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sulfate reducing bacteria

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https://www.readbyqxmd.com/read/29042504/unintended-laboratory-driven-evolution-reveals-genetic-requirements-for-biofilm-formation-by-desulfovibrio-vulgaris-hildenborough
#1
Kara B De León, Grant M Zane, Valentine V Trotter, Gregory P Krantz, Adam P Arkin, Gareth P Butland, Peter J Walian, Matthew W Fields, Judy D Wall
Biofilms of sulfate-reducing bacteria (SRB) are of particular interest as members of this group are culprits in corrosion of industrial metal and concrete pipelines as well as being key players in subsurface metal cycling. Yet the mechanism of biofilm formation by these bacteria has not been determined. Here we show that two supposedly identical wild-type cultures of the SRB Desulfovibrio vulgaris Hildenborough maintained in different laboratories have diverged in biofilm formation. From genome resequencing and subsequent mutant analyses, we discovered that a single nucleotide change within DVU1017, the ABC transporter of a type I secretion system (T1SS), was sufficient to eliminate biofilm formation in D...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/29040506/high-throughput-sequencing-revealed-novel-dehalococcoidia-in-dechlorinating-microbial-enrichments-from-pcb-contaminated-marine-sediments
#2
B Matturro, E Frascadore, S Rossetti
In this study six PCE-to-ethene dechlorinating cultures, fed with a fermentable substrate (lactate) or hydrogen as electron donor, were obtained from PCB and PCE dechlorinating microcosms constructed with PCB contaminated marine sediments.A novel Chloroflexi member (OTU-DIS1) affiliated to Dehalococcoidales Incertae Sedis, only distantly related to known dechlorinating bacteria, dominated the enrichment cultures (up to 86% of total OTUs). Sulfate-, thiosulfate- and sulfur-reducing bacteria affiliated to genera Desulfobacter, Dethiosulfatibacter and Desulfuromusa were also found to lesser extent...
October 11, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29039304/desulfonatronum-parangueonense-sp-nov-a-sulfate-reducing-bacterium-isolated-from-sediment-of-an-alkaline-crater-lake
#3
Maria Fernanda Pérez Bernal, Elcia Margareth Souza Brito, Manon Bartoli, Johanne Aubé, Marie-Laure Fardeau, German Cuevas Rodriguez, Bernard Ollivier, Rémy Guyoneaud, Agnès Hirschler-Réa
Novel Gram-stain-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacteria, designated strains PAR180(T) and PAR190, were isolated from sediments collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR180(T) grew at temperatures between 15 and 40 °C (optimum 35 °C), and at pH between 8.3 and 10.4 (optimum 9). It was halotolerant, growing with up to 8 % (w/v) NaCl. Lactate, formate, pyruvate and ethanol were used as electron donors in the presence of sulfate and were incompletely oxidized to acetate and CO2...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29022513/discrepancies-in-animal-models-of-preterm-birth
#4
Clarence R Manuel, Charles R Ashby, Sandra E Reznik
BACKGROUND: Preterm birth (PTB) is a multifactorial syndrome occurring before the 37th week of full-term pregnancy [1]. Babies delivered preterm experience short-term and long-term complications affecting multiple organ systems, and serious maternal complications include hemorrhage and infection. Each year, an estimated 15 million babies are born preterm, and complications from prematurity are the leading cause of death among children up to 5 years of age [2]. With another increase in PTB rates over the last several years, the United States continues to have the highest incidence of any industrialized country [3]...
October 12, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29018418/microbial-diversity-in-a-hypersaline-sulfate-lake-a-terrestrial-analog-of-ancient-mars
#5
Alexandra Pontefract, Ting F Zhu, Virginia K Walker, Holli Hepburn, Clarissa Lui, Maria T Zuber, Gary Ruvkun, Christopher E Carr
Life can persist under severe osmotic stress and low water activity in hypersaline environments. On Mars, evidence for the past presence of saline bodies of water is prevalent and resulted in the widespread deposition of sulfate and chloride salts. Here we investigate Spotted Lake (British Columbia, Canada), a hypersaline lake with extreme (>3 M) levels of sulfate salts as an exemplar of the conditions thought to be associated with ancient Mars. We provide the first characterization of microbial structure in Spotted Lake sediments through metagenomic sequencing, and report a bacteria-dominated community with abundant Proteobacteria, Firmicutes, and Bacteroidetes, as well as diverse extremophiles...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28990146/a-preliminary-study-to-design-a-floating-treatment-wetland-for-remediating-acid-mine-drainage-impacted-water-using-vetiver-grass-chrysopogon-zizanioides
#6
Jeffrey D Kiiskila, Dibyendu Sarkar, Kailey A Feuerstein, Rupali Datta
Acid mine drainage (AMD) is extremely acidic, sulfate-rich effluent from abandoned or active mine sites that also contain elevated levels of heavy metals. Untreated AMD can contaminate surface and groundwater and pose severe ecological risk. Both active and passive methods have been developed for AMD treatment consisting of abiotic and biological techniques. Abiotic techniques are expensive and can create large amounts of secondary wastes. Passive biological treatment mainly consists of aerobic or anaerobic constructed wetlands...
October 8, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28987647/serpin-9-and-13-regulate-hemolymph-proteases-during-immune-responses-of-manduca-sexta
#7
Yan He, Yang Wang, Picheng Zhao, Subrahmanyam Rayaprolu, Xiuhong Wang, Xiaolong Cao, Haobo Jiang
Serpins are a superfamily of proteins, most of which inhibit cognate serine proteases by forming inactive acyl-enzyme complexes. In the tobacco hornworm Manduca sexta, serpin-1, -3 through -7 negatively regulate a hemolymph serine protease system that activates precursors of the serine protease homologs (SPHs), phenoloxidases (POs), Spätzles, and other cytokines. Here we report the cloning and characterization of M. sexta serpin-9 and -13. Serpin-9, a 402-residue protein most similar to Drosophila Spn77Ba, has R(366) at the P1 position right before the cleavage site; Serpin-13, a 444-residue ortholog of Drosophila Spn28Dc, is longer than the other seven serpins and has R(410) as the P1 residue...
October 5, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28986518/oxic-anoxic-regime-shifts-mediated-by-feedbacks-between-biogeochemical-processes-and-microbial-community-dynamics
#8
Timothy Bush, Muhe Diao, Rosalind J Allen, Ruben Sinnige, Gerard Muyzer, Jef Huisman
Although regime shifts are known from various ecosystems, the involvement of microbial communities is poorly understood. Here we show that gradual environmental changes induced by, for example, eutrophication or global warming can induce major oxic-anoxic regime shifts. We first investigate a mathematical model describing interactions between microbial communities and biogeochemical oxidation-reduction reactions. In response to gradual changes in oxygen influx, this model abruptly transitions between an oxic state dominated by cyanobacteria and an anoxic state with sulfate-reducing bacteria and phototrophic sulfur bacteria...
October 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28984846/bacteria-alone-establish-the-chemical-basis-of-the-wood-fall-chemosynthetic-ecosystem-in-the-deep-sea
#9
Dimitri Kalenitchenko, Nadine Le Bris, Laetitia Dadaglio, Erwan Peru, Arnaud Besserer, Pierre E Galand
Wood-fall ecosystems host chemosynthetic bacteria that use hydrogen sulfide as an electron donor. The production of hydrogen sulfide from decaying wood in the deep-sea has long been suspected to rely on the activity of wood-boring bivalves, Xylophaga spp. However, recent mesocosm experiments have shown hydrogen sulfide production in the absence of wood borers. Here, we combined in situ chemical measurements, amplicon sequencing and metagenomics to test whether the presence of Xylophaga spp.-affected hydrogen sulfide production and wood microbial community assemblages...
October 6, 2017: ISME Journal
https://www.readbyqxmd.com/read/28977605/hydrogen-sulfide-and-sulfate-prebiotic-stimulates-the-secretion-of-glp-1-and-improves-glycemia-in-male-mice
#10
Jennifer Pichette, Nancy Fynn-Sackey, Jeffrey Gagnon
Recently, the gastrointestinal microbiome, and its metabolites, has emerged as a potential regulator of host metabolism. However, to date little is known on the precise mechanisms of how this regulation occurs. Hydrogen sulfide (H2S) is abundantly produced in the colon by sulfate-reducing bacteria (SRB). H2S is a bioactive gas that plays regulatory roles in many systems, including metabolic hormone regulation. This gas metabolite is produced in close proximity to the glucagonlike peptide-1 (GLP-1)-secreting cells in the gut epithelium...
October 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28971978/activation-of-the-sweet-taste-receptor-t1r3-by-the-artificial-sweetener-sucralose-regulates-the-pulmonary-endothelium
#11
Elizabeth O Harrington, Alexander Vang, Julie Braza, Aparna Shil, Havovi Chichger
A hallmark of acute respiratory distress syndrome (ARDS) is pulmonary vascular permeability. In these settings, loss of barrier integrity is mediated by cell-contact disassembly and actin-remodelling. Studies into molecular mechanisms responsible for improving microvascular barrier function are therefore vital in the development of therapeutic targets for reducing vascular permeability in ARDS. The sweet taste receptor, T1R3 is a GPCR, activated following exposure to sweet molecules, to trigger a gustducin-dependent signal cascade...
September 28, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28964812/isolation-and-characterization-of-a-bacteriophage-and-its-utilization-against-multi-drug-resistant-pseudomonas-aeruginosa-2995
#12
Muhsin Jamal, Saadia Andleeb, Fazal Jalil, Muhammad Imran, Muhammad Asif Nawaz, Tahir Hussain, Muhammad Ali, Chythanya Rajanna Das
AIMS: To identify, isolate, and characterize a lytic bacteriophage against the multiple-drug resistant clinical strain of Pseudomonas aeruginosa-2995 and to determine the phage efficacy against the bacterial planktonic cells and the biofilm. MAIN METHODS: Wastewater was used to isolate a bacteriophage. The phage was characterized with Transmission electron microscopy (TEM). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) was used to identify the expressed proteins...
September 27, 2017: Life Sciences
https://www.readbyqxmd.com/read/28955315/analysis-of-bacterial-community-composition-of-corroded-steel-immersed-in-sanya-and-xiamen-seawaters-in-china-via-method-of-illumina-miseq-sequencing
#13
Xiaohong Li, Jizhou Duan, Hui Xiao, Yongqian Li, Haixia Liu, Fang Guan, Xiaofan Zhai
Metal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms that form on metals are complicated, and have not been well studied. This is why we sought to analyze the composition of bacterial communities living on steel structures, together with the influence of ecological factors on these communities...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28955126/intermittent-fasting-prompted-recovery-from-dextran-sulfate-sodium-induced-colitis-in-mice
#14
Toshihiko Okada, Takeshi Otsubo, Teruki Hagiwara, Fumika Inazuka, Eiko Kobayashi, Shinji Fukuda, Takuya Inoue, Kazuhide Higuchi, Yuki I Kawamura, Taeko Dohi
Fasting-refeeding in mice induces transient hyperproliferation of colonic epithelial cells, which is dependent on the lactate produced as a metabolite of commensal bacteria. We attempted to manipulate colonic epithelial cell turnover with intermittent fasting to prompt recovery from acute colitis. Acute colitis was induced in C57BL/6 mice by administration of dextran sulfate sodium in the drinking water for 5 days. From day 6, mice were fasted for 36 h and refed normal bait, glucose powder, or lactylated high-amylose starch...
September 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/28951230/biosensor-for-screening-bacterial-mercury-methylation-example-within-the-desulfobulbaceae
#15
Yannick Colin, Jérôme Gury, Mathilde Monperrus, Sophie Gentes, Paola Ayala, Marisol Goni-Urriza, Rémy Guyoneaud
Mercury methylation and demethylation processes govern the fate of methylmercury in aquatic ecosystems. Under anoxic conditions, methylation activity is mainly of biological origin and is often the result of sulfate-reducing bacteria. In this study, use of a luminescent biosensor for screening methylmercury production was validated by exposing the reporter strain to methylating or non-methylating Desulfovibrio strains. The sensitivity of the biosensor to methylmercury was shown to depend on sulfate-reducing bacterial growth conditions...
September 23, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28934653/mixed-sulfate-reducing-bacteria-enriched-microbial-fuel-cells-for-the-treatment-of-wastewater-containing-copper
#16
Waheed Miran, Jiseon Jang, Mohsin Nawaz, Asif Shahzad, Sang Eun Jeong, Che Ok Jeon, Dae Sung Lee
Microbial fuel cells (MFCs) have been widely investigated for organic-based waste/substrate conversion to electricity. However, toxic compounds such as heavy metals are ubiquitous in organic waste and wastewater. In this work, a sulfate reducing bacteria (SRB)-enriched anode is used to study the impact of Cu(2+) on MFC performance. This study demonstrates that MFC performance is slightly enhanced at concentrations of up to 20 mg/L of Cu(2+), owing to the stimulating effect of metals on biological reactions...
September 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28930182/microbial-community-structure-and-functions-in-ethanol-fed-sulfate-removal-bioreactors-for-treatment-of-mine-water
#17
Malin Bomberg, Jarno Mäkinen, Marja Salo, Mona Arnold
Sulfate-rich mine water must be treated before it is released into natural water bodies. We tested ethanol as substrate in bioreactors designed for biological sulfate removal from mine water containing up to 9 g L(-1) sulfate, using granular sludge from an industrial waste water treatment plant as inoculum. The pH, redox potential, and sulfate and sulfide concentrations were measured twice a week over a maximum of 171 days. The microbial communities in the bioreactors were characterized by qPCR and high throughput amplicon sequencing...
September 20, 2017: Microorganisms
https://www.readbyqxmd.com/read/28929287/performance-evaluation-and-microbial-community-analysis-of-the-function-and-fate-of-ammonia-in-a-sulfate-reducing-egsb-reactor
#18
Depeng Wang, Bo Liu, Xinchun Ding, Xinbo Sun, Zi Liang, Shixiong Sheng, Lingfeng Du
Ammonia is widely distributed in sulfate-reducing bioreactor dealing with sulfate wastewater, which shows potential effect on the metabolic pathway of sulfate and ammonia. This study investigates the sulfate-reducing efficiency and microbial community composition in the sulfate-reducing EGSB reactor with the increasing ammonia loading. Results indicated that, compared with low ammonia loading (166-666 mg/L), the sulfate and organic matter removal efficiencies were improved gradually with the appropriate ammonia loading (1000-2000 mg/L), which increased from 63...
September 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28925553/dispersal-and-inhibitory-roles-of-mannose-2-deoxy-d-glucose-and-n-acetylgalactosaminidase-on-the-biofilm-of-desulfovibrio-vulgaris
#19
Venkata G Poosarla, Thammajun L Wood, Lei Zhu, Daniel S Miller, Bei Yin, Thomas K Wood
Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion. The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein. In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose, and N-acetylgalactosamine (GalNAc), with mannose predominating. Based on these results, we found that the addition of mannose and the non-metabolizable mannose analog 2-deoxy-D-glucose inhibits the biofilm formation of D...
September 19, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28918434/improved-dna-purification-with-quality-assurance-for-evaluation-of-the-microbial-genetic-content-of-constructed-wetlands
#20
Wenda Huang, Jingjing Guo, Ran Tao, Ying Man, Yunv Dai, Yang Yang
Efficient isolation of target DNA is a crucial first step of DNA-based metagenomic analyses of environmental samples. Insufficient quantity and purity of DNA isolated using commercial kits result in missing genetic information, especially for large-diameter substrates in constructed wetlands (CWs). Here, we addressed this problem by devising a cost-effective calcium chloride lysozyme-sodium dodecyl sulfate (SDS) method (CCLS), with key improvements in the steps of humic acid removal and cell lysis. The buffer comprising Tris, EDTA, Na2O2P7 and PVPP (TENP), and skim milk, could reduce adsorption between microorganisms and substrates, and calcium chloride precipitated and removed over 94% of humic acid...
September 16, 2017: Applied Microbiology and Biotechnology
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