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https://www.readbyqxmd.com/read/29053991/molecular-mechanisms-of-development-of-the-human-fetal-female-reproductive-tract
#1
Gerald R Cunha, Takeshi Kurita, Mei Cao, Joel Shen, Stanley Robboy, Laurence Baskin
Human female reproductive tract development rests mostly upon hematoxilyn and eosin stained sections despite recent advances on molecular mechanisms in mouse studies. We report application of immunohistochemical methods to explore the ontogeny of epithelial and mesenchymal differentiation markers (keratins, homobox proteins, steroid receptors), transcription factors and signaling molecules (TP63 and RUNX1) during human female reproductive tract development. Keratins 6, 7, 8, 10, 14 and 19 (KRT6, KRT7, KRT8, KRT10, KRT14, KRT19) were expressed in a temporally and spatially dynamic fashion...
July 29, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29053925/mesenchymal-stem-cell-secretome-improves-tendon-cell-viability-in-vitro-and-tendon-bone-healing-in-vivo-when-a-tissue-engineering-strategy-is-used-in-a-rat-model-of-chronic-massive-rotator-cuff-tear
#2
Nuno Sevivas, Fábio Gabriel Teixeira, Raquel Portugal, Bruno Direito-Santos, João Espregueira-Mendes, Filipe J Oliveira, Rui F Silva, Nuno Sousa, Wan Ting Sow, Nguyen Luong, Kee Woei Ng, António J Salgado
BACKGROUND: Massive rotator cuff tears (MRCTs) represent a major clinical concern, especially when degeneration and chronicity are involved, which highly compromise healing capacity. PURPOSE: To study the effect of the secretome of mesenchymal stem cells (MSCs) on tendon cells (TCs) followed by the combination of these activated TCs with an electrospun keratin-based scaffold to develop a tissue engineering strategy to improve tendon-bone interface (TBi) healing in a chronic MRCT rat model...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29051081/comparative-proteomic-analyses-using-itraq-labeling-provides-insights-into-fiber-diversity-in-sheep-and-goats
#3
Yan Li, Guangxian Zhou, Rui Zhang, Jiazhong Guo, Chao Li, Graeme Martin, Yulin Chen, Xiaolong Wang
The structural component of wool and hair fibers, such as keratin-associated proteins (KAPs), has been well described, but the genetic determinants of fiber diameter are largely unknown. Here, we have used an iTRAQ-based proteomic approach to investigate differences in protein expression among 18 samples from sheep and goats across a diverse range of fibers. We identified proteins that are differentially expressed and are associated with variation in fiber features. Proteins with different abundance are mainly keratin or keratin-associated proteins (KRTAP11-1, KRT6A, KRT38), or are related to hair growth (DSC2, DSG3, EEF2, CALML5, TCHH, SELENBP1) and fatty acid synthesis (FABP4, FABP5)...
October 16, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29050444/treatment-of-ichthyosis-vulgaris-with-a-urea-based-emulsion-videodermatoscopy-and-confocal-microscopy-evaluation
#4
Clara Benintende, Simona Boscaglia, Franco Dinotta, Francesco Lacarrubba, Giuseppe Micali
BACKGROUND: Ichthyosis vulgaris is a common disorder of keratinization caused by mutations in the filaggrin gene and clinically characterized by variable degree of xerosis. METHODS: Five patients affected by ichthyosis vulgaris and moderate to severe xerosis of the lower limbs, were treated twice daily for 30 days with an emulsion containing 10% urea, ceramides, and natural moisturizing factors (NMF). Evaluation was performed at baseline and at the end of treatment by clinical examination, Visual Analogue Scale to quantify itch, videodermatoscopy (VD), and reflectance confocal microscopy (RCM)...
December 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/29047160/mutations-in-the-genes-for-keratin-4-and-13-in-swedish-patients-with-white-sponge-nevus
#5
Maria Westin, Elham Rekabdar, Lena Blomstrand, Per Klintberg, Mats Jontell, Jairo Robledo-Sierra
BACKGROUND: White sponge nevus is a rare autosomal dominant disorder that affects the non-keratinised stratified squamous epithelium. Mutations in the genes that encode mucosa-specific keratins 4 and 13 are strongly linked to the manifestation of white sponge nevus. This study involved mutational analysis of the genes encoding keratin-4 and keratin-13 in two Swedish families with white sponge nevus. METHODS: The diagnosis of white sponge nevus was based on disease history, clinical characteristics of the lesions and, in the majority of the cases, histopathological examination...
October 19, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29046725/clinical-photographic-observation-of-plantar-corns-and-callus-associated-with-a-nominal-scale-classification-and-inter-observer-reliability-study-in-a-student-population
#6
David R Tollafield
BACKGROUND: The management of plantar corns and callus has a low cost-benefit with reduced prioritisation in healthcare. The distinction between types of keratin lesions that forms corns and callus has attracted limited interest. Observation is imperative to improving diagnostic predictions and a number of studies point to some confusion as to how best to achieve this. The use of photographic observation has been proposed to improve our understanding of intractable keratin lesions. METHODS: Students from a podiatry school reviewed photographs where plantar keratin lesions were divided into four nominal groups; light callus (Grade 1), heavy defined callus (Grade 2), concentric keratin plugs (Grade 3) and callus with deeper density changes under the forefoot (Grade 4)...
2017: Journal of Foot and Ankle Research
https://www.readbyqxmd.com/read/29044619/variation-in-crocodilian-dorsal-scute-organization-and-geometry-with-a-discussion-of-possible-functional-implications
#7
Lauren T English
Dermal ossifications, including osteoderms, are present in many vertebrates and are frequently interpreted as a defense against predators. Nevertheless, osteoderms remain ubiquitous in adult crocodilians while being absent in hatchlings, even though adults rarely experience predation. In other biological systems, increased variation, particularly fluctuating asymmetry, have proven useful for identifying biological structures likely to have evolved under relaxed selection, which in turn may inform their function...
October 17, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/29043445/the-role-of-intermediate-filaments-in-maintaining-integrity-and-function-of-intestinal-epithelial-cells-after-massive-bowel-resection-in-a-rat
#8
I Sukhotnik, Y Ben Shahar, Y Pollak, T Dorfman, H Kreizman Shefer, Z E Assi, N Mor-Vaknin, A G Coran
PURPOSE: Intermediate filaments (IFs) are a part of the cytoskeleton that extend throughout the cytoplasm of all cells and function in the maintenance of cell-shape by bearing tension and serving as structural components of the nuclear lamina. In normal intestine, IFs provide a tissue-specific three-dimensional scaffolding with unique context-dependent organizational features. The purpose of this study was to evaluate the role of IFs during intestinal adaptation in a rat model of short bowel syndrome (SBS)...
October 17, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/29043090/the-use-of-infrared-thermography-to-detect-the-stages-of-estrus-cycle-and-ovulation-time-in-anatolian-shepherd-dogs
#9
Kemal Tuna Olğaç, Ergun Akçay, Beste Çil, Burak Mehmet Uçar, Ali Daşkın
BACKGROUND: The aim of the study is to evaluate the effectiveness of thermographic monitoring, using the temperature changes of perianal and perivulvar areas for the determination of estrus in Anatolian Shepherd bitches. Fifteen bitches were used in the study. Blood and vaginal smear samples were collected and thermographic monitoring of perianal and perivulvar areas were carried out starting from proestrus to early diestrus. Also, external signs of estrus were investigated. Smear samples were evaluated by light microscopy after Diff-Quik staining method and superficial and keratinized superficial cells were determined as percentage (S + KS%)...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/29042920/screening-of-differentially-expressed-proteins-in-psoriasis-vulgaris-by-two-dimensional-gel-electrophoresis-and-mass-spectrometry
#10
Yinan Dai, Qingrui Zhang, Yi Jiang, Lu Yin, Xiaodong Zhang, Yang Chen, Xinze Cai
The aim of the present study was to elucidate differentially expressed proteins in lesional tissues of psoriasis vulgaris (PV) and normal tissues. Lesional skin tissues were collected from PV patients, along with normal skin tissues from healthy individuals. The protein content of the samples was extracted and then separated by two-dimensional gel electrophoresis (2-DGE). Any proteins that were differentially expressed in the lesional skin of PV patients compared with the healthy controls were analyzed by mass spectrometry and bioinformatics...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042651/biochemistry-and-adaptive-colouration-of-an-exceptionally-preserved-juvenile-fossil-sea-turtle
#11
Johan Lindgren, Takeo Kuriyama, Henrik Madsen, Peter Sjövall, Wenxia Zheng, Per Uvdal, Anders Engdahl, Alison E Moyer, Johan A Gren, Naoki Kamezaki, Shintaro Ueno, Mary H Schweitzer
The holotype (MHM-K2) of the Eocene cheloniine Tasbacka danica is arguably one of the best preserved juvenile fossil sea turtles on record. Notwithstanding compactional flattening, the specimen is virtually intact, comprising a fully articulated skeleton exposed in dorsal view. MHM-K2 also preserves, with great fidelity, soft tissue traces visible as a sharply delineated carbon film around the bones and marginal scutes along the edge of the carapace. Here we show that the extraordinary preservation of the type of T...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042638/carbon-nanowalls-as-a-platform-for-biological-sers-studies
#12
Pavel Dyakonov, Kirill Mironovich, Sergey Svyakhovskiy, Olga Voloshina, Sarkis Dagesyan, Andrey Panchishin, Nikolay Suetin, Victor Bagratashvili, Petr Timashev, Evgeny Shirshin, Stanislav Evlashin
Herein we report about developing new type of Surface Enhanced Raman Scattering (SERS) substrates based on Au-decorated carbon nanowalls. The designed substrates possess high specific surface area and high sensitivity. Chemical stability of Au perfectly blends with electrical properties and high value of specific surface area of carbon nanowalls. Created structures were applied to detect signals of a typical molecule used for SERS substrates testing, rhodamine 6G, which exhibits electronic absorption in the visible area of spectrum, and biomacromolecules such as tryptophan, guanine, bovine serum albumin and keratin hydrolysates, whose electronic absorption is in the ultraviolet region of spectrum and lies far from the Au plasmonic resonance...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29039047/the-expression-pattern-of-keratin-24-in-tissue-engineered-dermo-epidermal-human-skin-substitutes-in-an-in-vivo-model
#13
Agnes S Klar, Katarzyna Michalak, Sophie Böttcher-Haberzeth, Ernst Reichmann, Martin Meuli, Thomas Biedermann
AIMS AND OBJECTIVES: The use of autologous tissue-engineered skin substitutes is a promising approach to cover large skin defects in patients. Preclinical investigation is pivotal to test and improve the quality of these bio-engineered substitutes. In the skin, the epidermis, formed mainly by keratinocytes, provides the first physical barrier protecting from the environment. Proper keratinocyte differentiation and, thus, formation of a stratified epidermis is essential for this function...
October 16, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/29035818/skin-penetrating-polymeric-nanoparticles-incorporated-in-silk-fibroin-hydrogel-for-topical-delivery-of-curcumin-to-improve-its-therapeutic-effect-on-psoriasis-mouse-model
#14
Kai-Li Mao, Zi-Liang Fan, Jian-Dong Yuan, Pian-Pian Chen, Jing-Jing Yang, Jie Xu, De-Li ZhuGe, Bing-Hui Jin, Qun-Yan Zhu, Bi-Xin Shen, Yasin Sohawon, Ying-Zheng Zhao, He-Lin Xu
A poor percutaneous penetration capability for most topical anti-inflammatory drugs is one of the main causes compromising their therapeutic effects on psoriatic skin. Even though curcumin has shown a remarkable efficacy in the treatment of psoriasis, its effective penetration through the stratum corneum is still a major challenge during transdermal delivery. The aim of our study was to design skin-permeating nanoparticles (NPs) to facilitate delivery of curcumin to the deeper layers of the skin. A novel amphiphilic polymer, RRR-α-tocopheryl succinate-grafted-ε-polylysine conjugate (VES-g-ε-PLL) was synthesized and self-assembled into polymeric nanoparticles...
October 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29034342/p16-ink4a-expression-in-invasive-laryngeal-cancer
#15
Brenda Y Hernandez, Mobeen Rahman, Charles F Lynch, Wendy Cozen, Elizabeth R Unger, Martin Steinau, Trevor Thompson, Maria Sibug Saber, Sean F Altekruse, Marc T Goodman, Amy Powers, Christopher Lyu, Mona Saraiya
We examined p16 expression in tumors from a population-based sample of laryngeal cancer cases diagnosed in the U.S. Samples had been previously genotyped for HPV DNA. Overall, p16 expression was observed in laryngeal tissue from 8 of 101 (7.9%) cases. p16 expression was observed in 2 of 16 (12.5%) cases previously determined to be HPV DNA positive. The two cases dually positive for p16 and HPV DNA were non-keratinizing SCC and papillary SCC tumors that were positive for genotypes 18 and 35/89, respectively...
December 2016: Papillomavirus Research
https://www.readbyqxmd.com/read/29032358/a-rim-and-spoke-hypothesis-to-explain-the-biomechanical-roles-for-cytoplasmic-intermediate-filament-networks
#16
REVIEW
Roy A Quinlan, Nicole Schwarz, Reinhard Windoffer, Christine Richardson, Tim Hawkins, Joshua A Broussard, Kathleen J Green, Rudolf E Leube
Textbook images of keratin intermediate filament (IF) networks in epithelial cells and the functional compromization of the epidermis by keratin mutations promulgate a mechanical role for this important cytoskeletal component. In stratified epithelia, keratin filaments form prominent radial spokes that are focused onto cell-cell contact sites, i.e. the desmosomes. In this Hypothesis, we draw attention to a subset of keratin filaments that are apposed to the plasma membrane. They form a rim of filaments interconnecting the desmosomes in a circumferential network...
October 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29028846/periodontal-indices-and-status-in-34-growing-patients-with-unilateral-cleft-lip-and-palate-a-split-mouth-study
#17
Paweł Plakwicz, Beata Wyrębek, Renata Górska, Dorota Cudziło
Periodontal examination in growing patients with unilateral cleft lip and palate was performed in a split-mouth study design. Higher plaque acumulation and bleeding indices were recorded for teeth on the cleft side. Pocket probing depth (PPD) and clinical attachment level (CAL) values were higher at lateral incisors and canines adjacent to the cleft. Keratinized gingiva and depth of the vestibule were lower adjacent to the cleft. This study shows that growing patients with clefts present differences regarding periodontal parameters between the cleft and the control side...
November 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/29028840/polymorphism-of-keratin-1-associates-with-systemic-lupus-erythematosus-and-systemic-sclerosis-in-a-south-chinese-population
#18
Weiguang Luo, Bin Zhou, Qizhi Luo, Huilong Fang, Xiaoxia Zuo, Yizhou Zou
Both systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) diseases are related to the genetic and environmental factors, causing damage to the skin. The mutations of keratin 1 gene (KRT1) were reported to associate with skin diseases. The single-nucleotide polymorphism (SNP, rs14024) and the indel polymorphism (cds-indel, rs267607656), consisting mostly of the common haplotypes and could be used for genotyping of KRT1. We used the PCR with sequence specific primers (PCR-SSP) to determine the genotype of KRT1 in 164 SLE, 99 SSc patients, and 418 healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/29027377/contribution-of-extracellular-matrix-and-signal-mechanotransduction-to-epithelial-cell-damage-in-inflammatory-bowel-disease-patients-a-proteomic-study
#19
Manuela Moriggi, Luca Pastorelli, Enrica Torretta, Gian Eugenio Tontini, Daniele Capitanio, Stefano Ferrero Bogetto, Maurizio Vecchi, Cecilia Gelfi
This study utilized 2D-DIGE, isotope-coded protein labelling (ICPL) and biochemical assays to characterize protein alteration in Ulcerative Colitis (UC) and Crohn's Disease (CD) in human epithelial cell and mucosal biopsies in Inflammatory Bowel Disease (IBD)-affected patients. The aim of this study is to identify the key molecular signatures involved in epithelial cell structure of IBDs. In non-inflamed UC (QUC) keratins, vimentin and FAK increased, whereas vinculin and de-tyrosinated α-tubulin decreased; inflammation (IUC) exacerbated molecular changes, being COL6A1, tenascin-C and vimentin increased...
October 13, 2017: Proteomics
https://www.readbyqxmd.com/read/29026647/progress-in-development-of-bioderived-materials-for-dermal-wound-healing
#20
REVIEW
Lin-Cui Da, Yi-Zhou Huang, Hui-Qi Xie
Treatment of acute and chronic wounds is one of the primary challenges faced by doctors. Bioderived materials have significant potential clinical value in tissue injury treatment and defect reconstruction. Various strategies, including drug loading, addition of metallic element(s), cross-linking and combining two or more distinct types of materials with complementary features, have been used to synthesize more suitable materials for wound healing. In this review, we describe the recent developments made in the processing of bioderived materials employed for cutaneous wound healing, including newly developed materials such as keratin and soy protein...
October 2017: Regenerative Biomaterials
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