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https://www.readbyqxmd.com/read/29040916/versatile-on-stage-microfluidic-system-for-long-term-cell-culture-micromanipulation-and-time-lapse-assays
#1
Yao-Xiong Huang, Chun-Lan He, Ping Wang, Yan-Ting Pan, Wei-Wei Tuo, Cheng-Can Yao
We report here a versatile on-stage microfluidic cell culture and assay system which is compatible with different microscopes and sensors, can simultaneously perform steps of long term cell culture, high throughput time lapse cell assays/imaging, and cell micromanipulations. With the system, we cultured a variety of cells for different periods of time and monitored their cell morphology, migration and division. We also performed a series non-invasive real time in situ time lapse assays and micromanipulations on different cells...
October 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29040801/molecular-level-insight-into-the-interaction-of-phospholipid-bilayers-with-cellulose
#2
Andrei Yu Kostritskii, Dmitry Alekseevich Tolmachev, Natalia Viacheslavovna Lukasheva, Andrey A Gurtovenko
A molecular-level insight into the interactions of phospholipid molecules with cellulose is crucial for the development of novel cellulose-based materials for wound dressing. Here we employ the state-of-the-art computer simulations to unlock for a first time the molecular mechanisms behind such interactions. To this end, we performed a series of atomic-scale molecular dynamics simulations of phospholipid bilayers on a crystalline cellulose support at various hydration levels of the bilayer leaflets next to the cellulose surface...
October 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29040675/fluctuation-localization-imaging-based-fluorescence-in-situ-hybridization-flifish-for-accurate-detection-and-counting-of-rna-copies-in-single-cells
#3
Yi Cui, Dehong Hu, Lye Meng Markillie, William B Chrisler, Matthew J Gaffrey, Charles Ansong, Lori Sussel, Galya Orr
Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide probes, and background noise. Thus, the precision in smFISH is often compromised by partial or nonspecific probe binding and tissue autofluorescence, especially when only a small number of probes can be fitted to the target transcript...
October 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040644/displaying-and-analyzing-quality-improvement-data
#4
Patrick W Brady, Michael J Tchou, Lilliam Ambroggio, Amanda C Schondelmeyer, Erin E Shaughnessy
In this second article in the quality improvement (QI) methods series, we discuss how data are best displayed and analyzed in QI projects while focusing on some similarities with and differences from traditional clinical research. We demonstrate why displaying data over time on a run or control chart is superior to using pre-post analysis for QI studies. We introduce several types of statistical process control charts for data commonly collected during QI programs and provide guidance on how to use the proper chart...
October 5, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/29040409/towards-dynamic-genome-scale-models
#5
David Gilbert, Monika Heiner, Yasoda Jayaweera, Christian Rohr
The analysis of the dynamic behaviour of genome-scale models of metabolism (GEMs) currently presents considerable challenges because of the difficulties of simulating such large and complex networks. Bacterial GEMs can comprise about 5000 reactions and metabolites, and encode a huge variety of growth conditions; such models cannot be used without sophisticated tool support. This article is intended to aid modellers, both specialist and non-specialist in computerized methods, to identify and apply a suitable combination of tools for the dynamic behaviour analysis of large-scale metabolic designs...
October 13, 2017: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/29040398/correlation-of-blood-pressure-variability-as-measured-by-clinic-self-measurement-at-home-and-ambulatory-blood-pressure-monitoring
#6
J Abellán-Huerta, L Prieto-Valiente, S Montoro-García, J Abellán-Alemán, F Soria-Arcos
Background: Blood pressure variability (BPV) has been postulated as a potential predictor of cardiovascular outcomes. No agreement exists as to which measurement method is best for BPV estimation. We attempt to assess the correlation between BPV obtained at the doctor's office, self-measurement at home (SMBP) and ambulatory BP monitoring (ABPM). Methods: Eight weekly clinic BP measurements, two SMBP series and one 24-hour ABPM recording were carried out in a sample of treated hypertensive patients...
October 10, 2017: American Journal of Hypertension
https://www.readbyqxmd.com/read/29040386/robust-distributed-lag-models-using-data-adaptive-shrinkage
#7
Yin-Hsiu Chen, Bhramar Mukherjee, Sara D Adar, Veronica J Berrocal, Brent A Coull
Distributed lag models (DLMs) have been widely used in environmental epidemiology to quantify the lagged effects of air pollution on an outcome of interest such as mortality or cardiovascular events. Generally speaking, DLMs can be applied to time-series data where the current measure of an independent variable and its lagged measures collectively affect the current measure of a dependent variable. The corresponding distributed lag (DL) function represents the relationship between the lags and the coefficients of the lagged exposure variables...
October 12, 2017: Biostatistics
https://www.readbyqxmd.com/read/29040076/total-coliform-and-escherichia-coli-contamination-in-rural-well-water-analysis-for-passive-surveillance
#8
Jesse Invik, Herman W Barkema, Alessandro Massolo, Norman F Neumann, Sylvia Checkley
With increasing stress on our water resources and recent waterborne disease outbreaks, understanding the epidemiology of waterborne pathogens is crucial to build surveillance systems. The purpose of this study was to explore techniques for describing microbial water quality in rural drinking water wells, based on spatiotemporal analysis, time series analysis and relative risk mapping. Tests results for Escherichia coli and coliforms from private and small public well water samples, collected between 2004 and 2012 in Alberta, Canada, were used for the analysis...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29039923/a-charge-density-tunable-three-two-dimension-polymer-graphene-oxide-heterogeneous-nanoporous-membrane-for-ion-transport
#9
Xuanbo Zhu, Yahong Zhou, Junran Hao, Bin Bao, Xiujie Bian, Xiangyu Jiang, Jinhui Pang, Haibo Zhang, Zhenhua Jiang, Lei Jiang
Design and fabrication of robust nanoporous membrane in large-scale is still being a challenge and is of fundamental importance for practical applications. Here, a robust three/two dimension - polymer/graphene oxide heterogeneous nanoporous membrane in large-scale via the self-assembly approach is constructed, by chemically designing a robust charge-density-tunable nanoporous ionomer with uniform pore size. To obtain nanoporous polymer which maintains high mechanical strength and promotes multi-functionality, we design a series of amphiphilic copolymer by introducing a positively charged pyridine moiety into engineering polymer polyphenylsulphone (PPSU)...
October 17, 2017: ACS Nano
https://www.readbyqxmd.com/read/29036840/efficacy-of-memory-aids-after-traumatic-brain-injury-a-single-case-series
#10
Hannah R Bos, Duncan R Babbage, Janet M Leathem
BACKGROUND: Individuals living with traumatic brain injury commonly have difficulties with prospective memory- the ability to remember a planned action at the intended time. Traditionally a memory notebook has been recommended as a compensatory memory aid. Electronic devices have the advantage of providing a cue at the appropriate time to remind participants to refer to the memory aid and complete tasks. Research suggests these have potential benefit in neurorehabilitation. OBJECTIVE: This study aimed to investigate the efficacy of a memory notebook and specifically a smartphone as a compensatory memory aid...
October 10, 2017: NeuroRehabilitation
https://www.readbyqxmd.com/read/29036756/acupuncture-or-acupressure-for-induction-of-labour
#11
REVIEW
Caroline A Smith, Mike Armour, Hannah G Dahlen
BACKGROUND: This is one of a series of reviews of methods of cervical ripening and labour induction. The use of complementary therapies is increasing. Women may look to complementary therapies during pregnancy and childbirth to be used alongside conventional medical practice. Acupuncture involves the insertion of very fine needles into specific points of the body. Acupressure is using the thumbs or fingers to apply pressure to specific points. The limited observational studies to date suggest acupuncture for induction of labour has no known adverse effects to the fetus, and may be effective...
October 17, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29036751/-diagnosis-and-management-of-vestibular-schwannomas-an-interdisciplinary-challenge
#12
Steffen Rosahl, Christopher Bohr, Michael Lell, Klaus Hamm, Heinrich Iro
Vestibular schwannomas expand slowly in the internal auditory canal, in the cerebellopontine angle, inside the cochlear and the labyrinth. Larger tumors can displace and compress the brainstem. With an annual incidence of 1:100 000 vestibular schwannoma represent 6-7% of all intracranial tumors. In the cerebellopontine angle they are by far the most neoplasm with 90% of all lesions located in this region. Magnetic resonance imaging (MRI), audiometry, and vestibular diagnostics are the mainstays of the clinical workup for patients harboring tumors...
April 2017: Laryngo- Rhino- Otologie
https://www.readbyqxmd.com/read/29036526/dynapho-a-web-platform-for-inferring-the-dynamics-of-time-series-phosphoproteomics
#13
Chia-Lang Hsu, Jian-Kai Wang, Pei-Chun Lu, Hsuan-Cheng Huang, Hsueh-Fen Juan
Summary: Large-scale phosphoproteomics studies have improved our understanding of dynamic cellular signaling, but the downstream analysis of phosphoproteomics data is still a bottleneck. We develop DynaPho, a useful web-based tool providing comprehensive and in-depth analyses of time-course phosphoproteomics data, making analysis intuitive and accessible to non-bioinformatics experts. The tool currently implements five analytic modules, which reveal the transition of biological pathways, kinase activity, dynamics of interaction networks and the predicted kinase-substrate associations...
July 7, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29036501/multi-year-monitoring-of-radon-in-boreholes-at-the-modra-geophysical-observatory-slovakia
#14
I Smetanová, G Steinitz, K Holý
Long-term radon monitoring was performed in two boreholes, at a depth of 13 m in the 40 m deep V-2 borehole (August 2003-September 2005), and at 3 m depth in the 10 m deep V-3 borehole (August 2003-April 2008). Diurnal, multi-day and annual variations in radon time-series were observed. Daily average of radon activity in V-2 borehole was significantly higher and ranged from 6.5 to 383.7 kBq/m3, while in V-3 borehole only between 1.2 and 139.4 kBq/m3. The seasonal pattern was more pronounced in V-3 time series, with the maximum occurring from October to March...
September 19, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29036347/signal-decomposition-and-analysis-for-the-identification-of-periodic-and-anomalous-phenomena-in-radon-time-series
#15
C Sabbarese, F Ambrosino, F De Cicco, M Pugliese, M Quarto, V Roca
This work concerns continuous monitoring of radon and thoron specific activity in soil gas within the framework of identifying possible anomalies. It is based on the analysis of a medium-term data record obtained from soil gas in an area of geophysical interest. The RaMonA spectrometric system is also used to measure the climatic parameters and a specific analysis of the alpha spectra is performed to better determine the alpha lines intensity. Since radon emission is also influenced by meteorological parameters, it is mandatory to differentiate the changes due to the deep phenomena...
September 23, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29036327/measurement-of-incorporation-kinetics-of-non-fluorescent-native-nucleotides-by-dna-polymerases-using-fluorescence-microscopy
#16
Matthew T Walsh, Xiaohua Huang
We describe a method for measuring the single-turnover incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases. Time-lapse total internal reflection fluorescence (TIRF) microscopy is used to directly measure the kinetics of single-base nucleotide incorporation into primed DNA templates covalently attached to the surface of a glass coverslip using a fixed ratio of a native nucleotide and a corresponding fluorescently labeled nucleotide over a series of total nucleotide concentrations...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036114/stochastic-calculus-analysis-of-optical-time-of-flight-range-imaging-and-estimation-of-radial-motion
#17
Lee Streeter
Time-of-flight range imaging is analyzed using stochastic calculus. Through a series of interpretations and simplifications, the stochastic model leads to two methods for estimating linear radial velocity: maximum likelihood estimation on the transition probability distribution between measurements, and a new method based on analyzing the measured correlation waveform and its first derivative. The methods are tested in a simulated motion experiment from (-40)-(+40)  m/s, with data from a camera imaging an object on a translation stage...
July 1, 2017: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/29035986/long-term-outcome-of-mini-open-surgical-decompression-for-chronic-exertional-compartment-syndrome-of-the-forearm-in-professional-motorcycling-riders
#18
Giorgio Gondolini, Paolo Schiavi, Francesco Pogliacomi, Francesco Ceccarelli, Tommaso Antonetti, Michele Zasa
OBJECTIVE: To evaluate results of mini-open fasciotomy (MOF) in high-level motorcycling or motocross riders with chronic exertional compartment syndrome (CECS) at long-term follow-up (minimum 5 years). DESIGN: Case series. LEVEL OF EVIDENCE: IV. SETTING: University Hospital/Private Practice. PATIENTS: Fifty-four professional motorcycling riders treated with MOF for a CECS of the forearm from January 2006 to June 2011...
October 10, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/29035899/driveline-site-is-not-a-predictor-of-infection-following-ventricular-assist-device-implantation
#19
Billie-Jean Martin, Jessica G Y Luc, Michiko Maruyama, Roderick MacArthur, Angela R Bates, Holger Buchholz, Darren H Freed, Jennifer Conway
Driveline infections (DLI) remain a major source of morbidity for patients requiring long-term ventricular assist device (VAD) support. We aimed to assess whether VAD driveline exit site (DLES) (abdomen versus chest wall) is associated with DLI. All adult patients who underwent insertion of a Heartware HVAD® or Heartmate II (HMII) between 2009 and 2016 were included. DLI was defined as clinical evidence of DLI accompanied by a positive bacterial swab and need for antibiotics. Competing risks analysis was used to assess the association between patient characteristics and DLI...
October 13, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29035783/radiotracer-investigation-on-the-measurement-of-residence-time-distribution-in-an-ethyl-acetate-reactor-system-with-a-large-recycle-ratio
#20
Arghya Datta, Raj Kumar Gupta, Sunil Goswami, Vijay Kumar Sharma, Haripada Bhunia, Damandeep Singh, Harish Jagat Pant
A radiotracer investigation was carried out on the measurement of residence time distribution (RTD) of process fluid in an industrial-scale ethyl acetate reactor system, which consists of two independent reactors with recirculation and connected in series with each other. Bromine-82 as ammonium bromide was used as the radiotracer for the RTD experiments at different operating conditions. The individual reactors and the overall reactor system were modelled using physically representative phenomenological models comprising of continuously stirred tank reactors (CSTRs)...
September 25, 2017: Applied Radiation and Isotopes
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