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Dehalococcoides

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https://www.readbyqxmd.com/read/28087526/rapid-enrichment-of-dehalococcoides-like-bacteria-by-partial-hydrophobic-separation
#1
Hanna R Temme, Kipp Sande, Tao Yan, Paige J Novak
: Organohalide respiring bacteria can be difficult to enrich and isolate, which can limit research on these important organisms. The goal of this research was to develop a method to rapidly (minutes to days) enrich these organisms from a mixed community. The method presented is based on the hypothesis that organohalide respiring bacteria would be more hydrophobic than other bacteria as they dehalogenate hydrophobic compounds. The developed method tests this hypothesis by separating a portion of putative organohalide respiring bacteria, those phylogenetically related to Dehalococcoides mccartyi, at the interface between a hydrophobic organic solvent and aqueous media...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28075122/acetylene-fuels-tce-reductive-dechlorination-by-defined-dehalococcoides-pelobacter-consortia
#2
Xinwei Mao, Ronald S Oremland, Tong Liu, Sara Gushgari, Abigail Amanda Landers, Shaun M Baesman, Lisa Alvarez-Cohen
Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C2H2 is known to inhibit bacterial dechlorination. In this study, we show that while high C2H2 (1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by Dehalococcoides mccartyi isolates as well as enrichment cultures containing D. mccartyi sp., low C2H2 (0.4 mM) concentrations do not inhibit growth or metabolism of D. mccartyi. Co-cultures of Pelobacter SFB93, a C2H2-fermenting bacterium, with D...
January 11, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28042183/integrated-anaerobic-aerobic-biodegradation-of-multiple-contaminants-including-chlorinated-ethylenes-benzene-toluene-and-dichloromethane
#3
Miho Yoshikawa, Ming Zhang, Koki Toyota
Complete bioremediation of soils containing multiple volatile organic compounds (VOCs) remains a challenge. To explore the possibility of complete bioremediation through integrated anaerobic-aerobic biodegradation, laboratory feasibility tests followed by alternate anaerobic-aerobic and aerobic-anaerobic biodegradation tests were performed. Chlorinated ethylenes, including tetrachloroethylene (PCE), trichloroethylene (TCE), cis-dichloroethylene (cis-DCE), and vinyl chloride (VC), and dichloromethane (DCM) were used for anaerobic biodegradation, whereas benzene, toluene, and DCM were used for aerobic biodegradation tests...
2017: Water, Air, and Soil Pollution
https://www.readbyqxmd.com/read/28034497/vertical-distribution-of-dehalogenating-bacteria-in-mangrove-sediment-and-their-potential-to-remove-polybrominated-diphenyl-ether-contamination
#4
Ying Pan, Juan Chen, Haichao Zhou, Shazia Farzana, Nora F Y Tam
The removal and degradation of polybrominated diphenyl ethers (PBDEs) in sediments are not clear. The vertical distribution of total and dehalogenating bacteria in sediment cores collected from a typical mangrove swamp in South China and their intrinsic degradation potential were investigated. These bacterial groups had the highest abundances in surface sediments (0-5cm). A 5-months microcosm experiment also showed that surface sediments had the highest rate to remove BDE-47 than deeper sediments (5-30cm) under anaerobic condition...
December 26, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28007851/complete-genome-sequence-of-dehalococcoides-mccartyi-strain-wbc-2-capable-of-anaerobic-reductive-dechlorination-of-vinyl-chloride
#5
Olivia Molenda, Shuiquan Tang, Elizabeth A Edwards
Dehalococcoides mccartyi strain WBC-2 dechlorinates carcinogen vinyl chloride to ethene in the West Branch Canal Creek (WBC-2) microbial consortium used for bioaugmentation. We assembled and closed the complete genome sequence of this prokaryote using metagenomic sequencing from an enrichment culture.
December 22, 2016: Genome Announcements
https://www.readbyqxmd.com/read/28002948/survival-of-vinyl-chloride-respiring-dehalococcoides-mccartyi-under-long-term-electron-donor-limitation
#6
Koshlan Mayer-Blackwell, Mohammad F Azizian, Jennifer K Green, Alfred M Spormann, Lewis Semprini
In anoxic groundwater aquifers, the long-term survival of Dehalococcoides mccartyi populations expressing the gene vcrA (or bvcA) encoding reductive vinyl chloride dehalogenases are important for complete dechlorination of tetrachloroethene (PCE) and trichloroethene (TCE) to non-chlorinated ethene. The absence or inactivity of vcrA-containing Dehalococcoides results in the accumulation of the harmful chlorinated intermediates dichloroethene (DCE) and vinyl chloride (VC). While vcrA-containing Dehalococcoides subpopulations depend on synergistic interaction with other organohalide-respiring populations generating their metabolic electron acceptors (DCE and VC), their survival requires successful competition for electron donor within the entire organohalide-respiring microbial community...
December 21, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27909745/dechlorination-of-three-tetrachlorobenzene-isomers-by-contaminated-harbor-sludge-derived-enrichment-cultures-follows-thermodynamically-favorable-reactions
#7
Yue Lu, Javier Ramiro-Garcia, Pieter Vandermeeren, Steffi Herrmann, Danuta Cichocka, Dirk Springael, Siavash Atashgahi, Hauke Smidt
Dechlorination patterns of three tetrachlorobenzene isomers, 1,2,3,4-, 1,2,3,5-, and 1,2,4,5-TeCB, were studied in anoxic microcosms derived from contaminated harbor sludge. The removal of doubly, singly, and un-flanked chlorine atoms was noted in 1,2,3,4- and 1,2,3,5-TeCB fed microcosms, whereas only singly flanked chlorine was removed in 1,2,4,5-TeCB microcosms. The thermodynamically more favorable reactions were selectively followed by the enriched cultures with di- and/or mono-chlorobenzene as the main end products of the reductive dechlorination of all three isomers...
December 1, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27903429/polyhydroxyalkanoate-as-a-slow-release-carbon-source-for-in-situ-bioremediation-of-contaminated-aquifers-from-laboratory-investigation-to-pilot-scale-testing-in-the-field
#8
Lucia Pierro, Bruna Matturro, Simona Rossetti, Marco Sagliaschi, Salvatore Sucato, Eduard Alesi, Ernst Bartsch, Firoozeh Arjmand, Marco Petrangeli Papini
A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs)...
November 27, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27881975/membrane-complexes-of-syntrophomonas-wolfei-involved-in-syntrophic-butyrate-degradation-and-hydrogen-formation
#9
Bryan R Crable, Jessica R Sieber, Xinwei Mao, Lisa Alvarez-Cohen, Robert Gunsalus, Rachel R Ogorzalek Loo, Hong Nguyen, Michael J McInerney
Syntrophic butyrate metabolism involves the thermodynamically unfavorable production of hydrogen and/or formate from the high potential electron donor, butyryl-CoA. Such redox reactions can occur only with energy input by a process called reverse electron transfer. Previous studies have demonstrated that hydrogen production from butyrate requires the presence of a proton gradient, but the biochemical machinery involved has not been clearly elucidated. In this study, the gene and enzyme systems involved in reverse electron transfer by Syntrophomonas wolfei were investigated using proteomic and gene expression approaches...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27856620/genomic-characterization-of-dehalococcoides-mccartyi-strain-11a5-reveals-a-circular-extrachromosomal-genetic-element-and-a-new-tetrachloroethene-reductive-dehalogenase-gene
#10
Siyan Zhao, Chang Ding, Jianzhong He
Dehalococcoides mccartyi exhibits versatile capabilities to respire halogenated compounds under anaerobic conditions. In this study, we report the assembly and annotation of the complete genome of a chloroethene dechlorinating D. mccartyi strain 11a5. Bearing a 1,461,973 base-pair chromosome, strain 11a5 distinguishes itself from other D. mccartyi strains by possessing a 5,940 base-pair circular extrachromosomal genetic element which contains a reductive dehalogenase homolog. The whole genome of strain 11a5 harbors 31 putative reductive dehalogenase genes...
November 16, 2016: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27844293/widespread-distribution-of-dehalococcoides-mccartyi-in-the-houston-ship-channel-and-galveston-bay-texas-sediments-and-the-potential-for-reductive-dechlorination-of-pcdd-f-in-an-estuarine-environment
#11
Anne-Sophie Charlotte Hieke, Robin Brinkmeyer, Kevin M Yeager, Kimberly Schindler, Saijin Zhang, Chen Xu, Patrick Louchouarn, Peter H Santschi
Sediments in the Houston Ship Channel and upper Galveston Bay, Texas, USA, are polluted with polychlorinated dibenzo-p-dioxins/furans (PCDD/F; ≤46,000 ng/kg dry weight (wt.)) with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener, contributing >50 % of the total toxic equivalents (TEQ) at most locations. We measured PCDD/F concentrations in sediments and evaluated the potential for enhanced in situ biodegradation by surveying for Dehalococcoides mccartyi, an obligate organohalide respiring bacterium...
November 14, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27829029/inferring-gene-networks-for-strains-of-dehalococcoides-highlights-conserved-relationships-between-genes-encoding-core-catabolic-and-cell-wall-structural-proteins
#12
Cresten B Mansfeldt, Gretchen W Heavner, Annette R Rowe, Boris Hayete, Bruce W Church, Ruth E Richardson
The interpretation of high-throughput gene expression data for non-model microorganisms remains obscured because of the high fraction of hypothetical genes and the limited number of methods for the robust inference of gene networks. Therefore, to elucidate gene-gene and gene-condition linkages in the bioremediation-important genus Dehalococcoides, we applied a Bayesian inference strategy called Reverse Engineering/Forward Simulation (REFS™) on transcriptomic data collected from two organohalide-respiring communities containing different Dehalococcoides mccartyi strains: the Cornell University mixed community D2 and the commercially available KB-1® bioaugmentation culture...
2016: PloS One
https://www.readbyqxmd.com/read/27817900/impact-of-estuarine-gradients-on-reductive-dechlorination-of-1-2-3-4-tetrachlorodibenzo-p-dioxin-in-river-sediment-enrichment-cultures
#13
Hang T Dam, Max M Häggblom
Polychlorinated dibenzo-p-dioxins (PCDDs) are among the most persistent organic pollutants. Although the total input of PCDDs into the environment has decreased substantially over the past four decades, their input via non-point sources is still increasing, especially in estuarine metropolitan areas. Here we report on the microbially mediated reductive dechlorination of PCDDs in anaerobic enrichment cultures established from sediments collected from five locations along the Hackensack River, NJ and investigate the impacts of sediment physicochemical characteristics on dechlorination activity...
February 2017: Chemosphere
https://www.readbyqxmd.com/read/27809491/1-2-dichloroethane-exposure-alters-the-population-structure-metabolism-and-kinetics-of-a-trichloroethene-dechlorinating-dehalococcoides-mccartyi-consortium
#14
Koshlan Mayer-Blackwell, Maeva Fincker, Olivia Molenda, Benjamin Callahan, Holly Sewell, Susan Holmes, Elizabeth A Edwards, Alfred M Spormann
Bioremediation of groundwater contaminated with chlorinated aliphatic hydrocarbons such as perchloroethene and trichloroethene can result in the accumulation of the undesirable intermediate vinyl chloride. Such accumulation can either be due to the absence of specific vinyl chloride respiring Dehalococcoides mccartyi or to the inhibition of such strains by the metabolism of other microorganisms. The fitness of vinyl chloride respiring Dehalococcoides mccartyi subpopulations is particularly uncertain in the presence of chloroethene/chloroethane cocontaminant mixtures, which are commonly found in contaminated groundwater...
November 15, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27786438/microbial-catalyzed-reductive-dechlorination-of-polychlorinated-biphenyls-in-hudson-and-grasse-river-sediment-microcosms-determination-of-dechlorination-preferences-and-identification-of-rare-ortho-removal-pathways
#15
Yan Xu, Kelvin B Gregory, Jeanne M VanBriesen
Biodegradation of polychlorinated biphenyls (PCBs) is an important transformation and detoxification route in the environment. To better understand the influence of PCB congener compositions on dechlorination, sediments from two rivers, Hudson and Grasse, and two PCB mixtures (PCB 5/12, 64/71, 105/114, and 149/153/170 in Mixture 1 and PCB 5/12, 64/71, 82/97/99, and 144/170 in Mixture 2) were used for this microcosm study. The Grasse River sediment microcosms exhibited more extensive dechlorination than the Hudson River sediment microcosms...
December 6, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27720723/direct-loop-mediated-isothermal-amplification-on-filters-for-quantification-of-dehalobacter-in-groundwater
#16
Robert D Stedtfeld, Tiffany M Stedtfeld, Farag Samhan, Yogendra H Kanitkar, Paul B Hatzinger, Alison M Cupples, Syed A Hashsham
Nucleic acid amplification of biomarkers is increasingly used to monitor microbial activity and assess remedial performance in contaminated aquifers. Previous studies described the use of filtration, elution, and direct isothermal amplification (i.e. no DNA extraction and purification) as a field-able means to quantify Dehalococcoides spp. in groundwater. This study expands previous work with direct loop mediated isothermal amplification (LAMP) for the detection and quantification of Dehalobacter spp. in groundwater...
October 5, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27708637/microbiome-dynamics-of-a-polychlorobiphenyl-pcb-historically-contaminated-marine-sediment-under-conditions-promoting-reductive-dechlorination
#17
Bruna Matturro, Carla Ubaldi, Simona Rossetti
The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination (RD) process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB-spiked sediments, the knowledge on the identity and metabolic potential of PCB-dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest (SIN) in Italy (Mar Piccolo, Taranto) under conditions promoting the PCBs RD...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27631786/geochemical-and-microbial-community-determinants-of-reductive-dechlorination-at-a-site-biostimulated-with-glycerol
#18
Siavash Atashgahi, Yue Lu, Ying Zheng, Edoardo Saccenti, Maria Suarez-Diez, Javier Ramiro-Garcia, Heinrich Eisenmann, Martin Elsner, Alfons J M Stams, Dirk Springael, Winnie Dejonghe, Hauke Smidt
Biostimulation is widely used to enhance reductive dechlorination of chlorinated ethenes in contaminated aquifers. However, the knowledge on corresponding biogeochemical responses is limited. In this study, glycerol was injected in an aquifer contaminated with cis-dichloroethene (cDCE), and geochemical and microbial shifts were followed for 265 days. Consistent with anoxic conditions and sulfate reduction after biostimulation, MiSeq 16S rRNA gene sequencing revealed temporarily increased relative abundance of Firmicutes, Bacteriodetes and sulfate reducing Deltaproteobacteria...
October 6, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27621279/the-marr-type-regulator-rdh2r-regulates-rdh-gene-transcription-in-dehalococcoides-mccartyi-strain-cbdb1
#19
Lydia Krasper, Hauke Lilie, Anja Kublik, Lorenz Adrian, Ralph Golbik, Ute Lechner
: Reductive dehalogenases are essential enzymes in organohalide respiration and consist of a catalytic subunit A and a membrane protein B, encoded by rdhAB genes. Thirty-two rdhAB genes exist in the genome of Dehalococcoides mccartyi strain CBDB1. To gain a first insight into the regulation of rdh operons, the control of gene expression of two rdhAB genes (cbdbA1453/cbdbA1452 and cbdbA1455/cbdbA1454) by the MarR-type regulator Rdh2R (cbdbA1456) encoded directly upstream was studied using heterologous expression and in vitro studies...
December 1, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27522174/first-evidence-on-the-occurrence-and-dynamics-of-dehalococcoides-mccartyi-pcb-dechlorinase-genes-in-marine-sediment-during-aroclor1254-reductive-dechlorination
#20
B Matturro, M Di Lenola, C Ubaldi, S Rossetti
The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture...
November 15, 2016: Marine Pollution Bulletin
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