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https://www.readbyqxmd.com/read/29656514/microbial-diversity-and-biomarker-analysis-of-modern-freshwater-microbialites-from-laguna-bacalar-mexico
#1
D B Johnson, P A Beddows, T M Flynn, M R Osburn
Laguna Bacalar is a sulfate-rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. While cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three-pronged geobiological approach of microscopy, high-throughput DNA sequencing, and lipid biomarker analyses...
April 15, 2018: Geobiology
https://www.readbyqxmd.com/read/29655682/pristimerin-a-naturally-occurring-triterpenoid-attenuates-tumorigenesis-in-experimental-colitis-associated-colon-cancer
#2
Ju-Hyung Park, Jin-Kyung Kim
BACKGROUND: Pristimerin is a quinonemethide triterpenoid with anti-cancer, anti-angiogenic, anti-inflammatory and anti-protozoal activity. However, the therapeutic role of pristimerin in colitis-associated colorectal carcinogenesis is unknown. PURPOSE: We sought to examine the therapeutic effects of pristimerin on colitis-associated colon cancer induced in mice using azoxymethane (AOM)/dextran sulfate sodium (DSS). The goal was to identify the potential mechanism of action underlying the pharmacological activity of pristimerin...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29653357/controlling-bromate-formation-in-the-co-ii-peroxymonosulfate-process-by-ammonia-chlorine-ammonia-and-ammonia-chlorine-pretreatment-strategies
#3
Li Ling, Zhaobing Li, Jingyun Fang, Chii Shang
The Co(II)/peroxymonosulfate (Co(II)/PMS) process, producing sulfate radicals (SO4 •- ), effectively removes organic pollutants in water, while producing a significant amount of bromate (BrO3 - ) in the presence of bromide (Br- ). This paper investigates the ammonia (NH3 ) addition, chlorine-ammonia (Cl2 -NH3 ) and ammonia-chlorine (NH3 -Cl2 ) pretreatment strategies in controlling BrO3 - formation in 20 min in the Co(II)/PMS process at pH 4.0. The addition of NH3 retarded the BrO3 - formation, but only at a reduction level of about 9...
April 6, 2018: Water Research
https://www.readbyqxmd.com/read/29653114/adrenal-androgen-measurement-for-assessing-the-selectivity-of-adrenal-venous-sampling-in-primary-aldosteronism
#4
Hongyun Li, Xuebin Zhang, Shanmei Shen, Yan Zhang, Wenjing Zhang, Wenhuan Feng, Ping Li, Dalong Zhu
Adrenal venous sampling (AVS) is the reference standard for primary aldosteronism (PA) subtyping. Cortisol is widely used to assess the success of selective AVS, but it is not always reliable. The aim of the present study was to investigate the usefulness of adrenal androgens, compared to cortisol, in assessing the selectivity of AVS. We consecutively recruited 37 patients with PA undergoing AVS. AVS procedures were performed with the bilateral simultaneous technique without cosyntropin stimulation. We collected two baseline blood samples from 10 patients with a 15-min interval between time -15 (t-15) and time 0 (t0) to measure the variability in the levels of the tested hormones over time...
April 10, 2018: Steroids
https://www.readbyqxmd.com/read/29648444/headspace-sampling-paper-based-analytical-device-for-colorimetric-sers-dual-sensing-of-sulfur-dioxide-in-wine
#5
Dan Li, Huazhen Duan, Yadan Ma, Wei Deng
This study demonstrates a novel strategy for colorimetric/surface enhanced Raman scattering (SERS) dual-mode sensing of sulfur dioxide (SO2) by coupling headspace sampling (HS) with paper-based analytical device (PAD). The smart and multifunctional PAD is fabricated with a vacuum filtration method in which 4-mercaptopyridine (Mpy)-modified gold nanorods (GNRs)-reduced graphene oxide (rGO) hybrids (rGO/MPy-GNRs), anhydrous methanol and starch-iodine complex are immobilized into cellulose-based filter papers...
April 12, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29629342/comparison-of-preoperative-infusion-of-magnesium-sulfate-and-mannitol-on-intraocular-pressure-changes-in-patients-undergoing-phacoemulsification-surgery
#6
Kamran Montazeri, Hasanali Soltani, Mojtaba Rahimi Varposhti, Leila Mohajeri
Background: The present study has been designed to compare the effect of magnesium sulfate with mannitol on reducing intraocular pressure (IOP). Materials and Methods: During the phacoemulsification surgery, 105 patients randomly divided into three groups receiving 20% mannitol at a dose of 0.3 g/kg, 50% magnesium sulfate at a dose of 20 mg/kg, and placebo (normal saline), with the same volume (100 ml) and infusion time (10 min), were used for the first, second, and third groups, respectively...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29629026/effect-of-vitamin-c-on-azoxymethane-aom-dextran-sulfate-sodium-dss-induced-colitis-associated-early-colon-cancer-in-mice
#7
Hee-Jin Jeon, Yiseul Yeom, Yoo-Sun Kim, Eunju Kim, Jae-Ho Shin, Pu Reum Seok, Moon Jea Woo, Yuri Kim
BACKGROUND/OBJECTIVES: The objective of this study was to investigate the effects of vitamin C on inflammation, tumor development, and dysbiosis of intestinal microbiota in an azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced inflammation-associated early colon cancer mouse model. MATERIALS/METHODS: Male BALB/c mice were injected intraperitoneally with AOM [10 mg/kg body weight (b.w)] and given two 7-d cycles of 2% DSS drinking water with a 14 d inter-cycle interval...
April 2018: Nutrition Research and Practice
https://www.readbyqxmd.com/read/29628568/comparison-of-prophylactic-intravenous-magnesium-sulfate-with-tramadol-for-postspinal-shivering-in-elective-cesarean-section-a-placebo-controlled-randomized-double-blind-pilot-study
#8
Roopa Sachidananda, K Basavaraj, Safiya I Shaikh, G Umesh, Triveni Bhat, B Arpitha
Background and Aims: Cesarean sections are performed mostly under spinal anesthesia. Shivering is one of the distressing complications. The aim of the study was to compare the efficacy of intravenous (i.v) magnesium sulfate and tramadol with placebo (normal saline) on postspinal shivering in elective cesarean section when used as prophylaxis. Methods: One hundred and thirty-five pregnant women between 18 and 35 years age, belonging to the American Society of Anesthesiologists' physical Status II, undergoing elective cesarean section under spinal anesthesia were enrolled into the study...
January 2018: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/29628299/effectiveness-and-safety-of-sclerotherapy-for-treatment-of-low-flow-vascular-malformations-of-the-oropharyngeal-region
#9
Patrick Bourgouin, Frédéric Thomas-Chaussé, Patrick Gilbert, Marie-France Giroux, Sébastien Périgny, Louis Guertin, Josée Dubois, Gilles Soulez
PURPOSE: To evaluate the efficacy and safety of sclerotherapy with sodium tetradecyl sulfate (STS) and bleomycin for treatment of venous malformations (VMs) of the oropharyngeal region. MATERIALS AND METHODS: A retrospective study of 33 patients with 46 VMs of the buccal and pharyngolaryngeal cavity associated with impairment of eating, respiration, or elocution was performed. Individual lesions were divided based on their anterior or posterior location, using the base of the tongue as an anatomic landmark...
April 6, 2018: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/29628081/biogeochemical-cycling-of-metals-impacting-by-microbial-mobilization-and-immobilization
#10
REVIEW
Ran Jing, Birthe V Kjellerup
Microbial mobilization and immobilization processes can affect the bioavailability and mobility of metals thereby influencing their toxicity and can therefore be utilized to treat solid and liquid wastes contaminated by metals. However, the microbial mobilization and immobilization of metals depends on the microbial metabolism, the environment conditions. In this review, mobilization and immobilization of metals are discussed with regard to the presence and function of involved microorganisms and in relation to applications such as bioleaching...
April 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29627646/effective-treatment-of-oily-scum-via-catalytic-wet-persulfate-oxidation-process-activated-by-fe-2
#11
Xingzhong Yuan, Renpeng Guan, Zhibin Wu, Longbo Jiang, Yifu Li, Xiaohong Chen, Guangming Zeng
Oily scum, a hazardous by-product of petroleum industry, need to be deposed urgently to reduce environmental risks. This paper introduces catalytic wet persulfate oxidation (CWPO) process in the treatment of oily scum to realize risk relief. Under the activation of heat and Fe2+ , persulfate (PS) was decomposed into sulfate radicals and hydroxyl radicals, which played a major role on the degradation of petroleum hydrocarbons. The effects of wet air oxidation (WAO) and CWPO process on the degradation of oily scum were compared...
April 5, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29626603/peroxidase-from-jackfruit-purification-characterization-and-thermal-inactivation
#12
Yi-Ming Tao, Song Wang, Hui-Liu Luo, Wen-Wen Yan
Peroxidase (POD) from jackfruit bulb was purified using ammonium sulfate precipitation, hydrophobic interaction and gel filtration columns. The POD was a dimer with a molecular weight of 104 kDa. The Km and Vmax values for guaiacol, gallic acid and o‑phenylenediamine (OPD) were estimated. OPD was the most suitable substrate. The enzyme showed its maximum activity at pH 5.5 and 55-60 °C. The activation energy (Ea ) of heat inactivation was estimated to be 206.40 kJ/mol. The enthalpy, free energy and entropy values for the thermal inactivation were also determined...
April 4, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29625978/subgroup-characteristics-of-marine-methane-oxidizing-anme-2-archaea-and-their-syntrophic-partners-revealed-by-integrated-multimodal-analytical-microscopy
#13
Shawn E McGlynn, Grayson L Chadwick, Ariel O'Neill, Mason Mackey, Andrea Thor, Thomas J Deerinck, Mark H Ellisman, Victoria J Orphan
Phylogenetically diverse environmental ANME archaea and sulfate-reducing bacteria cooperatively catalyze the anaerobic oxidation of methane oxidation (AOM) in multi-celled consortia within methane seep environments. To better understand these cells and their symbiotic associations, we applied a suite of electron microscopy approaches including correlative<u>f</u>luorescence <u>i</u> n<u>s</u>itu <u>h</u>ybridization -<u>e</u>lectron<u>m</u>icroscopy (FISH-EM),<u>t</u>ransmission<u>e</u>lectron<u>m</u>icroscopy (TEM), and<u>s</u>erial<u>b</u>lock face scanning<u>e</u>lectron<u>m</u>icroscopy 3D reconstructions (SBEM)...
April 6, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29624877/electron-acceptors-for-anaerobic-oxidation-of-methane-drive-microbial-community-structure-and-diversity-in-mud-volcanoes
#14
Ge Ren, Anzhou Ma, Yanfen Zhang, Ye Deng, Guodong Zheng, Xuliang Zhuang, Guoqiang Zhuang, Danielle Fortin
Mud volcanoes (MVs) emit globally significant quantities of methane into the atmosphere, however, methane cycling in such environments is not yet fully understood, as the roles of microbes and their associated biogeochemical processes have been largely overlooked. Here, we used data from high-throughput sequencing of microbial 16S rRNA gene amplicons from six MVs in the Junggar Basin in northwest China to quantify patterns of diversity and characterize the community structure of archaea and bacteria. We found anaerobic methanotrophs and diverse sulfate- and iron-reducing microbes in all of the samples, and the diversity of both archaeal and bacterial communities was strongly linked to the concentrations of sulfate, iron, and nitrate, which could act as electron acceptors in anaerobic oxidation of methane (AOM)...
April 6, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29621720/elevated-nitrogen-metabolism-and-nitric-oxide-production-are-involved-in-arabidopsis-resistance-to-acid-rain
#15
Fang Qiao, Xi-Min Zhang, Xiang Liu, Juan Chen, Wen-Jun Hu, Ting-Wu Liu, Ji-Yun Liu, Chun-Quan Zhu, Kabir Ghoto, Xue-Yi Zhu, Hai-Lei Zheng
Acid rain (AR) can induce great damages to plants and could be classified into different types according to the different SO4 2- /NO3 - ratio. However, the mechanism of plants' responding to different types of AR has not been elucidated clearly. Here, we found that nitric-rich simulated AR (N-SiAR) induced less leaves injury as lower necrosis percentage, better physiological parameters and reduced oxidative damage in the leaves of N-SiAR treated Arabidopsis thaliana compared with sulfate and nitrate mixed (SN-SiAR) or sulfuric-rich (S-SiAR) simulated AR treated ones...
March 26, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29621420/regulation-of-eosinophil-recruitment-and-allergic-airway-inflammation-by-heparan-sulfate-proteoglycan-hspg-modifying-enzymes
#16
Xiao Na Ge, Idil Bastan, Sung Gil Ha, Yana G Greenberg, Jeffrey D Esko, Savita P Rao, P Sriramarao
BACKGROUND: HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 (Ndst1), an enzyme responsible for N-sulfation during HS biosynthesis, reduces allergic airway inflammation (AAI). Here, we investigated the importance of O-sulfation mediated by uronyl 2-O-sulfotransferase (Hs2st) in development of AAI relative to N-sulfation...
April 5, 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29618263/a-thermodynamic-and-kinetic-study-of-trace-iron-removal-from-aqueous-cobalt-sulfate-solutions-using-monophos-resin
#17
Guangxin Wang, Yunchao Zhao, Bin Yang, Yongfa Song
BACKGROUND: High purity cobalt has many important applications, such as magnetic recording media, magnetic recording heads, optoelectronic devices, magnetic sensors, and integrated circuits, etc. To produce 5N or higher purity cobalt in an electro-refining process, one of the challenges is to effectively reduce the Fe content of aqueous cobalt salt solution before electrolysis. This paper describes thermodynamic and kinetic investigations of the Fe adsorption process of a new sulfonated monophosphonic resin with the trade mark Monophos...
January 2018: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29617126/long-term-feeding-of-elemental-sulfur-alters-microbial-community-structure-and-eliminates-mercury-methylation-potential-in-srb-abundant-activated-sludge
#18
Jin-Ting Wang, Liang Zhang, Yuan Kang, Guanghao Chen, Feng Jiang
This study reported a novel observation that the long-term cultivation of sulfur-reducing bacteria (S0RB) from a sulfate-reducing bacteria (SRB)-abundant seeding sludge with elemental sulfur feeding, significantly shaped the microbial community structure and eliminated the mercury methylation potential in the S0RB-enriched sludge. In this study, the enrichments of SRB and S0RB from activated sludge were obtained through long-term cultivations. Subsequently, the batch tests showed that approximately 5000 μg/L Hg (II) was completely removed from the solution by both the SRB-enriched and S0RB-enriched sludge...
April 4, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29616315/two-rab-gtpases-play-different-roles-in-conidiation-trap-formation-stress-resistance-and-virulence-in-the-nematode-trapping-fungus-arthrobotrys-oligospora
#19
Xuewei Yang, Ni Ma, Le Yang, Yaqing Zheng, Zhengyi Zhen, Qing Li, Meihua Xie, Juan Li, Ke-Qin Zhang, Jinkui Yang
Rab GTPases are the largest group of the small GTPases family, which play a pivotal role in the secretion of proteins. Arthrobotrys oligospora is a representative nematode-trapping fungus that can produce adhesive networks to capture nematodes. In this study, the roles of two Rab GTPases AoRab-7A and AoRab-2 were characterized by gene knockout in the fungus A. oligospora. The disruption of AoRab-7A hindered the mycelial growth in different media, the conidiation of ΔAoRab-7A transformants was almost abolished, and the transcription of four sporulation-related genes (AbaA, FluG, Hyp1, and VosA) was downregulated compared to the wild-type strain (WT)...
April 3, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29616016/heparan-sulfate-induces-necroptosis-in-murine-cardiomyocytes-a-medical-in-silico-approach-combining-in-vitro-experiments-and-machine-learning
#20
Elisabeth Zechendorf, Phillip Vaßen, Jieyi Zhang, Ahmed Hallawa, Antons Martincuks, Oliver Krenkel, Gerhard Müller-Newen, Tobias Schuerholz, Tim-Philipp Simon, Gernot Marx, Gerd Ascheid, Anke Schmeink, Guido Dartmann, Christoph Thiemermann, Lukas Martin
Life-threatening cardiomyopathy is a severe, but common, complication associated with severe trauma or sepsis. Several signaling pathways involved in apoptosis and necroptosis are linked to trauma- or sepsis-associated cardiomyopathy. However, the underling causative factors are still debatable. Heparan sulfate (HS) fragments belong to the class of danger/damage-associated molecular patterns liberated from endothelial-bound proteoglycans by heparanase during tissue injury associated with trauma or sepsis. We hypothesized that HS induces apoptosis or necroptosis in murine cardiomyocytes...
2018: Frontiers in Immunology
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