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Platform switching

Michael Payne, Linda Merrick, Tanya Lawson, Gordon Ritchie, Christopher Lowe
Commutability between human cytomegalovirus (CMV) viral load assays (VLA) is poor, despite the development of a WHO CMV International Standard (CMV IS). We evaluated a new CMV VLA, cobas® CMV, as compared to our current laboratory developed CMV VLA (LDT), for clinical use. Both the LDT and cobas® CMV were run in parallel for 109 patient samples. In addition, 104 replicates, over 8 dilutions, of the CMV IS were tested. Conversion factors and correlation between the two assays were calculated. The correlation coefficient between the LDT and cobas® CMV was 0...
April 16, 2018: Journal of Medical Virology
Ying Li, Christos Argyropoulos
We propose a scheme to realize nonlinear coherent perfect absorption (CPA) at the nanoscale using epsilon-near-zero (ENZ) plasmonic waveguides. The general conditions to achieve CPA in a linear ENZ plasmonic waveguide are analyzed and presented. The proposed ENZ waveguides support an effective ENZ response at their cutoff frequency, where the CPA effect occurs under the illumination of two counterpropagating plane waves with equal amplitudes and appropriate phase distributions. In addition, the strong and uniform field enhancement inside the nanochannels of the waveguides at the ENZ resonance can efficiently boost Kerr nonlinearities, resulting in a new all-optical switching intensity-dependent CPA phenomenon that can be tunable with ultrafast speed...
April 15, 2018: Optics Letters
Jay-Marie Mackenzie, Jo Borrill, Emily Hawkins, Bob Fields, Ian Kruger, Ian Noonan, Lisa Marzano
OBJECTIVES: Suicides by train have devastating consequences for families, the rail industry, staff dealing with the aftermath of such incidents and potential witnesses. To reduce suicides and suicide attempts by rail, it is important to learn how safe interventions can be made. However, very little is known about how to identify someone who may be about to make a suicide attempt at a railway location (including underground/subways). The current research employed a novel way of understanding what behaviours might immediately precede a suicide or suicide attempt at these locations...
April 10, 2018: BMJ Open
Weifang Zhang, Yingwu Li, Bo Jin, Feifei Ren, Hongxun Wang, Wei Dai
A Fiber Bragg Grating (FBG) interrogation system with a self-adaption threshold peak detection algorithm is proposed and experimentally demonstrated in this study. This system is composed of a field programmable gate array (FPGA) and advanced RISC machine (ARM) platform, tunable Fabry-Perot (F-P) filter and optical switch. To improve system resolution, the F-P filter was employed. As this filter is non-linear, this causes the shifting of central wavelengths with the deviation compensated by the parts of the circuit...
April 8, 2018: Sensors
Aditya Tulsyan, Christopher Garvin, Cenk Undey
Biopharmaceutical manufacturing comprises of multiple distinct processing steps that require effective and efficient monitoring of many variables simultaneously in real-time. The state-of-the-art real-time multivariate statistical batch process monitoring (BPM) platforms have been in use in recent years to ensure comprehensive monitoring is in place as a complementary tool for continued process verification to detect weak signals. This article addresses a longstanding, industry-wide problem in BPM, referred to as the 'Low-N' problem, wherein a product has a limited production history...
April 6, 2018: Biotechnology and Bioengineering
Kelly A Schats, Emily A Van Vré, Carolien Boeckx, Martine De Bie, Dorien M Schrijvers, Bart Neyns, Ingrid De Meester, Mark M Kockx
CONTEXT: - The benefit of programmed death ligand-1 (PD-L1) immunohistochemistry (IHC) as a method to select patients who may benefit from programmed death receptor-1 (PD-1)/PD-L1 immunotherapies remains uncertain in many tumor indications. OBJECTIVES: - To compare the commercially available, approved PD-L1 IHC assays (22C3, 28-8, SP142, SP263), specifically identifying the changes in staining output created by altering the detection method. DESIGN: - This pilot study investigates the respective PD-L1 kit assay staining patterns and related scoring of tumor cells and immune cells on lung carcinoma and melanoma...
April 2, 2018: Archives of Pathology & Laboratory Medicine
Hao Li, Haw Yang
This work reports the design and implementation of a multi-function optical microscope for time-dependent spectroscopy on single molecules and single nanoparticles. It integrates the now-routine single-object measurements into one standalone platform so that no reconfiguration is needed when switching between different types of sample or spectroscopy modes. The illumination modes include evanescent field through total internal reflection, dark-field illumination, and epi-excitation onto a diffraction-limited spot suitable for confocal detection...
March 28, 2018: Journal of Chemical Physics
Zhanmin Zhou, Bao Zhang, Dapeng Mao
Torque ripples caused by cogging torque, flux harmonics, and current measurement error seriously restrict the application of a permanent magnet synchronous motor (PMSM), which has been paid more and more attention for the use in inertial stabilized platforms. Sliding mode control (SMC), in parallel with the classical proportional integral (PI) controller, has a high advantage to suppress the torque ripples as its invariance to disturbances. However, since the high switching gain tends to cause chattering and it requires derivative of signals which is not readily obtainable without an acceleration signal sensor...
March 29, 2018: Sensors
Neha Chaudhary, Duane R Wesemann
Somatic assembly of T cell receptor and B cell receptor (BCR) genes produces a vast diversity of lymphocyte antigen recognition capacity. The advent of efficient high-throughput sequencing of lymphocyte antigen receptor genes has recently generated unprecedented opportunities for exploration of adaptive immune responses. With these opportunities have come significant challenges in understanding the analysis techniques that most accurately reflect underlying biological phenomena. In this regard, sample preparation and sequence analysis techniques, which have largely been borrowed and adapted from other fields, continue to evolve...
2018: Frontiers in Immunology
Yan Chen, Joshua Millstein, Yao Liu, Gina Y Chen, Xuelian Chen, Andres Stucky, Cunye Qu, Jian-Bing Fan, Xiao Chang, Ava Soleimany, Kai Wang, Jiangjian Zhong, Jie Liu, Frank D Gilliland, Zhongjun Li, Xi Zhang, Jiang F Zhong
With conventional gene expression profiling, information concerning cellular heterogeneity is often lost in the physical mixing and averaging of millions of cells. Single-cell transcriptome analysis has the potential to address these issues. However, there is a need to determine how many cells are needed to draw meaningful conclusions in each single-cell study. Here, we introduce the concept of "digital lysate" for assessing cellular heterogeneity with a phase-switch microfluidic platform, and apply it to construct a molecular map of transcriptome perturbation during the cell cycle...
March 28, 2018: ACS Nano
G O Alrabeah, J C Knowles, H Petridis
The reduced marginal bone loss observed when using the platform-switching concept may be the result of reduced amounts of tribocorrosion products released to the peri-implant tissues. Therefore, the purpose of this study was to compare the tribocorrosion product release from various platform-matched and platform-switched implant-abutment couplings under cyclic loading. Forty-eight titanium implants were coupled with pure titanium, gold alloy, cobalt-chrome alloy, and zirconia abutments forming either platform-switched or platform-matched groups ( n = 6)...
March 1, 2018: Journal of Dental Research
Wenjia Liu, Antony Warden, Jiahui Sun, Guangxia Shen, Xianting Ding
Portable Polymerase Chain Reaction (PCR) devices combined with microfluidic chips or lateral flow stripes have shown great potential in the field of point-of-need testing (PoNT) as they only require a small volume of patient sample and are capable of presenting results in a short time. However, the detection for multiple targets in this field leaves much to be desired. Herein, we introduce a novel PCR platform by integrating a bottom-well microfluidic chip with an infra-red (IR) excited temperature control method and fluorescence co-detection of three PCR products...
March 2018: Biomicrofluidics
Maarten Glibert, Stijn Vervaeke, Wolfgang Jacquet, Kim Vermeersch, Pär-Olov Östman, Hugo De Bruyn
BACKGROUND: It is claimed that bone remodeling is limited when the implant neck has microthreads and an internal abutment connection. However, comparative studies, excluding confounding factors, are scarce. AIM: This RCT aims to assess whether a coronal microthreaded design and an internal abutment connection affects crestal bone loss, up to one year of function. MATERIALS AND METHODS: Twenty-one maxillary edentulous patients were consecutively treated for a bar supported maxillary overdenture on four different implants with respectively: internal connection and microthreads on the implant neck (I MT); internal connection, no microthreads (I NMT), external connection, with microthreads (E MT), and external connection, without microthreads (E NMT)...
March 25, 2018: Clinical Implant Dentistry and related Research
Almudena Cascajero, Alberto Rastrojo, Francisco Díez-Fuertes, Beatriz Hernández-Novoa, Begoña Aguado, Santiago Moreno, José Alcami, Mayte Pérez-Olmeda
In this study we have characterized quasispecies dynamics and the evolution of viral tropism in naive HIV-1-infected patients treated with a short-course of maraviroc monotherapy (NCT01060618) independently of their tropism. We randomly selected 20 patients displaying different basal tropism -10 R5 and 10 DMX4- at recruitment as determined by phenotypic tests (Trofile® ). Evolution of viral quasiespecies at the end of treatment was determined by ultra-deep sequencing of the V3 region using a 454 Life Sciences Platform and geno2pheno (g2p) algorithm for viral tropism prediction...
March 21, 2018: Journal of Virology
Guanghui Wang, Jie Tan, Minghui Tang, Changbin Zhang, Dongying Zhang, Wenbin Ji, Junhao Chen, Ho-Pui Ho, Xuping Zhang
Centrifugal microfluidics or lab-on-a-disc (LOAD) is a promising branch of lab-on-a-chip or microfluidics. Besides effective fluid transportation and inherently available density-based sample separation in centrifugal microfluidics, uniform actuation of flow on the disc makes the platform compact and scalable. However, the natural radially outward centrifugal force in a LOAD system limits its capacity to perform complex fluid manipulation steps. In order to increase the fluid manipulation freedom and integration capacity of the LOAD system, we propose a binary centrifugal microfluidics platform...
March 16, 2018: Lab on a Chip
Yexin Feng, Zhichang Wang, Jing Guo, Ji Chen, En-Ge Wang, Ying Jiang, Xin-Zheng Li
Proton tunneling is an elementary process in the dynamics of hydrogen-bonded systems. Collective tunneling is known to exist for a long time. Atomistic investigations of this mechanism in realistic systems, however, are scarce. Using a combination of ab initio theoretical and high-resolution experimental methods, we investigate the role played by the protons on the chirality switching of a water tetramer on NaCl(001). Our scanning tunneling spectroscopies show that partial deuteration of the H2 O tetramer with only one D2 O leads to a significant suppression of the chirality switching rate at a cryogenic temperature (T), indicating that the chirality switches by tunneling in a concerted manner...
March 14, 2018: Journal of Chemical Physics
Rodrigo J da Graça, Thomaz M C Fabrin, Luciano S Gasques, Sônia M A P Prioli, Juan A Balbuena, Alberto J Prioli, Ricardo M Takemoto
Cophylogenetic studies aim at testing specific hypotheses to understand the nature of coevolving associations between sets of organisms, such as host and parasites. Monogeneans and their hosts provide and interesting platform for these studies due to their high host specificity. In this context, the objective of the present study was to establish whether the relationship between Anacanthorus spp. with their hosts from the upper Paraná River and its tributaries can be explained by means of cospeciation processes...
2018: PloS One
Laura Lago, Luis da Silva, Isabel Martinez-Silva, Benito Rilo
PURPOSE: The aim of this randomized clinical trial with a 5-year follow-up was to assess the differences in radiographic levels of peri-implant bone crest between tissue-level implants restored with platform matching (control group) and bone-level implants restored with platform switching (test group) in the posterior region. MATERIALS AND METHODS: To assess marginal bone level changes, periapical radiographs were taken at the moment of prosthesis delivery (baseline), at 1 year, and at 5 years after the definitive restoration...
March 2018: International Journal of Oral & Maxillofacial Implants
Rebecca P Chen, Daniel Blackstock, Qing Sun, Wilfred Chen
Inspired by the remarkable ability of natural protein switches to sense and respond to a wide range of environmental queues, here we report a strategy to engineer synthetic protein switches by using DNA strand displacement to dynamically organize proteins with highly diverse and complex logic gate architectures. We show that DNA strand displacement can be used to dynamically control the spatial proximity and the corresponding fluorescence resonance energy transfer between two fluorescent proteins. Performing Boolean logic operations enabled the explicit control of protein proximity using multi-input, reversible and amplification architectures...
March 12, 2018: Nature Chemistry
Jakob Vowinckel, Aleksej Zelezniak, Roland Bruderer, Michael Mülleder, Lukas Reiter, Markus Ralser
Quantitative proteomics is key for basic research, but needs improvements to satisfy an increasing demand for large sample series in diagnostics, academia and industry. A switch from nanoflowrate to microflowrate chromatography can improve throughput and reduce costs. However, concerns about undersampling and coverage have so far hampered its broad application. We used a QTOF mass spectrometer of the penultimate generation (TripleTOF5600), converted a nanoLC system into a microflow platform, and adapted a SWATH regime for large sample series by implementing retention time- and batch correction strategies...
March 12, 2018: Scientific Reports
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