid (79...
April 2, 2024: Discov Nano