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https://www.readbyqxmd.com/read/28640945/production-of-human-vitronectin-in-nicotiana-benthamiana-using-the-inpact-hyper-expression-platform
#1
Benjamin Dugdale, Maiko Kato, Pradeep Deo, Manuel Plan, Mark Harrison, Robyn Lloyd, Terry Walsh, Robert Harding, James Dale
Human vitronectin (hVN) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. In vitro, hVN is added to serum-free media in order to promote the adhesion of animal cells to tissue culture surfaces and the proliferation of undifferentiated stem cells. Here, we report the production of hVN in Nicotiana benthamiana using the inducible In Plant Activation (INPACT) hyper-expression platform. N. benthamiana plants were transformed with an INPACT expression cassette encoding hVN, and both the Tobacco yellow dwarf virus Rep/RepA activator and Tomato bushy stunt virus p19 gene under the transcriptional control of the ethanol-inducible AlcR:alcA gene switch...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28638474/self-assembly-of-gold-nanoparticles-shows-microenvironment-mediated-dynamic-switching-and-enhanced-brain-tumor-targeting
#2
Qishuai Feng, Yajing Shen, Yingjie Fu, Megan E Muroski, Peng Zhang, Qiaoyue Wang, Chang Xu, Maciej S Lesniak, Gang Li, Yu Cheng
Inorganic nanoparticles with unique physical properties have been explored as nanomedicines for brain tumor treatment. However, the clinical applications of the inorganic formulations are often hindered by the biological barriers and failure to be bioeliminated. The size of the nanoparticle is an essential design parameter which plays a significant role to affect the tumor targeting and biodistribution. Here, we report a feasible approach for the assembly of gold nanoparticles into ~80 nm nanospheres as a drug delivery platform for enhanced retention in brain tumors with the ability to be dynamically switched into the single formulation for excretion...
2017: Theranostics
https://www.readbyqxmd.com/read/28631225/definition-of-the-upper-reference-limit-for-thyroglobulin-antibodies-according-to-the-national-academy-of-clinical-biochemistry-guidelines-comparison-of-eleven-different-automated-methods
#3
F D'Aurizio, P Metus, A Ferrari, B Caruso, R Castello, D Villalta, A Steffan, K Gaspardo, F Pesente, N Bizzaro, E Tonutti, S Valverde, C Cosma, M Plebani, R Tozzoli
PURPOSE: In the last two decades, thyroglobulin autoantibodies (TgAb) measurement has progressively switched from marker of thyroid autoimmunity to test associated with thyroglobulin (Tg) to verify the presence or absence of TgAb interference in the follow-up of patients with differentiated thyroid cancer. Of note, TgAb measurement is cumbersome: despite standardization against the International Reference Preparation MRC 65/93, several studies demonstrated high inter-method variability and wide variation in limits of detection and in reference intervals...
December 2017: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/28625678/rapid-protein-production-from-stable-cho-cell-pools-using-plasmid-vector-and-the-cumate-gene-switch
#4
Adeline Poulain, Sylvie Perret, Félix Malenfant, Alaka Mullick, Bernard Massie, Yves Durocher
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHO(BRI/rcTA)) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO(BRI/rcTA) pools producing high levels of recombinant proteins...
June 15, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28624971/excelautomat-a-tool-for-systematic-processing-of-files-as-applied-to-quantum-chemical-calculations
#5
Jalal Z A Laloo, Nassirah Laloo, Lydia Rhyman, Ponnadurai Ramasami
The processing of the input and output files of quantum chemical calculations often necessitates a spreadsheet as a key component of the workflow. Spreadsheet packages with a built-in programming language editor can automate the steps involved and thus provide a direct link between processing files and the spreadsheet. This helps to reduce user-interventions as well as the need to switch between different programs to carry out each step. The ExcelAutomat tool is the implementation of this method in Microsoft Excel (MS Excel) using the default Visual Basic for Application (VBA) programming language...
June 17, 2017: Journal of Computer-aided Molecular Design
https://www.readbyqxmd.com/read/28621923/capture-and-release-of-trna-by-the-t-loop-receptor-in-the-function-of-the-t-box-riboswitch
#6
Xianyang Fang, Malgorzata Michnicka, Yikan Zhang, Yun Xing Wang, Edward P Nikonowicz
In Gram-positive bacteria, the tRNA-dependent T-box riboswitch system regulates expression of amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. Binding of uncharged tRNA "closes" the switch, allowing transcription read-through. Structure studies of the 100 nt stem I domain reveal tRNA utilizes base pairing and stacking interactions to bind the stem, but little is known structurally about the 180 nt riboswitch core (stem I, stem III, and antiterminator stem) in complex with tRNA and the mechanism of coupling of the intermolecular binding domains crucial to T-box function...
June 16, 2017: Biochemistry
https://www.readbyqxmd.com/read/28621284/comparison-of-platform-switched-and-sloping-shoulder-implants-on-stress-reduction-in-various-bone-densities-finite-element-analysis
#7
Joji Markose, S Suresh, Shruthi Eshwar, K Rekha, Vipin Jain, Supriya Manvi
INTRODUCTION: Comparison of platform switched (PS), sloping shoulder, and regular implants on stress reduction in various bone densities with finite element analysis. MATERIALS AND METHODS: A total of 12 three-dimensional finite element models were built to analyze the stress distribution model. Nobel Biocare 4.3 × 8 mm regular platform replace select implant with matching diameter easy abutment, Nobel Biocare 4.3 × 8 mm replace select implant PS with 3.5 mm diameter easy abutment, Bicon 4 × 8 mm implant with 4 mm diameter sloping shoulder abutments were created virtually in compact bone density using software...
June 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28620044/let-there-be-light-how-to-use-photoswitchable-cross-linker-to-reprogram-proteins
#8
REVIEW
Daniel Hoersch
Azobenzene is a photo-isomerizing molecule whose end-to-end distance changes upon external illumination. When combined with site-specific reactive groups, it can be used as molecular tweezers to remote-control the structure and function of protein targets. The present study gives a brief overview over the rational design strategies that use an azobenzene-based photoswitchable cross-linker to engineer ON/OFF switches into functional proteins or to reprogram proteins for novel functions. The re-engineered proteins may be used as remote controls for cellular pathways, as light-gated drug delivery platforms or as light-powered machinery of synthetic cells and micro-scaled factories...
June 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28618366/eu-3-functionalized-sc-mofs-turn-on-fluorescent-switch-for-ppb-level-biomarker-of-plastic-pollutant-polystyrene-in-serum-and-urine-and-on-site-detection-by-smartphone
#9
Xiao Lian, Tifang Miao, Xiaoyu Xu, Chi Zhang, Bing Yan
The harm of plastic pollutants for human and environment is being paid more and more attention. Polystyrene (PS) and styrene are toxic compounds used in large quantities in the production of fiberglass reinforced polyesters. In this work, a simple method was designed for independent detecting polystyrene and styrene biomarker (phenylglyoxylic acid, PGA) in serum and urine. We prepared Eu3+ functionalized Sc-based metal-organic frameworks as turn-on fluorescent switch for PGA. The distinct enhanced luminescence is observed from the Eu@MOFs with addition of PGA...
June 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28616966/magnetic-resonance-imaging-guided-multi-drug-chemotherapy-and-photothermal-synergistic-therapy-with-ph-and-nir-stimulation-release
#10
Jichun Yang, Yang Chen, Yu-Hao Li, Xue-Bo Yin
The combination of multi-drug chemotherapy and photothermal therapy (PTT) enhances cancer therapeutic efficacy. Herein, we develop a simple and smart pH/NIR dual-stimulus-responsive degradable mesoporous CoFe2O4@PDA@ZIF-8 sandwich nanocomposite. Mesoporous CoFe2O4 core acts as T2-weighted magnetic resonance (MR) imaging probe, PTT agent, and loading platform of hydrophilic doxorubicin (DOX). A polydopamine (PDA) layer is used to avoid the premature leakage of DOX before arriving at tumor site, enhance PTT efficiency, and facilitate the integration of ZIF-8 (a kind of metal-organic frameworks)...
June 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28607900/impaired-vestibular-function-after-cochlear-implantation-in-children-role-of-static-posturography
#11
Satish Nair, Atul Gupta, Ajith Nilakantan, Ruchika Mittal, Ruchi Dahiya, Sachin Saini, Rachana Prasad, Deepika Vajpayee
To identify vestibular dysfunction in children after cochlear implant surgery and to study the utility of static posturography in evaluating vestibular function in children. A prospective study was carried out on 25 children between 2 and 7 years of age with sensorineural hearing loss with no overt vestibular dysfunction. All children underwent static posturography using Synapsis Posturographic System (SPS) software (Version 3.0, REV C) using a static platform with foam. The centre of pressure (COP) shift was recorded as statokinesiogram on the software and the mean vestibular, visual and somesthetic scores were obtained...
June 2017: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/28596531/rf1-attenuation-enables-efficient-non-natural-amino-acid-incorporation-for-production-of-homogeneous-antibody-drug-conjugates
#12
Gang Yin, Heather T Stephenson, Junhao Yang, Xiaofan Li, Stephanie M Armstrong, Tyler H Heibeck, Cuong Tran, Mary Rose Masikat, Sihong Zhou, Ryan L Stafford, Alice Y Yam, John Lee, Alexander R Steiner, Avinash Gill, Kalyani Penta, Sonia Pollitt, Ramesh Baliga, Christopher J Murray, Christopher D Thanos, Leslie M McEvoy, Aaron K Sato, Trevor J Hallam
Amber codon suppression for the insertion of non-natural amino acids (nnAAs) is limited by competition with release factor 1 (RF1). Here we describe the genome engineering of a RF1 mutant strain that enhances suppression efficiency during cell-free protein synthesis, without significantly impacting cell growth during biomass production. Specifically, an out membrane protease (OmpT) cleavage site was engineered into the switch loop of RF1, which enables its conditional inactivation during cell lysis. This facilitates extract production without additional processing steps, resulting in a scaleable extract production process...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28596010/silica-hydride-based-phases-for-small-molecule-separations-using-automated-liquid-chromatography-mass-spectrometry-method-development
#13
Dananjaya K Appulage, Kevin A Schug
Silica hydride, or Type C silica, has been developed as an alternative chromatographic support material for liquid chromatography. There are various bonded phases available with this new support. For four such phases (Cholesterol, Bidentate C18, Diamond Hydride, and Diol), retention and selectivity behavior were investigated using liquid chromatography coupled with triple quadrupole mass spectrometry. A set of small molecules from several chemical classes of interest, and varying in their physicochemical properties, were chromatographed under both reversed-phase and aqueous normal phase modes...
June 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28595727/alternate-injections-coupled-with-flow-gated-capillary-electrophoresis-for-rapid-and-accurate-quantitative-analysis-of-urine-samples
#14
Qingfu Zhu, Qiyang Zhang, Ning Zhang, Maojun Gong
Capillary electrophoresis (CE) is a powerful separation technique with advantages over HPLC in terms of separation efficiency, speed, and cost. However, CE suffers in poor reproducibility in quantitative chemical analysis, which is one of major drawbacks preventing its widespread use in routine analytical laboratories. Here we report a novel strategy to enhance the quantitative capability of flow-gated CE. The platform integrated dual flow branches to respectively supply a sample and its standard additions that were then alternately injected into a single capillary for rapid separations (typically 20-90 s)...
July 25, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28589725/dual-targeting-nanoparticle-stimulates-the-immune-system-to-inhibit-tumor-growth
#15
Alyssa K Kosmides, John-William Sidhom, Andrew Fraser, Catherine A Bessell, Jonathan P Schneck
We describe the development of a nanoparticle platform that overcomes the immunosuppressive tumor microenvironment. These nanoparticles are coated with two different antibodies that simultaneously block the inhibitory checkpoint PD-L1 signal and stimulate T cells via the 4-1BB co-stimulatory pathway. These "immunoswitch" particles significantly delay tumor growth and extend survival in multiple in vivo models of murine melanoma and colon cancer in comparison to the use of soluble antibodies or nanoparticles separately conjugated with the inhibitory and stimulating antibodies...
June 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/28587929/a-shear-enhanced-cnt-assembly-nanosensor-platform-for-ultra-sensitive-and-selective-protein-detection
#16
Diya Li, Ceming Wang, Gongchen Sun, Satyajyoti Senapati, Hsueh-Chia Chang
Detection and quantification of low-concentration proteins in heterogeneous media are generally plagued by two distinct obstacles: lack of sensitivity due to high dissociation equilibrium constant KD and non-specificity due to an abundance of non-targets with similar KD. Herein, we report a nanoscale protein-sensing platform with a non-equilibrium on-off switch that employs dielectrophoretic and hydrodynamic shear forces to overcome these thermodynamic limitations with irreversible kinetics. The detection sensitivity is achieved with complete association of the antibody-antigen-antibody (Ab-Ag-Ab) complex by precisely and rapidly assembling carbon nanotubes (CNT) across two parallel electrodes via sequential DC electrophoresis and AC dielectrophoresis (DEP), and with single-CNT electron tunneling conductance...
May 31, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28584529/htert-immortalized-bone-mesenchymal-stromal-cells-expressing-rat-galanin-via-a-single-tetracycline-inducible-lentivirus-system
#17
Ke An, Hui-Ping Liu, Xiao-Long Zhong, David Y B Deng, Jing-Jun Zhang, Zhi-Heng Liu
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28580044/an-intra-droplet-particle-switch-for-droplet-microfluidics-using-bulk-acoustic-waves
#18
Anna Fornell, Mathias Ohlin, Fabio Garofalo, Johan Nilsson, Maria Tenje
To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28558368/molecular-imaging-of-inducible-vegf-expression-and-tumor-progression-in-a-breast-cancer-model
#19
Lu Liang, Zhiwei Yue, Wei Du, Yang Li, Hongyan Tao, Di Wang, Ran Wang, Ziwei Huang, Ningning He, Xiaoyan Xie, Zhongchao Han, Na Liu, Zongjin Li
BACKGROUND: Tumor derived vascular endothelial growth factor (VEGF) can stimulate proliferation and migration of endothelial cells and recruit endothelial progenitor cells into tumors for vascular formation via a paracrine manner. Now increasing evidence suggests that VEGF also serves as an autocrine factor promoting cell survival and tumor angiogenesis. Real time visualization of VEGF activity in the early stages of tumor formation using molecular imaging will provide unprecedented insight into the biological processes of cancer...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28558187/compound-quantum-dot-perovskite-optical-absorbers-on-graphene-enhancing-short-wave-infrared-photodetection
#20
Alexander A Bessonov, Mark Allen, Yinglin Liu, Surama Malik, Joseph Bottomley, Ashley Rushton, Ivonne Medina-Salazar, Martti Voutilainen, Sami Kallioinen, Alan Colli, Chris Bower, Piers Andrew, Tapani Ryhänen
Colloidal quantum dots (QDs) combined with a graphene charge transducer promise to provide a photoconducting platform with high quantum efficiency and large intrinsic gain, yet compatible with cost-efficient polymer substrates. The response time in these devices is limited, however, and fast switching is only possible by sacrificing the high sensitivity. Furthermore, tuning the QD size toward infrared absorption using conventional organic capping ligands progressively reduces the device performance characteristics...
June 7, 2017: ACS Nano
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